CN106134740A - The method that application melatonin improves alfalfa alumite ability - Google Patents

The method that application melatonin improves alfalfa alumite ability Download PDF

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CN106134740A
CN106134740A CN201610532724.XA CN201610532724A CN106134740A CN 106134740 A CN106134740 A CN 106134740A CN 201610532724 A CN201610532724 A CN 201610532724A CN 106134740 A CN106134740 A CN 106134740A
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alfalfa
melatonin
soil
seed
alumite
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CN106134740B (en
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胡龙兴
张强
向佐湘
刘明稀
曾宁波
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Hunan Agricultural University
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Hunan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of method applying melatonin to improve alfalfa alumite ability.The method includes: 1. by Alfalfa Growing in soil, and carries out the irrigation and drainage of moisture according to weather, impose seed manure and middle weeding;2. melatonin and potassium dihydrogen phosphate are mixed into aqueous products, wherein melatonin 150 μm l/L, potassium dihydrogen phosphate 0.35%;3. during alfalfa plant height 10cm, spraying described aqueous products in alfalfa blade face, spraying frequency is to spray 1 time for 10 days, sprays altogether 2 times, and the described aqueous products amount of application of each every mu is 45~55L.In the present invention, melatonin is a kind of amine bormones, nontoxic to plant and animal, and foliage-spray consumption is few, instant effect, workable, the growth of Herba Medicaginis under acid-aluminum stress can be remarkably promoted, increase chlorophyll content, improve Alfalfa Output and quality.

Description

The method that application melatonin improves alfalfa alumite ability
Technical field
The present invention relates to grass industry and management, particularly relate to a kind of application melatonin and improve alfalfa alumite ability Method.
Background technology
Acid ground refers to that pH value is less than the soil of 7.0, and for agricultural production, topsoil pH value is less than 6.6 are acid ground.Acid ground account for the whole world can farming soil 40%, account for the 21% of land area in China, extensively It is distributed in Red-yellow, south, and along with the deterioration of environment, the area of acid ground is being constantly increasing.Aluminum is content in the earth's crust The abundantest metallic element, accounts for about the 7% of earth crustal mass.Under field conditions (factors), it mainly exists with aluminosilicate form, raw Thing utilizability is the least.Soil acidification is made in recent years due to use and the Acid Rain Effect of agrochemical, a large amount of in acid ground The aluminum of indissoluble state, with ionic state dissolution, presents activated state, and soluble aluminum concentration rises, and destroys the physiology in crops body Biochemical process, it is suppressed that the growth of crop, reduces crop yield.Therefore, the crop aluminum poison that soil acidification causes has become complete The focus that ball biology and environmental science are paid close attention to.
Melatonin is the neuro-endocrinology hormone of a kind of little molecule finding in animal body and being widely present the earliest, is one Planting indoles tryptamines compound, chemical name is melatonin.Participate in vertebrate physiological regulation, including Circadian rhythm and photoperiodical reaction.In animal field, melatonin is not only a kind of hormone, it is also possible to directly remove multiple not similar shape The free radical of state, has raising immunity of organisms, the effect of slow down aging.
Alfalfa is a kind of perennial high quality forage of pulse family Medicago, well developed root system, and main root is thick, has stronger resistance to Cold, drought resisting and regeneration capacity, be of high nutritive value, and good palatability, various domestic animals the most relatively eating, is described as " King of Pasture ".According to Measure, containing thick protein 15-25, lysine content 1.05-1.38% in alfalfa dry, higher 1-than Semen Maydis respectively 1.5 times, 4-5 times.Alfalfa is the crop of improvement soil, is also to maintain the important plant of water and soil.The root system of alfalfa Burying deep and broad, its root nodule bacteria can fix the nitrogen in air, dramatically increases soil nitrogenous amount.Moreover it is possible to stay big in soil Amount root stubble, increases a large amount of organic for soil, and Soil building improves the yield of succession crop.Alfalfa has the longest flower Phase, is also a kind of important nectariferous plant.
Due to the development of domestic animal husbandry, the cultivated area of China Herba Medicaginis constantly expands in recent years, but is concentrated mainly on north Area, side, and southern area cultivated area is relatively fewer.Trace it to its cause, more sensitive to acid mainly due to alfalfa, at pH be Conveniently, when soil pH is less than 6.0, the growing way of alfalfa is affected, and causes soil-grown between 6.5-7.5 A large amount of root nodule bacteria can not normally breed and survive.When soil pH is less than 4.5, alfalfa normal growth is heavily suppressed, Even result in plant dead, therefore, it is difficult to spread plantation on acid ground.Recently as southern area animal husbandry Fast development, the especially fast development of dairy, the demand of high protein high quality forage alfalfa is also gradually increased, but Herba Medicaginis is sensitive to acid ground, has had a strong impact on the alfalfa plantation in southern area.The relatively low pH level of acid ground and The impact on Herba Medicaginis of the higher activated aluminum, initial reaction is to inhibit sprouting and the growth promoter of seedling of alfalfa seed, and then Suppression Herba Medicaginis root system growth, and thus suppression moisture, the absorption of nutrient, long acid-aluminum stress can cause overground part mitogenetic Length is obstructed.
Cycle required for using the alumite ability of breeding technique raising Herba Medicaginis is long, is difficult in adapt to Herba Medicaginis in a short time in south Side's acid ground popularizing planting and the needs of animal husbandry development.
Summary of the invention
The purpose of the present invention is that the impact grown Growth of Alfalfa for acid-aluminum stress in acid soil in the south, A kind of method applying melatonin to improve alfalfa alumite ability is provided.
For reaching above-mentioned purpose, the technical solution used in the present invention is: a kind of application melatonin improves alfalfa alumite The method of ability, comprises the following steps:
1. by Alfalfa Growing in soil, and carry out the irrigation and drainage of moisture according to weather, impose seed manure and intertillage removes Grass;
2. melatonin and potassium dihydrogen phosphate are mixed into aqueous products, wherein melatonin 150 μm l/L, potassium dihydrogen phosphate 0.35%;
3. during alfalfa plant height 10cm, spraying described aqueous products in alfalfa blade face, spraying frequency is 10 It spray 1 time, sprays 2 times altogether, and the described aqueous products amount of application of each every mu is 45~55L.
The plantation of described alfalfa includes:
A, at ambient temperature, is soaked in alfalfa seed in the potassium permanganate solution that mass concentration is 0.15% and disappears Poison 5min, dries after then seed being removed filter dry moisture;
B, soil is ploughed, smooth and basal dressing, then seed bar is sowed at smooth after level ground bed on;
C, holding Soil of Lawn Seed bed moisten and promote sub-sprouting, carry out the irrigation and drainage of moisture according to weather, impose seed manure and intertillage Weeding.
Described step B is: by soil deep tillage, applies calcium superphosphate 35kg/ mu and ammonium hydrogen carbonate 45kg/ mu as base manure, Then with machinery rotary tillage, and with mass concentration be 3% Furadan 3kg/ mu, mass concentration be 75% fenaminosulf wettable powder 1.5kg/ even spreading fertilizer over the fields in soil per mu carries out disinfection, and then sprays preemergence herbicide by smooth for ground simultaneously, broadcasts to JIUYUE in autumn Plant alfalfa seed, by the amount drilling of 1~1.5kg/ mu, after planting cover fine earth about 0.5cm.
After emerging two weeks in described step C, in conjunction with in weeding impose a seed manure.
Although being found that the existence of melatonin in plant very early, simply its content is relatively low, for the level of ng/g, the most not Cause the attention of people;But it is found that in some medicinal plants, the content of melatonin is the highest, melatonin content in its seed It is even up to the level of μ g/g.Showing according further to research, melatonin can promote growth and the generation of side root of plant, carry The resistance of high plant (low temperature, saline and alkaline and water stress etc.).Still more, melatonin is originally as endogenous amine bormones, to planting Thing and the nontoxic noresidue of animal, can be widely applied to agricultural production.Based on this, the present invention is creatively raw at alfalfa Applying in growth process with melatonin is main active ingredient, is aided with the product of potassium dihydrogen phosphate, finds the present invention and prior art Compare, achieve following good effect:
1. melatonin is a kind of amine bormones, nontoxic to plant and animal;
2. foliage-spray consumption is few, instant effect, workable;
3. can remarkably promote the growth of alfalfa under acid-aluminum stress, increase its chlorophyll content, improve alfalfa thick Albumen and
Crude fat content, thus improve Alfalfa Output and quality;
4. alfalfa problem of retarded growth in acid ground can effectively be solved, for alfalfa in south acid Property soil
In popularizing planting provide technical support.
Accompanying drawing explanation
Fig. 1 be melatonin under acid-aluminum stress alfalfa plant height affect coordinate diagram.
Fig. 2 be melatonin under acid-aluminum stress alfalfa chlorophyll content affect coordinate diagram.
Detailed description of the invention
Describe in detail as follows below in conjunction with embodiment and accompanying drawing:
One, the structural formula of melatonin
The structural formula of melatonin is as shown in Equation 1:
Two, embodiment
1, the plantation of alfalfa
A, to alfalfa seed sterilize
' huge energy 995 ' seed is material, at ambient temperature, is soaked by alfalfa seed to select commercial alfalfa cultivars Steep sterilization 5min in the beaker equipped with 0.15% (quality) potassium permanganate solution, then seed is removed filter dry;
B, sowing
Being experimental field yellow earth, pH value is 5.5~6.0.Before Bo Zhong, ground is ploughed deeply, apply calcium superphosphate 35kg/ mu and carbonic acid Hydrogen ammonium 45kg/ mu is as base manure, then with machinery rotary tillage, and with 3% Furadan 3kg/ mu, 75% fenaminosulf wettable powder 1.5kg/ even spreading fertilizer over the fields in soil per mu carries out disinfection, and then sprays preemergence herbicide by smooth for ground simultaneously;JIUYUE is broadcast in the fall Kind, by the amount drilling of 1~1.5kg/ mu, after planting cover fine earth about 0.5cm;
C, seedling management
Keep Soil of Lawn Seed bed to moisten and promote sub-sprouting, after seed sprouting, rationally carry out the irrigation and drainage of moisture according to weather condition, And the combinations in about two weeks after emerging impose a seed manure during weeding;
2, the using of melatonin solution
When seedling of alfalfa plant height about about 10cm, select fine day the morning, use containing 150 μm l/L melatonin and The potassium dihydrogen phosphate mixed aqueous solution of 0.35% carries out foliage-spray, and every mu sprays mixed liquor 45~55L, and spraying frequency is 10 days Spray 1 time, spray 2 times altogether;Set up a matched group simultaneously, spray same amount of tap water;Foliage-spray solution is adopted after processing 7 days Use five point samplings, carry out the mensuration of plant height, chlorophyll content, crude protein and crude fat.
According to above-mentioned steps, melatonin water solution mixture is processed the result obtained with matched group and is listed in table 1 and figure 1, in 2:
The impact on Herba Medicaginis nutritional quality of table 1 melatonin
Result shows: as depicted in figs. 1 and 2, under the conditions of the acid ground that pH is 5.5~about 6.0, alfalfa Growth is affected, and after foliage-spray melatonin solution, promotes the growth of alfalfa, improves chlorophyll content, from And improve alumite ability and the adaptability of alfalfa;In addition, under the conditions of acid ground, foliage-spray melatonin Solution, adds crude protein and crude fat content in blade, improves the nutritional quality of Herba Medicaginis.

Claims (4)

1. apply the method that melatonin improves alfalfa alumite ability for one kind, it is characterised in that comprise the following steps:
1. by Alfalfa Growing in soil, and carry out the irrigation and drainage of moisture according to weather, impose seed manure and middle weeding;
2. melatonin and potassium dihydrogen phosphate are mixed into aqueous products, wherein melatonin 150 μm l/L, potassium dihydrogen phosphate 0.35%;
3. during alfalfa plant height 10cm, spraying described aqueous products in alfalfa blade face, spraying frequency is spray 1 in 10 days Secondary, spray altogether 2 times, the described aqueous products amount of application of each every mu is 45~55L.
Application melatonin the most according to claim 1 improves the method for alfalfa alumite ability, it is characterised in that institute The plantation stating alfalfa includes:
A, at ambient temperature, is soaked in the potassium permanganate solution that mass concentration is 0.15% sterilization by alfalfa seed 5min, dries after then seed being removed filter dry moisture;
B, soil is ploughed, smooth and basal dressing, then seed bar is sowed at smooth after level ground bed on;
C, holding Soil of Lawn Seed bed moisten and promote sub-sprouting, carry out the irrigation and drainage of moisture according to weather, impose seed manure and middle weeding.
Application melatonin the most according to claim 2 improves the method for alfalfa alumite ability, it is characterised in that institute Stating step B is: by soil deep tillage, applies calcium superphosphate 35kg/ mu and ammonium hydrogen carbonate 45kg/ mu as base manure, then with machinery Rotary tillage, and with mass concentration be 3% Furadan 3kg/ mu, mass concentration be that 75% fenaminosulf wettable powder 1.5kg/ is per mu even Spread fertilizer over the fields in soil and carry out disinfection, then spray preemergence herbicide by smooth for ground simultaneously, to JIUYUE sowing in autumn alfalfa kind Son, by the amount drilling of 1~1.5kg/ mu, after planting covers fine earth about 0.5cm.
Application melatonin the most according to claim 2 improves the method for alfalfa alumite ability, it is characterised in that institute State two weeks in step C after emerging, in conjunction with in weeding impose a seed manure.
CN201610532724.XA 2016-07-07 2016-07-07 Method for improving acid-aluminum resistance of alfalfa in acid soil by applying melatonin Expired - Fee Related CN106134740B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108713368A (en) * 2018-05-29 2018-10-30 湖南农业大学 A method of promoting the lower alfalfa seed sprouting of sour copper stress and health of root growth
CN113016389A (en) * 2021-03-18 2021-06-25 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN115413536A (en) * 2022-09-27 2022-12-02 河南科技大学 Method for improving quality of selfheal medicinal material by spraying melatonin
CN116836814A (en) * 2023-06-28 2023-10-03 贵州省草业研究所 Endophytic fungus and application thereof in improving aluminum tolerance of alfalfa

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102731205A (en) * 2012-06-14 2012-10-17 上海交通大学 Foliar fertilizer promoting alfalfa seedling growth in acidic soil

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102731205A (en) * 2012-06-14 2012-10-17 上海交通大学 Foliar fertilizer promoting alfalfa seedling growth in acidic soil

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HAITAO SHI, ETC.: "Comparative physiological, metabolomic, and transcriptomic analyses reveal echanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by exogenous melatonin", 《JOURNAL OF EXPERIMENTAL BOTANY》 *
HARDELAND, R: "Melatonin in Plants - Diversity of Levels and Multiplicity of Functions", 《FRONTIERS IN PLANT SCIENCE》 *
姜超强 等: "褪黑素与植物抗逆性研究进展", 《生物技术通报》 *
赵鑫 等: "荞麦对干旱及酸铝胁迫的响应", 《山西农业大学(自然科学版)》 *
邱晓: "铝胁迫对紫花苜蓿的影响及外源有机酸的缓解机制", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
顾丽慧: "优质高产紫花苜蓿的栽培技术", 《畜牧兽医杂志》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108713368A (en) * 2018-05-29 2018-10-30 湖南农业大学 A method of promoting the lower alfalfa seed sprouting of sour copper stress and health of root growth
CN108713368B (en) * 2018-05-29 2021-03-02 湖南农业大学 Method for promoting germination of alfalfa seeds and healthy growth of root systems under acid copper stress
CN113016389A (en) * 2021-03-18 2021-06-25 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN113016389B (en) * 2021-03-18 2022-05-20 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN115413536A (en) * 2022-09-27 2022-12-02 河南科技大学 Method for improving quality of selfheal medicinal material by spraying melatonin
CN115413536B (en) * 2022-09-27 2024-04-05 河南科技大学 Method for improving quality of selfheal medicinal material by melatonin spraying
CN116836814A (en) * 2023-06-28 2023-10-03 贵州省草业研究所 Endophytic fungus and application thereof in improving aluminum tolerance of alfalfa
CN116836814B (en) * 2023-06-28 2024-01-30 贵州省草业研究所 Endophytic fungus and application thereof in improving aluminum tolerance of alfalfa

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