CN106416725A - Planting method for pseudo-ginseng - Google Patents
Planting method for pseudo-ginseng Download PDFInfo
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- CN106416725A CN106416725A CN201610928760.8A CN201610928760A CN106416725A CN 106416725 A CN106416725 A CN 106416725A CN 201610928760 A CN201610928760 A CN 201610928760A CN 106416725 A CN106416725 A CN 106416725A
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- radix notoginseng
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- notoginseng
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B77/00—Machines for lifting and treating soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention belongs to the field of traditional Chinese medicine planting and particularly relates to a planting method for pseudo-ginseng. The method comprises the following steps: (S1) selecting planting land; (S2) preparing the land; (S3) selecting seeds; (S4) cultivating the pseudo-ginseng seedlings; (S5) transplanting; (S6) managing growth; and (S7) harvesting. According to the method provided by the invention, the problem of successive cropping obstacle of pseudo-ginseng is solved and the pseudo-ginseng yield and quality are increased.
Description
Technical field
The invention belongs to Chinese medicine plant field, and in particular to a kind of implantation methods of Radix Notoginseng.
Background technology
Radix Notoginseng (Panaxnotoginseng (buke.) F.H.Chen) be araliaceae ginseng plant, with blood circulation promoting and blood stasis dispelling,
Blood circulation promoting and blood stasis dispelling, the effect of reducing swelling and alleviating pain and treatment cardio-cerebrovascular diseases, is FUFANG DANSHEN DIWAN, YUNNAN BAIYAO, XUESAITONG, piece
The important source material of the Chinese patent medicines such as young Huang.Radix Notoginseng is tame medicinal plants, and cultivation history is nearly 400 years, and main product is in Yunnan, wide
West, and Yunnan mountain of papers is its Genuine producing area.
Radix Notoginseng is the cloudy C3 plant of typical happiness, belongs to the perennial root plant that no-tillage the moon covers cultivation, and on ground, the time is long, and
Its property happiness is warm dark and damp.But, the continuous cropping obstacle of Radix Notoginseng seriously constrains the development of Radix Notoginseng industry.Notoginseng Effects master
Show as：Percentage of seedgermination, germination index substantially reduce；Planting percent and plant height are remarkably decreased；Disease is serious, and yield declines.
Radix Notoginseng plantation one batch after continue continuous cropping be easily caused the generation of the diseases such as root rot with popular；Crop rotation interval year limit for length, growing area
Extension, medical material genuineness is difficult to ensure that.
Notoginseng obstacle is primarily due to notoginseng root rot and Radix Notoginseng Auto toxicity.The pathogen monoid of notoginseng root rot
Mainly include partly to know that subphylum fungi is damaged post spore bacterium (Cylindrocarpondestructans (zinss.) Scholton), intended
Ginseng post spore bacterium (Cindroncarpon TYLOFS-panacl ' s (Hild)), sturdy post spore bacterium
(ClindroncarponrobustaHild.), Radix Ginseng post spore bacterium (CylindrocarponPanacisMatuoetMiyaza-
Wa), Fusarium oxysporum (FusariumoxysporumSchlecht), Fusarium solani (Fusariumsolani (Mart.)
Sacc.), Rhizoctonia solani Kuhn (Rhizoctoniasolani), Verticillium sp bacterium (Verticillium sp.) and Phoma
(Phomasp) and deformation door antibacterial Pseudomonas alba (Pseudomonsa sp.).
Shen Qing Publication day is on April 13rd, 2016, and application publication number is the Chinese invention patent application of CN105471951A,
A kind of notoginseng planting method of high yield is disclosed, the method is in the Bactericidal fertilizer substrate of field furrow surface paving 1-2cm thickness；Sterilization fertilizer
Material substrate includes the decomposed manure of 80-90% and the sterilization group of 10-20%, and sterilization group is by Radix Sophorae Flavescentiss, 40-80ml/g kasugarnycin
It is mixed, its weight ratio is 1:1；Sow after soaking seed in salicylic acid of the concentration for 150-250mg/L in sterilization substrate, then
Nature Germination And Seedling is bred after covering soil.Employ Bactericidal fertilizer substrate in the method to process field furrow, sterilization
Sterilization group in fertilizer is mixed by Radix Sophorae Flavescentiss and kasugarnycin, and its weight ratio is 1:1；But, the method can not be good
Solve the problems, such as notoginseng obstacle.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of implantation methods of Radix Notoginseng, to solve notoginseng obstacle, carries
The yield and quality of high Radix Notoginseng.
In order to solve above-mentioned technical problem, the technical scheme is that：
A kind of implantation methods of Radix Notoginseng, comprise the steps：
S1, selection planting site
Select that the soil is porous is fertile, well-drained neutral or slant acidity sand loam forest land, and the light transmittance in the forest land
For 20～30%；Radix Notoginseng is the cloudy C3 plant of typical happiness, has harsh requirement to intensity of illumination.
S2, site preparation
In the Radix Notoginseng transplantation of seedlings previous moon, per mu apply 200～250kg of quick lime, 300～400kg of Herba Artemisiae Annuae, Radix Artemisia ordosicae 100～
150kg, 10～20kg of Bulbus Allii, 50～80kg of Radix Et Rhizoma Rhei, 50～70kg of Radix Sophorae Flavescentiss, 40～70kg of Herba polygoni hydropiperis and 10～30kg of Pericarpium Zanthoxyli, deep ploughing
Dig；5 days before Radix Notoginseng transplantation of seedlings, per mu is applied 3000～5000kg of farm manure, and intertillage is digged；
The inventors found that Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentiss, Herba polygoni hydropiperis and the Pericarpium Zanthoxyli of said ratio can be very
Good suppression or killing cause the flora of root rot.
S3, seed are selected
The full mellow fruit tied by 3 years above Radix Notoginseng plant of life is chosen, is rubbed with the hands and peel is removed, clean, obtain Radix Notoginseng kind
Son；
S4, Radix Notoginseng nursery
1) culture medium prepares：Callus inducing medium：B5+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L KT, adjusts pH 5.6～6.0；Calluss division culture medium：MS+1.5mg/L 2,4-D+
0.1mg/L IBA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamycin, adjusts pH 5.6～6.5；Take root
Inducing culture：MS+1.0mg/L NAA+1.0mg/L IBA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3, adjusts pH
5.6～6.5；
2) explant prepares：Take the Panax notoginseng seeds that chooses in step S3 and explant is done, by Panax notoginseng seeds in 75% ethanol
60s is soaked, then in 0.1%HgCl220～30min is soaked in solution, with aseptic water washing 5～8 times；Then, Radix Notoginseng is peelled off
The kind skin of seed；
3) it is inoculated with：The Panax notoginseng seeds for peelling off kind of skin are inoculated on callus culture, dark under conditions of 10～20 DEG C
Culture 3～5 days, then cultivates 30～40 days under conditions of 10～20 DEG C, the daily illumination of 1600～1900Lx 12 hours, obtains more
Injured tissue；
4) successive transfer culture:Calluss are transferred on calluss division culture medium, 10～20 DEG C, 1800～
Under conditions of the daily illumination of 2000Lx 8～12 hours 15～20 days subcultures once, proliferation times be:8～10, obtain Regenerated plant；
5) root culture：Regenerated plant is transferred in root induction culture medium, 15～20 DEG C, 1800～2000Lx daily
Illumination was cultivated 10～15 days under conditions of 8～12 hours, seedling of must taking root；
6) seedling exercising：Seedling of taking root is taken out from culture medium, clean root culture medium, plants the sand soil seedbed in greenhouse
In, natural lighting, seedling exercising 5～8 days, obtain Radix Notoginseng seedling；
S5, transplanting
Radix Notoginseng seedling root is dipped after trichoderma harzianum bacterium solution, in the forest land that puts in order in transplanting to S2 step, spacing in the rows
30cm, line-spacing 40cm, per mu is applied 10～15kg of azophoska, and the constituent weight ratio of the azophoska is, carbamide：Cross phosphorus
Sour calcium:Potassium sulfate=2:1:3, root water is poured, and with the ClO 2 solution that volume ratio is 1%, earth's surface is carried out spraying disappearing
Poison；
Trichoderma harzianum encloses and can mushroom out in the rhizosphere of plant, leaf, seizes the site on plant surface, forms one
Protective cover, is prevented pathogenic fungi from touching root system of plant and blade surface, is protected plant root, leaf portion with this from pathogen
Infect.
S6, growth management
First Year May applies calcium magnesium phosphate and potash fertilizer, and fertilising used trichoderma harzianum bacterium solution pouring root after one week；
Second Year and the 3rd year beginning of spring, trichoderma harzianum bacterium solution are sprayed to notoginseng planting, and per mu is applied farm manure 2000
～3000kg, the weight ratio of the farm manure constituent is, sheep stool:Cattle manure:Pig manure:Chicken manure=2:2:5:1；Apply in 4～June
Potash fertilizer, in May with trichoderma harzianum bacterium solution pouring root；
In Second Year June, flower of Radix Notoginseng a kind of sedge is beaten and remove；November to notoginseng planting per mu spread fertilizer over the fields plant ash 1000～
1500kg；
7～August plucks flower of Radix Notoginseng within 3rd year, then imposes fertiliser containing magnesium；Flower a kind of sedge is beaten except or flower of Radix Notoginseng is plucked, eliminate reproduction
Growth, can so promote nutrient growth.
Find that weeds remove in time in whole growth course；Discovery has disease strain to pull out in time；
S7, harvesting
In late October, the 3rd to early November, Radix Notoginseng is excavated when turning withered and yellow by Radix Notoginseng cane, removes stem and leaf and soil, shines
Dry preservation.
In the implantation methods of the Radix Notoginseng that the present invention is provided, it is preferable that the Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentiss, peppery
Knotweed and Pericarpium Zanthoxyli apply forest land after crushed.
In the implantation methods of the Radix Notoginseng that the present invention is provided, it is preferable that the Radix Et Rhizoma Rhei is sorrel, Rheum officinale or Tang
Ancient especially big yellow Herb.
In the implantation methods of the Radix Notoginseng that the present invention is provided, it is preferable that the fertiliser containing magnesium of applying described in step S6 is foliage-spray
The Adlerika of 10g/L, sprays to blade face dropping liquid.Fertiliser containing magnesium described in step S6 can also be the water such as magnesium chloride, magnesium nitrate
Dissolubility fertiliser containing magnesium.
In the implantation methods of the Radix Notoginseng that the present invention is provided, it is preferable that the trichoderma harzianum bacterium solution described in step S5
Concentration is 150～200g/L.
In the implantation methods of the Radix Notoginseng that the present invention is provided, it is preferable that in step S6, the described Kazakhstan thatch for pouring root is wooden
The concentration of mycete bacterium solution is 50g/L, and per plant of Radix Notoginseng irrigates 200mL；The concentration of the trichoderma harzianum bacterium solution for spraying is
30g/L, per mu sprays 100L.
The present invention provide Radix Notoginseng implantation methods in, it is preferable that the forest land for plantation Fructus oleae europaeae, Semen Juglandiss, Folium Eriobotryae,
Fructus Vitis viniferae or the field of Fructus Citri grandiss.
Using technique scheme, due to the method nursery using tissue culture, the gained Radix Notoginseng Seedling uniformity, Radix Notoginseng kind has been saved
Sub- consumption；The Radix Notoginseng transplantation of seedlings previous moon with Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentiss, Herba polygoni hydropiperis and Pericarpium Zanthoxyli to forest land at
Reason, suppresses or has killed the pathogenic bacteria for causing root rot in timbered soil；Root dipping, filling are carried out using trichoderma harzianum bacterium solution
Root and spray, pathogen infection Radix Notoginseng root system is prevented, Radix Notoginseng survives strain rate and more than 94% is reached, solve notoginseng barrier
Hinder.
Specific embodiment
Below the specific embodiment of the present invention is described further.Here is it should be noted that for these enforcements
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.Additionally, invention described below
As long as involved technical characteristic does not constitute conflict each other and can just be mutually combined in each embodiment.
Embodiment 1
A kind of implantation methods of Radix Notoginseng, comprise the steps：
S1, selection planting site
Select that the soil is porous is fertile, the forest land of the plantation Fructus oleae europaeae of well-drained neutral or slant acidity sand loam, and
The light transmittance in the forest land is 20%；
S2, site preparation
In the Radix Notoginseng transplantation of seedlings previous moon, per mu apply quick lime 200kg, Herba Artemisiae Annuae 400kg, Radix Artemisia ordosicae 120kg, Bulbus Allii 10kg,
Sorrel 80kg, Radix Sophorae Flavescentiss 50kg, Herba polygoni hydropiperis 40kg and Pericarpium Zanthoxyli 30kg, quick lime, Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, sorrel, Radix Sophorae Flavescentiss,
Herba polygoni hydropiperis and Pericarpium Zanthoxyli are to apply forest land after crushed, and Herba Artemisiae Annuae, Radix Artemisia ordosicae, sorrel, Radix Sophorae Flavescentiss and Herba polygoni hydropiperis use Herb；Then,
Deep ploughing is digged, and pours permeable；5 days before Radix Notoginseng transplantation of seedlings, per mu is applied farm manure 3000kg, and intertillage is digged；
S3, seed are selected
The full mellow fruit tied by 3 years above Radix Notoginseng plant of life is chosen, is rubbed with the hands and peel is removed, clean, obtain Radix Notoginseng kind
Son；
S4, Radix Notoginseng nursery
1) culture medium prepares：Callus inducing medium：B5+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L KT, adjusts pH 5.8；Calluss division culture medium：MS+1.5mg/L 2,4-D+0.1mg/L
IBA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamycin, adjusts pH 6.0；Root induction culture medium：MS+
1.0mg/L NAA+1.0mg/L IBA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3, adjusts pH 5.8；
2) explant prepares：Take the Panax notoginseng seeds that chooses in step S3 and explant is done, by Panax notoginseng seeds in 75% ethanol
60s is soaked, then in 0.1%HgCl230min is soaked in solution, with aseptic water washing 5～8 times；Then, Panax notoginseng seeds are peelled off
Kind skin；
3) it is inoculated with：The Panax notoginseng seeds for peelling off kind of skin are inoculated on callus culture, light culture 5 under conditions of 15 DEG C
My god, then cultivate 36 days under conditions of 15 DEG C, the daily illumination of 1900Lx 12 hours, obtain calluss；
4) successive transfer culture:Calluss are transferred on calluss division culture medium, in 15 DEG C, the daily illumination of 2000Lx
Under conditions of 8 hours, once, proliferation times are 1 to 15 days subcultures:10, obtain Regenerated plant；
5) root culture：Regenerated plant is transferred in root induction culture medium, in 15 DEG C, the daily illumination of 2000Lx 10 hours
Under conditions of cultivate 15 days, seedling of must taking root；
6) seedling exercising：Seedling of taking root is taken out from culture medium, clean root culture medium, plants the sand soil seedbed in greenhouse
In, natural lighting, seedling exercising 8 days, obtain Radix Notoginseng seedling；
S5, transplanting
Radix Notoginseng seedling root is dipped after trichoderma harzianum bacterium solution of the concentration for 150g/L, is put in order in transplanting to S2 step
In forest land, spacing in the rows 30cm, line-spacing 40cm, per mu is applied azophoska 15kg, and the constituent weight ratio of the azophoska is to urinate
Element:Calcium superphosphate:Potassium sulfate=2:1:3, root water is poured, and with the ClO 2 solution that volume ratio is 1%, earth's surface is carried out
Spray sterilization；
S6, growth management
First Year May applies calcium magnesium phosphate and potash fertilizer, with per plant of the trichoderma harzianum bacterium solution that concentration is 50g/L after applying fertilizer one week
200mL pouring root；
Second Year and the 3rd year beginning of spring, to notoginseng planting per mu spray concentration for 30g/L trichoderma harzianum bacterium solution
100L, and per mu applied farm manure 3000kg, the weight ratio of farm manure constituent is, sheep stool:Cattle manure:Pig manure:Chicken manure=2:2:
5:1；In 4～June potassium application, it is per plant of 200mL pouring root of 50g/L trichoderma harzianum bacterium solution in May concentration；
In Second Year June, flower of Radix Notoginseng a kind of sedge is beaten and remove；November to notoginseng planting per mu spread fertilizer over the fields plant ash 1000kg；
7～August plucks flower of Radix Notoginseng within 3rd year, then the Adlerika of foliage-spray 10g/L, and being sprayed onto blade face dropping liquid is
Only；
Find that weeds remove in time in whole growth course；Discovery has disease strain to pull out in time；
S7, harvesting
In late October, the 3rd to early November, Radix Notoginseng is excavated when turning withered and yellow by Radix Notoginseng cane, removes stem and leaf and soil, shines
Dry preservation.
Embodiment 2
A kind of implantation methods of Radix Notoginseng, comprise the steps：
S1, selection planting site
Select that the soil is porous is fertile, the forest land of the plantation Folium Eriobotryae of well-drained neutral or slant acidity sand loam, and should
The light transmittance in forest land is 30%；
S2, site preparation
In the Radix Notoginseng transplantation of seedlings previous moon, per mu apply quick lime 250kg, Herba Artemisiae Annuae 300kg, Radix Artemisia ordosicae 150kg, Bulbus Allii 20kg,
Rheum officinale 50kg, Radix Sophorae Flavescentiss 70kg, Herba polygoni hydropiperis 70kg and Pericarpium Zanthoxyli 10kg, quick lime, Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Rheum officinale, Radix Sophorae Flavescentiss,
Herba polygoni hydropiperis and Pericarpium Zanthoxyli are to apply forest land after crushed, and Herba Artemisiae Annuae, Radix Artemisia ordosicae, Rheum officinale, Radix Sophorae Flavescentiss and Herba polygoni hydropiperis use Herb；Then,
Deep ploughing is digged, and pours permeable；5 days before Radix Notoginseng transplantation of seedlings, per mu is applied farm manure 5000kg, and intertillage is digged；
S3, seed are selected
The full mellow fruit tied by 3 years above Radix Notoginseng plant of life is chosen, is rubbed with the hands and peel is removed, clean, obtain Radix Notoginseng kind
Son；
S4, Radix Notoginseng nursery
1) culture medium prepares：Callus inducing medium：B5+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L KT, adjusts pH 6.0；Calluss division culture medium：MS+1.5mg/L 2,4-D+0.1mg/L
IBA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamycin, adjusts pH 6.3；Root induction culture medium：MS+
1.0mg/L NAA+1.0mg/L IBA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3, adjusts pH 5.6；
2) explant prepares：Take the Panax notoginseng seeds that chooses in step S3 and explant is done, by Panax notoginseng seeds in 75% ethanol
60s is soaked, then in 0.1%HgCl220min is soaked in solution, with aseptic water washing 5～8 times；Then, Panax notoginseng seeds are peelled off
Kind skin；
3) it is inoculated with：The Panax notoginseng seeds for peelling off kind of skin are inoculated on callus culture, light culture 3 under conditions of 20 DEG C
My god, then cultivate 30 days under conditions of 20 DEG C, the daily illumination of 1600Lx 12 hours, obtain calluss；
4) successive transfer culture:Calluss are transferred on calluss division culture medium, in 20 DEG C, the daily illumination of 1800Lx
Under conditions of 10 hours, once, proliferation times are 1 to 20 days subcultures:8, obtain Regenerated plant；
5) root culture：Regenerated plant is transferred in root induction culture medium, in 20 DEG C, the daily illumination of 1800Lx 8 hours
Under conditions of cultivate 10 days, seedling of must taking root；
6) seedling exercising：Seedling of taking root is taken out from culture medium, clean root culture medium, plants the sand soil seedbed in greenhouse
In, natural lighting, seedling exercising 5 days, obtain Radix Notoginseng seedling；
S5, transplanting
Radix Notoginseng seedling root is dipped after trichoderma harzianum bacterium solution of the concentration for 200g/L, is put in order in transplanting to S2 step
In forest land, spacing in the rows 30cm, line-spacing 40cm, per mu is applied azophoska 15kg, and the constituent weight ratio of the azophoska is to urinate
Element:Calcium superphosphate:Potassium sulfate=2:1:3, root water is poured, and with the ClO 2 solution that volume ratio is 1%, earth's surface is carried out
Spray sterilization；
S6, growth management
First Year May applies calcium magnesium phosphate and potash fertilizer, with per plant of the trichoderma harzianum bacterium solution that concentration is 50g/L after applying fertilizer one week
200mL pouring root；
Second Year and the 3rd year beginning of spring, to notoginseng planting per mu spray concentration for 30g/L trichoderma harzianum bacterium solution
100L, and per mu applied farm manure 3000kg, the weight ratio of farm manure constituent is, sheep stool:Cattle manure:Pig manure:Chicken manure=2:2:
5:1；In 4～June potassium application, it is per plant of 200mL pouring root of 50g/L trichoderma harzianum bacterium solution in May concentration；
In Second Year June, flower of Radix Notoginseng a kind of sedge is beaten and remove；November to notoginseng planting per mu spread fertilizer over the fields plant ash 1500kg；
7～August plucks flower of Radix Notoginseng within 3rd year, and then the Adlerika of foliage-spray 10g/L, sprays to blade face dropping liquid；
Find that weeds remove in time in whole growth course；Discovery has disease strain to pull out in time；
S7, harvesting
In late October, the 3rd to early November, Radix Notoginseng is excavated when turning withered and yellow by Radix Notoginseng cane, removes stem and leaf and soil, shines
Dry preservation.
Embodiment 3
A kind of implantation methods of Radix Notoginseng, comprise the steps：
S1, selection planting site
Select that the soil is porous is fertile, the forest land of the plantation Fructus Citri grandiss of well-drained neutral or slant acidity sand loam, and should
The light transmittance in forest land is 30%；
S2, site preparation
In the Radix Notoginseng transplantation of seedlings previous moon, per mu apply quick lime 230kg, Herba Artemisiae Annuae 380kg, Radix Artemisia ordosicae 100kg, Bulbus Allii 15kg,
Rheum tanguticum 70kg, Radix Sophorae Flavescentiss 60kg, Herba polygoni hydropiperis 50kg and Pericarpium Zanthoxyli 20kg, quick lime, Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Rheum tanguticum,
Radix Sophorae Flavescentiss, Herba polygoni hydropiperis and Pericarpium Zanthoxyli are to apply forest land after crushed, and Herba Artemisiae Annuae, Radix Artemisia ordosicae, Rheum tanguticum, Radix Sophorae Flavescentiss and Herba polygoni hydropiperis are used entirely
Strain；Then, deep ploughing is digged, and pours permeable；5 days before Radix Notoginseng transplantation of seedlings, per mu is applied farm manure 4200kg, and intertillage is digged；
S3, seed are selected
The full mellow fruit tied by 3 years above Radix Notoginseng plant of life is chosen, is rubbed with the hands and peel is removed, clean, obtain Radix Notoginseng kind
Son；
S4, Radix Notoginseng nursery
1) culture medium prepares：Callus inducing medium：B5+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L KT, adjusts pH 5.6；Calluss division culture medium：MS+1.5mg/L 2,4-D+0.1mg/L
IBA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamycin, adjusts pH 5.8；Root induction culture medium：MS+
1.0mg/L NAA+1.0mg/L IBA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3, adjusts pH 6.4；
2) explant prepares：Take the Panax notoginseng seeds that chooses in step S3 and explant is done, by Panax notoginseng seeds in 75% ethanol
60s is soaked, then in 0.1%HgCl225min is soaked in solution, with aseptic water washing 5～8 times；Then, Panax notoginseng seeds are peelled off
Kind skin；
3) it is inoculated with：The Panax notoginseng seeds for peelling off kind of skin are inoculated on callus culture, light culture 5 under conditions of 10 DEG C
My god, then cultivate 40 days under conditions of 10 DEG C, the daily illumination of 1800Lx 12 hours, obtain calluss；
4) successive transfer culture:Calluss are transferred on calluss division culture medium, in 15 DEG C, the daily illumination of 1900Lx
Under conditions of 12 hours, once, proliferation times are 1 to 20 days subcultures:8, obtain Regenerated plant；
5) root culture：Regenerated plant is transferred in root induction culture medium, in 20 DEG C, the daily illumination of 1800Lx 12 hours
Under conditions of cultivate 14 days, seedling of must taking root；
6) seedling exercising：Seedling of taking root is taken out from culture medium, clean root culture medium, plants the sand soil seedbed in greenhouse
In, natural lighting, seedling exercising 5 days, obtain Radix Notoginseng seedling；
S5, transplanting
Radix Notoginseng seedling root is dipped after trichoderma harzianum bacterium solution of the concentration for 180g/L, is put in order in transplanting to S2 step
In forest land, spacing in the rows 30cm, line-spacing 40cm, per mu is applied azophoska 10kg, and the constituent weight ratio of the azophoska is to urinate
Element:Calcium superphosphate:Potassium sulfate=2:1:3, root water is poured, and with the ClO 2 solution that volume ratio is 1%, earth's surface is carried out
Spray sterilization；
S6, growth management
First Year May applies calcium magnesium phosphate and potash fertilizer, with per plant of the trichoderma harzianum bacterium solution that concentration is 50g/L after applying fertilizer one week
200mL pouring root；
Second Year and the 3rd year beginning of spring, to notoginseng planting per mu spray concentration for 30g/L trichoderma harzianum bacterium solution
100L, and per mu applied farm manure 3000kg, the weight ratio of farm manure constituent is, sheep stool:Cattle manure:Pig manure:Chicken manure=2:2:
5:1；In 4～June potassium application, it is per plant of 200mL pouring root of 50g/L trichoderma harzianum bacterium solution in May concentration；
In Second Year June, flower of Radix Notoginseng a kind of sedge is beaten and remove；November to notoginseng planting per mu spread fertilizer over the fields plant ash 1500kg；
7～August plucks flower of Radix Notoginseng within 3rd year, and then the Adlerika of foliage-spray 10g/L, sprays to blade face dropping liquid；
Find that weeds remove in time in whole growth course；Discovery has disease strain to pull out in time；
S7, harvesting
In late October, the 3rd to early November, Radix Notoginseng is excavated when turning withered and yellow by Radix Notoginseng cane, removes stem and leaf and soil, shines
Dry preservation.
Test example
1 ten thousand plant Radix Notoginseng are planted in Lijiang Yunnan respectively by the method for embodiment 1 to 3 within 2013, while arranging control
Group.Matched group is using conventional seedbed system method, does not use tissue culture, does not use Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentiss, peppery
Knotweed and Pericarpium Zanthoxyli are processed to forest land, also do not use trichoderma harzianum bacterium solution to carry out root dipping, pouring root to Radix Notoginseng and spray process.?
Nursery emergence rate is counted during nursery in 2013, and before in October, 2015 harvesting, statistics Radix Notoginseng survives strain rate.Statistical result is shown in Table 1.
1, table is tested gained Radix Notoginseng emergence rate, survives strain rate and notoginseng root rot strain rate
As can be seen from the above table, the emergence rate of embodiment 1 to 3 and survival rate are all significantly higher than matched group, embodiment 1 to 3
Root-rot diseased plant rate be substantially less than matched group.Using tissue culture, the emergence rate of Radix Notoginseng is improve；Using Herba Artemisiae Annuae, Radix Artemisia ordosicae, big
Bulbus Allii, Radix Et Rhizoma Rhei, Radix Sophorae Flavescentiss, Herba polygoni hydropiperis and Pericarpium Zanthoxyli are processed to forest land, and carry out root dipping, filling using trichoderma harzianum bacterium solution to Radix Notoginseng
Root and process is sprayed, improve Radix Notoginseng and survive strain rate, notoginseng root rot strain rate is reduced, solve notoginseng obstacle.
Above embodiments of the present invention are explained in detail, but the invention is not restricted to described embodiment.Right
For those skilled in the art, in the case of without departing from the principle of the invention and spirit, these embodiments are carried out many
Plant change, modification, replace and modification, still fall within protection scope of the present invention.
Claims (7)
1. a kind of implantation methods of Radix Notoginseng, it is characterised in that comprise the steps：
S1, selection planting site
Select that the soil is porous is fertile, well-drained neutral or slant acidity sand loam forest land, and the light transmittance in the forest land be
～30%；
S2, site preparation
In the Radix Notoginseng transplantation of seedlings previous moon, per mu apply 200～250kg of quick lime, 300～400kg of Herba Artemisiae Annuae, Radix Artemisia ordosicae 100～
150kg, 10～20kg of Bulbus Allii, 50～80kg of Radix Et Rhizoma Rhei, 50～70kg of Radix Sophorae Flavescentiss, 40～70kg of Herba polygoni hydropiperis and 10～30kg of Pericarpium Zanthoxyli, deep ploughing
Dig；5 days before Radix Notoginseng transplantation of seedlings, per mu is applied 3000～5000kg of farm manure, and intertillage is digged；
S3, seed are selected
The full mellow fruit tied by 3 years above Radix Notoginseng plant of life is chosen, is rubbed with the hands and peel is removed, clean, obtain Panax notoginseng seeds；
S4, Radix Notoginseng nursery
1) culture medium prepares：Callus inducing medium：B5+1.0mg/L NAA+1.0mg/L 6-BA+3g/L sucrose+
5.0g/L agar+0.2mg/L KT, adjusts pH 5.6～6.0；Calluss division culture medium：MS+1.5mg/L 2,4-D+
0.1mg/LIBA+3g/L sucrose+5.0g/L agar+0.5mg/L PVP+5mg/L gentamycin, adjusts pH 5.6～6.5；Take root and lure
Lead culture medium：MS+1.0mg/L NAA+1.0mg/L IBA+3g/L sucrose+5.0g/L agar+0.05mg/L GA3, adjusts pH 5.6
～6.5；
2) explant prepares：Take the Panax notoginseng seeds that chooses in step S3 and explant is done, Panax notoginseng seeds are soaked in 75% ethanol
60s, then in 0.1%HgCl220～30min is soaked in solution, with aseptic water washing 5～8 times；Then, Panax notoginseng seeds are peelled off
Kind skin；
3) it is inoculated with：The Panax notoginseng seeds for peelling off kind of skin are inoculated on callus culture, light culture 3 under conditions of 10～20 DEG C
～5 days, then cultivate 30～40 days under conditions of 10～20 DEG C, the daily illumination of 1600～1900Lx 12 hours, obtain wound healing group
Knit；
4) successive transfer culture:Calluss are transferred on calluss division culture medium, 10～20 DEG C, 1800～2000Lx per
Under conditions of its illumination 8～12 hours 15～20 days subcultures once, proliferation times be:8～10, obtain Regenerated plant；
5) root culture：Regenerated plant is transferred in root induction culture medium, in 15～20 DEG C, the daily illumination of 1800～2000Lx
Cultivate 10～15 days under conditions of 8～12 hours, seedling of must taking root；
6) seedling exercising：Seedling of taking root is taken out from culture medium, clean root culture medium, is planted in the sand soil seedbed in greenhouse,
Natural lighting, seedling exercising 5～8 days, obtain Radix Notoginseng seedling；
S5, transplanting
Radix Notoginseng seedling root is dipped after trichoderma harzianum bacterium solution, in the forest land that puts in order in transplanting to S2 step, spacing in the rows 30cm, OK
Away from 40cm, per mu is applied 10～15kg of azophoska, and the constituent weight ratio of the azophoska is, carbamide:Calcium superphosphate:Sulfur
Sour potassium=2:1:3, root water is poured, and with the ClO 2 solution that volume ratio is 1%, earth's surface is carried out spraying sterilization；
S6, growth management
First Year May applies calcium magnesium phosphate and potash fertilizer, and fertilising used trichoderma harzianum bacterium solution pouring root after one week；
Second Year and the 3rd year beginning of spring, spray trichoderma harzianum bacterium solution to notoginseng planting, and per mu apply farm manure 2000～
3000kg, the weight ratio of the farm manure constituent is, sheep stool:Cattle manure:Pig manure:Chicken manure=2:2:5:1；Potassium is applied in 4～June
Fertilizer, in May with trichoderma harzianum bacterium solution pouring root；
In Second Year June, flower of Radix Notoginseng a kind of sedge is beaten and remove；November to notoginseng planting per mu spread fertilizer over the fields 1000～1500kg of plant ash；
7～August plucks flower of Radix Notoginseng within 3rd year, then imposes fertiliser containing magnesium；
Find that weeds remove in time in whole growth course；Discovery has disease strain to pull out in time；
S7, harvesting
In late October, the 3rd to early November, Radix Notoginseng is excavated when turning withered and yellow by Radix Notoginseng cane, removes stem and leaf and soil, dries guarantor
Deposit.
2. implantation methods of Radix Notoginseng according to claim 1, it is characterised in that the Herba Artemisiae Annuae, Radix Artemisia ordosicae, Bulbus Allii, Radix Et Rhizoma Rhei, hardship
Ginseng, Herba polygoni hydropiperis and Pericarpium Zanthoxyli apply forest land after crushed.
3. implantation methods of Radix Notoginseng according to claim 1 and 2, it is characterised in that the Radix Et Rhizoma Rhei is sorrel, medicinal
Radix Et Rhizoma Rhei or the Herb of Rheum tanguticum.
4. implantation methods of Radix Notoginseng according to claim 1, it is characterised in that apply fertiliser containing magnesium for blade face described in step S6
The Adlerika of 10g/L is sprayed, is sprayed to blade face dropping liquid.
5. implantation methods of Radix Notoginseng according to claim 1, it is characterised in that the trichoderma harzianum bacterium described in step S5
The concentration of liquid is 150～200g/L.
6. implantation methods of Radix Notoginseng according to claim 1, it is characterised in that in step S6, described for pouring root
The concentration of trichoderma harzianum bacterium solution is 50g/L, and per plant of Radix Notoginseng irrigates 200mL；Described for the trichoderma harzianum bacterium solution that sprays
Concentration is 30g/L, and per mu sprays 100L.
7. implantation methods of the Radix Notoginseng according to any one of claim 1～6, it is characterised in that the forest land is plantation oil
Fructus Canarii albi, Semen Juglandiss, Folium Eriobotryae, the field of Fructus Vitis viniferae or Fructus Citri grandiss.
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| CN108934733A (en) * | 2018-09-19 | 2018-12-07 | 沛县新太阳野生蔬菜种植专业合作社 | A method of Gynura procumbens (Lour.) Merr is planted based on grape forest land |
| CN109964770A (en) * | 2019-03-21 | 2019-07-05 | 文山高田三七中药饮片有限公司 | A kind of implantation methods improving notoginseng obstacle |
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