CN105769604B - A kind of painting type water laser accunputure of through skin corium and preparation method thereof - Google Patents
A kind of painting type water laser accunputure of through skin corium and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/733—Alginic acid; Salts thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/75—Anti-irritant
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Abstract
The invention discloses painting type water laser accunputure of a kind of through skin corium and preparation method thereof, the painting type water laser accunputure of the through skin corium includes following weight parts raw material：15 25 parts of sodium hyaluronates and 15 25 parts of collagens, 69 part of 1,3 propylene glycol, 13 parts of L sodium ascorbates, 13 parts of L serines, 15 parts of stabilizers, 15 portions of sun-screening agents, 60 450 parts of water.The present invention can moisturizing it is sun-proof, the instant moisturizing of dry skin, rapid osmotic, deep layer nourishing, long-acting water lock are given respectively, realize three-dimensional moisturizing, increase skin elasticity, keep skin fine and smooth, smooth, glossy, and is tested by antiallergic, it is without any side effects, securely and reliably, it can be used as the long-time service of facial mask class skin-protection product.
Description
Technical field
The present invention relates to technical field of skin care, and in particular to a kind of painting type water laser accunputure of through skin corium and its preparation
Method.
Background technology
The water loss of skin is related with many factors, such as air-conditioning, environmental pollution, Seasonal conversion, and air humidity reduces,
And then there is aging phenomenons and many skin problems such as drying, jaundice, intensely dark pool, relaxation, wrinkle in skin together.
Sodium hyaluronate, also known as hyaluronic acid, are the substances of naturally occurring in tissue, it can draw own wt
1000 times of moisture has the function of surprising water suction, water lock and Water Cycle.With advancing age, sodium hyaluronate can be continuous
Loss.Thus sodium hyaluronate becomes at present the best and most effective natural replenishing water and preserving moisture factor on the market.But due to cost and
The reason of technology, it is still the sodium hyaluronate of macromolecular that many products are most commonly used, contains only the shield of unimodal molecular weight sodium hyaluronate
The effect of skin product is limited, can not play the best skin-care effect of sodium hyaluronate.
With advancing age, the aggravation of environmental pollution endures the young white collar of work weight to the fullest extent because of ultraviolet light, dust, useless
The reasons such as gas, computer radiation cause skin problems occur, along with people are to beautiful continuous pursuit and to health
Demand, with whitening, moisturizing, remove wrinkle, light spot etc. and obtained most consumers for the beauty mask class product of primary efficacy
Favor.Accordingly, we have invented a kind of painting type water laser accunputure of through skin corium, active component can ooze rapidly at normal temperatures and pressures
Skin corium is arrived thoroughly, by applying face or being applied to skin epidermis, you can infiltration epidermis, go directly skin corium；Raw material is simple, at clearly demarcated
Really, obvious effect, simple for process.
Invention content
Aiming at the above shortcomings existing in the prior art, the first technical problem to be solved by the present invention is to provide a kind of straight
Up to the painting type water laser accunputure of skin corium.
The second technical problem to be solved by the present invention is to provide the preparation of the painting type water laser accunputure of above-mentioned through skin corium
Method.
Purpose of the present invention is to what is be achieved through the following technical solutions：
A kind of painting type water laser accunputure (and being properly termed as facial treatment essence liquid) of through skin corium, by following weight parts raw material group
At：15-25 parts of sodium hyaluronates and 15-25 parts of collagens, 6-9 parts of 1,3- propylene glycol, 1-3 parts of L-AA sodium, 1-3 parts L-
Propylhomoserin, 1-5 part stabilizer, 1-5 portions of sun-screening agents, 60-450 parts of water.
The stabilizer is mixed by guar gum, trehalose, xylitol, the guar gum, trehalose, xylitol
Mass ratio is (1-3)：(1-3)：(1-3).
The sun-screening agent is mixed by rue leaf glycosides, polygonin, barbaloin, the rue leaf glycosides, polygonin, aloe
The mass ratio of glycosides is (1-3)：(1-3)：(1-3).
The sodium hyaluronate is sodium hyaluronate of the molecular weight in 300-900 dalton.
The collagen is collagen of the molecular weight in 300-900 dalton.
The present invention also provides the preparation methods of the painting type water laser accunputure of above-mentioned through skin corium, include the following steps：
(1) the water vacuum that temperature is 80-100 DEG C is pumped into emulsification pot, stabilizer homogeneous is added under processing condition
Processing；
(2) when temperature is reduced to 10-40 DEG C, sodium hyaluronate, collagen, 1,3-PD, L- is added into emulsification pot
Sodium ascorbate, Serine are uniformly mixed the painting type water laser accunputure to get the through skin corium.
Specifically, in the present invention：
Collagen, No. CAS：9064-67-9.
Sodium hyaluronate, also known as hyaluronic acid, No. CAS：9004-61-9.
L-AA sodium, No. CAS：134-03-2.
Serine, No. CAS：56-45-1.
Rue leaf glycosides, No. CAS：153-18-4, structural formula are as follows：
Polygonin, No. CAS：27208-80-6, structural formula are as follows：
Barbaloin, No. CAS：1415-73-2, structural formula are as follows：
Guar gum, No. CAS：9000-30-0.
Trehalose, No. CAS：99-20-7, structural formula are as follows：
Xylitol, No. CAS：16277-71-7.
1,3-PD, No. CAS：504-63-2
The present invention can moisturizing it is sun-proof, give respectively the instant moisturizing of dry skin, rapid osmotic, deep layer nourish, long-acting water lock,
It realizes three-dimensional moisturizing, increases skin elasticity, keep skin fine and smooth, smooth, glossy, and tested by antiallergic, without any pair
Effect securely and reliably can be used as the long-time service of facial mask class skin-protection product.
Specific implementation mode
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention
Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents be changed to the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, according to the present invention
Technical spirit any simple modification that following embodiment is made or equivalent variations, all fall in protection scope of the present invention.
Raw material introduction in embodiment：
Collagen, No. CAS：9064-67-9, molecular weight is in 500-700 dalton.
Sodium hyaluronate, No. CAS：9004-61-9, molecular weight is in 500-700 dalton.
Embodiment 1
Weigh each raw material (parts by weight)：20 parts of sodium hyaluronates, 20 parts of collagens, 7 parts of 1,3- propylene glycol, 2 parts of L- Vitamin Cs
Sour sodium, 1.5 parts of Serines, 3 parts of stabilizers, 3 portions of sun-screening agents, 90 parts of water.
The stabilizer is uniformly mixed to obtain by guar gum, trehalose, xylitol, the guar gum, trehalose,
The mass ratio of xylitol is 1：1：1.
The sun-screening agent is uniformly mixed to obtain by rue leaf glycosides, polygonin, barbaloin, the rue leaf glycosides, tiger
Cane glycosides, barbaloin mass ratio be 1：1：1.
The present invention also provides the preparation methods of the painting type water laser accunputure of above-mentioned through skin corium, include the following steps：
(1) water vacuum at a temperature of 90 °C is pumped into emulsification pot, 2000 revs/min of stabilizer is added under processing condition
Homogenization 5min；
(2) when temperature is reduced to 35 DEG C, sodium hyaluronate, 2000 revs/min of homogenization 5min are added into emulsification pot；It is added
Collagen, 2000 revs/min of homogenization 5min；1,3-PD, 2000 revs/min of homogenization 5min are added；It is eventually adding
L-AA sodium, Serine, 2000 revs/min of homogenization 5min；It is eventually adding sun-screening agent, 2000 revs/min of homogenizations
5min；Room temperature is down to get the painting type water laser accunputure of the through skin corium of embodiment 1.
Embodiment 2
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Stabilizer be uniformly mixed to obtain by guar gum, trehalose, the guar gum, trehalose mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 2.
Embodiment 3
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Stabilizer be uniformly mixed to obtain by guar gum, xylitol, the guar gum, xylitol mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 3.
Embodiment 4
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Stabilizer be uniformly mixed to obtain by trehalose, xylitol, the trehalose, xylitol mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 4.
Embodiment 5
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Sun-screening agent be uniformly mixed to obtain by rue leaf glycosides, polygonin, the rue leaf glycosides, polygonin mass ratio be 1：1.I.e.
Obtain the painting type water laser accunputure of the through skin corium of embodiment 5.
Embodiment 6
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Sun-screening agent be uniformly mixed to obtain by rue leaf glycosides, barbaloin, the rue leaf glycosides, barbaloin mass ratio be 1：1.I.e.
Obtain the painting type water laser accunputure of the through skin corium of embodiment 6.
Embodiment 7
The painting type water laser accunputure that the skin corium that goes directly is prepared by the raw material proportioning and method of embodiment 1, differs only in：It is described
Sun-screening agent be uniformly mixed to obtain by polygonin, barbaloin, the polygonin, barbaloin mass ratio be 1：1.Up to reality
Apply the painting type water laser accunputure of the through skin corium of example 7.
Test case 1
Anti-aging test is carried out to the painting type water laser accunputure for the skin corium that goes directly made from embodiment 1-7, test index is skin
Skin elasticity and keratoderma moisture.
Skin elasticity year skin aging and reduce, therefore skin elasticity is the one of the important signs that for judging skin aging, is
The effect of anti-aging cosmetics, detects essential project.
Experimental method：Per group selection health, skin it is normal, without the tested crowd of cosmetic allergic contact dermatitis history, age 20-45 Sui,
Middle male 20, women 20.One week is as a treatment course, totally four courses for the treatment of, every time using preceding by facial cleansing, and each face cleaning
Time is identical, uses corresponding product, each group to use corresponding product daily 1 time after skin dries, 10g is taken uniformly to be applied to face
On.
It measures：It selects Face and cheek as test zone, is tested after product 10min to be used.It is surveyed using skin elasticity
It tries instrument and measures skin elasticity curve, according to the amount of tension of skin and resilience data, moisturizing that can be to cosmetics and anti-aging work(
It can be assessed.
Experimental result：Skin elasticity test is to be based on suction and stretch principle, and generating one on the surface of tested skin bears
Pressure, by one special test probe of skin inspiration, skin is inhaled into depth and is surveyed by a non-contact optical detection system
, measurement result usually indicates that unit 1, numerical value is bigger with an index, illustrates that skin elasticity is stronger.The present invention is through
Influence of the painting type water laser accunputure of skin corium to skin elasticity is as shown in table 1.
Table 1：Skin elasticity test value table
| Group | 0 week | 1 week | 2 weeks | 3 weeks | 4 weeks |
| Embodiment 1 | 0.479 | 0.557 | 0.659 | 0.696 | 0.739 |
| Embodiment 2 | 0.478 | 0.538 | 0.631 | 0.662 | 0.713 |
| Embodiment 3 | 0.480 | 0.531 | 0.627 | 0.659 | 0.702 |
| Embodiment 4 | 0.481 | 0.532 | 0.635 | 0.672 | 0.715 |
| Embodiment 5 | 0.479 | 0.535 | 0.633 | 0.676 | 0.713 |
| Embodiment 6 | 0.482 | 0.529 | 0.641 | 0.680 | 0.721 |
| Embodiment 7 | 0.501 | 0.539 | 0.636 | 0.679 | 0.709 |
Note：After carry out statistical analysis, the results showed that P ＜ 0.05, difference have statistical significance.
As it can be seen from table 1 the present invention goes directly, skin elasticity, explanation can be remarkably reinforced in the painting type water laser accunputure of skin corium
The painting type water laser accunputure of the through skin corium of the present invention has good anti-aging and the skin and other effects that compacts, and enhances elasticity of skin,
Deep layer repairs skin, makes skin dynamic.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer is using guar gum, trehalose, xylitol compounding) skin
Skin elasticity is substantially better than embodiment 2-4 (stabilizer is using guar gum, trehalose, the arbitrary the two compounding of xylitol)；Compare implementation
Example 5 and embodiment 6-8, embodiment 5 (sun-screening agent is using rue leaf glycosides, polygonin, barbaloin compounding) skin elasticity are substantially better than
Embodiment 6-8 (sun-screening agent is using rue leaf glycosides, polygonin, the arbitrary the two compounding of barbaloin).
Moisture is important one of the moulding substance of skin epidermis cuticula, and horny layer of epidermis is thinning when skin aging, cutin
Natural moisture preserving component content is reduced in layer, and skin hydration ability declines, and moisture of skin, which is lost, to be increased；Meanwhile cell shrinkage tissue
There is histological structure and morphological change and skin is made to gradually appear fine wrinkles in atrophy.
It can reflect the degree and Moisturizer of skin aging to a certain extent by measurement to moisture of skin
Effect.
Experimental method：Per group selection healthy skin, no tested crowd of cosmetic allergic contact dermatitis history, age 20-45 Sui, wherein male
20, women 20.25 DEG C ± 1 DEG C of room temperature；Humidity 50% ± 5%.Face is cleaned when experiment, and everyone face cleaning time is identical,
It selects Face and cheek 4cm × 4cm skins as test zone after skin dries, 0.8g is taken uniformly to be applied on the face.
It measures：10min is tested after Face and cheek (4cm × 4cm) test zone, product to be used.
(1) with digital skin moisture detector come the water content of aspect of measure skin of cheek.Using 1h after corresponding product,
After 2h, 4h, 6h, Face and cheek moisture content of skin is surveyed, measurement is averaged three times.
(2) with percutaneous moisture loss measuring instrument come the water loss amount of aspect of measure skin of cheek.Use corresponding product
Afterwards after 1h, 2h, 4h, 6h, Face and cheek moisture of skin windage is surveyed, measurement is averaged three times.
Experimental result：By testing water content of stratum corneum is weighed using the variation of keratoderma capacitance before and after product
Variation to evaluate the moisture-keeping efficacy of cosmetics.The method carries out quantification to skin keratin moisture, can be sensitive
Ground reacts the variation of skin moisture content, and favorable reproducibility, is one of current common method of moisture-keeping cosmetics efficacy assessments.This
Influence of the product to skin moisture content is as shown in table 2.
Table 2：Moisture test value list position：%
Note：After carry out statistical analysis, the results showed that P ＜ 0.05, difference have statistical significance
As can be seen from Table 5, the painting type water laser accunputure moisturizing effect of the through skin corium of the present invention is good, moisture preserving time is long, says
The painting type water laser accunputure of the bright through skin corium of the present invention can effectively inhibit excess oil, balance skin grease, while oil-control
Also there is moisturizing effect, lasting moisturizing, softening skin.
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (stabilizer is using guar gum, trehalose, xylitol compounding) water
Point content is apparently higher than embodiment 2-4 (stabilizer is using both the arbitrary compounding of guar gum, trehalose, xylitol)；Compare implementation
Example 1 and embodiment 5-7, embodiment 1 (sun-screening agent is using rue leaf glycosides, polygonin, barbaloin compounding) moisture are apparently higher than
Embodiment 5-7 (sun-screening agent is using rue leaf glycosides, polygonin, the arbitrary the two compounding of barbaloin).
Test case 2
Radiation protection test is carried out to the painting type water laser accunputure for the skin corium that goes directly made from embodiment 1 and embodiment 5-7.
Test method is with reference to patent application 201410613299.8 specification embodiment 2 of page 5, and [0033] section is extremely
[0038] section.
The cell used is to immortalize epithelial cell (Hacat cells), purchased from Beijing consonance cell resource center.Cultivate item
Part is DMEM culture mediums (Gibco, the U.S.), antibacterium and fungi containing 10% FBS (Hyclone, Australia) and 1%
Antibiotic (Invitrogen, the U.S.), in 37 DEG C, CO2Culture in a concentration of 5% incubator.
Cell set 1 group of blank control group, 1 group of negative control group and with 4 groups of test groups, blank control group is not added through true
The painting type water laser accunputure of cortex is also handled without UVB (ultraviolet light), and through corium is not added on the culture medium of negative control group
The painting type water laser accunputure of layer, 4 groups of test groups add the painting type water laser accunputure 0.1mg of the through skin corium of embodiment 5-8 respectively.
Hacat cells are with 4 × 104The holes cells/ are inoculated in 12 orifice plates, cell inoculation for 24 hours after, cell sucks culture medium,
It is primary that PBS cleaning cells are added, removes PBS, is put into progress UVB processing in ultraviolet irradiation case.UVB irradiates source wavelength 280-
320nm, wave crest 312nm.It is 20mJ/cm that UVB, which handles Hacat cell irradiation doses,2。
IL-1 alpha content user IL-1 α/IL-1F1DuoSet of the Hacat cells after UVB is handled in culture supernatant
ELISA kit (R＆D System, the U.S.) is detected, and IL-6 content detections are tried using humanIL-6DuoSet ELISA
Agent box (R＆D System, the U.S.) carries out, and TNF-a content detections use human TNF-aDuoSet ELISA kits (R＆D
System, the U.S.) it carries out, the same specification of method.
The content of content difference divided by negative control group that each group experiment is measured, is multiplied by 100 parts, obtains relative amount.
Result of calculation see the table below 3.
Table 3：The painting type water laser accunputure of through skin corium carries out radiation protection test result table
| IL-1 α (part) | IL-6 (part) | TNF-a (part) | |
| Blank control group | 11.4 | 18.1 | 14.4 |
| Negative control group | 100 | 100 | 100 |
| Embodiment 1 | 48.9 | 63.1 | 58.0 |
| Embodiment 5 | 49.8 | 66.1 | 61.3 |
| Embodiment 6 | 51.3 | 65.9 | 60.9 |
| Embodiment 7 | 51.2 | 65.5 | 60.4 |
After UVR processing can cause Hacat cells, cell anticusp inflammation factor IL-1 α, IL-6 and TNF-a can be caused to express and increased
Add, in culture medium after painting type water laser accunputure of the addition using the through skin corium of 1-7 of the embodiment of the present invention, significantly reduces IL-1
The content of α, IL-6 and TNF-a, it can be seen that, the inflammatory reaction caused by UVB can be inhibited, protection is risen to epithelial cell and is made
With.
Comparing embodiment 1 and embodiment 5-7, embodiment 1 (sun-screening agent is using rue leaf glycosides, polygonin, barbaloin compounding)
To system the inflammatory reaction caused by UVB it is with obvious effects better than embodiment 5-7 (sun-screening agent using rue leaf glycosides, polygonin, aloe
The arbitrary the two compounding of glycosides).
Claims (4)
1. a kind of painting type water laser accunputure of through skin corium, which is characterized in that be made of following weight parts raw material：15-25 parts of glass
Uric acid and 15-25 parts of collagens, 6-9 parts of 1,3- propylene glycol, 1-3 parts of L-AA sodium, 1-3 parts of Serines, 1-5 parts it is steady
Determine agent, 1-5 portions of sun-screening agents, 60-450 parts of water；
The stabilizer is mixed by guar gum, trehalose, xylitol, the guar gum, trehalose, xylitol quality
Than for (1-3)：(1-3)：(1-3)；
The sun-screening agent is mixed by rue leaf glycosides, polygonin, barbaloin, the rue leaf glycosides, polygonin, barbaloin
Mass ratio is (1-3)：(1-3)：(1-3).
2. the painting type water laser accunputure of through skin corium as described in claim 1, which is characterized in that the sodium hyaluronate is molecule
Measure the sodium hyaluronate in 300-900 dalton.
3. the painting type water laser accunputure of through skin corium as described in claim 1, which is characterized in that the collagen is point
Collagen of the son amount in 300-900 dalton.
4. the preparation method of the painting type water laser accunputure of through skin corium as described in any one of claims 1-3, which is characterized in that
Include the following steps：
(1) the water vacuum that temperature is 80-100 DEG C is pumped into emulsification pot, stabilizer homogenization is added under processing condition；
(2) when temperature is reduced to 10-40 DEG C, into emulsification pot, addition sodium hyaluronate, collagen, 1,3-PD, L- are anti-bad
Hematic acid sodium, Serine are uniformly mixed the painting type water laser accunputure to get the through skin corium.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610141193.1A CN105769604B (en) | 2016-03-11 | 2016-03-11 | A kind of painting type water laser accunputure of through skin corium and preparation method thereof |
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| US10456343B2 (en) | 2017-01-26 | 2019-10-29 | L'oreal | Microemulsion compositions comprising polydatin and method of use |
| CN111529442A (en) * | 2020-05-08 | 2020-08-14 | 紫水晶(山西)再生医学科技有限公司 | Water light injection for medical and beauty and preparation method thereof |
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| CN103932909A (en) * | 2014-04-25 | 2014-07-23 | 北京博辉瑞进生物科技有限公司 | Composition for facial care and preparation method of composition |
| CN105342867A (en) * | 2015-12-15 | 2016-02-24 | 上海相宜本草化妆品股份有限公司 | Herbal composition submicron lipid carrier and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103932909A (en) * | 2014-04-25 | 2014-07-23 | 北京博辉瑞进生物科技有限公司 | Composition for facial care and preparation method of composition |
| CN105342867A (en) * | 2015-12-15 | 2016-02-24 | 上海相宜本草化妆品股份有限公司 | Herbal composition submicron lipid carrier and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 中药及天然植物防晒作用研究进展;张婷婷;《中华中医药学刊》;20140331;第556-558页 * |
| 天然植物防晒作用的筛选研究;郭媛媛;《天津中医药》;20080228;第69-70页 * |
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