CN102771384A - Method for seed production of two-line hybrid rice - Google Patents
Method for seed production of two-line hybrid rice Download PDFInfo
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- CN102771384A CN102771384A CN2012102990149A CN201210299014A CN102771384A CN 102771384 A CN102771384 A CN 102771384A CN 2012102990149 A CN2012102990149 A CN 2012102990149A CN 201210299014 A CN201210299014 A CN 201210299014A CN 102771384 A CN102771384 A CN 102771384A
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Abstract
The invention discloses a method for seed production of two-line hybrid rice. The method comprises the following steps of: hybridizing a restoring line carrying herbicide-resistant genes and a sterile line which does not contain the herbicide-resistant genes to produce hybrid rice seeds; and planting the harvested hybrid rice seeds with two genotypes in the next year, planting the hybrid rice seeds, and spraying herbicides once or twice before seedling transplanting, wherein the hybrid rice seeds surviving from the herbicides are the real hybrid rice seeds. According to the method, the breeding of the sterile line of the hybrid rice is simplified, the purity of hybrid rice seedlings in a rice field can be greatly improved, the impurity removal operation in the field at the later stage of the seedling transplanting is avoided, lots of manpower and material resources are saved, the yield is improved, and the labor efficiency is greatly improved.
Description
Technical field
The present invention relates to the producing method for seed of a kind of hybrid rice; Clear specifically on the one hand through low fertility male sterile line self-fertility breeding male sterile lines, in the breeding of hybrid rice process, kill the method that the self-fruitful false crossbreed of male sterile line guarantees true purity of hybrid on the other hand through weed killer herbicide.
Background technology
Produce the hybrid rice that goes up the large tracts of land popularizing planting at present and mainly comprise double-linear hybrid rice and series of three-series hybrid rice, it is male sterile line that corresponding male sterile line is divided into warm photosensitive two-line sterile line and three.The temperature photosensitive sterile line has the performance male sterile down of long-day, high temperature, and short-day, low temperature down can normally solid characteristics, are that male sterile line is compared with three, and warm photosensitive sterile line breeds that operation is simple; But also brought a problem simultaneously; The fertility of temperature photosensitive sterile line is controlled by weather conditions fully; The uncertainty of weather conditions brings heavy losses for the breeding of photo-thermo-sensitive genetic male sterile line, selects a kind of two-line sterile line that does not receive weather influence the development of hybrid rice is had huge impetus.
In the breeding of hybrid rice process; What production of hybrid seeds unit was concerned about most is the purity of hybrid rice seeds; Can't use on producing due to hybrid rice seeds purity is not enough and bring enormous economic loss, provide a kind of producing method for seed that can guarantee hybrid rice seeds purity that the development of hybrid rice is had major contribution to vast production of hybrid seeds unit.
Summary of the invention
In order to overcome the deficiency of prior art; The object of the present invention is to provide a kind of producing method for seed of double-linear hybrid rice; Being intended to solve the photosensitive two-line sterile line of present temperature, three is that male sterile line production receives weather influence, loaded down with trivial details, the high problem of bringing of reproductive-cost of production process, has proposed a kind of novel male sterile line and has used pattern; Simultaneously, utilize the characteristic of antiweed dominant character, solved that breeding of hybrid rice purity is not high, the loaded down with trivial details a series of problems brought of impurity elimination.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopted is:
A kind of producing method for seed of double-linear hybrid rice is characterized in that, this method may further comprise the steps:
(1) a kind of economical character of seed selection good, can self-fruitful male sterile line, do not contained the male sterile line of anti-herbicide gene through the male sterile line self propagated;
(2) anti-herbicide gene is imported the recovery system of recovering to obtain in the system carrying anti-herbicide gene, making recovery is the characteristic that paddy rice possesses antiweed;
(3) hybridize with the male sterile line that does not contain anti-herbicide gene that obtains in recovery system of carrying anti-herbicide gene that obtains in the step (2) and the step (1); Obtain two kinds of genotypic rice paddy seeds during results: a kind of is the true crossbreed that antiweed recovers system and male sterile line; Carry anti-herbicide gene, possess the characteristic of antiweed; A kind of in addition is the self-fruitful offspring of male sterile line, i.e. pseudostationary does not possess the characteristic of antiweed;
(4) two kinds of genotypic rice paddy seeds of results in the next year plantation step (3) obtain true crossbreed after herbicide treatment.
Compared with prior art, beneficial effect of the present invention is:
The ⒈ sterile line propagation is simple to operate, low production cost.With three is that the breeding of male sterile line, warm photosensitive two-line sterile line is compared; Need consider that neither three is the problem of male sterile line in the breeding, maintenance line flower synchronization, need not consider that also temperature photosensitive two is that temperature in the breeding, sunshine are to the solid influence of the photosensitive two-line sterile line of temperature.Technical scheme of the present invention is that reproductive process is very easy, can obtain highly purified male-sterile seed, can reduce labor intensity again, reduces production costs through male sterile line self-fertility breeding male sterile lines seed.
2. need not rogueing, reduce labor intensity.Male sterile line carries anti-herbicide gene with the true hybrid that the recovery system hybridization of carrying anti-herbicide gene produces, and has the characteristic of antiweed; And the pseudostationary that the male sterile line selfing produces does not contain anti-herbicide gene, does not possess the characteristic of antiweed.Utilize weed killer herbicide to kill pseudostationary and true hybrid is not influenced this characteristic; Can overcome the problem that to use because of in the production of hybrid rice seeds purity due to not enough; Reduce the time of rogueing, reduce labour intensity, improve the labor productivity of breeding of hybrid rice.
3. weed killer herbicide impurity elimination, hybrid rice rice shoot purity is high.The plantation hybrid rice sprays weed killer herbicide 1-2 time before shoot transplanting equipment, do not receive the true crossbreed that is that weed killer herbicide influences.Can increase substantially the purity of hybrid rice rice shoot through this method, exempt the impurity elimination work in shoot transplanting equipment later stage field, both save great amount of manpower, improve output again, improve efficiency greatly.
Description of drawings
Fig. 1 is the flow chart of male sterile line self propagated among the present invention;
Fig. 2 is the producing method for seed realization flow figure of double-linear hybrid rice among the present invention.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further specified below in conjunction with accompanying drawing and embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in the qualification invention.
Male sterile line of the present invention, recovery system source:
Male sterile line: town rice 286A is the Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute through the combination town rice 88A that reestablishes diplomatic relations //H6/ in a ripening rate obtaining of round-grained rice 511 seed selections at the novel male sterile line of 10%-15%.
Recover system: pressing down extensively 214, is that the Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute passes through that round-grained rice recovers system in good and 218 antiweeds obtaining with the seed selection of antiweed (resistance glyphosate) incross.
This method may further comprise the steps:
1. the breeding of male sterile line, because town rice 286A has self-fruitful ability, can be through the method breeding male sterile lines town rice 286A of conventional japonica rice breeding.
2. with town of carrying anti-herbicide gene extensive 214 and the town rice 286A hybridization that does not contain anti-herbicide gene, obtain two kinds of genotypic rice paddy seeds during results: a kind of is the crossbreed F of antiweed kind town extensive 214 and male sterile line town rice 286A
1(carry anti-herbicide gene, possess the characteristic of antiweed), a kind of in addition is the self-fruitful offspring of male sterile line town rice 286A (be pseudostationary, do not possess the characteristic of antiweed).
3. next year is planted hybrid rice seeds; With 10% gyphosate solution dilution 40-60 doubly; Before shoot transplanting equipment, evenly spray 1-2 time; Kill the self-fruitful pseudostationary of the male sterile line that does not contain anti-herbicide gene town rice 286A, the survival antiweed be true crossbreed (promptly pressing down rice 286A/ town extensive 214).Carry out weeding with 10% gyphosate solution and handle, will improve the purity of hybrid rice rice shoot on the one hand as far as possible, can not cause poisoning to the hybrid rice rice shoot on the other hand.
Claims (7)
1. the producing method for seed of a double-linear hybrid rice is characterized in that, this method may further comprise the steps:
(1) a kind of economical character of seed selection good, can self-fruitful male sterile line, do not contained the male sterile line of anti-herbicide gene through the male sterile line self propagated;
(2) anti-herbicide gene is imported the recovery system of recovering to obtain in the system carrying anti-herbicide gene, making recovery is the characteristic that paddy rice possesses antiweed;
(3) hybridize with the male sterile line that does not contain anti-herbicide gene that obtains in recovery system of carrying anti-herbicide gene that obtains in the step (2) and the step (1), obtain two kinds of genotypic rice paddy seeds during results: a kind of is the true crossbreed that antiweed recovers system and male sterile line; A kind of in addition is the self-fruitful offspring of male sterile line, i.e. pseudostationary;
(4) two kinds of genotypic rice paddy seeds of results in the next year plantation step (3) obtain true crossbreed after herbicide treatment.
2. the producing method for seed of double-linear hybrid rice according to claim 1 is characterized in that, used male sterile line is the novel male sterile line of a kind of self-fruitful rate at 10%-15% in step (1), and can pass through male sterile line self-fertility breeding male sterile lines.
3. the producing method for seed of double-linear hybrid rice according to claim 1 and 2 is characterized in that, said male sterile line is town rice 286A.
4. the producing method for seed of double-linear hybrid rice according to claim 1 is characterized in that, is town extensive 214 in the system of the recovery described in the step (2).
5. the producing method for seed of double-linear hybrid rice according to claim 1 is characterized in that, described true crossbreed is the rice paddy seed that carries anti-herbicide gene and possess the antiweed characteristic, and said pseudostationary is not contain anti-herbicide gene.
6. the producing method for seed of double-linear hybrid rice according to claim 1; It is characterized in that; In step (4), said herbicide treatment method is: after 40-60 times of 10% gyphosate solution dilution, before shoot transplanting equipment, evenly be sprayed on the rice shoot; To improve the purity of hybrid rice rice shoot on the one hand as far as possible, can not cause poisoning to the hybrid rice rice shoot on the other hand.
7. the producing method for seed of double-linear hybrid rice according to claim 6 is characterized in that, sprays 1-2 time.
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| CN2012102990149A CN102771384A (en) | 2012-08-22 | 2012-08-22 | Method for seed production of two-line hybrid rice |
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| CN2012102990149A CN102771384A (en) | 2012-08-22 | 2012-08-22 | Method for seed production of two-line hybrid rice |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934606A (en) * | 2012-12-04 | 2013-02-20 | 江苏里下河地区农业科学研究所 | Hybrid rice mechanical seed production technology |
| CN104206268A (en) * | 2014-09-09 | 2014-12-17 | 扬州大学 | Novel method for sensitively cultivating efficient rice intelligent sterile line by using herbicides |
| CN104429935A (en) * | 2014-12-12 | 2015-03-25 | 扬州大学 | New method for increasing seed reproduction yield of rice intelligent sterile line |
| CN105104166A (en) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | Method used for breeding imidazolinone herbicide-resistant japonica rice |
| CN106386312A (en) * | 2016-09-18 | 2017-02-15 | 四川农业大学 | Method for propagation and seed production of two-line sterile lines of rice |
| CN109169259A (en) * | 2018-09-27 | 2019-01-11 | 上海市农业科学院 | A kind of selection of herbicide-resistant two-line sterile line of rice |
| CN110291986A (en) * | 2017-09-28 | 2019-10-01 | 彭基明 | Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
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| CN1047009A (en) * | 1989-05-09 | 1990-11-21 | 王保明 | The new method of producing hybrid wheat seed and sterile line propagation thereof |
| CN1114524A (en) * | 1995-04-19 | 1996-01-10 | 邓祚荣 | Method for breeding rice male sterile line by using cutting sensitive sterile line |
| CN1799343A (en) * | 2005-12-28 | 2006-07-12 | 吉林省农业科学院 | Method for production of novel anti-glyphosate germplasm of kentucky bluegrass |
| WO2009020656A1 (en) * | 2007-08-07 | 2009-02-12 | Washington State University | Glyphosate-tolerant wheat genotypes |
| CN101491210A (en) * | 2009-02-25 | 2009-07-29 | 西南大学 | Building method of plant genetic engineer male sterility line |
| CN101960987A (en) * | 2010-10-21 | 2011-02-02 | 湖北省农业科学院粮食作物研究所 | Seed production method for hybrid rice |
| CN102308748A (en) * | 2010-05-20 | 2012-01-11 | 北京未名凯拓作物设计中心有限公司 | Optimized method for screening transgenic crops by coating herbicide |
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2012
- 2012-08-22 CN CN2012102990149A patent/CN102771384A/en active Pending
Patent Citations (7)
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| CN1047009A (en) * | 1989-05-09 | 1990-11-21 | 王保明 | The new method of producing hybrid wheat seed and sterile line propagation thereof |
| CN1114524A (en) * | 1995-04-19 | 1996-01-10 | 邓祚荣 | Method for breeding rice male sterile line by using cutting sensitive sterile line |
| CN1799343A (en) * | 2005-12-28 | 2006-07-12 | 吉林省农业科学院 | Method for production of novel anti-glyphosate germplasm of kentucky bluegrass |
| WO2009020656A1 (en) * | 2007-08-07 | 2009-02-12 | Washington State University | Glyphosate-tolerant wheat genotypes |
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| CN102308748A (en) * | 2010-05-20 | 2012-01-11 | 北京未名凯拓作物设计中心有限公司 | Optimized method for screening transgenic crops by coating herbicide |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934606A (en) * | 2012-12-04 | 2013-02-20 | 江苏里下河地区农业科学研究所 | Hybrid rice mechanical seed production technology |
| CN104206268A (en) * | 2014-09-09 | 2014-12-17 | 扬州大学 | Novel method for sensitively cultivating efficient rice intelligent sterile line by using herbicides |
| CN104429935A (en) * | 2014-12-12 | 2015-03-25 | 扬州大学 | New method for increasing seed reproduction yield of rice intelligent sterile line |
| CN105104166A (en) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | Method used for breeding imidazolinone herbicide-resistant japonica rice |
| CN105104166B (en) * | 2015-07-23 | 2021-05-07 | 上海市农业科学院 | Method for breeding imidazolinone herbicide-resistant japonica rice |
| CN106386312A (en) * | 2016-09-18 | 2017-02-15 | 四川农业大学 | Method for propagation and seed production of two-line sterile lines of rice |
| CN106386312B (en) * | 2016-09-18 | 2020-01-03 | 四川农业大学 | Method for breeding seeds of rice by two-line sterile line |
| CN110291986A (en) * | 2017-09-28 | 2019-10-01 | 彭基明 | Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
| CN109169259A (en) * | 2018-09-27 | 2019-01-11 | 上海市农业科学院 | A kind of selection of herbicide-resistant two-line sterile line of rice |
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Application publication date: 20121114 |