CN104856892B - Kinds of cosmetic or skin care preparation and preparation method enriched in growth factors - Google Patents

Kinds of cosmetic or skin care preparation and preparation method enriched in growth factors Download PDF

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CN104856892B
CN104856892B CN201410643201.3A CN201410643201A CN104856892B CN 104856892 B CN104856892 B CN 104856892B CN 201410643201 A CN201410643201 A CN 201410643201A CN 104856892 B CN104856892 B CN 104856892B
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skin
amount
growth factor
oil
growth factors
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CN104856892A (en
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黄亚东
陈玉容
项琪
贝煜
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广州远想生物科技有限公司
广州暨南大学医药生物技术研究开发中心
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Abstract

本发明涉及化妆品技术领域,尤其涉及种富含生长因子的化妆品或护肤品制剂及其制备方法。 The present invention relates to the field of cosmetics, in particular cosmetic or skin care preparation and preparation method involves species rich in growth factors. 本发明提供种主要成分为细胞生长因子,小分子胜肽,水、油脂、表面活性剂、保湿剂、抗氧化剂、防腐剂组成的富含生长因子的化妆品或者护肤品制剂及其制备方法。 The present invention provides major components in cosmetics or skin care products Growth and preparation method thereof enriched in growth factors factor, a small molecule peptide, water, oil, surfactant, humectant, an antioxidant, a preservative for cells. 该组合物制剂的配方间相互搭配不仅能加速皮肤细胞的新陈代谢,修复断裂的浅表皮层纤维细胞,对皮肤创面有突出的快速修复功能,而且还具有美白祛皱、镇静抗敏、柔肤、抗皱、增白、清爽、抗衰老、抗辐射、预防和减轻色斑等多种功效。 Formulations of the composition between formulation with each other not only to accelerate the metabolism of skin cells, skin fibroblasts superficial repair broken, projecting rapid wound repair function of the skin, but also has anti-wrinkle whitening, sedation allergy, Skin, anti-wrinkle, whitening, refreshing, anti-aging, anti-radiation, prevention and mitigation of pigmentation and other effects.

Description

一种富含生长因子的化妆品或护肤品制剂及其制备方法 A rich source of growth factors cosmetic or skin care preparation and preparation method

技术领域 FIELD

[0001] 本发明涉及化妆品技术领域,尤其涉及一种富含生长因子的化妆品或护肤品制剂及其制备方法。 [0001] The present invention relates to the field of cosmetics, in particular, it relates to a cosmetic or skin care preparations and a process for preparing enriched in growth factors.

背景技术 Background technique

[0002] 现代科学技术的飞速发展和广泛应用,给美容化妆品行业带来了全新的发展机遇,护肤品已经从化学美容、植物美容向生物美容、基因美容发展。 [0002] The rapid development of modern science and technology and extensive application to the cosmetics industry has brought new development opportunities, beauty skin care products has grown from chemical, biological plant beauty to beauty, the development of gene beauty. 生物护肤品的主要成分生物活性多肽大部分是细胞生长因子,它们在体内含量极微,但生物活性极高,对多种细胞生理功能和代谢活动发挥生物调节作用,直接或间接地影响多种类型细胞的生长、分裂、分化、增殖和迀移,在美容护肤、整形外科、烧伤溃疡以及各种皮肤病的伤口修复与愈合中有重要作用。 The main component of most biological skincare biologically active polypeptide is a cell growth factors, their minimal levels in the body, but the high biological activity, a variety of physiological functions and metabolic activity of cells play a role in biological regulation, directly or indirectly affect more the type of cell growth, division, differentiation, proliferation, and Gan shift skin in cosmetic, plastic surgery, burns, ulcers and various skin conditions healed wound repair and plays an important role.

[0003] 主要的生长因子包括成纤维细胞生长因子(Fibroblast growth factors,FGFs), 人表皮细胞生长因子(human epidermal growth factor,hEGF),角质细胞生长因子(keratinocyte growth factor,KGF)以及血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)等。 [0003] The main growth factors include fibroblast growth factors (Fibroblast growth factors, FGFs), human epidermal growth factor (human epidermal growth factor, hEGF), keratinocyte growth factor (keratinocyte growth factor, KGF) and vascular endothelial cells growth factor (vascular endothelial growth factor, VEGF) and the like. 其中FGFs近年来备受关注,FGFs目前有23个成员,其中比较重要的主要包括酸性成纤维细胞生长因子(aFGF)和碱性成纤维细胞生长因子(bFGF)。 Wherein FGFs concern in recent years, there are 23 members of the FGFs, including the more important of acidic fibroblast growth factor (of aFGF) and basic fibroblast growth factor (bFGF).

[0004] 生长因子中EGF能促进表皮细胞生长,修复表皮,FGF能促进成纤维细胞生长,加速胶原生成。 [0004] Growth factors EGF can promote epidermal cell growth, skin repair, FGF can promote fibroblast growth, collagen production acceleration. KGF能促进角质细胞分泌纤维蛋白,维持细胞弹性,几种因子的复配能从内而外的促进皮肤结构的改善,加速皮肤修复速度,减少疤痕产生。 KGF can promote the secretion of keratinocyte fibrin, maintenance of cell elasticity, promote the improvement of the skin structure from the inside of the complex from several factors, accelerate skin repair speed and reduce scarring.

[0005] 小分子胜肽在生物的生长、发育和代谢中起着重要作用。 [0005] small molecule peptides play an important role in the growth, development and metabolism of organisms. 人体内的各种生理进程几乎都是由特定的氨基酸序列组成的多肽或蛋白质调控的。 Variety of physiological processes in the human body is almost always by the specific amino acid sequence of the polypeptide or protein regulation. 在皮肤自然老化和护理过程中,胜肽起着重要的作用,如细胞增殖、细胞迀移、炎症、血管发生、色素形成以及蛋白合成及调控。 Natural aging and skin care process, the peptide plays an important role, such as cell proliferation, cell Gan shift, inflammation, angiogenesis, and pigmentation protein synthesis and regulation. 其中棕榈酰信号肽类能促进基质蛋白(matrix protein)尤其是胶原蛋白的合成, 同时还可能增加弹性蛋白、透明质酸、糖胺聚糖和纤维连接蛋白的生成。 Wherein palmitoyl signal peptide can promote collagen synthesis in particular matrix protein (matrix protein), but also may increase elastin, hyaluronic acid, glycosaminoglycans and generating fibronectin. 铜肽能促使神经细胞、免疫相关细胞的生长、分裂和分化,能有效促进伤口愈合。 Copper peptides can induce nerve cells, immune-related cell growth, division and differentiation, can effectively promote the wound healing.

[0006] 不同的作用受体使生长因子和小分子胜肽复配后产生的协同作用进一步促进了皮肤新陈代谢,祛斑除皱,再加上化妆品中其余成分的共同作用,防止皮肤老化,修复受损肌肤,全面改善皮肤状况,复配效果显著优于单独使用。 [0006] The effect of different growth factor receptors, and small molecules that the peptide complex produce a synergistic effect to further promote the metabolism of the skin, wrinkle cream, plus the remaining ingredients in cosmetics cooperate to prevent skin aging, repair the damaged skin, improve overall skin condition, compounding the effect of significantly better than alone.

[0007]目前,关于这方面的研究报道也很多,公布号CNl 02406556A的发明专利公开了一种有助于保持表皮生长因子生物活性的化妆品或护肤品制剂,该制剂主要成分是表皮生长因子、水解胶原蛋白、透明质酸钠、丝肽粉、棕榈酰五肽和肌肽、基质和水,通过多组分保护作用来达到化妆品配方中保持表皮因子生物活性的作用。 [0007] Currently, research reports in this regard are many, the invention Publication No. CNl 02406556A discloses a cosmetic or skin care products helps to maintain the formulation of the epidermal growth factor biological activity, the main component of the formulation epidermal growth factor, hydrolyzed collagen, hyaluronic acid, silk peptide powders, and palmitoyl pentapeptide carnosine, substrate and water, by the protective action is achieved holding the multicomponent biological activity of the epidermal factor effect cosmetic formulations. 公布号为US20140228291A1的发明专利公开了一种护肤凝胶及其使用方法,该凝胶由环状二甲基聚硅氧烷、EGF、TGFfo、FGF 和棕榈酰寡肽等物质制备而成。 Patent Publication No. US20140228291A1 invention discloses a skin gel and method of use, the gel by a cyclic dimethyl polysiloxane, prepared EGF, TGFfo, FGF and palmitoyl oligopeptide from other substances. 但是由于使用的各个物质不同,含量不一致,产品在皮肤上所起的效果也不同。 However, due to various different substance use, inconsistent content, product played on the skin effect is different.

发明内容 SUMMARY

[0008] 本发明的目的是提供一种富含生长因子的化妆品或护肤品制剂及其制备方法。 [0008] The object of the present invention to provide a cosmetic or skin care preparations and a process for preparing enriched in growth factors.

[0009] 为了实现此目的,本发明采用了以下的技术方案: [0009] To achieve this object, the present invention adopts the following technical solution:

[0010] —种富含生长因子的化妆品或者护肤品制剂,其特征在于:主要成分为细胞生长因子,小分子胜肽,水、油脂、表面活性剂、保湿剂、抗氧化剂、防腐剂。 [0010] - growth factor-rich species cosmetic or skin care preparations, characterized in that: the main component of cell growth factor, a small molecule peptide, water, oils, surfactants, humectants, antioxidants, preservatives.

[0011] 作为进一步的改进,所述细胞生长因子占制剂总质量0.0001〜0.001 %,所述小分子胜肽占制剂总质量的0.0001〜0.002 %。 [0011] As a further refinement, the cell growth factor accounting for the total mass of the formulation 0.0001~0.001%, accounting for the small molecule peptide 0.0001~0.002% of the total mass of the formulation.

[0012] 作为进一步的改进,所述的生长因子包括角质细胞生长因子、酸性成纤维细胞生长因子、碱性成纤一种富含生长因子的化妆品或者护肤品制剂,其特征在于:维细胞生长因子、血小板衍生因子和表皮生长因子中的一种或几种组合物。 [0012] As a further refinement, the growth factors including keratinocyte growth factor, acidic fibroblast growth factor, basic fibroblast A cosmetic or skin care preparations rich in growth factors, which is characterized in that: dimensional cell growth factor, platelet derived growth factor and epidermal factor of one or more compositions.

[0013] 作为进一步的改进,所述生长因子均来源于基因工程技术通过基因工程菌发酵纯化所得。 [0013] As a further refinement, the growth factors were derived from genetic engineering techniques by genetic engineering resulting purified fermentation.

[0014] 作为进一步的改进,所述小分子胜肽包括棕榈酰三肽-1、棕榈酰五肽-3、棕榈酰三肽-5、棕榈酰六肽-6、棕榈酰六肽-9、乙酰四肽-5、铜肽、类蛇毒多肽中的一种或几种。 [0014] As a further refinement, the small molecule includes a peptide Palmitoyl Tripeptide-1, Palmitoyl Pentapeptide -3, palmitoyl tripeptide -5, -6 Palmitoyl Hexapeptide, Palmitoyl Hexapeptide-9, -5-acetyl tetrapeptide, copper peptides, snake venom polypeptide one or several.

[0015] 作为进一步的改进,所述的油脂为橄榄油、氢化蓖麻油、辛酸/癸酸甘油三酯、棉籽油、大豆油、沙棘油、霍霍巴油、角鲨烷、羊毛醇、鲸蜡硬脂醇、聚二甲基硅氧烷、聚甲基苯基娃氧烧、环状聚娃氧烧中的一种或多种,其用量为; [0015] As a further refinement, the oil is olive oil, hydrogenated castor oil, caprylic / capric triglyceride, cottonseed oil, soybean oil, sea buckthorn oil, jojoba oil, squalane, lanolin alcohol, cetyl stearyl alcohol, polydimethyl siloxane, polymethyl phenyl burning oxygen baby, baby cyclic polyethylene oxide burning or more in an amount of;

[0016] 所述表面活性剂为吐温20、吐温80、月桂酸钠、硬脂醇硫酸钠、聚氧乙烯脂肪酸酯、 鲸蜡硬脂醇醚-2、鲸蜡硬脂醇醚-21、鲸蜡醇磷酸酯中的一种或几种,其用量为 [0016] The surface active agent is Tween 20, Tween 80, sodium laurate, sodium stearyl alcohol, polyoxyethylene fatty acid esters, ceteareth -2, ceteareth - 21, one or more of cetyl phosphate in an amount of

[0017] 所述的保湿剂为吡咯烷酮羧酸钠、硫酸软骨素、丙三醇、丙二醇、透明质酸钠、三甲基甘氨酸、甲基甘氨酸脯氨酸、木糖醇、脱水木糖醇、木糖醇葡萄糖苷中的一种或几种,其用量为 Humectant [0017] is the sodium pyrrolidone carboxylate, chondroitin sulfate, glycerol, propylene glycol, sodium hyaluronate, trimethyl glycine, glycine-proline methyl, xylitol, xylitol dehydration, one or more glucoside xylitol in an amount of

[0018]所述的制剂还包含pH调节剂,所述pH调节剂为柠檬酸-柠檬酸钠、乳酸-乳酸钠中的任意一种,其用量为:0.1 %-3 %; The preparation of [0018] further comprises a pH adjusting agent, a pH adjusting agent is citric acid - sodium citrate, lactic acid - sodium lactate in any one of an amount of: 0.1% to 3%;

[0019] 所述的抗氧化剂为维生素C、维生素E、核黄素类、烟酸类、金属硫蛋白、超氧化物歧化酶和二甲基甘氨酸中的一种或多种,其用量为 [0019] The antioxidants vitamin C, vitamin E, riboflavin class, niacin, metallothionein, superoxide dismutase and dimethylglycine one or more, an amount of

[0020] 所述的防腐剂为尼泊金甲酯、尼泊金乙酯、尼泊金丙酯、二羟甲基二甲基乙内酰脲、双(轻甲基)咪唑烷基脲、碘代丙炔基丁基氨基甲酸酯和碘丙炔醇丁基氨甲酸酯中的一种或多种,其用量为:0.05%-0.2%; Preservatives [0020] of the methyl paraben, ethyl paraben, propyl paraben, dimethylol dimethyl hydantoin, bis (light meth) imidazolidinyl urea, iodopropynyl butylcarbamate and iodopropynyl butylcarbamate, one or more, in an amount of: 0.05% to 0.2%;

[0021] 所述的化妆品或护肤品制剂形态为霜剂、乳剂、精华水、凝胶剂以及面膜中的任意一种。 [0021] The cosmetic or skin care preparation form of a cream, emulsion, essence of water, gelling agent and any one of the mask.

[0022] 本发明除了上述必要制剂成分外,还可以通过复配其它天然植物提取物以及活性成分达到提升修复作用的目的。 [0022] The present invention in addition to the above essential components of the formulation, but also through complex as well as other natural plant active ingredient to achieve the object of the repairing effect to enhance extraction. 例如,复配有选自甘草提取物、白头翁提取物、秦椒提取物、 木槿花提取物、葡萄籽提取物和菊苣根提取物等中的一种或多种的复配成分。 For example, a compound selected from licorice extract, Pulsatilla extract, pepper extract Qin, extracting one of Althea, grape seed extract, chicory root extract, and the like, or more complex components. 其中所述复配成分占所述化妆品或护肤品总质量的〇. 〇1 %〜1 〇%,优选〇. 1 %〜5 %。 Wherein said compound component comprises square. 〇1% ~ 1% of the square of the total mass of the cosmetic or skin care products, preferably square. 1% ~ 5%.

[0023] 本发明还提供了上述化妆品或者护肤品组合的制备方法,包括以下步骤: [0023] The present invention also provides the above method for preparing a cosmetic or skin care composition, comprising the steps of:

[0024] 将油脂和表面活性剂加热到80°C后搅拌5min溶解均匀,静置冷却至40°C; After [0024] The oil and surfactant is heated to 80 ° C to dissolve uniformly stirred 5min, standing to cool to 40 ° C;

[0025] (1)将油脂和表面活性剂加热到80°C后搅拌5min溶解均匀,静置冷却至40°C ; [0025] (1) The oil and surfactant is heated to 80 ° C 5min stirring homogeneously dissolved, standing to cool to 40 ° C;

[0026] (2)将细胞生长因子和小分子胜肽和抗氧化剂和/或天然植物提取物用去离子水溶解备用,所述去离子水用量为:能使细胞生长因子和小分子胜肽和抗氧化剂和/或天然植物提取物刚好溶解的用量; [0026] (2) the growth factor and peptides and small molecule antioxidants and / or natural plant extracts deionized water was dissolved standby, the deionized water in an amount of: causing the cell growth factors, and small molecules peptide and an antioxidant and / or a natural plant extract is dissolved in an amount just;

[0027] (3)将步骤⑵中的物质加入⑴中的物质中,搅拌3min使其均匀; [0027] (3) the substance added in step ⑵ ⑴ substance stirred homogeneously 3min;

[0028] (4)将保湿剂、pH调节剂、防腐剂组分中的一种或者几种混合溶解并加入步骤(3) 中的混合溶液搅拌均匀即得。 [0028] (4) The one humectant, pH adjusting agent, a preservative, or several components are mixed and dissolved and added to a mixed solution of step (3) was stirred uniformly to obtain.

[0029] 本发明相对于现有技术,具有以下优点: [0029] with respect to the prior art the present invention has the following advantages:

[0030] 本发明活性成分中,生长因子和小分子胜肽的组合能够促进成纤维细胞的生长发育,不断以新的细胞取代老化的细胞,因此通过皮肤的更新产生防皱、祛皱作用;更新衰老细胞,从而降低皮肤中黑色素和有色细胞的含量,减轻皮肤色素沉着。 [0030] The active ingredient of the present invention, the combination of growth factors, and small molecules to peptides capable of promoting the growth of fibroblasts, constantly new cells to replace the aging cell, thus creating wrinkle-free, skin wrinkle by the action of updating; update senescent cells, thereby reducing the content of melanin in the skin and pigmented cells, reduce skin pigmentation. 此外,通过改善微循环来加速色素的代谢,从而达到淡化色素和红润肌肤效果;刺激皮肤肉芽组织的形成和促进肉芽组织的上皮化,还可调节胶原降解及更新,从而起到缩短创伤愈合时间以及减少疤痕形成的作用。 Further, the metabolism is accelerated through the microcirculation of the dye, so as to achieve the effect of reducing pigment and ruddy skin; stimulate the formation of granulation tissue and the skin to promote epithelialization of granulation tissue, collagen degradation can also be adjusted and updated to play a shortened wound healing time and reducing scar formation.

[0031] 所述的保湿剂是纯天然结构物质,吸收快、活性高且具有高度生物兼容性、极易溶于水、非常耐热、耐酸和耐碱、且适用范围广、安全稳定。 [0031] The humectant is a natural structure of the material, rapid absorption, high activity and highly biocompatible, easily soluble in water, very heat, acid and alkali, and a wide range of applications, security and stability. 除此之外还具有柔肤、抗皱、增白、 清爽的功效。 In addition also has a softening, anti-wrinkle, whitening, refreshing effect. 应用在个人护理产品中,能迅速渗透进入皮肤组织,改善皮肤的水分保持力, 激发细胞的活力,赋予肌肤光泽和清爽滋润的效果。 Applications in personal care products, can quickly penetrate into the skin tissue, improve skin moisture retention, stimulate the vitality of cells, giving the skin shiny and refreshing moisturizing effect.

[0032]抗氧化剂中的金属硫蛋白(MT)具有超强的自由基清除能力,其清除· OH自由基的能力是GSH-Px (谷胱甘肽过氧化物酶)的100倍,是SOD (超氧化物歧化酶)的1000倍。 [0032] antioxidants metallothionein (MT) having a superior free radical scavenging capacity, · OH radical scavenging ability is GSH-Px (glutathione peroxidase) 100 times, SOD was (superoxide dismutase) 1000 times. 通过阻断自由基与体内的不饱和脂肪酸的过氧化反应,降低皮肤中过氧化脂质的含量,减少黑色素、脂褐素和蜡样质的生成,作为生物活化剂应用于化妆品中,可起到抗衰老、抗辐射、预防和减轻色斑等多种功效。 By blocking the free radicals and peroxidation of unsaturated fatty acids in the body, reducing the content of skin lipid peroxidation, reducing melanin, lipofuscin and ceroid generation, as biologically active agents used in cosmetics, may serve to anti-aging, anti-radiation, prevention and mitigation of pigmentation and other effects.

[0033] 本发明所添加的天然植物提取物协同生物活性成分的皮肤修复作用,同时又具备了美白祛皱、镇静抗敏的多重作用,大大增加了制剂的适用范围及顺应性。 [0033] The present invention is of added natural plant extracts synergistic skin repair biologically active ingredients, but also have whitening wrinkle removal, allergy sedation multiple roles, greatly increasing the scope of application and conformance of the formulation.

[0034] 该化妆品制剂的配方间相互搭配能加速皮肤细胞的新陈代谢,修复断裂的浅表皮层纤维细胞,对皮肤创面有突出的快速修复功能。 Between formulation [0034] The cosmetic preparations with each other can accelerate the metabolism of skin cells, skin fibroblasts superficial repair broken, projecting quick repair of skin wounds. 应用于皮肤组织的各种损伤,去除暗疮、 痤疮、去痣后的小缺损,换肤后的脱皮、发红、果酸等导致的皮肤灼伤,磨削后的表皮损伤修复等具有突出的效果。 Applied to the skin tissue of a variety of injuries, remove acne, acne, small defects after removal of moles, peeling the skin, redness, skin burns caused by acid and so on, after grinding repair skin damage with outstanding results .

具体实施方式 Detailed ways

[0035] 下面列举一部分具体实施例对本发明进行说明,有必要在此指出的是以下具体实施例只用于对本发明作进一步的说明,不代表对本发明保护范围的限制。 [0035] The following partial list of specific embodiments of the present invention will be described, it is necessary to point out the following specific examples are only for the present invention will be further described, it does not mean to limit the scope of the present invention. 其他人根据本发明做出的一些非本质的修改和调整仍属于本发明的保护范围。 Others still fall within the scope of the invention, according to some non-essential modifications and adaptations of the present invention is made.

[0036] 实施例1皮肤修复乳液 [0036] Example 1 Emulsion skin repair

[0037] 组成乳液的产品的组分及百分数含量为: [0037] The composition of the emulsion composition and percentage content of the product was:

Figure CN104856892BD00061

[0040] 制备方法: [0040] Preparation:

[0041] (1)将A组加热到80°C后搅拌5min溶解均匀,静置冷却至40°C。 [0041] (1) Group A was heated to 80 ° C 5min stirring homogeneously dissolved, standing to cool to 40 ° C.

[0042] (2)将C组分用适量去离子水溶解备用,所述去离子水用量为:能使固体物质刚好溶解的用量; [0042] (2) The component C with an appropriate amount of deionized water was dissolved standby, the amount of deionized water: to make the amount of solid material was dissolved immediately;

[0043] (3)将C组分加入A组分中,搅拌3min使其均匀。 [0043] (3) the C component is added to component A, 3min stirred uniformly.

[0044] ⑷将事先混合溶解的B组分加入上述两组分的混合溶液搅拌均匀即得。 [0044] ⑷ beforehand mixing the B components dissolved were added a mixed solution of the above two components uniformly stirred to obtain.

[0045] 实施例2皮肤修复霜 [0045] Example 2 Skin repair cream embodiment

[0046] 组成霜的产品的组分及百分数含量为: [0046] Components and composition percentage content cream product is:

Figure CN104856892BD00071

[0049] 制备方法: [0049] Preparation:

[0050] (1)将A组加热到80°C后搅拌5min溶解均匀,静置冷却至40°C。 [0050] (1) Group A was heated to 80 ° C 5min stirring homogeneously dissolved, standing to cool to 40 ° C.

[0051] (2)将C组分用适量去离子水溶解备用,所述去离子水用量为:能使细胞生长因子和小分子胜肽和/或天然植物提取物刚好溶解的用量; [0051] (2) The component C with an appropriate amount of deionized water was dissolved standby, the deionized water in an amount of: cytokines, growth-peptides and small molecule and / or a natural plant extract is dissolved in an amount just;

[0052] (3)将C组分加入A组分中,搅拌3min使其均匀。 [0052] (3) the C component is added to component A, 3min stirred uniformly.

[0053] ⑷将事先混合溶解的B组分加入上述两组分的混合溶液搅拌均匀即得。 [0053] ⑷ beforehand mixing the B components dissolved were added a mixed solution of the above two components uniformly stirred to obtain.

[0054] 实施例3皮肤修复精华水。 [0054] Example 3 Repair Lotion skin.

[0055] 组成精华水的产品的组分及百分数含量为: [0055] The composition and percentage of water content of the composition of the product as cream:

Figure CN104856892BD00081

[0058] 制备方法: [0058] Preparation:

[0059] (1)将A组加热到80°C后搅拌5min溶解均匀,静置冷却至40°C ; After stirring for uniform dissolution 5min [0059] (1) Group A was heated to 80 ° C, left to cool to 40 ° C;

[0060] (2)将C组分用适量去离子水溶解备用,所述去离子水用量为:能使细胞生长因子和小分子胜肽和/或天然植物提取物刚好溶解的用量; [0060] (2) The component C with an appropriate amount of deionized water was dissolved standby, the deionized water in an amount of: cytokines, growth-peptides and small molecule and / or a natural plant extract is dissolved in an amount just;

[0061] ⑶将C组分加入A组分中,搅拌3min使其均匀; [0061] ⑶ component C is added to component A, 3min uniformly stirred;

[0062] ⑷将事先混合溶解的B组分加入上述两组分的混合溶液搅拌均匀即得。 [0062] ⑷ beforehand mixing the B components dissolved were added a mixed solution of the above two components uniformly stirred to obtain.

[0063] 实施例4皮肤修复乳液。 [0063] Example 4 Emulsion skin repair.

[0064] 组乳液的产品的组分及百分数含量为:: And the percentage content of component [0064] The group of products is the emulsion ::

Figure CN104856892BD00091

[0067] 制备方法: [0067] Preparation:

[0068] (1)将A组加热到80°C后搅拌5min溶解均匀,静置冷却至40°C ; After stirring for uniform dissolution 5min [0068] (1) Group A was heated to 80 ° C, left to cool to 40 ° C;

[0069] (2)将C组分用适量去离子水溶解备用,所述去离子水用量为:能使细胞生长因子和小分子胜肽和/或天然植物提取物刚好溶解的用量;。 [0069] (2) The component C with an appropriate amount of deionized water was dissolved standby, the deionized water in an amount of: cytokines, growth-peptides and small molecule and / or a natural plant extract is dissolved in an amount just;.

[0070] ⑶将C组分加入A组分中,搅拌3min使其均匀; [0070] ⑶ component C is added to component A, 3min uniformly stirred;

[0071] ⑷将事先混合溶解的B组分加入上述两组分的混合溶液搅拌均匀即得。 [0071] ⑷ beforehand mixing the B components dissolved were added a mixed solution of the above two components uniformly stirred to obtain.

[0072] 效果测试: [0072] Test results:

[0073] 1、防腐性测试 [0073] 1, corrosion resistance test

[0074] 参照美国化妆品、盥洗用品和香精协会(CTFA)推荐的防腐体系效能评价方法,以CTFA推荐的经典的为期28天的防腐单次挑战实验,结果显示,实施例1至4都可以通过测试。 [0074] Referring to the United States in cosmetics, toiletries and fragrances Association (CTFA) recommended performance evaluation method preservative system to CTFA recommended classic 28-day anti-corrosion single challenge experiments, the results show that Example 1-4 are available through test.

[0075] 2、皮肤效果实验 [0075] 2, the skin effect experiments

[0076] 将实施例1至4制得的制剂,分别经20-55岁的100名人员连续试用30天,对比分析评价上述各实施例中所制得的制剂对皮肤的效果。 [0076] Formulation Example 1-4 embodiment will be obtained, respectively, by the persons 20-55 years of 100 consecutive 30-day trial, evaluate the effect of each of the comparative analysis of the embodiments described above prepared formulation to the skin. 结果显示,相比于不加细胞生长因子和小分子胜肽,或者目前市场上的具有皮肤修复效果的产品,本实施例的制剂对皮肤组织的各种损伤,去除暗疮、痤疮、去痣后的小缺损,换肤后的脱皮、发红、果酸等导致的皮肤灼伤, 磨削后的表皮损伤都有良好的修复效果,不仅如此,该制剂还具有美白祛皱、镇静抗敏、柔肤、抗皱、增白、清爽、抗衰老、抗辐射、预防和减轻色斑效果。 The results showed that, compared to a cell growth factor without small molecules and peptides, or a product having a skin repairing effect on the market, various formulations of Example injury to the skin tissue of the present embodiment, the removal of acne, acne, back to moles the small defect, after peeling of skin, redness, skin burns caused by acid and so on, after grinding to repair skin damage have good results, not only that, the preparation also has whitening anti-wrinkle, anti-allergy sedative, soft skin, anti-wrinkle, whitening, refreshing, anti-aging, anti-radiation, preventing and mitigating the effects of pigmentation.

Claims (1)

1. 一种富含生长因子的化妆品或者护肤品制剂,其特征在于:主要成分为细胞生长因子,小分子胜肽,水、油脂、表面活性剂、保湿剂、抗氧化剂、防腐剂; 所述细胞生长因子占制剂总质量O. 〇〇〇1〜O. OO1 %,所述小分子胜肽占制剂总质量的0.0001 〜0.002%; 所述的细胞生长因子包括角质细胞生长因子、酸性成纤维细胞生长因子、碱性成纤维细胞生长因子、血小板衍生因子和表皮生长因子中的一种或几种组合物; 所述生长因子来源于基因工程技术通过基因工程菌发酵纯化所得; 所述小分子胜肽包括棕榈酰三肽-1、棕榈酰五肽-3、棕榈酰三肽-5、棕榈酰六肽-6、棕榈酰六肽-9、乙酰四肽-5、铜肽、类蛇毒多肽中的一种或几种; 所述的油脂为橄榄油、氢化蓖麻油、辛酸/癸酸甘油三酯、棉籽油、大豆油、沙棘油、霍霍巴油、角鲨烷、羊毛醇、鲸蜡硬脂醇、聚 A rich growth factor or cosmetic skin care preparations, characterized in that: the main component of cell growth factor, a small molecule peptide, water, oils, surfactants, humectants, antioxidants, preservatives; the cell growth factor on the total mass of the formulation 〇〇〇1~O O. OO1%, accounting for the small molecule peptide ~0.002 0.0001% of the total mass of the formulation; the cell growth factors include keratinocyte growth factor, acidic fibroblast cell growth factors, basic fibroblast growth factor, platelet derived growth factor and epidermal factor of one or several compositions; growth factor derived from the genetic engineering techniques by genetic engineering resulting purified fermentation; the small molecule peptide comprising palmitoyl tripeptide-1, palmitoyl pentapeptide -3, -6, -9 palmitoyl hexapeptide, acetyl tetrapeptide -5, copper peptides, palmitoyl tripeptide venom polypeptide -5, palmitoyl hexapeptide of one or more; the oil is olive oil, hydrogenated castor oil, caprylic / capric triglyceride, cottonseed oil, soybean oil, sea buckthorn oil, jojoba oil, squalane, lanolin alcohol, cetyl stearyl alcohol, polyethylene 甲基硅氧烷、聚甲基苯基硅氧烷、环状聚硅氧烷中的一种或多种,其用量为所述表面活性剂为吐温20、吐温80、月桂酸钠、硬脂醇硫酸钠、聚氧乙烯脂肪酸酯、鲸蜡硬脂醇醚-2、鲸蜡硬脂醇醚-21、鲸蜡醇磷酸酯中的一种或几种,其用量为所述的保湿剂为吡咯烷酮羧酸钠、硫酸软骨素、丙三醇、丙二醇、透明质酸钠、三甲基甘氨酸、甲基甘氨酸脯氨酸、木糖醇、脱水木糖醇、木糖醇葡萄糖苷中的一种或几种,其用量所述的制剂还包含PH调节剂,所述pH调节剂为柠檬酸-柠檬酸钠、乳酸-乳酸钠中的任意一种,其用量为:〇.1%-3%; 所述的抗氧化剂为维生素C、维生素E、核黄素、烟酸、金属硫蛋白、超氧化物歧化酶和二甲基甘氨酸中的一种或多种,其用量为:0.1 %_1 %; 所述的防腐剂为尼泊金甲酯、尼泊金乙酯、尼泊金丙酯、二羟甲基二甲基乙内酰脲、双(轻甲基)咪 Methyl polysiloxane, methylphenyl polysiloxane, cyclic polysiloxanes or more, an amount of the surfactant is Tween 20, Tween 80, sodium laurate, stearyl alcohol sulfate, polyoxyethylene fatty acid esters, ceteareth -2, ceteareth -21, cetyl phosphate, one or several, according to an amount of humectant is sodium pyrrolidone carboxylic acid, chondroitin sulfate, glycerol, propylene glycol, sodium hyaluronate, trimethyl glycine, glycine-proline methyl, xylitol, dehydration xylitol, glucoside one or more, an amount of the formulation further comprises a pH adjusting agent, a pH adjusting agent is citric acid - sodium citrate, lactic acid - sodium lactate in any one of an amount of: 〇.1% - 3%; the antioxidants vitamin C, vitamin E, riboflavin, niacin, metallothionein, superoxide dismutase and dimethylglycine or more, an amount of: 0.1% _1%; said preservative is methyl paraben, ethyl paraben, propyl paraben, dimethylol dimethyl hydantoin, bis (light meth) imidazole 烷基脲、碘代丙炔基丁基氨基甲酸酯和碘丙炔醇丁基氨甲酸酯中的一种或多种,其用量为:〇.〇5%-〇.2%; 所述的化妆品或护肤品制剂形态为霜剂、乳剂、精华水、凝胶剂以及面膜中的任意一种; 所述制剂中还包含天然植物提取物,所述的天然植物提取物为甘草提取物、白头翁提取物、秦椒提取物、木權花提取物、匍萄轩提取物和菊苣根提取物中的一种或多种; 所述天然植物提取物占所述化妆品或护肤品总质量的〇. 1%〜5% ; 所述制剂的制备方法,其包括以下步骤: ⑴将油脂和表面活性剂加热到80°C后搅拌5min溶解均匀,静置冷却至40°C; (2)将细胞生长因子和小分子胜肽和抗氧化剂和天然植物提取物用去离子水溶解备用,所述去离子水用量为:能使细胞生长因子和小分子胜肽和抗氧化剂和天然植物提取物刚好溶解的用量; ⑶将步骤⑵中的物质加入(1)中的 Alkyl urea, iodopropynyl butyl carbamate, and iodopropynyl butylcarbamate, one or more, an amount of: 〇.〇5% -〇.2%; the cosmetic or skin care preparation form of a cream described later, emulsion, essence of water, gelling agent and any one of the mask; the formulation further comprising a natural plant extract, a natural plant extract is an extract of licorice , PCR extracts, Qin pepper extract, flower extract wood right, creeping grape Xuan extracting one thing and chicory root extract or more; the natural plant extracts accounting for the total mass of the cosmetic or skin care products . 1% ~ 5% square; the method of preparing the formulation, comprising the steps of: dissolving uniformly stirred 5min after ⑴ grease and a surfactant was heated to 80 ° C, left to cool to 40 ° C; (2) the cell growth factors and peptide and small molecule antioxidants and natural plant extracts deionized water was dissolved standby, the amount of deionized water: Cytokines and growth-peptide and small molecule antioxidants and natural plant extracts just the amount of dissolved; ⑶ ⑵ the substance added in step (1) 物质中,搅拌3min使其均匀; (4)将保湿剂、pH调节剂、防腐剂组分中的混合溶解后加入步骤(3)中的混合溶液搅拌均匀即得。 Materials, uniformly stirring 3min; (4) After mixing the humectant, pH adjusting agent, preservative components dissolved in a mixed solution added in step (3) was stirred uniformly to obtain.
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