CN107567965A - A kind of method of daphne odera seed seeding and seedling raising - Google Patents
A kind of method of daphne odera seed seeding and seedling raising Download PDFInfo
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- CN107567965A CN107567965A CN201710886498.XA CN201710886498A CN107567965A CN 107567965 A CN107567965 A CN 107567965A CN 201710886498 A CN201710886498 A CN 201710886498A CN 107567965 A CN107567965 A CN 107567965A
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- nutritive cream
- daphne odera
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Abstract
The present invention discloses a kind of method of daphne odera seed seeding and seedling raising, including following operating procedure：（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, it is mixed with single semina；（2）Mixed seed is put into germination liquid abundant immersion treatment 23 hours；（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniformly sprinkling upon in nutritive cream；（4）Culture dish is put into incubator, it is 27 31 DEG C to keep the temperature in case, daily photo-irradiation treatment 11 13 hours.The method of daphne odera seed seeding and seedling raising provided by the invention, simple to operate, cost is cheap, the germination rate of daphne odera seed can be effectively lifted, and the growing way of seedling is excellent, and the survival rate of seedling is high, the efficiency of daphne odera seeding and seedling raising is greatly improved, improves economic benefit.
Description
Technical field
The invention belongs to daphne odera planting technology field, a kind of method of daphne odera seed seeding and seedling raising of specific design.
Background technology
Daphne odera is evergreen shrubs, can also grow up to dungarunga sample sometimes, annual shoot, young leaves rachis and bennet are by sparse micro-
Pubescence, become thereafter hairless.Daphne odera requires less tight to soil, more drought-enduring.The temperature of daphne odera optimum growth is 26 DEG C of left sides
The right side, less cold-resistant, if temperature is less than 0 DEG C, daphne odera understands frost bitten, or even freezes to death;If surviving the winter indoors, room temperature can not be too high,
Otherwise its dormancy can be influenceed, consumes excessive nutrition in plant body, it is unfavorable that next year is grown, and can influence its growth then.
Daphne odera is heliophilous species, and at least 6h or so direct lights shine daily, can the flourishing fragrance of a flower, therefore sunny, air stream
Conveniently its growth of logical place.Daphne odera is more using white right seed propagation, and usual germination rate is relatively low, the storage before Seed sprouting
Number of days is more, complete germination process when ask length, dormancy be present in Some seeds.Therefore, when difficult emergence, emergence in production be present
Ask long and emerge phenomena such as irregular.
The content of the invention
In order to solve the above-mentioned technical problem, the present invention provides a kind of method of daphne odera seed seeding and seedling raising.
The present invention is achieved by the following technical solutions.
A kind of method of daphne odera seed seeding and seedling raising, including following operating procedure：
（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, by it
Mixed with single semina；
（2）Mixed seed is put into germination liquid abundant immersion treatment 2-3 hours, wherein germination liquid is by following parts by weight
Component is made：Barbiturates 2-5 parts, sodium tert-butoxide 6-8 parts, sodium borohydride 3-8 parts, indolebutyric acid 1-2 parts, water 900-1000
Part；
（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniform
Be sprinkling upon in nutritive cream, wherein nutritive cream is made up of the component of following parts by weight：Linseed 14-20 parts, semen momordicae 10-15 parts,
Lepidium seed 9-11 parts, seed of feather cockscomb 6-10 parts, common bombax flower 3-7 parts, Chinese mesona herb 16-20 parts, agar 30-40 parts, water 1000-1200
Part；
（4）Culture dish is put into incubator, it is 27-31 DEG C to keep the temperature in case, daily photo-irradiation treatment 11-13 hours.
Specifically, above-mentioned steps（2）The mass volume ratio of middle seed and soak is 1kg:2-4L.
Specifically, above-mentioned steps（3）Middle nutritive cream is made of following methods：By linseed, semen momordicae, lepidium seed, green grass or young crops
After feather cockscomb, common bombax flower, Chinese mesona herb dry, grind into powder, then 75 DEG C are heated water to, agar is added thereto, is mixed
After uniformly, after continuation adds the powder of above-mentioned gained thereto, 10min is persistently stirred using 300r/min rotating speed, is worth battalion
Support cream.
Specifically, above-mentioned steps（3）In, the height after nutritive cream caking in culture dish is 1-1.5cm.
Technical scheme more than, the beneficial effects of the invention are as follows：
The method of daphne odera seed seeding and seedling raising provided by the invention, simple to operate, cost is cheap, can effectively lift daphne odera
The germination rate of seed, and the growing way of seedling is excellent, and the survival rate of seedling is high, greatly improves the effect of daphne odera seeding and seedling raising
Rate, improve economic benefit.Wherein, due to the special tectonic of double seminas, nutriment skewness in seed, difficult quilt
Absorb, after sodium vanadate solution immersion treatment, can effectively lift seed and effect is utilized to its endotrophic material
Rate, and then improve the germination percentage of seed；Germination liquid provided by the invention, after barbiturates, sodium tert-butoxide act synergistically, can have
The activity for reducing sprout inhibition material in seed of effect, after sodium borohydride, indolebutyric acid act synergistically, it can effectively lift seed
The vigor of interior enzyme, and the seed germination cycle can be regulated and controled, and then the effective germination percentage for improving daphne odera seed；The present invention carries
High nutritive cream, substantial amounts of nutriment can be provided for seed, it is ensured that seed is after sprouting during the sprouting of seed
Very long a period of time in, have the nutrition supply of abundance, and then ensure that the growing way of seedling；Meanwhile offer of the invention
Nutritive cream, can effectively avoid seed during sprouting, the phenomenon gone mouldy.
Embodiment
Following examples are used to illustrate the present invention, but can not be used for limiting the scope of the present invention.The reality used in embodiment
The condition of applying can be for further adjustments according to the condition of producer, and unaccounted implementation condition is usually conventional laboratory conditions.
Embodiment 1
A kind of method of daphne odera seed seeding and seedling raising, including following operating procedure：
（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, by it
Mixed with single semina；
（2）Mixed seed is put into germination liquid abundant immersion treatment 2 hours, wherein germination liquid by following parts by weight group
Divide and be made：2 parts of barbiturates, 6 parts of sodium tert-butoxide, 3 parts of sodium borohydride, 1 part of indolebutyric acid, 900 parts of water；
（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniform
Be sprinkling upon in nutritive cream, wherein nutritive cream is made up of the component of following parts by weight：14 parts of linseed, 10 parts of semen momordicae, lepidium seed 9
Part, 6 parts of the seed of feather cockscomb, 3 parts of common bombax flower, 16 parts of Chinese mesona herb, 30 parts of agar, 1000 parts of water；
（4）Culture dish is put into incubator, it is 27 DEG C to keep the temperature in case, daily photo-irradiation treatment 11 hours.
Specifically, above-mentioned steps（2）The mass volume ratio of middle seed and soak is 1kg:2L.
Specifically, above-mentioned steps（3）Middle nutritive cream is made of following methods：By linseed, semen momordicae, lepidium seed, green grass or young crops
After feather cockscomb, common bombax flower, Chinese mesona herb dry, grind into powder, then 75 DEG C are heated water to, agar is added thereto, is mixed
After uniformly, after continuation adds the powder of above-mentioned gained thereto, 10min is persistently stirred using 300r/min rotating speed, is worth battalion
Support cream.
Specifically, above-mentioned steps（3）In, the height after nutritive cream caking in culture dish is 1cm.
Embodiment 2
A kind of method of daphne odera seed seeding and seedling raising, including following operating procedure：
（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, by it
Mixed with single semina；
（2）Mixed seed is put into germination liquid abundant immersion treatment 2.5 hours, wherein germination liquid is by following parts by weight
Component is made：3 parts of barbiturates, 7 parts of sodium tert-butoxide, 5 parts of sodium borohydride, 1 part of indolebutyric acid, 950 parts of water；
（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniform
Be sprinkling upon in nutritive cream, wherein nutritive cream is made up of the component of following parts by weight：16 parts of linseed, 13 parts of semen momordicae, lepidium seed
10 parts, 8 parts of the seed of feather cockscomb, 5 parts of common bombax flower, 18 parts of Chinese mesona herb, 35 parts of agar, 1100 parts of water；
（4）Culture dish is put into incubator, it is 28 DEG C to keep the temperature in case, daily photo-irradiation treatment 12 hours.
Specifically, above-mentioned steps（2）The mass volume ratio of middle seed and soak is 1kg:3L.
Specifically, above-mentioned steps（3）Middle nutritive cream is made of following methods：By linseed, semen momordicae, lepidium seed, green grass or young crops
After feather cockscomb, common bombax flower, Chinese mesona herb dry, grind into powder, then 75 DEG C are heated water to, agar is added thereto, is mixed
After uniformly, after continuation adds the powder of above-mentioned gained thereto, 10min is persistently stirred using 300r/min rotating speed, is worth battalion
Support cream.
Specifically, above-mentioned steps（3）In, the height after nutritive cream caking in culture dish is 1.3cm.
Embodiment 3
A kind of method of daphne odera seed seeding and seedling raising, including following operating procedure：
（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, by it
Mixed with single semina；
（2）Mixed seed is put into germination liquid abundant immersion treatment 3 hours, wherein germination liquid by following parts by weight group
Divide and be made：5 parts of barbiturates, 8 parts of sodium tert-butoxide, 8 parts of sodium borohydride, 2 parts of indolebutyric acid, 1000 parts of water；
（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniform
Be sprinkling upon in nutritive cream, wherein nutritive cream is made up of the component of following parts by weight：20 parts of linseed, 15 parts of semen momordicae, lepidium seed
11 parts, 10 parts of the seed of feather cockscomb, 7 parts of common bombax flower, 20 parts of Chinese mesona herb, 40 parts of agar, 1200 parts of water；
（4）Culture dish is put into incubator, it is 31 DEG C to keep the temperature in case, daily photo-irradiation treatment 13 hours.
Specifically, above-mentioned steps（2）The mass volume ratio of middle seed and soak is 1kg:4L.
Specifically, above-mentioned steps（3）Middle nutritive cream is made of following methods：By linseed, semen momordicae, lepidium seed, green grass or young crops
After feather cockscomb, common bombax flower, Chinese mesona herb dry, grind into powder, then 75 DEG C are heated water to, agar is added thereto, is mixed
After uniformly, after continuation adds the powder of above-mentioned gained thereto, 10min is persistently stirred using 300r/min rotating speed, is worth battalion
Support cream.
Specifically, above-mentioned steps（3）In, the height after nutritive cream caking in culture dish is 1.5cm.
Comparative example 1
Without step（1）Method processing, remaining operating procedure is identical with embodiment 1.
Comparative example 2
Barbiturates, sodium tert-butoxide, sodium borohydride composition are not contained in germination liquid, remaining operating procedure and embodiment 2 are complete
It is identical.
Comparative example 3
Nutritive cream is not added in culture dish, remaining operating procedure is identical with embodiment 3.
Respectively with the method for each embodiment and comparative example to carrying out seeding and seedling raising experiment, every group of examination with a collection of daphne odera seed
The number seeds tested are 1000, and the time of experiment is 30, and result of the test is as shown in table 1：
The situation of the daphne odera seed development of table 1
| Project | Germination rate, % | Seed goes mouldy grain number, grain | Newborn spray length |
| Embodiment 1 | 87 | 6 | 6.4 |
| Comparative example 1 | 69 | 6 | 5.5 |
| Embodiment 2 | 88 | 5 | 6.7 |
| Comparative example 2 | 62 | 8 | 4.1 |
| Embodiment 3 | 88 | 4 | 6.8 |
| Comparative example 3 | 55 | 53 | 3.6 |
As shown in Table 1, the method for a kind of daphne odera seed seeding and seedling raising provided by the invention, can effectively lift the germination of seed
Rate, reduces the number that goes mouldy of seed, and can carry and make it that the growing way of shoot is fine.
Certainly, it is limitation of the present invention that described above, which is not, and the present invention is also not limited to the example above, the art
Those of ordinary skill, the present invention essential scope in, change, change, addition or the replacement made, should all belong to the present invention
Protection domain.
Claims (4)
- A kind of 1. method of daphne odera seed seeding and seedling raising, it is characterised in that including following operating procedure：（1）Double semina are screened, are put into the sodium vanadate solution of mass fraction 3% after immersion treatment 25min, by it Mixed with single semina；（2）Mixed seed is put into germination liquid abundant immersion treatment 2-3 hours, wherein germination liquid is by following parts by weight Component is made：Barbiturates 2-5 parts, sodium tert-butoxide 6-8 parts, sodium borohydride 3-8 parts, indolebutyric acid 1-2 parts, water 900-1000 Part；（3）Nutritive cream is uniformly poured on culture dish, after nutritive cream caking, step will be passed through（2）The seed of processing is uniform Be sprinkling upon in nutritive cream, wherein nutritive cream is made up of the component of following parts by weight：Linseed 14-20 parts, semen momordicae 10-15 parts, Lepidium seed 9-11 parts, seed of feather cockscomb 6-10 parts, common bombax flower 3-7 parts, Chinese mesona herb 16-20 parts, agar 30-40 parts, water 1000-1200 Part；（4）Culture dish is put into incubator, it is 27-31 DEG C to keep the temperature in case, daily photo-irradiation treatment 11-13 hours.
- 2. according to a kind of method of daphne odera seed seeding and seedling raising described in claim 1, it is characterised in that above-mentioned steps （2）The mass volume ratio of middle seed and soak is 1kg:2-4L.
- 3. according to a kind of method of daphne odera seed seeding and seedling raising described in claim 1, it is characterised in that above-mentioned steps （3）Middle nutritive cream is made of following methods：Linseed, semen momordicae, lepidium seed, the seed of feather cockscomb, common bombax flower, Chinese mesona herb are dried Afterwards, grind into powder, then 75 DEG C are heated water to, agar is added thereto, after being mixed evenly, continues to add thereto After the powder for stating gained, 10min is persistently stirred using 300r/min rotating speed, is worth nutritive cream.
- 4. according to a kind of method of daphne odera seed seeding and seedling raising described in claim 1, it is characterised in that above-mentioned steps （3）In, the height after nutritive cream caking in culture dish is 1-1.5cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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| CN106471931A (en) * | 2016-09-29 | 2017-03-08 | 张建委 | A kind of implantation methods of organic hot pepper |
| CN106561427A (en) * | 2016-10-25 | 2017-04-19 | 王伟 | Capsicum seedling growing method being suitable for the north of Anhui Province |
| CN107135760A (en) * | 2017-04-05 | 2017-09-08 | 安徽咱家田生态农业有限公司 | A kind of Snakegourd Fruit seeding growing seedlings method |
| CN107182660A (en) * | 2017-05-10 | 2017-09-22 | 蚌埠王恒亮盆景艺术有限公司 | A kind of method of cypress high-survival rate grafting |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106471931A (en) * | 2016-09-29 | 2017-03-08 | 张建委 | A kind of implantation methods of organic hot pepper |
| CN106561427A (en) * | 2016-10-25 | 2017-04-19 | 王伟 | Capsicum seedling growing method being suitable for the north of Anhui Province |
| CN107135760A (en) * | 2017-04-05 | 2017-09-08 | 安徽咱家田生态农业有限公司 | A kind of Snakegourd Fruit seeding growing seedlings method |
| CN107182660A (en) * | 2017-05-10 | 2017-09-22 | 蚌埠王恒亮盆景艺术有限公司 | A kind of method of cypress high-survival rate grafting |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113748775A (en) * | 2021-08-05 | 2021-12-07 | 广东省农业科学院作物研究所 | Method for promoting germination of murraya paniculata seeds |
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