CN106386312B - Method for breeding seeds of rice by two-line sterile line - Google Patents
Method for breeding seeds of rice by two-line sterile line Download PDFInfo
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- CN106386312B CN106386312B CN201610828040.4A CN201610828040A CN106386312B CN 106386312 B CN106386312 B CN 106386312B CN 201610828040 A CN201610828040 A CN 201610828040A CN 106386312 B CN106386312 B CN 106386312B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a method for breeding seeds of two-line sterile lines of rice, which comprises the steps of selecting a proper planting area according to the fertility conversion characteristics of the two-line sterile lines, and determining the seed sowing time according to the growth period; the sterile line plant of the seed production can be harvested when the seed is in the late stage of wax ripening and the early stage of complete ripening, and the height of mechanized harvesting is adjusted according to the local seasonal characteristics; after harvesting the male parent for seed production, herbicide is sprayed to ensure that normal regeneration, ear sprouting and fructification cannot be continued; fertilizing and watering the sterile rice piles after seed production, and harvesting seeds until the seeds are in the mature period; according to the invention, the hybrid rice sterile line breeding and seed production are carried out in different seasons of the same region, according to the temperature-sensitive characteristic and strong regeneration capability of the two-line sterile line, after the seed production mission is completed in the high-temperature season of summer by the two-line sterile line, the two-line sterile line is continuously regenerated and grown in a rice pile remaining mode, the breeding mission is completed in the low-temperature season of autumn, the complex flow of breeding and seed production in different places is saved, the seed production link is reduced, and the seed production cost is reduced.
Description
Technical Field
The invention belongs to the technical field of crop breeding, and particularly relates to a method for breeding seeds of a two-line sterile line of rice.
Background
The two-line hybrid rice is a new technology which is firstly researched and succeeded in China in the world and utilizes the heterosis of rice. The two-line method for utilizing the rice heterosis simplifies the procedures of propagation and seed production, reduces the links of seed production and lowers the production cost of seeds. The successful breeding of the hybrid rice by the two-line method opens up a new way for the utilization of the heterosis of the rice.
The two-line hybrid rice seed production has female parent of photo-thermo-sensitive genic male sterile line, sterility expression controlled by both cell nucleus gene and ecological condition, complete expression of male sterility and high purity of the produced hybrid rice. Therefore, in the two-line hybrid rice seed production, the seed purity and the yield are both main targets, the purity is a precondition, and the yield is only obtained on the basis of ensuring the seed production purity, which has practical significance. In order to ensure that the seed purity of the two-line hybrid rice seed production is qualified, the season arrangement and the selection of the base have stricter requirements. In the three-line hybrid rice breeding, as the sterility of the female parent is not restricted by environmental conditions, the fertility sensitive safe period of the sterile line does not need to be considered, and only the safe period of the heading pollination needs to be considered. The two-line hybrid rice breeding is different, the expression of the female sterility needs specific light and temperature conditions, the selection of seed breeding base and the arrangement of seasons, the safety of the fertility sensitive period of the sterile line and the safety of the heading pollination period need to be considered, wherein the fertility sensitive safety period is used as a precondition. Because of this, the breeding of the sterile line of the two-line hybrid rice in China is mostly concentrated on the spring breeding in Hainan, and the seed production is mainly concentrated on the summer in the lower reaches of Yangtze river, thus greatly increasing the production cost.
Disclosure of Invention
The invention aims to provide a method for breeding seeds of a two-line sterile line of rice, and aims to solve the problems of complicated seed production process and high seed production cost of the existing allopatric breeding.
The invention is realized in this way, a method for breeding seeds of two-line sterile line of rice, which comprises the following steps:
selecting a proper planting area according to the fertility conversion characteristics of the selected two-line sterile line, and determining the sowing time of the two-line sterile line according to the growth period so that the temperature of the safe sterile sensitive period of the sterile line is higher than the fertility conversion initial temperature;
step two, the sterile line plants of the seed production can be harvested when the seeds are in the late stage of the wax ripeness and the early stage of the complete ripeness, and the height of mechanized harvesting is adjusted according to the local seasonal characteristics;
step three, killing the male parent after harvesting with 200 g of 10% glyphosate solution per mu, so that normal regeneration, ear sprouting and fruit setting cannot be continued;
irrigating the sterile rice stakes after seed production, and applying 40 kg of compound fertilizer per mu to promote tillering regeneration of the field as soon as possible so as to achieve strong seedlings;
and step five, the seeds are harvested until the mature period.
Further, in the second step, the sterile line plants of the seed production can be harvested when the seeds are in the late stage of wax ripening and the early stage of complete ripening, and when the height of mechanized harvesting is adjusted according to the local seasonal characteristics, if the temperature accumulation is relatively less after the sterile line is harvested and the temperature drop speed is high, the sterile line is harvested from the reverse section or the reverse two sections, so that the sterile line can be spilt and fruit as early as possible; if the temperature is relatively more and the temperature drop speed is slow after the sterile line is harvested, the sterile line is harvested from the inverted three-section or four-section part, so that the sterile line delays the heading and fructification.
The method for breeding seeds of two-line sterile lines of rice provided by the invention selects a proper planting area according to the fertility conversion characteristics of the selected two-line sterile lines, and determines the sowing time of the two-line sterile lines according to the growth period, so that the temperature of the safe sterile sensitive period of the sterile lines is higher than the initial temperature of fertility conversion; the sterile line plant of the seed production can be harvested when the seed is in the late stage of wax ripening and the early stage of complete ripening, and the height of mechanized harvesting is adjusted according to the local seasonal characteristics; after harvesting the male parent of the seed production, herbicide is sprayed to ensure that normal regeneration, heading and fructification cannot be continued; fertilizing and watering the sterile rice stake after seed production to promote the multi-tillering regeneration as soon as possible, so as to achieve strong seedling; harvesting the seeds until the mature period; according to the method, the breeding and seed production of the hybrid rice sterile line are carried out in different seasons in the same region, according to the temperature-sensitive characteristic and strong regeneration capacity of the two-line sterile line, after the seed production mission is finished in the high-temperature season in summer, the two-line sterile line is continuously regenerated and grown in a rice pile remaining mode, the breeding mission is finished in the low-temperature season in autumn, the complex flow of breeding and seed production in different places is saved, the seed production link is reduced, and the seed production cost is reduced.
Drawings
FIG. 1 is a flow chart of the implementation of the method for breeding seeds of two-line sterile line of rice according to the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in figure 1, the invention is realized by a method for breeding and producing seeds of a two-line sterile line of rice, which comprises the following steps:
step S101, selecting a proper planting area according to the fertility conversion characteristics of the selected two-line sterile line, and determining the sowing time of the two-line sterile line according to the growth period so that the temperature of the safe sterility sensitive period of the sterile line is higher than the fertility conversion initial temperature;
s102, harvesting sterile plants in the late stage of wax ripening and the early stage of complete ripening of the seeds, and adjusting the height of mechanized harvesting according to the local seasonal characteristics;
step S103, killing the male parent after harvesting with 200 g of 10% glyphosate solution per mu, so that normal regeneration, ear sprouting and fruit setting cannot be continued;
step S104, irrigating the sterile rice stakes after seed production, and applying 40 kilograms of compound fertilizer per mu to promote tillering regeneration of multiple tillers as soon as possible so as to achieve strong seedlings;
and S105, waiting for seeds to be harvested until the mature period.
In the embodiment of the invention, in step S102, the sterile line plants for seed production can be harvested when the seeds are in the late stage of waxiness and the early stage of maturity, and when the height of mechanized harvesting is adjusted according to the local seasonal characteristics, if the accumulated temperature is relatively less after the sterile line is harvested and the temperature drop speed is high, the sterile line is harvested from the position of the reverse section or the reverse section, so that the sterile line can be spilt and fruit as soon as possible; if the temperature is relatively more and the temperature drop speed is slow after the sterile line is harvested, the sterile line is harvested from the inverted three-section or four-section part, so that the sterile line delays the heading and fructification.
The application of the principles of the present invention will be further described with reference to the accompanying drawings and specific embodiments.
Example 1:
1. and selecting a proper planting area according to the fertility conversion characteristics of the selected two-line sterile line, and determining the sowing time of the seeds according to the growth period of the two-line sterile line to ensure that the temperature of the sterile line in the safe sterile sensitive period is higher than the initial temperature of the fertility conversion.
2. The sterile line plant of the seed production can be harvested when the seed is in the late stage of wax ripening and the early stage of complete ripening, and the harvesting is to ensure the strong regeneration capability of the sterile line plant. The harvesting height is adjusted according to local seasonal characteristics (mainly temperature change) during mechanized harvesting, if the accumulated temperature is relatively low and the temperature drop speed is high after the sterile line is harvested in a certain place, the sterile line is harvested from a reverse section or a reverse section, so that the sterile line can be spiked and fructified as early as possible; if the temperature accumulation is relatively more and the temperature reduction speed is slow after the sterile line is harvested in a certain place, the sterile line is harvested from the inverted three sections or the inverted four sections, so that the sterile line can delay the heading and fructification.
3. After the male parent of the seed production is harvested, herbicide is sprayed to ensure that the male parent can not continue to normally regenerate, sprout and fruit.
4. Further fertilizing and watering the sterile rice stake after seed production to promote the multi-tillering regeneration as soon as possible, thereby achieving the purposes of seedling strengthening and seedling strengthening.
5. With the further reduction of the temperature, the grain filling of the sterile lines of the two lines is slower after the loose powder is fructified, and the seeds are harvested until the mature period after the tolerance waiting.
The method for breeding seeds of the two-line sterile line of rice provided by the embodiment of the invention selects a proper planting area according to the fertility conversion characteristics of the selected two-line sterile line, and determines the sowing time of the two-line sterile line according to the growth period, so that the temperature of the safe sterile sensitive period of the sterile line is higher than the initial temperature of fertility conversion; the sterile line plant of the seed production can be harvested when the seed is in the late stage of wax ripening and the early stage of complete ripening, and the height of mechanized harvesting is adjusted according to the local seasonal characteristics; after harvesting the male parent of the seed production, herbicide is sprayed to ensure that normal regeneration, heading and fructification cannot be continued; fertilizing and watering the sterile rice stake after seed production to promote the multi-tillering regeneration as soon as possible, so as to achieve strong seedling; harvesting the seeds until the mature period; according to the method, the breeding and seed production of the hybrid rice sterile line are carried out in different seasons in the same region, according to the temperature-sensitive characteristic and strong regeneration capacity of the two-line sterile line, after the seed production mission is finished in the high-temperature season in summer, the two-line sterile line is continuously regenerated and grown in a rice pile remaining mode, the breeding mission is finished in the low-temperature season in autumn, the complex flow of breeding and seed production in different places is saved, the seed production link is reduced, and the seed production cost is reduced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (1)
1. A method for breeding seeds of two-line sterile lines of rice is characterized by comprising the following steps:
selecting a proper planting area according to the fertility conversion characteristics of the selected two-line sterile line, and determining the sowing time of the two-line sterile line according to the growth period so that the temperature of the safe sterile sensitive period of the sterile line is higher than the fertility conversion initial temperature;
step two, the sterile line plants of the seed production can be harvested when the seeds are in the late stage of the wax ripeness and the early stage of the complete ripeness, and the height of mechanized harvesting is adjusted according to the local seasonal characteristics;
step three, killing the male parent after harvesting with 200 g of 10% glyphosate solution per mu, so that normal regeneration, ear sprouting and fruit setting cannot be continued;
irrigating the sterile rice stakes after seed production, and applying 40 kg of compound fertilizer per mu to promote tillering regeneration of the field as soon as possible so as to achieve strong seedlings;
fifthly, the seeds are harvested until the mature period;
in the second step, the sterile line plant of seed production can be harvested when the seed is in the late stage of wax ripening and the early stage of complete ripening, when the height of mechanized harvesting is adjusted according to the local season characteristics, if the accumulated temperature is relatively less after the sterile line is harvested and the temperature drop speed is high, the sterile line is harvested from the reverse section or the reverse section, so that the sterile line can be spilt and fruit as soon as possible; if the accumulated temperature is relatively more after the sterile line is harvested and the temperature reduction speed is slow, harvesting the sterile line from the inverted three sections or the inverted four sections, and delaying the sprouting and fructification of the sterile line;
according to the method for breeding and producing seeds of the two-line sterile line of the rice, the breeding and producing seeds of the sterile line of the hybrid rice are carried out in different seasons of the same region, and according to the temperature-sensitive characteristic and the strong regeneration capacity of the two-line sterile line, after the seed production mission of the two-line sterile line is completed in the high-temperature season of summer, the two-line sterile line is continuously regenerated and grown in a rice pile remaining mode, and the breeding mission is completed in the low-temperature season of autumn.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610828040.4A CN106386312B (en) | 2016-09-18 | 2016-09-18 | Method for breeding seeds of rice by two-line sterile line |
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| CN201610828040.4A CN106386312B (en) | 2016-09-18 | 2016-09-18 | Method for breeding seeds of rice by two-line sterile line |
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| CN106857132A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of simple two-line sterile line propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055098A (en) * | 1990-03-29 | 1991-10-09 | 福建农学院 | The improved route of two-line hybrid rice |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102771384A (en) * | 2012-08-22 | 2012-11-14 | 江苏丰源种业有限公司 | Method for seed production of two-line hybrid rice |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
-
2016
- 2016-09-18 CN CN201610828040.4A patent/CN106386312B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055098A (en) * | 1990-03-29 | 1991-10-09 | 福建农学院 | The improved route of two-line hybrid rice |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102771384A (en) * | 2012-08-22 | 2012-11-14 | 江苏丰源种业有限公司 | Method for seed production of two-line hybrid rice |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
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