CN106342623B - It is a kind of to infect method with the efficient symbiosis of raspberry exotrophic mycorrhiza - Google Patents
It is a kind of to infect method with the efficient symbiosis of raspberry exotrophic mycorrhiza Download PDFInfo
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- CN106342623B CN106342623B CN201610702350.1A CN201610702350A CN106342623B CN 106342623 B CN106342623 B CN 106342623B CN 201610702350 A CN201610702350 A CN 201610702350A CN 106342623 B CN106342623 B CN 106342623B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
- C05F11/04—Horticultural earth from peat
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- Engineering & Computer Science (AREA)
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- Organic Chemistry (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Method is infected with the efficient symbiosis of raspberry exotrophic mycorrhiza the invention discloses a kind of, comprising the following steps: Tree Species Selection, mycorhiza infect matrix preparation, infect seedling pre-treatment, infect method optimization, site preparation, transplanting, field management.Method of the invention is easy to operate, easy to spread, and transplants success rate up to 90~95%, improves 5~10% than conventional method;Rapidly, height of seedling increases by 20~30cm than conventional method up to 50~80cm after sowing 1 year for growth;Use edible fungus bran, coco bran, peat soil as infecting matrix in the present invention, production cost can be made to reduce by 20%, survival rate is planted up to 95% or more, 5~10% are improved than conventional method, the forest resistance after infecting using self-control purple Lasiosphaera fenzlii liquid spawn, which improves 10~20%, yield than conventional method, can be improved 5~10%.
Description
Technical field
Method is infected with the efficient symbiosis of raspberry exotrophic mycorrhiza the present invention relates to a kind of, belongs to mycorhiza and infects technical field.
Background technique
Raspberry (Kdaeobatus) is rosaceae (Rosaceae) rubus (Rubus) plant, also known as rasp berry, for for many years
Raw shrub species, the gorgeous fruit beauty of raspberry flower.It can beautify the environment, conserve water and soil, there is high economy, ecology and social benefit.Tree
The tender succulence of certain kind of berries berry, unique flavor have very high value of exploiting and utilizing.Raspberry contains 15-17 kinds of amino acid, wherein eight
Kind it is necessary to human body, containing sugar 3.6% one 13.4%, containing soluble solid 7.0% one 14.3%, 1.2%- containing organic acid
5.4%, 0.3%-0.6% containing pectin, 1.0%-2.7 containing protein are described as " king of fruit " in the world.Raspberry is in the U.S. and east
Europe regional culture is more.China raspberry is introduced by Russia countrymen residing abroad earliest, and the past is mostly wild state.In addition, Rubus corchorifolius contain it is special
Fragrance has 51 kinds of volatile ingredient, mainly there is γ-firpene, nopinene, p- methyl acetophenone, laurene, limonene, cymene
Deng.For traditional Chinese medicine, raspberry root, stem, leaf, fruit can be used as medicine, root nature and flavor slight bitter, pungent, flat, have dispelling wind and eliminating dampness, work
The effect of blood silt, removing toxic substances sore, cures mainly lumbago due to wind-wetness evil, dysentery, spermatorrhea, venomous snake bite, amenorrhoea dysmenorrhea, eczema, infantile malnutrition
The common medicine of equal diseases and Miao ethnic group's medicine.
In recent years, it is the area for expanding cultivar, improves the yield and quality, meet people to the need of nutrition and health care fruit
It asks, various regions introduce some variety sources from foreign countries successively, directly utilize in production, but since implantation methods are all with routine
Implantation methods, because planting, improper, density is unreasonable, fertility not enough results in pest and disease damage sprawling, while being grown in more and being mixed with raspberry
The mountain area or the foot of a mountain hillside fields of gravel, by the foot of a mountain area severe water and soil erosion, causing current extensive raspberry to plant, survival rate is low, produces
Amount decline, hinders the development of raspberry industry to a certain extent.
Summary of the invention
The defects of present invention is in order to overcome existing raspberry survival rate, yield decline, quality to reduce provides a kind of high with raspberry
The exotrophic mycorrhiza of effect symbiosis infects method, and this method is technically simple, easy to spread, can make the growth and the hair that rapidly promote raspberry
It educates, improves height of seedling, ground diameter, lateral root number and the dry weight of raspberry, especially significantly mentioned on nutrien utilization, resistance, quality and yield
It is high.
In order to solve the above-mentioned technical problem, the present invention adopts the following technical scheme that: a kind of outer with the efficient symbiosis of raspberry
Raw mycorhiza infects method, comprising the following steps:
(1) Tree Species Selection: selection raspberry kind best in quality and high yield;
(2) mycorhiza infects matrix preparation: in the 240m of covering sunshade net2In standard greenhouse, divide calculating by weight, by mud
2~4 parts of charcoal soil, 3~4 parts of coco bran, 3~5 parts of edible fungus bran, 2~3 parts of chicken manure and 0.05 part of purple Lasiosphaera nipponica(Kawam.)Y.Kobayasi liquid spawn are mixed
Even, pH is controlled 5.5~7.0, and it is spare to be made into wide 80~100cm, high 20~50cm nursery high bed;The purple Lasiosphaera fenzlii bacterium solution
Body strain is purple Lasiosphaera nipponica(Kawam.)Y.Kobayasi liquid spawn disclosed in the patent of invention of applicant's earlier application, the title of the patent of invention
Are as follows: a kind of production method, the patent No. of purple Lasiosphaera fenzlii liquid spawn: ZL201410082599.8;
(3) infect seedling pre-treatment: acquiring 1 year raspberry seedling before Yu Chunji rudiment, height of seedling 10cm or more, ground diameter 0.3cm with
On;Raspberry seedling impregnates 5~10min with the antiseptic solution that NaClO is prepared into 2 ‰ before infecting and makees disinfection pretreatment;
(4) method optimization is infected: through the segment of step (3) treated raspberry seedling is cut into 5~8cm it will immerse 0.8~
The heteroauxin (IAA) of 1.2mg/L with zeatin mixed solution 30 seconds, then will set by 8~15cm of spacing in the rows, 15~20cm of line-spacing
Certain kind of berries branch is gently inserted into 2~3cm of matrix, is watered with root water, while cysteine is added into culture medium, and added cysteine adds
Dosage is 200~500mg/L;Temperature will be controlled at 20~27 DEG C in standard greenhouse, relative humidity control is 55~70%, light
According to for 1500lx, daily illumination 12h;This control condition can make raspberry seedling be in the state of excitation a kind of, purple Lasiosphaera fenzlii bacterium solution
It is easier to be adhered to seedling surface and optimize into cell, and cultivate 25 days with this condition, be managed collectively;
(5) site preparation: carrying out drawing block site preparation according to natural slope, and the slope shape leveling on ground is whole, opens up wasteland in two times: just
It cultivates, at least 0.50 meter of depth, it is preferred to use excavator is just cultivated;Reclaim: ground grading digs base fertilizer ditch, after 0.30 meter of depth or more,
Each standard greenhouse applies thin night soil, urea or potassium dihydrogen phosphate 20kg;
(6) it transplants: after 1 year when Miao Changzhi 50cm or more, exotrophic mycorrhiza can be infected to seedling band soil at the cloudy day in 2~March
Lifting, then in field cultivating;
(7) field management: after A, 30 days, sunshade net being opened, and makes greenhouse vented exhaust;B, it waters: preceding 3 days slotting early periods
Watering is primary, it is preceding insert take root after watering in 5 days it is primary;C, apply fertilizer: it is preceding insert 40 days after, 3kg compound fertilizer is dissolved in 100kg water, spray
It spills around the seedling in standard greenhouse;D, weeding: according to the growth cycle of raspberry, weeding is periodically carried out.
Compared with the prior art, the advantages of the present invention are as follows: method of the invention is easy to operate, easy to spread, Er Qieyi
Success rate is planted up to 90~95%, improves 5~10% than conventional method;Growth rapidly, after sowing 1 year height of seedling up to 50~
80cm increases by 20~30cm than conventional method;Use edible fungus bran, coco bran, peat soil as matrix is infected in the present invention, it can
Production cost is set to reduce by 20%, plantation survival rate improves 5~10% up to 95% or more, than conventional method, uses self-control purple
Forest resistance after Lasiosphaera fenzlii liquid spawn is infected can be improved 5~10% than conventional method raising 10~20%, yield.Therefore, originally
The method of invention can realize the scale of raspberry root nursery stock, industrialization production, be particluarly suitable for Karst region Efficient Cultivation.
Specific embodiment
In order to keep the object of the invention, technical solution and advantage clearer, the present invention is made into one below with reference to embodiment
The detailed description of step.
Embodiment 1
A kind of exotrophic mycorrhiza of the efficient symbiosis of and raspberry of the invention infects method, comprising the following steps:
(1) Tree Species Selection: selection raspberry kind best in quality and high yield " U.S. 22 " (Royallty);
(2) mycorhiza infects matrix preparation: in the 240m of covering sunshade net2In standard greenhouse, divide calculating by weight, by mud
2 parts of charcoal soil, 3 parts of coco bran, 3 parts of edible fungus bran, 2 parts of chicken manure and 0.05 part of purple Lasiosphaera nipponica(Kawam.)Y.Kobayasi liquid spawn mixing, pH control exist
5.5, it is spare to be made into wide 80cm, high 20cm nursery high bed;
(3) infect seedling pre-treatment: acquiring 1 year raspberry seedling before Yu Chunji rudiment, height of seedling 10cm or more, ground diameter 0.3cm with
On;Raspberry seedling impregnates 5min with the antiseptic solution that NaClO is prepared into 2 ‰ before infecting and makees disinfection pretreatment;
(4) it infects method optimization: will immerse 0.8mg/L's through the segment of step (3) treated raspberry seedling is cut into 5cm
Heteroauxin (IAA) with zeatin mixed solution 30 seconds, raspberry branch is gently then inserted into base by spacing in the rows 8cm, line-spacing 15cm
Matter 2cm is watered with root water, while the cysteine of 200mg/L is added into culture medium;Temperature will be controlled in standard greenhouse
20~27 DEG C, relative humidity control is 55~70%, illumination 1500lx, daily illumination 12h, this control condition can make raspberry
Seedling is in a kind of state of excitation, and purple Lasiosphaera fenzlii bacterium solution is easier to be adhered to seedling surface and optimize into cell, and herein
Under the conditions of cultivate 25 days, be managed collectively;
(5) site preparation: carrying out drawing block site preparation according to natural slope, and the slope shape leveling on ground is whole, opens up wasteland in two times: just
It cultivates, at least 0.50 meter of depth, it is preferred to use excavator is just cultivated;Reclaim: ground grading digs base fertilizer ditch, after 0.30 meter of depth or more,
Each standard greenhouse applies thin night soil 20kg;
(6) it transplants: after 1 year when Miao Changzhi 50cm or more, exotrophic mycorrhiza can be infected to seedling band soil 2 months cloudy days and risen
Seedling, then in field cultivating;
(7) field management: after A, 30 days, sunshade net being opened, and makes greenhouse vented exhaust;B, it waters: preceding 3 days slotting early periods
Watering is primary, it is preceding insert take root after watering in 5 days it is primary;C, apply fertilizer: it is preceding insert 40 days after, 3kg compound fertilizer is dissolved in 100kg water, spray
It spills around the seedling in standard greenhouse;D, weeding: according to the growth cycle of raspberry, weeding is periodically carried out.
Method of the invention is easy to operate, easy to spread, and transplants success rate up to 90~95%, mentions than conventional method
It is high by 5~10%;Rapidly, height of seedling increases by 20~30cm than conventional method up to 50~80cm after sowing 1 year for growth;In the present invention
Use edible fungus bran, coco bran, peat soil as matrix is infected, production cost can be made to reduce by 20%, plantation survival rate is reachable
95% or more, 5~10% are improved than conventional method, the forest resistance after infecting using self-control purple Lasiosphaera fenzlii liquid spawn is than tradition
Method, which improves 10~20%, yield, can be improved 5~10%.Therefore, method of the invention can realize the scale of raspberry root nursery stock
Change, industrialization production, is particluarly suitable for Karst region Efficient Cultivation.
Embodiment 2
A kind of exotrophic mycorrhiza of the efficient symbiosis of and raspberry of the invention infects method, comprising the following steps:
(1) Tree Species Selection: selection raspberry kind best in quality and high yield " U.S. 22 " (Royallty);
(2) mycorhiza infects matrix preparation: in the 240m of covering sunshade net2In standard greenhouse, divide calculating by weight, by mud
3 parts of charcoal soil, 3.5 parts of coco bran, 4 parts of edible fungus bran, 2.5 parts of chicken manure and purple Lasiosphaera nipponica(Kawam.)Y.Kobayasi liquid spawn 20g are mixed, and pH control exists
6.0, it is spare to be made into wide 90cm, high 30cm nursery high bed;
(3) infect seedling pre-treatment: acquiring 1 year raspberry seedling before Yu Chunji rudiment, height of seedling 10cm or more, ground diameter 0.3cm with
On;Raspberry seedling impregnates 8min with the antiseptic solution that NaClO is prepared into 2 ‰ before infecting and makees disinfection pretreatment;
(4) it infects method optimization: will immerse 1.2mg/L's through the segment of step (3) treated raspberry seedling is cut into 6cm
Heteroauxin (IAA) with zeatin mixed solution 30 seconds, raspberry branch is gently then inserted into base by spacing in the rows 12cm, line-spacing 18cm
Matter 3cm is watered with root water, while the cysteine of 250mg/L is added into culture medium;Temperature is controlled 23 in standard greenhouse
DEG C, relative humidity control is 60%, illumination 1500lx, daily illumination 12h, this control condition can make raspberry seedling be in one kind
The state of excitation, purple Lasiosphaera fenzlii bacterium solution is easier to be adhered to seedling surface and optimize into cell, and cultivates with this condition
It 25 days, is managed collectively;
(5) site preparation: carrying out drawing block site preparation according to natural slope, and the slope shape leveling on ground is whole, opens up wasteland in two times: just
It cultivates, at least 0.50 meter of depth, it is preferred to use excavator is just cultivated;Reclaim: ground grading digs base fertilizer ditch, after 0.30 meter of depth or more,
Each standard greenhouse applies potassium dihydrogen phosphate 20kg;
(6) it transplants: after 1 year when Miao Changzhi 50cm or more, exotrophic mycorrhiza can be infected to seedling band soil at the cloudy day in 2~March
Lifting, then in field cultivating;
(7) field management: after A, 30 days, sunshade net being opened, and makes greenhouse vented exhaust;B, it waters: preceding 3 days slotting early periods
Watering is primary, it is preceding insert take root after watering in 5 days it is primary;C, apply fertilizer: it is preceding insert 40 days after, 3kg compound fertilizer is dissolved in 100kg water, spray
It spills around the seedling in standard greenhouse;D, weeding: according to the growth cycle of raspberry, weeding is periodically carried out.
Method of the invention is easy to operate, easy to spread, and transplants success rate up to 90~95%, mentions than conventional method
It is high by 5~10%;Rapidly, height of seedling increases by 20~30cm than conventional method up to 50~80cm after sowing 1 year for growth;In the present invention
Use edible fungus bran, coco bran, peat soil as matrix is infected, production cost can be made to reduce by 20%, plantation survival rate is reachable
95% or more, 5~10% are improved than conventional method, the forest resistance after infecting using self-control purple Lasiosphaera fenzlii liquid spawn is than tradition
Method, which improves 10~20%, yield, can be improved 5~10%.Therefore, method of the invention can realize the scale of raspberry root nursery stock
Change, industrialization production, is particluarly suitable for Karst region Efficient Cultivation.
Claims (2)
1. a kind of infect method with the efficient symbiosis of raspberry exotrophic mycorrhiza, it is characterised in that the following steps are included:
(1) Tree Species Selection: selection raspberry kind best in quality and high yield;
(2) mycorhiza infects matrix preparation: in the 240m of covering sunshade net2In standard greenhouse, divide calculating by weight, by peat soil 2
~4 parts, 3~4 parts of coco bran, 3~5 parts of edible fungus bran, 2~3 parts of chicken manure and 0.05 part of purple Lasiosphaera nipponica(Kawam.)Y.Kobayasi liquid spawn mixing, pH
Then it is spare to be made into wide 80~100cm, high 20~50cm nursery high bed 5.5~7.0 for control;
(3) it infects seedling pre-treatment: acquiring 1 year raspberry seedling, height of seedling 10cm or more, ground diameter 0.3cm or more before Yu Chunji rudiment;Tree
Certain kind of berries seedling impregnates 5~10min with the antiseptic solution that NaClO is prepared into 2 ‰ before infecting and makees disinfection pretreatment;
(4) it infects method optimization: 0.8~1.2mg/ will be immersed through the segment of step (3) treated raspberry seedling is cut into 5~8cm
The heteroauxin of L with zeatin mixed solution 30 seconds, then by 8~15cm of spacing in the rows, 15~20cm of line-spacing by raspberry branch gently
It is inserted into 2~3cm of matrix, is watered with root water, while adding cysteine into culture medium;Temperature will be controlled in standard greenhouse
20~27 DEG C, relative humidity control is 55~70%, illumination 1500lx, daily illumination 12h, and cultivates 25 with this condition
It, is managed collectively;
(5) site preparation: carrying out drawing block site preparation according to natural slope, and the slope shape leveling on ground is whole, opens up wasteland: just cultivating in two times,
At least 0.50 meter of depth, it is preferred to use excavator is just cultivated;Reclaim: ground grading digs base fertilizer ditch, after 0.30 meter of depth or more, often
A standard greenhouse applies thin night soil, urea or potassium dihydrogen phosphate 20kg;
(6) it transplants: after 1 year when Miao Changzhi 50cm or more, exotrophic mycorrhiza can be infected to seedling band soil at the cloudy day in 2~March and risen
Seedling, then in field cultivating;
(7) field management: after A, 30 days, sunshade net being opened, and makes standard greenhouse vented exhaust;B, it waters: preceding 3 days slotting early periods
Watering is primary, it is preceding insert take root after watering in 5 days it is primary;C, apply fertilizer: it is preceding insert 40 days after, 3kg compound fertilizer is dissolved in 100kg water, spray
It spills around the seedling in standard greenhouse;D, weeding: according to the growth cycle of raspberry, weeding is periodically carried out.
2. according to claim 1 infect method with the efficient symbiosis of raspberry exotrophic mycorrhiza, it is characterised in that: step (4)
Described in cysteine additive amount be 200~500mg/L.
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CN108191566A (en) * | 2018-01-19 | 2018-06-22 | 贵州省生物研究所 | A kind of gold Rosa roxburghii Tratt orchard soil improvement bio-fertilizer and soil improvement method |
CN110896768A (en) * | 2019-10-29 | 2020-03-24 | 南京林业大学 | Method for cultivating root-rich rootstock seedlings of apocarya |
CN113207552A (en) * | 2021-05-14 | 2021-08-06 | 山东省林业科学研究院 | Seedling mycorrhization method taking mycorrhizal fungi hypha culture medium as seedling culture medium and application of seedling mycorrhization method |
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CN101323549A (en) * | 2008-07-18 | 2008-12-17 | 东北师范大学 | Sustained and controlled release type organic inorganic block fertilizer for root and preparation and use thereof |
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