CN105616187B - A kind of painting type water laser accunputure of through skin corium and preparation method thereof - Google Patents
A kind of painting type water laser accunputure of through skin corium and preparation method thereof Download PDFInfo
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- CN105616187B CN105616187B CN201610080422.3A CN201610080422A CN105616187B CN 105616187 B CN105616187 B CN 105616187B CN 201610080422 A CN201610080422 A CN 201610080422A CN 105616187 B CN105616187 B CN 105616187B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Abstract
Painting type water laser accunputure the invention discloses a kind of through skin corium and preparation method thereof, the painting type water laser accunputure of the through skin corium include following weight parts raw material：10 30 parts of sodium hyaluronates and 10 30 parts of collagens.The painting type water laser accunputure of the through skin corium of the present invention,It is active high,It is proper into component selections,Collocation is reasonable,By applying face or being applied to skin epidermis,I.e. permeable epidermis,Through skin corium,The sodium hyaluronate that injection human body is lost in due to aging,Collagen,Make interior 1000 times of the moisture absorbed and store weight itself of skin,Wake up cytothesis function,It reappears and repairs skin and wither the cell of fracture,The smooth flexible skin that compacts can be remolded in a short time,It dispels yellow whitening,Crease-resistant Firm,Restore elasticity,Make skin transparent,It is beautiful,It compacts,Form water conservation barrier,And it is mutually cooperateed with from different mechanism and plays uvioresistant effect,The transmission of ultraviolet light can not only be reduced,And the skin injury caused by ultraviolet light can be repaired,Play double protection,Effect is more preferable,And also extraordinary antianaphylaxis function.
Description
Technical field
The present invention relates to technical field of skin care, and in particular to a kind of painting type water laser accunputure of through skin corium and its preparation
Method.
Background technology
Skin is the organ of human body maximum, the 5-15% of total weight percentage of liveweight, and the gross area is 1.5-2 square metres, thickness because
People or due to position it is different, be 0.5-4 millimeters.Skin covers whole body, it makes various in vivo organize with organ from physical, mechanical
Property, chemically with the invasion of pathogenic microorganism.There are two the barrier actions of aspect for skin tool：On the one hand internal water is prevented,
The loss of electrolyte and other substances；On the other hand the intrusion of extraneous harmful substance is prevented.Remain the stabilization of human internal environment
On, important defencive function is physiologically being played, while skin also assists in the metabolic process of human body.It as the saying goes " white a screening hundred
It is ugly ", Chinese lie in whitening very much from ancient times to the present, and whitening is also a kind of maintenance to skin, and skin-whitening is even more women one
The emphasis of year four seasons skin care, the whitening of face are particularly important.
Skin tolerance subtracts caused by selecting improper and frequent long-time service cosmetics and cleaning supplies
It is weak, skin sensitivity can be caused.Resulting symptom includes, it is seen that condition susceptible：Erythema and husking；It is more subjective sense
Feel supraneural discomfort, such as shouting pain, scorching hot, itch, tight etc..
The feature of sensitive skin：1st, skin epidermis is thin, and capilary is apparent；2nd, it is easily congested red and swollen to lack gloss, cheek for skin；
3rd, so that unsure state is easily presented in skin due to seasonal variations；Situation, it may appear that itch, play fash；4th, easily by cold wind,
Food, water quality, ultraviolet light, synthetic fibers, fragrance；Or the influence of substance.The physiological period of healthy skin is 28 days, from substrate
Layer forms the compact texture of marshalling to cuticula, provides skin adequately protection and moisture-keeping functions.Skin possesses normally
Physiological period, the cuticula horn cell of skin top layer can arrange very neatly, and lines is extremely careful, oil-water balance.Sensitive flesh
The skin metabolism period staggering of skin, epidermal structure arrangement is mixed and disorderly, Skin Cell also prematurity, natural moisturizing factor and iuntercellular fat
When matter is also manufactured without complete, cuticula just peels off, and keratoderma cell arrangement is irregular, and can up roll up crowded, skin
Internal moisture content is easily lost in, and without the protection of sebum film, excitor substance or allergin easily invade, and causes to send out inside skin
It is scorching.
Because improper the reason of using cosmetics, the people of the skin quality of sensibility inferior health at present is more and more.On the market mostly
The product of number antiallergic senses, anti-inflammatory of only calming, this is helpful to immediate allergic skin, but to the conditioning of sensitive its
At practicality less.There are also some high-tech biological polypeptides, such as EGF epidermal growth factor, can repair to a certain extent
Cuticula, but its is expensive, and without the effect of immunological regulation anti-inflammatory.
Due to the age increase and seasonal variations, ultraviolet light irradiation, smoke, operating pressure is big, before computer, television set long
It sits, contact the skin problem that the various factors such as radioactive substance cause people, as skin corium collapses, moisture-retaining capacity declines, skin
Skin dries aging.Therefore, cosmetics have become a kind of essential articles for daily use of people.Due to the use of convenient, safety
Skin is not stimulated, can frequently be used, facial mask is increasingly active status in Cosmetic Market.Skin care on the market at present
There are many beauty product, and effect is also multifarious, and but, some skin care item, may be improper in order to pursue the effect on a period of time surface
Using some chemical addition agents, and these additives are excessively used for a long time, and human body can be caused to seriously endanger.Skin is exposed to
Have in the air of pollution, skin surface cuticula can not only thickeied, can also the colour of skin become obscure, it is dry without gloss.It goes
The regular skin care physical therapy of some of beauty parlor may can improve some skins there are the problem of, but, if it is daily to be in daily
The painting type water laser accunputure for using mild effective through skin corium more should be the choosing of ideal.
The painting type water laser accunputure of through skin corium is also known as facial treatment essence liquid, and water laser accunputure is existed using negative pressure multi needles at present
Dermal layer of the skin injects human body, and skin can be caused centainly to damage, and patent personnel is needed to be operated, inconvenient for use.This
Invention is intended to invent a kind of high activity and goes directly the painting type water laser accunputure of skin corium, can penetrate into the scintilla group of skin corium rapidly,
It is carrier by biological fiber, makes these scintillas group at normal temperatures and pressures, by applies face or being applied to skin epidermis, you can
Epidermis is permeated, go directly skin corium.
Invention content
For above-mentioned deficiency in the prior art, the first technical problem to be solved by the present invention is to provide a kind of straight
Up to the painting type water laser accunputure of skin corium.
The second technical problem to be solved by the present invention is to provide the preparation of the painting type water laser accunputure of above-mentioned through skin corium
Method.
The present invention seeks to what is be achieved through the following technical solutions：
The painting type water laser accunputure (and being properly termed as facial treatment essence liquid) of a kind of through skin corium, including following weight parts original
Material：10-30 parts of sodium hyaluronates and 10-30 parts of collagens.
Preferably, the painting type water laser accunputure of a kind of through skin corium, further includes following weight parts raw material：5-10 parts of glycerine,
1-3 parts of tocopherol acetates, 1-3 parts of niacinamide, 5-10 parts of saualanes, 1-5 parts of stabilizers, 50-500 parts of water.
Preferably, the painting type water laser accunputure of a kind of through skin corium, further includes following weight parts raw material：5-10 parts of glycerine,
1-3 parts of tocopherol acetates, 1-3 parts of niacinamide, 5-10 parts of saualanes, 1-5 parts of stabilizers, 1-5 portions of sun-screening agents, 50-500 parts
Water.
Preferably, the sun-screening agent is mixed by rue leaf glycosides, polygonin, scutelloside, the rue leaf glycosides, giant knotweed
Glycosides, scutelloside mass ratio be (1-3)：(1-3)：(1-3).
Preferably, the stabilizer is mixed by carragheen, trehalose, xylitol, the carragheen, trehalose,
The mass ratio of xylitol is (1-3)：(1-3)：(1-3).
Preferably, the sodium hyaluronate is sodium hyaluronate of the molecular weight in 200-1000 dalton.
Preferably, the collagen is collagen of the molecular weight in 200-1000 dalton.
The present invention also provides the preparation methods of the painting type water laser accunputure of above-mentioned through skin corium, include the following steps：
(1) the water vacuum that temperature is 80-100 DEG C is pumped into emulsification pot, stabilizer homogeneous is added under processing condition
Processing；
(2) when temperature is reduced to 10-40 DEG C, sodium hyaluronate, collagen, glycerine, tocopherol second are added in into emulsification pot
Acid esters, niacinamide, saualane are uniformly mixed the painting type water laser accunputure to get the through skin corium.
Specifically, in the present invention：
Collagen, No. CAS：9064-67-9.
Sodium hyaluronate, also known as hyaluronic acid, No. CAS：9004-61-9.
Tocopherol acetate, No. CAS：7695-91-2.
Niacinamide, No. CAS：98-92-0.
Saualane, No. CAS：111-01-3.
Rue leaf glycosides, No. CAS：153-18-4, structural formula are as follows：
Polygonin, No. CAS：27208-80-6, structural formula are as follows：
Scutelloside, No. CAS：21967-41-9, structural formula are as follows：
Carragheen, No. CAS：11114-20-8, structural formula are as follows：
Trehalose, No. CAS：99-20-7, structural formula are as follows：
Xylitol, No. CAS：16277-71-7.
The painting type water laser accunputure of the through skin corium of the present invention is that a kind of high activity is gone directly the painting type water laser accunputure of skin corium, into
Component selections are proper, and collocation is reasonable, by applying face or being applied to skin epidermis, you can infiltration epidermis, go directly skin corium, injects people
Sodium hyaluronate that body is lost in due to aging, collagen make 1000 times of moisture of weight itself is absorbed and stored in skin, wake up
Cytothesis function reappears and repairs skin and wither the cell of fracture, can remold in a short time compact it is smooth flexible
Skin, yellow whitening of dispelling, crease-resistant Firm, restore elasticity, make skin it is transparent, it is beautiful, compact, formed water conservation barrier, smoothly lift flesh
Skin stimulates fibroblast proliferation, and Cucumber Extract makes skin recovery elastically keep youth, keeps skin health, inject vigour into, and
It is mutually cooperateed with from different mechanism and plays uvioresistant effect, can not only reduce the transmission of ultraviolet light, but also can be to ultraviolet light institute
Caused by skin injury repaired, rise double protection, effect is more preferable, and also extraordinary antianaphylaxis function.
Specific embodiment
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention
Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents be changed to the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, according to the present invention
Any simple modification for being made to following embodiment of technical spirit or equivalent variations, all fall in protection scope of the present invention.
Raw material introduction in embodiment：
Collagen, No. CAS：9064-67-9, molecular weight is in 500-700 dalton.
Sodium hyaluronate, No. CAS：9004-61-9, molecular weight is in 500-700 dalton.
Embodiment 1
Weigh each raw material (parts by weight)：20 parts of sodium hyaluronates, 20 parts of collagens, 6 parts of glycerine, 2 parts of tocopherol acetates,
1.5 parts of niacinamide, 8 parts of saualanes, 3 parts of stabilizers, 90 parts of water.
The stabilizer is uniformly mixed to obtain by carragheen, trehalose, xylitol, the carragheen, trehalose,
The mass ratio of xylitol is 1：1：1.
The present invention also provides the preparation methods of the painting type water laser accunputure of above-mentioned through skin corium, include the following steps：
(1) water vacuum at a temperature of 90 °C is pumped into emulsification pot, 2000 revs/min of stabilizer is added under processing condition
Homogenization 5min；
(2) when temperature is reduced to 35 DEG C, sodium hyaluronate, 2000 revs/min of homogenization 5min are added in into emulsification pot；It adds in
Collagen, 2000 revs/min of homogenization 5min；Add in glycerine, 2000 revs/min of homogenization 5min；It is eventually adding tocopherol
Acetic acid esters, niacinamide and saualane, 2000 revs/min of homogenization 5min；It is down to through skin corium of the room temperature to get embodiment 1
Painting type water laser accunputure.
Embodiment 2
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 1, is differed only in：It is described
Stabilizer be uniformly mixed to obtain by carragheen, trehalose, the carragheen, trehalose mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 2.
Embodiment 3
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 1, is differed only in：It is described
Stabilizer be uniformly mixed to obtain by carragheen, xylitol, the carragheen, xylitol mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 3.
Embodiment 4
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 1, is differed only in：It is described
Stabilizer be uniformly mixed to obtain by trehalose, xylitol, the trehalose, xylitol mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 4.
Embodiment 5
Weigh each raw material (parts by weight)：20 parts of sodium hyaluronates, 20 parts of collagens, 6 parts of glycerine, 2 parts of tocopherol acetates,
1.5 parts of niacinamide, 8 parts of saualanes, 3 parts of stabilizers, 3 portions of sun-screening agents, 90 parts of water.
The sun-screening agent is uniformly mixed to obtain by rue leaf glycosides, polygonin, scutelloside, the rue leaf glycosides, tiger
Cane glycosides, scutelloside mass ratio be 1：1：1.
The stabilizer is uniformly mixed to obtain by carragheen, trehalose, xylitol, the carragheen, trehalose,
The mass ratio of xylitol is 1：1：1.
The present invention also provides the preparation methods of the painting type water laser accunputure of above-mentioned through skin corium, include the following steps：
(1) water vacuum at a temperature of 90 °C is pumped into emulsification pot, 2000 revs/min of stabilizer is added under processing condition
Homogenization 5min；
(2) when temperature is reduced to 35 DEG C, sodium hyaluronate, 2000 revs/min of homogenization 5min are added in into emulsification pot；It adds in
Collagen, 2000 revs/min of homogenization 5min；Add in glycerine, 2000 revs/min of homogenization 5min；Add in tocopheryl acetate
Ester, niacinamide and saualane, 2000 revs/min of homogenization 5min；It is eventually adding sun-screening agent, 2000 revs/min of homogenization 5min；
It is down to painting type water laser accunputure of the room temperature to get the through skin corium of embodiment 5.
Embodiment 6
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 5, is differed only in：It is described
Sun-screening agent be uniformly mixed to obtain by rue leaf glycosides, polygonin, the rue leaf glycosides, polygonin mass ratio be 1：1.I.e.
Obtain the painting type water laser accunputure of the through skin corium of embodiment 6.
Embodiment 7
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 5, is differed only in：It is described
Sun-screening agent be uniformly mixed to obtain by rue leaf glycosides, scutelloside, the rue leaf glycosides, scutelloside mass ratio be 1：1.I.e.
Obtain the painting type water laser accunputure of the through skin corium of embodiment 7.
Embodiment 8
The painting type water laser accunputure of through skin corium is prepared by the raw material proportioning and method of embodiment 5, is differed only in：It is described
Sun-screening agent be uniformly mixed to obtain by polygonin, scutelloside, the polygonin, scutelloside mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 8.
Test case 1
Anti-aging test is carried out to the painting type water laser accunputure for the skin corium that goes directly made from embodiment 1-8, test index is skin
Skin elasticity and keratoderma moisture.
Skin elasticity year skin aging and reduce, therefore skin elasticity is the one of the important signs that for judging skin aging, be
The effect of anti-aging cosmetics, detects essential project.
Experimental method：Selection is healthy, skin is normal, without the tested crowd of cosmetic allergic contact dermatitis history, age 20-45 Sui, wherein man
Property 20, women 20.One week is as a treatment course, totally four courses for the treatment of, every time using preceding by facial cleansing, and the face cleaning time every time
It is identical, corresponding product is used daily 1 time, 10g is taken uniformly to be applied on the face after skin dries using corresponding product, each group.
It measures：Face and cheek is selected as test zone, is tested after product 10min to be used.It is surveyed using skin elasticity
It tries instrument and measures skin elasticity curve, it, can be to the moisturizing of cosmetics and anti-aging work(according to the amount of tension of skin and resilience data
It can be assessed.
Experimental result：Skin elasticity test is to be based on suction and stretch principle, and generating one on the surface of tested skin bears
Pressure, by one special test probe of skin inspiration, skin is inhaled into depth and is surveyed by a non-contact optical detection system
, measurement result usually represents that unit 1, numerical value is bigger with an index, illustrates that skin elasticity is stronger.The present invention is through
Influence of the painting type water laser accunputure of skin corium to skin elasticity is as shown in table 1.
Table 1：Skin elasticity test value table
| Group | 0 week | 1 week | 2 weeks | 3 weeks | 4 weeks |
| Embodiment 1 | 0.485 | 0.548 | 0.641 | 0.679 | 0.710 |
| Embodiment 2 | 0.482 | 0.530 | 0.622 | 0.647 | 0.683 |
| Embodiment 3 | 0.491 | 0.527 | 0.614 | 0.643 | 0.672 |
| Embodiment 4 | 0.487 | 0.531 | 0.627 | 0.651 | 0.695 |
| Embodiment 5 | 0.493 | 0.571 | 0.682 | 0.718 | 0.751 |
| Embodiment 6 | 0.484 | 0.552 | 0.668 | 0.697 | 0.734 |
| Embodiment 7 | 0.503 | 0.557 | 0.664 | 0.692 | 0.730 |
| Embodiment 8 | 0.489 | 0.555 | 0.653 | 0.687 | 0.724 |
Note：After carry out statistical analysis, the results showed that P ＜ 0.05, difference have statistical significance.
As it can be seen from table 1 skin elasticity, explanation can be remarkably reinforced in the painting type water laser accunputure of the through skin corium of the present invention
The painting type water laser accunputure of the through skin corium of the present invention has good anti-aging and the skin and other effects that compacts, and enhances elasticity of skin,
Deep layer repairs skin, makes skin dynamic.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer is using carragheen, trehalose, xylitol compounding) skin
Skin elasticity is substantially better than embodiment 2-4 (stabilizer is using carragheen, trehalose, the arbitrary the two compounding of xylitol)；Compare implementation
Example 5 and embodiment 6-8, embodiment 5 (sun-screening agent is using rue leaf glycosides, polygonin, scutelloside compounding) skin elasticity are substantially better than
Embodiment 6-8 (sun-screening agent is using rue leaf glycosides, polygonin, the arbitrary the two compounding of scutelloside).
Moisture is one of important moulding substance of skin epidermis cuticula, and horny layer of epidermis is thinning during skin aging, cutin
Natural moisture preserving component content is reduced in layer, and skin hydration ability declines, and moisture of skin, which is lost, to be increased；Meanwhile cell shrinkage tissue
There is histological structure and morphological change and skin is made to gradually appear fine wrinkles in atrophy.
It can reflect the degree and Moisturizer of skin aging to a certain extent by measure to moisture of skin
Effect.
Experimental method：Select healthy skin, no tested crowd of cosmetic allergic contact dermatitis history, wherein age 20-45 Sui, male 20
Position, women 20.25 DEG C ± 1 DEG C of room temperature；Humidity 50% ± 5%.Face is cleaned during experiment, and everyone face cleaning time is identical, treats
Skin selects Face and cheek 4cm × 4cm skins 0.8g to be taken uniformly to be applied on the face as test zone after drying.
It measures：10min is tested after Face and cheek (4cm × 4cm) test zone, product to be used.
(1) with digital skin moisture detector come the water content of aspect of measure skin of cheek.Using 1h after corresponding product,
After 2h, 4h, 6h, Face and cheek moisture content of skin is surveyed, measure is averaged three times.
(2) with percutaneous moisture loss measuring instrument come the water loss amount of aspect of measure skin of cheek.Use corresponding product
Afterwards after 1h, 2h, 4h, 6h, Face and cheek moisture of skin windage is surveyed, measure is averaged three times.
Experimental result：By testing water content of stratum corneum is weighed using the variation of keratoderma capacitance before and after product
Variation so as to evaluate the moisture-keeping efficacy of cosmetics.The method carries out quantification to skin keratin moisture, can be sensitive
Ground reacts the variation of skin moisture content, and favorable reproducibility, is one of current common method of moisture-keeping cosmetics efficacy assessments.This
Influence of the product to skin moisture content is as shown in table 2.
Table 2：Moisture test value list position：%
| Group | 0h | 1h | 2h | 4h | 6h |
| Embodiment 1 | 30.48 | 62.74 | 60.39 | 57.80 | 56.92 |
| Embodiment 2 | 30.56 | 62.37 | 59.97 | 57.41 | 56.34 |
| Embodiment 3 | 30.49 | 62.48 | 60.08 | 57.49 | 56.43 |
| Embodiment 4 | 30.61 | 62.37 | 60.17 | 57.56 | 56.54 |
| Embodiment 5 | 30.47 | 63.30 | 60.94 | 58.39 | 58.46 |
| Embodiment 6 | 30.72 | 62.87 | 60.48 | 57.94 | 56.93 |
| Embodiment 7 | 30.64 | 62.91 | 60.59 | 58.01 | 57.12 |
| Embodiment 8 | 30.57 | 63.04 | 60.67 | 58.13 | 57.24 |
Note：After carry out statistical analysis, the results showed that P ＜ 0.05, difference have statistical significance.
As can be seen from Table 2, the painting type water laser accunputure moisturizing effect of the through skin corium of the present invention is good, moisture preserving time is long, says
The painting type water laser accunputure of the bright through skin corium of the present invention can effectively inhibit excess oil, balance skin grease, while oil-control
Also there is moisturizing effect, lasting moisturizing, softening skin.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer is using carragheen, trehalose, xylitol compounding) water
Point content is apparently higher than embodiment 2-4 (stabilizer is using both the arbitrary compounding of carragheen, trehalose, xylitol)；Compare implementation
Example 5 and embodiment 6-8, embodiment 5 (sun-screening agent is using rue leaf glycosides, polygonin, scutelloside compounding) moisture are apparently higher than
Embodiment 6-8 (sun-screening agent is using rue leaf glycosides, polygonin, the arbitrary the two compounding of scutelloside).
Test case 2
Radiation protection test is carried out to the painting type water laser accunputure for the skin corium that goes directly made from embodiment 5-8.
Test method is with reference to patent application 201410613299.8 specification embodiment 2 of page 5, and [0033] section is extremely
[0038] section.
The cell used is immortalizes epithelial cell (Hacat cells), purchased from Beijing consonance cell resource center.Cultivate item
Part is DMEM culture mediums (Gibco, the U.S.), antibacterium and fungi containing 10% FBS (Hyclone, Australia) and 1%
Antibiotic (Invitrogen, the U.S.), in 37 DEG C, CO2It is cultivated in a concentration of 5% incubator.
Cell set 1 group of blank control group, 1 group of negative control group and with 4 groups of test groups, blank control group is not added through true
The painting type water laser accunputure of cortex is also handled without UVB (ultraviolet light), and through corium is not added on the culture medium of negative control group
The painting type water laser accunputure of layer, 4 groups of test groups add the painting type water laser accunputure 0.1mg of the through skin corium of embodiment 5-8 respectively.
Hacat cells are with 4 × 104Cells/ holes are inoculated in 12 orifice plates, cell inoculation for 24 hours after, cell sucks culture medium,
It is primary to add in PBS cleaning cells, removes PBS, is put into progress UVB processing in ultraviolet irradiation case.UVB irradiation source wavelengths 280-
320nm, wave crest 312nm.UVB processing Hacat cells irradiation doses are 20mJ/cm2。
IL-1 alpha content user IL-1 α/IL-1F1DuoSet of the Hacat cells after UVB is handled in culture supernatant
ELISA kit (R＆D System, the U.S.) is detected, and IL-6 content detections are tried using humanIL-6DuoSet ELISA
Agent box (R＆D System, the U.S.) carries out, and TNF-a content detections use human TNF-aDuoSet ELISA kits (R＆D
System, the U.S.) it carries out, the same specification of method.
The content difference divided by the content of negative control group that each group experiment is measured, are multiplied by 100 parts, obtain relative amount.
Result of calculation see the table below 3.
Table 3：The painting type water laser accunputure of through skin corium carries out radiation protection test result table
| IL-1 α (part) | IL-6 (part) | TNF-a (part) | |
| Blank control group | 11.2 | 18.0 | 14.3 |
| Negative control group | 100 | 100 | 100 |
| Embodiment 5 | 49.1 | 63.2 | 57.8 |
| Embodiment 6 | 51.2 | 65.8 | 60.4 |
| Embodiment 7 | 50.7 | 65.1 | 59.9 |
| Embodiment 8 | 50.9 | 65.3 | 60.1 |
After UVR processing can cause Hacat cells, cell anticusp inflammation factor IL-1 α, IL-6 and TNF-a can be caused to express and increase
Add, in culture medium after painting type water laser accunputure of the addition using the through skin corium of 1-7 of the embodiment of the present invention, significantly reduce IL-1
The content of α, IL-6 and TNF-a, it can be seen that, the inflammatory reaction as caused by UVB can be inhibited, protection is risen to epithelial cell and is made
With.
Comparing embodiment 5 and embodiment 6-8, embodiment 5 (sun-screening agent is using rue leaf glycosides, polygonin, scutelloside compounding)
To system the inflammatory reaction as caused by UVB it is with obvious effects better than embodiment 6-8 (sun-screening agent using rue leaf glycosides, polygonin, radix scutellariae
The arbitrary the two compounding of glycosides).
The painting type water laser accunputure of the through skin corium of the present invention is that a kind of high activity is gone directly the painting type water laser accunputure of skin corium, into
Component selections are proper, and collocation is reasonable, by applying face or being applied to skin epidermis, you can infiltration epidermis, go directly skin corium, injects people
Sodium hyaluronate that body is lost in due to aging, collagen make 1000 times of moisture of weight itself is absorbed and stored in skin, wake up
Cytothesis function reappears and repairs skin and wither the cell of fracture, can remold in a short time compact it is smooth flexible
Skin, yellow whitening of dispelling, crease-resistant Firm, restore elasticity, make skin it is transparent, it is beautiful, compact, formed water conservation barrier, smoothly lift flesh
Skin stimulates fibroblast proliferation, and Cucumber Extract makes skin recovery elastically keep youth, keeps skin health, inject vigour into, and
It is mutually cooperateed with from different mechanism and plays uvioresistant effect, can not only reduce the transmission of ultraviolet light, but also can be to ultraviolet light institute
Caused by skin injury repaired, rise double protection, effect is more preferable, and also extraordinary antianaphylaxis function.
Claims (3)
1. the painting type water laser accunputure of a kind of through skin corium, which is characterized in that including following weight parts raw material：10-30 parts of glass urine
Acid and 10-30 parts of collagens, 5-10 parts of glycerine, 1-3 parts of tocopherol acetates, 1-3 parts of niacinamide, 5-10 parts of saualanes, 1-5
Part stabilizer, 1-5 portions of sun-screening agents, 50-500 parts of water；
The stabilizer is mixed by carragheen, trehalose, xylitol, the carragheen, trehalose, xylitol quality
Than for (1-3)：(1-3)：(1-3)；
The sun-screening agent is mixed by rue leaf glycosides, polygonin, scutelloside, the rue leaf glycosides, polygonin, scutelloside
Mass ratio is (1-3)：(1-3)：(1-3).
2. the painting type water laser accunputure of through skin corium as described in claim 1, which is characterized in that the sodium hyaluronate is molecule
Measure the sodium hyaluronate in 200-1000 dalton.
3. the painting type water laser accunputure of through skin corium as described in claim 1, which is characterized in that the collagen is divides
Son amount is in the collagen of 200-1000 dalton.
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