CN114847161B - Method for improving transplanting survival rate and fragrance of cymbidium She Zupei seedlings - Google Patents

Method for improving transplanting survival rate and fragrance of cymbidium She Zupei seedlings Download PDF

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CN114847161B
CN114847161B CN202210514005.0A CN202210514005A CN114847161B CN 114847161 B CN114847161 B CN 114847161B CN 202210514005 A CN202210514005 A CN 202210514005A CN 114847161 B CN114847161 B CN 114847161B
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seedling
cymbidium
zupei
seedlings
culture
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CN114847161A (en
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吉训志
秦晓威
鱼欢
张昂
贺书珍
宗迎
初众
唐冰
郝朝运
闫林
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Spice and Beverage Research Institute of Chinese Academy of Tropical Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

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Abstract

The invention provides a method for improving the transplanting survival rate and fragrance of cymbidium She Zupei seedlings, which comprises the following steps: s1) soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment; s2) transplanting the processed seedling She Zupei of the cymbidium into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70-85% humidity; s3) planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings. Compared with the prior art, the method provided by the invention can improve the transplanting survival rate of the cymbidium She Zupei seedlings, shorten the time for culturing the seedlings outside and inside the tissue culture seedling bottle, and improve the content of main aroma components of the cymbidium She Zupei seedlings, especially the content of 2-acetyl-1-pyrroline, so that the production efficiency and the seedling quality of the cymbidium leaves are effectively improved.

Description

Method for improving transplanting survival rate and fragrance of cymbidium She Zupei seedlings
Technical Field
The invention belongs to the technical field of plant breeding and culture, and particularly relates to a method for improving the transplanting survival rate and fragrance of cymbidium She Zupei seedlings.
Background
The leaves of the plant Isodon japonicus (Pandanus amaryllifolius) are known as the brand name (Pandan leaf), which is translated into the plant Isodon japonicus in China, and also known as gorgeous leaves, vanilla leaves and plant Isodon japonicus leaves, which are perennial evergreen horticultural crops of the genus Pandanaceae (Pandanus). The paphiopedilum has more than 800 kinds of plants in 3 genera, the original ecological environment of the plants in the genus is quite different, the economic uses are quite different, the paphiopedilum can be applied to the fields of medical treatment and cooking, a plurality of species can treat toothache and rheumatism in the genus, and the development and utilization prospect is wide. Wherein, the leaf of the cymbidium is a plant with unique Zongzi fragrance. The leaves of the cymbidium leaves are rich in squalene, linoleic acid, estragole, sterol and other active ingredients, have the functions of enhancing cell activity, accelerating metabolism, improving human immunity, inhibiting cancer cell growth and the like, and are known as vanilla of Orients. Besides good taste, the herba Eupatorii leaves have strong antioxidant components, and have effects of relieving summer heat, cooling and reducing pathogenic fire, tranquillizing, calming and relaxing tendons and activating collaterals, so that the herba Eupatorii leaves have very high economic development value.
The plant of the leaf of the cymbidium in China is introduced outside the country, is initially introduced into a region of the dahurian and is planted in other regions of the south of the sea, the plant is gradually developed into regions of the south of the Yunnan and the like, and the leaf of the cymbidium is planted in the provinces of Guangdong, taiwan and the like nowadays. The leaf juice of the south-east Asia is used as an additive spice to improve the quality of cakes and beverages, and is one of the food spices commonly used by people in tropical areas. The products of the cymbidium cake, the cymbidium essence, the cymbidium sauce, the cymbidium sago, the cymbidium syrup and the like which are prepared by taking the cymbidium leaves as main materials become famous special foods in tropical areas and are deeply favored by consumers. As a popular spice and tropical agricultural products with important application, the problems of vigorous demand and insufficient supply exist for a long time, and with the continuous improvement of life quality, the demand of the cymbidium leaves in China is also continuously increased. The cymbidium leaves are taken as typical tropical special spice crops, have important significance for developing local special economy and improving income and living standard of peasants in remote areas, and have wide development prospect.
The leaves of the cymbidium are mainly bred according to in the traditional planting method because flowers and fruits of the leaves are not seen. The method has at least two years of growth degree for the parent plant, and the seedlings which need tillering are limited, so that the requirements of industry on high-flux seedling breeding cannot be met, and the lack of high-flux seedling breeding technology restricts the further development of the cymbidium leaf industry. In addition, after the banlan She Zupei seedlings start to be bred in a large scale, the problems of low transplanting survival rate, more and long steps from seedling hardening in the bottle to seedling hardening outside the bottle, insufficient fragrance and the like can also occur due to growth vigor difference during transplanting.
Disclosure of Invention
In view of the above, the technical problem to be solved by the invention is to provide a method for improving the transplanting survival rate and fragrance of the seedling of the cymbidium She Zupei, which can obviously improve the transplanting survival rate of the seedling of the cymbidium She Zupei and the fragrance of the tissue culture seedling, and especially the content of 2-acetyl-1-pyrroline is obviously improved.
The invention provides a method for improving the transplanting survival rate and fragrance of cymbidium She Zupei seedlings, which comprises the following steps:
s1) soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment;
s2) transplanting the processed seedling She Zupei of the cymbidium into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70-85% humidity;
s3) planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings.
Preferably, the seedling of the cymbidium with root She Zupei is 6-10 cm high, and has complete root system and 2-6 expanded leaves.
Preferably, the concentration of the zinc sulfate solution is 30-80 mg/mL.
Preferably, the treatment time in the step S1) is 20-30 hours; keeping the blade moist during treatment; the temperature during the treatment is 25-28 ℃; the illumination intensity during treatment is 2000-4000 lx; the illumination time during the treatment is 6-12 h.
Preferably, the cultivation substrate in the step S2) is 0.5-0.8 cm of sand and stone; the seedling hardening nutrient solution is used for keeping the humidity of the culture medium at 70-85% during seedling hardening culture; the seedling hardening nutrient solution comprises 5-10 mg/L ammonium bicarbonate, 5-8 mg/L calcium superphosphate, 10-15 mg/L potassium chloride and water.
Preferably, the seedling hardening culture time in the step S2) is 20-40 days; the illumination intensity of seedling hardening culture is 2000-4000 lx; the light period of seedling hardening culture is 8-12 h of illumination time.
Preferably, the base fertilizer is applied before the planting in the step S3); the base fertilizer comprises an organic fertilizer and a nitrogen-phosphorus-potassium compound fertilizer; the mass ratio of the organic fertilizer to the nitrogen-phosphorus-potassium compound fertilizer is (500-1000): (30-50).
Preferably, the usage amount of the organic fertilizer is 500-1000 kg/666.7cm 2 。
Preferably, the temperature of the field planting is 28-35 ℃; the illumination intensity during field planting is 3000-8000 lx; the light period of field planting is 8-12 hours of illumination time; the watering amount in field planting can wet the soil by 60-80%.
The invention provides a method for improving the transplanting survival rate and fragrance of cymbidium She Zupei seedlings, which comprises the following steps: s1) soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment; s2) transplanting the processed seedling She Zupei of the cymbidium into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70-85% humidity; s3) planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings. Compared with the prior art, the method provided by the invention can improve the transplanting survival rate of the cymbidium She Zupei seedlings, shorten the time for culturing the seedlings outside and inside the tissue culture seedling bottle, and improve the content of main aroma components of the cymbidium She Zupei seedlings, especially the content of 2-acetyl-1-pyrroline, so that the production efficiency and the seedling quality of the cymbidium leaves are effectively improved.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a method for improving the transplanting survival rate and fragrance of cymbidium She Zupei seedlings, which comprises the following steps: s1) soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment; s2) transplanting the processed seedling She Zupei of the cymbidium into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70-85% humidity; s3) planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings.
The source of all the raw materials is not particularly limited, and the raw materials are commercially available.
In the invention, the height of the root-bearing cymbidium She Zupei seedlings is preferably 6-10 cm; the root-bearing cymbidium She Zupei seedlings preferably have complete root systems and 2-6 developing leaves.
Soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment; in the invention, the root culture medium of the root-bearing cymbidium She Zupei seedling is preferably washed, then the water is drained, and the root is soaked in zinc sulfate solution for treatment; the concentration of the zinc sulfate solution is preferably 30-80 mg/mL, more preferably 40-70 mg/mL, still more preferably 40-60 mg/mL, still more preferably 45-55 mg/mL, and most preferably 50mg/mL; the treatment time is preferably 20 to 30 hours, more preferably 24 to 26 hours; the method comprises the steps of carrying out a first treatment on the surface of the The illumination intensity during treatment is preferably 2000-4000 lx; the illumination time during treatment is preferably 6-12 hours; preferably, the leaves are kept moist during treatment, more preferably, the leaves of the tissue culture seedlings are kept moist by spraying water mist from a spray can; the temperature at the time of the treatment is preferably 25℃to 28 ℃.
Transplanting the processed seedling of the cymbidium She Zupei into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70-85% humidity; the culture medium is a culture medium well known to those skilled in the art, and is not particularly limited, and it is preferably 0.5 to 0.8cm of sand or stone in the present invention; the humidity of the culture medium is preferably 80% -85% during transplanting; in order to prevent the matrix from being excessively wet during seedling hardening, watering is not suitable when water exists, and preferably, when the matrix is not thoroughly dried, the seedling hardening nutrient solution keeps the humidity of the culture matrix at 70% -85%; the seedling hardening nutrient solution preferably comprises 5-10 mg/L ammonium bicarbonate, 5-8 mg/L calcium superphosphate, 10-15 mg/L potassium chloride and water, more preferably 8-10 mg/L ammonium bicarbonate, 5-6 mg/L calcium superphosphate, 10-12 mg/L potassium chloride and water, and still more preferably 10mg/L ammonium bicarbonate, 5-5 mg/L calcium superphosphate, 10mg/L potassium chloride and water; the seedling hardening nutrient solution is preferably used for the seedling hardening culture to keep the humidity of the culture medium at 70-80 percent; the seedling hardening culture time is preferably 20-40 days, more preferably 25-35 days, and still more preferably 30 days; the temperature of the seedling hardening culture is preferably 25-28 ℃; the illumination intensity is preferably 2000-4000 lx when the seedling hardening culture is performed; the light period of seedling hardening culture is preferably 8-12 hours of illumination time.
Finally, planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings; preferably applying a base fertilizer before field planting; the base fertilizer is preferably based on organic fertilizer, and more preferably comprises organic fertilizer and nitrogen, phosphorus and potassium compoundMixing fertilizer; the organic fertilizer is preferably a high-quality organic fertilizer or a commercial organic fertilizer; the nitrogen-phosphorus-potassium compound fertilizer is preferably a compound fertilizer (15-15-15); the mass preferable ratio of the organic fertilizer to the nitrogen-phosphorus-potassium compound fertilizer is (500-1000): (30-50); the usage amount of the organic fertilizer is 500-1000 kg/666.7cm 2 The method comprises the steps of carrying out a first treatment on the surface of the The field planting temperature is 28-35 ℃; the illumination intensity during field planting is 3000-8000 lx; the light period of field planting is 8-12 hours of illumination time; the soil is not excessively wet during field planting, and is watered when the soil is not dried, and the water quantity during field planting is 60% -80%, more preferably 70%.
The method provided by the invention can improve the transplanting survival rate of the cymbidium She Zupei seedlings, shorten the time for culturing the seedlings outside and inside the tissue culture seedling bottle, and can also improve the content of main aroma components of the cymbidium She Zupei seedlings, especially the content of 2-acetyl-1-pyrroline, thereby effectively improving the production efficiency of cymbidium leaves and the quality of seedlings.
In order to further illustrate the invention, the following describes in detail a method for improving the transplanting survival rate and fragrance of the cymbidium She Zupei seedlings according to the invention.
The reagents used in the examples below are all commercially available.
Example 1
Selecting 6-10 cm of cymbidium leaves with roots, soaking 2-6 leaves of tissue culture seedlings with complete root systems in a culture medium containing 50mg/ml ZnSO 4 In the liquid culture of (1 d), only the root of the seedling is soaked by using a buoy, water mist is sprayed out by using a spray can to ensure that the seedling leaves are wet, the illumination intensity during treatment is 3000lx, the illumination time is 10 hours, and the transplanting and hardening seedlings are required to be placed at a hardening temperature of 25-28 ℃; after liquid culture, transplanting the tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium to 80-85%, preventing the medium from being excessively wet, and not being suitable for watering when the medium is not thoroughly dried, and hardening seedlings by using an aqueous solution containing 10% ammonium bicarbonate, 5% superphosphate and 10% potassium chloride until the humidity is 70-80%, wherein the illumination intensity is 3000lx during hardening seedlings culture, and the light period of hardening seedlings culture is 10 hours in illumination duration; after seedling hardening and culturing for 30d, field planting, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Application ofThe organic fertilizer Norsterra is 800kg, the compound fertilizer (15-15-15) is 50kg, the seed transfer temperature is 28-35 ℃, the illumination intensity is 5000lx, the photoperiod is 10 hours of illumination duration, the land is not too wet, watering is carried out when the land is not dried, and the watering quantity is preferably 70% of the wet land.
Comparative example 1
Selecting the leaves of the cymbidium faberi with roots of 6-10 cm, soaking the tissue culture seedlings with complete root systems and 2-6 developed leaves in the culture medium containing 10mg/ml IBA and 50mg/ml ZnSO 4 In the liquid culture of (1 d), only the root of the seedling is soaked by using a buoy, water mist is sprayed out by using a spray can to ensure that the seedling leaves are wet, the illumination intensity during treatment is 3000lx, the illumination time is 10 hours, and the transplanting and hardening seedlings are required to be placed at a hardening temperature of 25-28 ℃; after liquid culture, transplanting tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium to 80-85%, preventing the medium from being excessively wet, and not being suitable for watering when water exists, when the medium is not thoroughly dried, hardening seedlings by using an aqueous solution containing 10% ammonium bicarbonate, 5% superphosphate and 10% potassium chloride until the humidity is 70-80%, wherein the illumination intensity is 3000lx during hardening seedlings culture, and the light period of hardening seedlings culture is 10 hours in illumination duration; after seedling hardening and culturing for 30d, field planting, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
Comparative example 2
Selecting 6-10 cm of cymbidium leaves with roots, soaking tissue culture seedlings with complete root systems and 2-6 developed leaves in pure water-containing liquid culture for 1d, using a float to ensure that only the root parts of the seedlings are soaked, using a spray can to spray water mist to ensure that the seedling leaves are wet, and setting the illumination intensity at 3000lx and the illumination time at 10h, wherein the transplanting and hardening seedlings are required to be placed at hardening temperature of 25-28 ℃; after liquid culture, transplanting the tissue culture seedling into culture medium containing 0.5-0.8 cm sand stone, maintaining the humidity of the medium at 80-85%, preventing the medium from being excessively wet, and when the medium is not completely dried, using water containing 1% ammonium bicarbonate, 0.5% superphosphate and 1% potassium chloridePouring the mixture to the water solution with the humidity of 70-80% as a standard for hardening off, wherein the illumination intensity during hardening off cultivation is 3000lx, and the illumination period during hardening off cultivation is 10 hours; after seedling hardening and culturing for 30d, field planting, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
Comparative example 3
Selecting 6-10 cm of cymbidium leaves with roots, soaking tissue culture seedlings with complete root systems and 2-6 developed leaves in liquid culture containing 10mg/ml NAA and 50mg/ml LaCl for 1d, using a float to ensure that only the roots of the seedlings are soaked, using a spray can to spray water mist to ensure that the leaves of the seedlings are wet, ensuring that the illumination intensity is 3000lx during treatment, ensuring that the illumination time is 10h, and setting the transplanting and hardening seedlings at hardening temperature of 25-28 ℃; after liquid culture, transplanting tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium to 80-85%, preventing the medium from being excessively wet, and not being suitable for watering when water exists, when the medium is not thoroughly dried, watering to 70-80% humidity as a standard by using an aqueous solution containing 1% ammonium bicarbonate, 0.5% superphosphate and 1% potassium chloride, wherein the illumination intensity during seedling culture is 3000lx, and the light period during seedling culture is 10 hours of illumination duration; after seedling hardening and culturing for 30d, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
Comparative example 4
Selecting 6-10 cm of leaf and root of cymbidium, soaking the tissue culture seedling with complete root system and 2-6 developed leaves in liquid culture containing 10mg/ml NAA and 50mg/ml LaCl for 1d, using a float to ensure that only the root of the seedling is soaked, using a spray can to spray water mist to ensure that the leaf of the seedling is wet, the illumination intensity during treatment is 3000lx, the illumination time is 10h, and the transplanting and hardening seedling needs to be subjected to hardening at 25-28 DEG CSeedling temperature; after liquid culture, transplanting tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium at 80-85%, preventing the medium from being excessively wet, and when the medium is not thoroughly dried, watering with a pure water solution until the humidity is 70-80%, wherein the illumination intensity is 3000lx during seedling culture, and the light period of seedling culture is 10 hours; after seedling hardening and culturing for 30d, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
Comparative example 5
Selecting 6-10 cm of cymbidium leaves with roots, soaking tissue culture seedlings with complete root systems and 2-6 developing leaves in liquid culture containing 10mg/ml IBA for 1d, using a buoy to ensure that only the root parts of the seedlings are soaked, using a spray can to spray water mist to ensure that the seedling leaves are wet, enabling the illumination intensity to be 3000lx during treatment and enabling the illumination time to be 10h, and enabling transplanting and hardening seedlings to be placed at hardening temperature of 25-28 ℃; after liquid culture, transplanting tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium to 80-85%, preventing the medium from being excessively wet, and not being suitable for watering when water exists, when the medium is not thoroughly dried, watering to 70-80% humidity as a standard by using an aqueous solution containing 1% ammonium bicarbonate, 0.5% superphosphate and 1% potassium chloride, wherein the illumination intensity during seedling culture is 3000lx, and the light period during seedling culture is 10 hours of illumination duration; after seedling hardening and culturing for 30d, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
Comparative example 6
Selecting 6-10 cm of cymbidium leaves with roots, soaking 2-6 tissue culture seedlings with complete root systems and leaves in pure water-containing liquid culture for 1d, and ensuring by using a buoy during the periodOnly soaking the root of the seedling, spraying water mist by a spray can to ensure that the seedling leaves are moist, wherein the illumination intensity is 3000lx when in treatment, the illumination time is 10 hours, and the transplanting and hardening seedlings are required to be placed at hardening seedling temperature of 25-28 ℃; after liquid culture, transplanting tissue culture seedlings into a culture medium containing 0.5-0.8 cm sand stone, keeping the humidity of the medium at 80-85%, preventing the medium from being excessively wet, and when the medium is not thoroughly dried, watering with an aqueous solution until the humidity of 70-80% is used as a standard for seedling hardening, wherein the illumination intensity during seedling hardening culture is 3000lx, and the light period of seedling hardening culture is 10 hours; after seedling hardening and culturing for 30d, applying base fertilizer before field planting, wherein the base fertilizer is mainly organic fertilizer, and each 666.7m 2 Applying 800kg of commercial organic fertilizer Norsterra, 50kg of compound fertilizer (15-15-15), transplanting temperature of 28-35 ℃, illumination intensity of 5000lx, photoperiod of 10 hours of illumination duration, soil not suitable for being excessively wet, watering when the soil is not dry, and watering water amount suitable for the soil to be thoroughly wet.
After 60d of field planting, the content of each characteristic aroma substance in leaves of the cymbidium She Xinxian was measured, and the transplanting survival rate and aroma content of the tissue culture seedlings in example 1 and comparative examples 1 to 6 were counted, and the results are shown in table 1. The test method is as follows: (1) sample pretreatment: picking different processed herba Eupatorii leaves, wiping leaf surface impurities with gauze soaked in absolute ethyl alcohol, respectively weighing 4g of sheared herba Eupatorii leaves in a 50mL centrifuge tube, adding 12mL of absolute ethyl alcohol, immersing a sample with a glass rod, performing ultrasonic extraction at 400W, 40KHz and 50 ℃ for 60min, drying the extract liquid anhydrous sodium sulfate, filtering by a 0.22 μm organic phase needle filter (nylon), and preserving in a sample bottle at 4 ℃ in a refrigerator to be detected. (2) Meteorological chromatography-Mass Spectrometry (GC-MS) determination: meteorological chromatographic conditions: DB-WAX (30 m x 0.25mm x 0.25 μm) elastic quartz capillary column, carrier gas is high purity helium (99.999%), carrier gas flow rate is 1mL/min, sample is not split, sample inlet temperature is 250 ℃, interface temperature is 250 ℃. The initial temperature of the chromatographic column is 50 ℃, the chromatographic column is kept for 2min, the temperature is increased to 100 ℃ at 5 ℃/min, and then the chromatographic column is increased to 250 ℃ at 6 ℃/min, and the chromatographic column is kept for 5min; mass spectrometry conditions: the ion source is EI; a voltage of 70eV; the ion source temperature is 230 ℃; the temperature of the quadrupole rods is 150 ℃, the temperature of the sample inlet is 250 ℃, and the scanning mass range is 30-450 amu.
TABLE 1 comparison of transplanting survival rates and fragrance contents of tissue culture seedlings by different culture methods
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As can be seen from Table 1, the technical method for improving the transplanting survival rate and the fragrance of the cymbidium She Zupei seedlings has the transplanting survival rate of more than 98 percent. After the flavoring treatment provided by the invention is adopted for 2 months of cultivation, the content of 2-acetyl-1-pyrroline reaches 72 mug/g; the content of phytol reaches 111 mug/g; the squalene content reaches 302 mug/g, and compared with other control methods, the content of the 2-acetyl-1-pyrroline is improved by more than 20%, so that the flavoring effect is achieved, and the method is an optimal cultivation method.

Claims (8)

1. A method for improving the transplanting survival rate and fragrance of the cymbidium She Zupei seedlings, which is characterized by comprising the following steps:
s1) soaking the root of the root-bearing cymbidium She Zupei seedling in zinc sulfate solution for treatment; the concentration of the zinc sulfate solution is 30-80 mg/mL; the treatment time is 20-30 hours;
s2) transplanting the processed seedling She Zupei of the cymbidium into a culture medium, and performing seedling hardening culture by keeping the culture medium at 70% -85% humidity; the seedling hardening nutrient solution is used for keeping the humidity of the culture medium at 70% -85% during seedling hardening culture; the seedling hardening nutrient solution comprises 5-10 mg/L ammonium bicarbonate, 5-8 mg/L calcium superphosphate, 10-15 mg/L potassium chloride and water;
s3) planting the seedling-hardening cultured cymbidium sinense She Zupei seedlings.
2. The method of claim 1, wherein the root bearing cymbidium She Zupei seedlings are 6-10 cm high with a complete root system and 2-6 expanded leaves.
3. The method according to claim 1, wherein the blades are kept wet during the treatment in step S1); the temperature during treatment is 25-28 ℃; the illumination intensity during treatment is 2000-4000 lx; the illumination time during treatment is 6-12 h.
4. The method according to claim 1, wherein the cultivation substrate in the step S2) is 0.5-0.8 cm of sand.
5. The method according to claim 1, wherein the seedling hardening cultivation time in the step S2) is 20-40 days; the illumination intensity of seedling hardening culture is 2000-4000 lx; the light period of seedling hardening culture is 8-12 hours of illumination time.
6. The method according to claim 1, wherein the base fertilizer is applied before the planting in step S3); the base fertilizer comprises an organic fertilizer and a nitrogen-phosphorus-potassium compound fertilizer; the mass ratio of the organic fertilizer to the nitrogen-phosphorus-potassium compound fertilizer is (500-1000): (30-50).
7. The method according to claim 6, wherein the organic fertilizer is used in an amount of 500-1000 kg/666.7 m 2 。
8. The method according to claim 1, wherein the temperature of the field planting is 28 ℃ to 35 ℃; the illumination intensity during field planting is 3000-8000 lx; the light period of field planting is 8-12 hours of illumination time; the watering amount in field planting can wet the soil by 60% -80%.
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