CN105779488A - 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 - Google Patents
一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 Download PDFInfo
- Publication number
- CN105779488A CN105779488A CN201610171174.3A CN201610171174A CN105779488A CN 105779488 A CN105779488 A CN 105779488A CN 201610171174 A CN201610171174 A CN 201610171174A CN 105779488 A CN105779488 A CN 105779488A
- Authority
- CN
- China
- Prior art keywords
- sequence
- promoter
- exogenous gene
- expression
- mmp1
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 195
- 241000894006 Bacteria Species 0.000 title claims abstract description 149
- 230000001939 inductive effect Effects 0.000 title claims abstract description 40
- 230000014509 gene expression Effects 0.000 claims abstract description 185
- 230000001276 controlling effect Effects 0.000 claims abstract description 73
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 68
- 238000000034 method Methods 0.000 claims abstract description 62
- 102000009661 Repressor Proteins Human genes 0.000 claims abstract description 18
- 108010034634 Repressor Proteins Proteins 0.000 claims abstract description 18
- 230000001105 regulatory effect Effects 0.000 claims abstract description 5
- 125000003729 nucleotide group Chemical group 0.000 claims description 101
- 239000013598 vector Substances 0.000 claims description 66
- 235000018102 proteins Nutrition 0.000 claims description 58
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 36
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 36
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 30
- 108020004414 DNA Proteins 0.000 claims description 25
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims description 24
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims description 22
- 230000001580 bacterial effect Effects 0.000 claims description 17
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical group N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 16
- 230000008034 disappearance Effects 0.000 claims description 12
- 230000008676 import Effects 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 11
- 108091026890 Coding region Proteins 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 9
- 230000033228 biological regulation Effects 0.000 claims description 9
- 101150099105 alien gene Proteins 0.000 claims description 7
- 125000000539 amino acid group Chemical group 0.000 claims description 6
- 230000010354 integration Effects 0.000 claims description 5
- 108091008324 binding proteins Proteins 0.000 claims description 4
- 102000023732 binding proteins Human genes 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 229920002477 rna polymer Polymers 0.000 abstract 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 description 95
- 239000005090 green fluorescent protein Substances 0.000 description 73
- 230000035772 mutation Effects 0.000 description 71
- 239000012634 fragment Substances 0.000 description 49
- 239000013612 plasmid Substances 0.000 description 43
- 101001013150 Homo sapiens Interstitial collagenase Proteins 0.000 description 22
- 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 description 22
- 241000206596 Halomonas Species 0.000 description 19
- 239000001963 growth medium Substances 0.000 description 14
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 230000006801 homologous recombination Effects 0.000 description 10
- 238000002744 homologous recombination Methods 0.000 description 10
- 244000005700 microbiome Species 0.000 description 10
- 230000009466 transformation Effects 0.000 description 10
- 230000006698 induction Effects 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 241000589516 Pseudomonas Species 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 239000013642 negative control Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- 102000009027 Albumins Human genes 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
- 229940097572 chloromycetin Drugs 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 101150109249 lacI gene Proteins 0.000 description 4
- 230000009182 swimming Effects 0.000 description 4
- 108010077805 Bacterial Proteins Proteins 0.000 description 3
- 108010072454 CTGCAG-specific type II deoxyribonucleases Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091092724 Noncoding DNA Proteins 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000286904 Leptothecata Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 238000009655 industrial fermentation Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229920000070 poly-3-hydroxybutyrate Polymers 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 241000607528 Aeromonas hydrophila Species 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005782 double-strand break Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- -1 nucleoside monophosphate Chemical class 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/002—Vectors comprising a special translation-regulating system controllable or inducible
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610171174.3A CN105779488B (zh) | 2016-03-23 | 2016-03-23 | 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610171174.3A CN105779488B (zh) | 2016-03-23 | 2016-03-23 | 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105779488A true CN105779488A (zh) | 2016-07-20 |
CN105779488B CN105779488B (zh) | 2018-06-22 |
Family
ID=56391457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610171174.3A Active CN105779488B (zh) | 2016-03-23 | 2016-03-23 | 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105779488B (zh) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977890A (zh) * | 2017-06-05 | 2018-12-11 | 北京蓝晶微生物科技有限公司 | 一种启动子库以及利用其在细菌中构建不同强度表达系统的方法 |
CN110004182A (zh) * | 2019-04-02 | 2019-07-12 | 清华大学 | 一种微生物胞内大颗粒内含物的制备方法及其应用 |
JPWO2020174988A1 (zh) * | 2019-02-28 | 2020-09-03 | ||
WO2021049207A1 (ja) * | 2019-09-09 | 2021-03-18 | 株式会社カネカ | 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法 |
CN113621639A (zh) * | 2021-09-02 | 2021-11-09 | 天津大学 | 重组嗜盐单胞菌及构建方法与催化丙酮酸生产乙偶姻的应用 |
CN116083465A (zh) * | 2023-01-03 | 2023-05-09 | 态创生物科技(广州)有限公司 | 乳糖负传感质粒及负反馈乳糖生物传感器 |
CN116254268A (zh) * | 2023-05-11 | 2023-06-13 | 北京蓝晶微生物科技有限公司 | 一种启动子库及其在不同细菌中的应用 |
CN116288740A (zh) * | 2023-05-11 | 2023-06-23 | 北京蓝晶微生物科技有限公司 | 一种微生物启动子库及其应用 |
CN116814615A (zh) * | 2023-08-30 | 2023-09-29 | 清华大学 | 一种细胞形态纤长化的重组菌株及其构建方法和应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6569669B1 (en) * | 2000-10-12 | 2003-05-27 | New England Biolabs, Inc. | Host strain for low uninduced expression of foreign RNA polymerase genes |
CN1604960A (zh) * | 2001-12-12 | 2005-04-06 | 伊莱利利公司 | 表达系统 |
US20060068469A1 (en) * | 2004-08-17 | 2006-03-30 | Research Development Foundation | Bacterial vector systems |
CN102264905A (zh) * | 2008-10-30 | 2011-11-30 | 基因桥有限责任公司 | 用于多肽重组表达的基于t7双启动子的载体系统 |
-
2016
- 2016-03-23 CN CN201610171174.3A patent/CN105779488B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6569669B1 (en) * | 2000-10-12 | 2003-05-27 | New England Biolabs, Inc. | Host strain for low uninduced expression of foreign RNA polymerase genes |
CN1604960A (zh) * | 2001-12-12 | 2005-04-06 | 伊莱利利公司 | 表达系统 |
US20060068469A1 (en) * | 2004-08-17 | 2006-03-30 | Research Development Foundation | Bacterial vector systems |
CN102264905A (zh) * | 2008-10-30 | 2011-11-30 | 基因桥有限责任公司 | 用于多肽重组表达的基于t7双启动子的载体系统 |
Non-Patent Citations (4)
Title |
---|
SCHOLL,D.等: "RNA polymerase [Enterobacteria phage K1F]", 《GENBANK登陆号:YP_338094.1》 * |
WANG,D.等: "RNA polymerase [Vibriophage VP4]", 《GENBANK登陆号:YP_249577.1》 * |
ZHU,J等: "RNA polymerase [Morganella phage MmP1]", 《GENBANK登陆号:YP_003324580.1》 * |
陈长征,等: "利用重组噬菌体诱导表达的大肠杆菌表达系统", 《生物工程学报》 * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977890B (zh) * | 2017-06-05 | 2022-02-15 | 北京蓝晶微生物科技有限公司 | 一种启动子库以及利用其在细菌中构建不同强度表达系统的方法 |
CN108977890A (zh) * | 2017-06-05 | 2018-12-11 | 北京蓝晶微生物科技有限公司 | 一种启动子库以及利用其在细菌中构建不同强度表达系统的方法 |
JPWO2020174988A1 (zh) * | 2019-02-28 | 2020-09-03 | ||
WO2020174988A1 (ja) * | 2019-02-28 | 2020-09-03 | 株式会社カネカ | 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法 |
CN110004182A (zh) * | 2019-04-02 | 2019-07-12 | 清华大学 | 一种微生物胞内大颗粒内含物的制备方法及其应用 |
CN114616320A (zh) * | 2019-09-09 | 2022-06-10 | 株式会社钟化 | 转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法 |
WO2021049207A1 (ja) * | 2019-09-09 | 2021-03-18 | 株式会社カネカ | 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法 |
CN113621639A (zh) * | 2021-09-02 | 2021-11-09 | 天津大学 | 重组嗜盐单胞菌及构建方法与催化丙酮酸生产乙偶姻的应用 |
CN113621639B (zh) * | 2021-09-02 | 2022-06-07 | 天津大学 | 重组嗜盐单胞菌及构建方法与催化丙酮酸生产乙偶姻的应用 |
CN116083465A (zh) * | 2023-01-03 | 2023-05-09 | 态创生物科技(广州)有限公司 | 乳糖负传感质粒及负反馈乳糖生物传感器 |
CN116083465B (zh) * | 2023-01-03 | 2024-01-05 | 态创生物科技(广州)有限公司 | 乳糖负传感质粒及负反馈乳糖生物传感器 |
CN116254268A (zh) * | 2023-05-11 | 2023-06-13 | 北京蓝晶微生物科技有限公司 | 一种启动子库及其在不同细菌中的应用 |
CN116288740A (zh) * | 2023-05-11 | 2023-06-23 | 北京蓝晶微生物科技有限公司 | 一种微生物启动子库及其应用 |
CN116254268B (zh) * | 2023-05-11 | 2023-08-15 | 北京蓝晶微生物科技有限公司 | 一种启动子库及其在不同细菌中的应用 |
CN116288740B (zh) * | 2023-05-11 | 2023-08-29 | 北京蓝晶微生物科技有限公司 | 一种微生物启动子库及其应用 |
CN116814615A (zh) * | 2023-08-30 | 2023-09-29 | 清华大学 | 一种细胞形态纤长化的重组菌株及其构建方法和应用 |
CN116814615B (zh) * | 2023-08-30 | 2024-01-19 | 清华大学 | 一种细胞形态纤长化的重组菌株及其构建方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN105779488B (zh) | 2018-06-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105779488A (zh) | 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用 | |
Bu et al. | Rational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches | |
Mï¿ ½ller et al. | Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones | |
Zhang et al. | Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis | |
Herman et al. | The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation | |
CN108977890B (zh) | 一种启动子库以及利用其在细菌中构建不同强度表达系统的方法 | |
CN103710374B (zh) | 一种5‑氨基乙酰丙酸产生菌株及其制备方法与应用 | |
Xiao et al. | Developing a Cas9‐based tool to engineer native plasmids in Synechocystis sp. PCC 6803 | |
Zhang et al. | Improving phloroglucinol tolerance and production in Escherichia coli by GroESL overexpression | |
Matsudo et al. | CO2 from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source | |
JP2019141106A (ja) | スピルリナにおける標的化された突然変異誘発 | |
Gao et al. | Mutations in genes encoding antibiotic substances increase the synthesis of poly‐γ‐glutamic acid in Bacillus amyloliquefaciens LL3 | |
Yu et al. | Genome shuffling of Streptomyces roseosporus for improving daptomycin production | |
Seo et al. | Development of an oxygen-independent flavin mononucleotide-based fluorescent reporter system in Clostridium beijerinckii and its potential applications | |
Li et al. | Investigation of secondary metabolism in the industrial butanol hyper-producer Clostridium saccharoperbutylacetonicum N1-4 | |
CN104946552A (zh) | 安全高效生产申嗪霉素的基因工程菌株及其应用 | |
Wang et al. | Rational selection and engineering of exogenous principal sigma factor (σ HrdB) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus | |
CN105969712B (zh) | 一种共表达分子伴侣蛋白提高重组大肠杆菌1,2,4-丁三醇产量的方法 | |
CN103290032B (zh) | 硫藤黄链霉菌抗生素调控基因及提高链霉菌抗生素产量的方法 | |
CN104204206B (zh) | 一种用于生产丁醇的方法 | |
CN108611357B (zh) | 一种重组表达质粒、转化子及其应用 | |
CN111041020B (zh) | 异柠檬酸裂合酶突变体、突变基因及其在制备维生素b12中的应用 | |
Petersen et al. | Enzymatic characterization of a nonmotile, nonsolventogenic Clostridium acetobutylicum ATCC 824 mutant | |
CN105200035B (zh) | 高gc含量大片段dna的体外组装方法及应用 | |
CN102154191B (zh) | 产2s-甲基丙二酰辅酶a的假单胞工程菌 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20161213 Address after: 100084 Haidian District, Beijing,,, Tsinghua University, the 100084 letter box office Applicant after: Qinghua UNiversity Applicant after: Aquamarine (Beijing) biotechnology limited liability company Address before: 100084 Haidian District, Beijing,,, Tsinghua University, the 100084 letter box office Applicant before: Qinghua UNiversity |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181219 Address after: 100084 Tsinghua University, Beijing, Haidian District Co-patentee after: Shenzhen Blue Crystal Biotechnology Co., Ltd. Patentee after: Tsinghua University Address before: 100084 Beijing Haidian District Beijing 100084 box 82 box, Tsinghua University Patent Office Co-patentee before: Aquamarine (Beijing) biotechnology limited liability company Patentee before: Tsinghua University |