CN105432181A - Method for promoting germination of seed of gomuti palm - Google Patents
Method for promoting germination of seed of gomuti palm Download PDFInfo
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- CN105432181A CN105432181A CN201510923276.1A CN201510923276A CN105432181A CN 105432181 A CN105432181 A CN 105432181A CN 201510923276 A CN201510923276 A CN 201510923276A CN 105432181 A CN105432181 A CN 105432181A
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- gomuti palm
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a method for promoting germination of the seed of gomuti palm. The method comprises the following steps: step 1, uniformly mixing the seeds of gomuti palm with wet perlite according to a volume ratio of 1: 3, sealing the obtained mixture and subjecting the mixture to lamination at 10 to 15 DEG C for 90 d so as to obtain laminated gomuti palm seeds; and step 2, filling a brown paper bag with the laminated gomuti palm seeds, placing the brown paper bag in a drier equipped with drying silica gel and carrying out drying at room temperature for 3 h so as to obtain the dried gomuti palm seeds. The method has the advantages of simple operation, low cost, easiness in popularization, capacity of improving the germination rate of the seeds, etc.
Description
Technical field
The present invention relates to a kind of method promoting seed germination.More particularly, the present invention relates to a kind of method promoting gomuti palm seed germination.
Background technology
Gomuti palm is Palmae Arenga arbor, inflorescence juice can granulated sugar processed, the starch that medulla extracts is called gomuti palm powder, have without features such as fat, low calorie, high microsteping, and the trace element containing multiple needed by human such as copper, iron, zinc, have the function of remuval of damp and hot and nourishing, infantile malnutrition, heating, dysentery, laryngopharyngitis etc. being had to effect of supplemental treatment, is the treasure in Forest Nutrition food.In addition, gomuti palm is a kind of multiduty nonwood forest trees in Southwestern China area, is again a kind of excellent afforestation seeds of stony desertification tor.
Gomuti palm is wild in Mountainous Area, poor growth, plant after being cut again without sprouting ability again.In recent years, gomuti palm tree in blocks is rarely found, and some places are almost endangered.Have no its vegetative propagation successfully to report, production is all bred with seed.Gomuti palm seed ovum shape prismatic, thousand kernel weight 2955.8g.We find that the seed dormant reason of gomuti palm mainly exists germination inhibitor in test, belong to physiological dormancy.The undressed seed germination cycle is long, and germination rate is very low, and the germination rate of 150 days is 35%.Seed difficulty is sprouted has become its serious problems effectively utilized further of restriction.
At present, mainly lamination, seed soaking or the gibberellin process of the seed dormant method of physiological is removed in production, land Zu Zheng etc. shows the research of gomuti palm seed, and after 80 DEG C of warm water and gibberellin process, the germination rate of 150 days is 48% and 34%, fails to significantly improve sprout time and the germination rate of seed.Therefore, a kind of method more effectively improving gomuti palm seed germination is needed badly.
Summary of the invention
The object of this invention is to provide a kind of method promoting gomuti palm seed germination, the method have simple to operate, cost is low, be easy to promote and can the advantages such as percentage of seedgermination be improved.
In order to realize, according to these objects of the present invention and other advantage, providing a kind of method promoting gomuti palm seed germination, comprise the following steps:
Step one, by gomuti palm seed with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10-15 DEG C, obtains the gomuti palm seed after lamination;
Step 2, the gomuti palm seed after described lamination to be loaded after in kraft paper bag, be placed in the drier that dry silica gel is housed, drying at room temperature 3h, obtain dry gomuti palm seed.
Preferably, in the method for described promotion gomuti palm seed germination, described wet perlite is obtained after being mixed by the perlite of 1 weight portion and the sterile water of 1.4-2 weight portion.
Preferably, in the method for described promotion gomuti palm seed germination, the gomuti palm seed after lamination described in described drier and the volume ratio of described dry silica gel are 1:100.
Preferably, in the method for described promotion gomuti palm seed germination, before being mixed with described wet perlite by described gomuti palm seed in described step one, also comprise the step of described gomuti palm seed being carried out to sterilization, its concrete steps are:
Described gomuti palm seed is put into mass fraction be 0.1% liquor potassic permanganate soak 5min after, take out, with aseptic water washing 2-3 time.
Preferably, in the method for described promotion gomuti palm seed germination, also comprise:
After sterilization being carried out to described gomuti palm seed in described step one, before described gomuti palm seed is mixed with described wet perlite, the gas-phase silica that volume is described wet perlite volume 20-30% is added in described wet perlite, wherein, the described gas-phase silica of half is absorbed with deadweight 10-20 50% carbendazol wettable powder 200-300 times liquid doubly, and second half described gas-phase silica is absorbed with deadweight 3-5 2.5% pleocidin suspension 1000-1500 times liquid doubly.
Preferably, in the method for described promotion gomuti palm seed germination, also comprise:
Step 3, carry out sterilization to the gomuti palm seed of drying, its concrete steps are:
First the gomuti palm seed of drying is put into volume fraction be 75% ethanol water soak 0.5min, take out, then put into mass fraction be 1% aqueous sodium hypochlorite solution soak 5min, take out, with aseptic water washing 3 times, obtain the gomuti palm seed of the drying after sterilization.
Preferably, in the method for described promotion gomuti palm seed germination, also comprise:
Step 4, the gomuti palm seed of the drying after the sterilization obtained in step 3 carried out to sprouting and cultivate, its concrete steps are:
Be that in the culture dish of 13cm, laying depth is after the perlite of 1-2cm equably at diameter, 100-110mL sterile water is added in culture dish, again the gomuti palm seed of the drying after 50 described sterilizations is placed in culture dish, mix with perlite, sealed by culture dish with sealed membrane afterwards, at 30 DEG C, every day first cultivates 12h under illumination condition, 12h is cultivated again, continuous culture 90 days under dark condition.
Preferably, in the method for described promotion gomuti palm seed germination, in step 4, also comprise:
Lay perlite in described culture dish before, bottom even laying depth to described culture dish is the medical stone ball of 0.8-1cm, and in culture dish, pour the 50% carbendazol wettable powder 100-150 times liquid of 10-15g into, on described medical stone ball, lay one deck filter paper identical with culture dish diameter afterwards, perlite is laid on described filter paper;
After the gomuti palm seed of the drying after sterilization is placed in culture dish, before mixing with perlite, the water-loss reducer being absorbed with deadweight 200-300 times of bactericide of 10-20g is added in perlite, mix with the gomuti palm seed of the drying after perlite and sterilization afterwards, the most backward perlitic surface uniform sprays the glycerine of 5-10g, wherein, described bactericide is made up of the component of following parts by weight: lysozyme 0.2-0.3 part, lywallzyme 0.3-0.5 part, shitosan 0.1-0.2 part, pyroligneous liquor 1-3 part and water 20-30 part.
Preferably, in the method for described promotion gomuti palm seed germination, the bottom of described culture dish is coated with photocatalyst, and the method for described promotion gomuti palm seed germination, also comprises:
With sealed membrane by after described culture dish sealing, when every day cultivates under illumination condition, with the bottom 3-5min of culture dish described in UV-irradiation, Continuous irradiation 90 days.
The present invention at least comprises following beneficial effect:
The present invention was by the Stratificated treatment of 90 days and gomuti palm seed is placed in drier before sprouting and carries out drying process, effectively can promote sprouting and the seedling of gomuti palm seed, improve the germination rate of gomuti palm seed.
The present invention have cost low, without dangerous, be easy to manual operation, pollution-free, be easy to the advantages such as popularization, the seed seedling-raising of gomuti palm, large-scale planting are of very high actual application value.
The present invention is before lamination, add in wet perlite and have the white black wood charcoal of gas phase, its be adsorbed with can sterilization carbendazol wettable powder and can many rhzomorphs suspending agent of desinsection, gas-phase silica has huge specific surface area, surperficial many meso-hole structures and superpower adsorption capacity and unusual physicochemical property, more carbendazim, many rhzomorphs and moisture etc. can be adsorbed, and in lamination process slow releasing, make gomuti palm seed be in aseptic, in humidity adapt circumstance, improve the germination rate of gomuti palm seed without worm.
The present invention is first equipped with medical stone ball in culture dish, and in medical stone ball, poured the more much higher bacterium spirit of concentration into, medical stone ball has great adsorptivity, energy adsorption moisture and medicament, also there is certain adsorption capacity to bacterium, and water can be activated, a large amount of mineral element of stripping, be conducive to seed germination, and can prevent bottom culture dish mouldy.
The present invention adds the water-loss reducer having and absorb bactericide in culture dish, and bactericide and water can slowly release by it, makes gomuti palm seed be in aseptic growing environment, improves germination rate.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to specification word to make those skilled in the art.
It should be noted that experimental technique described in following embodiment if no special instructions, is conventional method, described reagent and material, if no special instructions, all can obtain from commercial channels.
Embodiment 1
The invention provides a kind of method promoting gomuti palm seed germination, comprise the following steps:
Step one, by gomuti palm seed with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after lamination; Stratificated treatment can remove seed dormancy, promotes seed germination.
Step 2, the gomuti palm seed after described lamination to be loaded after in kraft paper bag, be placed in the drier that dry silica gel is housed, drying at room temperature 3h, obtain dry gomuti palm seed.Before sprouting is cultivated, some dehydration processing are carried out to seed, suitably can provide germination rate.
In the method for described promotion gomuti palm seed germination, described wet perlite is obtained after being mixed by the perlite of 1 weight portion and the sterile water of 1.4 weight portions.
In the method for described promotion gomuti palm seed germination, the gomuti palm seed after lamination described in described drier and the volume ratio of described dry silica gel are 1:100.
In the method for described promotion gomuti palm seed germination, before being mixed with described wet perlite by described gomuti palm seed in described step one, also comprise the step of described gomuti palm seed being carried out to sterilization, its concrete steps are:
Described gomuti palm seed is put into mass fraction be 0.1% liquor potassic permanganate soak 5min after, take out, with aseptic water washing 2 times.Sterilization is carried out to the gomuti palm seed gathered, the germ that seed carries can be reduced or eliminated.
In the method for described promotion gomuti palm seed germination, also comprise:
After sterilization being carried out to described gomuti palm seed in described step one, before described gomuti palm seed is mixed with described wet perlite, the gas-phase silica that volume is described wet perlite volume 20% is added in described wet perlite, wherein, the described gas-phase silica of half is absorbed with 50% carbendazol wettable powder, 200 times of liquid of deadweight 10 times, and second half described gas-phase silica is absorbed with 2.5% pleocidin suspension, 1000 times of liquid of deadweight 3 times.50% carbendazol wettable powder, the 200 times of liquid absorbed and 2.5% pleocidin suspension, 1000 times of liquid can slowly release by gas-phase silica, the impact of medicament on seed germination of higher concentration can be prevented, again can longer for seed germination provides suitable growing environment.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 3, carry out sterilization to the gomuti palm seed of drying, its concrete steps are:
First the gomuti palm seed of drying is put into volume fraction be 75% ethanol water soak 0.5min, take out, then put into mass fraction be 1% aqueous sodium hypochlorite solution soak 5min, take out, with aseptic water washing 3 times, obtain the gomuti palm seed of the drying after sterilization.Sterilization is carried out to the gomuti palm seed of drying, prevents dry gomuti palm seed from carrying more bacterium, cause and sprout the mold of seed in incubation, reduce germination rate.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 4, the gomuti palm seed of the drying after the sterilization obtained in step 3 carried out to sprouting and cultivate, its concrete steps are:
Be that in the culture dish of 13cm, laying depth is after the perlite of 1cm equably at diameter, 100mL sterile water is added in culture dish, again the gomuti palm seed of the drying after 50 described sterilizations is placed in culture dish, mix with perlite, sealed by culture dish with sealed membrane afterwards, at 30 DEG C, every day first cultivates 12h under illumination condition, 12h is cultivated again, continuous culture 90 days under dark condition.
In the method for described promotion gomuti palm seed germination, in step 4, also comprise:
Lay perlite in described culture dish before, bottom even laying depth to described culture dish is the medical stone ball of 0.8cm, and in culture dish, pour 50% carbendazol wettable powder, 100 times of liquid of 10g into, on described medical stone ball, lay one deck filter paper identical with culture dish diameter afterwards, perlite is laid on described filter paper; Sprout in incubation, moisture can gradually to the bottom lamination of culture dish, cause bottom culture dish easily mouldy, medical stone ball is set bottom culture dish, medical stone ball energy attracts bacteria, and in culture dish, poured 50% carbendazol wettable powder, 100 times of liquid into, medical stone ball can absorb moisture and 50% carbendazol wettable powder, 100 times of liquid, and the medicament of absorption is slowly released, can ensure that the bottom of culture dish is in germ-free condition.
After the gomuti palm seed of the drying after sterilization is placed in culture dish, before mixing with perlite, the water-loss reducer being absorbed with deadweight 200 times of bactericide of 10g is added in perlite, mix with the gomuti palm seed of the drying after perlite and sterilization afterwards, the most backward perlitic surface uniform sprays the glycerine of 5g, wherein, described bactericide is made up of the component of following parts by weight: lysozyme 0.2 part, lywallzyme 0.3 part, shitosan 0.1 part, 1 part, pyroligneous liquor and 20 parts, water.Lysozyme can make bacteria cell wall break, and also directly can be combined with electronegative virus protein, forms double salt, make virally inactivated with DNA, RNA, apoprotein.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and sprouting the temperature of cultivating is 30 DEG C, and lywallzyme thus can be made to be in suitable hydrolysis temperature.Shitosan has bacteriostatic activity.Pyroligneous liquor can suppress the breeding of miscellaneous bacteria, has fungi-proofing, effect of insect protected, and in pyroligneous liquor, acetic acid content is higher, and solution, in acid, can dissolve shitosan, and make lysozyme and lywallzyme be in suitable sour environment, improve enzymolysis efficiency.Glycerine can ensure that the seed on culture dish top is in suitable humidity.
In the method for described promotion gomuti palm seed germination, the bottom of described culture dish is coated with photocatalyst, and the method for described promotion gomuti palm seed germination, also comprises:
With sealed membrane by after described culture dish sealing, when every day cultivates under illumination condition, with the bottom 3min of culture dish described in UV-irradiation, Continuous irradiation 90 days.Photocatalyst effectively can kill various bacteria under the irradiation of ultraviolet light, and the toxin that bacterium or fungi can be discharged decomposes and harmless treatment, also can prevent bottom culture dish mouldy further.
Embodiment 2
The invention provides a kind of method promoting gomuti palm seed germination, comprise the following steps:
Step one, by gomuti palm seed with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 15 DEG C, obtains the gomuti palm seed after lamination; Stratificated treatment can remove seed dormancy, promotes seed germination.
Step 2, the gomuti palm seed after described lamination to be loaded after in kraft paper bag, be placed in the drier that dry silica gel is housed, drying at room temperature 3h, obtain dry gomuti palm seed.Before sprouting is cultivated, some dehydration processing are carried out to seed, suitably can provide germination rate.
In the method for described promotion gomuti palm seed germination, described wet perlite is obtained after being mixed by the perlite of 1 weight portion and the sterile water of 2 weight portions.
In the method for described promotion gomuti palm seed germination, the gomuti palm seed after lamination described in described drier and the volume ratio of described dry silica gel are 1:100.
In the method for described promotion gomuti palm seed germination, before being mixed with described wet perlite by described gomuti palm seed in described step one, also comprise the step of described gomuti palm seed being carried out to sterilization, its concrete steps are:
Described gomuti palm seed is put into mass fraction be 0.1% liquor potassic permanganate soak 5min after, take out, with aseptic water washing 3 times.Sterilization is carried out to the gomuti palm seed gathered, the germ that seed carries can be reduced or eliminated.
In the method for described promotion gomuti palm seed germination, also comprise:
After sterilization being carried out to described gomuti palm seed in described step one, before described gomuti palm seed is mixed with described wet perlite, the gas-phase silica that volume is described wet perlite volume 30% is added in described wet perlite, wherein, the described gas-phase silica of half is absorbed with 50% carbendazol wettable powder, 300 times of liquid of deadweight 20 times, and second half described gas-phase silica is absorbed with 2.5% pleocidin suspension, 1500 times of liquid of deadweight 5 times.50% carbendazol wettable powder, the 300 times of liquid absorbed and 2.5% pleocidin suspension, 1500 times of liquid can slowly release by gas-phase silica, the impact of medicament on seed germination of higher concentration can be prevented, again can longer for seed germination provides suitable growing environment.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 3, carry out sterilization to the gomuti palm seed of drying, its concrete steps are:
First the gomuti palm seed of drying is put into volume fraction be 75% ethanol water soak 0.5min, take out, then put into mass fraction be 1% aqueous sodium hypochlorite solution soak 5min, take out, with aseptic water washing 3 times, obtain the gomuti palm seed of the drying after sterilization.Sterilization is carried out to the gomuti palm seed of drying, prevents dry gomuti palm seed from carrying more bacterium, cause and sprout the mold of seed in incubation, reduce germination rate.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 4, the gomuti palm seed of the drying after the sterilization obtained in step 3 carried out to sprouting and cultivate, its concrete steps are:
Be that in the culture dish of 13cm, laying depth is after the perlite of 2cm equably at diameter, 110mL sterile water is added in culture dish, again the gomuti palm seed of the drying after 50 described sterilizations is placed in culture dish, mix with perlite, sealed by culture dish with sealed membrane afterwards, at 30 DEG C, every day first cultivates 12h under illumination condition, 12h is cultivated again, continuous culture 90 days under dark condition.
In the method for described promotion gomuti palm seed germination, in step 4, also comprise:
Lay perlite in described culture dish before, bottom even laying depth to described culture dish is the medical stone ball of 1cm, and in culture dish, pour 50% carbendazol wettable powder, 150 times of liquid of 15g into, on described medical stone ball, lay one deck filter paper identical with culture dish diameter afterwards, perlite is laid on described filter paper; Sprout in incubation, moisture can gradually to the bottom lamination of culture dish, cause bottom culture dish easily mouldy, medical stone ball is set bottom culture dish, medical stone ball energy attracts bacteria, and in culture dish, poured 50% carbendazol wettable powder, 150 times of liquid into, medical stone ball can absorb moisture and 50% carbendazol wettable powder, 150 times of liquid, and the medicament of absorption is slowly released, can ensure that the bottom of culture dish is in germ-free condition.
After the gomuti palm seed of the drying after sterilization is placed in culture dish, before mixing with perlite, the water-loss reducer being absorbed with deadweight 300 times of bactericide of 20g is added in perlite, mix with the gomuti palm seed of the drying after perlite and sterilization afterwards, the most backward perlitic surface uniform sprays the glycerine of 10g, wherein, described bactericide is made up of the component of following parts by weight: lysozyme 0.3 part, lywallzyme 0.5 part, shitosan 0.2 part, 3 parts, pyroligneous liquor and 30 parts, water.Lysozyme can make bacteria cell wall break, and also directly can be combined with electronegative virus protein, forms double salt, make virally inactivated with DNA, RNA, apoprotein.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and sprouting the temperature of cultivating is 30 DEG C, and lywallzyme thus can be made to be in suitable hydrolysis temperature.Shitosan has bacteriostatic activity.Pyroligneous liquor can suppress the breeding of miscellaneous bacteria, has fungi-proofing, effect of insect protected, and in pyroligneous liquor, acetic acid content is higher, and solution, in acid, can dissolve shitosan, and make lysozyme and lywallzyme be in suitable sour environment, improve enzymolysis efficiency.
In the method for described promotion gomuti palm seed germination, the bottom of described culture dish is coated with photocatalyst, and the method for described promotion gomuti palm seed germination, also comprises:
With sealed membrane by after described culture dish sealing, when every day cultivates under illumination condition, with the bottom 5min of culture dish described in UV-irradiation, Continuous irradiation 90 days.Photocatalyst effectively can kill various bacteria, and the toxin that bacterium or fungi can be discharged decomposes and harmless treatment, also can prevent bottom culture dish mouldy further.
Embodiment 3
The invention provides a kind of method promoting gomuti palm seed germination, comprise the following steps:
Step one, by gomuti palm seed with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 12 DEG C, obtains the gomuti palm seed after lamination; Stratificated treatment can remove seed dormancy, promotes seed germination.
Step 2, the gomuti palm seed after described lamination to be loaded after in kraft paper bag, be placed in the drier that dry silica gel is housed, drying at room temperature 3h, obtain dry gomuti palm seed.Before sprouting is cultivated, some dehydration processing are carried out to seed, suitably can provide germination rate.
In the method for described promotion gomuti palm seed germination, described wet perlite is obtained after being mixed by the perlite of 1 weight portion and the sterile water of 1.7 weight portions.
In the method for described promotion gomuti palm seed germination, the gomuti palm seed after lamination described in described drier and the volume ratio of described dry silica gel are 1:100.
In the method for described promotion gomuti palm seed germination, before being mixed with described wet perlite by described gomuti palm seed in described step one, also comprise the step of described gomuti palm seed being carried out to sterilization, its concrete steps are:
Described gomuti palm seed is put into mass fraction be 0.1% liquor potassic permanganate soak 5min after, take out, with aseptic water washing 3 times.Sterilization is carried out to the gomuti palm seed gathered, the germ that seed carries can be reduced or eliminated.
In the method for described promotion gomuti palm seed germination, also comprise:
After sterilization being carried out to described gomuti palm seed in described step one, before described gomuti palm seed is mixed with described wet perlite, the gas-phase silica that volume is described wet perlite volume 25% is added in described wet perlite, wherein, the described gas-phase silica of half is absorbed with 50% carbendazol wettable powder, 250 times of liquid of deadweight 15 times, and second half described gas-phase silica is absorbed with 2.5% pleocidin suspension, 1250 times of liquid of deadweight 4 times.50% carbendazol wettable powder, the 250 times of liquid absorbed and 2.5% pleocidin suspension, 1250 times of liquid can slowly release by gas-phase silica, the impact of medicament on seed germination of higher concentration can be prevented, again can longer for seed germination provides suitable growing environment.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 3, carry out sterilization to the gomuti palm seed of drying, its concrete steps are:
First the gomuti palm seed of drying is put into volume fraction be 75% ethanol water soak 0.5min, take out, then put into mass fraction be 1% aqueous sodium hypochlorite solution soak 5min, take out, with aseptic water washing 3 times, obtain the gomuti palm seed of the drying after sterilization.Sterilization is carried out to the gomuti palm seed of drying, prevents dry gomuti palm seed from carrying more bacterium, cause and sprout the mold of seed in incubation, reduce germination rate.
In the method for described promotion gomuti palm seed germination, also comprise:
Step 4, the gomuti palm seed of the drying after the sterilization obtained in step 3 carried out to sprouting and cultivate, its concrete steps are:
Be that in the culture dish of 13cm, laying depth is after the perlite of 1.5cm equably at diameter, 105mL sterile water is added in culture dish, again the gomuti palm seed of the drying after 50 described sterilizations is placed in culture dish, mix with perlite, sealed by culture dish with sealed membrane afterwards, at 30 DEG C, every day first cultivates 12h under illumination condition, 12h is cultivated again, continuous culture 90 days under dark condition.
In the method for described promotion gomuti palm seed germination, in step 4, also comprise:
Lay perlite in described culture dish before, bottom even laying depth to described culture dish is the medical stone ball of 0.9cm, and in culture dish, pour 50% carbendazol wettable powder, 125 times of liquid of 12g into, on described medical stone ball, lay one deck filter paper identical with culture dish diameter afterwards, perlite is laid on described filter paper; Sprout in incubation, moisture can gradually to the bottom lamination of culture dish, cause bottom culture dish easily mouldy, medical stone ball is set bottom culture dish, medical stone ball energy attracts bacteria, and in culture dish, poured 50% carbendazol wettable powder, 125 times of liquid into, medical stone ball can absorb moisture and 50% carbendazol wettable powder, 125 times of liquid, and the medicament of absorption is slowly released, can ensure that the bottom of culture dish is in germ-free condition.
After the gomuti palm seed of the drying after sterilization is placed in culture dish, before mixing with perlite, the water-loss reducer being absorbed with deadweight 250 times of bactericide of 15g is added in perlite, mix with the gomuti palm seed of the drying after perlite and sterilization afterwards, the most backward perlitic surface uniform sprays the glycerine of 7g, wherein, described bactericide is made up of the component of following parts by weight: lysozyme 0.25 part, lywallzyme 0.4 part, shitosan 0.15 part, 2 parts, pyroligneous liquor and 25 parts, water.Lysozyme can make bacteria cell wall break, and also directly can be combined with electronegative virus protein, forms double salt, make virally inactivated with DNA, RNA, apoprotein.Lywallzyme can decompose fungal cell wall, and enzymolysis optimum temperature is 25-35 DEG C, and sprouting the temperature of cultivating is 30 DEG C, and lywallzyme thus can be made to be in suitable hydrolysis temperature.Shitosan has bacteriostatic activity.Pyroligneous liquor can suppress the breeding of miscellaneous bacteria, has fungi-proofing, effect of insect protected, and in pyroligneous liquor, acetic acid content is higher, and solution, in acid, can dissolve shitosan, and make lysozyme and lywallzyme be in suitable sour environment, improve enzymolysis efficiency.
In the method for described promotion gomuti palm seed germination, the bottom of described culture dish is coated with photocatalyst, and the method for described promotion gomuti palm seed germination, also comprises:
With sealed membrane by after described culture dish sealing, when every day cultivates under illumination condition, with the bottom 4min of culture dish described in UV-irradiation, Continuous irradiation 90 days.Photocatalyst effectively can kill various bacteria, and the toxin that bacterium or fungi can be discharged decomposes and harmless treatment, also can prevent bottom culture dish mouldy further.
Comparative example 1
1) collect seed, sterilize: in September, 2014, the gomuti palm fruit of maturation of gathering in Medicinal Plants of Guangxi garden, it is clean with the tap water of flowing again after seed is stripped out, dry in the shade 1 day in indoor, sieve gets 400, the hard seed of grey black, is divided into eight groups, and often the mass fraction of group 300mL is the liquor potassic permanganate soaking disinfection 5 minutes of 0.1%, use distilled water flushing 3 times after filtering out gomuti palm seed, carry out first time seed disinfection.
2) by four groups first time sterilization after gomuti palm seed respectively with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after four groups of laminations, and other four groups are not done Stratificated treatment.Wherein, wet perlite is mixed by the perlite of 1 weight portion and the water of 1.7 weight portions and obtains.
3) dehydration processing: the gomuti palm seed gomuti palm seed after four groups of laminations and four groups not being done Stratificated treatment mixes with 3000g silica gel to be placed in drier respectively and dewaters 3 hours.
4) sprout and cultivate: four groups of gomuti palm seeds successively after lamination and dehydration processing are taken out, is labeled as CJ1, CJ2, CJ3, CJ4; By four groups without Stratificated treatment, but the gomuti palm seed after dehydration processing takes out, and is labeled as CK1, CK2, CK3, CK4.Afterwards eight groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts is 75% respectively, then soak seed 5 minutes with the aqueous sodium hypochlorite solution that mass fraction is 1%, finally rinse 3 times with sterile distilled water, carry out second time seed disinfection.Often group gomuti palm seed after second time sterilization is evenly put in glass culture dish (diameter be 13cm glass culture dish in be paved with perlite in advance, through 120 DEG C of high-temperature sterilization process 20 minutes), sealed membrane sealing is placed in illumination box cultivates 90 days.Arrange illumination box 8:00 to 20:00 every day and carry out illumination, intensity of illumination is 1000 ~ 3000lx, and cultivation temperature is 30 DEG C; All the other times are dark, and cultivation temperature is 30 DEG C.
Account for often group with the gomuti palm seed grain number of radicle breakthrough seed coat after 90 days and be designated as seed germination rate for the percentage planting experimentally seed number, seed germination rate through CJ1, CJ2, CJ3, CJ4 of Stratificated treatment is respectively 68%, 78%, 85%, 73%, and average germination rate reaches 76%; And distinguish 2%, 6%, 0%, 2% without the seed germination rate of CK1, CK2, CK3, CK4 of Stratificated treatment, average germination rate reaches 2.5%.Illustrate and make the germination rate of gomuti palm seed improve 30.4 times through Stratificated treatment.
Comparative example 2
1) collect seed, sterilize: in September, 2014, the gomuti palm fruit of maturation of gathering in Medicinal Plants of Guangxi garden, it is clean with the tap water of flowing again after seed is stripped out, dry in the shade 1 day in indoor, sieve gets 400, the hard seed of grey black, be divided into eight groups, often the mass fraction of group 300mL is the liquor potassic permanganate soaking disinfection 5 minutes of 0.1%, filters out after gomuti palm seed with distilled water flushing 3 times.
2) by eight groups first time sterilization after gomuti palm seed respectively with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after eight groups of laminations.Wherein, wet perlite is mixed by the perlite of 1 weight portion and the water of 1.7 weight portions and obtains.
3) dehydration processing: the gomuti palm seed after eight groups of laminations is mixed with 3000g silica gel to be placed in drier respectively and dewaters 3 hours.
4) sprout and cultivate: four groups of gomuti palm seeds successively after lamination and dehydration processing are taken out, is labeled as CA1, CA2, CA3, CA4; Other four groups of gomuti palm seeds successively after lamination and dehydration processing are taken out, is labeled as CB1, CB2, CB3, CB4.Afterwards eight groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts is 75% respectively, then soak seed 5 minutes with the aqueous sodium hypochlorite solution that mass fraction is 1%, finally rinse 3 times with sterile distilled water.Often group gomuti palm seed after second time sterilization is evenly put in glass culture dish (diameter be 13cm glass culture dish in be paved with perlite in advance, through 120 DEG C of high-temperature sterilization process 20 minutes), sealed membrane sealing is placed in illumination box cultivates 90 days.CA1, CA2, CA3, CA4 group arranges illumination box 8:00 to 20:00 every day and carries out illumination, and intensity of illumination is 1000 ~ 3000lx, and cultivation temperature is 30 DEG C; All the other times are dark, and cultivation temperature is 30 DEG C.CB1, CB2, CB3, CB4 group arranges illumination box 8:00 to 20:00 every day and carries out illumination, and intensity of illumination is 1000 ~ 3000lx, and cultivation temperature is 30 DEG C; All the other times are dark, and cultivation temperature is 20 DEG C.
Account for often group with the gomuti palm seed grain number of radicle breakthrough seed coat after 90 days and be designated as seed germination rate for the percentage planting experimentally seed number, the seed germination rate of CA1, CA2, CA3, CA4 group is respectively 83%, 66%, 77%, 63%, and average germination rate reaches 72.25%; And the seed germination rate difference 8%, 0%, 3%, 1% of CB1, CB2, CB3, CB4 group, average germination rate reaches 3%.Illustrate that sprouting cultivation temperature in incubation maintains 30 DEG C always the germination rate of gomuti palm seed can be made to improve 24 times.
Comparative example 3
1) collect seed, sterilize: in September, 2014, the gomuti palm fruit of maturation of gathering in Medicinal Plants of Guangxi garden, it is clean with the tap water of flowing again after seed is stripped out, dry in the shade 1 day in indoor, sieve gets 400, the hard seed of grey black, be divided into eight groups, wherein four groups of mass fractions with 300mL are the liquor potassic permanganate soaking disinfection 5 minutes of 0.1%, filter out after gomuti palm seed with distilled water flushing 3 times.
2) by four groups first time sterilization after gomuti palm seed respectively with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10 DEG C, obtains the gomuti palm seed after four groups of laminations.Wherein, wet perlite is mixed by the perlite of 1 weight portion and the water of 1.7 weight portions and obtains.
3) dehydration processing: the gomuti palm seed after four groups of laminations is mixed with 3000g silica gel to be placed in drier respectively and dewaters 3 hours.
4) sprout and cultivate: four groups of gomuti palm seeds successively after lamination and dehydration processing are taken out, be labeled as CM1, CM2, CM3, CM4, afterwards these four groups of gomuti palm seeds are first soaked seed 0.5 minute with the ethanol that volume parts is 75% respectively, soak seed 5 minutes with the aqueous sodium hypochlorite solution that mass fraction is 1% again, finally rinse 3 times with sterile distilled water.Often group gomuti palm seed after second time sterilization is evenly put in glass culture dish (diameter be 13cm glass culture dish in be paved with perlite in advance, through 120 DEG C of high-temperature sterilization process 20 minutes), sealed membrane sealing is placed in illumination box cultivates 90 days.Other four group echos are CN1, CN2, CN3, CN4, to beginning to being left intact eventually, by often organize gomuti palm seed evenly to put in glass culture dish (diameter be 13cm glass culture dish in be paved with perlite in advance, through 120 DEG C of high-temperature sterilization process 20 minutes), sealed membrane sealing is placed in illumination box cultivates 90 days.The illumination box 8:00 to 20:00 every day that often arranges in eight groups carries out illumination, and intensity of illumination is 1000 ~ 3000lx, and cultivation temperature is 30 DEG C; All the other times are dark, and cultivation temperature is 30 DEG C.
Account for often group with the gomuti palm seed grain number of radicle breakthrough seed coat after 90 days and be designated as seed germination rate for the percentage planting experimentally seed number, seed germination rate through CM1, CM2, CM3, CM4 group of Stratificated treatment is respectively 56%, 66%, 58%, 76%, and average germination rate reaches 64%; And distinguish 2%, 6%, 0%, 2% without the seed germination rate of CN1, CN2, CN3, CN4 group of any process, average germination rate reaches 2.5%.Illustrate through first time sterilization, cross Stratificated treatment, second time sterilamp process after make the germination rate of gomuti palm seed improve 25.6 times.
Comparative example 4
Cultivate four groups of gomuti sugarpalm fruit seeds by the method shown in embodiment 2, be labeled as CP1, CP2, CP3, CP4, its seed germination rate is respectively 92%, 95%, 90%, 97%, and average germination rate reaches 93.5%; The seed germination rate of CJ1, CJ2, CJ3, CJ4 in comparative example 1 is respectively 68%, 78%, 85%, 73%, and average germination rate reaches 76%, illustrates that the present invention can improve the germination rate of seed further.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (9)
1. promote a method for gomuti palm seed germination, it is characterized in that, comprise the following steps:
Step one, by gomuti palm seed with wet perlite by volume for 1:3 mixes, after sealing, lamination 90 days at 10-15 DEG C, obtains the gomuti palm seed after lamination;
Step 2, the gomuti palm seed after described lamination to be loaded after in kraft paper bag, be placed in the drier that dry silica gel is housed, drying at room temperature 3h, obtain dry gomuti palm seed.
2. the method promoting gomuti palm seed germination as claimed in claim 1, is characterized in that, described wet perlite is obtained after being mixed by the perlite of 1 weight portion and the sterile water of 1.4-2 weight portion.
3. the method promoting gomuti palm seed germination as claimed in claim 1, it is characterized in that, the gomuti palm seed after lamination described in described drier and the volume ratio of described dry silica gel are 1:100.
4. the method promoting gomuti palm seed germination as claimed in claim 1, it is characterized in that, before being mixed with described wet perlite by described gomuti palm seed in described step one, also comprise the step of described gomuti palm seed being carried out to sterilization, its concrete steps are:
Described gomuti palm seed is put into mass fraction be 0.1% liquor potassic permanganate soak 5min after, take out, with aseptic water washing 2-3 time.
5. the method promoting gomuti palm seed germination as claimed in claim 4, is characterized in that, also comprise:
After sterilization being carried out to described gomuti palm seed in described step one, before described gomuti palm seed is mixed with described wet perlite, the gas-phase silica that volume is described wet perlite volume 20-30% is added in described wet perlite, wherein, the described gas-phase silica of half is absorbed with deadweight 10-20 50% carbendazol wettable powder 200-300 times liquid doubly, and second half described gas-phase silica is absorbed with deadweight 3-5 2.5% pleocidin suspension 1000-1500 times liquid doubly.
6. the method promoting gomuti palm seed germination as claimed in claim 1, is characterized in that, also comprise:
Step 3, carry out sterilization to the gomuti palm seed of drying, its concrete steps are:
First the gomuti palm seed of drying is put into volume fraction be 75% ethanol water soak 0.5min, take out, then put into mass fraction be 1% aqueous sodium hypochlorite solution soak 5min, take out, with aseptic water washing 3 times, obtain the gomuti palm seed of the drying after sterilization.
7. the method promoting gomuti palm seed germination as claimed in claim 6, is characterized in that, also comprise:
Step 4, the gomuti palm seed of the drying after the sterilization obtained in step 3 carried out to sprouting and cultivate, its concrete steps are:
Be that in the culture dish of 13cm, laying depth is after the perlite of 1-2cm equably at diameter, 100-110mL sterile water is added in culture dish, again the gomuti palm seed of the drying after 50 described sterilizations is placed in culture dish, mix with perlite, sealed by culture dish with sealed membrane afterwards, at 30 DEG C, every day first cultivates 12h under illumination condition, 12h is cultivated again, continuous culture 90 days under dark condition.
8. the method promoting gomuti palm seed germination as claimed in claim 7, is characterized in that, in step 4, also comprise:
Lay perlite in culture dish before, bottom even laying depth to culture dish is the medical stone ball of 0.8-1cm, and in culture dish, pour the 50% carbendazol wettable powder 100-150 times liquid of 10-15g into, on described medical stone ball, lay one deck filter paper identical with culture dish diameter afterwards, perlite is laid on described filter paper;
After the gomuti palm seed of the drying after sterilization is placed in culture dish, before mixing with perlite, the water-loss reducer being absorbed with deadweight 200-300 times of bactericide of 10-20g is added in perlite, mix with the gomuti palm seed of the drying after perlite and sterilization afterwards, the most backward perlitic surface uniform sprays the glycerine of 5-10g, wherein, described bactericide is made up of the component of following parts by weight: lysozyme 0.2-0.3 part, lywallzyme 0.3-0.5 part, shitosan 0.1-0.2 part, pyroligneous liquor 1-3 part and water 20-30 part.
9. the method promoting gomuti palm seed germination as claimed in claim 8, it is characterized in that, the bottom of described culture dish is coated with photocatalyst, and the method for described promotion gomuti palm seed germination, also comprises:
With sealed membrane by after described culture dish sealing, when every day cultivates under illumination condition, with the bottom 3-5min of culture dish described in UV-irradiation, Continuous irradiation 90 days.
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