CN101699987A - Method for accelerating seed-selecting process of japonica type two-line sterile line - Google Patents
Method for accelerating seed-selecting process of japonica type two-line sterile line Download PDFInfo
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- CN101699987A CN101699987A CN200910228818A CN200910228818A CN101699987A CN 101699987 A CN101699987 A CN 101699987A CN 200910228818 A CN200910228818 A CN 200910228818A CN 200910228818 A CN200910228818 A CN 200910228818A CN 101699987 A CN101699987 A CN 101699987A
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Abstract
The invention relates to a method for accelerating the seed-selecting process of japonica type two-line sterile line. The invention provides an 'identifying and breeding synchronization' method, thereby not only being capable of realizing a target for breading the seed in a primary method, but also being capable of greatly accelerating the process, namely, the effect of 3 years with the method can reach that of 5-6 yeas with the prior art. The method identifies the fertility of pollen at the north China with a microscope, can harvest autogamous seed at the same grow season, and further adds one generation at Hainan, thereby realizing the seed-selecting process with one year two generations, and being capable of breeding the new japonica type two-line sterile line within 3 years.
Description
The invention belongs to the technique for breeding of hybridized rice field, be specifically related to a kind of method of accelerating seed-selecting process of japonica type two-line sterile line.
Background technology
At present, the selection of the round-grained rice type two-line sterile line that is widely used is " mirror south, north is numerous ", promptly carries out fertility in the north and identifies, carries out numerous kind of operation in south.Concrete grammar is natural conditions of utilizing the north, two-line sterile line is carried out fertility observe, with definite sterile individual plant, uproot and take Hainan to, and utilize the cryogenic conditions (being generally for 2～March) in area, Hainan, its fertility is changed naturally, thereby obtained two-line sterile line new lines seed.Utilize this method, can make every year unstabilized strain increase a generation, obtain stable novel two-line sterile line, need the time in 6～8 years at least.
Summary of the invention
In order to overcome the slow deficiency of prior art breeding process, the invention provides the method for a kind of " it is numerous synchronous to reflect ", not only can realize the breeding objective of former method, and can accelerate its process significantly, utilize the time about 3 years, reach the effect that former method need just can reach in 5～6 years.
A kind of method of accelerating seed-selecting process of japonica type two-line sterile line that the present invention relates to may further comprise the steps: utilize the method for pollen staining, microexamination, the target individual plant is made fertility judge at paddy rice loose powder initial stage of blooming; Can educate if tested individual plant is a pollen, then be eliminated; If tested individual plant is a pollen sterility, stay the selfing of 1～2 tassel bagging and further identify fertility, remaining tassel slices off; Impose a small amount of nitrogenous fertilizer after cutting fringe, new tillering takes place in short its once more; The tillering tassel that the back takes place is caught up with local cryogenic conditions in meiosis stage, makes it realize the fertility conversion automatically, and self-fertility is when growth can be received seed in season;
Definite method of cutting the fringe time is as follows: (1) determines the fertility conversion desired temperature of the two-line sterile line of desire seed selection; (2) according to the on-site climatic variation rule data over the years of breeding, find out the time that desired temperature appears in the locality; (3) before appearring in the locality, cut the time of desired temperature fringe in 13-20 days.For example, the fertility of above-mentioned two-line sterile line conversion desired temperature is 22-23 ℃.
Concrete, the technical solution adopted in the present invention is: utilize the method for pollen staining, microexamination, the target individual plant is made fertility judge at paddy rice loose powder initial stage of blooming.The principle of institute's foundation is that starch is met the phenomenon that iodine becomes indigo plant.Can educate if tested individual plant is a pollen, then be eliminated; If tested individual plant is a pollen sterility, then slice off existing most tassels, stay the selfing of 1～2 tassel bagging, purpose is to allow its continued growth, treats further to confirm autumn its solid situation under the bagging situation, further to determine its fertility.Impose a small amount of nitrogenous fertilizer after cutting fringe, utilize the shooting property of paddy rice, new tillering takes place in short its once more.The tillering tassel that the back takes place is caught up with local cryogenic conditions in meiosis stage, makes it realize the fertility conversion automatically, and self-fertility is when growth just can be received seed in season.
The key of this technical scheme is to select the suitable fringe period of cutting, if it is too early to cut fringe, then the tillering tassel that takes place of back is met higher temperature meiosis stage, and the target individual plant is still sterile, can't obtain seed; If it is late excessively to cut fringe, then the tillering tassel late growth stage temperature that takes place of back is lower, and tillering tassel can not normal mature, also can't obtain seed.
Definite method of cutting the fringe time is as follows: (1) according to present breeding level and breeding objective, the fertility conversion desired temperature of the two-line sterile line of plan seed selection should be 22-23 ℃; (2) according to on-site climatic variation rule data over the years, find out the approximate time that desired temperature appears in the locality, for example northern area approximately be early August to late September, Efficiency in Buildings in Tianjin Area is early September; (3) according to the regeneration capacity of kind, prediction enters the needed time of meiosis stage from cutting fringe to regenerated tiller children fringe, generally needs 13-20 days; (4) determine to cut optimum period of fringe according to above data, guarantee that regenerated tiller children fringe can be in the environment of desired temperature in meiosis stage, realizes changing naturally of fertility.
By technique scheme, the sterile strain one of target obtains seed surely.Reason is: (1) rice tillering fringe be a continuous process again, and the developmental process of each small ear different parts grain husk flower also is continuous, more than two factors determined the course of blooming that tillering tassel takes place again will continue a very long time (in general will continue more than one month), even it is solid that early stage regeneration tillering tassel can not be met low temperature, the regeneration tillering tassel of Chou Chuing also can self-fertility so after a while; (2) temperature in northern autumn is to present the trend that reduces gradually, can drop in the two weeks after paddy rice blooms below the two-line sterile line fertility inversion temperature, and the tillering tassel that the later stage extracts out necessarily can carry out the fertility conversion; (3) less in the grain weight of northern this season needs, generally have about the 80-100 grain to get final product, be easy to satisfy the demands; (4) Quan Guogedi weather conditions differ greatly, and the time of cutting fringe is inevitable different, can calculate according to above principle.If can not accurately predict in 1 year and cut the fringe phase, also can suitably postpone and cutting the fringe time, guarantee that tillering tassel can be under the fertility inversion temperature condition at subtrahend tillering stage.Facts have proved that the best period that Efficiency in Buildings in Tianjin Area is cut fringe is mid-August, according to above principle operation, most strains can receive that selfed seed more than 300, enough satisfies the breeding work needs at this season.
Through the several generations successive selection, treat that the target strain is basicly stable after, can utilize now mature common technology that its coordinate force and fertility inversion temperature are detected, be used as new two-line sterile line to determine optimum strain.
Beneficial effect
The invention has the beneficial effects as follows, can finish " it is numerous to reflect " two tasks, change the breeding method of original " mirror south, north is numerous " up hill and dale, the breeding progress is doubled in a northern season.For the reestablish diplomatic relations material of breeding of needs, on the basis that breeding process doubles, can save the time in 1 year again.
Embodiment
The present invention is further described below in conjunction with embodiment.
(1) according to present breeding level and breeding objective, the fertility conversion target temperature that we will plan the new round-grained rice type two-line sterile line of seed selection is positioned at 22.3 ℃;
On August 19th, (2) 2006, with training short 64 is maternal, and Tianjin former 45 is a male parent, 25 of preparing hybrid kinds, take Hainan to winter in the same year and add the Dai Bingshou kind, about 2000 strains of F2 colony are planted in experiment station, Tianjin Tian Long Agricultural Science And Technology Co., Ltd Ninghe on April 15th, 2007;
(3) according to the data of local meteorological department, the mean temperature of Efficiency in Buildings in Tianjin Area early September approximately is about 20 ℃, satisfies the breeding requirement; We have strain now, enter the needed time of meiosis stage to regenerated tiller children fringe and are about 15 days from cutting fringe; According to above data, the time that we will cut fringe is decided to be mid-August; If cut fringe according to schedule, tillering tassel time of origin again estimates that be early October the first frost season of Tianjin in early September, and tillering tassel has the time enough maturation;
On August 5th～16, (4) 2007, with microscope pollen fertility is carried out microscopy, in conjunction with economical characters such as plant type, resistances, pollen is accredited as 174 sterile individual plants cuts the fringe processing, every strain keeps the selfing of 1-2 fringe bagging, to identify this strain fertility; By the end of on September 15th, 2007, there have been 162 individual plants to grow tillering tassel once more; By the end of on October 10th, 2007, the individual plant that grows tillering tassel was all received this season seed, and grain weight is all more than 100.It is solid that the selfing that every strain keeps identifies that fringe does not have, and proves that 162 strains are fully sterile.
(5) the regeneration fringe seed of Huo Deing is all mailed the operation that above-mentioned steps (3)-(4) are repeated in Hainan on November 3rd, 2007, carries out follow-on seed selection work, has realized the breeding process of in the north " it is numerous synchronous to reflect ";
(4) 2008 years, repeat the operation of above-mentioned steps (3)-(5), use the same method and carry out quick breeding, determine that 37 strain systems carry out primary part observation;
Repeat the operation of above-mentioned steps (3)-(4) (5) 2009 spring and summers, and it is stable to add generation; Winter in 2009,5 strain systems to emphasis carried out the evaluation of each side such as fertility in Hainan, and No. 6 strain system has outstanding performance, and finally named to be grand 6S.
Claims (2)
1. method of accelerating seed-selecting process of japonica type two-line sterile line is characterized in that may further comprise the steps:
Utilize the method for pollen staining, microexamination, the target individual plant is made fertility judge at paddy rice loose powder initial stage of blooming; Can educate if tested individual plant is a pollen, then be eliminated; If tested individual plant is a pollen sterility, stay the selfing of 1～2 tassel bagging and further identify fertility, remaining tassel slices off; Impose a small amount of nitrogenous fertilizer after cutting fringe, new tillering takes place in short its once more; The tillering tassel that the back takes place is caught up with local cryogenic conditions in meiosis stage, makes it realize the fertility conversion automatically, and self-fertility is when growth can be received seed in season;
Definite method of cutting the fringe time is as follows: (1) determines the fertility conversion desired temperature of the two-line sterile line of desire seed selection; (2) according to the on-site climatic variation rule data over the years of breeding, find out the time that desired temperature appears in the locality; (3) before appearring in the locality, cut the time of desired temperature fringe in 13-20 days.
2. according to the method for claim 1, it is characterized in that the fertility conversion desired temperature of described two-line sterile line is 22-23 ℃.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155852A (en) * | 2011-12-13 | 2013-06-19 | 天津天隆种业科技有限公司 | Technical method for cultivating optical thermosensitive genetic male sterile line with low fertility transformation starting point temperature |
| CN105393863A (en) * | 2015-12-02 | 2016-03-16 | 温州科技职业学院 | Paddy rice photo-thermal-sensitive sterile line generation-adding method |
| CN105557511A (en) * | 2016-01-20 | 2016-05-11 | 安徽理想种业有限公司 | Method for accelerating rice breeding progress |
| CN106857132A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of simple two-line sterile line propagation method |
-
2009
- 2009-11-27 CN CN200910228818A patent/CN101699987A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155852A (en) * | 2011-12-13 | 2013-06-19 | 天津天隆种业科技有限公司 | Technical method for cultivating optical thermosensitive genetic male sterile line with low fertility transformation starting point temperature |
| CN105393863A (en) * | 2015-12-02 | 2016-03-16 | 温州科技职业学院 | Paddy rice photo-thermal-sensitive sterile line generation-adding method |
| CN105557511A (en) * | 2016-01-20 | 2016-05-11 | 安徽理想种业有限公司 | Method for accelerating rice breeding progress |
| CN106857132A (en) * | 2017-03-22 | 2017-06-20 | 湖南杂交水稻研究中心 | A kind of simple two-line sterile line propagation method |
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