CN115413536A - Method for improving quality of selfheal medicinal material by spraying melatonin - Google Patents
Method for improving quality of selfheal medicinal material by spraying melatonin Download PDFInfo
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- CN115413536A CN115413536A CN202211183281.XA CN202211183281A CN115413536A CN 115413536 A CN115413536 A CN 115413536A CN 202211183281 A CN202211183281 A CN 202211183281A CN 115413536 A CN115413536 A CN 115413536A
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- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 title claims abstract description 120
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 title claims abstract description 120
- 229960003987 melatonin Drugs 0.000 title claims abstract description 120
- 238000005507 spraying Methods 0.000 title claims abstract description 58
- 235000008113 selfheal Nutrition 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000000463 material Substances 0.000 title claims abstract description 24
- 244000299788 selfheal Species 0.000 title 1
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 15
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 10
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Ecology (AREA)
- Pest Control & Pesticides (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A method for improving the quality of selfheal medicinal materials by spraying melatonin comprises the following steps: spraying a melatonin solution of 50-200 mu mol/L to the selfheal plants during the flowering period of the selfheal plants. According to the invention, melatonin with a specific concentration is sprayed to the selfheal during the flowering period of selfheal plants, and the antioxidation performance, the photosynthetic capacity and the secondary metabolic capability of the selfheal during the flowering period are improved by using the melatonin, so that the content of medicinal components in selfheal clusters can be effectively increased, and the quality of medicinal materials is improved.
Description
Technical Field
The invention belongs to the technical field of Chinese herbal medicine planting, and particularly relates to a method for improving the quality of selfheal medicinal materials by spraying melatonin.
Background
Selfheal, a perennial herb of the family labiatae, has important medicinal value in dried fruit clusters. The prunella spike contains various active ingredients such as triterpenes, steroids, flavonoids, phenolic acids and the like, and has the activities of resisting bacteria, viruses, inflammation, oxidation, tumors, blood sugar and the like, so the prunella spike is often used for treating diseases such as thyromegaly, mastitis, hepatitis, hypertension and the like. Modern pharmacological researches find that the content of medicinal components is obviously related to the medicinal effect of the traditional Chinese medicinal materials, and the medicinal materials with high content of the medicinal components also have better treatment effect.
When the medicinal quality of the selfheal is improved, the traditional method usually adopts radiation irradiation for treatment, and the method has higher equipment requirement and more complex operation during treatment.
Disclosure of Invention
The invention aims to provide a method for improving the quality of selfheal medicinal materials by spraying melatonin, which can improve the growth capacity of selfheal, improve the content of traditional Chinese medicine components in fruit clusters and improve the quality of the medicinal materials.
The technical scheme adopted by the invention is as follows:
a method for improving the quality of selfheal medicinal materials by spraying melatonin comprises the following steps: spraying a melatonin solution of 50-200 mu mol/L to the selfheal plants during the flowering period of the selfheal plants.
Further, the concentration of the melatonin solution was 100. Mu. Mol/L.
Further, the process of spraying the melatonin solution is as follows: spraying melatonin solution 1 time every 1 day for three times.
Further, a potassium dihydrogen phosphate solution is sprayed once the next day of the first melatonin spraying, 1 time every 1 day, and 3 times in total.
Further, the mass concentration of the potassium dihydrogen phosphate solution is 0.2%.
Further, the spraying processes of the melatonin and the potassium dihydrogen phosphate solution are as follows: spraying is carried out at 6 pm, on the basis of the fact that the leaves are full of liquid drops.
Further, the preparation process of the melatonin solution comprises the following steps: melatonin was dissolved in ethanol and then diluted with deionized water.
Further, the method also comprises the following steps:
in 10 months, sowing the selfheal seeds in a nursery garden for seedling culture, transplanting the selfheal seedlings with consistent growth vigor into a flowerpot filled with garden soil and peat soil (the volume ratio is 3:1) which are mixed uniformly in the next 4 months, and placing the flowerpot in a greenhouse for culturing until the selfheal flowering period.
Further, the culture conditions in the greenhouse are as follows: the temperature is 26 +/-3 ℃ in the daytime, 20 +/-3 ℃ at night, the relative humidity is 70 +/-5 percent, and the water is normally managed.
The invention has the beneficial effects that:
1. according to the invention, melatonin with a specific concentration is sprayed to the selfheal during the flowering period of selfheal plants, the antioxidation performance, the photosynthetic capacity and the secondary metabolism capacity of the selfheal during the flowering period are improved by using the melatonin, the content of medicinal components in selfheal clusters can be effectively increased, and the medicinal material quality is improved.
2. When the melatonin is sprayed, the method that the spraying is carried out at intervals and the spraying is carried out alternately with the monopotassium phosphate is adopted, the monopotassium phosphate contains two elements of phosphorus and potassium and can supplement the potassium and phosphorus elements to plants simultaneously, the growth of the plant height, the ground diameter and the leaf length and width of the plants can be promoted, the branching amount can be increased, the chlorophyll content can be increased, the photosynthesis can be improved, the sugar accumulation in the plants can be promoted, the yield of crops can be increased, the melatonin has the effects of regulating an antioxidant system of the plants, improving the photosynthesis and the like and can also promote the absorption of the elements of phosphorus, potassium, mg, calcium and the like, the melatonin and the monopotassium phosphate are sprayed alternately, the plants can absorb the phosphorus and potassium elements better, the synergistic effect of the melatonin and the monopotassium phosphate is generated, and the growth of selfheal, the photosynthesis and the accumulation of the medicinal component content are effectively improved.
Drawings
FIG. 1 is a schematic diagram showing the effect of different melatonin treatment concentrations on the SOD content in Prunella vulgaris L.leaves;
fig. 2 is a schematic diagram showing the effect of different melatonin treatment concentrations on POD content in Prunella vulgaris leaves;
fig. 3 is a schematic diagram showing the effect of different melatonin treatment concentrations on the soluble sugar content in Prunella vulgaris leaves;
FIG. 4 is a schematic diagram showing the effect of different melatonin treatment concentrations on proline content in Prunella vulgaris leaves;
FIG. 5 is a schematic illustration of the effect of different melatonin treatment concentrations on the conductivity content in Prunella vulgaris leaves;
FIG. 6 is a schematic illustration of the effect of different melatonin treatment concentrations on MDA content in Prunella vulgaris leaves;
FIG. 7 is a graph showing the effect of different melatonin treatment concentrations on hydrogen peroxide content in Prunella vulgaris leaves;
FIG. 8 is a graph showing the effect of different melatonin treatment concentrations on Chla content in Prunella vulgaris leaves;
FIG. 9 is a graph showing the effect of different melatonin treatment concentrations on the Chlb content in Prunella vulgaris L.leaves;
FIG. 10 is a schematic view showing the effect of different melatonin treatment concentrations on the content of Car in Prunella vulgaris L leaves;
fig. 11 is a schematic diagram showing the effect of different melatonin treatment concentrations on Chla + b content in Prunella vulgaris leaves;
FIG. 12 is a graph showing the effect of different melatonin treatment concentrations on Pn in Prunella vulgaris leaves;
fig. 13 is a schematic diagram showing the effect of different melatonin treatment concentrations on Gs in Prunella vulgaris leaves;
fig. 14 is a graph showing the effect of different melatonin treatment concentrations on Ci in Prunella vulgaris leaves;
fig. 15 is a graph showing the effect of different melatonin treatment concentrations on Tr in Prunella vulgaris leaves.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
A method for improving the quality of selfheal medicinal materials by spraying melatonin comprises the following steps: spraying a melatonin solution of 50-200 mu mol/L to the selfheal plants during the flowering period of the selfheal plants.
Wherein the concentration of the melatonin solution is 100 mu mol/L.
Wherein, the process of spraying the melatonin solution comprises the following steps: spraying melatonin solution 1 time every 1 day for three times.
Wherein, the second day of melatonin spraying is to spray potassium dihydrogen phosphate solution once, and the spraying is performed for 1 time every 1 day and 3 times in total.
Wherein the mass concentration of the potassium dihydrogen phosphate solution is 0.2 percent.
Wherein, the spraying process of the melatonin and the potassium dihydrogen phosphate solution is as follows: spraying is carried out at 6 pm, on the basis of the fact that the leaves are full of liquid drops.
Wherein the preparation process of the melatonin solution comprises the following steps: melatonin was first dissolved in ethanol and then diluted with deionized water.
Wherein, still include the following step: in 10 months, sowing the selfheal seeds in a nursery garden for seedling culture, transplanting the selfheal seedlings with consistent growth vigor into a flowerpot filled with garden soil and peat soil (the volume ratio is 3:1) which are mixed uniformly in the next 4 months, and placing the flowerpot in a greenhouse for culturing until the selfheal flowering period.
Wherein, the culture conditions in the greenhouse are as follows: the temperature is 26 +/-3 ℃ in the daytime, 20 +/-3 ℃ at night, the relative humidity is 70 +/-5%, and the water is managed normally.
Example 1
(1) The method comprises the steps of disinfecting selfheal seeds by 2% of sodium hypochlorite, washing the selfheal seeds for three times by using clear water, sowing the selfheal seeds in a test field in the current 10 months for seedling culture, selecting seedlings with consistent growth vigor in the next 4 months, transplanting the seedlings into flowerpots filled with garden soil and peat soil (the volume ratio of the garden soil to the peat soil is 3:1) which are mixed uniformly, placing 4 plants of each pot plant in a greenhouse for culture, wherein the temperature is 26 +/-3 ℃ in the daytime, the temperature is 20 +/-3 ℃ at night, the relative humidity is 70 +/-5%, and normal water management is realized.
(2) Dissolving melatonin in proper amount of ethanol, diluting with deionized water to 50 mu mol/L, spraying melatonin solution onto plant in spray form in 5 months in normal condition, spraying 0.2% potassium dihydrogen phosphate in the second day after spraying melatonin solution, spraying 1 time in the first day and three times in the second day, and spraying at 6 pm when spraying all the components.
(3) Normally, in late 6 months, the spica prunellae are ripe, after the spica prunellae are ripe, the ripe spica prunellae are picked and collected, and the spica prunellae are dried in a drying oven at 50 ℃ until the weight is constant, so that a spica prunellae medicinal material, namely the dried spica prunellae.
Example 2
(1) The method comprises the steps of disinfecting selfheal seeds by 2% of sodium hypochlorite, washing the selfheal seeds for three times by using clear water, sowing the selfheal seeds in a test field in the current 10 months for seedling culture, selecting seedlings with consistent growth vigor in the next 4 months, transplanting the seedlings into flowerpots filled with garden soil and peat soil (the volume ratio of the garden soil to the peat soil is 3:1) which are mixed uniformly, placing 4 plants of each pot plant in a greenhouse for culture, wherein the temperature is 26 +/-3 ℃ in the daytime, the temperature is 20 +/-3 ℃ at night, the relative humidity is 70 +/-5%, and normal water management is realized.
(2) Dissolving melatonin in proper amount of ethanol, diluting with deionized water to 100 mu mol/L, spraying melatonin solution onto plant in spray form in 5 months in normal condition, spraying 0.2% potassium dihydrogen phosphate in the second day after spraying melatonin solution, spraying 1 time in the first day and three times in the second day, and spraying at 6 pm when spraying all the components.
(3) Normally, in late 6 months, the spica prunellae are ripe, after the spica prunellae are ripe, the ripe spica prunellae are picked and collected, and the spica prunellae are dried in a drying oven at 50 ℃ until the weight is constant, so that a spica prunellae medicinal material, namely the dried spica prunellae.
Example 3
(1) The method comprises the steps of disinfecting selfheal seeds by 2% of sodium hypochlorite, washing the selfheal seeds for three times by using clear water, sowing the selfheal seeds in a test field in the current 10 months for seedling culture, selecting seedlings with consistent growth vigor in the next 4 months, transplanting the seedlings into flowerpots filled with garden soil and peat soil (the volume ratio of the garden soil to the peat soil is 3:1) which are mixed uniformly, placing 4 plants of each pot plant in a greenhouse for culture, wherein the temperature is 26 +/-3 ℃ in the daytime, the temperature is 20 +/-3 ℃ at night, the relative humidity is 70 +/-5%, and normal water management is realized.
(2) Dissolving melatonin in proper amount of ethanol, diluting with deionized water to 200 mu mol/L, spraying melatonin solution onto plants in a spray mode in 5 months under normal conditions, spraying the plant once every 1 day for three times, spraying 0.2% monopotassium phosphate on the second day of spraying the melatonin solution for the first time, spraying 1 time every 1 day for three times, spraying at about 6 pm when all spraying operations are carried out, and taking the condition that leaves of the selfheal are full of liquid drops as the spraying finishing standard.
(3) Normally, in late 6 months, the prunella vulgaris clusters are ripe, after the prunella vulgaris clusters are ripe, picking and collecting the ripe prunella vulgaris clusters, and drying the prunella vulgaris clusters in a drying oven at 50 ℃ until the weight is constant to obtain the prunella vulgaris medicinal materials, namely the dried prunella vulgaris clusters.
Comparative example 1: different from the embodiment 1, when the prunella vulgaris in the flowering period is sprayed, deionized water is used for replacing the melatonin solution with the same spraying amount.
Comparative example 2: unlike example 1, the concentration of melatonin solution was 400 μmol/L when it was sprayed on prunella vulgaris in flowering period.
The selfheal obtained in the examples 1-3 and the comparative examples 1-2 is crushed and powdered, and after being screened by a 60-mesh sieve, the relevant medicinal components are determined by HPLC.
Fig. 1 to 4 are the results of measuring the contents of SOD, POD, soluble sugar and proline in Prunella vulgaris leaves at different melatonin treatment concentrations:
both SOD and POD activities exhibited increasing and decreasing trends with increasing concentrations of melatonin treatment, both reaching maximum values at concentrations of 100 μmol/L treatment, with 12.08% and 27.35% SOD and POD activities significantly increased over comparative example 1, followed by 200 μmol/L melatonin treatment with SOD and POD activities significantly increased over control by 8.01% and 21.86%, respectively, with SOD at 50 μmol/L and 400 μmol/L treatments higher than control but not significantly different from control, with POD at 50 μmol/L melatonin treatment significantly higher than control and POD at 400 μmol/L lower than control but not significantly different.
The content of soluble sugar and proline also shows the characteristic of increasing first and then decreasing along with the increase of the melatonin concentration, and the content of the soluble sugar and the proline in all melatonin treatment is higher than that in a control, wherein the content of the soluble sugar and the proline in 50-200 mu mol/L melatonin treatment reaches a significant level compared with that in comparative example 1, the content of the soluble sugar in 100 mu mol/L melatonin treatment and the content of the soluble sugar in 200 mu mol/L melatonin treatment are respectively 39.18% and 47.81% higher than that in the control, but the difference between the two treatments is not significant, the content of the proline in 100 mu mol/L melatonin treatment and the content of the proline in 200 mu mol/L melatonin treatment are respectively 70.88% and 47.16% higher than that in the control, the content of the soluble sugar and the proline in 100 mu mol/L melatonin treatment is significantly higher than that in 200 mu mol/L melatonin treatment, but the content of the soluble sugar and the proline in 400 mu mol/L melatonin treatment is higher than that in the control, but does not reach a significant level different from that in the control.
Fig. 5 to 7 are the measurement results of the electrical conductivity, MDA, hydrogen peroxide content in the prunella vulgaris leaves at different melatonin treatment concentrations: the conductivity of all treatments was lower than the control treatment, with 50-200 μmol/L melatonin treatment reaching significant levels, with the greatest decrease in conductivity for 100 μmol/L melatonin treatment, a 25-fold significant decrease over the control14% MDA content compared with H in comparison with comparative example 1 2 O 2 The content is remarkably reduced under 100-200 mu mol/L melatonin treatment, the reduction is not remarkable under 50 mu mol/L melatonin treatment and 400 mu mol/L melatonin treatment, and MDA and H under 100 mu mol/L melatonin treatment 2 O 2 The content is reduced by 15.30 percent and 24.57 percent respectively compared with the contrast.
Fig. 8 to 11 are the results of the determination of the chlorophyll and carotenoid contents in Prunella vulgaris leaves at different melatonin treatment concentrations: with the increase of the melatonin concentration, chla, chlb, car and Chl a + b of the selfheal under different treatments show a trend of increasing first and then decreasing, the increasing range of Chl a and Chl a + b contents is the largest under the treatment of 100 mu mol/L, the comparison ratio 1 is increased by 23.16 percent and 21.92 percent respectively, other melatonin treatments are higher than the comparison ratio but have no significant difference, chl b under all the treatments is higher than the comparison ratio 1 but does not reach a significant level compared with the comparison ratio 1, 50-200 mu mol/L melatonin treatment increases the content of the carotenoid of the selfheal leaf slices, wherein 100 mu mol/L and 200 mu mol/L melatonin are respectively and significantly increased by 24.74 percent and 20.18 percent compared with the comparison ratio, and 400 mu mol/L melatonin treatment has no significant difference compared with the comparison ratio 1.
Fig. 12 to 15 are measurement results of photosynthetic parameters in Prunella vulgaris leaves at different melatonin treatment concentrations: with the increase of the melatonin concentration, pn of the prunella vulgaris shows a trend of increasing first and then decreasing, except for 400 mu mol/L of the melatonin treatment, 50-200 mu mol/L of the melatonin treatment is obviously higher than that of the control, wherein Pn of 100 mu mol/L of the melatonin treatment is the highest, the Pn is obviously increased by 31.33% compared with that of the comparative example 1, then 200 mu mol/L of the melatonin treatment is obviously increased by 25.79% compared with that of the control, gs, ci and Tr of the prunella vulgaris are higher than those of the comparative example 1 under 50-200 mu mol/L of the melatonin treatment, three parameters under 100 mu mol/L and 200 mu mol/L of the melatonin treatment reach obvious levels compared with the comparative example 1, and three parameters under 400 mu mol/L of the melatonin treatment are not obviously different from that of the control.
Table 1 shows the results of the influence of melatonin treatment at different concentrations on the content of the ingredients used in the selfheal fruit-spike traditional Chinese medicine.
As can be seen from table 1, after spraying melatonin, the total flavone and total phenol contents were significantly higher than those of comparative example 1, both of which were the highest in 100 μmol/L melatonin treatment, wherein the total flavone contents of 100 μmol/L and 200 μmol/L melatonin treatment were significantly increased by 24.87% and 13.91% respectively, the total phenol contents of both treatments were significantly increased by 72.74% and 39.36% respectively, the medicinal ingredient caffeic acid, rosmarinic acid and hyperoside in 50-200 μmol/L melatonin treatment were higher than those of comparative example 1, both of which were maximally increased in 100 μmol/L melatonin treatment, and secondly, the caffeic acid and hyperoside in 400 μmol/L melatonin treatment were higher than those of comparative example 1, but the comparative example was not significantly different from comparative example 1, the rosmarinic acid content in 400 μmol/L melatonin treatment was lower than that of comparative example 1, but was not significantly different from that of 321, the caffeic acid and the hyperoside in 100 μmol/L melatonin treatment was increased by 24.62% and 96.62%.
It should be noted that the above embodiments are only for illustrating the present invention, but the present invention is not limited to the above embodiments, and any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention fall within the protection scope of the present invention.
Claims (9)
1. A method for improving the quality of selfheal medicinal materials by spraying melatonin is characterized by comprising the following steps: spraying a melatonin solution of 50-200 mu mol/L to the selfheal plants during the flowering period of the selfheal plants.
2. The method for improving the quality of selfheal medicinal materials by spraying melatonin as claimed in claim 1, wherein the concentration of the melatonin solution is 100 μmol/L.
3. The method for improving the quality of the selfheal medicinal material by spraying the melatonin as claimed in claim 1, wherein the spraying of the melatonin solution comprises the following steps: the melatonin solution is sprayed for 1 time every 1 day and is sprayed for three times in total.
4. The method for improving the quality of selfheal medicinal materials by spraying melatonin as claimed in claim 3, wherein the melatonin is sprayed once on the second day after the melatonin is sprayed for the first time, and the spraying is performed for 3 times every 1 day.
5. The method for improving the quality of selfheal medicinal materials by spraying melatonin as claimed in claim 4, wherein the mass concentration of the potassium dihydrogen phosphate solution is 0.2%.
6. The method for improving the quality of selfheal medicinal materials by spraying melatonin as claimed in any one of claims 1 to 5, wherein the spraying processes of the melatonin and the potassium dihydrogen phosphate solution are as follows: spraying is carried out at 6 pm, on the basis of the fact that the leaves are full of liquid drops.
7. The method for improving the quality of the prunella vulgaris medicinal material according to claim 1, wherein the preparation process of the melatonin solution comprises the following steps: melatonin is taken and dissolved in ethanol, and then diluted by deionized water.
8. The method for improving the quality of the prunella vulgaris medicinal material by spraying melatonin as claimed in claim 1, further comprising the following steps:
in 10 months, sowing the selfheal seeds in a nursery garden for seedling culture, transplanting the selfheal seedlings with consistent growth vigor into a flowerpot filled with garden soil and peat soil (the volume ratio is 3:1) which are mixed uniformly in the next 4 months, and placing the flowerpot in a greenhouse for culturing until the selfheal flowering period.
9. The method for improving the quality of the selfheal medicinal material by spraying the melatonin as claimed in claim 8, wherein the culture conditions in a greenhouse are as follows: the temperature is 26 +/-3 ℃ in the daytime, 20 +/-3 ℃ at night, the relative humidity is 70 +/-5%, and the water is managed normally.
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