CN105616187A - Smearing type intradermal injection capable of directly reaching dermis layer and preparation method thereof - Google Patents
Smearing type intradermal injection capable of directly reaching dermis layer and preparation method thereof Download PDFInfo
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- CN105616187A CN105616187A CN201610080422.3A CN201610080422A CN105616187A CN 105616187 A CN105616187 A CN 105616187A CN 201610080422 A CN201610080422 A CN 201610080422A CN 105616187 A CN105616187 A CN 105616187A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Abstract
The invention discloses smearing type intradermal injection capable of directly reaching the dermis layer and a preparation method thereof. The smearing type intradermal injection comprises, by weight, 10-30 parts of hyaluronic acid and 10-30 parts of collagen. The smearing type intradermal injection has the advantages that the smearing type intradermal injection is high in activity, appropriate in component and reasonable in combination, the smearing type intradermal injection can permeate the epidermis to reach the dermis layer when the smearing type intradermal injection is applied to the face or smeared to the epidermis of the skin, the hyaluronic acid and collagen lost due to aging are injected to allow the skin to absorb and store moisture with the weight being 1000 times of the weight of the skin, the regenerative function of cells is awakened, the withered and fractured cells of the skin are repaired again, the compact, smooth and elastic skin can be achieved again in a short time, effects of removing yellowness and whitening and resisting wrinkles and tightening the skin are achieved, the skin can restore elasticity, be transparent, bright and compact and form a moisture shield, different mechanisms are coordinated to bring an anti-ultraviolet effect into play, ultraviolet permeation can be reduced, the skin damage caused by the ultraviolet can be repaired, dual-protection and a good effect are achieved, and the smearing type intradermal injection has a quite good anti-allergic function.
Description
Technical field
The present invention relates to technical field of skin care, painting type water laser accunputure being specifically related to a kind of through skin corium and preparation method thereof.
Background technology
Skin is the organ that human body is maximum, the 5-15% of gross weight percentage of liveweight, and the gross area is 1.5-2 square metre, and thickness is because of people or different because of position, for 0.5-4 millimeter. Skin covers whole body, and it makes internal various tissue and organ from the invasion and attack of physical property, mechanicalness, chemical and pathogenic microorganism. Skin has the barrier action of two aspects: prevent the loss of internal water, electrolyte and other materials on the one hand; Stop the intrusion of extraneous harmful substance on the other hand. Remain human internal environment stable on, physiology plays important defencive function, skin also assists in the metabolic process of human body simultaneously. As the saying goes " a white screening hundred is ugly ", whitening is all lain in by Chinese very much from ancient times to the present, and whitening is also a kind of maintenance to skin, and the emphasis of the skin-whitening skin protection throughout the year of women especially, the whitening of face is particularly important.
Weakening of the skin tolerance caused owing to selecting improper and frequent life-time service cosmetics and cleaning supplies, can cause skin sensitivity. Consequent symptom includes, it is seen that condition susceptible: erythema and casting off a skin; It is more the supraneural discomfort of subjective sensation, for instance twinge, scorching hot, pruritus, tight etc.
Sensitive-skinned feature: 1, epiderm skin is thin, blood capillary is obvious; 2, skin lacks gloss, cheek easily congested redness; 3, skin is made easily to present unsure state because of seasonal variations; Situation, it may appear that itch, play erythra; 4, cold wind, food, water quality, ultraviolet, synthetic fibers, fragrance it are subject to; Or the impact of material. The physiological period of healthy skin is 28 days, forms the compact texture of marshalling, it is provided that skin protects and moisture-keeping functions fully from basal layer to horny layer. Skin has normal physiological period, and the horny layer horn cell of the skin the superiors can arrange very neat, and lines is extremely careful, oil-water balance. The skin metabolism period staggering of sensitive skin; epidermal structure arrangement is mixed and disorderly; when Skin Cell also immaturity, nature moisturizing factor and intercellular lipid are also manufactured without complete; horny layer just peels off, and keratodermatitis cell arrangement is irregular, and can squeeze toward roll; moisture content within skin easily runs off; do not have the protection of sebum film, excitor substance or allergin easily to invade, cause the internal inflammation of skin.
Because the improper reason making to apply some make up, the people of current sensitivity subhealth state skin quality gets more and more. The product of most of antiallergic senses on the market, is only calm antiinflammatory, and this is helpful to immediate allergic skin, but the conditioning use in fact of sensitive skin is little. Also there are some high-tech biological polypeptides now, such as EGF epidermal growth factor, horny layer can be repaired to a certain extent, but it is expensive, and there is no the effect of immunomodulating anti-inflammatory.
Increase due to the age and the various factors such as seasonal variations, ultraviolet radiation, smoking, operating pressure are big, sitting before computer, television set, contact radioactive substance cause the skin problem of people, as skin corium subside, moisture-retaining capacity declines, xerosis cutis is aging. Therefore, cosmetics have become as the requisite articles for daily use of one of people. Not stimulating skin due to easy to use, safe, can frequently use, facial film has increasingly been active status in Cosmetic Market. A lot, effect is also multifarious for cosmetology product on the market at present, but, some skin care item are in order to pursue the effect on surface for the moment, may some chemical addition agents of improper use, and these additives are excessively used for a long time, the person can be caused serious harm. Skin is exposed in the air of pollution, and skin surface horny layer not only can be made to thicken, also can the colour of skin become obscure, dry without gloss. Some the regular skin protection physical therapy possibilities going to beauty parlor can improve some skin Problems existing, but, if the daily painting type water laser accunputure using gentle effective through skin corium of being in every day should be more the choosing of ideal.
The painting type water laser accunputure of through skin corium is also known as facial treatment essence liquid, and current water laser accunputure is to utilize negative pressure multi pin to inject human body at dermal layer of the skin, skin can cause certain damage, and need patent personnel to be operated, use inconvenience. It is contemplated that invent the painting type water laser accunputure of the through skin corium of a kind of high activity, the scintilla group of skin corium can be penetrated into rapidly, be carrier by biological fiber, these scintillas are made to roll into a ball at normal temperatures and pressures, by deposited face or be applied in skin epidermis, i.e. permeable epidermis, through skin corium.
Summary of the invention
For above shortcomings in prior art, one of the technical problem to be solved is to provide the painting type water laser accunputure of a kind of through skin corium.
The preparation method that the two of the technical problem to be solved are to provide the painting type water laser accunputure of above-mentioned through skin corium.
The present invention seeks to be achieved through the following technical solutions:
The painting type water laser accunputure (being properly termed as again facial treatment essence liquid) of a kind of through skin corium, including following weight parts raw material: 10-30 part hyaluronic acid and 10-30 part collagen protein.
Preferably, the painting type water laser accunputure of a kind of through skin corium, also include following weight parts raw material: 5-10 part glycerol, 1-3 part tocopherol acetas, 1-3 part nicotiamide, 5-10 part squalane, 1-5 part stabilizer, 50-500 part water.
Preferably, the painting type water laser accunputure of a kind of through skin corium, also include following weight parts raw material: 5-10 part glycerol, 1-3 part tocopherol acetas, 1-3 part nicotiamide, 5-10 part squalane, 1-5 part stabilizer, 1-5 part sunscreen, 50-500 part water.
Preferably, described sunscreen is mixed by Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin, described Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin mass ratio be (1-3): (1-3): (1-3).
Preferably, described stabilizer is mixed by carrageenan, trehalose, xylitol, described carrageenan, trehalose, xylitol mass ratio be (1-3): (1-3): (1-3).
Preferably, described hyaluronic acid is that molecular weight is at the daltonian hyaluronic acid of 200-1000.
Preferably, described collagen protein is that molecular weight is at the daltonian collagen protein of 200-1000.
The preparation method that present invention also offers the painting type water laser accunputure of above-mentioned through skin corium, comprises the following steps:
(1) in water vacuum suction that temperature is 80-100 DEG C to emulsifying pot, stabilizer homogenizing will be added under processing condition and process;
(2) when temperature is reduced to 10-40 DEG C, in emulsifying pot, add hyaluronic acid, collagen protein, glycerol, tocopherol acetas, nicotiamide, squalane mix homogeneously, obtain the painting type water laser accunputure of described through skin corium.
Concrete, in the present invention:
Collagen protein, No. CAS: 9064-67-9.
Hyaluronic acid, also known as hyaluronic acid, No. CAS: 9004-61-9.
Tocopherol acetas, No. CAS: 7695-91-2.
Nicotiamide, No. CAS: 98-92-0.
Squalane, No. CAS: 111-01-3.
Folium Symplocoris Caudatae leaf glycosides, No. CAS: 153-18-4, its structural formula is as follows:
Polygonin, No. CAS: 27208-80-6, its structural formula is as follows:
Baicalin, No. CAS: 21967-41-9, its structural formula is as follows:
Carrageenan, No. CAS: 11114-20-8, its structural formula is as follows:
Trehalose, No. CAS: 99-20-7, its structural formula is as follows:
Xylitol, No. CAS: 16277-71-7.
The painting type water laser accunputure of the through skin corium of the present invention is the painting type water laser accunputure of the through skin corium of a kind of high activity, composition is chosen to work as, collocation is rationally, by deposited face or be applied in skin epidermis, i.e. permeable epidermis, through skin corium, inject the hyaluronic acid that human body runs off because of old and feeble, collagen protein, make to absorb in skin and the moisture of 1000 times of the weight of storage own, wake cell regeneration function up, reappear repair skin wither fracture cell, the smooth whippy skin that compacts can be reinvented at short notice, dispel yellow whitening, crease-resistant Firm, recover elasticity, make skin transparent, beautiful, compact, form water conservation barrier, smooth lift skin, stimulate fibroblast proliferation, Cucumber Extract makes skin recover to elastically keep youth, keep skin health, inject vigour into, and play uvioresistant effect from different mechanism is mutually collaborative, not only can reduce ultraviolet passing through, and the skin injury that can ultraviolet be caused is repaired, play double protection, better effects if, and also have extraordinary antianaphylaxis function.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, it is only to presently preferred embodiments of the present invention, the present invention not does the restriction of other forms, and any those skilled in the art are changed to the equal Equivalent embodiments changed possibly also with the technology contents of the disclosure above. Every without departing from the present invention program content, any simple modification following example done according to the technical spirit of the present invention or equivalent variations, all fall within protection scope of the present invention.
Embodiment Raw is introduced:
Collagen protein, No. CAS: 9064-67-9, molecular weight is 500-700 dalton.
Hyaluronic acid, No. CAS: 9004-61-9, molecular weight is 500-700 dalton.
Embodiment 1
Weigh each raw material (weight portion): 20 parts of hyaluronic acid, 20 parts of collagen protein, 6 parts of glycerol, 2 parts of tocopherol acetass, 1.5 parts of nicotiamide, 8 parts of squalanes, 3 parts of stabilizers, 90 parts of water.
Described stabilizer is uniformly mixed is obtained by carrageenan, trehalose, xylitol, described carrageenan, trehalose, xylitol mass ratio be 1:1:1.
The preparation method that present invention also offers the painting type water laser accunputure of above-mentioned through skin corium, comprises the following steps:
(1) in water vacuum suction that temperature is 90 DEG C to emulsifying pot, 2000 revs/min of homogenizing of stabilizer will be added under processing condition and process 5min;
(2) when temperature is reduced to 35 DEG C, adding hyaluronic acid in emulsifying pot, 2000 revs/min of homogenizing process 5min; Adding collagen protein, 2000 revs/min of homogenizing process 5min; Adding glycerol, 2000 revs/min of homogenizing process 5min; Being eventually adding tocopherol acetas, nicotiamide and squalane, 2000 revs/min of homogenizing process 5min; It is down to room temperature, obtains the painting type water laser accunputure of the through skin corium of embodiment 1.
Embodiment 2
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 1 and method, differ only in: described stabilizer is uniformly mixed by carrageenan, trehalose and obtains, described carrageenan, trehalose mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 2.
Embodiment 3
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 1 and method, differ only in: described stabilizer is uniformly mixed by carrageenan, xylitol and obtains, described carrageenan, xylitol mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 3.
Embodiment 4
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 1 and method, differ only in: described stabilizer is uniformly mixed by trehalose, xylitol and obtains, described trehalose, xylitol mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 4.
Embodiment 5
Weigh each raw material (weight portion): 20 parts of hyaluronic acid, 20 parts of collagen protein, 6 parts of glycerol, 2 parts of tocopherol acetass, 1.5 parts of nicotiamide, 8 parts of squalanes, 3 parts of stabilizers, 3 parts of sunscreen, 90 parts of water.
Described sunscreen is uniformly mixed is obtained by Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin, described Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin mass ratio be 1:1:1.
Described stabilizer is uniformly mixed is obtained by carrageenan, trehalose, xylitol, described carrageenan, trehalose, xylitol mass ratio be 1:1:1.
The preparation method that present invention also offers the painting type water laser accunputure of above-mentioned through skin corium, comprises the following steps:
(1) in water vacuum suction that temperature is 90 DEG C to emulsifying pot, 2000 revs/min of homogenizing of stabilizer will be added under processing condition and process 5min;
(2) when temperature is reduced to 35 DEG C, adding hyaluronic acid in emulsifying pot, 2000 revs/min of homogenizing process 5min; Adding collagen protein, 2000 revs/min of homogenizing process 5min; Adding glycerol, 2000 revs/min of homogenizing process 5min; Adding tocopherol acetas, nicotiamide and squalane, 2000 revs/min of homogenizing process 5min; Being eventually adding sunscreen, 2000 revs/min of homogenizing process 5min; It is down to room temperature, obtains the painting type water laser accunputure of the through skin corium of embodiment 5.
Embodiment 6
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 5 and method, differ only in: described sunscreen is uniformly mixed is obtained by Folium Symplocoris Caudatae leaf glycosides, polygonin, described Folium Symplocoris Caudatae leaf glycosides, polygonin mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 6.
Embodiment 7
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 5 and method, differ only in: described sunscreen is uniformly mixed is obtained by Folium Symplocoris Caudatae leaf glycosides, baicalin, described Folium Symplocoris Caudatae leaf glycosides, baicalin mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 7.
Embodiment 8
Prepare the painting type water laser accunputure of through skin corium by the proportioning raw materials of embodiment 5 and method, differ only in: described sunscreen is uniformly mixed by polygonin, baicalin and obtains, described polygonin, baicalin mass ratio be 1:1. Obtain the painting type water laser accunputure of the through skin corium of embodiment 8.
Test case 1
The painting type water laser accunputure of the through skin corium that embodiment 1-8 prepares is carried out defying age test, and test index is skin elasticity and keratodermatitis moisture.
Skin elasticity year skin aging and reduce, therefore skin elasticity is one of important symbol judging skin aging, is the requisite project of efficacy detection of anti-aging cosmetics.
Experimental technique: select healthy, skin normal, without the tested crowd of cosmetic allergic contact dermatitis history, age 20-45 year, wherein male 20, women 20. Within one week, being a course for the treatment of, totally four courses for the treatment of, by facial cleansing every time before using, and each face cleaning time is identical, uses corresponding product after skin dries, and each group uses corresponding product 1 time every day, takes 10g uniform application on the face.
Measure: select Face and cheek as test zone, test after product 10min to be used. Use skin elasticity tester to measure skin elasticity curve, according to the amount of tension of skin and resilience data, the moisturizing of cosmetics and anti-senescence function can be estimated.
Experimental result: skin elasticity test is based on suction and stretches principle, a negative pressure is produced on the surface of tested skin, by in one special test probe of skin inspiration, skin is inhaled into the degree of depth and is recorded by a non-contact optical detection system, measurement result represents typically by an index, unit is 1, and numerical value is more big, illustrates that skin elasticity is more strong. The painting type water light of the through skin corium of the present invention is as shown in table 1 for the impact of skin elasticity.
Table 1: skin elasticity test value table
| Group | 0 week | 1 week | 2 weeks | 3 weeks | 4 weeks |
| Embodiment 1 | 0.485 | 0.548 | 0.641 | 0.679 | 0.710 |
| Embodiment 2 | 0.482 | 0.530 | 0.622 | 0.647 | 0.683 |
| Embodiment 3 | 0.491 | 0.527 | 0.614 | 0.643 | 0.672 |
| Embodiment 4 | 0.487 | 0.531 | 0.627 | 0.651 | 0.695 |
| Embodiment 5 | 0.493 | 0.571 | 0.682 | 0.718 | 0.751 5 --> |
| Embodiment 6 | 0.484 | 0.552 | 0.668 | 0.697 | 0.734 |
| Embodiment 7 | 0.503 | 0.557 | 0.664 | 0.692 | 0.730 |
| Embodiment 8 | 0.489 | 0.555 | 0.653 | 0.687 | 0.724 |
Note: carrying out statistical analysis more afterwards, result shows P < 0.05, and difference has statistical significance.
As can be seen from Table 1, the painting type water laser accunputure of the through skin corium of the present invention can be remarkably reinforced skin elasticity, illustrates that the painting type water laser accunputure of the through skin corium of the present invention has good defying age and effects such as skin of compacting, strengthens elasticity of skin, deep layer repairs skin, makes skin dynamic.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer adopt carrageenan, trehalose, xylitol composite) skin elasticity is substantially better than embodiment 2-4 (stabilizer adopt carrageenan, trehalose, xylitol arbitrarily the two is composite); Comparing embodiment 5 and embodiment 6-8, embodiment 5 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin composite) skin elasticity is substantially better than embodiment 6-8 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin arbitrarily the two is composite).
Moisture is one of important moulding material of epiderm skin horny layer, and during skin aging, horny layer of epidermis is thinning, and in horny layer, natural moisture preserving component content reduces, and skin hydration ability declines, and moisture of skin is lost to be increased; Meanwhile, cell shrinkage Telatrophy, occur in that histological structure and morphological change make skin engender fine wrinkles.
By the mensuration of moisture of skin being reflected to a certain extent the degree of skin aging and effect of Moisturizer.
Experimental technique: select healthy skin, without the tested crowd of cosmetic allergic contact dermatitis history, age 20-45 year, wherein male 20, women 20. Room temperature 25 DEG C �� 1 DEG C; Humidity 50% �� 5%. Cleaning face during test, and everyone face cleaning time is identical, selects Face and cheek 4cm �� 4cm skin as test zone, take 0.8g uniform application on the face after skin dries.
Measure: at Face and cheek (4cm �� 4cm) test zone, after product to be used, 10min tests.
(1) water content of Face and cheek skin is measured with digital skin moisture detector. Use after corresponding product after 1h, 2h, 4h, 6h, survey Face and cheek moisture content of skin, measure and average for three times.
(2) water loss amount of Face and cheek skin is measured with percutaneous moisture loss measuring instrument. Use after corresponding product after 1h, 2h, 4h, 6h, survey Face and cheek moisture of skin windage, measure and average for three times.
Experimental result: use the change of keratodermatitis capacitance before and after product to weigh the change of water content of stratum corneum thus evaluating the moisture-keeping efficacy of cosmetics by testing. Skin keratin moisture is carried out quantification by the method, it is possible to react the change of skin moisture content and favorable reproducibility delicately, is one of method that current moisture-keeping cosmetics efficacy assessments is conventional. This product is as shown in table 2 on the impact of skin moisture content.
Table 2: moisture test value list position: %
| Group | 0h | 1h | 2h | 4h | 6h |
| Embodiment 1 | 30.48 | 62.74 | 60.39 | 57.80 | 56.92 |
| Embodiment 2 | 30.56 | 62.37 | 59.97 | 57.41 | 56.34 |
| Embodiment 3 | 30.49 | 62.48 | 60.08 | 57.49 | 56.43 |
| Embodiment 4 | 30.61 | 62.37 | 60.17 | 57.56 | 56.54 |
| Embodiment 5 | 30.47 | 63.30 | 60.94 | 58.39 | 58.46 |
| Embodiment 6 | 30.72 | 62.87 | 60.48 | 57.94 | 56.93 |
| Embodiment 7 | 30.64 | 62.91 | 60.59 | 58.01 | 57.12 |
| Embodiment 8 | 30.57 | 63.04 | 60.67 | 58.13 | 57.24 |
Note: carrying out statistical analysis more afterwards, result shows P < 0.05, and difference has statistical significance.
As can be seen from Table 2, the painting type water laser accunputure moisturizing of the through skin corium of the present invention is effective, moisture preserving time is long, illustrate that the painting type water laser accunputure of the through skin corium of the present invention can effectively suppress excess oil, balance skin profit, while control oil, also there is moisturizing effect, lasting moisturizing, softening skin.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer adopt carrageenan, trehalose, xylitol composite) moisture is apparently higher than embodiment 2-4 (stabilizer adopt carrageenan, trehalose, xylitol arbitrarily the two is composite); Comparing embodiment 5 and embodiment 6-8, embodiment 5 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin composite) moisture is apparently higher than embodiment 6-8 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin arbitrarily the two is composite).
Test case 2
The painting type water laser accunputure of the through skin corium that embodiment 5-8 prepares is carried out radioprotective test.
Method of testing is with reference to patent application 201410613299.8 description page 5 embodiment 2, [0033rd] section to [0038] section.
The cell used is immortalization epithelial cell (Hacat cell), purchased from consonance cellular resources center, Beijing. Condition of culture is DMEM culture medium (Gibco, the U.S.), the antibacterium of FBS containing 10% (Hyclone, Australia) and 1% and the antibiotic (Invitrogen, the U.S.) of fungus, at 37 DEG C, CO2Concentration is the interior cultivation of incubator of 5%.
Cell set 1 group of blank group, 1 group of negative control group and and 4 groups of test groups, blank group does not carry out UVB (ultraviolet) process without the painting type water laser accunputure of through skin corium yet, without the painting type water laser accunputure of through skin corium in the culture medium of negative control group, 4 groups of test group add the painting type water laser accunputure 0.1mg of the through skin corium of embodiment 5-8 respectively.
Hacat cell is with 4 �� 104Cells/ hole is inoculated in 12 orifice plates, and after cell inoculation 24h, cell sucks culture medium, adds PBS cell once, removes PBS, puts into and carry out UVB process in ultraviolet irradiation case. UVB irradiation bomb wavelength 280-320nm, crest is 312nm. It is 20mJ/cm that UVB processes Hacat cell irradiation dose2��
Hacat cell IL-1 alpha content in culture supernatant after UVB processes makes employment IL-1 ��/IL-1F1DuoSetELISA test kit (R&DSystem, the U.S.) detect, IL-6 content detection uses humanIL-6DuoSetELISA test kit (R&DSystem, the U.S.) carry out, TNF-a content detection uses humanTNF-aDuoSetELISA test kit (R&DSystem, the U.S.) carry out, the same description of method.
The content that each group of test records is respectively divided by the content of negative control group, is multiplied by 100 parts, obtains relative amount. Result of calculation is shown in table 3 below.
Table 3: the painting type water laser accunputure of through skin corium carries out radioprotective test result table
| IL-1 �� (part) | IL-6 (part) | TNF-a (part) | |
| Blank group | 11.2 | 18.0 | 14.3 |
| Negative control group | 100 | 100 | 100 |
| Embodiment 5 | 49.1 | 63.2 | 57.8 |
| Embodiment 6 | 51.2 | 65.8 | 60.4 |
| Embodiment 7 | 50.7 | 65.1 | 59.9 |
| Embodiment 8 | 50.9 | 65.3 | 60.1 |
After UVR process can cause Hacat cell; cell the proinflammatory factor IL-1 ��, IL-6 and TNF-a can be caused to express increase; after culture medium is added the painting type water laser accunputure of the through skin corium using embodiment of the present invention 1-7; significantly reduce the content of IL-1 ��, IL-6 and TNF-a; as can be seen here; it can suppress the inflammatory reaction caused by UVB, and epithelial cell is shielded.
Comparing embodiment 5 and embodiment 6-8, the effect of the inflammatory reaction that system is caused by UVB is substantially better than embodiment 6-8 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin arbitrarily the two is composite) by embodiment 5 (sunscreen adopt Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin composite).
The painting type water laser accunputure of the through skin corium of the present invention is the painting type water laser accunputure of the through skin corium of a kind of high activity, composition is chosen to work as, collocation is rationally, by deposited face or be applied in skin epidermis, i.e. permeable epidermis, through skin corium, inject the hyaluronic acid that human body runs off because of old and feeble, collagen protein, make to absorb in skin and the moisture of 1000 times of the weight of storage own, wake cell regeneration function up, reappear repair skin wither fracture cell, the smooth whippy skin that compacts can be reinvented at short notice, dispel yellow whitening, crease-resistant Firm, recover elasticity, make skin transparent, beautiful, compact, form water conservation barrier, smooth lift skin, stimulate fibroblast proliferation, Cucumber Extract makes skin recover to elastically keep youth, keep skin health, inject vigour into, and play uvioresistant effect from different mechanism is mutually collaborative, not only can reduce ultraviolet passing through, and the skin injury that can ultraviolet be caused is repaired, play double protection, better effects if, and also have extraordinary antianaphylaxis function.
Claims (8)
1. a painting type water laser accunputure for through skin corium, including following weight parts raw material: 10-30 part hyaluronic acid and 10-30 part collagen protein.
2. the painting type water laser accunputure of through skin corium as claimed in claim 1, it is characterized in that, including following weight parts raw material: 10-30 part hyaluronic acid and 10-30 part collagen protein, 5-10 part glycerol, 1-3 part tocopherol acetas, 1-3 part nicotiamide, 5-10 part squalane, 1-5 part stabilizer, 50-500 part water.
3. the painting type water laser accunputure of through skin corium as claimed in claim 2, it is characterized in that, described stabilizer is mixed by carrageenan, trehalose, xylitol, described carrageenan, trehalose, xylitol mass ratio be (1-3): (1-3): (1-3).
4. the painting type water laser accunputure of through skin corium as claimed in claim 2, it is characterized in that, including following weight parts raw material: 10-30 part hyaluronic acid and 10-30 part collagen protein, 5-10 part glycerol, 1-3 part tocopherol acetas, 1-3 part nicotiamide, 5-10 part squalane, 1-5 part stabilizer, 1-5 part sunscreen, 50-500 part water.
5. the painting type water laser accunputure of through skin corium as claimed in claim 4, it is characterized in that, described sunscreen is mixed by Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin, described Folium Symplocoris Caudatae leaf glycosides, polygonin, baicalin mass ratio be (1-3): (1-3): (1-3).
6. the painting type water laser accunputure of the through skin corium as according to any one of claim 1-5, it is characterised in that described hyaluronic acid is that molecular weight is at the daltonian hyaluronic acid of 200-1000.
7. the painting type water laser accunputure of the through skin corium as according to any one of claim 1-5, it is characterised in that described collagen protein is that molecular weight is at the daltonian collagen protein of 200-1000.
8. the preparation method of the painting type water laser accunputure of the through skin corium as described in any one of claim 1-7, it is characterised in that comprise the following steps:
(1) in water vacuum suction that temperature is 80-100 DEG C to emulsifying pot, stabilizer homogenizing will be added under processing condition and process;
(2) when temperature is reduced to 10-40 DEG C, in emulsifying pot, add hyaluronic acid, collagen protein, glycerol, tocopherol acetas, nicotiamide, squalane mix homogeneously, obtain the painting type water laser accunputure of described through skin corium.
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| CN111529442A (en) * | 2020-05-08 | 2020-08-14 | 紫水晶(山西)再生医学科技有限公司 | Water light injection for medical and beauty and preparation method thereof |
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| CN105616187B (en) | 2018-06-19 |
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