CN106069340A - A kind of method for culturing seedlings of Fructus Vitis viniferae - Google Patents
A kind of method for culturing seedlings of Fructus Vitis viniferae Download PDFInfo
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- CN106069340A CN106069340A CN201610498036.6A CN201610498036A CN106069340A CN 106069340 A CN106069340 A CN 106069340A CN 201610498036 A CN201610498036 A CN 201610498036A CN 106069340 A CN106069340 A CN 106069340A
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- Prior art keywords
- seed
- vitis viniferae
- fructus vitis
- sootiness
- seedling
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
Abstract
The invention provides the method for culturing seedlings of a kind of Fructus Vitis viniferae, concrete steps include: seed selection, disinfect, Cotton seeds, constant temperature accelerating germination, and sootiness processes, sowing, seedling management；The method using the present invention, can not only improve the germination percentage of seed of Fructus Vitis viniferae, shortens the sprout time of seed, promotes growth of seedling, and seedling is healthy and strong, grows fine, the most indirectly improves the yield of Fructus Vitis viniferae, also reduce the pest and disease damage frequency of Fructus Vitis viniferae after cultivation.
Description
Technical field
The invention belongs to field of agricultural cultivation, particularly relate to the method for culturing seedlings of a kind of Fructus Vitis viniferae.
Background technology
Grape fruit is nutritious, do not contain only glucose, fructose, multiple organic acid, mineral and 20 several amino acids,
Aromatic substance, cellulose etc..Research in recent years shows, Fructus Vitis viniferae contains resveratrol and multivitamin, to anti-curing cancers and painstaking effort
Pipe disease has good effect.Fructus Vitis viniferae become it has been recognized that important health fruit.Fructus Vitis viniferae is had many uses, except fruit can be fresh
Eat, make wine, juice processed, system are dry, outside canned food processed, it is also possible to process Fructus Vitis viniferae pulp and Fructus Vitis viniferae fruit jelly.And the cultivating seeds side of present stage
Method, the time of cultivating is long, and emergence rate and the survival rate of Fructus Vitis viniferae are low, therefore, along with the large-area popularization of Fructus Vitis viniferae, in the urgent need to research and development one
Kind can large-scale promotion, affected by environment little, cultivate that speed is fast and seed seedling-raising method that survival rate is high, in order to meet Portugal
Grape demand to seedling during spread.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide the method for culturing seedlings of a kind of Fructus Vitis viniferae.
The method for culturing seedlings of a kind of Fructus Vitis viniferae, specifically includes following steps:
(1) seed selection: be placed in large beaker by seed and add distilled water immersion 5-10min, then by Glass rod continuous stirring 3-5min
Stop, liquid surface static 5-20min by the time, remove the seed floated on the surface, the seed sinking to beaker bottom is taken out, obtains nursery
Seed；
(2) disinfect: wet concentration seed is fully immersed in the HgCl of mass concentration 0.1%23-5min in reagent, takes out, and uses
Distilled water rinses 3-5 time to obtain disinfection seed repeatedly；
(3) Cotton seeds: the seed after sterilized is put in the coating agent solutions prepared, is then placed in calorstat and contaminates
Color, the temperature of calorstat is set to 30-35 DEG C, stirs 1-2min, time 4-5h every 3-4h Glass rod in dyeing course；
(4) constant temperature accelerating germination: after the seed of Fructus Vitis viniferae after rubbing process mixes with river sand, put in the refrigerator of 4-5 DEG C in cold preservation
The mass ratio of 10-12h, seed of Fructus Vitis viniferae and river sand is 1:3, then takes out, and is placed in 20-25 DEG C of calorstat, accelerating germination 24-36h, urges
Keep the water content of seed and river sand during bud between 40-54%；
(5) sootiness processes: seed of Fructus Vitis viniferae after constant temperature accelerating germination and river sand being spread on the plank frame fire-cureed in room, spreading thickness is
6-8cm, lays sootiness material around the room that fire-cures, lights Caulis et Folium Oryzae, make sootiness material be in the state of lighting but the flames of anger, be in and emit
Cigarette state, sootiness seed, is dark brown to covering of a seed；
(6) sowing: seed of Fructus Vitis viniferae sootiness processed is seeded in substrate, and nursery temperature controls at 25-28 DEG C, and illumination is strong
Degree 800-1000Lux, illumination every day 8h, in incubation, culture plate keeps moistening, until germination, grows seedling；
(7) seedling management: daytime, nursery temperature controlled at 25-30 DEG C, air humidity controls as 75-77%, illumination 8-10h, photograph
Degree is 1200-1500Lux；Night, nursery temperature controlled at 22-26 DEG C, and air humidity controls to be 75-77%, and ambient temperature is 20-
25 DEG C, the water content keeping substrate is 40-55%；When seedling grows to 5-6cm, transplant.
Described sootiness material is all with dry weight, is made up of the raw material of following weight portion: Fructus Capsici 12-16 part, Fructus Cumini Cymini
8-12 part, plant haulm end 10-12 part, Folium Artemisiae Argyi 3-5 part.
Described substrate is made up of the raw material of following weight portion: Kaolin 30-34 part, kieselguhr 20-24 part, Vermiculitum 10-
12 parts, cotton seeds shell 4-7 part, Pericarppium arachidis hypogaeae 3-6 part, wood flour 6-8 part, Sargassum powder 3-4 part, EM bacterium 1-2 part, water-retaining agent 1-3 part.
The preparation process of described Fructus Vitis viniferae seedling medium:
(1) being mixed with kieselguhr by Kaolin, adding mass concentration standby for mixed weight 5-7 is 2-3% sodium hydroxide solution, weight
Living and be heated to boiling, naturally cool to room temperature, filter, being dried to water content is 20%, pulverizes, and crosses 80-100 mesh sieve, standby；
(2) by Vermiculitum after 250-300 DEG C of temperature lower calcination 30min, being cooled to room temperature, being crushed to particle diameter is 30-40 μm, standby
With；
(3) by cotton seeds shell and Pericarppium arachidis hypogaeae, pulverize, cross 80-100 mesh sieve, then to flower seed shell and Pericarppium arachidis hypogaeae surface sprinkling
Clear water, the most moistening to cotton seeds shell and Pericarppium arachidis hypogaeae surface, finally implant EM bacterium sealing and fermenting 20-25 days, standby；
(4) raw material in step (1), (2) and (3) is homogenously mixed together, more uniformly mixes with wood flour, Sargassum powder, water-retaining agent
It is combined together, sprays the clear water of mixing gross weight 0.8-1.5 times, sealing and fermenting 8-10 days, at interval of turning in 3 days 1 time, seal and send out
After ferment, obtain Fructus Vitis viniferae seedling medium.
The invention has the beneficial effects as follows: compared with existing Fructus Vitis viniferae seedling-raising technique, have the Seedling time short, survival rate of seedling is high,
Yield is high, the advantage of quality better；The method using the present invention, uses distilled water to choose seeds, and the seed selected is full, disease-free
Fine quality；Seed is coated agent solution soak, improves the emergence rate of seed, contribute to Fructus Vitis viniferae growth in the future,
Enhance the resistance against diseases of Fructus Vitis viniferae；Use constant temperature and sootiness to process, it is simple to the softening of Fructus Vitis viniferae seed coat, accelerate the water suction speed of seed
Degree, and beneficially starch, the material such as protein is converted into solable matter and absorbs for embryo, shortens the sprout time of seed, raw
The seedling grown is healthy and strong, grows fine, the most indirectly improve the yield of Fructus Vitis viniferae after cultivation, and the pest and disease damage also reducing Fructus Vitis viniferae occurs
Number of times, improves emergence rate；The Fructus Vitis viniferae seedling medium used carries out nursery, can not only improve its survival rate, additionally it is possible to promote seedling
G and D, the Kaolin after being processed by interpolation and kieselguhr mixture, enhance the ability of substrate fertilizer conservation moisturizing, make
Substrate is loose, permeability is good, absorbability strengthens, it is possible to increase the germination percentage of seed of Fructus Vitis viniferae, and germination percentage reaches 93.6%, moves
Survival rate after cultivation, to more than 98%, also promotes the growth of seedling.
Detailed description of the invention
Embodiment 1
The method for culturing seedlings of a kind of Fructus Vitis viniferae, specifically includes following steps:
(1) seed selection: be placed in large beaker by seed and add distilled water immersion 5-10min, then by Glass rod continuous stirring 3-5min
Stop, liquid surface static 5-20min by the time, remove the seed floated on the surface, the seed sinking to beaker bottom is taken out, obtains nursery
Seed；
(2) disinfect: wet concentration seed is fully immersed in the HgCl of mass concentration 0.1%23-5min in reagent, takes out, and uses
Distilled water rinses 3-5 time to obtain disinfection seed repeatedly；
(3) Cotton seeds: the seed after sterilized is put in the coating agent solutions prepared, is then placed in calorstat and contaminates
Color, the temperature of calorstat is set to 30-35 DEG C, stirs 1-2min, time 4-5h every 3-4h Glass rod in dyeing course；
(4) constant temperature accelerating germination: after the seed of Fructus Vitis viniferae after rubbing process mixes with river sand, put in the refrigerator of 4-5 DEG C in cold preservation
The mass ratio of 10-12h, seed of Fructus Vitis viniferae and river sand is 1:3, then takes out, and is placed in 20-25 DEG C of calorstat, accelerating germination 24-36h, urges
Keep the water content of seed and river sand during bud between 40-54%；
(5) sootiness processes: seed of Fructus Vitis viniferae after constant temperature accelerating germination and river sand being spread on the plank frame fire-cureed in room, spreading thickness is
6-8cm, lays sootiness material around the room that fire-cures, lights Caulis et Folium Oryzae, make sootiness material be in the state of lighting but the flames of anger, be in and emit
Cigarette state, sootiness seed, is dark brown to covering of a seed；
(6) sowing: seed of Fructus Vitis viniferae sootiness processed is seeded in substrate, and nursery temperature controls at 25-28 DEG C, and illumination is strong
Degree 800-1000Lux, illumination every day 8h, in incubation, culture plate keeps moistening, until germination, grows seedling；
(7) seedling management: daytime, nursery temperature controlled at 25-30 DEG C, air humidity controls as 75-77%, illumination 8-10h, photograph
Degree is 1200-1500Lux；Night, nursery temperature controlled at 22-26 DEG C, and air humidity controls to be 75-77%, and ambient temperature is 20-
25 DEG C, the water content keeping substrate is 40-55%；When seedling grows to 5-6cm, transplant.
Described sootiness material is all with dry weight, is made up of the raw material of following weight portion: 16 parts of Fructus Capsici, Fructus Cumini Cymini 8 parts,
Plant haulm end 12 parts, Folium Artemisiae Argyi 3 parts.
Described substrate is made up of the raw material of following weight portion: Kaolin 34 parts, 24 parts of kieselguhr, Vermiculitum 12 parts, Cotton Gossypii
Seed hulls 7 parts, Pericarppium arachidis hypogaeae 6 parts, wood flour 6 parts, Sargassum powder 3 parts, EM bacterium 2 parts, water-retaining agent 3 parts.
The preparation process of described Fructus Vitis viniferae seedling medium:
(1) being mixed with kieselguhr by Kaolin, adding mass concentration standby for mixed weight 5-7 is 2-3% sodium hydroxide solution, weight
Living and be heated to boiling, naturally cool to room temperature, filter, being dried to water content is 20%, pulverizes, and crosses 80-100 mesh sieve, standby；
(2) by Vermiculitum after 250-300 DEG C of temperature lower calcination 30min, being cooled to room temperature, being crushed to particle diameter is 30-40 μm, standby
With；
(3) by cotton seeds shell and Pericarppium arachidis hypogaeae, pulverize, cross 80-100 mesh sieve, then to flower seed shell and Pericarppium arachidis hypogaeae surface sprinkling
Clear water, the most moistening to cotton seeds shell and Pericarppium arachidis hypogaeae surface, finally implant EM bacterium sealing and fermenting 20-25 days, standby；
(4) raw material in step (1), (2) and (3) is homogenously mixed together, more uniformly mixes with wood flour, Sargassum powder, water-retaining agent
It is combined together, sprays the clear water of mixing gross weight 0.8-1.5 times, sealing and fermenting 8-10 days, at interval of turning in 3 days 1 time, seal and send out
After ferment, obtain Fructus Vitis viniferae seedling medium.
Embodiment 2
Embodiment 2 difference from Example 1 is in sootiness material and substrate that the parts by weight of raw material are different, and other are equal
Identical.
Described sootiness material is made up of the raw material of following weight portion: 12 parts of Fructus Capsici, Fructus Cumini Cymini 8 parts, plant haulm end 12 parts,
Folium Artemisiae Argyi 5 parts.
Described substrate is made up of the raw material of following weight portion: Kaolin 34 parts, 24 parts of kieselguhr, Vermiculitum 12 parts, Cotton Gossypii
Seed hulls 4 parts, Pericarppium arachidis hypogaeae 3 parts, wood flour 6 parts, Sargassum powder 3 parts, EM bacterium 1 part, water-retaining agent 3 parts.
Embodiment 3
Embodiment 3 difference from Example 1 is in sootiness material and substrate that the parts by weight of raw material are different, and other are equal
Identical.
Described sootiness material is made up of the raw material of following weight portion: 16 parts of Fructus Capsici, Fructus Cumini Cymini 12 parts, plant haulm end 12
Part, Folium Artemisiae Argyi 5 parts.
Described substrate is made up of the raw material of following weight portion: Kaolin 34 parts, 24 parts of kieselguhr, Vermiculitum 12 parts, Cotton Gossypii
Seed hulls 7 parts, Pericarppium arachidis hypogaeae 6 parts, wood flour 8 parts, Sargassum powder 4 parts, EM bacterium 2 parts, water-retaining agent 3 parts.
Claims (4)
1. the method for culturing seedlings of a Fructus Vitis viniferae, it is characterised in that specifically include following steps:
(1) seed selection: be placed in large beaker by seed and add distilled water immersion 5-10min, then by Glass rod continuous stirring 3-5min
Stop, liquid surface static 5-20min by the time, remove the seed floated on the surface, the seed sinking to beaker bottom is taken out, obtains nursery
Seed；
(2) disinfect: wet concentration seed is fully immersed in the HgCl of mass concentration 0.1%23-5min in reagent, takes out, with steaming
Distilled water rinses 3-5 time to obtain disinfection seed repeatedly；
(3) Cotton seeds: the seed after sterilized is put in the coating agent solutions prepared, is then placed in calorstat and contaminates
Color, the temperature of calorstat is set to 30-35 DEG C, stirs 1-2min, time 4-5h every 3-4h Glass rod in dyeing course；
(4) constant temperature accelerating germination: after the seed of Fructus Vitis viniferae after rubbing process mixes with river sand, put in the refrigerator of 4-5 DEG C in cold preservation
The mass ratio of 10-12h, seed of Fructus Vitis viniferae and river sand is 1:3, then takes out, and is placed in 20-25 DEG C of calorstat, accelerating germination 24-36h, urges
Keep the water content of seed and river sand during bud between 40-54%；
(5) sootiness processes: seed of Fructus Vitis viniferae after constant temperature accelerating germination and river sand being spread on the plank frame fire-cureed in room, spreading thickness is
6-8cm, lays sootiness material around the room that fire-cures, lights Caulis et Folium Oryzae, make sootiness material be in the state of lighting but the flames of anger, be in and emit
Cigarette state, sootiness seed, is dark brown to covering of a seed；
(6) sowing: seed of Fructus Vitis viniferae sootiness processed is seeded in substrate, and nursery temperature controls at 25-28 DEG C, and illumination is strong
Degree 800-1000Lux, illumination every day 8h, in incubation, culture plate keeps moistening, until germination, grows seedling；
(7) seedling management: daytime, nursery temperature controlled at 25-30 DEG C, air humidity controls as 75-77%, illumination 8-10h, photograph
Degree is 1200-1500Lux；Night, nursery temperature controlled at 22-26 DEG C, and air humidity controls to be 75-77%, and ambient temperature is 20-
25 DEG C, the water content keeping substrate is 40-55%；When seedling grows to 5-6cm, transplant.
The method for culturing seedlings of Fructus Vitis viniferae the most according to claim 1, it is characterised in that described sootiness material is all with dry weight
Meter, is made up of the raw material of following weight portion: Fructus Capsici 12-16 part, Fructus Cumini Cymini 8-12 part, plant haulm end 10-12 part, Folium Artemisiae Argyi 3-5 part.
The method for culturing seedlings of Fructus Vitis viniferae kind the most according to claim 1, it is characterised in that described substrate is by following weight portion
Raw material is made: Kaolin 30-34 part, kieselguhr 20-24 part, Vermiculitum 10-12 part, cotton seeds shell 4-7 part, Pericarppium arachidis hypogaeae 3-6 part,
Wood flour 6-8 part, Sargassum powder 3-4 part, EM bacterium 1-2 part, water-retaining agent 1-3 part.
The method for culturing seedlings of Fructus Vitis viniferae kind the most according to claim 1, it is characterised in that the preparation of described Fructus Vitis viniferae seedling medium
Step:
(1) being mixed with kieselguhr by Kaolin, adding mass concentration standby for mixed weight 5-7 is 2-3% sodium hydroxide solution, weight
Living and be heated to boiling, naturally cool to room temperature, filter, being dried to water content is 20%, pulverizes, and crosses 80-100 mesh sieve, standby；
(2) by Vermiculitum after 250-300 DEG C of temperature lower calcination 30min, being cooled to room temperature, being crushed to particle diameter is 30-40 μm, standby
With；
(3) by cotton seeds shell and Pericarppium arachidis hypogaeae, pulverize, cross 80-100 mesh sieve, then to flower seed shell and Pericarppium arachidis hypogaeae surface sprinkling
Clear water, the most moistening to cotton seeds shell and Pericarppium arachidis hypogaeae surface, finally implant EM bacterium sealing and fermenting 20-25 days, standby；
(4) raw material in step (1), (2) and (3) is homogenously mixed together, more uniformly mixes with wood flour, Sargassum powder, water-retaining agent
It is combined together, sprays the clear water of mixing gross weight 0.8-1.5 times, sealing and fermenting 8-10 days, at interval of turning in 3 days 1 time, seal and send out
After ferment, obtain Fructus Vitis viniferae seedling medium.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107135876A (en) * | 2017-05-07 | 2017-09-08 | 韦昌仕 | A kind of Piao Shu seeding growing seedlings methods |
| CN107216176A (en) * | 2017-06-29 | 2017-09-29 | 徐州两汉农业发展有限公司 | A kind of culture matrix for improving grape seed germination percentage and preparation method thereof |
| CN108064679A (en) * | 2017-12-23 | 2018-05-25 | 全椒县潘林园林葡萄专业合作社 | A kind of rapid propagation method of grape seedling |
| CN108432601A (en) * | 2018-03-20 | 2018-08-24 | 广西长耀隆农业科技有限公司 | A kind of grape seedling medium and preparation method thereof |
| CN108617428A (en) * | 2018-05-11 | 2018-10-09 | 贵州务川国韵生态农业有限公司 | A kind of cultural method of wormwood |
| CN109380054A (en) * | 2018-12-02 | 2019-02-26 | 云南省农业科学院热区生态农业研究所 | A kind of new method of Hybrid Grape seed seedling-raising |
| CN112544333A (en) * | 2020-11-24 | 2021-03-26 | 山西省农业科学院果树研究所 | Drought-resistant cultivation method for grapes |
| CN116584293A (en) * | 2023-03-16 | 2023-08-15 | 中国长江三峡集团有限公司 | Method for efficiently breeding eucommia ulmoides in low-altitude area |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107135876A (en) * | 2017-05-07 | 2017-09-08 | 韦昌仕 | A kind of Piao Shu seeding growing seedlings methods |
| CN107216176A (en) * | 2017-06-29 | 2017-09-29 | 徐州两汉农业发展有限公司 | A kind of culture matrix for improving grape seed germination percentage and preparation method thereof |
| CN108064679A (en) * | 2017-12-23 | 2018-05-25 | 全椒县潘林园林葡萄专业合作社 | A kind of rapid propagation method of grape seedling |
| CN108432601A (en) * | 2018-03-20 | 2018-08-24 | 广西长耀隆农业科技有限公司 | A kind of grape seedling medium and preparation method thereof |
| CN108617428A (en) * | 2018-05-11 | 2018-10-09 | 贵州务川国韵生态农业有限公司 | A kind of cultural method of wormwood |
| CN109380054A (en) * | 2018-12-02 | 2019-02-26 | 云南省农业科学院热区生态农业研究所 | A kind of new method of Hybrid Grape seed seedling-raising |
| CN112544333A (en) * | 2020-11-24 | 2021-03-26 | 山西省农业科学院果树研究所 | Drought-resistant cultivation method for grapes |
| CN116584293A (en) * | 2023-03-16 | 2023-08-15 | 中国长江三峡集团有限公司 | Method for efficiently breeding eucommia ulmoides in low-altitude area |
| CN116584293B (en) * | 2023-03-16 | 2024-04-02 | 中国长江三峡集团有限公司 | Method for efficiently breeding eucommia ulmoides in low-altitude area |
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Application publication date: 20161109 |