CN105683373A - 外源性基因表达增强剂 - Google Patents

外源性基因表达增强剂 Download PDF

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Publication number
CN105683373A
CN105683373A CN201480058676.4A CN201480058676A CN105683373A CN 105683373 A CN105683373 A CN 105683373A CN 201480058676 A CN201480058676 A CN 201480058676A CN 105683373 A CN105683373 A CN 105683373A
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Prior art keywords
sequence
silkworm
gene
expression
repetitive
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CN201480058676.4A
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English (en)
Chinese (zh)
Inventor
立松谦郎
立松谦一郎
濑筒秀树
内野惠郎
小岛桂
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National Institute of Agrobiological Sciences
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National Institute of Agrobiological Sciences
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Publication of CN105683373A publication Critical patent/CN105683373A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A01K2217/058—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function due to expression of inhibitory nucleic acid, e.g. siRNA, antisense
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
CN201480058676.4A 2013-10-25 2014-10-14 外源性基因表达增强剂 Pending CN105683373A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013-222138 2013-10-25
JP2013222138 2013-10-25
PCT/JP2014/077316 WO2015060159A1 (ja) 2013-10-25 2014-10-14 外因性遺伝子発現増強剤

Publications (1)

Publication Number Publication Date
CN105683373A true CN105683373A (zh) 2016-06-15

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Family Applications (1)

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CN201480058676.4A Pending CN105683373A (zh) 2013-10-25 2014-10-14 外源性基因表达增强剂

Country Status (3)

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JP (1) JP6326703B2 (ja)
CN (1) CN105683373A (ja)
WO (1) WO2015060159A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471714A (zh) * 2020-05-07 2020-07-31 西南大学 Minos转座子系统介导的真核生物转基因细胞系及构建方法
CN111732657A (zh) * 2020-06-19 2020-10-02 西南大学 用于直接检测蚕丝丝胶蛋白的多肽抗体及制备方法及应用
CN114113376A (zh) * 2021-11-10 2022-03-01 广东一方制药有限公司 僵蚕特征多肽以及僵蚕、僵蚕水提物制品和其他僵蚕制品的鉴别方法
CN115724937A (zh) * 2022-08-26 2023-03-03 西南大学 具有抗菌活性的Sericin3蛋白的重复基序及其应用

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JP2020125274A (ja) * 2019-02-06 2020-08-20 合同会社Ｔｇｓ 繭糸
US20220017918A1 (en) * 2020-07-17 2022-01-20 Kraig Biocraft Laboratories, Inc. Synthesis of Non-Native Proteins in Bombyx Mori by Modifying Sericin Expression
CN112760324B (zh) * 2021-01-18 2023-10-27 西南大学 一种提高家蚕产丝量的方法
US20220372078A1 (en) * 2021-05-20 2022-11-24 Samsung Electronics Co., Ltd. Polypetide, photoresist composition including the same, and method of forming pattern using the same
KR20230083100A (ko) * 2021-12-02 2023-06-09 삼성전자주식회사 폴리펩티드, 이를 포함하는 포토레지스트 조성물 및 이를 이용한 패턴 형성 방법

Citations (2)

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US20050177877A1 (en) * 2002-03-06 2005-08-11 Shingo Hiramatsu Process for producing physiologically active protein using genetically modified silkworm
CN102358902A (zh) * 2011-04-02 2012-02-22 西南大学 家蚕丝素重链基因突变序列及突变的方法和应用

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JP2004135528A (ja) * 2002-10-16 2004-05-13 National Institute Of Agrobiological Sciences カイコを利用したタンパク質の製造方法
FR2852325B1 (fr) * 2003-03-13 2005-06-03 Acide nucleique dirigeant l'expression d'un polypeptide d'interet dans les glandes sericigenes posterieures d'un lepidoptere, et ses applications
JP2008245623A (ja) * 2007-03-30 2008-10-16 Hiroshima Industrial Promotion Organization 改変フィブロインを生産する遺伝子改変カイコ
JP2011103816A (ja) * 2009-11-18 2011-06-02 Neosilk Co Ltd トランスジェニックカイコ
CN104903445B (zh) * 2012-12-25 2017-09-12 国立研究开发法人农业生物资源研究所 后部绢丝腺基因表达单元及具有其的转基因绢丝虫

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US20050177877A1 (en) * 2002-03-06 2005-08-11 Shingo Hiramatsu Process for producing physiologically active protein using genetically modified silkworm
CN102358902A (zh) * 2011-04-02 2012-02-22 西南大学 家蚕丝素重链基因突变序列及突变的方法和应用

Non-Patent Citations (7)

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ANNIE GAREL 等: "structure and organization of the Bombyx mori sericin 1 gene and of the sericins 1 deduced from the sequence of the ser 1b cDNA", 《INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY》 *
BARBARA KLUDKIEWICZ 等: "Structure and expression of the silk adhesive protein Ser2 in Bombyx mori", 《INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY》 *
CONG-ZHAO ZHOU 等: "Fine organization of Bombyx mori fibroin heavy chain gene", 《NUCLEIC ACIDS RESEARCH》 *
RYOKO ISOBE 等: "Antisense and Double-Strand RNA Interference in a Silkworm Ovarian Cell Line", 《JOURNAL OF INSECT BIOTECHNOLOGY AND SERICOLOGY》 *
YOKO TAKASU 等: "Identification and characterization of a novel sericin gene expressed in the anterior middle silk gland of the silkworm Bombyx mori", 《INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY》 *
杜鑫 等: "家蚕丝胶基因研究概况", 《蚕桑通报》 *
汪生鹏 等: "家蚕蚕丝蛋白基因表达调控研究进展", 《安徽农业科学》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471714A (zh) * 2020-05-07 2020-07-31 西南大学 Minos转座子系统介导的真核生物转基因细胞系及构建方法
CN111732657A (zh) * 2020-06-19 2020-10-02 西南大学 用于直接检测蚕丝丝胶蛋白的多肽抗体及制备方法及应用
CN111732657B (zh) * 2020-06-19 2021-12-14 西南大学 用于直接检测蚕丝丝胶蛋白的多肽抗体及制备方法及应用
CN114113376A (zh) * 2021-11-10 2022-03-01 广东一方制药有限公司 僵蚕特征多肽以及僵蚕、僵蚕水提物制品和其他僵蚕制品的鉴别方法
WO2023082512A1 (zh) * 2021-11-10 2023-05-19 广东一方制药有限公司 僵蚕特征多肽以及僵蚕、僵蚕水提物制品和其他僵蚕制品的鉴别方法
CN114113376B (zh) * 2021-11-10 2023-08-29 广东一方制药有限公司 僵蚕特征多肽以及僵蚕、僵蚕水提物制品和其他僵蚕制品的鉴别方法
CN115724937A (zh) * 2022-08-26 2023-03-03 西南大学 具有抗菌活性的Sericin3蛋白的重复基序及其应用
CN115724937B (zh) * 2022-08-26 2023-09-15 西南大学 具有抗菌活性的Sericin3蛋白的重复基序及其应用

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JP6326703B2 (ja) 2018-05-23
WO2015060159A1 (ja) 2015-04-30

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Application publication date: 20160615