CN106613093A - Premna puberula cutting propagation method - Google Patents
Premna puberula cutting propagation method Download PDFInfo
- Publication number
- CN106613093A CN106613093A CN201610887084.4A CN201610887084A CN106613093A CN 106613093 A CN106613093 A CN 106613093A CN 201610887084 A CN201610887084 A CN 201610887084A CN 106613093 A CN106613093 A CN 106613093A
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- CN
- China
- Prior art keywords
- cuttage
- bavin
- cuttings
- body odour
- cutting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a premna puberula cutting propagation method. The method includes the steps that a premna puberula annual branch growing under the moderate shading condition is cut in the bud dormancy period to serve as a cutting material, a trimmed cutting shoot is soaked with a solution of 6-BA 300 mg.L<1>+NAA 200 mg.L<1> for cutting, the cutting shoot is transplanted after surviving, and a newborn plant can be obtained. The proliferation problem of seedlings of premna puberula in the large-scale artificial planting is effectively solved, and a good foundation is laid for achieving sustainable use of resources and industrialized development.
Description
Technical field
The present invention relates to be related to plant asexual reproduction method, and in particular to a kind of body odour bavin cuttage breeding method.
Background technology
Body odour bavin（Premna puberulaPamp.）, also known as angle's Japanese Premna, puberulent premna leaf or root etc., belong to Verenaceae beans
Rotten bavin belongs to perennial machaka, is distributed mainly on China Guizhou, Gansu, Southern Shaanxi, Hubei, Hunan, Sichuan and Yunnan etc.
Ground, is a kind of medicinal and edible plants in being grown on the hillside jungle of the m of height above sea level 700～1800.The root of body odour bavin, stem, Ye Junke
It is used as medicine, has the effects such as clearing heat and promoting diuresis, removing toxicity for detumescence, menstruation regulating establishing-Yang.The blade of body odour bavin contains abundant pectin, carbohydrate, albumen
Matter, amino acid, vitamin and mineral element.With " angle's beans of the green kingfisher of color and luster made by it, delicious flavour and energy clearing away heat and removing summer
It is rotten " there is very high edibility, like among the people enjoying.In addition pursuit of the today's society people to health food so that fox
" angle's bean curd " of smelly bavin leaf production has wide market prospects.
Under natural conditions, body odour bavin is although distributed more widely but does not concentrate, and population quantity is few, yields positive results less and seed sprouts tired
Difficulty, causes its wild resource day by day deficient.Therefore, it is the raw materials requirement that meets body odour bavin industrialization development, its artificial growth gesture
Must go, and seedling breeding is then the important step of artificial growth.Therefore need to set up the practical technique of body odour bavin cutting propagation.
The content of the invention
It is an object of the invention to provide a kind of body odour bavin cuttage breeding method, the method can significantly increase body odour bavin
Cutting propagation survival rate, can be to realize that its large-scale planting and industrialization development are utilized to lay the foundation.
The technical solution used in the present invention：The body odour bavin annual shoot for growing under different times clip difference dark conditions first
Cuttings is placed in cuttage after immersion in the auxin of variable concentrations and the basic element of cell division by bar as cuttage material.Walk by more than
Suddenly most suitable each single factor test condition is filtered out, is then matched somebody with somebody with cytokinin concentration using orthogonal test growth regulation element and is when soaked
The bubble time, it is determined that being suitable to the treatment conditions of body odour bavin cutting propagation, and then set up body odour bavin Technique on Cuttage Propagation method.By cuttage
The body odour bavin plant for surviving is transplanted, you can obtain its nascent plant in batches.
Preferably in the bud dormancy phase（Early December is to mid-January）Under clip moderate dark conditions（Light transmittance is 20%～25%）It is raw
The cuttings pruned is immersed in the mgL of 6-BA 300 by long body odour bavin annotinous branch as cuttage material-1 + NAA
200 mg·L-1Solution in soak and carry out after 1 h cuttage, cuttings is transplanted after surviving, you can obtain nascent plant.
Comprise the following steps that：
Step 1：It is chosen at the material of the body odour bavin annotinous branch as cuttage of growth under the sunshade net that light transmittance is 20%～25%
Material；
Step 2：In the bud dormancy phase（Early December is to mid-January）The stalwartness for select the no disease and pests harm in step 1, having no mechanical damage
Branch is used as cutting.The cm of cuttings length 20～25 of clip, top contains 2 away from the cm truncations of node 1～2, base portion beveling, each cuttings
～3 sections；
Step 3：The cm of cuttings base portion 5～10 in step 2 is inserted into the mgL of 6-BA 300-1 + NAA 200 mg·L-1It is molten
1 h is soaked in liquid；
Step 4：Cuttings after soaking in step 3 is carried out into cuttage by the distance between rows and hills of 10 cm × 10 cm.By slotting machine before cuttage
Ground levelling and pour it is permeable after be covered with transparent plastic mulch film.Cuttings base portion 1/3 is inserted in slotting machine during cuttage.Pour in good time after cuttage
Water.Bud point in mid-March cuttings starts to sprout, and grows branches and leaves, and early April starts root of hair.
Step 5：The body odour bavin cuttings taken root in step 4 is transplanted to land for growing field crops by the distance between rows and hills of 0.8 m × 0.5 m.Transplant
Shi Jinliang retains rhizosphere soil, and waters in good time, keeps suitable soil moisture.
The beneficial effect that the present invention reaches：
Seed sprouts more difficult under body odour bavin natural conditions, and natural propagation rate is low.The present invention is by screening, optimization body odour bavin skewer
The relevant condition of breeding is inserted, the effective technology of its sapling multiplication is built, so as to raw material needed for the industrialization development for the resource
Scaled artificial planting established good basis.
Specific embodiment,
With reference to embodiment, the present invention is described in further detail.Such as relating to numerical value or number ratios in embodiment, such as
Mass figures or mass ratio are referred both to without indicating.
Embodiment 1：The screening of cuttage material and cutting time,
Vegetable material body odour bavin in the present invention（Premna puberulaPamp.）Pick up from Guizhou Province's Wuchuanyilaozumiaozu certainly
County is controlled, is cultivated in severe shading（Light transmittance is 8%～10%）, moderate shading（Light transmittance is 20%～25%）And three kinds of bars of natural light
Under part.
Respectively at the bud dormancy phase（Early December is to mid-January）, the budding period（Late January is to late Febuary）And leaf stretching period（On March
Ten days is to late April）The annual no disease and pests harm grown under the above-mentioned three kinds of illumination conditions of clip, the healthy and strong branch having no mechanical damage enter
Row cuttage.The cm of clip cuttings length 20～25, top is saved away from the cm truncations of node 1～2, base portion beveling, each cuttings containing 2～3,
Count its survival rate.Conclusion：The cuttings of body odour bavin cutting propagation should choose the branch of bud dormancy phase under moderate shading（Table 1）.
Embodiment 2：The screening of plant growth regulating substance,
The basically identical cutting of clip growth conditions（Cutting clip method is with embodiment 1）, by cuttings be respectively placed in concentration for 50,
100、200、400 mg·L-16-BA, KT, NAA, IBA solution in soak cuttage after 2 h, with clear water as control, count its into
Motility rate.Conclusion：In the mgL of 6-BA 200-1、6-BA 400 mg·L-1With the mgL of NAA 200-1The body odour soaked in solution
Bavin cuttings, cuttage survival rate is higher（Table 2）.
Embodiment 3：The optimization of Cutting conditions,
Body odour twigs bar under bud dormancy phase clip moderate dark conditions, is set with 6-BA, NAA and soak time of variable concentrations
Meter Three factors-levels orthogonal test, cuttage after process counts its survival rate.Conclusion：By the cuttings mgL of 6-BA 300-1 +
NAA 200 mg·L-1Solution soaks 1 h, and cuttage survival rate is higher（Table 3）.
Embodiment 4：Transplant,
The body odour bavin plant taken root in embodiment 3 is transplanted to land for growing field crops by the distance between rows and hills of 0.8 m × 0.5 m, is tried one's best during transplanting
Retain rhizosphere soil, and water in good time.
Note：Above-described embodiment 1, example 2 are single factor experiment, and the same year is carried out；Embodiment 3 is orthogonal test, and next year is carried out.
Present invention efficiently solves in body odour bavin scaled artificial planting its seedling breeding problem, be to realize its resource forever
Continuous utilization is had laid a good foundation with industrialization development.It should be noted that：For the person of ordinary skill of the art,
Under the premise without departing from the principles of the invention, some deformations and improvement can also be made, these deformations and improvement belong to this
Bright protection domain.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (3)
1. a kind of body odour bavin cuttage breeding method, it is characterised in that：The fox grown under bud dormancy phase clip moderate dark conditions
The cuttings pruned is immersed in 6-BA 300mgL by smelly bavin annotinous branch as cuttage material-1+NAA 200mg·L-1
Solution in soak 1h after carry out cuttage, cuttings is transplanted after surviving, you can obtain nascent plant.
2. body odour bavin cuttage breeding method according to claim 1, it is characterised in that the method is comprised the following steps that：
Step 1：The body odour twigs bar grown under the sunshade net that light transmittance is 20%～25% is chosen at as cuttage material；
Step 2：No disease and pests harm, the healthy and strong branch that has no mechanical damage in bud dormancy phase early December to mid-January selects step 1
Bar is used as cutting；
Step 3：Cuttings 5～10cm of base portion in step 2 is inserted into 6-BA 300mgL-1+NAA 200mg·L-1Soak in solution
Bubble 1h；
Step 4：Cuttings after soaking in step 3 is carried out into cuttage by the distance between rows and hills of 10cm × 10cm；
Step 5：The body odour bavin cuttings taken root in step 4 is transplanted to land for growing field crops by the distance between rows and hills of 0.8m × 0.5m.
3. a kind of body odour bavin cuttage breeding method according to claim 2, it is characterised in that：Prepare skewer before cuttage is carried out
Slotting machine, soil deeptillage 20cm, by wide 1m point of railway carriage or compartment, railway carriage or compartment face is flattened and pours permeable by deep 20cm, railway carriage or compartment spacing 40cm, is covered with transparent modeling
Material mulch film is with heat and moisture preserving.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887084.4A CN106613093A (en) | 2016-10-12 | 2016-10-12 | Premna puberula cutting propagation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887084.4A CN106613093A (en) | 2016-10-12 | 2016-10-12 | Premna puberula cutting propagation method |
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| CN106613093A true CN106613093A (en) | 2017-05-10 |
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ID=58855184
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610887084.4A Pending CN106613093A (en) | 2016-10-12 | 2016-10-12 | Premna puberula cutting propagation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369230A (en) * | 2020-11-16 | 2021-02-19 | 上海上房园林植物研究所有限公司 | Open-air cutting propagation method of vitex agnus-castus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
| CN103999866A (en) * | 2014-04-28 | 2014-08-27 | 深圳市新华南方生物科技股份有限公司 | Rooting induction agent preparation method |
-
2016
- 2016-10-12 CN CN201610887084.4A patent/CN106613093A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
| CN103999866A (en) * | 2014-04-28 | 2014-08-27 | 深圳市新华南方生物科技股份有限公司 | Rooting induction agent preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 徐利等: "狐臭柴扦插繁殖技术初步研究", 《山地农业生物学报》 * |
| 胡孔峰等: "《植物组织培养技术及应用》", 31 December 2006, 河南科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112369230A (en) * | 2020-11-16 | 2021-02-19 | 上海上房园林植物研究所有限公司 | Open-air cutting propagation method of vitex agnus-castus |
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Application publication date: 20170510 |