CN106831123A - A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof - Google Patents
A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof Download PDFInfo
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- CN106831123A CN106831123A CN201710044749.XA CN201710044749A CN106831123A CN 106831123 A CN106831123 A CN 106831123A CN 201710044749 A CN201710044749 A CN 201710044749A CN 106831123 A CN106831123 A CN 106831123A
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- convex rod
- concave convex
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- keeping fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The invention discloses a kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof.Described composite biomass water keeping fertilizer is made up of bacteria cellulose and concave convex rod, and bacteria cellulose parcel is grown in the surface of concave convex rod, and the mass ratio of bacteria cellulose and concave convex rod is 5:1~1:5.The present invention disperses and is immersed in bio-fermented liquid by by attapulgite, cultivation and fermentation, makes it compound with bacteria cellulose, isolates and purifies tunning and bacteria cellulose/concave convex rod composite biomass water keeping fertilizer is obtained.Biomass water keeping fertilizer of the invention is pollution-free, and noresidue, environmental protection is easy to use, can play good water storage and release water, keeps soil from packing together, and promotes the effect such as growth of microorganism, preparation process is simple, mild condition, it is easy to which extensive standardization is produced and applied.
Description
Technical field
The invention belongs to Biocomposite material field, it is related to a kind of bacteria cellulose/concave convex rod composite biomass water-retention fertilizer
Material and preparation method thereof.
Background technology
Environment-friendly highly efficient water keeping fertilizer is directed to the domain requirements such as agricultural, gardens, gardening and largely uses water conservation, water saving material
Material, and the composite of Multifunction always developed, because its unique water-absorbing-retaining holds the performances such as fertilizer, in control of desert and
The aspects such as farmland drought resisting are widely used.
Attapulgite is that a kind of crystalloid is hydrated alumina magnesia silicate, and with unique layer chain structure feature, crystal is in
Needle-like, threadiness or fiber collection shape, its preferable chemical molecular formula is：Mg5Si8O20(OH)2(OH2)4·4H2O.Attapulgite
Clay has special pore passage structure, interfacial property and larger specific surface area.Chinese patent CN105174278A is disclosed
A kind of preparation method of modified attapulgite and the method for preparing biomass water keeping fertilizer as primary raw material with modified attapulgite.It is concavo-convex
Rod soil have preferable cation replacement property, adsorptivity, water imbibition, cohesiveness the features such as, can with the materialization property of improved soil,
Improve the hydrothermal condition of soil.Meanwhile, attapulgite contains mineral nutrient element higher, using the teaching of the invention it is possible to provide trace element.It is concavo-convex
Although rod is with good water suction property, but its bulk density is difficult to form detritus layer greatly, and water retaining function is not all right, and itself is not
Fertilizer.Bacteria cellulose is close with common plant cellulose in chemical composition and molecular structure, is connected by β-Isosorbide-5-Nitrae glucose
The direct-connected macromolecular for connecing.Bacteria cellulose high-purity, high polymerization degree, also release control water function, while fine with good suction
Dimension element itself can form the water conservation humus layer with fine air permeability as organic nutriment of soil.
The content of the invention
It is the invention provides a kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, concave convex rod and bacterium is fine
Dimension element is compound to be made biomass water keeping fertilizer, can improved soil, its water conservation is breathed freely, keep soil from packing together, and farming is provided
The inorganic nutriment in organic and part required for thing growth.
Technical scheme is as follows：
A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, is made up of bacteria cellulose and concave convex rod, described
Bacteria cellulose parcel be grown in the surface of concave convex rod, described bacteria cellulose and the mass ratio of concave convex rod are 5:1~1:
5。
Preferably, the mass ratio of described bacteria cellulose and concave convex rod is 3:1~1:1.
The present invention also provides the preparation method of above-mentioned bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, by inciting somebody to action
Attapulgite is obtained in being added to the fermentation medium of bacteria cellulose, comprises the following steps：
Step 1, the attapulgite clay raw ore by attapulgite content not less than 98% is crushed, and crosses 100~300 mesh sieves,
Concave convex rod powder is obtained, concave convex rod powder is immersed in hydrochloric acid；
Step 2, by 5%~10% inoculum concentration, acetobacter xylinum seed liquor is inoculated into fermentation medium, is subsequently adding
It is 1 with the mass volume ratio of acetobacter xylinum seed liquor:1~4:1 concave convex rod powder, fermented and cultured obtains bacteria cellulose/recessed
Convex rod compound；
Step 3, after bacteria cellulose/concave convex rod compound is cleaned, sterilization treatment at 120 DEG C is immersed in 4% hydrogen-oxygen
Change in sodium and 0.1% mixed solution of hydrogen peroxide, 8~12h is kept at 100 DEG C, add NaAc_HAc buffer solution to neutralize
Excessive NaOH, is washed to pH to neutrality, is drying to obtain bacteria cellulose/concave convex rod composite biomass water keeping fertilizer.
Preferably, in step 2, described acetobacter xylinum is Acetobacter xylinum NUST4 bacterium.
Preferably, in step 2, the inoculum concentration of acetobacter xylinum seed liquor is 7%.
Preferably, in step 2, concave convex rod powder is 2 with the mass volume ratio of acetobacter xylinum seed liquor:1.
Preferably, described fermentation medium is：Glucose 4~7%, sucrose 1~3%, corn pulp 1.5~3.5%, sulphur
Sour ammonium 0.2~0.4%, potassium dihydrogen phosphate 0.1~0.3%, magnesium sulfate 0.02~0.04%, calcium lactate 0.01~0.03%, just
Beginning pH 6.0.
Biomass water keeping fertilizer of the invention has good water retention property, can absorb tens times of its own weight so that on
Thousand times of water, can not only increase soil moisture content, and prevent erosion.Meanwhile, biomass water keeping fertilizer energy of the invention
Enough promote the formation of soil agreegate, strengthen soil permeability, reduce plant death rate, improve soil fertilizer ability, protect it
Water is breathed freely, and prevents hardened, and the inorganic nutriment in organic and part required for providing crop growth.Bacteria cellulose/concave convex rod
Composite biomass water keeping fertilizer is used 3 years or so, and attapulgite turns into soil soil property part, and bacteria cellulose also can conduct
Soil nutrient forms humus layer by natural degradation, effectively prevents soil hardening and provides fertility, is the free of contamination, Neng Gougai of one kind
The biomass water keeping fertilizer of good soil.Preparation method environmental protection of the invention, the biological quality guarantee prepared by microorganism means
Liquid manure material process is simple, mild condition, it is easy to extensive standardization production and application.
Brief description of the drawings
Fig. 1 is the TEM figures of bacteria cellulose (a) and bacteria cellulose/concave convex rod composite biomass water keeping fertilizer (b).
Specific embodiment
With reference to embodiment and accompanying drawing, the invention will be further described.
A kind of preparation method of the bacteria cellulose of embodiment 1/concave convex rod composite biomass water keeping fertilizer, step is as follows：
It is prepared by step 1, concave convex rod powder：The attapulgite clay raw ore for selecting attapulgite content to be 99% is ground into logical
200 mesh sieves are crossed, the concave convex rod powder of gained is immersed in 1% hydrochloric acid solution 1h, and be washed with deionized, by 121 DEG C
It is standby after sterilizing 20min.
Step 2, seed culture：Configuration seed culture medium 50mL：Glucose 5%, corn pulp 0.6%, ammonium sulfate 0.3%,
Dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.05%, peptone 0.5%, DEG C sterilizing of sodium carboxymethylcellulose 0.05%, pH5.2,121
20min。
1g Acetobacter xylinum NUST4 strains are put into seed culture medium to be cultivated, 29 DEG C of concussion and cultivates
24h, shaking speed is 180r/min.
Step 3, fermented and cultured：Configuration fermentation medium 500mL：Glucose 3%, sucrose 2.5%, corn pulp 2%, sulfuric acid
Ammonium 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, calcium lactate 0.02%, initial pH6.0,121 DEG C of sterilizing 20min.
Seed liquor is accessed with 5% inoculum concentration (25mL), and it is 2 to add with seed liquor mass volume ratio：1 attapulgite
Last (5g), shakes up, and is cultivated 5 days under 29 DEG C of constant temperatures, bacteria cellulose is successfully grown on concave convex rod, obtains bacterium fine
Dimension element/concave convex rod compound.
Step 4, isolates and purifies：By bacteria cellulose/concave convex rod compound washed several times with water, enter in 120 DEG C of autoclave
Row sterilization processing.Again with after 4% NaOH and 0.1% hydroxide hydrogen mixed solution immersion treatment, heating water bath to 100
DEG C, keep 10h.With the NaOH in NaAc_HAc buffer solution with excess, it is neutrality to be washed to pH, after natural drying,
Bacteria cellulose/concave convex rod composite biomass water keeping fertilizer is obtained, 10.5g is weighed as.
Embodiment 2
A kind of preparation method of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, step is as follows：
It is prepared by step 1, concave convex rod powder：The attapulgite clay raw ore for selecting attapulgite content to be 98% is ground into logical
100 mesh sieves are crossed, the concave convex rod powder of gained is immersed in 1% hydrochloric acid solution 1h, and be washed with deionized, by 121 DEG C
It is standby after sterilizing 20min.
Step 2, seed culture：Configuration seed culture medium 50mL：Glucose 5%, corn pulp 0.6%, ammonium sulfate 0.3%,
Dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.05%, peptone 0.5%, DEG C sterilizing of sodium carboxymethylcellulose 0.05%, pH5.2,121
20min。
1g Acetobacter xylinum NUST4 strains are put into seed culture medium to be cultivated, 29 DEG C of concussion and cultivates
24h, shaking speed is 180r/min.
Step 3, fermented and cultured：Configuration fermentation medium 500mL：Glucose 3%, sucrose 2.5%, corn pulp 2%, sulfuric acid
Ammonium 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, calcium lactate 0.02%, initial pH6.0,121 DEG C of sterilizing 20min.
Seed liquor is accessed with 7% inoculum concentration (35mL), and it is 1 to add with seed liquor mass volume ratio：1 attapulgite
Last (35g), shakes up, and is cultivated 5 days under 29 DEG C of constant temperatures, bacteria cellulose is successfully grown on concave convex rod, obtains bacterium
Cellulose/concave convex rod compound.
Step 4, isolates and purifies：By bacteria cellulose/concave convex rod compound washed several times with water, enter in 120 DEG C of autoclave
Row sterilization processing.Again with after 4% NaOH and 0.1% hydroxide hydrogen mixed solution immersion treatment, heating water bath to 100
DEG C, keep 8h.With the NaOH in NaAc_HAc buffer solution with excess, it is neutrality to be washed to pH, after natural drying,
Bacteria cellulose/concave convex rod composite biomass water keeping fertilizer is obtained, 40.1g is weighed as.
Embodiment 3
A kind of preparation method of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, step is as follows：
It is prepared by step 1, concave convex rod powder：The attapulgite clay raw ore for selecting attapulgite content to be 99% is ground into logical
300 mesh sieves are crossed, the concave convex rod powder of gained is immersed in 1% hydrochloric acid solution 1h, and be washed with deionized, by 121 DEG C
It is standby after sterilizing 20min.
Step 2, seed culture：Configuration seed culture medium 50mL：Glucose 5%, corn pulp 0.6%, ammonium sulfate 0.3%,
Dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.05%, peptone 0.5%, DEG C sterilizing of sodium carboxymethylcellulose 0.05%, pH5.2,121
20min。
1g Acetobacter xylinum NUST4 strains are put into seed culture medium to be cultivated, 29 DEG C of concussion and cultivates
24h, shaking speed is 180r/min.
Step 3, fermented and cultured：Configuration fermentation medium 500mL：Glucose 3%, sucrose 2.5%, corn pulp 2%, sulfuric acid
Ammonium 0.3%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.05%, calcium lactate 0.02%, initial pH6.0,121 DEG C of sterilizing 20min.
Seed liquor is accessed with 10% inoculum concentration (50mL), and it is 4 to add with seed liquor mass volume ratio：1 concave convex rod
Powder (200g), shakes up, and is cultivated 5 days under 29 DEG C of constant temperatures, bacteria cellulose is successfully grown on concave convex rod, obtains thin
Fungin/concave convex rod compound.
Step 4, isolates and purifies：By bacteria cellulose/concave convex rod compound washed several times with water, enter in 120 DEG C of autoclave
Row sterilization processing.Again with after 4% NaOH and 0.1% hydroxide hydrogen mixed solution immersion treatment, heating water bath to 100
DEG C, keep 12h.With the NaOH in NaAc_HAc buffer solution with excess, it is neutrality to be washed to pH, after natural drying,
Bacteria cellulose/concave convex rod composite biomass water keeping fertilizer is obtained, 205.7g is weighed as.
Embodiment 4
The application effect of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer
Experiment material：Cucumber seedling seed
Experimental technique：
A, test group：Implement water keeping fertilizer prepared by the above method：
If two experimental groups, I group 2g/ basins；II group 3g/ basins, each treatment is repeated 3 times.
B, control group：Individually implement concave convex rod and bacteria cellulose：
If two experimental groups, the mesh molecular sieve of mistake 200 of III group 2g/ basins crushes attapulgite；IV group 2g/ basins do not add recessed
The cotton-shaped fermented bacterial cellulose of convex rod.
Experimental procedure：These fertilizer or material are sufficiently mixed with soil prior to seeding, then are placed 3.Do not have basin to sow
Cucumber seeds 20, after planting water 300mL/ basins at once.
Do not watered in 20 days for emerging.Emergence rate (cucumber seeds emerge quantity and total cucumber seeds ratio of number) and its
Upgrowth situation is as shown in table 1.
The cucumber emergence rate of each experimental group of table 1
| Experimental group | Emergence rate | Upgrowth situation |
| I | 78% | Seedling leaves are light green, well-grown |
| II | 88% | Seedling leaves are light green, well-grown |
| III | 5% | Seedling is substantially withered |
| IV | 30% | Seedling leaves turn yellow, and growth is general |
As it can be seen from table 1 the content that I and II group profiles increase water keeping fertilizer in soil is conducive to the life of cucumber seedling
It is long；I and IV illustrate that bacteria cellulose can play fertilizer effect, but cannot play Water-saving effect；I and III illustrate recessed
Convex rod is in itself and without the effect for providing plant growth fertilizer.
Embodiment 5
The Water-saving effect of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer
Experiment material：Basin fills soil
Experimental technique：
A, test group：Implement water keeping fertilizer prepared by the above method：
If two experimental groups, I group 2g/ basins；II group 3g/ basins, each treatment is repeated 3 times
B, control group：Individually implement concave convex rod and bacteria cellulose：
If two experimental groups, the mesh molecular sieve of mistake 200 of III group 2g/ basins crushes attapulgite；IV group 2g/ basins do not add recessed
The cotton-shaped fermented bacterial cellulose of convex rod.
C, blank group：V groups are left intact.
Experimental procedure, pours into 300mL water in each basin.
The soil moisture content by each experimental group after different time sections is detected, as a result as shown in table 2.
The soil moisture content of each experimental group of table 2
From table 2 it can be seen that from I and II, the appropriate content for increasing water keeping fertilizer in soil is conducive to soil water-retaining；
From I, III and V, concave convex rod has certain soaking effect in itself, but without good Water-saving effect；Can by II and IV
Know, although bacterial fibers have good soaking effect, but without good Water-saving effect.In summary it can be seen, this water conservation
Fertilizer has extremely good Water-saving effect relative to each control group.
Claims (7)
1. a kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer, it is characterised in that by bacteria cellulose and concave convex rod
Composition, described bacteria cellulose parcel is grown in the surface of concave convex rod, described bacteria cellulose and the mass ratio of concave convex rod
It is 5:1~1:5.
2. bacteria cellulose according to claim 1/concave convex rod composite biomass water keeping fertilizer, it is characterised in that described
Bacteria cellulose and concave convex rod mass ratio be 3:1~1:1.
3. the preparation method of bacteria cellulose according to claim 1 and 2/concave convex rod composite biomass water keeping fertilizer, its
It is characterised by, comprises the following steps：
Step 1, the attapulgite clay raw ore by attapulgite content not less than 98% is crushed, and crosses 100~300 mesh sieves, is obtained
Concave convex rod powder, concave convex rod powder is immersed in hydrochloric acid；
Step 2, by 5%~10% inoculum concentration, acetobacter xylinum seed liquor is inoculated into fermentation medium, is subsequently adding and wood
The mass volume ratio of acetobacter seed liquor is 1:1~4:1 concave convex rod powder, fermented and cultured obtains bacteria cellulose/concave convex rod
Compound；
Step 3, after bacteria cellulose/concave convex rod compound is cleaned, sterilization treatment at 120 DEG C is immersed in 4% NaOH
In 0.1% mixed solution of hydrogen peroxide, 8~12h is kept at 100 DEG C, it is in addition NaAc_HAc buffer solution and excessive
NaOH, be washed to pH to neutrality, be drying to obtain bacteria cellulose/concave convex rod composite biomass water keeping fertilizer.
4. preparation method according to claim 3, it is characterised in that in step 2, described acetobacter xylinum is
Acetobacter xylinum NUST4 bacterium.
5. preparation method according to claim 3, it is characterised in that in step 2, the inoculum concentration of acetobacter xylinum seed liquor is
7%.
6. preparation method according to claim 3, it is characterised in that in step 2, concave convex rod powder and acetobacter xylinum seed
The mass volume ratio of liquid is 2:1.
7. preparation method according to claim 3, it is characterised in that described fermentation medium is：Glucose 4~7%,
Sucrose 1~3%, corn pulp 1.5~3.5%, ammonium sulfate 0.2~0.4%, potassium dihydrogen phosphate 0.1~0.3%, magnesium sulfate 0.02
~0.04%, calcium lactate 0.01~0.03%, initial pH 6.0.
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Cited By (5)
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| CN109535267A (en) * | 2018-10-25 | 2019-03-29 | 珠海市麦可美生物技术有限公司 | A kind of preparation method of the maltodextrin of low DE value |
| CN110218112A (en) * | 2019-05-31 | 2019-09-10 | 王红玲 | A kind of biological compound fertilizer and preparation method thereof using waste straw preparation |
| WO2020058183A1 (en) * | 2018-09-21 | 2020-03-26 | Jassen - Kunststoffzentrum Gmbh - Apparatebau, Zuschnitte Und Formung | Fertilizer carrier, method for producing a fertillizer, and fertillizer |
| TWI692524B (en) * | 2018-12-28 | 2020-05-01 | 嬌朋生技股份有限公司 | Cultivated material composition |
| CN111533613A (en) * | 2020-03-25 | 2020-08-14 | 东华大学 | Nano-cellulose gel-based water-retaining slow-release fertilizer and preparation method thereof |
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| CN111533613B (en) * | 2020-03-25 | 2023-02-28 | 东华大学 | Nano-cellulose gel-based water-retaining slow-release fertilizer and preparation method thereof |
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Application publication date: 20170613 |