CN105670980B - 一种修复重金属污染土壤的微生物菌株的应用 - Google Patents
一种修复重金属污染土壤的微生物菌株的应用 Download PDFInfo
- Publication number
- CN105670980B CN105670980B CN201610248561.2A CN201610248561A CN105670980B CN 105670980 B CN105670980 B CN 105670980B CN 201610248561 A CN201610248561 A CN 201610248561A CN 105670980 B CN105670980 B CN 105670980B
- Authority
- CN
- China
- Prior art keywords
- soil
- heavy metal
- cadmium
- microbial strains
- burkholderia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002689 soil Substances 0.000 title claims abstract description 78
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 42
- 230000000813 microbial effect Effects 0.000 title claims abstract description 28
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 43
- 230000000694 effects Effects 0.000 claims abstract description 28
- 241001453380 Burkholderia Species 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 12
- 241000589513 Burkholderia cepacia Species 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 101710088194 Dehydrogenase Proteins 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 9
- 230000006866 deterioration Effects 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 description 18
- 241000894006 Bacteria Species 0.000 description 17
- 238000002161 passivation Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 8
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 210000001082 somatic cell Anatomy 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000234282 Allium Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 238000009418 renovation Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003900 soil pollution Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241001062472 Stokellia anisodon Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000003802 soil pollutant Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610248561.2A CN105670980B (zh) | 2016-04-20 | 2016-04-20 | 一种修复重金属污染土壤的微生物菌株的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610248561.2A CN105670980B (zh) | 2016-04-20 | 2016-04-20 | 一种修复重金属污染土壤的微生物菌株的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105670980A CN105670980A (zh) | 2016-06-15 |
CN105670980B true CN105670980B (zh) | 2019-11-05 |
Family
ID=56215521
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610248561.2A Active CN105670980B (zh) | 2016-04-20 | 2016-04-20 | 一种修复重金属污染土壤的微生物菌株的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105670980B (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106964647A (zh) * | 2017-06-06 | 2017-07-21 | 临沂大学 | 一种利用复合菌剂修复铅污染土壤的方法 |
CN108130294B (zh) * | 2018-01-15 | 2020-09-29 | 华中农业大学 | 一种用于重金属污染原位修复微生物及应用 |
CN108485998B (zh) * | 2018-02-12 | 2021-08-24 | 暨南大学 | 一株高效活化矿质元素和重金属镉的土壤杆菌t29 |
CN108949609B (zh) * | 2018-05-24 | 2021-10-01 | 苏州科技大学 | 处理重金属污染土壤的微生物菌株及其筛选方法与应用 |
CN110468080B (zh) * | 2019-07-19 | 2024-01-19 | 湖南农业大学 | 促生水稻与降镉的微生物菌剂及其制备方法和使用方法 |
CN110938561B (zh) * | 2019-11-20 | 2022-04-08 | 华南农业大学 | 一株伯克氏菌y-4及其在吸附重金属离子和磺胺类抗生素中的应用 |
CN111676160B (zh) * | 2020-06-16 | 2022-09-23 | 广西中医药大学 | 牛大力内生菌rh5在促进牛大力生长中的应用 |
CN111690564B (zh) * | 2020-06-16 | 2022-08-30 | 广西中医药大学 | 牛大力耐镉内生菌rh5及其应用 |
CN115997791A (zh) * | 2022-12-30 | 2023-04-25 | 华南农业大学 | 洋葱伯克霍尔德氏菌在促进植物耐盐碱及降低重金属镉含量中的应用 |
CN116836895B (zh) * | 2023-09-04 | 2023-11-21 | 山东迈科珍生物科技有限公司 | 一种重金属污染土壤修复菌剂及其制备方法与应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1260282A2 (en) * | 2001-05-16 | 2002-11-27 | "VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK", afgekort "V.I.T.O." | Method for improving phytoremediation treatment of a contaminated medium |
CN106497815A (zh) * | 2015-09-07 | 2017-03-15 | 粮华生物科技(北京)有限公司 | 一种花园伯克霍尔德氏菌、含有该菌的菌剂及其应用和钝化铬的方法 |
-
2016
- 2016-04-20 CN CN201610248561.2A patent/CN105670980B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1260282A2 (en) * | 2001-05-16 | 2002-11-27 | "VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK", afgekort "V.I.T.O." | Method for improving phytoremediation treatment of a contaminated medium |
CN106497815A (zh) * | 2015-09-07 | 2017-03-15 | 粮华生物科技(北京)有限公司 | 一种花园伯克霍尔德氏菌、含有该菌的菌剂及其应用和钝化铬的方法 |
Non-Patent Citations (5)
Title |
---|
Isolation of Burkholderia cepacia JB12 from lead- and cadmium-contaminated soil and its potential in promoting phytoremediation with tall fescue and red clover;Zhong Min Jin et al.;《Can. J. Microbiol.》;20130513(第59期);第449-455页 * |
王荣.耐Pb、Cd细菌耐性机理的研究及其对Pb、Cd生物有效性影响的初探.《中国优秀硕士学位论文全文数据库 工程科技I辑》.2015,中文摘要、第6页第1段. * |
耐Pb、Cd细菌耐性机理的研究及其对Pb、Cd生物有效性影响的初探;王荣;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20150815;中文摘要、第6页第1段 * |
重金属污染土壤原位钝化修复研究进展;王立群 等;《应用生态学报》;20090531;第20卷(第5期);第1214-1222页 * |
长期重金属胁迫对农田土壤微生物生物量、活性和种群的影响;张彦 等;《应用生态学报》;20070731;第18卷(第7期);第1491-1497页 * |
Also Published As
Publication number | Publication date |
---|---|
CN105670980A (zh) | 2016-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105670980B (zh) | 一种修复重金属污染土壤的微生物菌株的应用 | |
CN106119170B (zh) | 修复重金属污染土壤的微生物菌株及其应用 | |
CN106493167B (zh) | 地衣芽孢杆菌和菌剂及它们的应用和钝化重金属的方法 | |
He et al. | Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus | |
CN104531585B (zh) | 一种戴尔福特菌及其应用 | |
CN108865923B (zh) | 用于重金属污染土壤处理的微生物菌株及其筛选方法与应用 | |
CN108949609A (zh) | 处理重金属污染土壤的微生物菌株及其筛选方法与应用 | |
CN108893420A (zh) | 处理重金属污染土壤用微生物菌株及其筛选方法与应用 | |
CN108893419A (zh) | 微生物菌株及其筛选方法与在处理重金属污染土壤中的应用 | |
CN109161497B (zh) | 一种降解黄曲霉毒素的微生物制剂及应用 | |
CN106834269A (zh) | 一种PAEs降解菌的固定化微球及其制备方法和应用 | |
CN106520588B (zh) | 一株铜绿假单胞菌和其菌剂以及它们在钝化铅中的应用 | |
CN110078220B (zh) | 一种利用蓝藻原位修复砷污染高盐碱水的方法及菌株 | |
CN111117909B (zh) | 一种耐多重重金属促植物生长的菌株及其应用 | |
CN103451105B (zh) | 一种高吸附镉的丝状真菌产黄青霉j-5及制备方法和应用 | |
CN113862174B (zh) | 一株抗生素降解菌及其在土壤污染修复中的应用 | |
CN108441455A (zh) | 针对重金属污染土壤处理的微生物菌株及其筛选方法与应用 | |
CN101974448B (zh) | 一种不动杆菌及其在重金属离子生物处置中的应用 | |
CN105861362A (zh) | 一株耐重金属的放线菌菌株及其应用 | |
CN106244501B (zh) | 一株抗锑细菌nxh1及其应用 | |
CN117551557A (zh) | 一株耐低温多功能菌及其在土壤和水体污染修复中的应用 | |
CN102925362A (zh) | 一种尖孢镰刀菌的筛选方法及其应用 | |
CN110343623A (zh) | 一种降解纤维素、吸附镉的草酸青霉菌株及其应用 | |
CN105733966A (zh) | 耐辐射丝状真菌m30及其在吸附镉生物处理中的应用 | |
CN103525746B (zh) | 一种溶藻/藻毒素降解双效工程菌y1及其构建方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160815 Address after: Fengyang high tech Development Zone in Henan Province, Zhengzhou City, 450000 street, No. 2 Applicant after: National Tobacco Quality Supervision Testing Center Applicant after: Guangxi Tobacco Monoploy Bureau, China National Tobacco Corporation Address before: Fengyang high tech Industrial Development Zone, Henan province Zhengzhou City 450000 Street No. 2 Applicant before: National Tobacco Quality Supervision Testing Center |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20190724 Address after: 45 000 1 6, 7, Cuizhu Street, Zhengzhou High-tech Industrial Development Zone, Henan Province Applicant after: National Tobacco Quality Supervision Testing Center Applicant after: Guangxi Tobacco Monoploy Bureau, China National Tobacco Corporation Applicant after: Tsinghua University Address before: Fengyang high tech Development Zone in Henan Province, Zhengzhou City, 450000 street, No. 2 Applicant before: National Tobacco Quality Supervision Testing Center Applicant before: Guangxi Tobacco Monoploy Bureau, China National Tobacco Corporation |
|
GR01 | Patent grant | ||
GR01 | Patent grant |