CN106106297B - Artificial breeding method for rhodeus bitterling - Google Patents
Artificial breeding method for rhodeus bitterling Download PDFInfo
- Publication number
- CN106106297B CN106106297B CN201610608318.7A CN201610608318A CN106106297B CN 106106297 B CN106106297 B CN 106106297B CN 201610608318 A CN201610608318 A CN 201610608318A CN 106106297 B CN106106297 B CN 106106297B
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- fish
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- rhodeus
- water
- bitterling
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- 241000594026 Rhodeus Species 0.000 title claims abstract description 30
- 238000009395 breeding Methods 0.000 title claims abstract description 30
- 241000251468 Actinopterygii Species 0.000 claims abstract description 60
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 235000013601 eggs Nutrition 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 9
- 239000004568 cement Substances 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 241001290772 Limnodrilus Species 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 210000003128 head Anatomy 0.000 claims description 3
- 230000009027 insemination Effects 0.000 claims description 3
- 210000003205 muscle Anatomy 0.000 claims description 3
- 210000003101 oviduct Anatomy 0.000 claims description 3
- 230000032696 parturition Effects 0.000 claims description 3
- 241000256248 Spodoptera Species 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 230000016087 ovulation Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 241000237536 Mytilus edulis Species 0.000 abstract description 4
- 235000020638 mussel Nutrition 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 3
- 239000013505 freshwater Substances 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000006698 induction Effects 0.000 abstract description 2
- 241000208365 Celastraceae Species 0.000 abstract 1
- 235000000336 Solanum dulcamara Nutrition 0.000 abstract 1
- 239000008187 granular material Substances 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241001275931 Rhodeus ocellatus Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
An artificial breeding method for rhodeus bitterling comprises the steps of collecting wild rhodeus bitterling breed fish from the beginning of 6 months, stocking and breeding the breed fish, preparing an oxytocic, carrying out artificial induced spawning on the breed fish, carrying out artificial fertilization, hatching and the like. The method for cultivating rhodeus bittersweet has the advantages of high speed, spawning induction rate of over 80 percent, fertilization rate of over 80 percent and fry survival rate of over 60 percent. Particularly, the bitterling does not need freshwater mussels for culturing the rhodeus by adopting the method, so that the limitation of the environment is reduced.
Description
Technical Field
The invention belongs to the ornamental fish culture technology, and particularly relates to an artificial breeding method of rhodeus ocellatus.
Background
With the rapid improvement of the living standard of people, people have stronger pursuit on spiritual and physical life, the ornamental fish is promoted to approach thousands of households, and the life of people is promoted to be richer and more colorful. Bitterling a variety of rhodeus, a variety of aquarium fish, (a) bitterling a variety of rhodeusRhoaeus lighti) Cucurbit seeds, cucurbit flakes and poplar leaves are named separately, belong to the order cypriniformes, the family cyprinidae, the subidae rhodeus, and the genus rhodeus, and are mainly distributed in various water systems such as lujiang, Liaohe and northern Heilongjiang to Fujian and Guangdong in China. Inhabit in the water bay where the water flow is slow and the aquatic plants are flourishing. The fish has high ornamental value, but the quantity is small, the market demand can not be met, the freshwater mussel is required for artificial cultivation, the technology is complex, the survival rate is low, and the cost is high.
Disclosure of Invention
The invention aims to provide an artificial breeding method for rhodeus bitterling, which is high in breeding speed, high in quality of cultured rhodeus bitterling and low in cost.
The technical scheme is as follows:
an artificial breeding method of rhodeus bitterling is characterized by comprising the following steps:
1. collection and cultivation of variety fish of various stone:
beginning to collect rhodeus paludiana parent fish in the beginning of sixty months: the female fish is 6.0-12.0 cm long, 5.0-25.0 g in weight and more than 3 years old, and has an oviduct on the abdomen; the length of the male fish body is 5.0-10.0 cm, the weight is 5.0-15.0 g, the body is more than 2 years old, the body is bright in color, and the head, mouth and eyes and the lower jaw are provided with the star-chasing. The number ratio of the male fish to the female fish is 2-3: 1.
2. Stocking and breeding the parent fishes:
placing the selected breeding fishes into a temporary culture cement pond, wherein the density is 100 plus 150 fish per square meter, the water depth is 40-60cm, and the water temperature is controlled to be 15-25 ℃. After the breeding fish enters the pond, slowly feeding the breeding fish for 1-2 days, wherein the bait is combined by granular feed and limnodrilus, the feeding amount accounts for about 3-5% of the weight of the breeding fish, and water is injected and changed once every 3 days during the breeding period.
3. Preparing an oxytocic drug: the oxytocic is 15ug of ovulation promoting agent 2 and 15mg of diulon per kg, and the male fish is 1/2 female fish.
4. Hastening parturition: injecting the oxytocic prepared in the third step into the muscle at the base of dorsal fin of the parent fish, and separately putting the injected parent fish into the temporary rearing pond; and raising the temperature of the temporary culture pond to 20-25 ℃.
5. Artificial insemination: and (3) extruding egg granules from female fish 20-28 hours after induced spawning, mixing the eggs and the sperm, and performing dry fertilization to obtain fertilized eggs.
6. Hatching: and (3) carrying out oxygen-enriched incubation by using pure water, wherein the water temperature is 18-23 ℃, a sunshade net is arranged above the incubation pool for shading treatment, the dissolved oxygen in the water is kept at more than 5mg/L, and dead eggs are removed every day during incubation. And (3) injecting and changing water once a day, injecting and changing water 2-3 times a day after 3 days, and culturing the fries in a cement pond after the fries are black. And (4) the accumulated temperature of rhodeus in the incubation period needs 36-45 days, and the fries of spodoptera spongiensis are obtained after 13-15 days.
The invention has the advantages that:
the invention mainly solves the complicated problems that the existing bitterling seedlings need to be fished in a natural water area, or mussels need to participate in artificial spawning, and the like. The artificial breeding technology for bitterling the rhodeus bitterling is successfully summarized, freshwater mussels are not needed in the technology, the technology reduces the environmental requirement, the whole technical process is implemented in a controllable range, the influence of the external environment is small, the operation is convenient and reliable, the difficult problem of fry production of the bitterling the rhodeus bitterling is well solved, and the method has important significance for wild resource protection and large-scale cultivation of the bitterling the rhodeus bitterling. By applying the technology, the sponginess rate of rhodeus bitterling is over 80%, the fertilization rate is over 80%, the hatching rate is over 80%, and the survival rate of seedlings is over 60%.
Detailed Description
Example one
An artificial breeding method of rhodeus bitterling comprises the following steps:
1. collecting breeding fishes:
collecting wild female fish: the body length is 6-12 cm, the weight is 5-15g, the body is born for three years, and the abdomen has an oviduct; the male fish has a length of 5-10cm, a weight of 5-15g, two years old, a colorful and fresh body, the head, mouth and eyes have star-chasing, and the number ratio of the male fish to the female fish is 2: 1.
2. Stocking and culturing parent fishes:
placing the selected breeding fish in a temporary culture cement pond, wherein the density of the breeding fish is 100-2The water depth is 40 cm, the water temperature is controlled at 15-20 deg.C, after 1-2 days, feeding bait for the breeding fish, the bait is composed of artificially prepared granular feed and limnodrilus, the input amount is 3-5% of fish weight, and water is changed every 3 days during the cultivation period.
3. Preparing oxytocic and hastening parturition
The oxytocic is mixed with 15ug of ovulation-promoting hormone 2 and 15mg of diohuone per kilogram to prepare the oxytocic, the dosage of the male fish is 1/2 of the female fish, the prepared oxytocic is injected into the muscle at the base of the dorsal fin of the parent fish, then the female and male fish are separated and put back into a temporary culture pond, and the water temperature is 20-25 ℃.
4. Artificial fertilization:
2-28 hours after spawning induction, the female fish extrudes out egg granules, and the sperm and the eggs are mixed to obtain fertilized eggs.
5. Hatching:
adopting a pure water oxygenation hatching method, keeping the water temperature at 18-23 ℃, building a sunshade net above a hatching pond, keeping the dissolved oxygen in the water at more than 5mg/l, and picking out dead eggs every day during hatching. And (3) injecting water once a day, injecting water 3 times a day three days later, putting the fries into a cement pond for breeding after the fries become black, and obtaining the fries of the lotuses pani after the fries are incubated for 36-45 degrees in incubation period and 13-15 days later.
Example two
The artificial culture method of bitterling in the second embodiment is basically the same as that in the first embodiment, and the difference is that:
the weight of the female fish is 15-25g, and the number of the male fish and the female fish is 3: 1.
The stocking density of the breeding fishes is 150 pieces per square meter together with 120 pieces per square meter. The water temperature of the cultured breeding fish is controlled to 18-25 ℃.
Claims (1)
1. An artificial breeding method of rhodeus bitterling is characterized by comprising the following steps:
(1) collection and cultivation of variety fish of various stone:
beginning to collect rhodeus paludiana parent fish in the beginning of sixty months: the female fish is 6.0-12.0 cm long, 5.0-25.0 g in weight and more than 3 years old, and has an oviduct on the abdomen; the male fish is 5.0-10.0 cm in length, 5.0-15.0 g in weight and more than 2 years old, the body is bright in color, the head, mouth and eyes and the lower jaw are provided with chasing stars, and the number ratio of the male fish to the female fish is 2-3: 1;
(2) stocking and breeding the parent fishes:
placing the selected breeding fishes into a temporary-culture cement pond, wherein the density is 100 plus 150 fish per square meter, the water depth is 40-60cm, and the water temperature is controlled to be 15-25 ℃; after the breeding fish enters the pond, slowly feeding the breeding fish for 1-2 days, wherein the bait is combined by granular feed and limnodrilus, the feeding amount accounts for about 3-5% of the weight of the breeding fish, and water is injected and changed once every 3 days during the breeding period;
(3) preparing an oxytocic drug: the oxytocic and the dosage are 15ug of ovulation promoting hormone 2 and 15mg of diohuone per kilogram, and the dosage of male fish is 1/2;
(4) hastening parturition: injecting the oxytocic prepared in the step (3) into the dorsal fin basal muscles of the parent fish, and separately placing the injected parent fish males and females back into the temporary culture pond; raising the temperature of the temporary culture pond to 20-25 ℃;
(5) artificial insemination: 20-28 hours after induced spawning, extruding egg grains from female fish, mixing sperm and eggs, and performing dry insemination to obtain fertilized eggs;
(6) hatching: carrying out oxygen-charging incubation in clean water at the water temperature of 18-23 ℃, building a sunshade net above the incubation pool for shading treatment, keeping dissolved oxygen in the water at more than 5mg/L, and removing dead eggs every day during incubation; injecting and changing water once a day, injecting and changing water 2-3 times a day after 3 days, and placing the fries in a cement pond for cultivation after the fries are gradually black; and (4) the accumulated temperature of rhodeus in the incubation period needs 36-45 days, and the fries of spodoptera spongiensis are obtained after 13-15 days.
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CN104012435B (en) * | 2014-06-13 | 2015-12-30 | 临沂大学 | China Bitterling Welfare (Rhodeus sinensis) efficient propagation method |
CN104012437B (en) * | 2014-06-19 | 2016-02-24 | 中国水产科学研究院淡水渔业研究中心 | China Bitterling Welfare artificial fecundation method |
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