CN110291986A - Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof - Google Patents
Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof Download PDFInfo
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- CN110291986A CN110291986A CN201910700270.6A CN201910700270A CN110291986A CN 110291986 A CN110291986 A CN 110291986A CN 201910700270 A CN201910700270 A CN 201910700270A CN 110291986 A CN110291986 A CN 110291986A
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- herbicide
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- paddy seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The present invention provides a kind of rice paddy seed products containing anti-herbicide gene of field planting, wherein the product includes the herbicide that rice paddy seed containing anti-herbicide gene and the rice paddy seed containing anti-herbicide gene can be resisted.The present invention also provides the preparation methods of the said goods, wherein method includes the following steps: (1) hybridizes restorer or male parent with the rice breed containing anti-herbicide gene；(2) the rice paddy seed selfing obtained step (1) is one or many, either with the restorer perhaps male parent backcrossing it is one or many or with other mixing breeds it is primary or repeatedly；And the strain containing anti-herbicide gene that (3) obtain step (2) hybridizes with three systems or two-line sterile line, obtains the rice paddy seed containing anti-herbicide gene；(4) rice paddy seed containing anti-herbicide gene obtained with the herbicide treatment step (3).
Description
The present invention be submit on June 15th, 2018, application No. is 201810617736.1, it is entitled " a kind of big
The rice paddy seed product containing anti-herbicide gene and preparation method thereof of field plantation " divisional application.
Technical field
The present invention relates to rice paddy seed products containing anti-herbicide gene of a kind of field planting and preparation method thereof.
Background technique
Rice is always China's the first grande culture object.In recent years, hybrid rice is due to the features such as yield is high, adaptable kind
Area is planted constantly to expand.According to statistics, the cultivated area of hybrid rice accounts for 50% or more of the national rice gross area, hybrid rice
Plantation yield accounts for 60% or more of national rice total output.Therefore, hybrid rice is that China's grain-production is made that tremendous contribution,
Solves the crisis in food in China.
During the rice production of hybrid seeds, guarantee that hybrid rice seeds purity is to guarantee the important measures of hybrid rice yield.According to
According to GB4404.1-2008 " cereal crops corresponding seed quality criteria-Cereal ", it is necessary to assure hybrid rice seeds purity is not less than
96%, once because purity hybrid rice seeds mass selling not up to standard gives peasant household to carry out field planting, it is possible to cause big
The a large amount of underproduction in field bring huge loss to the even local agricultural production of plantation peasant household.Meanwhile if purity hybridization water not up to standard
Rice is unsalable at seeding farmer or enterprise, and huge loss will be also brought to them and influences its enthusiasm for production.
In order to improve purity, for series of three-series hybrid rice, it is necessary to be strictly isolated to seed farm, prevent other rice product
Kind pollen string is handed over.For example, the measure being strictly isolated includes following several.
(1) it is isolated using space length
According to the regulation of GB/T17314-2011 " indica hybrid rice " three systems " seed production regulations for technical operation ", infertility
System preferably uses nature to be isolated with different kind, space isolation distance 700m or more.In special circumstances, such as massif, the back of hilly country
Wind zone isolation area can suitably be reduced, but cannot be below 50m.The big Plain of wind-force cannot be less than predetermined distance 200m or
500m。
(2) it is isolated using the time at florescence
According to relevant regulations, the florescence in breeding of hybrid rice field and the florescence of other kind rice should be staggered, beginning ear period
It is staggered 25 days or more.
(3) it is isolated using vertical barrier
According to relevant regulations, the barrier for isolation hybridization rice seed farm includes that hillside, the woods, house and high bar are made
Object, barrier height should be higher than that 2 meters.
(4) it is isolated using other crops
According to relevant regulations, can be planted within the scope of 50 meters of breeding of hybrid rice field surrounding corresponding with the hybrid rice
Male parent restorer is isolated, or can be planted the dry land crop of non-rice crop and be isolated.
In addition, in order to improve the purity of series of three-series hybrid rice seed, it is also necessary to organize artificial rogueing and prevent machine
Tool processing mixes.
Therefore, the purity that traditional triple crossing prepares rice paddy seed, which will receive following factor, to be influenced: the purity of parent, empty
Between isolation distance it is inadequate, because the recognition capability and level of effort of people are different when removal of impurities.
Moreover, to prepare rice paddy seed time-consuming and laborious for traditional triple crossing, it is at high cost.
For double-linear hybrid rice, the production of hybrid seeds is carried out usually using light temperature sensitive type sterile line.Particularly, illumination and temperature are
Influence the production of hybrid seeds the biggest factor, long in illumination, when temperature is high, light temperature sensitive type sterile line shows as male sterility, it is at this moment all just
Ordinary water rice varieties can produce the rice paddy seed for being used for field planting with its normal hybridisation；It is short in illumination, when temperature is low, light temperature
Quick type sterile line shows as normal rice, and self-fertility produces next-generation light temperature sensitive type sterile line.But double-linear hybrid rice
The problem of be: be illuminated by the light, temperature and regional effect it is particularly evident, for example, once in the 3-5 phase of ear differentiation, temperature is low
In critical-temperature, then light temperature sensitive type sterile line will part self-fertility, it is difficult to avoid special weather that seed purity is caused not reach
Mark.In addition, parental purity also will affect double-line hybrid breeding purity.
In conclusion this field still needs the rice paddy seed of high-purity, prepare the method for the rice paddy seed of high-purity with
And it is able to solve and causes the technical solutions of huge economic losses because seed purity is not up to standard.
Summary of the invention
In order to solve the above-mentioned technical problem, it is an object of the present invention to provide a kind of high purity products, for using
The purity of two systems and ternary hybridization method, the product is at least above 97%.It is another object of the present invention to provide systems
The method of the standby high purity product.
Therefore, on the one hand, the present invention provides a kind of rice paddy seed products containing anti-herbicide gene of field planting, should
Product includes the weeding that rice paddy seed containing anti-herbicide gene and the rice paddy seed containing anti-herbicide gene can be resisted
Agent.
On the other hand, the present invention also provides a kind of systems of the rice paddy seed product containing anti-herbicide gene of field planting
Preparation Method, method includes the following steps:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, is either returned with the restorer or male parent
It is one or many, it is either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with CMS line, obtains and is removed containing anti-
The rice paddy seed of careless agent gene；
(4) the herbicide treatment step (3) that can be resisted with the rice paddy seed containing anti-herbicide gene that step (3) obtain
The rice paddy seed containing anti-herbicide gene obtained.
Or
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, is either returned with the restorer or male parent
It is one or many, it is either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with two-line sterile line, obtains and is removed containing anti-
The rice paddy seed of careless agent gene；
(4) the herbicide treatment step (3) that can be resisted with the rice paddy seed containing anti-herbicide gene that step (3) obtain
The rice paddy seed containing anti-herbicide gene obtained.
The rice paddy seed product containing anti-herbicide gene of field planting provided by the invention can guarantee the rice of seedling
Strain purity is at least above 98%, and in a preferred embodiment, the rice strain purity of seedling is greater than or equal to 99%, even
It is 100%.In addition, a kind of preparation method of the rice paddy seed product containing anti-herbicide gene of field planting provided by the invention
It is simple and easy (for example, for ternary hybridization: artificial impurity elimination can be saved；Space interval can suitably reduce, and protect in parent
Holding the purity for being can slightly reduce, so as to solve the problems, such as seed breeding base difficulty implement, can also substantially reduce the production of hybrid seeds at
This；And the sterile line for having part self-fertility unstable in three-line breeding and the production of hybrid seeds, qualified purity can also be prepared
The seed of field planting；For double-line hybrid breeding, the maternal selfing in part caused by can solve due to Thermo-Photo sensitive is unsatisfactory for
Purity excessive problem brought by solid), the rice paddy seed product seedling purity of preparation is very high, can at least reach 98% with
On.Therefore, rice paddy seed product provided by the invention and preparation method thereof can thoroughly solve because seed purity is not up to standard and
Caused huge economic losses problem.
In addition, the rice paddy seed (being free of herbicide) containing anti-herbicide gene of field planting provided by the invention is another
A feature is: herbicide spraying can also kill hybrid strain when rice three is to four leaf stage, but hybrid strain will be in herbicide spraying
Afterwards three weeks or so could be completely withered, if peasant household forgets to spray or sprayed dose and method is incorrect causes hybrid strain incomplete
Dead this can all bring very big adverse effect to agricultural production, also certain pollution can be caused to environment: and use this hair
Bright rice paddy seed product, seed routinely after planting, do not need peasant household and take any measure, rice hybrid strain is unable to seedling, thorough
Bottom solves the problems, such as huge economic losses caused by seed purity is not up to standard；It is removed because being contained using rice paddy seed product of the invention
Seldom (weight ratio of the herbicide is 80-1600:1 to careless dosage, preferably 500-1000:1.) dirt to environment can be reduced
Dye.
Specific embodiment
The present invention provides a kind of rice paddy seed product containing anti-herbicide gene of field planting, which includes containing anti-
The herbicide that the rice paddy seed of herbicide resistance gene and the rice paddy seed containing anti-herbicide gene can be resisted.
The rice paddy seed product containing anti-herbicide gene of field planting provided by the invention can guarantee routinely normal
Sowing does not take seed seedling purity in the case where other any measures to be at least above 98%, thus greatly reduces because of seed
Huge economic losses caused by purity is not up to standard.
What the rice paddy seed containing anti-herbicide gene and the rice paddy seed containing anti-herbicide gene can be resisted
The ratio of herbicide is not particularly limited, as long as the concentration (or content) of the herbicide can satisfy in seedling (or crop field kind
Plant) during kill the young crops that grows of rice paddy seed without anti-herbicide gene.
But in order to kill the young crops that all rice paddy seeds without anti-herbicide gene are grown as far as possible, a kind of excellent
In the embodiment of choosing, the weight ratio of the rice paddy seed containing anti-herbicide gene and the herbicide is 80-1600:1；For
It guarantee herbicide selection effect and reduces environmental pollution as far as possible, it is therefore preferable to the rice seed containing anti-herbicide gene
The weight ratio 500-1000:1 of the sub and described herbicide.
In the present invention, the form of the product is not particularly limited, in a preferred embodiment, the product
For the mixture of the rice paddy seed containing anti-herbicide gene and the herbicide；Or the product is the herbicide
The form of the rice paddy seed of the coated anti-herbicide gene of coating agent；Or the product is the pelletized of the herbicide
The pellet of the rice paddy seed of the coated anti-herbicide gene of agent.
In the present invention, the antiweed is not particularly limited, as long as meeting the rice pair for containing the antiweed
The herbicide has repellence, and in a preferred embodiment, the antiweed is triazine herbicide, nitrile
Herbicide, pyridines herbicide, cyclic imide class herbicide, sulfonamides herbicide, imidazolinone herbicide, sulfonylurea
Herbicide, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；Preferably imidazolinone herbicide, sulfonylurea remove
Careless agent, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；Most preferably imidazolinone herbicide, such as weed eradication
Cigarette, Imazethapyr, hundred ridges are logical.
In a preferred embodiment, the rice paddy seed containing anti-herbicide gene is prepared by following steps:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, is either returned with the restorer or male parent
It is one or many, it is either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with three systems or two-line sterile line, is obtained
The rice paddy seed of anti-herbicide gene must be contained.
In order to improve the purity of crop field rice paddy seed, in a preferred embodiment, carry out step (1) it
Before, with the coating agent for the herbicide that can be resisted containing the rice breed containing anti-herbicide gene to containing antiweed
The rice breed of gene is coated；Or it is removed what the rice breed containing anti-herbicide gene can be resisted
Careless agent is dressed seed with the rice breed containing anti-herbicide gene；Or with containing certain density described containing antiweed
The herbicide solution that the rice breed of gene can be resisted carries out the rice breed containing anti-herbicide gene
It impregnates；Or the rice shoot of the rice breed containing anti-herbicide gene is sprayed containing the rice containing anti-herbicide gene
The herbicide that breeding material can be resisted.To remove the rice without anti-herbicide gene mixed.
The rice breed containing anti-herbicide gene can be it is non-transgenic or transgenosis, specifically can be with
It is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, the Herbicide Resistant Rice that Jiangsu Province Agriculture Science Institute is cultivated, Shenzhen is emerging
The Herbicide Resistant Rice that prosperous biological species industry Co., Ltd cultivates, or the rice containing bar gene.But in a kind of preferred implementation
In mode, the rice breed containing anti-herbicide gene is non-transgenic rice breed, specifically can be beauty
The Herbicide Resistant Rice PT2 that cyanamide company, state cultivates, the Herbicide Resistant Rice that Jiangsu Province Agriculture Science Institute is cultivated, Shenzhen prosperity are raw
The Herbicide Resistant Rice that species industry Co., Ltd cultivates.
The present invention also provides a kind of preparation method of the rice paddy seed product containing anti-herbicide gene of field planting, the party
Method the following steps are included:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, is either returned with the restorer or male parent
It is one or many, it is either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with three systems or two-line sterile line, is obtained
The rice paddy seed of anti-herbicide gene must be contained；
(4) the herbicide treatment step (3) that can be resisted with the rice paddy seed containing anti-herbicide gene that step (3) obtain
The rice paddy seed containing anti-herbicide gene obtained.
In step (4), the rice paddy seed and the rice seed containing anti-herbicide gene containing anti-herbicide gene
The ratio for the herbicide that son can be resisted is not particularly limited, as long as the concentration (or content) of the herbicide can satisfy kill
The young crops that rice paddy seed during seedling (or field planting) without anti-herbicide gene is grown.But in order to kill as far as possible
The young crops that all rice paddy seeds without anti-herbicide gene are grown, it is in a preferred embodiment, described to contain anti-weeding
The weight ratio of the rice paddy seed of agent gene and the herbicide is 80-1600:1；In order to guarantee herbicide selection effect and use up
It is likely to reduced environmental pollution, it is therefore preferable to the weight ratio 500- of the rice paddy seed containing anti-herbicide gene and the herbicide
1000:1.
In order to improve the strain purity of crop field seed seedling as far as possible, in a preferred embodiment, in step (4)
In: the rice paddy seed containing anti-herbicide gene and the herbicide are dressed seed；Or the coating with the herbicide
Agent is coated with the rice paddy seed of the anti-herbicide gene；Or the antiweed base is coated with the pelleting agent of the herbicide
The rice paddy seed of cause.The rice paddy seed in treated in this way crop field can kill as far as possible during seedling (or field planting)
Dead all rice young crops without anti-herbicide gene.
In order to improve the purity of crop field rice paddy seed, in a preferred embodiment, carry out step (1) it
Before, with the coating agent for the herbicide that can be resisted containing the rice breed containing anti-herbicide gene to containing antiweed
The rice breed of gene is coated；Or it will can be resisted containing the rice breed containing anti-herbicide gene
Herbicide dress seed with the rice breed containing anti-herbicide gene；Or it described is removed containing anti-with containing certain density
The herbicide solution that the rice breed of careless agent gene can be resisted is to the rice breed containing anti-herbicide gene
It is impregnated；Or the rice shoot of the rice breed containing anti-herbicide gene is sprayed containing described containing anti-herbicide gene
The herbicide that rice breed can be resisted.To remove the rice without anti-herbicide gene mixed.
The rice breed containing anti-herbicide gene can be it is non-transgenic or transgenosis, specifically can be with
It is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, the Herbicide Resistant Rice that Jiangsu Province Agriculture Science Institute is cultivated, Shenzhen is emerging
The Herbicide Resistant Rice that prosperous biological species industry Co., Ltd cultivates, or the rice containing bar gene.But in a kind of preferred implementation
In mode, the rice breed containing anti-herbicide gene is non-transgenic rice breed, specifically can be beauty
The Herbicide Resistant Rice PT2 that cyanamide company, state cultivates, the Herbicide Resistant Rice that Jiangsu Province Agriculture Science Institute is cultivated, Shenzhen prosperity are raw
The Herbicide Resistant Rice that species industry Co., Ltd cultivates.
In the present invention, the antiweed is not particularly limited, as long as meeting the rice pair for containing the antiweed
The herbicide has repellence, and in a preferred embodiment, the antiweed is triazine herbicide, nitrile
Herbicide, pyridines herbicide, cyclic imide class herbicide, sulfonamides herbicide, imidazolinone herbicide, sulfonylurea
Herbicide, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；Preferably imidazolinone herbicide, sulfonylurea remove
Careless agent, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；Most preferably imidazolinone herbicide, such as weed eradication
Cigarette, Imazethapyr, hundred ridges are logical.
Embodiment
1 double-line hybrid breeding of embodiment
(1) (made Shenzhen with refined extensive 2115 (being introduced from Sichuan Agricultural University) and the Huang Huazhan containing anti-herbicide gene
Object Molecular design breeding research institute) preparing hybrid combination.Refined extensive 2115 and the Huang Huazhan containing anti-herbicide gene are sowed, to 3-
The Huang Huazhan containing anti-herbicide gene of 4 leaf phases sprays hundred ridges and leads to (0.4 volume % of concentration), hybridizes and F1 generation is made.
(2) the BC2 generation containing anti-herbicide gene by F1 generation and refined extensive 2115 backcrossing until obtaining stabilization characteristics of genetics.
(3) in BC2 generation, is hybridized with two-line sterile line Y58S (introducing from Hunan Research Centre for Hybrid Rice), is contained
There is the rice paddy seed of the field planting of antiweed.
(4) it is dressed seed with the rice paddy seed of the logical field planting obtained with step (3) in hundred ridges, wherein hundred ridges are logical and big
The weight ratio of the rice paddy seed of field plantation is that (per kilogram seed leads to ((effective component: 240g/L first imidazoles cigarette with hundred ridges to 1:1000
Sour water agent)) ((4.17ml)+tap water (21ml)) dress seed.
The detection of seed purity and young crops purity:
600 are taken to be divided into A and two groups of B the rice paddy seed of the field planting obtained in 1 step of embodiment (3), from
The rice paddy seed product of the field planting obtained in 1 step of embodiment (4) takes 300, forms C group.Routinely simple grain after vernalization
Sowing, A group spray clear water in 3 leaf phase herbicide sprayings (0.4 volume % of concentration), 3 leaf phase of B group.It is counted for the first time in the 3 leaf phase of rice shoot
The quantity of number seedling carries out second of the quantity for counting seedling in 20 days after first time counts, for the third time in heading poplar bloassom phase meter
The type and quantity of number hybrid strain.As a result as follows:
Conclusion: 1, the seed field germination percentage (planting percent) of A group and B group up to 97.3% (national standard is >=80%), C group
Seed field germination percentage (planting percent) be 93.7%.
2, it is 96.2% (national standard is >=96%) by the seed purity that step (3) provide, does not spray weeding in 3 leaf phases
Agent is 96.2% by the young crops purity that the seed that step (3) provide obtains；In 3 leaf phase herbicide sprayings, provided by step (3)
Seed obtain young crops purity be 100%.
3, the seed products and young crops purity provided by step (4) are 100% (national standard is >=96%).
In addition, hybrid strain will the just dead (emergence at after planting 40 days or so after the seed herbicide spraying that step (3) obtains
It is dead afterwards)；If peasant household forget to spray or sprayed dose and method it is incorrect according at hybrid strain not exclusively it is dead this all can be to agriculture
Industry production, which brings very big adverse effect, also can cause certain pollution to environment: and the seed products that step (4) obtains are pressed
Hybrid strain all death (not emerging) after conventional seed planting.Therefore, using seed products provided by the invention and young crops purity is high, soil
Utilization rate is high, and environmental pollution is few
2 double-line hybrid breeding of embodiment
With refined extensive 2115 (being introduced from Sichuan Agricultural University) and Huang Huazhan (the Shenzhen crop point containing anti-herbicide gene
Sub- design and context research institute) preparing hybrid combination.Contain anti-herbicide gene with logical impregnate in hundred ridges containing 0.2 volume % of concentration
Yellow China account for 24 hours, sowing refined extensive 2115 and the Huang Huazhan containing anti-herbicide gene after being impregnated with Arsenal, hybridization system
Obtain F1 generation.
(2) the BC2 generation containing anti-herbicide gene by F1 generation and refined extensive 2115 backcrossing until obtaining stabilization characteristics of genetics.
(3) in BC2 generation, is hybridized with two-line sterile line Y58S (introducing from Hunan Research Centre for Hybrid Rice), obtains and contains anti-weeding
The rice paddy seed of the field planting of agent.
(4) rice paddy seed of the field planting obtained with coating agent coating step (3) led to containing hundred ridges, wherein hundred ridges
The logical weight ratio with the rice paddy seed of field planting is 1:1600.
Wherein, the preparation process of coating agent is as follows: for one kilogram of seed, by (the You Zhonghua crop protection of 2.5ml film forming agent
Product Co., Ltd provides), 2.1ml herbicide (240g/L AC 263222 aqua) and 21ml tap water are uniformly mixed.
The detection of seed purity and young crops purity:
600 are taken to be divided into A and two groups of B the rice paddy seed of the field planting obtained in 2 step of embodiment (3), from
The rice paddy seed product of the field planting obtained in 2 step of embodiment (4) takes 300, forms C group.Routinely simple grain after vernalization
Sowing, A group spray clear water in 3 leaf phase herbicide sprayings (0.4 volume % of concentration), 3 leaf phase of B group.It is counted for the first time in the 3 leaf phase of rice shoot
The quantity of number seedling carries out second of the quantity for counting seedling in 20 days after first time counts, for the third time in heading poplar bloassom phase meter
The quantity of number hybrid strain.As a result as follows:
Conclusion: 1, the seed field germination percentage (planting percent) of A group and B group up to 94.7% (national standard is >=80%), C group
Seed field germination percentage (planting percent) be 88.3%；
2, it is 93.3% (national standard is >=96%) by the seed purity that step (3) provide, does not spray weeding in 3 leaf phases
Agent is 93.3% by the young crops purity that the seed that step (3) provide obtains；In 3 leaf phase herbicide sprayings, provided by step (3)
Seed obtain young crops purity be 100%.
3, the seed products and young crops purity provided by step (4) are 100% (national standard is >=96%)
In addition, hybrid strain will the just dead (emergence at after planting 40 days or so after the seed herbicide spraying that step (3) obtains
It is dead afterwards)；If peasant household forget to spray or sprayed dose and method it is incorrect according at hybrid strain not exclusively it is dead this all can be to agriculture
Industry production, which brings very big adverse effect, also can cause certain pollution to environment: and the seed products that step (4) obtains are pressed
Hybrid strain all death (not emerging) after conventional seed planting.Therefore, using seed products provided by the invention and young crops purity is high, soil
Utilization rate is high.Environmental pollution is few.
3 double-line hybrid breeding of embodiment
(1) (made Shenzhen with refined extensive 2115 (being introduced from Sichuan Agricultural University) and the Huang Huazhan containing anti-herbicide gene
Object Molecular design breeding research institute) preparing hybrid combination, seed dressing is mixed with logical account for the yellow China containing anti-herbicide gene in hundred ridges
(per kilogram seed leads to ((effective component: 240g/L AC 263222 aqua)) ((13.9ml)+water (21ml)) with hundred ridges, sowing
Refined extensive 2115 and with weed eradication mixing seed dressing after the Huang Huazhan containing anti-herbicide gene, hybridize be made F1 generation.
(2) the BC2 generation containing anti-herbicide gene by F1 generation and refined extensive 2115 backcrossing until obtaining stabilization characteristics of genetics.
(3) in BC2 generation, is hybridized with two-line sterile line Y58S (introducing from Hunan Research Centre for Hybrid Rice), is contained
There is the rice paddy seed of the field planting of antiweed.
(4) it is dressed seed with the rice paddy seed of the logical field planting obtained with step (3) in hundred ridges, wherein hundred ridges are logical and big
The weight ratio of the rice paddy seed of field plantation is that (per kilogram seed leads to ((effective component: 240g/L AC 263222 with hundred ridges to 1:300
Aqua)) ((13.9ml)+tap water (21ml)) dress seed.
The detection of seed purity and young crops purity:
600 are taken to be divided into A and two groups of B the rice paddy seed of the field planting obtained in 3 step of embodiment (3), from
The rice paddy seed product of the field planting obtained in 3 step of embodiment (4) takes 300, forms C group.Routinely simple grain after vernalization
Sowing, A group spray clear water in 3 leaf phase herbicide sprayings (0.4 volume % of concentration), 3 leaf phase of B group.It is counted for the first time in the 3 leaf phase of rice shoot
The quantity of number seedling carries out second of the quantity for counting seedling in 20 days after first time counts, for the third time in heading poplar bloassom phase meter
The quantity of number hybrid strain.As a result as follows:
Conclusion: 1, the seed field germination percentage (planting percent) of A group and B group up to 92.7% (national standard is >=80%), C group
Seed field germination percentage (planting percent) be 82.6%.
2, it is 89.2% (national standard is >=96%) by the seed purity that step (3) provide, does not spray weeding in 3 leaf phases
Agent is 89.2% by the young crops purity that the seed that step (3) provide obtains；In 3 leaf phase herbicide sprayings, provided by step (3)
Seed obtain young crops purity be 99.3%.
3, the seed products and young crops purity provided by step (4) are 100% (national standard is >=96%)
In addition, hybrid strain will the just dead (emergence at after planting 40 days or so after the seed herbicide spraying that step (3) obtains
It is dead afterwards)；If peasant household forget to spray or sprayed dose and method it is incorrect according at hybrid strain not exclusively it is dead this all can be to agriculture
Industry production brings very big adverse effect can also cause certain pollution to environment；And the seed products that step (4) obtains are pressed
Hybrid strain all death (not emerging) after conventional seed planting.Therefore, using seed products provided by the invention and young crops purity is high, soil
Utilization rate is high, and environmental pollution is few.
4 ternary hybridization of embodiment
(1) with R198 (commercially available, Sichuan Zheng Xingzhong industry Co., Ltd) and (Shenzhen Huang Huazhan containing anti-herbicide gene
Perspectives of Molecular Design Breeding in Crops research institute) preparing hybrid combination.The R198 and Huang Huazhan containing anti-herbicide gene is sowed, to 3-4
The Huang Huazhan containing anti-herbicide gene of leaf phase is sprayed Arsenal (0.4 volume % of concentration), hybridizes and F1 generation is made.
(2) F1 generation and R198 are returned to the BC2 generation containing anti-herbicide gene until obtaining stabilization characteristics of genetics.
(3) in BC2 generation, is hybridized with CMS line dragon 1A (introducing from Sichuan Ao Lixing agricultural science and technology Co., Ltd),
Obtain the rice paddy seed of the field planting containing antiweed.
(4) rice paddy seed of the field planting obtained with Arsenal with step (3) is dressed seed, wherein Arsenal and big
The weight ratio of the rice paddy seed of field plantation is 1:500 (per kilogram seed Arsenal ((Niacin Nicitinic Acid -2- [4,5- dihydro -4-
Methyl -4- (1- Methylethyl) -5- oxygen -1H- imidazoles -2- base] ester, CAS 81334-34-1) (concentration is aqua
0.345%))) ((5.8ml)+tap water (21ml)).
The detection of seed purity and young crops purity:
600 are taken to be divided into A and two groups of B the rice paddy seed of the field planting obtained in 4 step of embodiment (3), from
The rice paddy seed product of the field planting obtained in 4 step of embodiment (4) takes 300, forms C group.Routinely simple grain after vernalization
Sowing, A group spray clear water in 3 leaf phase herbicide sprayings (0.4 volume % of concentration), 3 leaf phase of B group.It is counted for the first time in the 3 leaf phase of rice shoot
The quantity of number seedling carries out second of the quantity for counting seedling in 20 days after first time counts, for the third time in heading poplar bloassom phase meter
The quantity of number hybrid strain.As a result as follows:
Conclusion: 1, the seed field germination percentage (planting percent) of A group and B group up to 93.3% (national standard is >=80%), C group
Seed field germination percentage (planting percent) be 85%.
2, it is 91% (national standard is >=96%) by the seed purity that step (3) provide, does not spray weeding in 3 leaf phases
Agent is 91% by the young crops purity that the seed that step (3) provide obtains；In 3 leaf phase herbicide sprayings, provided by step (3)
The young crops purity that seed obtains is 99.6%.
3, the seed products and young crops purity provided by step (4) are 100% (national standard is >=96%)
In addition, hybrid strain will the just dead (emergence at after planting 40 days or so after the seed herbicide spraying that step (3) obtains
It is dead afterwards)；If peasant household forget to spray or sprayed dose and method it is incorrect according at hybrid strain not exclusively it is dead this all can be to agriculture
Industry production brings very big adverse effect can also cause certain pollution to environment；And the seed products that step (4) obtains are pressed
Hybrid strain all death (not emerging) after conventional seed planting.Therefore, using seed products provided by the invention and young crops purity is high, soil
Utilization rate is high.Environmental pollution is few.
Claims (26)
1. a kind of rice paddy seed product containing anti-herbicide gene of field planting, which is characterized in that the product includes removing containing anti-
The herbicide that the rice paddy seed of careless agent gene and the rice paddy seed containing anti-herbicide gene can be resisted.
2. the rice paddy seed product containing anti-herbicide gene of field planting according to claim 1, which is characterized in that institute
The weight ratio for stating the rice paddy seed containing anti-herbicide gene and the herbicide is 80-1600:1, preferably 500-1000:1.
3. the rice paddy seed product containing anti-herbicide gene of field planting according to claim 1, which is characterized in that institute
State the mixture that product is the rice paddy seed and the herbicide containing anti-herbicide gene；Or the product is described removes
The form of the rice paddy seed of the coated anti-herbicide gene of the coating agent of careless agent；Or the product is the herbicide
The pellet of the rice paddy seed of the coated anti-herbicide gene of pelleting agent.
4. the rice paddy seed product containing anti-herbicide gene of field planting according to claim 1, which is characterized in that institute
Stating antiweed is triazine herbicide, nitrile herbicide, pyridines herbicide, cyclic imide class herbicide, sulfonamides
Herbicide, imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；
Preferably imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidinylthiobenzoate herbicides；Most
Preferably imidazolinone herbicide.
5. the rice paddy seed product containing anti-herbicide gene of field planting described in any one of -4 according to claim 1,
It is characterized in that, the rice paddy seed containing anti-herbicide gene is prepared by following steps:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, either primary with the restorer or male parent backcrossing
Or repeatedly, either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with CMS line, obtains and contains antiweed
The rice paddy seed of gene.
6. the rice paddy seed product according to claim 5 containing anti-herbicide gene, which is characterized in that carrying out step
(1) before, with the coating agent for the herbicide that can be resisted containing the rice breed containing anti-herbicide gene to containing anti-
The rice breed of herbicide resistance gene is coated；Or the rice breed containing anti-herbicide gene can be supported
Anti- herbicide is dressed seed with the rice breed containing anti-herbicide gene；Or with containing certain density described containing anti-
The herbicide solution that the rice breed of herbicide resistance gene can be resisted is to the rice breeding material containing anti-herbicide gene
Material is impregnated；Or the rice shoot of the rice breed containing anti-herbicide gene is sprayed containing described containing anti-herbicide gene
The herbicide that can resist of rice breed.
7. the rice paddy seed product according to claim 5 containing anti-herbicide gene, which is characterized in that described to contain anti-weeding
The rice breed of agent gene is non-transgenic rice breed.
8. the rice paddy seed product according to claim 5 containing anti-herbicide gene, which is characterized in that described to contain anti-weeding
The rice breed of agent gene is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, and Jiangsu Province Agriculture Science Institute is cultivated
Herbicide Resistant Rice, the Herbicide Resistant Rice that Shenzhen Xingwang Biological Seed Industry Co., Ltd. cultivates, or the water containing bar gene
Rice.
9. the rice paddy seed product containing anti-herbicide gene of field planting described in any one of -4 according to claim 1,
It is characterized in that, the rice paddy seed containing anti-herbicide gene is prepared by following steps:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, either primary with the restorer or male parent backcrossing
Or repeatedly, either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with two-line sterile line, obtains and contains antiweed
The rice paddy seed of gene.
10. the rice paddy seed product according to claim 9 containing anti-herbicide gene, which is characterized in that carrying out step
(1) before, with the coating agent for the herbicide that can be resisted containing the rice breed containing anti-herbicide gene to containing anti-
The rice breed of herbicide resistance gene is coated；Or the rice breed containing anti-herbicide gene can be supported
Anti- herbicide is dressed seed with the rice breed containing anti-herbicide gene；Or with containing certain density described containing anti-
The herbicide solution that the rice breed of herbicide resistance gene can be resisted is to the rice breeding material containing anti-herbicide gene
Material is impregnated；Or the rice shoot of the rice breed containing anti-herbicide gene is sprayed containing described containing anti-herbicide gene
The herbicide that can resist of rice breed.
11. the rice paddy seed product according to claim 9 containing anti-herbicide gene, which is characterized in that described to be removed containing anti-
The rice breed of careless agent gene is non-transgenic rice breed.
12. the rice paddy seed product according to claim 9 containing anti-herbicide gene, which is characterized in that described to be removed containing anti-
The rice breed of careless agent gene is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, Jiangsu Province Agriculture Science Institute training
The Herbicide Resistant Rice educated, the Herbicide Resistant Rice that Shenzhen Xingwang Biological Seed Industry Co., Ltd. cultivates, or contain bar gene
Rice.
13. a kind of preparation method of the rice paddy seed product containing anti-herbicide gene of field planting, which is characterized in that this method
The following steps are included:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, either primary with the restorer or male parent backcrossing
Or repeatedly, either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with CMS line, obtains and contains antiweed
The rice paddy seed of gene；
(4) it is obtained with the herbicide treatment step (3) that the rice paddy seed containing anti-herbicide gene that step (3) obtain can be resisted
The rice paddy seed containing anti-herbicide gene.
14. the preparation method of the rice paddy seed product containing anti-herbicide gene of field planting according to claim 13,
It is characterized in that, the weight ratio of the rice paddy seed containing anti-herbicide gene and the herbicide is 80- in step (4)
1600:1, preferably 500-1000:1.
15. the preparation side of the rice paddy seed product containing anti-herbicide gene of field planting described in 3 or 14 according to claim 1
Method, which is characterized in that in step (4): the rice paddy seed containing anti-herbicide gene and the herbicide are dressed seed；
Or the rice paddy seed of the anti-herbicide gene is coated with the coating agent of the herbicide；Or the pellet with the herbicide
Agent is coated with the rice paddy seed of the anti-herbicide gene.
16. the preparation side of the rice paddy seed product containing anti-herbicide gene of field planting described in 3 or 14 according to claim 1
Method, which is characterized in that before carrying out step (1), can be supported with containing the rice breed containing anti-herbicide gene
The coating agent of anti-herbicide is coated the rice breed containing anti-herbicide gene；Or it will be removed containing described containing anti-
The herbicide that the rice breed of careless agent gene can be resisted is dressed seed with the rice breed containing anti-herbicide gene；
Or with the herbicide solution that can be resisted containing the certain density rice breed containing anti-herbicide gene to institute
The rice breed containing anti-herbicide gene is stated to be impregnated；Or the seedling to the rice breed containing anti-herbicide gene
Seedling sprays the herbicide that can be resisted containing the rice breed containing anti-herbicide gene.
17. the preparation method of the rice paddy seed product described in 3 or 14 containing anti-herbicide gene according to claim 1, feature
It is, the rice breed containing anti-herbicide gene is non-transgenic rice breed.
18. the preparation method of the rice paddy seed product described in 3 or 14 containing anti-herbicide gene according to claim 1, feature
It is, the rice breed containing anti-herbicide gene is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, Jiangsu
The Herbicide Resistant Rice that Shanxi Academy of Agricultural Sciences cultivates, the Herbicide Resistant Rice that Shenzhen Xingwang Biological Seed Industry Co., Ltd. cultivates, or
Person contains the rice of bar gene.
19. the preparation method of the rice paddy seed product described in 3 or 14 containing anti-herbicide gene according to claim 1, feature
It is, the antiweed is triazine herbicide, nitrile herbicide, pyridines herbicide, cyclic imide class herbicide, sulphur
Acetamide-group herbicides, imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidine salicylic acid remove
Careless agent；Preferably imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidine salicylic acid weeding
Agent；Most preferably imidazolinone herbicide.
20. a kind of preparation method of the rice paddy seed product containing anti-herbicide gene of field planting, which is characterized in that this method
The following steps are included:
(1) restorer or male parent are hybridized with the rice breed containing anti-herbicide gene；
(2) the rice paddy seed selfing obtained step (1) is one or many, either primary with the restorer or male parent backcrossing
Or repeatedly, either primary or multiple with other mixing breeds；And
(3) strain containing anti-herbicide gene that step (2) obtain is hybridized with two-line sterile line, obtains and contains antiweed
The rice paddy seed of gene；
(4) it is obtained with the herbicide treatment step (3) that the rice paddy seed containing anti-herbicide gene that step (3) obtain can be resisted
The rice paddy seed containing anti-herbicide gene.
21. the preparation method of the rice paddy seed product containing anti-herbicide gene of field planting according to claim 20,
It is characterized in that, the weight ratio of the rice paddy seed containing anti-herbicide gene and the herbicide is 80- in step (4)
1600:1, preferably 500-1000:1.
22. the preparation side of the rice paddy seed product containing anti-herbicide gene of the field planting according to claim 20 or 21
Method, which is characterized in that in step (4): the rice paddy seed containing anti-herbicide gene and the herbicide are dressed seed；
Or the rice paddy seed of the anti-herbicide gene is coated with the coating agent of the herbicide；Or the pellet with the herbicide
Agent is coated with the rice paddy seed of the anti-herbicide gene.
23. the preparation side of the rice paddy seed product containing anti-herbicide gene of the field planting according to claim 20 or 21
Method, which is characterized in that before carrying out step (1), can be supported with containing the rice breed containing anti-herbicide gene
The coating agent of anti-herbicide is coated the rice breed containing anti-herbicide gene；Or it will be removed containing described containing anti-
The herbicide that the rice breed of careless agent gene can be resisted is dressed seed with the rice breed containing anti-herbicide gene；
Or with the herbicide solution that can be resisted containing the certain density rice breed containing anti-herbicide gene to institute
The rice breed containing anti-herbicide gene is stated to be impregnated；Or the seedling to the rice breed containing anti-herbicide gene
Seedling sprays the herbicide that can be resisted containing the rice breed containing anti-herbicide gene.
24. the preparation method of the rice paddy seed product according to claim 20 or 21 containing anti-herbicide gene, feature
It is, the rice breed containing anti-herbicide gene is non-transgenic rice breed.
25. the preparation method of the rice paddy seed product according to claim 20 or 21 containing anti-herbicide gene, feature
It is, the rice breed containing anti-herbicide gene is the Herbicide Resistant Rice PT2 that American Cyanamid Company cultivates, Jiangsu
The Herbicide Resistant Rice that Shanxi Academy of Agricultural Sciences cultivates, the Herbicide Resistant Rice that Shenzhen Xingwang Biological Seed Industry Co., Ltd. cultivates, or
Person contains the rice of bar gene.
26. the preparation method of the rice paddy seed product according to claim 20 or 21 containing anti-herbicide gene, feature
It is, the antiweed is triazine herbicide, nitrile herbicide, pyridines herbicide, cyclic imide class herbicide, sulphur
Acetamide-group herbicides, imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidine salicylic acid remove
Careless agent；Preferably imidazolinone herbicide, sulfonylurea herbicide, triazolo pyrimidine class herbicide and pyrimidine salicylic acid weeding
Agent；Most preferably imidazolinone herbicide.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2017108969731 | 2017-09-28 | ||
| CN201710896973 | 2017-09-28 | ||
| CN201710953101.4A CN107581057A (en) | 2017-09-28 | 2017-10-13 | Hybrid rice breeding method, hybrid rice seed breeding process and seed coat agent |
| CN2017109531014 | 2017-10-13 | ||
| CN201810617736.1A CN109006452B (en) | 2017-09-28 | 2018-06-15 | A kind of rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
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| CN110291986A true CN110291986A (en) | 2019-10-01 |
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| CN201910700270.6A Pending CN110291986A (en) | 2017-09-28 | 2018-06-15 | Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
| CN201810617736.1A Active CN109006452B (en) | 2017-09-28 | 2018-06-15 | A kind of rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
| CN201910700839.9A Pending CN110235779A (en) | 2017-09-28 | 2018-06-15 | The rice paddy seed product containing anti-herbicide gene of field planting |
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| CN201910700839.9A Pending CN110235779A (en) | 2017-09-28 | 2018-06-15 | The rice paddy seed product containing anti-herbicide gene of field planting |
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| CN107581057A (en) | 2018-01-16 |
| CN110235779A (en) | 2019-09-17 |
| CN109006452A (en) | 2018-12-18 |
| CN109006452B (en) | 2019-07-26 |
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