CN106386312A - Method for propagation and seed production of two-line sterile lines of rice - Google Patents
Method for propagation and seed production of two-line sterile lines of rice Download PDFInfo
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- CN106386312A CN106386312A CN201610828040.4A CN201610828040A CN106386312A CN 106386312 A CN106386312 A CN 106386312A CN 201610828040 A CN201610828040 A CN 201610828040A CN 106386312 A CN106386312 A CN 106386312A
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- line
- sterile line
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- sterile
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for propagation and seed production of two-line sterile lines of rice. The method comprises the following steps of: selecting a proper planting area according to the characteristic of fertility alteration of the two-line sterile lines, and deciding the seed sowing time according to the growth period; harvesting when the seeds of sterile-line plants for seed production are at the late stage of wax ripeness and the initial stage of complete ripeness, and adjusting the height of mechanical harvesting according to the local seasonal characteristics; spraying a weed kill after male parents for seed production so as to prevent continuous and normal regeneration, heading and fruiting; and carrying out fertilization and watering on the rice stems of the sterile lines after seed production, and harvesting when the seeds are completely ripened. The method disclosed by the invention has the advantages that the propagation and seed production of the sterile lines of rice are carried out at the same area and in different seasons, and according to the temperature-sensitive characteristic and strong regeneration capability of the two-line sterile lines, the two-line sterile lines regenerate and grow continuously by a mode of remaining the rice stems after completing the task of seed production, and complete the task of propagation in the low-temperature season of autumn, so that the cumbersome process of remote propagation and seed production is omitted, the seed production link is reduced and the seed production cost is reduced.
Description
Technical field
The invention belongs to technical field of crop propagation, more particularly, to a kind of method of two-line sterile line of rice crossbreeding.
Background technology
Double-hybrid rice strains is that China studies a kind of successfully new skill of utilization rice heterosis in the world first
Art.Two line method rice heterosis utilization approach, simplifies breeding and production of hybrid seeds program, decreases seed production link, reduce
Seed production cost.The selection-breeding success of double-hybrid rice strains, is that rice heterosis utilization opens a new way again.
The production of hybrid seeds of double-hybrid rice strains, female parent is to utilize photo-thermo-sensitive genic male sterile line, and the expression of sterility is by nucleus
Gene and warm Photo-ecology condition co- controlling, only under the conditions of certain warm Photo-ecology, male sterility can be expressed completely,
The purity of the production of hybrid seeds can be guaranteed.Therefore, in the double-hybrid rice strains production of hybrid seeds, seed purity and yield are all main mesh
Mark, and purity is premise, only asks yield to be just of practical significance on the basis of ensureing seed production purity.In order to ensure two line method
The seed purity of breeding of hybrid rice is qualified, and production of hybrid seeds season arranges and the selection in base has tightened up requirement.Miscellaneous in three line method
Hand in Oryza sativa L. crossbreeding, because maternal sterility is not restricted by environmental condition, therefore the fertility without the concern for sterile line is quick
Feel Safe period, only need to consider the Safe period of pollination of earing.Double-hybrid rice strains crossbreeding is then different, its maternal sterility
Expression needs specific light and temperature condition, the arrangement selecting with season of seed breeding base, not only will consider sterile line fertility-sensitive period
Safety, consider the safety of heading pollination period again, condition wherein premised on fertility sensitive Safe period.Just because of this, I
The breeding of state's double-linear hybrid rice sterile line focuses mostly in the breeding of Hainan spring, and the production of hybrid seeds is concentrated mainly on the summer of THE LOWER YANGTZE VALLEY
Carry out, substantially increase production cost.
Content of the invention
It is an object of the invention to provide a kind of method of two-line sterile line of rice crossbreeding is it is intended to solve current strange land
The problem that crossbreeding flow process is loaded down with trivial details, seed production cost is higher.
The present invention is achieved in that a kind of method of two-line sterile line of rice crossbreeding, this two-line sterile line of rice
The method of crossbreeding comprises the following steps：
Step one, the Fertility al-teration feature according to the two-line sterile line selecting selects suitable planting area, according to fertility
Phase determines that the sowing time of two-line sterile line initiates temperature so that the safety of sterile line temperature of sterile sensitive period is higher than Fertility al-teration
Degree；
Step 2, the sterile line plant of the production of hybrid seeds is in late dough in seed, the complete ripeness initial stage can harvest, according to local
Seasonal characteristic adjusts the height of mechanized harvest；
Step 3, to every mu after the harvesting of the male parent of the production of hybrid seeds with 10% gyphosate solution 200 grams carry out going out except so that can not continue
Continuous regular regeneration heading is solid；
Step 4, irrigates to the sterile line rice stub after the production of hybrid seeds, and 40 kilograms of Mu Shi compound fertilizer, promotes many points as soon as possible
Tiller regenerates, and reaches the strong Seedling of Seedling and strengthens；
Step 5, treats seed until full ripe stage harvests.
Further, in step 2, the sterile line plant of the production of hybrid seeds is in late dough in seed, the complete ripeness initial stage can receive
Obtain, adjusted according to local seasonal characteristic mechanized harvest height when, if accumulated temperature is relatively on the low side after sterile line harvests, temperature
Decrease speed is fast, then sterile line, from one section or two section position harvesting, makes sterile line ear as early as possible solid；If receiving in sterile line
Obtain rear accumulated temperature relatively many, temperature drop speed is slow, then sterile line, from three sections or four section position harvesting, makes sterile line postpone
Heading is solid.
The method of the two-line sterile line of rice crossbreeding that the present invention provides, turns according to the fertility of the two-line sterile line selecting
Feature of changing selects suitable planting area, determines the sowing time of two-line sterile line so that the safety of sterile line according to period of duration
Sterile sensitive period temperature is higher than Fertility al-teration initial temperature；The sterile line plant of the production of hybrid seeds is at the beginning of seed is in late dough, complete ripeness
Phase can harvest, and adjust the height of mechanized harvest according to local seasonal characteristic；Play herbicide to after the male parent harvesting of the production of hybrid seeds,
So that it is solid to continue regular regeneration heading；To the sterile line rice stub fertilizing, watering after the production of hybrid seeds, promote many tillers as soon as possible again
Raw, reach the strong Seedling of Seedling and strengthen；Treat seed until full ripe stage harvests；The crossbreeding of sterile line of hybridized rice is placed on same by the present invention
The Various Seasonal in area is carried out, the temperature sensitive feature according to two-line sterile line and strong regeneration capacity, and two-line sterile line is in summer high temperature
After season completes production of hybrid seeds mission, two-line sterile line is allowed to continue regenerative growth by way of staying rice stub, low temperature season is complete in the fall
Become breeding mission, eliminate the loaded down with trivial details flow process of the relocation propagation production of hybrid seeds, decrease seed production link, reduce seed and produce into
This.
Brief description
Fig. 1 is the flowchart of the method for two-line sterile line of rice crossbreeding provided in an embodiment of the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
As shown in figure 1, the present invention is achieved in that a kind of method of two-line sterile line of rice crossbreeding, this Oryza sativa L.
The method of two-line sterile line crossbreeding comprises the following steps：
Step S101, the Fertility al-teration feature according to the two-line sterile line selecting selects suitable planting area, according to life
Phase of educating determines that the sowing time of two-line sterile line initiates temperature so that the safety of sterile line temperature of sterile sensitive period is higher than Fertility al-teration
Degree；
Step S102, the sterile line plant of the production of hybrid seeds is in late dough in seed, the complete ripeness initial stage can harvest, according to locality
Seasonal characteristic adjust mechanized harvest height；
Step S103, carries out going out with 200 grams of 10% gyphosate solution to every mu after the harvesting of the male parent of the production of hybrid seeds except so that can not
Continue regular regeneration heading solid；
Step S104, irrigates to the sterile line rice stub after the production of hybrid seeds, and 40 kilograms of Mu Shi compound fertilizer, promotes many as soon as possible
Tiller regenerates, and reaches the strong Seedling of Seedling and strengthens；
Step S105, treats seed until full ripe stage harvests.
In embodiments of the present invention, in step s 102, the sterile line plant of the production of hybrid seeds is in late dough, complete ripeness in seed
Initial stage can harvest, adjusted according to local seasonal characteristic mechanized harvest height when, if accumulated temperature phase after sterile line harvests
To on the low side, temperature drop speed is fast, then sterile line, from one section or two section positions harvesting, makes sterile line ear as early as possible solid；
If accumulated temperature is relatively many after sterile line harvests, temperature drop speed is slow, then sterile line gathers in from three sections or four section positions,
Sterile line is made to postpone heading solid.
Below in conjunction with the accompanying drawings and specific embodiment is further described to the application principle of the present invention.
Embodiment 1：
1. the Fertility al-teration feature according to the two-line sterile line selecting selects suitable planting area, is determined according to its period of duration
Determine the sowing time of seed it is ensured that the safety of sterile line temperature of sterile sensitive period is higher than its Fertility al-teration initial temperature.
2. the sterile line plant of the production of hybrid seeds is in late dough in seed, the complete ripeness initial stage can harvest, now harvest be for
Ensure the strong regeneration capacity of sterile line plant.According to local seasonal characteristic (mainly temperature change feelings during mechanized harvest
Condition) adjustment harvesting height, if somewhere accumulated temperature after sterile line results is relatively on the low side, temperature drop speed is fast, then sterile line from
A section or two section positions harvestings, such sterile line can be eared solid as early as possible；If somewhere accumulated temperature phase after sterile line results
To more, temperature drop speed is slow, then from three sections or four section positions harvesting, such sterile line can postpone heading to sterile line
Solid.
3. pair production of hybrid seeds male parent harvesting after play herbicide it is ensured that its can not continue regular regeneration ear solid.
4. the further fertilizing, watering of sterile line rice stub after pair production of hybrid seeds, promotes its many tiller regeneration as soon as possible, reaches Seedling
Qiang Miaozhuan.
5., with the reduction further of temperature, two-line sterile line is slower in the solid rear kernel grouting of loose powder, bear with
Seed was just harvested up to full ripe stage.
The method of two-line sterile line of rice crossbreeding provided in an embodiment of the present invention, according to the two-line sterile line selecting
Fertility al-teration feature selects suitable planting area, determines the sowing time of two-line sterile line so that sterile line according to period of duration
Safety temperature of sterile sensitive period be higher than Fertility al-teration initial temperature；The sterile line plant of the production of hybrid seeds seed be in late dough,
The complete ripeness initial stage can harvest, and adjust the height of mechanized harvest according to local seasonal characteristic；Beat to after the male parent harvesting of the production of hybrid seeds
Herbicide so that can not continue regular regeneration heading solid；To the sterile line rice stub fertilizing, watering after the production of hybrid seeds, promote many as soon as possible
Tiller regenerates, and reaches the strong Seedling of Seedling and strengthens；Treat seed until full ripe stage harvests；The crossbreeding of sterile line of hybridized rice is put by the present invention
Various Seasonal in areal is carried out, the temperature sensitive feature according to two-line sterile line and strong regeneration capacity, and two-line sterile line is in the summer
Season high temperature season complete production of hybrid seeds mission after, allowed by way of staying rice stub two-line sterile line continue regenerative growth, low temperature in the fall
Season completes to breed mission, eliminates the loaded down with trivial details flow process of the relocation propagation production of hybrid seeds, decreases seed production link, reduces seed life
Produce cost.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (2)
1. a kind of method of two-line sterile line of rice crossbreeding is it is characterised in that this two-line sterile line of rice crossbreeding
Method comprises the following steps：
Step one, the Fertility al-teration feature according to the two-line sterile line selecting selects suitable planting area, is determined according to period of duration
Determine the sowing time of two-line sterile line so that the safety of sterile line temperature of sterile sensitive period is higher than Fertility al-teration initial temperature；
Step 2, the sterile line plant of the production of hybrid seeds is in late dough in seed, the complete ripeness initial stage can harvest, according to local season
Feature adjusts the height of mechanized harvest；
Step 3, to every mu after the harvesting of the male parent of the production of hybrid seeds with 10% gyphosate solution 200 grams carry out going out except so that just can not continue
Often regeneration heading is solid；
Step 4, irrigates to the sterile line rice stub after the production of hybrid seeds, and 40 kilograms of Mu Shi compound fertilizer, promotes many tillers as soon as possible again
Raw, reach the strong Seedling of Seedling and strengthen；
Step 5, treats seed until full ripe stage harvests.
2. the method for two-line sterile line of rice crossbreeding as claimed in claim 1 is it is characterised in that in step 2, make
Kind sterile line plant be in late dough in seed, the complete ripeness initial stage can harvest, and adjusts machinery according to local seasonal characteristic
Change harvesting height when, if accumulated temperature is relatively on the low side after sterile line harvests, temperature drop speed is fast, then sterile line from one section or
Two section positions harvestings, makes sterile line ear as early as possible solid；If after sterile line harvests, accumulated temperature is relatively many, temperature drop speed
Slowly, then sterile line, from three sections or four section position harvesting, makes sterile line postpone heading solid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610828040.4A CN106386312B (en) | 2016-09-18 | 2016-09-18 | Method for breeding seeds of rice by two-line sterile line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610828040.4A CN106386312B (en) | 2016-09-18 | 2016-09-18 | Method for breeding seeds of rice by two-line sterile line |
Publications (2)
| Publication Number | Publication Date |
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| CN106386312A true CN106386312A (en) | 2017-02-15 |
| CN106386312B CN106386312B (en) | 2020-01-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610828040.4A Expired - Fee Related CN106386312B (en) | 2016-09-18 | 2016-09-18 | Method for breeding seeds of rice by two-line sterile line |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857132A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of simple two-line sterile line propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055098A (en) * | 1990-03-29 | 1991-10-09 | 福建农学院 | The improved route of two-line hybrid rice |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102771384A (en) * | 2012-08-22 | 2012-11-14 | 江苏丰源种业有限公司 | Method for seed production of two-line hybrid rice |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
-
2016
- 2016-09-18 CN CN201610828040.4A patent/CN106386312B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055098A (en) * | 1990-03-29 | 1991-10-09 | 福建农学院 | The improved route of two-line hybrid rice |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN102119640A (en) * | 2010-11-19 | 2011-07-13 | 湖南农业大学 | Method for producing rice dual-purpose nucleic sterile line original seed |
| CN102771384A (en) * | 2012-08-22 | 2012-11-14 | 江苏丰源种业有限公司 | Method for seed production of two-line hybrid rice |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857132A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of simple two-line sterile line propagation method |
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| CN106386312B (en) | 2020-01-03 |
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