CN114847161A - Method for improving transplanting survival rate and fragrance of leaf tissue culture seedlings of cymbidium sinense - Google Patents

Abstract

The present invention provides a method for improving the transplanted survival rate and fragrance of pandan leaf tissue culture seedlings, comprising: S1) soaking the roots of the rooted pandan leaf tissue culture seedlings in zinc sulfate solution for treatment; S2) treating The treated pandan leaf tissue culture seedlings are transplanted into a cultivation medium and the cultivation medium maintains a humidity of 70% to 85% for seedling cultivation; S3) Colonize the pandan blue leaf tissue culture seedlings after the tempering and cultivation. Compared with the prior art, the method provided by the invention can improve the transplanting survival rate of pandan leaf tissue culture seedlings, shorten the time of growing seedlings inside and outside the bottle of the tissue culture seedlings, and can also increase the content of the main aroma components of the pandan leaf tissue culture seedlings. In particular, the content of 2-acetyl-1-pyrroline effectively improves the production efficiency and seedling quality of pandan leaves.

Description

A method for improving the transplanted survival rate and fragrance of pandan leaf tissue culture seedlings

technical field

The invention belongs to the technical field of plant breeding and cultivation, and in particular relates to a method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings.

Background technique

Pandan leaf, scientific name Pandanus amaryllifolius, English translation trade name (Pandan leaf), domestic translation is Banlanxiang, also known as pandan leaf, pandan leaf, Banlan leaf, for the Pandanaceae (Pandanaceae) Pandanus is a perennial evergreen horticultural crop. There are about 800 species of plants in 3 genera of Pandanaceae. The native ecological environment of this genus is very different, and its economic uses are different. It can be used in medical and cooking fields. There are many species in the genus that can treat toothache and rheumatism. Development and utilization prospects are broad. Among them, pandan leaves is a plant with a unique "zongxiang" fragrance in this genus. In addition, the leaves of pandanlan are rich in active ingredients such as squalene, linoleic acid, wormwood and sterol, which can enhance cell vitality, speed up metabolism, improve human immunity, and inhibit the growth of cancer cells. vanilla". In addition to its good taste, pandan leaves have the effects of relieving summer heat, cooling off fire, calming the nerves, calming the nerves, relaxing the muscles and activating collaterals because of the strong anti-oxidant ingredients in its juice, which has a very high economic development value.

my country's pandan leaf is an imported crop from abroad. It was first introduced to the Xinglong area for planting, and then gradually developed to other areas of Hainan, Yunnan and other regions. Now, Guangdong, Taiwan and other provinces are also partially planted with pandan leaves. Many countries in Southeast Asia use its leaf juice as an added spice to improve the quality of cakes and beverages. It is one of the commonly used food spices in tropical regions. With pandan leaves as the main material, pandan cakes, pandan essence, pandan sauce, pandan sago, pandan syrup and other products have become famous special foods in tropical regions and are deeply loved by consumers. As a well-loved spice product and an important tropical agricultural product, there has been a problem of strong demand and insufficient supply for a long time, and with the continuous improvement of the quality of life, the demand for pandan leaves in my country will continue to increase. As a typical tropical spice crop, pandan leaf is of great significance for developing local characteristic economy and improving farmers' income and living standards in remote areas, and has broad prospects for development.

Due to the lack of flowers and fruits, the traditional planting of pandan leaves is mainly based on sub-seed breeding. This method has a growth degree of at least two years for the mother plant, and the seedlings that need to be tillered are limited, which cannot meet the requirements of the industry for high-throughput seedling breeding. develop. In addition, after the panda leaf tissue culture seedlings start to be propagated in large quantities, there will also be differences in growth during transplanting, and the transplanting survival rate will be low. And other issues.

SUMMARY OF THE INVENTION

In view of this, the technical problem to be solved by the present invention is to provide a method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings, which can significantly improve the transplanting survival rate and tissue culture of pandan leaf tissue culture seedlings. The fragrance of the seedlings, especially the content of 2-acetyl-1-pyrroline was significantly improved.

The invention provides a method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings, comprising:

S1) the root of the pandan blue leaf tissue culture seedling with roots is immersed in zinc sulfate solution and processed;

S2) transplanting the treated pandan leaf tissue culture seedling into a cultivation substrate and the cultivation substrate maintains a humidity of 70% to 85% for seedling cultivation;

S3) Colonize the tissue culture seedlings of pandan leaves after the hardening and cultivation.

Preferably, the tissue culture seedlings with rooted pandan leaves are 6-10 cm in height, have a complete root system and 2-6 expanded leaves.

Preferably, the concentration of the zinc sulfate solution is 30-80 mg/mL.

Preferably, the treatment time in the step S1) is 20-30h; the leaves are kept moist during the treatment; the temperature during the treatment is 25°C-28°C; the illumination intensity during the treatment is 2000-4000lx; the illumination time during the treatment is 6～12h.

Preferably, in the step S2), the cultivating medium is 0.5-0.8 cm of sand and gravel; the nutrient solution for cultivating seedlings is used to maintain a humidity of 70%-85% in the cultivating substrate; the nutrient solution for cultivating seedlings includes 5 mg/L ~10mg/L ammonium bicarbonate, 5mg/L~8mg/L superphosphate, 10mg/L~15mg/L potassium chloride and water.

Preferably, in the step S2), the time of seedling cultivation is 20-40 days; the light intensity of seedling cultivation is 2000-4000 lx; the photoperiod of seedling cultivation is 8-12 hours of light duration.

Preferably, in the step S3), base fertilizer is applied before planting; the base fertilizer includes organic fertilizer and NPK compound fertilizer; the mass ratio of the organic fertilizer to NPK compound fertilizer is (500～1000): (30～ 50).

Preferably, the usage amount of the organic fertilizer is 500-1000kg/666.7cm ² .

Preferably, the temperature of the colonization is 28°C to 35°C; the light intensity during the colonization is 3000 to 8000lx; the photoperiod of the colonization is 8 to 12 hours; %.

The invention provides a method for improving the transplanted survival rate and fragrance of pandan leaf tissue culture seedlings, comprising: S1) soaking the roots of the rooted pandan leaf tissue culture seedlings in zinc sulfate solution for treatment; S2) treating The treated pandan leaf tissue culture seedlings are transplanted into a cultivation substrate and the cultivation substrate maintains a humidity of 70% to 85% for seedling cultivation; S3) colonizing the patched pandan leaf tissue culture seedlings. Compared with the prior art, the method provided by the invention can improve the transplanting survival rate of pandan leaf tissue culture seedlings, shorten the time of growing seedlings inside the bottle of tissue culture seedlings and outside the bottle, and can also increase the content of main aroma components of the tissue culture seedlings of pandan leaves. In particular, the content of 2-acetyl-1-pyrroline can effectively improve the production efficiency of pandan leaves and the quality of seedlings.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

The invention provides a method for improving the transplanted survival rate and fragrance of pandan leaf tissue culture seedlings, comprising: S1) soaking the roots of the rooted pandan leaf tissue culture seedlings in zinc sulfate solution for treatment; S2) treating The treated pandan leaf tissue culture seedlings are transplanted into a cultivation substrate and the cultivation substrate maintains a humidity of 70% to 85% for seedling cultivation; S3) colonizing the patched pandan leaf tissue culture seedlings.

Wherein, in the present invention, there is no special restriction on the sources of all raw materials, and they can be commercially available.

In the present invention, the height of the rooted pandan leaf tissue culture seedling is preferably 6-10 cm; the rooted pandan blue leaf tissue culture seedling preferably has a complete root system and 2 to 6 developed leaves.

The roots of the rooted pandan leaf tissue culture seedlings are soaked in zinc sulfate solution for treatment; in the present invention, preferably after the root medium of the rooted pandan leaf tissue culture seedlings is washed, drain the water, and then The roots are immersed in a zinc sulfate solution for treatment; the concentration of the zinc sulfate solution is preferably 30-80 mg/mL, more preferably 40-70 mg/mL, more preferably 40-60 mg/mL, and more preferably 45-55 mg /mL, most preferably 50mg/mL; the treatment time is preferably 20-30h, more preferably 24-26h; the illumination intensity during the treatment is preferably 2000-4000lx; the illumination time during the treatment is preferably 6-12h During the treatment, the leaves are preferably kept moist, and it is more preferred to use a watering can to spray water mist to keep the leaves of the tissue culture seedlings moist; the temperature during the treatment is preferably 25°C to 28°C.

The treated pandan leaf tissue culture seedlings are transplanted into a cultivation substrate and the cultivation substrate maintains 70% to 85% humidity to carry out seedling cultivation; the cultivation substrate can be a cultivation substrate well known to those skilled in the art, and there is no special In the present invention, it is preferably 0.5-0.8cm sandstone; the humidity of the cultivation substrate during transplanting is preferably 80%-85%; in order to prevent the substrate from being too wet during seedling cultivation, it is not suitable to water when there is still moisture. Preferably, when the substrate is not completely dry, the nutrient solution for cultivating seedlings keeps the cultivation substrate at 70% to 85% humidity; in terms of mass fraction, the nutrient solution for cultivating seedlings preferably includes 5 mg/L to 10 mg/L ammonium bicarbonate, 5 mg/L ～8mg/L calcium superphosphate, 10mg/L～15mg/L potassium chloride and water, more preferably 8mg/L～10mg/L ammonium bicarbonate, 5mg/L～6mg/L calcium superphosphate, 10mg/L～ 12mg/L potassium chloride and water, preferably again include 10mg/L ammonium bicarbonate, 5mg/L superphosphate, 10mg/L potassium chloride and water; Preferably use the seedling nutrient solution to keep the cultivating substrate at 70°C during seedling cultivation. %～80% humidity; the time of the seedling cultivation is preferably 20 to 40 days, more preferably 25 to 35 days, and more preferably 30 days; the temperature of the seedling cultivation is preferably 25°C to 28°C; The light intensity during seedling cultivation is preferably 2000-4000 lx; the photoperiod of seedling cultivation is preferably 8-12 hours of light duration.

Finally, the tissue culture seedlings of pandan leaves after being hardened and cultivated are planted; before planting, base fertilizer is preferably applied; the base fertilizer is preferably based on organic fertilizer, and more preferably includes organic fertilizer and nitrogen, phosphorus and potassium compound fertilizer; the organic fertilizer is preferably High-quality organic fertilizer or commercial organic fertilizer; the NPK compound fertilizer is preferably compound fertilizer (15-15-15); the preferred mass ratio of the organic fertilizer to NPK compound fertilizer is (500-1000): (30 ～50); the usage amount of the organic fertilizer is 500～1000kg/666.7cm ² ; the temperature of the planting is 28 ℃～35 ℃; the light intensity during the planting is 3000～8000lx; the photoperiod of the planting is the light duration 8 ~12h; the land should not be too wet during planting, water when the land is still dry, and the amount of watering during planting will make the soil wet by 60% to 80%, more preferably 70%.

The method provided by the invention can improve the transplanting survival rate of pandan leaf tissue culture seedlings, shorten the time of growing the tissue culture seedlings inside the bottle and outside the bottle, and can also increase the content of the main aroma components, especially 2-acetyl-1, of the pandan leaf tissue culture seedlings -The content of pyrroline, thereby effectively improving the production efficiency of pandan leaves and the quality of seedlings.

In order to further illustrate the present invention, a method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings provided by the present invention will be described in detail below with reference to the examples.

The reagents used in the following examples are all commercially available.

Example 1

Select the tissue culture seedlings with leaves with roots of 6-10cm, complete root system and 2-6 developed leaves, and soak them in liquid culture containing 50mg/ml ZnSO ₄ for 1 day. The water mist ensures that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx and the light time is 10h. The transplanting and hardening should be placed at a temperature of 25-28 °C. After liquid culture, the tissue culture seedlings need to be transplanted to contain In the cultivation medium of 0.5-0.8cm sand and gravel, keep the humidity of the substrate at 80%-85% to prevent the substrate from getting too wet. It is not advisable to water when there is still moisture. % calcium superphosphate + 10% potassium chloride aqueous solution, poured to 70% ~ 80% humidity as the standard for seedling hardening, the light intensity during seedling hardening culture is 3000lx, and the photoperiod of seedling hardening culture is 10h of light duration; After cultivating for 30 days, the plant should be planted. Before planting, base fertilizer should be applied. The base fertilizer is mainly organic fertilizer. Every 666.7m2 is applied with commercial organic fertilizer ^Norsterra 800kg, compound fertilizer (15-15-15) 50kg, transplanting temperature 28 ~ 35 ℃, light intensity 5000lx , the photoperiod is 10h of light, the land should not be too wet, water when the land will be dry, and the watering amount should be 70% of the soil wet.

Comparative Example 1

Select the tissue culture seedlings with leaves with roots of 6-10cm, complete roots and 2-6 developed leaves and soak them in liquid culture containing 10mg/ml IBA and 50mg/ml ZnSO ₄ for 1 d. During this period, use floatation to ensure that only the seeds are soaked. At the roots of the seedlings, use a watering can to spray water mist to ensure that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx, and the light time is 10h. The transplanting and hardening should be placed at a temperature of 25-28 °C. The seedlings are transplanted into the cultivation medium containing 0.5-0.8cm sand and gravel, and the humidity of the substrate is kept at 80%-85% to prevent the substrate from getting too wet. The aqueous solution of % ammonium bicarbonate + 5% calcium superphosphate + 10% potassium chloride, poured to 70% ~ 80% humidity as the standard for seedling cultivation, the light intensity during seedling cultivation is 3000lx, and the photoperiod of seedling cultivation is The duration of light is 10h; the seedlings are planted after 30d of hardening and cultivation, and base fertilizer should be applied before planting. The base fertilizer is mainly organic fertilizer. Every 666.7m2 is applied with 800kg of commercial organic fertilizer Norsterra and 50kg of ^compound fertilizer (15-15-15), and the transplanting temperature is 28~ 35°C, light intensity 5000lx, photoperiod is 10h, the land should not be too wet, water when the land is still dry, and the watering amount should be 70% of the soil wet.

Comparative Example 2

Select the tissue culture seedlings with roots of 6-10 cm, complete root system and 2-6 developed leaves of pandanlan leaves and soak them in liquid culture containing pure water for 1 day. During this period, use floatation to ensure that only the roots of seedlings are soaked, and spray water mist with a watering can. Ensure that the leaves of the seedlings are moist, the light intensity during treatment is 3000lx, the light time is 10h, and the transplanting and hardening should be placed at a temperature of 25-28 °C; In the cultivation substrate of cm sandstone, keep the humidity of the substrate at 80% to 85% to prevent the substrate from getting too wet. It is not advisable to water when there is still moisture. When the substrate is not dry, use 1% ammonium bicarbonate + 0.5% superphosphoric acid. The aqueous solution of calcium + 1% potassium chloride is poured to 70% to 80% humidity as the standard for seedling cultivation. The light intensity during seedling cultivation is 3000lx, and the photoperiod of seedling cultivation is 10h of light duration; after cultivation for 30 days Planting, base fertilizer should be applied before planting, the base fertilizer is mainly organic fertilizer, commercial organic fertilizer ^Norsterra 800kg, compound fertilizer (15-15-15) 50kg per 666.7m2, transplanting temperature 28 ~ 35 ℃, light intensity 5000lx, photoperiod The duration of light is 10h, and the land should not be too wet. Water when the land is still dry, and the watering amount should be 70% of the soil wet.

Comparative Example 3

Select the tissue culture seedlings with a root of 6-10 cm, complete root system and 2-6 developing leaves of pandanlan leaves and soak them in a liquid culture containing 10 mg/ml NAA and 50 mg/ml LaCl for 1 day, during which time use floatation to ensure that only the seedlings are soaked At the roots, spray water mist with a watering can to ensure that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx, and the light time is 10h. The transplanting and hardening should be placed at a temperature of 25-28 °C; The seedlings are transplanted into the cultivation medium containing 0.5-0.8cm sand and gravel, and the humidity of the substrate should be kept at 80%-85% to prevent the substrate from getting too wet. An aqueous solution of ammonium bicarbonate + 0.5% superphosphate + 1% potassium chloride, poured to 70% to 80% humidity as the standard for seedling hardening, the light intensity during seedling hardening and cultivation is 3000lx, and the photoperiod of seedling cultivation is light. The duration is 10h; after cultivating for 30d, base fertilizer should be applied before planting. The base fertilizer is mainly organic fertilizer. Every 666.7m2 should be applied with commercial organic fertilizer ^Norsterra 800kg, compound fertilizer (15-15-15) 50kg, and the transplanting temperature is 28～35℃ , The light intensity is 5000lx, the light period is 10h, the land should not be too wet, water when the land will be dry, and the watering amount should be 70% wet.

Comparative Example 4

Select the tissue culture seedlings with a root of 6-10 cm, complete root system and 2-6 developing leaves of pandanlan leaves and soak them in a liquid culture containing 10 mg/ml NAA and 50 mg/ml LaCl for 1 day, during which time use floatation to ensure that only the seedlings are soaked At the roots, spray water mist with a watering can to ensure that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx, and the light time is 10h. The transplanting and hardening should be placed at a temperature of 25-28 °C; The seedlings are transplanted into a cultivation medium containing 0.5-0.8cm sand and gravel, and the humidity of the substrate should be kept at 80%-85% to prevent the substrate from being too wet. Pour to 70% ~ 80% humidity as the standard for seedling hardening. The light intensity during seedling hardening is 3000lx, and the photoperiod of hardening culture is 10h. Fertilizer is the main fertilizer, commercial organic fertilizer ^Norsterra 800kg, compound fertilizer (15-15-15) 50kg are applied per 666.7m2, the transplanting temperature is 28~35℃, the light intensity is 5000lx, and the photoperiod is 10h. The soil should be watered when it is still dry, and the watering amount should be 70% of the soil wet.

Comparative Example 5

Select the tissue culture seedlings with leaves with roots of 6-10cm, complete root system and 2-6 developed leaves and soak them in liquid culture containing 10mg/ml IBA for 1 day. The water mist ensures that the leaves of the seedlings are moist. The light intensity during treatment is 3000lx and the light time is 10h. The transplanting and hardening should be placed at a temperature of 25-28 °C. After liquid culture, the tissue culture seedlings need to be transplanted to contain In the cultivation substrate of 0.5-0.8cm sand and gravel, keep the humidity of the substrate at 80%-85% to prevent the substrate from getting too wet. It is not advisable to water when there is still moisture. The aqueous solution of % calcium superphosphate + 1% potassium chloride, poured to 70% ~ 80% humidity as the standard for seedling hardening, the light intensity during seedling hardening culture is 3000lx, and the photoperiod of seedling hardening culture is 10h of light duration; After cultivating for 30 days, base fertilizer should be applied before planting. The base fertilizer is mainly organic fertilizer. Every 666.7m2 is applied with commercial organic fertilizer ^Norsterra 800kg, compound fertilizer (15-15-15) 50kg, transplanting temperature 28 ~ 35 ℃, light intensity 5000lx, The photoperiod is 10 hours of light, and the soil should not be too wet. Water when the soil is still dry, and the amount of water should be 70% of the soil wet.

Comparative Example 6

Select the tissue culture seedlings with roots of 6-10 cm, complete root system and 2-6 developed leaves of pandanlan leaves and soak them in liquid culture containing pure water for 1 day. During this period, use floatation to ensure that only the roots of seedlings are soaked, and spray water mist with a watering can. Ensure that the leaves of the seedlings are moist, the light intensity during treatment is 3000lx, the light time is 10h, and the transplanting and hardening should be placed at a temperature of 25-28 °C; In the cultivation medium of cm sandstone, keep the humidity of the substrate at 80% to 85% to prevent the substrate from getting too wet. It is not advisable to water when there is still water. Standard seedling hardening, the light intensity during hardening and culturing is 3000lx, and the photoperiod for hardening and cultivating is 10h of light duration; after 30 days of hardening and cultivating, base fertilizer should be applied before planting, and the base fertilizer is mainly organic fertilizer, applied every ^666.7m Commercial organic fertilizer Norsterra 800kg, compound fertilizer (15-15-15) 50kg, transplanting temperature 28～35℃, light intensity 5000lx, photoperiod is 10h light duration, the land should not be too wet, water when the land is still dry , the watering amount should be 70% of the soil wet.

After 60 days of planting, the content of various characteristic aroma substances in the fresh leaves of pandan leaves was measured, and the transplanted survival rate and aroma content of tissue culture seedlings in Example 1 and Comparative Examples 1 to 6 were counted. The results are shown in Table 1. The test method is as follows: (1) Sample pretreatment: Pick the pandan leaves with different treatments, wipe off the impurities on the leaf surface with gauze soaked in absolute ethanol, and weigh 4g of the cut pandan leaves in a 50mL centrifuge tube, respectively. Add 12 mL of absolute ethanol and immerse the sample with a glass rod. Ultrasonic extraction was performed at 400W, 40KHz, and 50 °C for 60 min. After the extract was dried with anhydrous sodium sulfate, it was filtered through a 0.22 μm organic phase needle filter (nylon) and injected into the sample. in a bottle and stored in a refrigerator at 4°C for testing. (2) Determination by gas chromatography-mass spectrometry (GC-MS): gas chromatography conditions: DB-WAX (30m×0.25mm×0.25μm) elastic quartz capillary column, the carrier gas is high-purity helium (99.999%), The carrier gas flow was 1 mL/min, the sample was splitless, the temperature of the injection port was 250 °C, and the temperature of the interface was 250 °C. The initial temperature of the chromatographic column was 50 °C, maintained for 2 min, heated to 100 °C at 5 °C/min, and then heated to 250 °C at 6 °C/min, held for 5 min; mass spectrometry conditions: ion source source was EI; voltage 70eV; ion source temperature 230 ℃; the quadrupole temperature is 150℃, the inlet temperature is 250℃, and the scanning mass range is 30～450amu.

Table 1 Comparison of survival rate and aroma content of tissue culture seedlings transplanted with different culture methods

As can be seen from Table 1, by adopting a technical method for improving the transplanting survival rate and fragrance of pandan leaf tissue culture seedlings of the present invention, the transplanting survival rate reaches more than 98%. After culturing for 2 months with the aroma-enhancing treatment provided by the invention, the content of 2-acetyl-1-pyrroline reaches 72 μg/g; the content of phytol reaches 111 μg/g; the content of squalene reaches 302 μg/g, compared with In other control methods, the content of 2-acetyl-1-pyrroline was increased by more than 20%, which had an aroma-enhancing effect and was the optimal cultivation method.

Claims (9)

1. a method for improving the transplanting survival rate of pandan leaf tissue culture seedlings and fragrance, is characterized in that, comprises: S1) the root of the pandan blue leaf tissue culture seedling with roots is immersed in zinc sulfate solution and processed; S2) transplanting the treated pandan leaf tissue culture seedling into a cultivation substrate and the cultivation substrate maintains a humidity of 70% to 85% for seedling cultivation; S3) Colonize the tissue culture seedlings of pandan leaves after the hardening and cultivation. 2 . The method according to claim 1 , wherein the tissue culture seedlings of pandan blue leaves with roots are 6-10 cm high, have a complete root system and 2-6 expanded leaves. 3 . 3. The method according to claim 1, wherein the concentration of the zinc sulfate solution is 30-80 mg/mL. 4. The method according to claim 1, wherein the treatment time in the step S1) is 20-30h; the leaves are kept moist during the treatment; the temperature during the treatment is 25°C-28°C; the illumination during the treatment The intensity is 2000～4000lx; the illumination time during treatment is 6～12h. 5. method according to claim 1, is characterized in that, in described step S2), cultivation substrate is the sandstone of 0.5～0.8cm; Use hardening nutrient solution to make cultivation substrate keep 70%～85% during seedling cultivation Humidity; the seedling nutrient solution includes 5mg/L-10mg/L ammonium bicarbonate, 5mg/L-8mg/L superphosphate, 10mg/L-15mg/L potassium chloride and water. 6. method according to claim 1, is characterized in that, in described step S2), the time of cultivating seedlings is 20～40 days; The light intensity of cultivating seedlings is 2000～4000lx; The photoperiod of cultivating seedlings is The duration of light is 8 to 12 hours. 7. method according to claim 1, is characterized in that, in described step S3), apply basal fertilizer before planting; Described basal fertilizer comprises organic fertilizer and NPK compound fertilizer; Described organic fertilizer and NPK compound fertilizer The mass ratio is (500-1000):(30-50). 8 . The method according to claim 7 , wherein the usage amount of the organic fertilizer is 500-1000 kg/666.7 cm ² . 9 . The method according to claim 1 , wherein the temperature of the colonization is 28° C. to 35° C.; the light intensity during the colonization is 3000 to 8000lx; the photoperiod of the colonization is 8 to 12 hours of light duration; The amount of watering makes the soil soaked by 60% to 80%.