CN1247699A - Chemical roguing technology for breeding hybrid rice - Google Patents
Chemical roguing technology for breeding hybrid rice Download PDFInfo
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- CN1247699A CN1247699A CN 99115125 CN99115125A CN1247699A CN 1247699 A CN1247699 A CN 1247699A CN 99115125 CN99115125 CN 99115125 CN 99115125 A CN99115125 A CN 99115125A CN 1247699 A CN1247699 A CN 1247699A
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Abstract
The chemical rogueing technology for breeding hybrid rice includes introducing anti-herbicide gene into the maintainer line or three-line hybrid rice to make the maintainer line possess anti-herbicide characteristics; crossbreeding sterile line with anti-herbicide characteristics with the maintainer line and one sterile line with a same type and no anti-herbicide gene; and crossbreeding three-line hybrid rice seed with the sterile line with anti-herbicide characteristics and the maintainer line. By means of the technology, it is possible to breed hybrid rice with high purity and to eliminate the manual rogueing during breeding.
Description
What the present invention relates to is a kind of chemical roguing technology for breeding hybrid rice.
The breeding and promoting of China hybrid rice, the application that has lasted two more than ten years, particularly three series mating breeding and seeding technique be extended to China's increasing production of rice and make a great contribution.Worldwide Rice Production has all been produced far-reaching influence.But in hybrid rice production of hybrid seeds process, cause that very easily bio-mechanical mixes.As mixing 4% pseudostationary, can not sell in the hybrid rice seed just be defective seed according to China's pertinent regulations.At present,, breed the production of hybrid seeds, all need to take many anti-assorted, impurity elimination measures, spend a large amount of manually, and the seed quality problem often occurs from close wood for guaranteeing the purity of the ternary hybrid rice production of hybrid seeds, the cost that has increased the hybrid rice production of hybrid seeds greatly with cause heavy losses.
In view of above reason, the objective of the invention is provides a kind of chemical roguing technology for breeding hybrid rice of producing highly purified qualified hybrid rice seed in order to overcome above shortcoming.
The technology of the present invention is the maintenance line that 1. anti-herbicide gene is imported ternary hybrid rice, makes maintenance line obtain anti-herbicide gene, possesses the characteristic of antiweed; 2. become production of hybrid seeds male sterile line with maintenance line with the male sterile line outbreeding that does not contain anti-herbicide gene of homotype, make the male sterile line of breeding have the antiweed characteristic; 3. with the male sterile line in 2. with antiweed characteristic with recover system's hybridization and produce trilinear hybrid rice seed (referring to Fig. 1～Fig. 8).
Above-mentioned anti-herbicide gene adopts optionally non-broad-spectrum herbicide gene.
Above-mentioned optionally non-wide spectrum anti-herbicide gene is the bar gene.
Above-mentioned optionally non-wide spectrum anti-herbicide gene is the aroA gene.
Above-mentioned transgenic maintainer line is three to be the transgenosis stable strain of the maintenance line of male sterile line.
Above-mentioned technology employing particle bombardment or agriculture bacillus mediated conversion method or electrization or its conversion method or ultrasonic conversion method or conventional breeding hybridization transformation method import anti-herbicide gene the maintenance line of ternary hybrid rice
Anti-herbicide gene is optionally non-wide spectrum anti-herbicide genes such as bar gene, aroA gene.Wherein the bar gene separates acquisition from moist streptomycete (Streptomyces hygroscopicus).(the Phosphinothricin transacetylase PAT) can make PPT class weed killer herbicide (Phosphin-othricin or Glufosinate) by the acetylization inactivation to the effect product of this gene, thereby removes PPT toxicity.This gene has been applied to the Study on Transformation of various plants, is the selection markers gene of producing the high-efficient transgenic plant.The aroA gene separates acquisition from Salmonella typhimurium.Behind this gene transfered plant body, the Epsps (5-enolypyruvylshikimate-3-phophatesynthase) of a kind of modification property of coding, because of the affinity of it and herbicide glyphosate (a kind of reactive compound among the weed killer herbicide Roundup+M that U.S. MOnsanto company produces) descends, thereby show resistance to glyphosate.And the toxicity of glyphosate is owing to suppress the activity of Epsps (5-enolypyruvylshikimate-3-phophatesynthase), cause the accumulation of thick grass hydrochlorate (shikimate), and the thick grass hydrochlorate suppresses the synthetic and secondary metabolism of aromatic amino acid, and then causes cell death.The technology of the present invention is when the production of hybrid seeds, can remove non-sterility seedling to the male sterile line herbicide spraying of seed farm, be in the sterile line propagation process because the maintenance line of the no Herbicid resistant that do not stay only of impurity elimination or field seedling, because mixing and the seed farm seedling of producing of the string powder of the paddy rice of the no Herbicid resistant that isolating problem causes, save artificial impurity elimination expenses a large amount of in the production of hybrid seeds process and avoid because the untimely seed quality problem that causes of impurity elimination, not only saving of labor but also can produce the high-purity hybrid seed greatly reduced production of hybrid seeds risk.
The technology of the present invention has following advantage:
1, to the sterility of recovering system's hybridization usefulness at the seedling spraying weed killer herbicide, can in time remove non-male sterile line seedling, for the production of hybrid rice seed provides highly purified male sterile line rice shoot.
2, use the seed purity of hybrid rice height that the technology of the present invention is produced, can be up to 100%, and can improve hybrid seed yield, reduce breeding cost, avoid hybrid rice seed not cause heavy losses to agricultural production, can bring into play hybrid vigour better, to a greater extent because of purity reaches mark.
Introduce embodiments of the invention below:
Fig. 1 is chemical roguing technology for breeding hybrid rice flow chart 1 (numerous kind of flow chart of parent).
Fig. 2 is chemical roguing technology for breeding hybrid rice flow chart 2 (hybrid rice production of hybrid seeds figure).
Fig. 3 is numerous kind of figure of parent of preparation II-excellent 63.
Fig. 4 is the production of hybrid seeds figure of preparation II-excellent 63.
Fig. 5 is the numerous kind of figure of parent on preparation ridge excellent 881.
Fig. 6 is the production of hybrid seeds figure on preparation ridge excellent 881.
Fig. 7 prepares numerous kind of figure of parent of D excellent 68.
Fig. 8 is the production of hybrid seeds figure of preparation D excellent 68.
Fig. 1, Fig. 2 are respectively numerous kind of flow chart of parent, the production of hybrid seeds figure of breeding of hybrid rice chemistry acrobatics art.
Embodiment 1:
Fig. 3, Fig. 4 have provided excellent 63 figure of embodiment of the invention 1-preparation II-.Changing anti-herbicide gene bar gene over to three is that maintenance line II-32B (II-32B1) makes II-32B1 obtain anti-herbicide gene (II-32B2), possesses anti-weeding PPT agent＜characteristic; Three is the II-32A (II-32A12) that male sterile line II-32A (II-32A1) outbreeding becomes to have antiweed PPT characteristic with II-32B2 with what do not contain the weed killer herbicide gene; Be that recovery is that excellent 63 (PPT removes non-male sterile line seedling to II-32A12 seedling spraying weed killer herbicide) of ternary hybrid rice high-purity seed II-are produced in bright extensive 63 hybridization with II-32A12 and three again.When the production of hybrid seeds, can remove non-male sterile line to the II-32A12 herbicide spraying PPT in the seed farm.
II-32A1 among Fig. 3, Fig. 4 is not for containing the II-32A of antiweed bar gene, and II-32B1 is not for containing the II-32B of antiweed bar gene, and II-32A2 is the II-32B that contains antiweed bar gene.
Embodiment 2:
Fig. 5, Fig. 6 have provided embodiment 2-preparation excellent 881 figure in ridge.Changing anti-herbicide gene bar gene over to three is to keep ridge 46B (ridge 46B1), makes ridge 46B1 obtain anti-herbicide gene (ridge 46B2), possesses the characteristic of antiweed PPT; Three is the Gang No.46A (Gang No.46A 12) that male sterile line Gang No.46A A (being Gang No.46A 1) outbreeding becomes to have antiweed PPT characteristic with ridge 46B2 with what do not contain the weed killer herbicide gene; Be that recovery is that excellent 881 (12 seedling spraying weed killer herbicide PPT remove non-male sterile line seedling to Gang No.46A) in trilinear hybrid rice seed ridges are produced in extensive 88 hybridization in another name for Sichuan Province with Gang No.46A 12 and three again.When the production of hybrid seeds, 12 herbicide spraying PPT can remove non-male sterile line to the Gang No.46A in the seed farm.
Gang No.46A 1 among Fig. 5, Fig. 6 is not for containing the Gang No.46A of antiweed bar gene, and ridge 46B1 is not for containing the ridge 46B of antiweed bar gene, and ridge 46B2 is the ridge 46B that contains antiweed bar gene.
Embodiment 3:
Fig. 7, Fig. 8 have provided the excellent 6B figure of embodiment 3-preparation D.Changing anti-herbicide gene bar gene over to three is maintenance line D62B (D62B1), makes D62B1 obtain anti-herbicide gene, is D62B2) possess the characteristic of antiweed PPT; With D62B2 with not contain three of anti-herbicide gene is male sterile line D62A, i.e. D62A1, the D62A that outbreeding becomes to have antiweed characteristic PPT characteristic, i.e. B62A12; Be to recover No. 1 hybridization of polyphyly to produce excellent 68 (PPT removes non-male sterile line seedling to D62A12 spraying herbicide in seedling stage) of trilinear hybrid rice seed D with D62A12 and three again.When the production of hybrid seeds, the DD62A12 herbicide spraying PPT in the seed farm can remove non-male sterile line.
D62A1 among Fig. 7, Fig. 8 is not for containing the D62A of antiweed bar gene, and D62B1 is not for containing the D62B of antiweed bar gene, and D62B2 is the D62B that contains antiweed bar gene.
Claims (6)
1, chemical roguing technology for breeding hybrid rice.This technology is the maintenance line that 1. anti-herbicide gene is imported ternary hybrid rice, makes maintenance line obtain anti-herbicide gene, possesses the characteristic of antiweed; 2. become production of hybrid seeds male sterile line with maintenance line with the male sterile line outbreeding that does not contain anti-herbicide gene of homotype, make the male sterile line of breeding have the antiweed characteristic; 3. the male sterile line with antiweed characteristic in using 2. is a hybridization production trilinear hybrid rice seed with recovering.
2, chemical roguing technology for breeding hybrid rice according to claim 1 is characterized in that anti-herbicide gene adopts optionally non-broad-spectrum herbicide gene.
3, chemical roguing technology for breeding hybrid rice according to claim 2 is characterized in that non-wide spectrum anti-herbicide gene is the bar gene.
4, chemical roguing technology for breeding hybrid rice according to claim 2 is characterized in that non-wide spectrum anti-herbicide gene is the aroA gene.
5,, it is characterized in that transgenic maintainer line is three is the transgenosis stable strain of the maintenance line of male sterile line according to claim 1 or 2 or 3 or 4 described chemical roguing technology for breeding hybrid rice.
6,, it is characterized in that adopting particle bombardment or agriculture bacillus mediated conversion method or electrization or its conversion method or ultrasonic conversion method or conventional breeding hybridization transformation method anti-herbicide gene to be imported the maintenance line of ternary hybrid rice according to claim 1 or 2 or 3 or 4 described chemical roguing technology for breeding hybrid rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99115125 CN1247699A (en) | 1999-09-10 | 1999-09-10 | Chemical roguing technology for breeding hybrid rice |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99115125 CN1247699A (en) | 1999-09-10 | 1999-09-10 | Chemical roguing technology for breeding hybrid rice |
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| CN1247699A true CN1247699A (en) | 2000-03-22 |
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| CN 99115125 Pending CN1247699A (en) | 1999-09-10 | 1999-09-10 | Chemical roguing technology for breeding hybrid rice |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160529A (en) * | 2013-03-18 | 2013-06-19 | 中国农业科学院生物技术研究所 | Method for cultivating transgenosis hybrid rice |
| CN106538377A (en) * | 2016-11-08 | 2017-03-29 | 上海市农业科学院 | The method of sterile line purity and its application in a kind of raising ternary hybrid rice |
| CN110291986A (en) * | 2017-09-28 | 2019-10-01 | 彭基明 | Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
| AU2014277085B2 (en) * | 2013-06-04 | 2020-06-25 | Basf Se | Improved hybrid seed production method |
-
1999
- 1999-09-10 CN CN 99115125 patent/CN1247699A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160529A (en) * | 2013-03-18 | 2013-06-19 | 中国农业科学院生物技术研究所 | Method for cultivating transgenosis hybrid rice |
| AU2014277085B2 (en) * | 2013-06-04 | 2020-06-25 | Basf Se | Improved hybrid seed production method |
| CN106538377A (en) * | 2016-11-08 | 2017-03-29 | 上海市农业科学院 | The method of sterile line purity and its application in a kind of raising ternary hybrid rice |
| CN110291986A (en) * | 2017-09-28 | 2019-10-01 | 彭基明 | Rice paddy seed product containing anti-herbicide gene of field planting and preparation method thereof |
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