CN106857132A - A kind of simple two-line sterile line propagation method - Google Patents
A kind of simple two-line sterile line propagation method Download PDFInfo
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- CN106857132A CN106857132A CN201710172011.1A CN201710172011A CN106857132A CN 106857132 A CN106857132 A CN 106857132A CN 201710172011 A CN201710172011 A CN 201710172011A CN 106857132 A CN106857132 A CN 106857132A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of simple two-line sterile line propagation method, comprise the following steps：Under the weather conditions of Changsha, two-line sterile line seed is sowed in April, transplanted in May, sterile line strain number is expanded by point root and stem of certain plants in 6 July, and can be with restorer combo, in July, heading is bloomed, and carries out artificial supplementary pollination completion hybridization, and hybrid seed has been harvested in left and right maturation by the end of August；When harvesting seed, tassel can be first sliced off, retain the standing grain root and stem of certain plants；Irrigated using alternation of wetting and drying, anti-to cure pest and disease damage, temperature is gradually reduced after September part, and sterile line can complete Fertility al-teration, in 9 October neat fringe, or so November can harvest the male-sterile seed of breeding, and setting percentage can reach 50 80% or so.A kind of simple two-line sterile line propagation method that the present invention is provided, it is simple and practical, it is only necessary to get hold of and cut fringe and stay the root and stem of certain plants and flowering time, using the condition of later stage temperature reduction, two-line sterile line line of breeding can be realized.
Description
Technical field
The present invention relates to rice breeding field, especially a kind of simple two-line sterile line propagation method.
Background technology
Hubei stone finds photoperiod-temperature sensitive genie male-sterile line material Nongken 58S for bright loose 1973 and its effectively utilizes rice in China hybrid
Use of advantage opens frontier --- and double-hybrid rice strains, it was achieved huge by the research and development of nearly 40 years
Achievement.China's double-linear hybrid rice development is swift and violent, by the end of the end of the year 2010, has 427 double-line hybrid combinations and has passed through to save
Level or national new varieties authorization, wherein having 62 by the kind that national new varieties are authorized.2005 to 2011, Ministry of Agriculture's hat
In 83 kinds of name " super hybridization rice ", double-linear hybrid rice accounts for 17.1996~2014 years, China had more than 900 two line method miscellaneous
Rice varieties are handed over by authorization, adds up the public song of popularizing area 50,000,000,5,500,000 hectares or so of current year popularizing area accounts for complete
35% or so of state's hybrid rice sowing area, has become the main path of rice heterosis utilization, is ensureing grain peace
Important function has been played in complete.
One of the reason for limitation two-line hybrid rice popularization one is important is the breeding of photo-thermo-sensitive genetic male sterile line.China's light is temperature sensitive not
It is breeding main in the breeding of Hainan winter to educate, and because Hainan weather conditions are richly endowed by nature, agricultural planting industry and tourist industry are flourishing, make
It is nervous into agricultural land and rent is more and more expensive.Seed is bred in Hainan, is bred the seed demand for harvesting and is transported for long-distance back lake
The ground such as south carry out the production of hybrid seeds, transport and rent of soil high cost, cause male-sterile seed cost very high.Another method is to adopt
Take large reservoir and take bottom cold water and irrigated in temp-sensing sterile line fertility sensitive period, due to place selection require it is high, it is difficult to
Suitable place is found, while needing the special messenger to carry out irrigation management in tricky time, causes cost also very high, it is therefore necessary to
Carry out the research of new two-line sterile line propagation method.
During bilinear hybrid use of advantage, efficiently breeding two is that line with genic sterile is always that restriction double-linear hybrid rice is promoted
One of key factor.Lot of experiments analysis shows, it is very narrow that temperature sensitive type two-line sterile line can educate temperature range, most sterile lines
It is required that in fertility-sensitive period（10-25 days before beginning fringe）Daily mean temperature will meet this temperature less than 22 DEG C and higher than 19 DEG C
The place of the Climatic of condition is difficult to find.Currently used for production two is that line with genic sterile seed is typically all from cold water string irrigating
There is very big risk in breeding and the breeding of Hainan in Winter natural low temperature, both modess of reproduction.
The subject matter that cold water string irrigating breeding is present
Needed when cold water string irrigating is bred the cold water of 19 DEG C or so of freezer bottom from one side of field fill into, from another side discharge,
By the flowing of water at low temperature, make field water temperature be maintained at below sterile line critical temperature of sterility and physiology infertility lower limit temperature more than
Within the scope of, so to base and demanding strict technology, area of base is limited, builds that input cost is big, and production cost is too high；
Cold water string irrigating needs cold water uninterruptedly to irrigate 15~20 days, causes the waste of water resource, is conflicted with water with large area Rice Production,
Cold water resource cannot be guaranteed；19~22 DEG C of the water temperature requirement of cold-penetration water, temperature is too low to cause physiology infertility, even 19 DEG C
Cold water go here and there for a long time filling can also cause the solid bad of water inlet field plant, cold water flow to the ground of delivery port through certain distance
Just, temperature at this moment can exceed that the critical temperature of sterility of sterile line, and so as to cause part field setting percentage relatively low, cold-penetration can also
Make part field completely shaky or solid very poor because circulating water dead angle occurs；Cold-penetration breeding heading flowering period productive phase temperature
Degree is high, causes yield relatively low.
The problem that Hainan in Winter breeding is present
The educated temperature range of paddy rice dual-purpose line with genic sterile fertility-sensitive period is narrower, especially the low infertility of critical temperature of sterility
System, can most preferably educate temperature range for 20.0~21.5 DEG C of daily mean temperature, and paddy rice dual-purpose core is bred not under the natural conditions of Hainan
Educate and be, fertility-sensitive period runs into the probability very little of this temperature, abnormal low temperature widow's photograph can not ear or temperature is too high causes numerous
Plant and fall flat.Hainan winter is numerous bring another problem is that of poor quality, it is paddy rice to make before Hainan winter is numerous, and the problem of shattering paddy is serious
Influence breeding purity, another aspect Hainan winter, numerous often yield was relatively low, and in the case of low-producing, hybrid strain string powder and selfing are produced
Seed rate increase, male-sterile seed do not reach production kind requirement.
Sterile line propagation is carried out using the technology of the above, major defect and deficiency are：Soil, artificial and cost of transportation be high,
Efficiency is low, risk is big.This technology is simple and practical, and the production of hybrid seeds is combined with breeding, it is not necessary to increase extra input and complexity is grasped
Make, the root and stem of certain plants and flowering time are stayed as long as can get hold of and cut fringe, using the condition of later stage temperature step-down, so as to realize that two-line sterile line is numerous
Grow and be.
The content of the invention
In view of this, it is simple and practical it is an object of the invention to provide a kind of simple two-line sterile line propagation method, only need
Get hold of and cut fringe and stay the root and stem of certain plants and flowering time, using the condition of later stage temperature step-down, two-line sterile line line of breeding can be realized.
The technical scheme is that：A kind of simple two-line sterile line propagation method, comprises the following steps：
（1）Under the weather conditions of Changsha, selection two-line sterile line seed is sowed in April；
（2）After planting transplanted in May, expanded sterile line strain number by point root and stem of certain plants in 6-7 months, and by sterile line strain and recovered
It is combo, carrying out artificial supplementary pollination when July, heading was bloomed completes hybridization, and until by the end of August, left and right has harvested hybrid seed；
（3）When harvesting seed, tassel can be first sliced off, retain the standing grain root and stem of certain plants；
（4）To step（3）The seed of results is irrigated using alternation of wetting and drying, anti-to cure pest and disease damage, after under temperature after September part
Drop, sterile line can complete Fertility al-teration, and in the 9-10 months neat fringe, or so November can harvest the male-sterile seed of breeding.
Preferably, the step（3）The stubble height of the middle standing grain root and stem of certain plants is generally 35-40cm, and keeps down two buds here, keeps down three
Bud is beneficial to the raising effective fringe of ratooning rice.
Preferably, the step（4）The setting percentage of middle male-sterile seed is up to more than 50%.
Preferably, the step（4）The setting percentage of middle male-sterile seed reaches 50%-80%.
Compared with prior art, the present invention has the advantages that：A kind of simple two-line sterile line that the present invention is provided
Propagation method, the production of hybrid seeds is combined with breeding, simple and practical, reduces production cost, it is only necessary to is got hold of to cut fringe and stay and the root and stem of certain plants and is bloomed
Time, using the condition of later stage temperature step-down, two-line sterile line line of breeding can be realized；The present invention can realize that low cost is numerous
Grow, and two-line sterile line adding generation can be realized, accelerate sterile line stabilization, not by local limit propagation, setting percentage can be with
50%-80% is reached, later stage weather fine day is short of rain, and the seed of breeding is full, and seed quality is relatively good, the pest and disease damage such as few smut
Injury.
Brief description of the drawings
Fig. 1 is flow chart of the invention.
Specific embodiment
Below in conjunction with the accompanying drawings and specific embodiment, the present invention will be further described.
As shown in figure 1, a kind of simple two-line sterile line propagation method, comprises the following steps：
（1）Under the weather conditions of Changsha, selection two-line sterile line seed is sowed in April.
（2）After planting transplanted in May, expanded sterile line strain number by point root and stem of certain plants in 6-7 months, and by sterile line strain with
Restorer combo, the artificial supplementary pollination when July, heading was bloomed, until by the end of August, left and right has harvested hybrid seed, in high temperature bar
Hybrid rice seed production is completed under part.
（3）When harvesting seed, tassel can be first sliced off, retain the standing grain root and stem of certain plants, the stubble height of the standing grain root and stem of certain plants is generally 35-40cm,
Typically keep down two buds here, keep down three buds, being conducive to improving the effective fringe of ratooning rice.
（4）To step（3）The seed of results is irrigated using alternation of wetting and drying, anti-to cure pest and disease damage, after temperature after September part
Decline, sterile line can complete Fertility al-teration, in the 9-10 months neat fringe, left and right can harvest breeding not by the end of October or at the beginning of 11 months
It is seed to educate, and up to more than 50%, the setting percentage of good male-sterile seed can reach 50%- to the self-fruitful rate of male-sterile seed
80%。
A kind of simple two-line sterile line propagation method that the present invention is provided, in the case of cost is not increased, and by the production of hybrid seeds
Utilization is combined with breeding, it is simple and practical, it is only necessary to get hold of and cut fringe and stay the root and stem of certain plants and flowering time, using the bar of later stage temperature step-down
Part, can realize that two-line sterile line is bred；The present invention can realize inexpensive breeding, and can realize that two-line sterile line adds generation
Breeding, accelerates sterile line stabilization, and not by local limit propagation, setting percentage can reach 50%-80%, and later stage weather fine day is short of rain,
The seed of breeding is full, and color is naturally yellow and vivid, and seed quality is relatively good, the injury of the pest and disease damage such as few smut.Sterile line
Reproductive output can reach yield 2-3 tons/hectare, and seed purity can reach more than 99%, and more than 98%, germination percentage reaches cleanliness
More than 85%.
The above embodiments are only the preferred embodiments of the present invention, it is impossible to interest field of the invention is limited with this, because
This, modification, equivalent variations, improvement for being made according to scope of the present invention patent etc. still belong to the scope that the present invention is covered.
Claims (4)
1. a kind of simple two-line sterile line propagation method, it is characterised in that comprise the following steps：
Under the weather conditions of Changsha, selection two-line sterile line seed is sowed in April；
After planting transplanted in May, expanded sterile line strain number by point root and stem of certain plants in 6-7 months, and sterile line is matched somebody with somebody with restorer
Group, artificial supplementary pollination is carried out when July, heading was bloomed and completes to hybridize, and hybrid seed has been harvested to left and right by the end of August；
When harvesting seed, tassel can be first sliced off, retain the standing grain root and stem of certain plants；
To step（3）The seed of results is irrigated using alternation of wetting and drying, anti-to cure pest and disease damage, after mercury dropped after September part, no
It is that can complete from infertility to fertile Fertility al-teration to educate, and in the 9-10 months neat fringe, or so November can harvest the infertility of breeding
It is seed.
2. a kind of simple two-line sterile line propagation method according to claim 1, it is characterised in that the step（3）In
The stubble height of the standing grain root and stem of certain plants is generally 35-40cm, and keeps down two buds here, keeps down three buds and be beneficial to the raising effective fringe of ratooning rice.
3. a kind of simple two-line sterile line propagation method according to claim 1, it is characterised in that the step（4）In
The setting percentage of male-sterile seed is up to more than 50%.
4. a kind of simple two-line sterile line propagation method according to claim 3, it is characterised in that the step（4）In
The setting percentage of male-sterile seed reaches 50%-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710172011.1A CN106857132A (en) | 2017-03-22 | 2017-03-22 | A kind of simple two-line sterile line propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710172011.1A CN106857132A (en) | 2017-03-22 | 2017-03-22 | A kind of simple two-line sterile line propagation method |
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| CN106857132A true CN106857132A (en) | 2017-06-20 |
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| CN201710172011.1A Pending CN106857132A (en) | 2017-03-22 | 2017-03-22 | A kind of simple two-line sterile line propagation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258454A (en) * | 2018-10-23 | 2019-01-25 | 安徽绿亿种业有限公司 | A kind of breeding method of the high-yield hybrid rice of anti-rice leaf smut |
| CN110100671A (en) * | 2019-05-28 | 2019-08-09 | 雅安雨禾农业科技有限责任公司 | A kind of rice regeneration propagation method |
| CN111837939A (en) * | 2020-07-30 | 2020-10-30 | 吴烽 | Method for fixing rice heterosis |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699987A (en) * | 2009-11-27 | 2010-05-05 | 天津天隆农业科技有限公司 | Method for accelerating seed-selecting process of japonica type two-line sterile line |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN103444510A (en) * | 2013-08-14 | 2013-12-18 | 江苏丘陵地区镇江农业科学研究所 | Safe and efficient method for propagating breeder seeds of two-line sterile line and special isolation hood thereof |
| CN104770291A (en) * | 2015-04-20 | 2015-07-15 | 中国水稻研究所 | Selective breeding method for two-line paddy sterility line with low sterile critical temperature |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
| CN106386312A (en) * | 2016-09-18 | 2017-02-15 | 四川农业大学 | Method for propagation and seed production of two-line sterile lines of rice |
-
2017
- 2017-03-22 CN CN201710172011.1A patent/CN106857132A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699987A (en) * | 2009-11-27 | 2010-05-05 | 天津天隆农业科技有限公司 | Method for accelerating seed-selecting process of japonica type two-line sterile line |
| CN101743872A (en) * | 2009-12-11 | 2010-06-23 | 湖北省种子集团有限公司 | Method for breeding two-line sterile line of rice |
| CN103444510A (en) * | 2013-08-14 | 2013-12-18 | 江苏丘陵地区镇江农业科学研究所 | Safe and efficient method for propagating breeder seeds of two-line sterile line and special isolation hood thereof |
| CN104770291A (en) * | 2015-04-20 | 2015-07-15 | 中国水稻研究所 | Selective breeding method for two-line paddy sterility line with low sterile critical temperature |
| CN105638347A (en) * | 2016-01-20 | 2016-06-08 | 安徽理想种业有限公司 | Rice two-line sterility line propagation method |
| CN106386312A (en) * | 2016-09-18 | 2017-02-15 | 四川农业大学 | Method for propagation and seed production of two-line sterile lines of rice |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258454A (en) * | 2018-10-23 | 2019-01-25 | 安徽绿亿种业有限公司 | A kind of breeding method of the high-yield hybrid rice of anti-rice leaf smut |
| CN109258454B (en) * | 2018-10-23 | 2021-10-19 | 安徽绿亿种业有限公司 | Breeding method of high-yield hybrid rice capable of resisting rice leaf smut |
| CN110100671A (en) * | 2019-05-28 | 2019-08-09 | 雅安雨禾农业科技有限责任公司 | A kind of rice regeneration propagation method |
| CN110100671B (en) * | 2019-05-28 | 2021-07-27 | 雅安雨禾农业科技有限责任公司 | Rice regeneration propagation method |
| CN111837939A (en) * | 2020-07-30 | 2020-10-30 | 吴烽 | Method for fixing rice heterosis |
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