CN105639191A - Feed additive for protecting intestinal health of hybrid snakehead fish and preparation method of feed additive - Google Patents
Feed additive for protecting intestinal health of hybrid snakehead fish and preparation method of feed additive Download PDFInfo
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- CN105639191A CN105639191A CN201511014972.7A CN201511014972A CN105639191A CN 105639191 A CN105639191 A CN 105639191A CN 201511014972 A CN201511014972 A CN 201511014972A CN 105639191 A CN105639191 A CN 105639191A
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Abstract
The invention discloses a feed additive for protecting intestinal health of a hybrid snakehead fish and a preparation method of the feed additive. The feed additive comprises the following components, by weight part: 25-35 parts of copper-loading montmorillonite, 20-30 parts of mannan oligosaccharide, 15-20 parts of bacillus licheniformis, 10-15 parts of glutamine dipeptide, 7-12 parts of tributyrin, 5-10 parts of origanum vulgare essential oil, and 4-8 parts of yeast nucleic acid. The method comprises: pulverizing the solid raw materials above, sieving the obtained materials through a sieve with 120-140 mesh, mixing the obtained materials with tributyrin and origanum vulgare essential oil to enable a coefficient of variation of the obtained mixture to be no more than 5%, and obtaining the feed additive. The feed additive can promote intestinal growth and development of the hybrid snakehead fish, ensures a normal intestinal structure and function, and optimizes intestinal flora of the hybrid snakehead fish. A normal intestinal micro-ecological environment is ensured, and the ability of digesting and absorbing nutritive substances of the hybrid snakehead fish is improved.
Description
Technical field
The invention belongs to animal feedstuff additive technical field, it is specifically related to a kind of fodder additives and its preparation method of protecting hybridized snakehead fish intestinal health.
Background technology
Intestinal health is the important guarantee of body health, is the main place that fish digest and assimilate nutritive substance; Simultaneously enteron aisle be also the maximum mucosa-immune organ of fish and and the immunization barrier of most fragile, it is the first line of defence that body resists foreign matter. Therefore, intestinal health is the key of healthy fish cultivation, one of enteron aisle main approach that normally fish disease infects; Intestinal health declines, and not only causes body health to decline, and also reduces nutrient digestion specific absorption, does not digest nutritive substance in a large number and enter water body, cause water surrounding " health " to decline, and then reduces fish body health level.
Hybridized snakehead fish is because of its delicious meat, nutritious, and has the pharmaceutical use nourishing and taking good care of, and becomes China and develop economic freshwater breed variety fast. China's snakehead annual production is at about 200,000 tons, and the output value reaches 6,000,000,000 yuan. In recent years, due to the decline of hybridized snakehead fish culture benefit, cause a large amount of uses of plant protein source in its feed, cause hybridized snakehead fish enteron aisle oxidative damage and structure deteriorate; In addition the shortage of hybridized snakehead fish nutritional-physiological, artificial diet compounding process, complete processing and feeding technology research data, and hybridized snakehead fish is high density culture pattern at present, even also mix and feed iced trash fish, these factors all can jeopardize or damage the health of hybridized snakehead fish enteron aisle, and then affect it and grow, restrict the sound development that its industry is sustainable. And then how to protect hybridized snakehead fish intestinal health under cultivating condition, then it is the important channel improving its culture benefit; Therefore develop a kind of can available protecting or regulate the fodder additives of cultured fishes intestinal health to be improve cultured fishes to utilize an important step of artifical compound feed.
Summary of the invention
For prior art above shortcomings; technical problem to be solved by this invention is: how to provide a kind of fodder additives and its preparation method of protecting hybridized snakehead fish intestinal health; make the fodder additives obtained can ensure the health of hybridized snakehead fish enteron aisle; promote enteron aisle digesting and assimilating nutritive substance, the intestine immunity defense function of important protection hybridized snakehead fish body.
In order to solve the problems of the technologies described above; the present invention adopts following technical scheme: a kind of fodder additives protecting hybridized snakehead fish intestinal health; with parts by weight, comprise following component: 25 ~ 35 parts of copper-loaded montmorillonites, 20 ~ 30 parts of mannooligo saccharides, 15 ~ 20 parts of Bacillus licheniformis, 10 ~ 15 parts of glutamine dipeptides, 7 ~ 12 parts of tributyrins, 5 ~ 10 parts of Herba Origani Vulgaris quintessence oils and 4 ~ 8 parts of yeast nucleic acids.
The preparation method of the fodder additives of above-mentioned protection hybridized snakehead fish intestinal health, comprises the steps:
1) component raw material of above-mentioned weight part is taken;
2) copper-loaded montmorillonite, mannosans, Bacillus licheniformis, glutamine dipeptides and the yeast nucleic acid that step 1) are taken are pulverized respectively, and cross 120 ~ 140 order sieves;
3) by step 2) pulverize after copper-loaded montmorillonite, mannosans, Bacillus licheniformis, glutamine dipeptides and yeast nucleic acid be added in the tributyrin and Herba Origani Vulgaris quintessence oil that step 1) takes; mix; make the mixed mixture variation coefficient��5%, obtain the fodder additives of described protection hybridized snakehead fish intestinal health.
The present invention protects the fodder additives of hybridized snakehead fish intestinal health can use as hybridized snakehead fish fodder additives, and addition is the 0.15 ~ 0.30% of feeding quality.
Compared to existing technology, the present invention has following useful effect:
1, the present invention protects the fodder additives of hybridized snakehead fish intestinal health can promote that hybridized snakehead fish enteron aisle grows, ensure that enteron aisle structure and function is normal, fodder additives of the present invention is adopted to feed hybridized snakehead fish, the intestinal mucosa pleat number of hybridized snakehead fish and pleat highly significant higher than control group and business feed group through verification experimental verification; Moreover, fodder additives of the present invention can also optimize the intestinal microflora of hybridized snakehead fish, ensures that intestine microenvironment is normal, feeds the hybridized snakehead fish enteron aisle intestinal bacteria of fodder additives of the present invention through verification experimental verification and Rhodopseudomonas significantly reduces.
2, fodder additives of the present invention can also improve hybridized snakehead fish to the abilities of digestive and absorption of nutritive substance and immunological competence, after verification experimental verification feeds fodder additives of the present invention, the intestines body index of hybridized snakehead fish, Erepsin, enteron aisle and intestinal cells activities of antioxidant enzymes, enteron aisle and liver digestive enzyme activity are all significantly improved.
3, use fodder additives of the present invention can reduce nitrogen phosphorus output, ensure the normal of cultivation water environment, maintain water surrounding in state of health, and then can promote that cultivation hybridized snakehead fish reaches health level.
4, fodder additives of the present invention uses raw materials enjoy stable sources, cheap; And preparation method of the present invention is relatively more simple and easy to operate, is applicable to industrialized production, thus has good market outlook.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail. The implementation case is implemented under premised on the technology of the present invention, now provides detailed enforcement mode and concrete operating process, illustrates that the present invention is creative, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Protect a fodder additives for hybridized snakehead fish intestinal health, comprise following composition: copper-loaded montmorillonite 25kg, mannooligo saccharide 25kg, Bacillus licheniformis 18kg, glutamine dipeptides 12kg, yeast nucleic acid 5kg, tributyrin 9kg and Herba Origani Vulgaris quintessence oil 6kg.
The preparation method of the fodder additives of above-mentioned protection hybridized snakehead fish intestinal health; copper-loaded montmorillonite 25kg, mannooligo saccharide 25kg, Bacillus licheniformis 18kg, glutamine dipeptides 12kg and yeast nucleic acid 5kg are pulverized respectively; after pulverizing, each raw material is crossed 120 order sieves; each raw material after pulverizing and sieving is mixed with tributyrin 9kg and Herba Origani Vulgaris quintessence oil 6kg; make the variation coefficient��5% of mixed mixture, obtain the fodder additives of described protection hybridized snakehead fish intestinal health.
Carrying out the cultivation of hybridized snakehead fish in different zones three place, whole nation testing ground indoor circulation cultivating system, the initial weight of fish is respectively: 16.8 �� 0.67g, 35.3 �� 1.42g and 52.3 �� 2.35g. The feed of control group does not all add the fodder additives of the protection hybridized snakehead fish intestinal health of the present invention; Simultaneously using a kind of business feed (waiting protein level) as business feed group, each control group divides into 4 repetitions; All add 0.15%(mass percent during test group is feeds utilized) product of the present invention, each test group divides into 4 repetitions equally. After the raising experiment of 70 days, in result display test group, the intestinal mucosa pleat number of hybridized snakehead fish, pleat height, mucous membrane pleat height/muscle layer thickness and unit length intestinal mucosa area are all significantly higher than control group and business feed group, and enteron aisle intestinal bacteria and Rhodopseudomonas significantly reduce; Intestines body index improves 18��42%; Erepsin content improves 15��59%; Enteron aisle and intestinal cells activities of antioxidant enzymes improve 7��26%; Enteron aisle and liver digestive enzyme activity improve 14��35%.
Embodiment 2
Protect a fodder additives for hybridized snakehead fish intestinal health, comprise following composition: copper-loaded montmorillonite 35kg, mannooligo saccharide 20kg, Bacillus licheniformis 15kg, glutamine dipeptides 10kg, yeast nucleic acid 6kg, tributyrin 10kg and Herba Origani Vulgaris quintessence oil 5kg.
The preparation method of the fodder additives of above-mentioned protection hybridized snakehead fish intestinal health; copper-loaded montmorillonite 35kg, mannooligo saccharide 20kg, Bacillus licheniformis 15kg, glutamine dipeptides 10kg and yeast nucleic acid 6kg are pulverized respectively; after pulverizing, each raw material is crossed 140 order sieves; each raw material after pulverizing and sieving is mixed with tributyrin 10kg and Herba Origani Vulgaris quintessence oil 5kg; make the variation coefficient��5% of mixed mixture, obtain the fodder additives of described protection hybridized snakehead fish intestinal health.
In the whole nation, different zones three place carries out land for growing field crops culture experiment, and the culture experiment of three place fishes (initial weight 25.6 �� 1.65g) and the experiment of control group carry out on 4 mouthfuls of pools respectively, and the average area on every mouthful of pool is 12 mu. The feed of control group does not all add the fodder additives of the protection hybridized snakehead fish intestinal health of the present invention; Simultaneously using a kind of business feed (waiting protein level) as business feed group; The feed of experimental group adds 0.25%(mass percent) product of the present invention. After the raising experiment of 60 days, in result display experimental group, the intestinal mucosa pleat number of hybridized snakehead fish and pleat highly significant are higher than control group and business feed group, and enteron aisle intestinal bacteria and Rhodopseudomonas significantly reduce. Intestines body index improves 21��39%, and enteron aisle activities of antioxidant enzymes improves 11��32%, and significantly improves digestive enzyme activity.
What finally illustrate is, above embodiment is only in order to illustrate the technical scheme of the present invention and unrestricted, although with reference to better embodiment to invention has been detailed explanation, it will be understood by those within the art that, the technical scheme of the present invention can be modified or equivalent replacement, and not departing from objective and the scope of technical solution of the present invention, it all should be encompassed in the middle of the right of the present invention.
Claims (4)
1. protect the fodder additives of hybridized snakehead fish intestinal health for one kind; it is characterized in that; with parts by weight, comprise following component: 25 ~ 35 parts of copper-loaded montmorillonites, 20 ~ 30 parts of mannooligo saccharides, 15 ~ 20 parts of Bacillus licheniformis, 10 ~ 15 parts of glutamine dipeptides, 7 ~ 12 parts of tributyrins, 5 ~ 10 parts of Herba Origani Vulgaris quintessence oils and 4 ~ 8 parts of yeast nucleic acids.
2. protect the fodder additives of hybridized snakehead fish intestinal health according to claim 1; it is characterized in that; with parts by weight, comprise following component: 25 parts of copper-loaded montmorillonites, 25 parts of mannooligo saccharides, 18 parts of Bacillus licheniformis, 12 parts of glutamine dipeptides, 9 parts of tributyrins, 6 parts of Herba Origani Vulgaris quintessence oils and 5 parts of yeast nucleic acids.
3. protect the preparation method of the fodder additives of hybridized snakehead fish intestinal health for one kind, it is characterised in that, comprise the steps:
1) component raw material of weight part described in claim 1 or 2 is taken;
2) copper-loaded montmorillonite, mannosans, Bacillus licheniformis, glutamine dipeptides and the yeast nucleic acid that step 1) are taken are pulverized respectively, and cross 120 ~ 140 order sieves;
3) by step 2) pulverize after copper-loaded montmorillonite, mannosans, Bacillus licheniformis, glutamine dipeptides and yeast nucleic acid be added in the tributyrin and Herba Origani Vulgaris quintessence oil that step 1) takes; mix; make the mixed mixture variation coefficient��5%, obtain the fodder additives of described protection hybridized snakehead fish intestinal health.
4. protect the preparation method of the fodder additives of hybridized snakehead fish intestinal health according to claim 3, it is characterised in that, step 2) middle 120 orders sieve excessively.
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Cited By (5)
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| CN106387508A (en) * | 2016-09-20 | 2017-02-15 | 江苏农林职业技术学院 | Compound feed for enhancing immunity and disease resistance of hybridized young ophicephalus argus and preparation method thereof |
| CN106689806A (en) * | 2016-12-05 | 2017-05-24 | 浙江海洋大学 | Feed additive improving intestinal microflora of periophthalmus modestus and application thereof |
| CN110463861A (en) * | 2019-08-13 | 2019-11-19 | 河南科技大学 | A kind of feed additive and preparation method improving fancy carp gut barrier function |
| CN110959759A (en) * | 2019-12-16 | 2020-04-07 | 天津纳尔生物科技有限公司 | Origanum essential oil composite coated feed additive and preparation method thereof |
| CN112041427A (en) * | 2018-04-23 | 2020-12-04 | 赢创运营有限公司 | Synbiotic composition |
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| CN105639191B (en) | 2019-08-30 |
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Effective date of registration: 20200326 Address after: 528222 New Industrial Development Zone, Nanhai District, Nanhai District, Guangdong, Foshan Patentee after: GUANGDONG TONGWEI FEED Co.,Ltd. Address before: 400715 Chongqing Road, Beibei District No. 2 Co-patentee before: TONGWEI Co.,Ltd. Patentee before: SOUTHWEST University |
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