AU2020103856A4 - A method for improving drought resistance of mustard - Google Patents

A method for improving drought resistance of mustard Download PDF

Info

Publication number
AU2020103856A4
AU2020103856A4 AU2020103856A AU2020103856A AU2020103856A4 AU 2020103856 A4 AU2020103856 A4 AU 2020103856A4 AU 2020103856 A AU2020103856 A AU 2020103856A AU 2020103856 A AU2020103856 A AU 2020103856A AU 2020103856 A4 AU2020103856 A4 AU 2020103856A4
Authority
AU
Australia
Prior art keywords
drought
mustard
melatonin
seedlings
stress
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2020103856A
Inventor
Li Gong
Jie Li
Mengyao LI
Yanwen Li
Fangjie Xie
Yu Zhang
Jin Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Agricultural University
Original Assignee
Sichuan Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Agricultural University filed Critical Sichuan Agricultural University
Priority to AU2020103856A priority Critical patent/AU2020103856A4/en
Application granted granted Critical
Publication of AU2020103856A4 publication Critical patent/AU2020103856A4/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Soil Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention belongs to the technical field of agricultural production and provides a method for improving the drought resistance of mustard. The mustard variety 'Fuza No.2' is used as plant material, and different concentrations of melatonin are sprayed on the seedling leaves while PEG6000 is used to simulate drought stress. To determine the effects of melatonin on the stress resistance and oxidative system of mustard seedlings at different drought stress. The measurement of physiological indicators of seedlings at six different time periods showed that under drought stress, foliar spraying with melatonin significantly improved the traits of seedlings, increased the content of total soluble sugar, and increased the activity of antioxidant enzymes and effectively reduce the accumulation of MDA substances. Among them, D+M100 (drought + sprayed 100 umol/LMT) treatment is better than D+M200 (drought + sprayed 200 umol/LMT) in increasing stem thickness, plant height, fresh weight and water content of above-ground part, total soluble sugar content, and SOD enzyme activity. Compared with D+M100, D+M200 has a more significant effect in improving POD enzyme activity in the early stage in drought stress, and the effect of D+M100 treatment is more obvious in the later stage. This comprehensively indicates that spraying the exogenous melatonin can effectively improve the physiological function of mustard seedlings under drought stress and increase the enzyme activity of the antioxidant system, and the improvement effect of melatonin at the concentration of 100 umol/L is better than that of 200 umol/L. According to these drought resistance indicators, the adaptive concentration range of spraying melatonin is determined, which solves the dilemma that mustard is facing drought stress in cultivation, and has strong application value.

Description

A method for improving drought resistance of mustard
TECHNICAL FIELD
[01] The invention belongs to the technical field of crop planting, and specifically relates to a method for improving the drought resistance of mustard.
BACKGROUND
[02] Stem mustard, belongs to the Brassica genus of the cruciferous family and is a variety of mustard with fleshy stems as a product. Stem mustard is a Chinese specialty vegetable. The raw material of the well-known "Fuling mustard" at home and abroad is stem mustard, which is mainly grown in Chongqing, Sichuan and other southwest regions in China. Stem mustard has the advantages of high yield, good quality, multiple utilization methods, strong palatability, high planting efficiency, and great production potential. Mustard like a cold and humid environment, avoid heat and drought. Mustard is suitable for planting in neutral loam with deep soil, good irrigation and drainage conditions, good permeability, water and fertilizer retention. If the soil lacks water or drought, it will cause serious yield reduction and poor quality.
[03] With global warming, drought has become a common natural disaster and one of the most important stress factors affecting plant physiology and biochemistry. Studies on drought resistance identification indicators show that drought stress not only wilts the morphology of plants, but also causes free proline, a large accumulation of soluble sugar, decreased water potential, increased membrane permeability and membrane lipid peroxidation product MDA. Southwest China, which is one of the main producing areas of mustard, is affected by comprehensive factors such as monsoon climate and topography (the altitude decreases from northwest to southeast), and there are obvious differences in agricultural production conditions. Judging from the distribution of major meteorological disasters in Southwest China, this region is one of the most frequent drought disasters in China. Drought has always been a bottleneck restricting the development of mustard industrialization in south-western of China. The growth of mustard is often affected, often resulting in a substantial reduction in production. Therefore, improving the drought resistance of mustard has become one of the important methods of agricultural planting.
[04] Melatonin (Melatonin) N-acetyl-5-methoxytryptamine is a pleiotropic and highly conserved molecule that exists widely in plants and animals. Melatonin is considered to be an endogenous free radical scavenger, which can effectively scavenge H202. In recent years, studies have shown that an important function of melatonin in plants is to act as the first line of defense against oxidative stress in the internal and external environment. Melatonin can effectively improve the drought tolerance of plants. Its main mechanism is to remove the oxidative stress caused by drought stress by increasing the content of active oxygen scavenging enzymes and antioxidant substances, so as to enhance the drought resistance of plants.
[05] The present invention provides a method for improving the drought resistance of mustard, which aims to eliminate the oxidative stress caused by drought stress by increasing the content of active oxygen scavenging enzymes and antioxidant substances, thereby enhancing the drought resistance of plants. That is, 10% PEG6000 is used to cause the physiological drought of the crops, and at the same time, different concentrations of melatonin are sprayed on the leaves to determine the effects of melatonin on the stress resistance physiological and oxidative system of mustard seedlings at different drought stress time to observe whether the plants can grow normally development, combined with the determination of some indicators to identify the drought resistance at the seedling stage, determine the adaptive concentration range of spraying melatonin, and provide a theoretical basis for the drought-resistant cultivation of mustard and increase the yield.
[06] The invention adopts the method of spraying melatonin on the foliar surface, and the obtained results are relatively reliable, with less workload, good repeatability, small error, simple and convenient use, reducing toxic and side effects, and reducing environmental pollution, thereby solving the dilemma of field mustard facing drought. The invention greatly simplifies the drought resistance methods and means, has very important meanings for improving the drought resistance and increasing the yield of mustard, and the ecological benefits, economic benefits and social benefits are very significant.
SUMMARY
[07] The present invention belongs to the technical field of agricultural production and provides a method for improving the drought resistance of mustard. The purpose is to provide a method for quickly improving the drought resistance of mustard. The method is to measure the effects of mustard with different concentrations of melatonin under drought stress. The physiology and oxidative system of mustard, among them, melatonin with a concentration of 100umol/L has the best drought resistance of mustard varieties.
[08] Using the 'Fuza No.2' mustard variety as plant material, spraying different concentrations of melatonin on the leaves and simultaneously using PEG6000 to simulate drought stress was used to determine the effects of melatonin on the stress resistance physiological and oxidative systems of mustard seedlings at different drought stress times. In this experiment, drought stress reduced the fresh weight of the above ground part and the water content of the above-ground part, reduced the seedling stem thickness and plant height, and the effect of the above-ground water content was more obvious. The treatment of foliar spraying with melatonin significantly improved the traits of seedlings under drought stress, maintained the basic morphology of the seedlings and improved the growth status. And under drought stress, D+M100 treatment had better effect than D+M200 in increasing stem thickness, plant height, and increasing fresh weight and water content of above-ground part.
[09] Drought stress will break the PSII electron transfer balance. In order to dissipate the excessive light energy, the excess electrons are transferred to molecular oxygen, thereby producing ROS under the corresponding reaction coupler. The accumulation of ROS will lead to membrane lipid peroxidation, chlorophyll degradation, Destroy cell membrane integrity and reduce photosynthesis. Plants will increase antioxidant enzyme activity under adversity conditions to prevent ROS damage, but severe drought stress will reduce antioxidant enzyme activity, which will induce a decrease in antioxidant enzyme activity and weaken intracellular enzymes The ability to remove harmful substances from active oxygen, which in turn causes a large accumulation of harmful substances MDA, which in turn destroys the structure and function of the membrane. In this experiment, drought stress led to the accumulation of MDA in seedlings. Spraying melatonin on leaves significantly reduced the content of
MDA, significantly increased the content of total soluble sugar, increased the content of osmotic adjustment substances in seedling cells, reduced oxidative damage, and effectively reduced water loss. Within 15 days, the malondialdehyde content of the seedlings treated with D+M100 was 0.94, 0.77, 1.15, 0.8, 0.8, 1.08 times that of the D+M200 treatment. The total soluble sugar content of the seedlings treated with D+M100 was D+ M200 treatment is 1.01 times, 1.18 times, 1.19 times, 1.20 times, 1.12 times, 1.07 times, indicating that melatonin can protect the integrity of cell membranes under drought stress, and D+M100 has a better effect.
[010] With the increase of drought stress, in order to prevent the accumulation of harmful substances in plants, remove excess reactive oxygen species, malondialdehyde and other substances, and reduce the damage of plant cell membranes, the activity of antioxidant enzymes such as SOD, CAT, and POD will increased to reduce harmful substances. Decompose and eliminate its toxic effects. In this experiment, foliar spraying with melatonin significantly increased the activity of antioxidant enzymes and effectively reduced the accumulation of MAD substances, indicating that melatonin can improve the cell redox balance by increasing the antioxidant enzyme activity of mustard seedlings, thereby protect cells from oxidative damage under drought stress. From day 3 to day 15 of experimental sampling, the SOD enzyme activity of D+M100 treated seedlings was 1.02 times, 1.06 times, 1.03 times, and 0.99 times higher than that of D+M200; from day 6 to day 12 of experimental sampling, D+M200 treated The POD enzyme activity of seedlings was 1.23 times and 1.69 times higher than that of D+M100. From day 9 to day 12, the enzyme activity of seedlings treated with D+M100 increased sharply, and the enzyme activity of seedlings treated with D+M200 decreased. The enzyme activities of D+M100 and D+M200 were basically the same on day 12, and the POD enzyme activity of D+M100 treatment was 1.25 times that of D+M200 treatment on the 15th day.
[011] The research of the above test point shows that spraying the exogenous hormone melatonin can effectively improve the physiological function of mustard seedlings under drought stress and increase the enzyme activity of the antioxidant system, and the improvement effect of melatonin at a concentration of 100 umol/L is better than that of 200 umol/L is better. According to these drought resistance indicators, the adaptive concentration range of spraying melatonin is determined, which solves the dilemma of the mustard cultivation facing drought stress, and has strong promotion and application value.
BRIEF DESCRIPTION OF THE FIGURES
[012] Figure 1 Changes in stem thickness of mustard seedlings in different treatments and stages after PEG stress
[013] Figure 2 Changes in plant height of mustard seedlings in different treatments and stages after PEG stress
[014] Figure 3 Changes in fresh weight of the above-ground part of mustard seedlings in different treatments and stages after PEG stress
[015] Figure 4 Changes in the above ground water content of mustard seedlings in different treatments and different periods after PEG stress
[016] Figure 5 Changes of malondialdehyde in mustard seedlings at different treatments and different stages after PEG stress
[017] Figure 6 Changes of total soluble sugars in mustard seedlings at different treatments and stages after PEG stress
[018] Figure 7 Changes of superoxide dismutase activity in mustard seedlings at different treatments and stages after PEG stress
[019] Figure 8 Changes in peroxidase activity of mustard seedlings in different treatments and different periods after PEG stress
DESCRIPTION OF THE INVENTION
[020] The present invention belongs to the technical field of agricultural production and provides a method for improving the drought resistance of mustard. The purpose is to provide a method for quickly improving the drought resistance of mustard. The method is to measure the effects of mustard with different concentrations of melatonin under drought stress. The physiology and oxidative system of mustard, among them, melatonin with a concentration of 100umol/L has the best drought resistance of mustard varieties.
[021] Using the 'Fuza No.2' mustard variety as plant material, spraying different concentrations of melatonin on the leaves and simultaneously using PEG6000 to simulate drought stress was used to determine the effects of melatonin on the stress resistance physiological and oxidative systems of mustard seedlings at different drought stress times. In this experiment, drought stress reduced the fresh weight of the above ground part and the water content of the above-ground part, reduced the seedling stem thickness and plant height, and the effect of the above-ground water content was more obvious. The treatment of foliar spraying with melatonin significantly improved the traits of seedlings under drought stress, maintained the basic morphology of the seedlings and improved the growth status. And under drought stress, D+M100 treatment had better effect than D+M200 in increasing stem thickness, plant height, and increasing fresh weight and water content of above-ground part.
[022] Drought stress will break the PSII electron transfer balance. In order to dissipate the excessive light energy, the excess electrons are transferred to molecular oxygen, thereby producing ROS under the corresponding reaction coupler. The accumulation of ROS will lead to membrane lipid peroxidation, chlorophyll degradation, Destroy cell membrane integrity and reduce photosynthesis. Plants will increase antioxidant enzyme activity under adversity conditions to prevent ROS damage, but severe drought stress will reduce antioxidant enzyme activity, which will induce a decrease in antioxidant enzyme activity and weaken intracellular enzymes The ability to remove harmful substances from active oxygen, which in turn causes a large accumulation of harmful substances MDA, which in turn destroys the structure and function of the membrane. In this experiment, drought stress led to the accumulation of MDA in seedlings. Spraying melatonin on leaves significantly reduced the content of MDA, significantly increased the content of total soluble sugar, increased the content of osmotic adjustment substances in seedling cells, reduced oxidative damage, and effectively reduced water loss. Within 15 days, the malondialdehyde content of the seedlings treated with D+M100 was 0.94, 0.77, 1.15, 0.8, 0.8, 1.08 times that of the D+M200 treatment. The total soluble sugar content of the seedlings treated with D+M100 was D+ M200 treatment is 1.01 times, 1.18 times, 1.19 times, 1.20 times, 1.12 times, 1.07 times, indicating that melatonin can protect the integrity of cell membranes under drought stress, and D+M100 has a better effect.
[023] With the increase of drought stress, in order to prevent the accumulation of harmful substances in plants, remove excess reactive oxygen species, malondialdehyde and other substances, and reduce the damage of plant cell membranes, the activity of antioxidant enzymes such as SOD, CAT, and POD will increased to reduce harmful substances. Decompose and eliminate its toxic effects. In this experiment, foliar spraying with melatonin significantly increased the activity of antioxidant enzymes and effectively reduced the accumulation of MAD substances, indicating that melatonin can improve the cell redox balance by increasing the antioxidant enzyme activity of mustard seedlings, thereby protect cells from oxidative damage under drought stress. From day 3 to day 15 of experimental sampling, the SOD enzyme activity of D+M100 treated seedlings was 1.02 times, 1.06 times, 1.03 times, and 0.99 times higher than that of D+M200; from day 6 to day 12 of experimental sampling, D+M200 treated The POD enzyme activity of seedlings was 1.23 times and 1.69 times higher than that of D+M100. From day 9 to day 12, the enzyme activity of seedlings treated with D+M100 increased sharply, and the enzyme activity of seedlings treated with D+M200 decreased. The enzyme activities of D+M100 and D+M200 were basically the same on day 12, and the POD enzyme activity of D+M100 treatment was 1.25 times that of D+M200 treatment on the 15th day.
[024] The research of the above test point shows that spraying the exogenous hormone melatonin can effectively improve the physiological function of mustard seedlings under drought stress and increase the enzyme activity of the antioxidant system, and the improvement effect of melatonin at a concentration of 100 umol/L is better than that of 200 umol/L is better. According to these drought resistance indicators, the adaptive concentration range of spraying melatonin is determined, which solves the dilemma of the mustard cultivation facing drought stress, and has strong promotion and application value.
[025] Although the invention has been described with reference to specific examples, it will be appreciated by those skilled in the art that the invention may be embodied in many other forms, in keeping with the broad principles and the spirit of the invention described herein.
[026] The present invention and the described embodiments specifically include the best method known to the applicant of performing the invention. The present invention and the described preferred embodiments specifically include at least one feature that is industrially applicable

Claims (3)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:
1. Eliminate the oxidative stress caused by drought stress by increasing the content of active oxygen scavenging enzymes and antioxidants. Using 10% PEG6000 can cause physiological drought of crops, while spraying different concentrations of melatonin on the leaves to achieve enhancement the resistance of plants to drought.
2. The method of spraying melatonin on the foliar surface has less workload, good repeatability, small error, simple and easy to implement, and can be carried out in large quantities.
3. Simplified drought resistance methods and means are of great significance for improving drought resistance and yield of mustard. The ecological, economic and social benefits are very significant, no toxic side effects, and can reduce environmental pollution, thereby solving the problem of drought in field mustard.
AU2020103856A 2020-12-03 2020-12-03 A method for improving drought resistance of mustard Ceased AU2020103856A4 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2020103856A AU2020103856A4 (en) 2020-12-03 2020-12-03 A method for improving drought resistance of mustard

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU2020103856A AU2020103856A4 (en) 2020-12-03 2020-12-03 A method for improving drought resistance of mustard

Publications (1)

Publication Number Publication Date
AU2020103856A4 true AU2020103856A4 (en) 2021-02-11

Family

ID=74502345

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020103856A Ceased AU2020103856A4 (en) 2020-12-03 2020-12-03 A method for improving drought resistance of mustard

Country Status (1)

Country Link
AU (1) AU2020103856A4 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113016389A (en) * 2021-03-18 2021-06-25 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN113742947A (en) * 2021-10-12 2021-12-03 宁夏大学 Method for determining field planting water threshold of warm-zone desert grassland plant seedlings
CN114868592A (en) * 2022-06-06 2022-08-09 内蒙古农业大学 Application of cytokinin in relieving drought stress of cerasus humilis
CN115259138A (en) * 2022-08-04 2022-11-01 江南大学 Method for improving drought resistance and yield of crops based on carbon nano material
CN115413536A (en) * 2022-09-27 2022-12-02 河南科技大学 Method for improving quality of selfheal medicinal material by spraying melatonin
CN115504828A (en) * 2022-09-02 2022-12-23 广东省农业科学院蔬菜研究所 Method for improving low calcium stress resistance of wax gourds
CN116616138A (en) * 2023-07-13 2023-08-22 四川省中医药科学院 Screening method of drought-resistant germplasm of ophiopogon japonicus
CN117158251A (en) * 2023-09-21 2023-12-05 山东省农业科学院 Method for promoting growth recovery of tea trees at low temperature

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113016389A (en) * 2021-03-18 2021-06-25 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN113016389B (en) * 2021-03-18 2022-05-20 甘肃省科学院生物研究所 Application of melatonin in influencing microbial diversity of rhizosphere soil of medicinal plants
CN113742947A (en) * 2021-10-12 2021-12-03 宁夏大学 Method for determining field planting water threshold of warm-zone desert grassland plant seedlings
CN113742947B (en) * 2021-10-12 2024-05-03 宁夏大学 Method for determining planting water threshold of young plants in temperate desert grassland
CN114868592A (en) * 2022-06-06 2022-08-09 内蒙古农业大学 Application of cytokinin in relieving drought stress of cerasus humilis
CN115259138B (en) * 2022-08-04 2023-09-22 江南大学 Method for improving drought tolerance and yield of crops based on carbon nanomaterial
CN115259138A (en) * 2022-08-04 2022-11-01 江南大学 Method for improving drought resistance and yield of crops based on carbon nano material
CN115504828A (en) * 2022-09-02 2022-12-23 广东省农业科学院蔬菜研究所 Method for improving low calcium stress resistance of wax gourds
CN115504828B (en) * 2022-09-02 2023-09-05 广东省农业科学院蔬菜研究所 Method for improving low-calcium stress resistance of white gourd
CN115413536A (en) * 2022-09-27 2022-12-02 河南科技大学 Method for improving quality of selfheal medicinal material by spraying melatonin
CN115413536B (en) * 2022-09-27 2024-04-05 河南科技大学 Method for improving quality of selfheal medicinal material by melatonin spraying
CN116616138A (en) * 2023-07-13 2023-08-22 四川省中医药科学院 Screening method of drought-resistant germplasm of ophiopogon japonicus
CN116616138B (en) * 2023-07-13 2024-02-09 四川省中医药科学院 Screening method of drought-resistant germplasm of ophiopogon japonicus
CN117158251A (en) * 2023-09-21 2023-12-05 山东省农业科学院 Method for promoting growth recovery of tea trees at low temperature

Similar Documents

Publication Publication Date Title
AU2020103856A4 (en) A method for improving drought resistance of mustard
CN106613702B (en) Method for relieving drought stress of kiwi fruit trees
CN105646081B (en) Liquid compound fertilizer, preparation method and application thereof
CN113748947B (en) Method for promoting root growth of perennial sugarcane after mechanical harvesting
CN100425142C (en) Water melon sweet-increasing accelerant and the spraying method
CN105145570A (en) Composite capable of improving plant freezing resistance and preparation method thereof
Kuroda et al. Changes in hydrogen peroxide content in flower buds of Japanese pear (Pyrus pyrifolia Nakai) in relation to breaking of endodormancy
CN110973155A (en) Cold-proof agent for wheat late spring coldness
CN114287423A (en) Composition for preventing and treating gray mold of Paris polyphylla, and use method and application thereof
CN102173938A (en) Novel active multi-composite microfertilizer and preparation method thereof
CN103125520A (en) Conditioning agent for low temperature resistance corn strong stalk production increasing and quality improving and preparation method and application thereof
CN113207452A (en) Method for relieving drought stress of grape nursery stocks
CN115968884B (en) Plant antifreeze agent containing curcumin and raffinose as well as preparation method and application thereof
CN108925583B (en) High-temperature-resistant preparation for rice and use method thereof
CN1672496A (en) Plant-antifreezing liquid and its prepn
CN112930928A (en) Protective agent for relieving toxicity of atmospheric ozone pollution to sensitive plants and using method
CN113348956A (en) Method for relieving copper stress of grape seedlings
CN112450008A (en) Continuous cropping planting method for watermelons
TWI639579B (en) Algae broken wall ferment as foliar fertilizer and enhance leaf crops against mites use
CN113973821B (en) Drought-resistant agent and method for improving drought resistance of plants
CN117178990B (en) Novel application of novel compound in improving drought stress resistance of plants
CN114868592A (en) Application of cytokinin in relieving drought stress of cerasus humilis
CN117467200B (en) Antifreezing compound liquid for plant growth and preparation method and application thereof
CN116941639B (en) Application of sodium nitroprusside in relieving high temperature heat injury in flowering phase of rice
Hu et al. Effects of different buckle shed time on catalase activity and malondialdehyde content in grape leaves

Legal Events

Date Code Title Description
FGI Letters patent sealed or granted (innovation patent)
MK22 Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry