CN105820355A - 京尼平交联鱼源胶原复合材料及其制备方法 - Google Patents
京尼平交联鱼源胶原复合材料及其制备方法 Download PDFInfo
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- CN105820355A CN105820355A CN201610206505.2A CN201610206505A CN105820355A CN 105820355 A CN105820355 A CN 105820355A CN 201610206505 A CN201610206505 A CN 201610206505A CN 105820355 A CN105820355 A CN 105820355A
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- fish
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 104
- 102000008186 Collagen Human genes 0.000 title claims abstract description 102
- 108010035532 Collagen Proteins 0.000 title claims abstract description 102
- 229920001436 collagen Polymers 0.000 title claims abstract description 80
- AZKVWQKMDGGDSV-BCMRRPTOSA-N Genipin Chemical compound COC(=O)C1=CO[C@@H](O)[C@@H]2C(CO)=CC[C@H]12 AZKVWQKMDGGDSV-BCMRRPTOSA-N 0.000 title claims abstract description 58
- AZKVWQKMDGGDSV-UHFFFAOYSA-N genipin Natural products COC(=O)C1=COC(O)C2C(CO)=CCC12 AZKVWQKMDGGDSV-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 239000002131 composite material Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 37
- 239000002904 solvent Substances 0.000 claims abstract description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 51
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 45
- 239000000243 solution Substances 0.000 claims description 36
- 238000004132 cross linking Methods 0.000 claims description 29
- 239000012153 distilled water Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000007864 aqueous solution Substances 0.000 claims description 16
- 238000000502 dialysis Methods 0.000 claims description 16
- 102000057297 Pepsin A Human genes 0.000 claims description 14
- 108090000284 Pepsin A Proteins 0.000 claims description 14
- 239000003431 cross linking reagent Substances 0.000 claims description 14
- 229940111202 pepsin Drugs 0.000 claims description 14
- 241000276707 Tilapia Species 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 241000238366 Cephalopoda Species 0.000 claims description 10
- 239000008363 phosphate buffer Substances 0.000 claims description 10
- 238000013019 agitation Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 7
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000005238 degreasing Methods 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 6
- 239000011780 sodium chloride Substances 0.000 claims 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000007541 cellular toxicity Effects 0.000 abstract 1
- 238000005185 salting out Methods 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 6
- 239000012620 biological material Substances 0.000 description 4
- 230000003013 cytotoxicity Effects 0.000 description 4
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- 238000011010 flushing procedure Methods 0.000 description 4
- 238000007654 immersion Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- IBFYXTRXDNAPMM-BVTMAQQCSA-N Geniposide Chemical compound O([C@@H]1OC=C([C@@H]2[C@H]1C(=CC2)CO)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O IBFYXTRXDNAPMM-BVTMAQQCSA-N 0.000 description 3
- IBFYXTRXDNAPMM-FZEIBHLUSA-N Geniposide Natural products COC(=O)C1=CO[C@@H](O[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O)[C@H]2[C@@H]1CC=C2CO IBFYXTRXDNAPMM-FZEIBHLUSA-N 0.000 description 3
- VGLLGNISLBPZNL-RBUKDIBWSA-N arborescoside Natural products O=C(OC)C=1[C@@H]2C([C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)OC=1)=C(CO)CC2 VGLLGNISLBPZNL-RBUKDIBWSA-N 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
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- 125000000524 functional group Chemical group 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 229920000954 Polyglycolide Polymers 0.000 description 1
- 241000657665 Rhopilema esculentum Species 0.000 description 1
- 208000018756 Variant Creutzfeldt-Jakob disease Diseases 0.000 description 1
- 208000005881 bovine spongiform encephalopathy Diseases 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000515 collagen sponge Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 239000004633 polyglycolic acid Substances 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
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- 230000008961 swelling Effects 0.000 description 1
- 239000002407 tissue scaffold Substances 0.000 description 1
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- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
Description
Claims (10)
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CN201610206505.2A CN105820355B (zh) | 2016-04-05 | 2016-04-05 | 京尼平交联鱼源胶原复合材料及其制备方法 |
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CN201610206505.2A CN105820355B (zh) | 2016-04-05 | 2016-04-05 | 京尼平交联鱼源胶原复合材料及其制备方法 |
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CN105820355B CN105820355B (zh) | 2019-12-27 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107936579A (zh) * | 2017-12-19 | 2018-04-20 | 中国科学院烟台海岸带研究所 | 一种双网络水凝胶的制备方法 |
CN108329634A (zh) * | 2017-01-20 | 2018-07-27 | 中国科学院烟台海岸带研究所 | 胶原蛋白/碳纳米管/聚丙烯酰胺复合水凝胶及其制备方法 |
CN109354886A (zh) * | 2018-11-07 | 2019-02-19 | 中国科学院烟台海岸带研究所 | 复合水凝胶及其制备方法 |
CN113372436A (zh) * | 2021-07-08 | 2021-09-10 | 中国海洋大学 | 一种鱼皮源外层致密内层疏松的医用胶原膜及其制备方法 |
CN116575241A (zh) * | 2023-03-24 | 2023-08-11 | 南通通州江华纺织有限公司 | 一种基于纳米纤维素纤维的面膜基布及其制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103007357A (zh) * | 2012-12-25 | 2013-04-03 | 福建省博特生物科技有限公司 | 一种碳纳米管/胶原基复合材料的应用 |
CN103690996A (zh) * | 2013-12-26 | 2014-04-02 | 中国水产科学研究院长江水产研究所 | 一种基于鱼皮胶原蛋白的生物复合材料及制备方法 |
CN104031274A (zh) * | 2014-05-28 | 2014-09-10 | 中国科学院烟台海岸带研究所 | 一种水产鱼皮胶原蛋白水凝胶的制备方法 |
CN104027835A (zh) * | 2014-05-28 | 2014-09-10 | 中国科学院烟台海岸带研究所 | 一种水产鱼皮胶原蛋白纳吸棉及其制备方法 |
-
2016
- 2016-04-05 CN CN201610206505.2A patent/CN105820355B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103007357A (zh) * | 2012-12-25 | 2013-04-03 | 福建省博特生物科技有限公司 | 一种碳纳米管/胶原基复合材料的应用 |
CN103690996A (zh) * | 2013-12-26 | 2014-04-02 | 中国水产科学研究院长江水产研究所 | 一种基于鱼皮胶原蛋白的生物复合材料及制备方法 |
CN104031274A (zh) * | 2014-05-28 | 2014-09-10 | 中国科学院烟台海岸带研究所 | 一种水产鱼皮胶原蛋白水凝胶的制备方法 |
CN104027835A (zh) * | 2014-05-28 | 2014-09-10 | 中国科学院烟台海岸带研究所 | 一种水产鱼皮胶原蛋白纳吸棉及其制备方法 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108329634A (zh) * | 2017-01-20 | 2018-07-27 | 中国科学院烟台海岸带研究所 | 胶原蛋白/碳纳米管/聚丙烯酰胺复合水凝胶及其制备方法 |
CN107936579A (zh) * | 2017-12-19 | 2018-04-20 | 中国科学院烟台海岸带研究所 | 一种双网络水凝胶的制备方法 |
CN109354886A (zh) * | 2018-11-07 | 2019-02-19 | 中国科学院烟台海岸带研究所 | 复合水凝胶及其制备方法 |
CN113372436A (zh) * | 2021-07-08 | 2021-09-10 | 中国海洋大学 | 一种鱼皮源外层致密内层疏松的医用胶原膜及其制备方法 |
CN116575241A (zh) * | 2023-03-24 | 2023-08-11 | 南通通州江华纺织有限公司 | 一种基于纳米纤维素纤维的面膜基布及其制备方法 |
CN116575241B (zh) * | 2023-03-24 | 2023-10-31 | 南通通州江华纺织有限公司 | 一种基于纳米纤维素纤维的面膜基布及其制备方法 |
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Effective date of registration: 20201225 Address after: 246001 e-commerce Industrial Park, NO.48 Renmin Road, Yingjiang District, Anqing City, Anhui Province Patentee after: Wang Zhao Address before: 266000 Heilongjiang Middle Road, Licang District, Qingdao City, Shandong Province Patentee before: Li Jie |
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Effective date of registration: 20211209 Address after: 257000 room 320, Wanda harbor city, No. 25-1, Gangcheng Road, dongyinggang Economic Development Zone, Dongying City, Shandong Province Patentee after: Dongying Yuchen Technology Co.,Ltd. Address before: 246001 e-commerce Industrial Park, NO.48 Renmin Road, Yingjiang District, Anqing City, Anhui Province Patentee before: Wang Zhao |