CN104911230A - Bacterial cellulose in-situ fermentation production method - Google Patents
Bacterial cellulose in-situ fermentation production method Download PDFInfo
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- CN104911230A CN104911230A CN201510391158.0A CN201510391158A CN104911230A CN 104911230 A CN104911230 A CN 104911230A CN 201510391158 A CN201510391158 A CN 201510391158A CN 104911230 A CN104911230 A CN 104911230A
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Abstract
The invention discloses a bacterial cellulose in-situ fermentation production method. Non-metallic mineral inorganic gel is added into a bacterial cellulose fermentation medium, bacterial cellulose strains are added, and bacterial cellulose products are obtained through separation after a certain time of fermentation. According to the bacterial cellulose in-situ fermentation production method, bacterial cellulose is produced through the non-metallic mineral inorganic gel and accordingly the high value of application of a non-metallic mine is implemented, the water absorption performance or the water retaining capacity of the bacterial cellulose is improved due to the adding of the inorganic gel, and the technological process and the time are greatly shortened due to the fact that the fermentation and the bacterial cellulose modification are achieved in the same system due to the in-situ fermentation mode.
Description
Technical field
The present invention relates to the preparation method of bacteria cellulose, be specifically related to a kind of nonmetalliferous ore inorganic gel that adds and carry out the method that in-situ fermentation prepares bacteria cellulose, belong to biological chemical field.
Background technology
Bacteria cellulose (Bacterial cellulose) is the class biological polymeric compound synthesized by some bacterium.Different from plant cellulose, bacteria cellulose is not the constituent of cell walls, but bacterial secretory is to the product outside born of the same parents, and it is in independently filamentary fibers form, and the plant cellulose such as xylogen, the hemicellulose impurity that undopes.Compared with plant cellulose, bacteria cellulose has physico-chemical property and the mechanical property of many uniquenesses, includes ultra-fine property (nano level), high purity, high-crystallinity; Exist with Single Fiber form, there is consistent molecular orientation; High Young's modulus, tensile strength and shape maintains ability; Extremely strong water-holding power; Higher biological fitness; Shape and performance controllable etc.Due to physico-chemical property and the mechanical property of its uniqueness, bacteria cellulose is widely used in the fields such as environmental protection, papermaking, food, polymer, medical treatment.
In order to improve the water absorbing properties of bacteria cellulose further, be necessary that appendix is expected to increase the group of its water absorbing properties in bacteria cellulose.Bacteria cellulose in-situ fermentation (
in situfermentation) refer to before fermentation, add different compounds, then carry out bacteria cellulose fermentation, while fermentation, compound can realize the in-situ modified of product by appendix on bacteria cellulose.In order to realize bacteria cellulose water suction and the raising of water retention property, in-situ fermentation technology is used to add xenobiotic substrates in the medium.In numerous substrates, inorganic gel becomes the desirable xenobiotic substrates of super water-absorbent energy bacteria cellulose fermentation, and its advantage has two aspects, and one is that inorganic gel has the water-soluble swollen physicals of chance, and it is expected the water absorbing properties strengthening bacteria cellulose; Two is that inorganic gel itself is nontoxic, compatible with general aqueous ferment environment, does not affect bacteria cellulose fermenting process.
Summary of the invention
The object of the invention is: provide a kind of in-situ fermentation to prepare the method for bacteria cellulose, carry out in-situ fermentation by interpolation nonmetalliferous ore inorganic gel and prepare bacteria cellulose, nonmetalliferous ore raw material sources are extensive, cheap, use it to prepare inorganic gel and industrially have broad prospects.
The present invention is achieved by the following technical programs: first add nonmetalliferous ore inorganic gel in fermentation medium for bacterial cellulose, then adds product bacterial cellulose strain, after certain hour fermentation, is separated and obtains bacterial cellulose product.
Wherein, described nonmetalliferous ore inorganic gel comprises bentonite inorganic gel, attapulgite inorganic gel; In substratum, inorganic gel addition is 0.5-5 g/L.
Wherein, fermentation medium for bacterial cellulose is HS substratum or ligno-cellulose hydrolysate; Product bacterial cellulose strain is acetobacter xylinum.
Wherein, fermentation mode comprises dynamic fermentation and static fermentation, and leavening temperature is 20-35
oc; Inoculum size is 1-15% (v/v).
Wherein, bacteria cellulose separation method is centrifugal or filters.
The invention has the beneficial effects as follows: use nonmetalliferous ore inorganic gel to prepare bacteria cellulose, realize the high-valued application of nonmetalliferous ore, the water absorbing properties adding raising bacteria cellulose of inorganic gel or water retention capacity, in-situ fermentation pattern makes fermentation and bacteria cellulose modification complete in same system, shortened process and the time greatly.
Embodiment
Be described further technical scheme of the present invention below in conjunction with specific embodiment, embodiment is not limitation of the present invention.
embodiment 1:0.5 g/Lbentonite inorganic gel adds in HS substratum and forms in-situ fermentation system, and to add after acetobacter xylinum static fermentation in system 20 days, leavening temperature is 20
oc, inoculum size is 1% (v/v); After fermentation ends, filter and obtain bacterial cellulose product, products obtained therefrom water holding capacity improves 47.0% than not adding inorganic gel group.
embodiment 2:5 g/Lattapulgite inorganic gel adds in Corncob hydrolysate substratum and forms in-situ fermentation system, and to add after acetobacter xylinum dynamic fermentation in system one week, leavening temperature is 25
oc, inoculum size is 15% (v/v); After fermentation ends, centrifugal acquisition bacterial cellulose product, products obtained therefrom water retention capacity improves 20.0% than not adding inorganic gel group.
embodiment 3:2.5 g/Lattapulgite inorganic gel adds in HS substratum and forms in-situ fermentation system, and to add after acetobacter xylinum dynamic fermentation in system 4 days, leavening temperature is 30
oc, inoculum size is 8% (v/v); After fermentation ends, centrifugal acquisition bacterial cellulose product, products obtained therefrom water holding capacity improves 25.0% than not adding inorganic gel group.
embodiment 4:1 g/Lbentonite inorganic gel adds in bagasse hydrolyzed solution substratum and forms in-situ fermentation system, and to add after acetobacter xylinum static fermentation in system two weeks, leavening temperature is 35
oc, inoculum size is 10% (v/v); After fermentation ends, filter and obtain bacterial cellulose product, products obtained therefrom water retention capacity improves 15.0% than not adding inorganic gel group.
Claims (6)
1. in-situ fermentation prepares the method for bacteria cellulose, it is characterized in that: first add nonmetalliferous ore inorganic gel in fermentation medium for bacterial cellulose, then adds product bacterial cellulose strain, after certain hour fermentation, is separated and obtains bacterial cellulose product.
2. in-situ fermentation according to claim 1 prepares the method for bacteria cellulose, it is characterized in that: described nonmetalliferous ore inorganic gel comprises bentonite inorganic gel, attapulgite inorganic gel; In substratum, inorganic gel addition is 0.5-5 g/L.
3. in-situ fermentation according to claim 1 prepares the method for bacteria cellulose, it is characterized in that: fermentation medium for bacterial cellulose is HS substratum or ligno-cellulose hydrolysate.
4. in-situ fermentation according to claim 1 prepares the method for bacteria cellulose, it is characterized in that: product bacterial cellulose strain is acetobacter xylinum.
5. in-situ fermentation according to claim 1 prepares the method for bacteria cellulose, it is characterized in that: fermentation mode comprises dynamic fermentation and static fermentation; Leavening temperature is 20-35
oc, inoculum size is 1-15% (v/v), and fermentation time is 4-20 days.
6. in-situ fermentation according to claim 1 prepares the method for bacteria cellulose, it is characterized in that: bacteria cellulose separation method is centrifugal or filters.
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Cited By (6)
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| CN106831123A (en) * | 2017-01-19 | 2017-06-13 | 南京理工大学 | A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof |
| CN107044054A (en) * | 2017-04-12 | 2017-08-15 | 江南大学 | A kind of bacteria cellulose in situ/eider down insulation material and preparation method thereof |
| CN107698805A (en) * | 2016-08-09 | 2018-02-16 | 丁玉琴 | A kind of defervescence plaster used preparation method with compound water congealing glued membrane |
| CN109248663A (en) * | 2018-09-28 | 2019-01-22 | 芜湖市鹏磊新材料有限公司 | A kind of preparation method of use in waste water treatment bentonite adsorption material |
| CN110563886A (en) * | 2018-06-06 | 2019-12-13 | 中国科学院广州能源研究所 | bacterial cellulose-based super absorbent material and preparation method thereof |
| CN112695009A (en) * | 2021-01-29 | 2021-04-23 | 广东药科大学 | Method for preparing bacterial cellulose by in-situ fermentation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107698805A (en) * | 2016-08-09 | 2018-02-16 | 丁玉琴 | A kind of defervescence plaster used preparation method with compound water congealing glued membrane |
| CN106831123A (en) * | 2017-01-19 | 2017-06-13 | 南京理工大学 | A kind of bacteria cellulose/concave convex rod composite biomass water keeping fertilizer and preparation method thereof |
| CN107044054A (en) * | 2017-04-12 | 2017-08-15 | 江南大学 | A kind of bacteria cellulose in situ/eider down insulation material and preparation method thereof |
| CN110563886A (en) * | 2018-06-06 | 2019-12-13 | 中国科学院广州能源研究所 | bacterial cellulose-based super absorbent material and preparation method thereof |
| CN109248663A (en) * | 2018-09-28 | 2019-01-22 | 芜湖市鹏磊新材料有限公司 | A kind of preparation method of use in waste water treatment bentonite adsorption material |
| CN112695009A (en) * | 2021-01-29 | 2021-04-23 | 广东药科大学 | Method for preparing bacterial cellulose by in-situ fermentation |
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Address after: 211700 Dongfang Road, Xuyi County, Huaian, Jiangsu Province, No. 3 Patentee after: Xuyi Aotu energy and environmental protection materials R & D Center Patentee after: GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES Address before: 211700 Dongfang Road, Xuyi County, Huaian, Jiangsu Province, No. 3 Patentee before: XUYI ATTAPULGITE RESEARCH & DEVELOPMENT CENTER OF GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES Patentee before: GUANGZHOU INSTITUTE OF ENERGY CONVERSION, CHINESE ACADEMY OF SCIENCES |