KR102101920B1 - Cosmetic composition for improving skin condition comprising human epidermal growth factor, peptides and functional materials - Google Patents

Abstract

Disclosed herein is a cosmetic composition for improving skin condition comprising human epithelial cell growth factor, peptide and functional material having excellent skin condition improving effect. The cell growth factor-containing composition with improved stability, which is an aspect of the present invention, contains a high concentration of glycerin and contains a combination of a cellulose derivative and a hyaluronic acid salt, thereby stably containing various types of cell growth factors and peptides. In addition to cell growth factors such as can stably contain a variety of biologically derived functional substances. In addition, the composition is not sticky when applied to the skin, and has excellent skin absorbing power, and thus has an excellent feeling of use and improvement in skin condition.

Description

Cosmetic composition for improving skin condition containing human epithelial cell growth factor, peptide and functional material {COSMETIC COMPOSITION FOR IMPROVING SKIN CONDITION COMPRISING HUMAN EPIDERMAL GROWTH FACTOR, PEPTIDES AND FUNCTIONAL MATERIALS}

Disclosed herein is a cosmetic composition for improving skin condition comprising human epithelial cell growth factor, peptide and functional material having excellent skin condition improving effect.

Recently, with the development of life science technology, interest in the therapeutic effect of bio-factors existing in vivo has increased. In particular, some cell growth factors are known to have excellent skin regeneration effects, and growth factors such as human epithelial cell growth factor and fibroblast growth factor are listed in the International Cosmetic Ingredients (ICID) of the American Cosmetics Association (CTFA). In Korea, the Ministry of Food and Drug Safety has been approved as a cosmetic raw material and is also used as a cosmetic raw material at home and abroad, so it is mass produced through various genetic engineering techniques. However, the cell growth factor, there is a problem that the natural biological activity is rapidly lost due to easy degeneration due to minute environmental changes such as temperature, pressure, acidity (pH) and ion concentration.

For this reason, attempts to secure thermal stability of cell growth factors (Korean Patent Publication 10-2017-0127704) are continuing, and the patent relates to an EGF heat stabilizing composition, which is applied when applied to the human body as a cosmetic composition There was a problem that many users feel uncomfortable due to stickiness. In addition, Korean Patent 10-0748390B1 relates to a quasi-release sustained-release film formulation for treating wounds containing EGF, which contains L-histidine and an antioxidant as a heat stabilizer, and glycerin as a plasticizer, so that these formulations cannot be used as a cosmetic. There is this.

KR 10-2017-0127704 A KR 10-0748390 B1

In one aspect, an object of the present invention is to provide a cosmetic composition that can stably contain various types of cell growth factors.

In one aspect, an object of the present invention is to provide a cosmetic composition that can stably contain various types of substances such as functional peptides derived from living organisms.

In one aspect, the object of the present invention is to provide a cosmetic composition excellent in use even when cell growth factors and bio-derived functional substances (peptides, etc.) are contained in various concentrations.

In one aspect, an object of the present invention is to improve the skin absorption rate of the cell growth factor and the bio-derived functional substance (peptide, etc.) contained in the cosmetic composition.

In one aspect, the object of the present invention is to provide a composition excellent in skin condition improving effect.

In one aspect, the present invention provides a cell growth factor; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And it includes a hyaluronic acid salt, the stability of each component is improved, and provides a cosmetic composition excellent in improving the skin condition with high skin absorption.

The cell growth factor-containing composition with improved stability, which is an aspect of the present invention, contains a high concentration of glycerin and contains a combination of a cellulose derivative and a hyaluronic acid salt, thereby stably containing various types of cell growth factors and peptides. Various biologically-derived functional substances such as biologically and physicochemically may be stably contained. In addition, the composition is not sticky when applied to the skin, so it has an excellent feeling of use and excellent skin absorption, so it has an excellent skin condition improving effect.

1 is a cosmetic composition containing glycerin at different concentrations (15, 30, 45 wt%), which is one aspect of the present invention, stored at 30 ° C. for 30 days, followed by HPLC using cell growth factors, peptides and whitening functionality As a result of analyzing the stability of the material, it is a result showing the stability (%) of epithelial cell growth factor after 15 days and 30 days.
2 is a result of analyzing an improvement rate of skin wrinkle amount (FIG. 2A) and a maximum depth improvement of skin wrinkle (FIG. 2B) before, after 2 weeks, and after 4 weeks of use of the composition, which is an aspect of the present invention.
FIG. 3 is a result photograph (antera 3D) on the effect of improving skin wrinkles showing changes in skin condition before and after 2 and 4 weeks of use of the composition, which is one aspect of the present invention.
Figure 4 is a result of analyzing the skin brightness improvement rate before and after the use of the composition, 2 weeks, 4 weeks after use of one aspect of the present invention.

Hereinafter, the present invention will be described in detail.

In one aspect, the invention provides cell growth factors; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And hyaluronic acid salts.

In addition, the present invention, cell growth factor; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And hyaluronic acid salts.

In the present specification, the stability may include biological activity and physicochemical stability, and the improvement in stability improves the phenomenon that the active ingredient contained in the composition is precipitated, separated, and deteriorated by external environmental factors such as temperature. Can mean

Despite the excellent skin improvement effect, epithelial cell growth factor and the like have been pointed out as a problem that it is unstable at room temperature and thus has poor utilization as a cosmetic composition.

On the other hand, the composition of the various cell growth factors, as well as at low temperature (about 0 ~ 5 ℃), at room temperature (20 ~ 30 ℃) and high temperature (30 ℃) does not occur biological and physicochemical degeneration such as cell growth factors Stable

Preferably, the cell growth factor included in the composition includes epidermal growth factor (EGF), cosmetic ingredient name: S-oligopeptide-1); And fibroblast growth factor (FGF), cosmetic ingredient name: S-polypeptide-1. In the above aspect, the composition, by including epithelial cell growth factor and fibroblast growth factor among cell growth factors, to obtain a better skin condition improvement effect, such as wrinkle improvement, whitening, moisturizing, or skin tone improvement effect Can be.

In one aspect, the composition, including a combination of glycerin, a cellulose derivative and a hyaluronic acid salt, can improve the stability of various active ingredients contained in the composition and the user's sensitivity.

In addition, in the above aspect, the cellulose derivative may include one or more selected from the group consisting of ethyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, and hydroxyethyl cellulose, preferably hydroxyethyl cellulose. It may include. By including the cellulose derivative, the composition can improve the use sensibility by controlling the viscosity of the cosmetic compared to when only glycerin is included.

The vitamin may include vitamin B or a derivative thereof, for example, niacinamide. Niacinamide may be included in an amount of 0.5 to 5% by weight, preferably 2 to 5% by weight, based on the total weight of the composition.

In addition, the nucleotide of the composition may include adenosine, and the adenosine may be included in an amount of 0.01 to 0.1% by weight, preferably 0.01 to 0.06% by weight.

In addition, the composition may include sodium hyaluronate as a hyaluronic acid salt. By including sodium hyaluronate, the composition can significantly improve user sensitivity compared to when only glycerin is included.

In one aspect, the cell growth factor may include human cell growth factor. For example, the cell growth factor may include human epithelial cell growth factor, or human fibroblast growth factor.

In addition, in the above aspect, the fibroblast growth factor may include acidic or basic fibroblast growth factor.

The glycerin, based on the total weight of the composition, may be included in 30% by weight or more and 60% by weight or less. The glycerin, preferably 30 to 50% by weight, more preferably 40 to 50% by weight, most preferably, may be included in 43 to 48% by weight.

In addition, the composition may include the cell growth factor in an amount of 0.1 to 100 ppm (w / w) based on the total weight of the composition.

Specifically, the cell growth factor may preferably include 0.1 to 30 ppm, more preferably 0.2 to 10 ppm (w / w).

In one embodiment, each of the epithelial cell growth factor and fibroblast growth factor may be included in 6 ~ 14ppm (w / w), preferably 8 ~ 12ppm (w / w). Within the above content range, the epithelial cell growth factor and fibroblast growth factor may be included in a weight ratio of 0.8 to 1.2: 0.8 to 1.2, and most preferably, in a ratio of about 1: 1.

In addition, the cellulose derivative may be included in an amount of 0.05% by weight or more and less than 0.2% by weight based on the total weight of the composition, and preferably, 0.05 to 0.15% by weight, and more preferably 0.08 to 0.12% by weight.

On the other hand, in the present specification, when the numerical value is represented by the range "X to Y", it is interpreted as X or more and Y or less unless otherwise specified.

In addition, the hyaluronic acid salt, based on the total weight of the composition, may be included in 0.05% by weight or more and less than 0.2% by weight, preferably, 0.05 to 0.15% by weight, more preferably 0.08 to 0.12% by weight have.

In addition, the composition may further include, in addition to the above components, a functional material, in particular, a complex derived from a bio-derived functional material or a bio-derived functional material (a material synthesized using a bio-derived functional material). The bio-derived functional material may include peptides, amino acids, and the like. The peptide may include, but is not limited to, acetylhexapeptide-8, palmitoylpentapeptide-4, copper tripeptide-1, and the like.

The peptide, based on the total weight of the composition, may be included in an amount of 0.0005% by weight or more to ~ 0.4% by weight or less, and specifically, the composition is a peptide, acetylhexapeptide-8, copper tripeptide-1, and palmitoylpenta Peptide-4, or one or more of them.

When the composition includes all of acetylhexapeptide-8, coppertripeptide-1, and palmitoylpentapeptide-4, each of acetylhexapeptide-8, coppertree peptide-1, and palmitoylpentapeptide-4 is a composition Based on the total weight, 0.05 to 0.15% by weight, preferably, may be included in 0.08 to 0.12% by weight. In addition, the composition may further include amino acids, specifically, arginine, glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine, tryptophan, and the like. The composition is preferably an amino acid, may further include an arginine. The arginine, based on the total weight of the composition, may include 0.01 to 0.1% by weight, preferably, 0.01 to 0.07% by weight, more preferably 0.03 to 0.05% by weight.

The functional material may further include taurine, and the taurine may be included in an amount of 0.1 to 3% by weight, preferably 0.5 to 1.5% by weight.

In addition, the composition, in order to improve the stability of the components contained in the composition, may further include an acidic component, for example, may further include a citric acid. The citric acid may be included in an amount of 0.005 to 0.02% (w / v) based on the total weight of the composition, and more preferably, in an amount of 0.008 to 0.015%.

In addition, the composition, for wrinkle improvement; For skin elasticity enhancement; For skin whitening; For pore reduction; For improving skin problems; Or a skin moisturizing composition.

In addition, the composition may include a cosmetic composition.

The wrinkle improvement may include improving individual wrinkle depths as well as the amount of wrinkles.

The cosmetic composition may be formulated as a cream, essence, gel, makeup base, lotion, skin, or cosmetic liquid.

The cosmetic composition, for a variety of skin types (dry, moderate to dry, neutral, oily, oily) can be used for oily skin types.

Hereinafter, the present invention will be described in more detail through the following examples. However, the following examples are provided for the purpose of illustration only to aid understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Production Example] Preparation of cosmetic composition

A cosmetic composition was prepared at the contents shown in Table 1 below. Specifically, glycerin, sodium hyaluronate, hydroxyethyl cellulose, adenosine, and niacinamide were dissolved and mixed in 70 ° C purified water, cooled to 30 ° C, and the remaining raw materials were added and mixed to mix A, B, Table 1, C was prepared.

Raw material name A B C Purified water Remaining amount (to 100%) Remaining amount (to 100%) Remaining amount (to 100%) glycerin 45% 45% 45% Niacinamide 2% 2% 2% 1,2-hexanediol 2% 2% 2% Taurine One% One% One% Caprylyl glycol 0.5% 0.5% 0.5% Sodium hyaluronate 0.2% 0.1% - Hydroxyethyl cellulose - 0.1% 0.2% Acetyl hexapeptide-8 0.1% 0.1% 0.1% Copper tripeptide-1 0.1% 0.1% 0.1% Palmitoylpentapeptide-4 0.1% 0.1% 0.1% Arginine 0.04% 0.04% 0.04% Adenosine 0.04% 0.04% 0.04% Citric Acid 0.01% 0.01% 0.01% S-oligopeptide-1 0.001% 0.001% 0.001% S-polypeptide-1 0.001% 0.001% 0.001%

[Example 1] Usability evaluation

[Example 1-1] Selection of candidate compositions

The sensitivity of use to the cosmetic compositions A, B, and C prepared in Example 1 was evaluated for 10 women. The subjects were 3 in their 30s, 3 in their 40s, and 4 in their 50s, and the sensibility of use, absorbency, and the degree of stickiness and moistness after use were first investigated.

For each question, 5 points for "very yes (very satisfied)", 4 points for "yes (satisfied)", 3 points for "normal (average)", and 2 points for "not (unsatisfied)" , The average value divided by the number of subjects who summed up the scores of "not at all (very dissatisfied)" by 1 point is shown in Table 2.

division A B C Feeling when first applied 3.4 4.2 4.7 absorptivity 4.2 4.6 2.8 Sticky after use 3.1 4.8 4.9 Moist after use 4.1 4.4 3.0

As a result, a composition B having a better feel and absorbency than other compositions having a composition B containing 0.1% by weight of sodium hyaluronate and hydroxyethylcellulose, respectively, was selected as a candidate composition.

[Example 1-2] Stability evaluation of functional ingredients in cosmetic composition according to glycerin concentration

Peptides, whitening functional materials and growth factors (epithelial cell growth factor and fibroblasts) in cosmetic compositions with different concentrations of glycerin (15.30, 45% by weight) in consideration of the characteristics of the cosmetic composition stored and used at room temperature Stability of growth factor) was analyzed by high performance liquid chromatography (HPLC) using an XBridge C18 column from Water.

Each cosmetic composition is stored at 30 ° C and extracted with functional components at 15-day intervals, followed by distilled water solution containing 0.1% trifluoroacetic acid and acetonitrile using acetonitrile at a rate of 1 mL / min. Reels were analyzed with a linear concentration gradient from 0% to 70%. By analyzing the cosmetic composition to which different concentrations of glycerin (15.30, 45% by weight) were added by HPLC, the area for the peaks of the functional ingredients after 15 and 30 days was measured to the peak of the functional ingredients on the 0th day (first manufacturing date) Table 3 shows the results calculated as a percentage by dividing the area by area.

Glycerin 15% by weight Glycerin 30% by weight Glycerin 45% by weight Elapsed time 15 days later 30 days later 15 days later 30 days later 15 days later 30 days later Niacinamide 98.03 98.06 98.01 97.97 98.38 98.37 Copper tripeptide-1 97.92 96.22 98.01 97.98 99.16 99.06 Adenosine 98.39 97.73 98.90 98.87 99.36 99.33 Acetyl hexapeptide-8 98.95 97.18 97.92 97.93 99.95 99.93 Epithelial cell growth factor 69.09 53.40 79.67 62.14 98.85 97.45 Fibroblast growth factor 96.58 96.81 97.90 96.69 99.53 99.60 Palmitoylpeptide peptide-4 95.31 94.98 96.72 96.69 99.73 99.99

 As a result, at 30 ° C., epithelial cell growth factor is more stable at about 30% after 15 days and at least about 45% after 30 days in cosmetic compositions containing high concentrations of glycerin than compositions containing low concentrations of glycerin. Maintained.

In addition, other components (fibroblast growth factors, peptides, adenosine, niacinamide, etc.) also, when the content of glycerin exceeds 40% by weight, it was found that the stability is further improved.

[Example 1-3] Emotional evaluation of composition B

A questionnaire evaluation of subjective use sensibility was performed on 22 women who were subjects of the human skin clinical trial for the cosmetic composition B selected from Example 1-1.

The evaluation subjects were 8 in their 30s, 9 in their 40s, and 5 in their 50s. After using the cosmetic composition B twice a day for 4 weeks, the applied cosmetic composition B had a spreadability, absorbency, stickiness, fragrance, and overall feeling of use. Was investigated. For each question, 5 points for “very satisfied (very satisfied)”, 4 points for “yes (satisfied)”, 3 points for “normal (average)”, 2 points for “not (unsatisfied)”, The scores of “not at all (very dissatisfied)” were scored on a question-by-item basis, and the average value divided by the number of subjects was shown in Table 4 for each age and skin type.

division Spreadability absorptivity Stickiness incense Overall usability All (22) Average 4.32 4.23 3.73 3.09 4.23 Deviation 0.72 0.92 0.94 1.02 0.87 age 30s
(8 people) Average 4.25 4.00 3.25 3.00 4.00 Deviation 0.71 0.76 0.89 0.53 0.76 40s
(9 people) Average 4.33 4.33 3.89 2.67 4.11 Deviation 0.87 1.12 0.93 1.32 1.05 50 cars
(5 people) Average 4.40 4.40 4.22 4.00 4.80 Deviation 0.55 0.89 0.89 0.00 0.45 Skin type inattention
(5 people) Average 4.40 4.40 3.20 2.80 4.00 Deviation 0.55 0.89 1.10 0.45 1.00 Medium dryness (6 people) Average 4.00 4.00 3.67 3.33 3.83 Deviation 0.89 0.89 1.03 0.52 1.17 neutrality
(7 people) Average 4.26 4.29 4.14 2.71 4.43 Deviation 0.76 1.11 0.69 1.38 0.53 Jisung
(3 people) Average 4.67 4.67 4.00 3.67 4.67 Deviation 0.58 1.00 1.00 1.15 0.58 Intelligence
(1 person) Average 5.00 5.00 4.00 4.00 5.00 Deviation 0.00 0.00 0.00 0.00 0.00

As a result, in the questions about the spreadability, absorbency, and overall usability, satisfaction tended to be higher with age, especially in the skin type of middle-aged. The overall use sensitivity for the cosmetic composition B was 4.0 or higher in all age groups, particularly 4.80, which was the highest in the 50s.

[Example 2] Evaluation of composition B effect for improving skin condition

After using the cosmetic composition B twice a day for 4 weeks, it improves the skin moisturizing power before and after use, improves the texture of the skin, improves the brightness of the skin, reduces pores, improves the elasticity of the skin, improves wrinkles, regenerates the skin, and purchases motivation. Was investigated. For each question, 5 points for “very satisfied (very satisfied)”, 4 points for “yes (satisfied)”, 3 points for “normal (average)”, 2 points for “not (unsatisfied)”, The scores of “not at all (very dissatisfied)” were scored on a question-by-item basis, and the average values and deviations divided by the number of subjects were shown in Table 5 by age and skin type.

[Table 5]

As a result, it was confirmed that the evaluator using the cosmetic composition B had an effect of improving the skin condition evenly across all age groups, and evaluated that there was a willingness to purchase and continuously use it in all age groups and all skin types.

[Example 3] Confirmation of wrinkle improvement effect of composition B

From one week before the start of the test, the use of therapeutic agents, cosmetics and quasi-drugs aimed at improving the skin, which can affect the evaluation results, is prohibited, and medical treatment and massage treatment are also prohibited. For evaluation, the cosmetic composition B was provided to the test subjects, and after washing in the morning and evening, a certain amount was spread on the face and then knocked by hand (2 times / day / person). After the morning cleansing, the cosmetic composition B is completely absorbed into the skin, and then an additional product is used. After the evening cleansing, only the cosmetic composition B is used without using any products such as skin and lotion. To check the effect of cosmetic composition B on improving skin wrinkles, use the equipment (skin 3D meter, Antera 3D) before (1st), 2 weeks (2nd), and 4 weeks (3rd) of cosmetic composition B use. Table 6 shows the results of analyzing the amount and depth of wrinkles in the skin. The significance of the liver before and after the use of the cosmetic composition B was confirmed using the SPSS (Ver. 19) program ( P <0.05, paired t-test), and the wrinkle improvement rate (%) compared to before use was calculated by substituting the following formula 2 is shown.

[Equation 1]

Improvement rate (%) = (measured value after use-measured value before use) / measured value before use X 100 (%)

Before use After two weeks 4 weeks later Wrinkle amount (mm) Average 12.11 11.44 11.26 ^* Standard Deviation 2.79 2.03 2.06 Maximum depth of wrinkle (mm) Average 0.14 0.13 0.13 Standard Deviation 0.04 0.04 0.04

^* P <0.05, number of subjects: 22

As a result, in the subjects using composition B, the wrinkle improvement rate of 5.54% after use for 2 weeks and 7.02% after use for 4 weeks was shown as compared with before use. After 4 weeks of use, the amount of wrinkles decreased to a statistically significant level (P <0.05, paired t-test). In the results of the measurement of the maximum wrinkle value, the maximum depth improvement rate of wrinkles was 7.14% after 2 and 4 weeks of use compared to that in the test subject using the cosmetic composition B.

[Example 4] Confirm the skin whitening effect of composition B

Preparation of the test subject before the experiment and the use of the cosmetic composition B were performed in the same manner as in Example 5. In order to confirm the effect of improving the skin brightness of the cosmetic composition B, the subject (Spectrophotometer) was used before (1st), after 2 weeks (2nd), and after 4 weeks (3rd) in the subjects using the cosmetic composition B. By measuring the change in skin brightness is shown in Table 7. The significance of the liver before and after the use of the cosmetic composition B was confirmed using the SPSS (Ver. 19) program (P <0.05), and the skin brightness improvement rate (%) compared to before use was calculated using the same calculation formula as in Example 3 ( Fig. 4).

Before use After two weeks 4 weeks later Skin brightness (L) Average 63.01 63.13 63.55 ^* Deviation 2.46 2.36 2.45

^* P <0.05, number of subjects: 22

As a result, when the composition of the present invention was used, it was found that after 4 weeks of use compared to before use, it showed a statistically significant improvement in skin brightness of 0.86% (P <0.05, paired t-test).

Claims (13)

Cell growth factor;
Peptides;
Nucleotides;
vitamin;
glycerin;
Cellulose derivatives; And
Contains hyaluronic acid salt,
The glycerin and cellulose derivatives, based on the total weight of the composition, each containing 43 to 48% by weight and 0.08 to 0.12% by weight, a cosmetic composition containing improved cell growth factor stability. Cell growth factor;
Peptides;
Nucleotides;
vitamin;
glycerin;
Cellulose derivatives; And
Contains hyaluronic acid salt,
The glycerin and cellulose derivatives, based on the total weight of the composition, each containing 43 to 48% by weight and 0.08 to 0.12% by weight, a skin growth factor-containing cosmetic composition with improved skin absorption. The method according to claim 1 or 2,
The cell growth factor,
Epidermal growth factor (EGF); And
A composition comprising fibroblast growth factor (FGF). The method according to claim 1 or 2,
The peptide,
Acetyl hexapeptide-8, copper tripeptide-1, and palmitoyl pentapeptide-4 comprising at least one selected from the group consisting of,
The nucleotide includes adenosine,
The vitamin, a composition comprising niacinamide. The method according to claim 1 or 2,
The cellulose derivative,
A composition comprising at least one selected from the group consisting of ethylcellulose, hydroxypropylmethylcellulose, methylcellulose, and hydroxyethylcellulose. The method according to claim 1 or 2,
The hyaluronic acid salt, a composition comprising sodium hyaluronate. The method according to claim 1 or 2,
The cell growth factor,
A composition comprising human cell growth factors. delete The method according to claim 1 or 2,
The composition, based on the total weight of the composition,
The cell growth factor, including 0.1ppm or more and 20ppm (w / w) or less,
The composition comprising the hyaluronic acid salt in an amount of 0.05% by weight or more and less than 0.2% by weight. The method according to claim 1 or 2,
The peptide, based on the total weight of the composition, contained in 0.0005 to 0.4% by weight, composition. The method according to claim 1 or 2,
The composition,
Wrinkle improvement; For skin elasticity enhancement; For skin whitening; For pore reduction; For improving skin problems; Or for skin moisturizing. According to claim 3,
The fibroblast growth factor, an acidic fibroblast growth factor or a basic fibroblast growth factor, a composition. delete

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