CN106520582A - Straw microbial decomposition agent and preparation method thereof - Google Patents
Straw microbial decomposition agent and preparation method thereof Download PDFInfo
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- CN106520582A CN106520582A CN201611031860.7A CN201611031860A CN106520582A CN 106520582 A CN106520582 A CN 106520582A CN 201611031860 A CN201611031860 A CN 201611031860A CN 106520582 A CN106520582 A CN 106520582A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/16—Yeasts; Culture media therefor
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to the field of a microbial technology and relates to a straw microbial decomposition agent composed of a plurality of microbial probiotics and a preparation method thereof. The straw microbial decomposition agent has a low cost and a small amount of use, is convenient to use and can accelerate the decay of the crop waste straws. Straws are decomposed to form simple organic matters and humus so that soil is loosed and soil pH is improved. The composite straw microbial decomposition agent forms factors based on soil humic acid and humus and decomposes straw cellulose. The straw microbial decomposition agent comprises, by weight, 15 to 32 portions of yeast powder, 15 to 21 portions of filamentous fungus powder and 43 to 67 portions of cellulose-decomposing bacterium powder. The straw microbial decomposition agent has effects of fertilizing soil, enhancing the ability of preserving moisture and fertility and improving the comprehensive productivity of soil, has the advantages of small dosage, use convenience and wide application range, and prevents the environmental pollution caused by crop wastes.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of straw being made up of multiple-microorganism probiotics is micro-
Biological decomposing agent and preparation method thereof.
Background technology
The open Agro-ecological System of the long-term high load capacity being characterized with high multiple crop index so that the unique resource of agricultural
Arable land can't bear the heavy load, and bring larger negative effect to agricultural.It is big that the high agricultural planting area of one side multiple crop index produces
Amount straw rubbish has become the polluter of contaminated land, water source, air；The raising of another aspect multiple crop index causes chemical fertilizer
Consume substantial increase, the physicochemical character of soil is drastically deteriorated, adjust the soil water, fertilizer, air and heat agron year by year under
Drop, Soil Microorganism quantity decline, and the content of organic matter is fewer and feweri, soil compaction acidifying, and the composite fertilizer power in arable land is year by year
Decline.And soil compaction, organic nutrient are reduced, soil fertility declines so that crop yield becomes extremely difficult.
It is bright and clear that the appearance of biological organic fertilizer causes the solution of this problem to be able to, and it is organic that biological organic fertilizer can speed up straw
The decomposition rate of thing, can effectively adjust Ecological Distribution of Soil Microorganisms system again towards the energy and regeneration power circulation profit for being conducive to ploughing
Developed with aspect, on the premise of crop yield is guaranteed, the deterioration of soil environment can be made to obtain control even improves.At present
There are various biological organic fertilizers on market, but still suffer from shortcoming to a great extent, mainly have the following aspects：Product quality
It is unstable, 2, need to add a certain amount of other fertilizer to strengthen fertility, 3, product usage amount it is big, complex operation.Such as Japan
B.Y.M-Food, be one kind with enzymatic microorganism Inoculant directly as Organic substance compost Inoculant, its main body is：Bacillus
(Bacillus sp) and Flavobacterium (Flavobacterium sp), Lactobacilluss (Lactobacillus sp), yeast
Category (Saccharomyces sp), pichia (Pichia sp), Rhodotorula (Rhodotorula sp), in mycete
Rhizopus (Rhizopus sp).The product usage amount is big, need to be complicated with inoculation bacterium 2kg, operational approach per 500kg, will be organic
Material collection compost, timing turning, and need to add a certain amount of organic and chemical fertilizer to strengthen fertility.Domestic biology has
The rotten bar spirit microbial inoculum of machine fertilizer product such as Foshan, these products are mainly anaerobic fermentation microorganism community structure, and mu consumption is equal
In more than 2kg, in addition it is also necessary to add some measures for enriching fertilizer.And its inoculation is expansion bacterium, if passing through due to expanding bacterium
The breeding in dry generation, the less stable of hereditary character are easy to cause product effect unstable.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of low cost, and usage amount is little, easy to use, can accelerate crops
Waste straw decomposition.Straw decomposition forms simple Organic substance and humus, chesson, improved soil acid, base number it is compound
Stalk microbe decomposing agent.
Composite stalk microorganism-decomposing agent of the present invention is that humus form the factor, in decomposing straw according to soil humic acid
Cellulose, does cellulose and is designed, including the component of following weight proportion：Yeast mycopowder 15-32 parts, filamentous fungis mycopowder
15-21 parts, cellulose-decomposing bacteria mycopowder 43-67 parts.
Yeast of the present invention is Pichia sp. (Pichia fermentans).
Filamentous fungis of the present invention are black green trichoderma (Trichoderma atroviride).
Cellulose-decomposing bacteria of the present invention includes Bacillus pumilus (Bacillus pumilus), highland bacillus
(Bacillus alitudinis), bacillus subtilises (Bacillus subtilis), bacillus (Bacillus
Cohn), Brevibacillus laterosporus (Other Strain Name), staphylococcus saprophyticus (Staphylococcus
Saprophyticus), in Acinetobacter baumannii (Acinetobacter baumannii) any one or its mixing.
In order to adjust pH value of all kinds of microbial bacterias during the growth metabolism of arable land, it is to form simple humus to create bar
Part, composite stalk microorganism-decomposing agent of the present invention also include Lactococcus, and Lactococcus of the present invention are pentose piece
Coccus.
The composite stalk microorganism-decomposing agent of Lactococcus is with the addition of, the weight proportion of component is as follows：Yeast mycopowder 15-
32 parts, filamentous fungis mycopowder 15-21 parts, cellulose-decomposing bacteria mycopowder 43-67 parts, Lactococcus mycopowder 4-9 parts.
Wherein, the cellulose-decomposing bacteria of composite stalk microorganism-decomposing agent of the present invention adopts following weight proportion strain combination：
Composite stalk microorganism-decomposing agent of the present invention can be obtained by the purchase of existing commercial channel using raw material, Huo Zhetong
Cross separating-purifying to obtain.Such as：Pediococcus pentosaceuss buying is from southwestern microorganism fungus kind station；Black green trichoderma, Pichia sp., short and small spore
Bacillus, highland bacillus, bacillus subtilises, bacillus, Brevibacillus laterosporus, staphylococcus saprophyticus, Boydii are motionless
Bacillus bacterium is all to be isolated from forest and crop land soil.
The separation of above-mentioned separate microorganism with culture medium is：Using conventional medium, such as potato sucrose culture medium,
Rose bengal medium, nutrient agar, meat soup fine jade born of the same parents' culture medium, Czapek ' s agar culture mediums.Separating technology is：Take
Soil sample, Ensure Liquid liquid breeding, filters, takes clear liquid and be inoculated into culture medium, the representational single bacterium colony of picking, microscopy and coating
Separate it is repeated multiple times, until isolate pure culture be DHA identification till.
The preparation method of composite stalk microorganism-decomposing agent of the present invention, comprises the steps：Planted using conventional method
Son culture, industrial amplification culture, culture fluid concentration, mycopowder is made in lyophilization, then by aforementioned composite stalk microorganism-decomposing
The component of agent weighs mycopowder mixing and obtains final product.
The using method of composite stalk microorganism-decomposing agent of the present invention is, by composite stalk microorganism-decomposing agent of the present invention with
Water mixes, and is sprayed on crop waste straw.Composite stalk microorganism-decomposing agent of the present invention has operational approach, need not
Chemistry fertilizer is added separately, without the operation for separately carrying out compost and turning.
Composite stalk microorganism-decomposing agent of the present invention and preparation method thereof is had the beneficial effect that with using method：
Composite stalk microorganism-decomposing agent of the present invention is to form the factor and stalk fibre according to soil humic acid, humic acid matter
Decompose and then using the different biological strain optimum organizations such as microbial starter bacterium, filamentous fungis, cellulose-decomposing bacteria, Lactococcus
Into composite stalk microorganism-decomposing agent.Composite stalk microorganism-decomposing agent of the present invention have stronger resistance, adaptability and
Decompose or degradation capability；Straw fast decoupled, cellulose can be made to be decomposed and be fermented into various organic acid, alcohols, carbon dioxide
And hydrogen, it is ensured that on the premise of existing cropping system is not destroyed, by the waste straw of crops also field.Composite stalk of the present invention
Microorganism-decomposing agent has fertilizing soil ability, strengthens retain water and nutrients ability, lifts soil Comprehensive Productivity, and using dosage
Little, easy to use, applied widely advantage, the environmental pollution that agricultural waste material can be avoided to cause.
Specific embodiment
In the embodiment of the present invention：Pediococcus pentosaceuss buying is from southwestern microorganism fungus kind station；It is black green trichoderma, Pichia sp., short
Bacillus pumiluss, highland bacillus, bacillus subtilises, bacillus, Brevibacillus laterosporus, staphylococcus saprophyticus, Bao
Family name's acinetobacter calcoaceticus bacterium is all to be isolated from forest and crop land soil.
It is above-mentioned microorganism is separated from soil the separation culture medium that adopts for：Using conventional medium, such as horse
Bell potato sucrose medium, rose bengal medium, nutrient agar, meat soup fine jade born of the same parents' culture medium, Czapek ' s agar cultures
Base.Separating technology is：Soil sampling, Ensure Liquid liquid breeding, filters, takes clear liquid and be inoculated into culture medium, and picking is representational single
Bacterium colony, microscopy and coating separate it is repeated multiple times, until isolate pure culture be DHA identification till.
The step of preparation method of composite stalk microorganism-decomposing agent of the present invention, includes：Seed training is carried out using conventional method
Support, industrial amplification culture, culture fluid concentration, mycopowder is made in lyophilization, then by aforementioned composite stalk microorganism-decomposing agent
Component weighs mycopowder mixing and obtains final product.Mycopowder preparation method wherein in present example is as follows：
Seed culture：Each microorganism fungus kind is seeded in triangular flask culture medium respectively and is cultivated, cultivation temperature is 25-37
DEG C, agitator rotating speed 130-230r/pm, incubation time 12-36 hours.
Industrial amplification culture：(1) triangular flask seed is accessed into first order seed fermentor cultivation, cultivation temperature 25-37 DEG C, machine
Tool speed of agitator 120-230r/pm, selects to be adapted to the ventilation of growth of microorganism, incubation time 12-36 hours.(2) will culture
First order seed accesses secondary seed fermentor cultivation, and cultivation temperature 25-37 DEG C, mechanical agitation rotating speed 120-230r/pm select suitable
Close the ventilation of growth of microorganism, incubation time 12-36 hours.(3) culture secondary seed is accessed into fermentor cultivation, culture temperature
25-37 DEG C of degree, mechanical agitation rotating speed 120-230r/pm select to be adapted to the ventilation of growth of microorganism, and incubation time 18-48 is little
When.
Culture fluid is concentrated：The fermentation liquid that fermentation is completed is centrifuged into supernatant, is concentrated.
Lyophilization：Concentrated solution is carried out into lyophilization, mycopowder is obtained.
The mycopowder of each microorganism is mixed in proportion and obtains final product composite stalk microorganism-decomposing agent of the present invention.
It is below example of the composite stalk microorganism-decomposing agent of the present invention in Field information.
After crop harvesting, straw is laid in into field ground, irregularly can be tiled, but uniform laying as far as possible.This
The using dosage of invention composite stalk microorganism-decomposing agent is：The straw of every mu of soil generation is using the micro- life of composite stalk of the present invention
Thing decomposing agent 5-10g, adds 1-5kg water immersion 12-48 hours, adds 50-150kg clear water, by above-mentioned composite stalk micro- life
Thing decomposing agent uniform suspension is sprayed on straw.It is preferred that the straw of every mu of soil generation is micro- using composite stalk of the present invention
Biological decomposing agent 5g, adds 5kg water to soak 24 hours, adds 100kg clear water, above-mentioned composite stalk microorganism-decomposing agent is hanged
Supernatant liquid is uniformly sprayed on straw.
1st, application example 1：The impact that composite stalk microorganism-decomposing agent of the present invention is cultivated to Caulis et Folium Lactucae sativae
The composite stalk microorganism-decomposing agent of the present invention that this application example is adopted includes the mycopowder of following weight proportion：Yeast
32 parts of bacterium mycopowder, 43 parts of cellulose-decomposing bacteria mycopowder, 9 parts of Lactococcus mycopowder, filamentous fungis mycopowder 15-21 parts.
Test method：Plot experiment arranges 3 process, 4 repetitions, and the arrangement of completely random district's groups, 5.0 meters of zone leader are wide
4.0 meters, sowing specification is 33*27cm.Protection row is stayed around experimental plot, other cultivation management measures are identical with large area production
And control consistent.
Plot experiment process is as follows：
1) reagent takes the invention described above composite stalk microorganism-decomposing agent 5g, adds 5kg water to soak 24 hours, adds
100kg clear water is sprayed at above-mentioned composite stalk microorganism-decomposing agent uniform suspension on straw.
Reagent 2) rotten bar spirit, every mu of consumption 2kg, by specification convert after appropriate soil is mixed charity on rice straw, then
Sprinkling 100kg water/mu.
Reagent 3) clear water matched group：By 100kg water/mu sprinkling.
Division of cells on request after site preparation, per mu evenly laid out down on the farm with 600kg straws, selects ad eundem Caulis et Folium Lactucae sativae Seedling to move
Plant, per cell 200 plants of Caulis et Folium Lactucae sativaes, then imposing composite stalk microorganism-decomposing agent of the present invention, rotten bar spirit, clear water respectively carries out corruption
Solution, per mu imposes 20.2kg carbamide, and after 2 months, point cell list is received and calculates yield.As a result it is as follows：
1 Caulis et Folium Lactucae sativae of table applies the clump count respectively become thoroughly decomposed after condition on rice straw
| Process group | 10th day | 20th day | 30th day |
| Decomposing agent of the present invention | 4.90 | 10.20 | 12.80 |
| Rotten bar spirit | 4.2 | 9.60 | 12.2 |
| Water | 0.70 | 0.60 | 0.60 |
Experimental result shows：After rice straw inoculation straw composite stalk microorganism-decomposing agent of the present invention, breeding colony is rapid,
Rotten bar spirit of the clump count apparently higher than clear water matched group and reference.
Table 2 respectively becomes thoroughly decomposed decomposition of the condition to rice straw
| Process group | 30th day | 38th day | 46th day |
| Decomposing agent of the present invention | Preliminary decomposition | Moderate decomposition | Complete decomposition |
| Rotten bar spirit | Non- decomposition | Preliminary decomposition | Complete decomposition |
| Water | Non- decomposition | Preliminarily softened | Soften |
Experimental result shows：Composite stalk microorganism-decomposing agent of the present invention is fast to the decomposition of the covering rice straw of Caulis et Folium Lactucae sativae
Speed, effect are substantially better than the rotten bar spirit of clear water matched group and reference.
2nd, application example 2：Impact of the composite stalk microorganism-decomposing agent of the present invention to radish cultivation
The composite stalk microorganism-decomposing agent of the present invention that this application example is adopted includes the mycopowder of following weight proportion：
15 parts of yeast mycopowder, 54 parts of cellulose-decomposing bacteria mycopowder, 9 parts of Lactococcus mycopowder, Bacillus pumilus mycopowder 10
Part, highland bacillus mycopowder 14-21 parts, 25 parts of bacillus subtilises mycopowder, 21 parts of bacillus mycopowder.
1) reagent takes the invention described above composite stalk microorganism-decomposing agent 5g, adds 5kg water to soak 24 hours, adds
100kg clear water, above-mentioned composite stalk microorganism-decomposing agent uniform suspension is sprayed on straw.
Reagent 2) rotten bar spirit, every mu of consumption 2kg, by specification convert after appropriate soil is mixed charity on rice straw, then
Sprinkling 100kg water/mu.
Reagent 3) clear water matched group：By 100kg water/mu sprinkling.
Test method：Division of cells on request after site preparation, presses line-spacing 30cm in cell, moves back nest 30cm and digs shallow den and broadcasts trailing plants
Foretell and plant.Per mu evenly laid out down on the farm with 500kg straws, then imposes composite stalk microorganism-decomposing agent of the present invention, rotten bar respectively
Spirit, clear water carry out decomposition, carry out equal Seedling final singling, and per mu imposes 8.0kg carbamide after Radish Emerging, and after 2 months, point cell list is received and calculated
Yield.As a result it is as follows：
Table 3 applies impact of the condition to radish yield of respectively becoming thoroughly decomposed
| Process group | Average yield per mu (kg) | Mu volume increase (kg is contrasted with clear water group) | Rate of growth (is contrasted with clear water group) |
| Decomposing agent of the present invention | 2171.1 | 110 | 5.345% |
| Rotten bar spirit | 2132.8 | 71.7 | 3.48% |
| Water | 2061.1 |
Experimental result shows：Radix Raphani increases than the per mu yield of clear water matched group using after composite stalk microorganism-decomposing agent of the present invention
110.0kg, has increased production and has checked for 5.345%, Jing PLSD, and significantly, i.e., composite stalk microorganism of the present invention is rotten for both volume variances
The ripe dose of rotten bar spirit for being substantially better than reference process after Radix Raphani per mu yield incrementss and increment rate.
3rd, application example 3：Impact of the composite stalk microorganism-decomposing agent of the present invention to Caulis Sacchari sinensis straw decomposing
The composite stalk microorganism-decomposing agent of the present invention that this application example is adopted includes the mycopowder of following weight proportion
15 parts of yeast mycopowder, 54 parts of cellulose-decomposing bacteria mycopowder, 9 parts of Lactococcus mycopowder, Bacillus pumilus mycopowder 10
Part, highland bacillus mycopowder 14-21 parts, 25 parts of bacillus subtilises mycopowder, 21 parts of bacillus mycopowder, filamentous fungis bacterium
Powder 21.
1) reagent takes the invention described above composite stalk microorganism-decomposing agent 5g, adds 5kg water to soak 24 hours, adds water dilute
Release standby to 100kg.
The water preparation 1000g of reagent 2) " Jin Kuizi " microorganism decomposition agent is standby.
Reagent 3) clear water matched group：Consumption ensures Caulis Sacchari sinensis straw of drenching.
Test method：1070kg Caulis Sacchari sinensis straws are equally divided into into three heaps, 365kg are nearly weighed per heap, after Caulis Sacchari sinensis straw pile
Drenched with clear water, add a cover appropriate fine earth, impose composite stalk microorganism-decomposing agent of the present invention, rotten bar spirit, clear water control respectively, so
Plastic covering thin film, carries out decomposition afterwards, observes a clump count, observe a clump count after complete decomposition again after 7 days.After 2 weeks
Turning is carried out to three treatment groups, straw heap surrounding is turned to into centre without the straw for becoming thoroughly decomposed and is allowed which fully to become thoroughly decomposed.
Wherein, will be drenched with clear water after Caulis Sacchari sinensis straw pile, add a cover appropriate fine earth, impose composite stalk of the present invention respectively micro-
The concrete grammar that biological decomposing agent, the spirit of rotten bar, clear water control carry out decomposition is as follows：1) by Caulis Sacchari sinensis straw pile to 30cm Gao Houyong
Clear water is drenched, and adds a cover appropriate fine earth, respectively by 1/3 composite stalk microorganism-decomposing agent diluent, 1/3 gold medal certain herbaceous plants with big flowers " microorganism urges
Rotten agent water preparation, addition 1kg carbamide, 1kg potassium dihydrogen phosphates, are sprayed at after stirring on Caulis Sacchari sinensis straw；And clear water group is then direct
Pile will be sprayed at and added a cover on the Caulis Sacchari sinensis straw of fine earth.2) Caulis Sacchari sinensis straw pile 30cm high again is drenched with clear water, plus
Cover appropriate fine earth, respectively by 1/3 composite stalk microorganism-decomposing agent diluent, 1/3 gold medal certain herbaceous plants with big flowers " microorganism decomposition agent water preparation, plus
Enter 1kg carbamide, 1kg potassium dihydrogen phosphates, be sprayed at after stirring on Caulis Sacchari sinensis straw；And clear water group then will directly be sprayed at pile
And add a cover on the Caulis Sacchari sinensis straw of fine earth.3) according to step 2) operation by the total height pile of Caulis Sacchari sinensis straw to 100cm, point
Not by remaining 1/3 composite stalk microorganism-decomposing agent diluent, 1/3 gold medal certain herbaceous plants with big flowers " microorganism decomposition agent water preparation, add 1kg urine
Element, 1kg potassium dihydrogen phosphates, are sprayed at after stirring on Caulis Sacchari sinensis straw；And clear water group then will directly be sprayed at pile and add a cover
On the Caulis Sacchari sinensis straw of fine earth.
Become thoroughly decomposed result：
1st, 7 days after testing, decomposing agent group of the present invention, golden certain herbaceous plants with big flowers subgroup, clear water group respectively extract 10 Caulis Sacchari sinensis straw observations：
1) decomposing agent group of the present invention：Straw color become black gray expandable, it is softer, length be 30-80cm, width be 1.5-3cm, portion
Divide straw broken with hand rubbing, have at bacterium colony group 15 on 10 Caulis Sacchari sinensis straws.
2) golden certain herbaceous plants with big flowers subgroup：Straw color become black gray expandable, it is softer, length be 50-100cm, width be 1.5-3cm, part straw
Stalk can be broken with hand rubbing, has at bacterium colony group 6 on 10 Caulis Sacchari sinensis straws.
3) clear water group：Straw color is withered yellow, and length is 80-150cm, width is 1.5-3cm, can not use hand rubbing
It is broken, straw also no bacterium colony group.
2nd, test to the 30th day after becoming thoroughly decomposed completely, decomposing agent group of the present invention, golden certain herbaceous plants with big flowers subgroup, clear water group respectively extract 10 Caulis Sacchari sinensis
Straw is observed：
1) decomposing agent group of the present invention：Straw color is black, and length is 5-30cm, width is 1.5-3cm, can be used substantially
Hand rubbing is broken, and every straw has more saprophytic bacteria.
2) golden certain herbaceous plants with big flowers subgroup：Straw color is black, and it is 1.5-3cm that length is 10-30cm, width, and part straw can use handss
Rub broken with the hands, on the straw of part, have saprophytic bacteria.
3) clear water group：Straw color is withered yellow, and length is 80-150cm, width is 1.5-3cm, can not use hand rubbing
It is broken, straw also substantially not no saprophytic bacteria.
Conclusion：The golden certain herbaceous plants with big flowers subgroup of composite stalk microorganism-decomposing agent of the present invention and clear water matched group have
Really.
Applicant is also directed to wheat stalk, rape stalk, discarded fresh dish leaf (such as Chinese cabbage, green vegetable, Caulis et Folium Lactucae sativae) to be carried out also
Field is tested, and effect of not only becoming thoroughly decomposed is good, and for the crop for going back field, such as Oryza sativa L., Rhizoma Solani tuber osi, the growth of Semen Tritici aestivi has to supplement well and support
Material, promotes the effect of growth.
Composite stalk microorganism-decomposing agent of the present invention takes composite stalk microorganism-decomposing of the present invention through toxicity detection simultaneously
Agent 5g, plus deionized water 20ml, mixing are obtained final product.Respectively to 10 male mices and 10 female mices by " once to greatest extent
Test " requires to be tested, and dose design is 5g/kgBW for male, and female is 5g/kgBW, by the oral gavages of 0.2ml/10gBW
Give tested material, animal fasting overnight before gavage, free water give normal diet after gavage, observe 14 days, record sign and
, there is experimental animal and grow normally, have no poisoning sign and death, gross anatomy no abnormality seen in death condition.Conclusion：It is tested
Thing is more than 5g/kgBW for male to the oral median lethal dose(LD 50) of mice dosage form, and female is more than 5g/kgBW；Given birth to according to microbial manure
In thing safety general technical criteria (NY-1109-2006), acute toxicity grading criteria judges, composite stalk microorganism of the present invention is rotten
Ripe dose is actual nontoxic.
Arsenic and compound (in terms of As) are less than or equal to less than or equal to 75mg/kg, cadmium and compound (in terms of Cd) after testing
Less than or equal to 100mg/kg, chromium and compound (in terms of Cr) are less than or equal to 150mg/ for 10mg/kg, lead and compound (in terms of Pb)
Kg, hydrargyrum and compound (in terms of Hg) are as a result shown and meet GB18877-2002 less than or equal to 5mg/kg《Organic-inorganic compound mixed fertilizer
Material》Standard test is required.
To sum up, composite stalk microorganism-decomposing agent consumption of the present invention is little, and strain growth is fast, can soften rapidly decomposition straw,
Increase income crop yield, a kind of brand-new selection is provided to process agricultural waste material straw.
Claims (6)
1. composite stalk microorganism-decomposing agent, it is characterised in that：Including the component of following weight proportion：Yeast mycopowder 15-32
Part, filamentous fungis mycopowder 15-21 parts, cellulose-decomposing bacteria mycopowder 43-67 parts.
2. composite stalk microorganism-decomposing agent according to claim 1, it is characterised in that：Also include Lactococcus mycopowder,
The weight proportion of component is as follows：Yeast mycopowder 15-32 parts, filamentous fungis mycopowder 15-21 parts, cellulose-decomposing bacteria mycopowder 43-67
Part, Lactococcus mycopowder 4-9 parts.
3. composite stalk microorganism-decomposing agent according to claim 1 and 2, it is characterised in that：At least meet following arbitrary
：
Described yeast is Pichia sp.；
Described filamentous fungis are black green trichoderma；
Described cellulose-decomposing bacteria includes Bacillus pumilus, highland bacillus, bacillus subtilises, bacillus, side
Spore bacillus cereuss, staphylococcus saprophyticus, any one in Acinetobacter baumannii or its mixing.
4. composite stalk microorganism-decomposing agent according to claim 3, it is characterised in that：Described cellulose-decomposing bacteria is adopted
Following weight proportion strain combination：
5. composite stalk microorganism-decomposing agent according to claim 3, it is characterised in that：Described cellulose-decomposing bacteria is adopted
Following weight proportion strain combination：
6. the preparation method of composite stalk microorganism-decomposing agent described in any one of claim 1-5, it is characterised in that：Including as follows
Step：Seed culture is carried out using conventional method, industrial amplification culture, culture fluid concentration, lyophilization are made mycopowder, then pressed
The component of aforementioned composite stalk microorganism-decomposing agent weighs mycopowder mixing and obtains final product.
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