KR102279755B1 - Cosmetic composition, and Fabrication method thereof - Google Patents

Abstract

Provided are a cosmetic composition and a method for preparing the same, which comprise: a solid phase first formulation including sodium hyaluronate, a first nucleic acid, a first amino acid, a first transforming growth factor- β2 (TGF-β2) analogue, a first peptide, and a vitamin; and a liquid phase second formulation including a second nucleic acid, a second amino acid, a second transforming growth factor and a second peptide, in which the sodium hyaluronate included in the first formulation contains a low molecular weight first sodium hyaluronate, a medium molecular weight second sodium hyaluronate, and a high molecular weight third sodium hyaluronate, in which a content of the third high molecular weight sodium hyaluronate is 15 to 25 wt% based on the total weight of the sodium hyaluronate. The present study was conducted as a part of Gyeonggi-do's 2020 beauty industry promotion support project titled Beauty R&D and clinical trial support.

Description

Cosmetic composition and its manufacturing method {Cosmetic composition, and Fabrication method thereof}

It relates to a cosmetic composition and a method for preparing the same.

Due to air pollution, environmental pollution and virus spread, the occurrence of a number of various skin troubles is increasing due to wearing a mask for a long time and living indoors. Accordingly, interest in skin beauty and skin care that can soothe these skin troubles and deliver active ingredients to the skin is growing. Since the beauty consumer trend is also complex, there is a trend to demand effective products that contain trendy ingredients without harmful ingredients.

When the composition of the composition is complicated while including an expensive active ingredient, it is difficult to maintain the continuous effect of the active ingredient in general formulations such as skin, lotion, essence, and cream. Therefore, there is an increasing trend in research to maintain the lasting effect of the active ingredient.

There is a need for a cosmetic composition that meets these needs and a method for simply manufacturing the same.

Korean Patent Publication No. 2019-0130245

One aspect is to provide a cosmetic composition that can be stored for a long time and provides improved skin elasticity.

Another aspect is to provide a method for preparing a cosmetic composition prepared by the above method.

According to one aspect,

a first solid phase preparation comprising sodium hyaluronate, a first nucleic acid, a first amino acid, a first TGF-β2 (Tranforming Growth Factor-β2) analogue, a first peptide, and a vitamin; and

A liquid phase second formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide;

The sodium hyaluronate salt included in the first formulation includes a low molecular weight first sodium hyaluronate salt, a medium molecular weight second sodium hyaluronate salt, and a high molecular weight third sodium hyaluronate salt,

The content of the high molecular weight third sodium hyaluronate salt is 15 to 25 wt% based on the total weight of the sodium hyaluronate salt, a cosmetic composition is provided.

According to the other side,

Provided is a solid phase first formulation comprising sodium hyaluronate, a first nucleic acid, a first amino acid, a first TGF-β2, Tranforming Growth Factor-β2 analog, a first peptide, and a vitamin to do;

providing a liquid phase second formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide; and

Comprising the step of preparing a cosmetic composition by combining the first agent and the second agent,

The sodium hyaluronate salt included in the first formulation includes a low molecular weight first sodium hyaluronate salt, a medium molecular weight second sodium hyaluronate salt, and a high molecular weight third sodium hyaluronate salt,

The content of the high molecular weight third sodium hyaluronate salt is 15 to 25 wt% based on the total weight of the sodium hyaluronate salt, a cosmetic composition manufacturing method is provided.

According to one aspect, the new cosmetic composition can be stored for a long time and provide improved skin elasticity.

According to another aspect, by the novel cosmetic composition manufacturing method, the above-described cosmetic composition can be prepared simply.

1 is an image of a first formulation solid prepared in Example 1.
2 is an image of the cosmetic composition prepared in Example 1.
3 is an evaluation result of dermal elasticity for the cosmetic compositions prepared in Example 1 and Comparative Example 1.
4 is an evaluation result of cheek area elasticity for the cosmetic compositions prepared in Example 1 and Comparative Example 1.
5 is an evaluation result of skin elasticity restoring force for the cosmetic compositions prepared in Example 1 and Comparative Example 1.

The present inventive concept described below can apply various transformations and can have various embodiments, and specific embodiments are illustrated in the drawings and described in detail. However, this is not intended to limit the present inventive concept to a specific embodiment, and it should be understood to include all transformations, equivalents, or substitutes included in the technical scope of the present inventive concept.

In describing each figure, like reference numerals have been used for like elements. In the accompanying drawings, the dimensions of the structures are enlarged than the actual size for clarity of the present invention. Terms such as first, second, etc. may be used to describe various elements, but the elements should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, a first component may be referred to as a second component, and similarly, a second component may also be referred to as a first component. The singular expression includes the plural expression unless the context clearly dictates otherwise.

In the present application, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It is to be understood that it does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof. Also, when a part of a layer, film, region, plate, etc. is said to be "on" another part, this includes not only the case where it is "directly on" another part, but also the case where another part is in the middle. Conversely, when a part of a layer, film, region, plate, etc. is said to be “under” another part, it includes not only cases where it is “directly under” another part, but also cases where another part is in the middle.

Hereinafter, a cosmetic composition according to exemplary embodiments and a method for preparing the same will be described in more detail.

Cosmetic composition according to an embodiment, sodium hyaluronate (sodium hyaluronate), a first nucleic acid (nucleic acid), a first amino acid (amino acid), a first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) a solid phase first formulation comprising an analog, a first peptide, and a vitamin; and a second liquid phase formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide, wherein the sodium hyaluronate included in the first formulation is a low molecular weight agent. 1 containing sodium hyaluronate, a second sodium hyaluronate salt of medium molecular weight, and a third sodium hyaluronate salt of high molecular weight, wherein the content of the third sodium hyaluronate salt of high molecular weight is 15 to about the total weight of the sodium hyaluronate salt 25wt%. The content of the third high molecular weight sodium hyaluronate salt included in the first formulation may be, for example, 16 to 24 wt%, 17 to 23 wt%, or 18 to 22 wt%, based on the total weight of the sodium hyaluronate salt. The first formulation and the second formulation include the above-mentioned components, and the content of the third high molecular weight hyaluronic acid is 15 to 25 wt% based on the total weight of the sodium hyaluronate salt, thereby providing improved skin absorption and skin elasticity without stickiness and it is possible to simply prepare a cosmetic composition having good moisturizing power. If the content of the high molecular weight third hyaluronic acid is too low, skin absorption may decrease and moisture retention may decrease. When the content of the high molecular weight third hyaluronic acid is too high, the moisturizing power is improved, but the viscosity of the cosmetic composition increases, so that the stickiness may increase and the solubility of the first agent may decrease.

First, the first solid phase preparation includes sodium hyaluronate, a first nucleic acid, a first amino acid, a first TGF-β2 (Tranforming Growth Factor-β2) analog, a first peptide, and a vitamin do.

Further, the liquid phase second formulation comprises a second nucleic acid, a second amino acid, a second transforming growth factor analog and a second peptide. The order in which the first agent and the second agent are provided may be selected according to required conditions. The first agent and the second agent may be, for example, stored separately from each other and then combined at the time of use and applied to the skin in the form of a formulation.

Sodium hyaluronate or hyaluronic acid is the most distributed in the skin in the living body and plays an important role in maintaining the life of the skin. Its production decreases after the age of 25 and causes skin wrinkles. Sodium hyaluronate is a natural moisturizing ingredient that not only moisturizes the skin by binding the surrounding moisture, but also helps protect the skin from the external environment. As the hyaluronic acid content of the skin decreases with age, aging of the skin progresses, and supplying hyaluronic acid to the skin through cosmetics containing hyaluronic acid can provide a moisturizing effect to the skin.

The content of the low molecular weight first sodium hyaluronate included in the first formulation may be, for example, 15 to 45 wt%, 16 to 44 wt%, 17 to 43 wt%, or 18 to 42 wt%, based on the total weight of the sodium hyaluronate salt. . If the content of the low molecular weight first sodium hyaluronate salt is too low, skin absorption may be reduced. When the content of the low molecular weight first sodium hyaluronate salt is too high, the moisturizing power is lowered, and the effect of the cosmetic composition may not last for a long time by being rapidly decomposed in the skin.

The content of the second medium-molecular-weight sodium hyaluronate salt included in the first formulation is, for example, 35 to 65 wt%, 36 to 64 wt%, 37 to 63 wt%, 38 to 62 wt%, 39 to 61 wt%, based on the total weight of the sodium hyaluronate salt. % or 40 to 60 wt%. If the content of the second medium molecular weight sodium hyaluronate salt is too low, uniform dispersion of the low molecular weight first sodium hyaluronate salt and the high molecular weight third sodium hyaluronate salt may not be obtained in the cosmetic composition. When the content of the second medium-molecular-weight sodium hyaluronate salt is too high, the skin absorption power may be reduced or the moisturizing power may be reduced by decreasing the content of the low molecular weight first sodium hyaluronate salt and the high molecular weight third sodium hyaluronate salt.

The content of the first high molecular weight sodium hyaluronate salt included in the first formulation may be, for example, 15 to 25 wt%, 16 to 24 wt%, 17 to 23 wt%, or 18 to 22 wt% based on the total weight of the sodium hyaluronate salt. . If the content of the first high molecular weight sodium hyaluronate salt is too low, skin moisturizing power may be reduced. If the content of the first low molecular weight sodium hyaluronate salt is too high, skin stickiness may increase.

The sodium hyaluronate salt included in the first formulation is, for example, 15 to 45 wt% of the low molecular weight first sodium hyaluronate salt; 35 to 65 wt% of a second sodium hyaluronic acid salt; and 15 to 25 wt% of the third high molecular weight hyaluronate sodium salt. By including the sodium hyaluronate salt having a composition within this range, the first formulation provides improved skin absorption, excellent skin elasticity and good moisturizing power at the same time, and the overall feeling of use can be improved by having improved solubility.

The weight average molecular weight of the first low molecular weight sodium hyaluronate salt contained in the first formulation is 0.15 MDa to less than 0.5 MDa, and the weight average molecular weight of the second medium molecular weight sodium hyaluronate salt is 0.5 MDa to less than 1.3 MDa, and the high molecular weight agent 3 The weight average molecular weight of the sodium hyaluronate salt may be less than 1.3 MDa to 1.8 MDa. By having the molecular weight in this range of the first, second, and third sodium hyaluronate salts, a cosmetic composition that provides more improved moisturizing power and skin elasticity may be provided.

The first nucleic acid and the second nucleic acid included in the first agent and the second agent are each independently one or more selected from DNA sodium salt (Deoxyribonucleic acid, sadium salt), DNA, RNA sodium salt (Ribonucleic acid sadium salt), and RNA. can

The first nucleic acid and the second nucleic acid can be extracted from, for example, salmon wolves. All starting materials used for the extraction and purification of the first and second nucleic acids are, for example, materials of mineral origin and not of animal origin. The first nucleic acid and the second nucleic acid play an important role in the regulation of protein biosynthesis in the metabolism of living cells. Stores genetic information that is passed on to sub-cells through identical copies of DNA molecules during cell differentiation. DNA sodium salt, for example, can promote the activation of cell repair mechanisms, can keep the skin moisturized for a long time, and can absorb body water 10,000 times its own weight. The first nucleic acid and the second nucleic acid can promote collagen and elastic fiber production when combined with amino acids, help prevent skin sensitivity and strengthen skin resistance, help protein synthesis, and promote skin metabolism.

The weight average molecular weight of the first nucleic acid and the second nucleic acid may be independently of each other, for example, 1000 kDa to 3000 kDa, 1500 kDa to 2500 kDa, or 1700 kDa to 2100 kDa. When the first nucleic acid and the second nucleic acid have a weight average molecular weight within this range, skin elasticity may be further improved. The content of the first nucleic acid included in the first agent is greater than 0 to 10 wt%, greater than 0 to 5 wt%, greater than 0 to 1 wt%, greater than 0 to 0.5 wt%, or greater than 0 to 0.1 wt%, based on the total weight of the first agent % can be The content of the second nucleic acid included in the second agent is more than 0 to 10 wt%, more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, based on the total weight excluding distilled water included in the second agent; or greater than 0 to 0.1 wt%.

The first amino acid and the second amino acid included in the first agent and the second agent may be, independently of each other, one or more selected from, for example, leucine, glycine, proline, and lysine.

The first amino acid and the second amino acid are essential components that are not synthesized in the human body, and have an effect of protecting the skin barrier. In addition, the first amino acid and the second amino acid serve as a component of protein to regulate the pH and moisture balance of the skin, and may help to remove dead skin cells and improve rough skin texture.

The content of the first amino acid included in the first agent is greater than 0 to 10 wt%, greater than 0 to 5 wt%, greater than 0 to 1 wt%, greater than 0 to 0.5 wt%, or greater than 0 to 0.1 wt%, based on the total weight of the first agent can be The content of the second amino acid included in the second agent is more than 0 to 10 wt%, more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, based on the total weight excluding distilled water included in the second agent; or greater than 0 to 0.1 wt%.

The first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) analog and the second transforming growth factor analog included in the first agent and the second agent are, independently of each other, Acetyl SH-hexapeptide-8 sp (Acetyl sh -Hexapeptide-8 SP), Acetyl H-oligopeptide-29 SP (Acetyl sh-Oligopeptide-29 SP), and Acetyl H- oligopeptide-30 SP (Acetyl sh-Oligopeptide-30 SP) may be at least one selected from the group consisting of.

Transforming growth factor (TGF), which is an origin factor of neural stem cells that regulates signal transduction between cells, is a substance that induces transformation of fibroblasts, for example, in the process of cell development, cell differentiation and proliferation. , migration, survival, extracellular matrix production, and the immune system. Transforming growth factors include, for example, TGF-β1, TGF-β2, and TGF-β3. Transforming growth factor-beta2 (TGF-β2), for example, is a secreted protein known as a cytokine that performs most cellular functions and can perform various functions in the human body, Helps promote the cell cycle. Transforming growth factor-beta2 (TGF-β2) has excellent whitening effect by inhibiting melanin production through the activity of Wnt signaling pathway inhibitors in melanocytes. In addition, it promotes hair growth through dermal papilla cell activation and hair follicle stem cell differentiation by balancing the transforming growth factor-beta2 (TGF-β2) and BMP 4/6 signaling pathways, and TIMP- secreted from neural stem cells 1 and TIMP-2 proteins inhibit the MMP family, which is involved in aging and wrinkles, and thus have an effect on aging and wrinkle improvement. Transforming growth factor-beta2 (TGF-β2) exists in various forms and functions to regulate various cellular activities, which is usually related to the mechanism of action of the membrane and is a major regulatory component in wound healing due to its soluble and insoluble forms. By screening a suitable protein and cutting the screened protein in the form of an Oligo Peptide of 500 Da or less that is easy to penetrate into the skin, the stability is increased to increase the stability of the first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) analogue and the second conversion Growth factor analogs were prepared. Therefore, the effect provided by the above-described transforming growth factor-beta2 (TGF-β2) can be expected by using the TGF-β2 (Tranforming Growth Factor-β2) analogue and the second transforming growth factor analogue.

The content of the first transforming growth factor analog included in the first agent is greater than 0 to 5 wt%, greater than 0 to 1 wt%, greater than 0 to 0.5 wt%, greater than 0 to 0.1 wt%, or greater than 0 based on the total weight of the first agent. to 0.05 wt%. The content of the second growth-converting factor analog included in the second agent is more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, more than 0 to the total weight excluding distilled water included in the second agent. 0.1 wt%, or greater than 0 to 0.05 wt%.

The first peptide and the second peptide included in the first agent and the second agent independently of each other are sh-oligopeptide-5, copper tripeptide-1, acetyl tetrapeptide -11 (Acetyl Tetrapeptide-11), Palmitoyl Tripeptide-1, Palmitoyl Pentapeptide-4, Tripeptide-1 (Tripeptide-1), Acetylhexapeptide-8 (Acetyl Hexapeptide-8), Hexapeptide-9, Palmitoyl Tetrapeptide-7, Nonapeptide-1, and RH-oligopeptide-1 (rh It may be at least one selected from -Oligopeptide-1).

The first peptide and the second peptide may, for example, protect the skin and impart a conditioning effect to the skin, thereby helping to maintain healthy and energized skin. In addition, the first peptide and the second peptide, for example, can be easily absorbed into the skin to keep the skin moist, thereby providing elasticity and energy to the skin tired due to aging, thereby restoring vitality.

The content of the first peptide included in the first agent is more than 0 to 10 wt%, more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, more than 0 to 0.1 wt%, based on the total weight of the first agent; greater than zero to 0.05 wt%, greater than zero to 0.01 wt%, greater than zero to 0.005 wt%, greater than zero to 0.001 wt%, or greater than zero to 0.0001 wt%. The content of the second peptide included in the second agent is more than 0 to 10 wt%, more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, based on the total weight excluding distilled water included in the second agent; greater than 0 to 0.1 wt%, greater than 0 to 0.05 wt%, greater than 0 to 0.01 wt%, greater than 0 to 0.005 wt%, greater than 0 to 0.001 wt%, or greater than 0 to 0.0001 wt%.

Vitamins included in the first formulation, for example, retinol (vitamin A), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinic acid (vitamin B3), carnitine (carnitine, vitamin B4), Pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), folic acid (vitamin B9), cobalamine (vitamin B12), ascorbic acid (vitamin C) ), phylloquinone (vitamin K), menaquinone (menaquinone, vitamin K), and menadione (menadione, vitamin K) may be at least one selected from the group consisting of.

Vitamins play an important role in caring for aging and dry skin exposed to UV rays and cold by supplying moisture to activate the epidermal layer.

The vitamin content included in the first formulation is more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, more than 0 to 0.1 wt%, more than 0 to 0.05 wt%, 0 based on the total weight of the first formulation greater than 0.01 wt%, greater than zero to 0.005 wt%, or greater than zero to 0.001 wt%.

The first formulation may further include, for example, one or more selected from vitamin derivatives, sugar derivatives, amino acid derivatives, preservatives, and moisturizing agents, in addition to the above-described components. The first agent is, for example, magnesium ascorbyl phosphate as a vitamin derivative, mannitol as a sugar derivative, acetyl cystein as an amino acid derivative, and 1,2-hexanediol (1,2) as a preservative. -hexandiol), and butylene glycol as a moisturizer, but not necessarily limited thereto, and used as vitamin derivatives, sugar derivatives, amino acid derivatives, preservatives, and moisturizers in the art. Anything is possible

The content of vitamin derivatives, sugar derivatives, amino acid derivatives, preservatives, and moisturizing agents included in the first formulation is independently of each other more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 based on the total weight of the first formulation to 0.5 wt%, greater than 0 to 0.1 wt%, or greater than 0 to 0.05 wt%.

The second formulation may further include one or more active ingredients selected from plant extracts, ferment extracts, fermentation filtrates, and honey extracts in addition to the above-mentioned ingredients.

The second agent is, for example, Galactomyces Ferment Filtrate as a fermentation filtrate, Bacillus-Soybean Ferment Extract as a ferment extract, Centella Asiatica Extract as a plant extract ), Horseshoe Mushroom Extract (Fomes Officinalis Mushroom Extract), Portulaca Oleracea Extract, Bambusa Arundinacea Stem Extract, but not necessarily limited thereto, but plant extracts, As long as it is used as a ferment extract or ferment filtrate, it is possible.

The content of plant extracts, ferment extracts, fermentation filtrates, honey extracts, etc. included in the second agent is independently from each other more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 based on the total weight of the second agent to 0.5 wt%, greater than 0 to 0.1 wt%, or greater than 0 to 0.05 wt%.

The second formulation may further include one or more additives selected from, for example, a moisturizer, a surfactant, an antioxidant and a preservative.

The types of moisturizers, surfactants, antioxidants, and preservatives included in the first formulation are not particularly limited, and any type used in the art may be used.

The content of the moisturizing agent, surfactant, antioxidant, and preservative included in the second formulation is independently of each other more than 0 to 5 wt%, more than 0 to 1 wt%, more than 0 to 0.5 wt%, more than 0 based on the total weight of the second formulation to 0.1 wt%, or greater than 0 to 0.05 wt%.

A solid first formulation comprising a sodium hyaluronate, a first nucleic acid, a first amino acid, a first TGF-β2 (Tranforming Growth Factor-β2) analogue, a first peptide, and a vitamin is, for example, hyaluronic acid. It may be a freeze-dried product of a first mixed aqueous solution comprising sodium ronate, a first nucleic acid, a first amino acid, a first TGF-β2, Tranforming Growth Factor-β2 analog, a first peptide, and a vitamin.

The first mixed aqueous solution is, for example, a first aqueous solution containing a low molecular weight first sodium hyaluronate salt, a second aqueous solution containing the middle molecular weight second sodium hyaluronate salt, and a third high molecular weight sodium hyaluronate salt A third aqueous solution comprising, a fourth aqueous solution comprising the medium molecular weight second sodium salt of hyaluronic acid and a first nucleic acid, a fifth solution comprising the first amino acid, the first growth-converting factor analog, a first peptide, and a vitamin It may be a mixture of aqueous solutions.

The freeze-drying of the first mixed aqueous solution includes, for example, preparing a frozen product by pre-freezing at a temperature of -35 to -45°C for 5 to 7 hours; and drying the frozen material for 3 to 40 hours while increasing the temperature sequentially in a temperature range of -30 to 35° C.;

For example, the cosmetic composition may be a combination of the first agent and the second agent.

A cosmetic composition in an aqueous solution state is prepared by adding and dissolving the second formulation, which is an aqueous solution, into the solid first formulation. In the cosmetic composition, 80 to 100wt%, 80 to 100wt%, 85 to 100wt%, 90 to 100wt%, 95 to 100wt%, 98 to 100wt% of the first agent within 1 minute after compounding with the second agent at 25°C can be dissolved. In the cosmetic composition, the time for the first agent to completely dissolve after mixing with the second agent at 25° C. is within 1 minute, within 55 seconds, within 50 seconds, within 45 seconds, within 40 seconds, within 35 seconds, or within 30 seconds. may be within

When the first agent has such improved solubility, skin absorption is improved, and side effects such as keratin production can be prevented. If the time for dissolving the first agent is too long, the viscosity of the cosmetic composition increases and stickiness increases, and after application to the skin and drying of the cosmetic composition, a film is formed on the skin surface, and then the film is cracked.

The average osmotic pressure of the cosmetic composition may be, for example, 100 to 1050 mOsmol/kg, 500 to 1045 mOsmol/kg, or 700 to 1042 mOsmol/kg. The cosmetic composition can be absorbed more effectively into the skin by having such a low osmotic pressure. The osmotic pressure can be measured, for example, by the method disclosed in Evaluation Example 5.

The average injection force of the cosmetic composition may be 0.1 to 1.50 N, 0.2 to 1.40 N, 0.5 to 1.30 N, 0.7 to 1.20 N or less, 1.0 to 1.15 N or less. The cosmetic composition can penetrate the skin more effectively by having such a low injection force. The average injection force may be measured, for example, by the method disclosed in Evaluation Example 5.

The cosmetic composition may have a viscosity at 25° C. of 1 to 100 cps, 5 to 90 cps or less, 10 to 85 cps, 15 to 80 cps, or 20 to 75 cps. Since the cosmetic composition has such a low viscosity, it is possible to provide an improved feeling of use without stickiness. In addition, the cosmetic composition can be more effectively absorbed into the skin by having such a low viscosity. The viscosity can be measured, for example, by the method disclosed in Evaluation Example 5.

The cosmetic composition manufacturing method according to another embodiment comprises a sodium hyaluronate salt, a first nucleic acid, a first amino acid, a first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) analog, a first peptide, and a vitamin. providing a solid phase first formulation comprising; providing a liquid phase second formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide; and preparing a cosmetic composition by combining the first agent and the second agent, wherein the sodium hyaluronate included in the first agent is a low molecular weight first sodium hyaluronate salt, and a medium molecular weight second sodium hyaluronate sodium salt. It includes a salt, and a high molecular weight third sodium hyaluronate salt, wherein the content of the third high molecular weight sodium hyaluronate salt is 15 to 25 wt% based on the total weight of the sodium hyaluronate salt.

For example, a solid phase preparation comprising sodium hyaluronate, a first nucleic acid, a first amino acid, a first TGF-β2, Tranforming Growth Factor-β2 analog, a first peptide, and a vitamin 1 formulation is provided.

Also provided is a liquid phase second formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide. The order in which the first agent and the second agent are provided may be selected according to required conditions.

The step of providing a solid first formulation comprising a sodium aluuronate, a first nucleic acid, a first amino acid, a first TGF-β2, Tranforming Growth Factor-β2 analog, a first peptide, and a vitamin is an example For example, to prepare a first mixed aqueous solution comprising a sodium hyaluronate, a first nucleic acid, a first amino acid, a first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) analog, a first peptide, and a vitamin step; and freeze-drying the first mixed aqueous solution.

First, a first mixed aqueous solution containing sodium hyaluronate, a first nucleic acid, a first amino acid, a first transforming growth factor (TGF-β2, Tranforming Growth Factor-β2) analog, a first peptide, and a vitamin is prepared.

The step of preparing the first mixed aqueous solution may include, for example, a first aqueous solution containing a low molecular weight first sodium hyaluronate salt, a second aqueous solution containing the middle molecular weight second sodium hyaluronate salt, and a third high molecular weight solution Preparing a third aqueous solution containing sodium hyaluronate, respectively; preparing a fourth aqueous solution containing a second medium molecular weight sodium hyaluronic acid salt and a first nucleic acid; Preparing a fifth aqueous solution comprising a first amino acid, a first growth-converting factor analog, a first peptide, and a vitamin; and preparing a first mixed aqueous solution by mixing the first aqueous solution, the second aqueous solution, the third aqueous solution, the fourth aqueous solution, and the fifth aqueous solution.

For example, a first aqueous solution containing a low molecular weight sodium hyaluronate is prepared by adding a first sodium hyaluronate salt to distilled water and stirring. A second aqueous solution containing a second sodium hyaluronic acid salt of medium molecular weight is prepared by adding a second sodium hyaluronic acid salt of medium molecular weight to distilled water and stirring. A third aqueous solution containing the high molecular weight third sodium hyaluronate is prepared by adding the third high molecular weight sodium hyaluronate to distilled water and stirring. In addition, a fourth aqueous solution containing the second sodium hyaluronic acid salt and the first nucleic acid of medium molecular weight is prepared by adding the second sodium hyaluronate salt and the first nucleic acid to distilled water and stirring. Prepare a fifth aqueous solution containing the first amino acid, the first growth-converting factor analog, the first peptide, and the first amino acid, the first growth-converting factor analog, the first peptide, and the vitamin by adding and stirring the first amino acid, the first growth-converting factor analog, the first peptide, and the vitamin . The content of each component included in the first aqueous solution to the fifth aqueous solution may be selected according to the required composition of the first agent. The order and method in which the first aqueous solution to the fifth aqueous solution are prepared may be selected according to required conditions. Next, the prepared first aqueous solution, the second aqueous solution, the third aqueous solution, the fourth aqueous solution and the fifth aqueous solution are mixed and stirred to prepare a first mixed aqueous solution.

Next, the prepared first mixed aqueous solution is freeze-dried.

The components included in the first mixed aqueous solution, such as nucleic acids and peptides, are sensitive to the external environment, and since denaturation occurs in a fairly narrow temperature range, the properties of nucleic acids and peptides change when sterilization is performed by increasing the temperature. Therefore, the original effect is insignificant or ineffective. Therefore, freeze-drying of the first mixed aqueous solution is performed in order to prevent structural changes and changes in physical properties of nucleic acids and peptides, etc. and to increase the effect they impart to the skin. Before freeze-drying, a sterilization operation (filtering) of the first mixed aqueous solution may be additionally performed.

The freeze-drying of the first mixed aqueous solution may include, for example, preparing a frozen product by pre-freezing at a temperature of -35 to -45°C for 5 to 7 hours; and drying the frozen material for 3 to 40 hours while sequentially increasing the temperature in a temperature range of -30 to 35°C.

First, a frozen product is prepared by pre-freezing at a temperature of -35 to -45° C. for 5 to 7 hours to prepare a frozen product. The pre-freezing temperature may be, for example, -40°C. The pre-freezing time may be, for example, 6 hours. By freezing in this temperature range and time range, it is possible to more effectively prevent deformation of the components included in the first mixed aqueous solution. Then, the frozen material is dried for 3 to 40 hours while increasing the temperature sequentially in a temperature range of -30 to 35°C. For example, 120-240 minutes at -30°C, 180-300 minutes at -20°C, 0-480 minutes at -15°C, 0-480 minutes at -10°C, 0-360 minutes at -8°C, - 240 to 480 minutes at 5° C., 300 to 420 minutes at 0° C., 0 to 300 minutes at 10° C., 0 to 510 minutes at 15° C., 0 to 300 minutes at 25° C., 120 to 240 minutes at 35° C. You can keep it until completely dry. The time required for total freeze drying may be less than 2000 minutes to 5000 minutes, 2300 minutes to 4950 minutes, or 2,380 minutes to 4,920 minutes. Since the first preparation is prepared in the form of a solid obtained by freeze-drying, deterioration by oxidation reaction of components sensitive to moisture and external environment can be prevented, and the effect can be maintained even after long-term storage. The first agent can be stored, for example, in an inert atmosphere. The inert atmosphere may be, for example, nitrogen, argon, or the like. As the first formulation is stored in an inert atmosphere, deterioration due to oxidation between components sensitive to the external environment and oxygen can be prevented.

Next, a cosmetic composition is prepared by combining the first agent and the second agent.

According to another embodiment, the cosmetic method comprises the steps of providing a cosmetic composition prepared by the above-described method; and applying the provided cosmetic composition to the skin of at least one of the face and neck.

By applying the above-described cosmetic composition to the face, neck, etc., skin elasticity of the face and neck can be improved, moisturizing power can be improved, and skin aging can be prevented. As a result, it is possible to provide the effect of beautifying the skin.

The present invention will be described in more detail through the following specific examples. The following examples are merely examples to help the understanding of the present invention, and the scope of the present invention is not limited thereto.

(Preparation of cosmetic composition)

Example 1: 1st, 2nd, 3rd hyaluronic acid sodium salt weight ratio = 2:6:2

The first aqueous solution in which the first low molecular weight sodium hyaluronate salt was added to purified water, the second aqueous solution in which the second medium molecular weight sodium hyaluronate salt was added to purified water, and the third aqueous solution in which the third high molecular weight sodium hyaluronate salt was added to purified water. were prepared respectively.

The weight average molecular weight of the low molecular weight first sodium hyaluronate salt is 0.15 MDa to less than 0.5 MDa, the weight average molecular weight of the second sodium hyaluronate medium molecular weight is 0.5 MDa to less than 1.3 MDa, and the weight average of the high molecular weight third sodium hyaluronate salt The molecular weight was between 1.3 MDa and less than 1.8 MDa. The weight average molecular weight of the sodium hyaluronate salt was measured with respect to a standard sample using GPC (Gel Permeation Chromatography).

A fourth aqueous solution was prepared by adding the second sodium salt of hyaluronic acid and the first nucleic acid to the purified water.

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution is 2:6:2 It was.

A fifth aqueous solution was prepared by adding the first amino acid, the first transforming growth factor analog, the first peptide, and the vitamin to purified water.

The first aqueous solution, the second aqueous solution, the third aqueous solution, the fourth aqueous solution, and the fifth aqueous solution were mixed, and other mixed components were added to prepare a first mixed aqueous solution. The composition of the first mixed aqueous solution is shown in Table 1 below.

The first mixed aqueous solution was freeze-dried to prepare a solid first formulation. The freeze-drying conditions are shown in Table 2 below. The total freezing and drying time was 2400 min. A first formulation which is a freeze-dried product is shown in FIG. 1 .

A second formulation was prepared by adding a second nucleic acid, a second amino acid, a second transforming growth factor analog, a second peptide, an extract/filtrate and other additives to purified water. The composition of the second formulation is shown in Table 3 below.

The second agent was added to the first agent at 25° C. and mixed to dissolve the second agent and a cosmetic composition was prepared. The cosmetic composition is shown in FIG. 2 .

Example 2: Weight ratio of first, second, and third sodium hyaluronate = 3:5:2

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution is 3:5:2 A cosmetic composition was prepared in the same manner as in Example 1, except that it was changed to . The composition of the first mixed aqueous solution is shown in Table 1 below.

Example 3: Weight ratio of first, second, and third sodium hyaluronate = 4:4:2

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution is 4:4:2 A cosmetic composition was prepared in the same manner as in Example 1, except that it was changed to . The composition of the first mixed aqueous solution is shown in Table 1 below.

Comparative Example 1: Weight ratio of first, second, and third sodium hyaluronate = 3.33:3.33:3.33

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution was 3.33:3.33:3.33 A cosmetic composition was prepared in the same manner as in Example 1, except that the composition of the other ingredients was changed, the freeze-drying conditions were changed, and the composition of the second formulation was changed. The composition of the first mixed aqueous solution is shown in Table 1 below. The freeze-drying conditions are shown in Table 2 below. The composition of the second formulation is shown in Table 3 below.

Comparative Example 2: Weight ratio of 1, 2, 3 sodium hyaluronate = 5:1:4

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution is 5:1:4 to prepare a cosmetic composition in the same manner as in Example 1, except that the freeze-drying conditions were changed. The composition of the first mixed aqueous solution is shown in Table 1 below. The freeze-drying conditions are shown in Table 2 below.

Comparative Example 3: Weight ratio of the first, second, and third sodium hyaluronate = 1.3:8.4:1.3

The weight ratio of the first sodium hyaluronate included in the first aqueous solution, the second sodium hyaluronate included in the second aqueous solution and the fourth aqueous solution, and the third sodium hyaluronate included in the third aqueous solution is 1.3:8.4:1.3 to prepare a cosmetic composition in the same manner as in Comparative Example 1, except that the freeze-drying conditions were changed.

Example 1
[wt%] Example 2
[wt%] Example 3
[wt%] Comparative Example 1
[wt%] Comparative Example 2
[wt%] Comparative Example 3
[wt%] Purified water distilled water remaining amount remaining amount remaining amount remaining amount remaining amount remaining amount Hyaluronic acid sodium salt Hyaluronic acid sodium salt
(Low molecular weight first sodium hyaluronic acid salt: medium molecular weight second sodium hyaluronic acid salt: high molecular weight third sodium hyaluronic acid salt) 0.85
(2:6:2) 0.85
(3:5:2) 0.85
(4:4:2) 0.85 (3.33: 3.33: 3.33) 0.85
(5:1:4) 0.85
(1.3:8.4:1:3) first nucleic acid Sodium DNA 0.001 0.001 0.001 - 0.001 0.001 primary amino acid Glycine 0.09 0.09 0.09 - 0.09 0.09 primary amino acid proline 0.08 0.08 0.08 - 0.08 0.08 primary amino acid Lysine 0.02 0.02 0.02 - 0.02 0.02 primary amino acid Leucine 0.01 0.01 0.01 - 0.01 0.01 first transforming growth factor analog Acetyl sh-Oligopeptide-29 SP 0.00002 0.00002 0.00002 - 0.00002 0.00002 first transforming growth factor analog Acetyl sh-Oligopeptide-30 SP 0.000035 0.000035 0.000035 - 0.000035 0.000035 first transforming growth factor analog Acetyl H-Hexapeptide-8SP
(Acetyl sh-Hexapeptide-8 SP) 0.000045 0.000045 0.000045 - 0.000045 0.000045 first peptide SH-oligopeptide-5
(sh-Oligopeptide-5) 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 vitamin Cyanocobalamine
(Cyanocobalamin) 0.001 0.001 0.001 - 0.001 0.001 other blended ingredients
(preservatives, surfactants, moisturizers, antioxidants) 1,2-Hexanediol, Magnesium Ascorbyl Phosphate, Mannitol, Acetylcysteine, Butylene Glycol 0.5 or less 0.5 or less 0.5 or less 0.5 or less 0.5 or less 0.5 or less Sum 100 100 100 100 100 100

Temperature
[℃] Condition Example 1
[minute] Comparative Example 1
[minute] Comparative Example 2
[minute] -40 freezing 360 360 360 -30 dry 180 180 180 -20 dry 240 -15 dry - 240 420 -10 dry 300 - - -8 dry - 300 - -5 dry 300 300 420 0 dry 360 360 360 10 dry 240 240 - 15 dry - - 480 25 dry 240 240 - 35 dry 180 180 180

Example 1
[wt%] Example 2
[wt%] Example 3
[wt%] Comparative Example 1
[wt%] Comparative Example 2
[wt%] Purified water distilled water remaining amount remaining amount remaining amount remaining amount remaining amount second nucleic acid sodium DNA 0.1 0.1 0.1 - 0.1 second amino acid Glycine 0.09 0.09 0.09 - 0.09 second amino acid proline 0.08 0.08 0.08 - 0.08 second amino acid Lysine 0.02 0.02 0.02 - 0.02 second amino acid Leucine 0.01 0.01 0.01 - 0.01 Second transforming growth factor analog Acetyl sh-Oligopeptide-29 SP 0.000045 0.000045 0.000045 - 0.000045 Second transforming growth factor analog Acetyl sh-Oligopeptide-30 SP 0.000035 0.000035 0.000035 - 0.000035 Second transforming growth factor analog Acetyl sh-Hexapeptide-8 SP 0.000045 0.000045 0.000045 - 0.000045 second peptide RH-oligopeptide-1
(rh-Oligopeptide-1) 0.000007 0.000007 0.000007 2.14 0.000007 active ingredient Galactomyces Ferment Filtrate
(Galactomyces Ferment Filtrate) One One One 2 One active ingredient Bacillus-Soybean Ferment Extract One One One 2 One active ingredient Centella Asiatica Extract One One One 2 One active ingredient Fomes Officinalis Mushroom Extract One One One 2 One active ingredient Purslane Extract (Portulaca Oleracea Extract) One One One One One active ingredient Bambusa Arundinacea Stem Extract One One One One One active ingredient Honey Extract 2.5 2.5 2.5 1.35 2.5 Other mixed ingredients (preservatives, surfactants, humectants, antioxidants) 1,2-Hexanediol, Magnesium Ascorbyl Phosphate, Mannitol, Acetylcysteine, Butylene Glycol 7 or less 7 or less 7 or less 7 or less 7 or less Sum 100 100 100 100 100

Evaluation Example 1: Solubility Evaluation

The first formulation prepared in Examples 1 to 3 and Comparative Examples 1 to 2, respectively, was added to the second formulation, stirred for 30 seconds, and the content of the first formulation dissolved over time was evaluated, and the results are shown in Table 4 below. indicated.

In Table 4, immediately after mixing is immediately after completion of stirring.

The evaluation criteria are as follows:

5: 20% dissolved, 4: 40% dissolved. 3: 60% dissolved, 4: 80% dissolved, 1: 100% dissolved

Solubility immediately after mixing Solubility after 30 seconds Solubility after 1 minute Example 1 3 One One Example 2 3 2 One Example 3 4 3 2 Comparative Example 1 4 4 3 Comparative Example 2 5 4 3

As shown in Table 4, in the cosmetic compositions of Examples 1 to 3, 80 to 100% of the solid of the first formulation was dissolved within 1 minute. In the cosmetic composition of Example 3, the time for completely dissolving the solid of the first formulation was about 1 minute and 10 seconds.

In contrast, in the cosmetic compositions of Comparative Examples 1 and 2, 60% or less of the first formulation solid was dissolved within 1 minute. In the cosmetic composition of Comparative Example 1, the time for completely dissolving the first formulation solid was about 4 minutes and 30 seconds.

Therefore, the cosmetic composition of Examples has significantly improved solubility with respect to the cosmetic composition of Comparative Examples. Therefore, the unstable components included in the first formulation can be stably applied to the skin before decomposition in the aqueous solution.

Evaluation Example 2: Evaluation of dermal layer elasticity

For the cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2, the elasticity of the dermal layer was evaluated using a skin elasticity meter (Dermal Torque Meter, DTM310, Diastron, UK), and a part of the results is shown in FIG. 3 .

Information on the subject of evaluation is as follows.

21 subjects for initial evaluation, 1 dropout, 20 subjects who completed the evaluation, 21 women by gender, average age 47.6 ± 5.2 years.

The cosmetic composition was used twice a day (9:00 am, 9:00 pm) on the face of the subject to be evaluated, and elasticity in the dermis was evaluated before use, after 2 weeks of use, and after 4 weeks of use.

As shown in FIG. 3 , the cosmetic composition of Example 1 showed improved dermal elasticity compared to Comparative Example 1.

In FIG. 3, the x-axis unit is Ue / Ur.

Ur is the maximum pulled skin displacement value, and Ue is the restored skin displacement value after 0.1 seconds. The higher the Ue / Ur, the higher the elasticity.

Evaluation Example 3: Facial cheek area (apple zone) elasticity evaluation

For the cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2, skin elasticity was evaluated using a cutometer (Cutometer, MPA580, Courage+Khazaka. GmbH, Germany), and a part of the results is shown in FIG. 4 .

Information on the subject of evaluation is as follows.

21 subjects for initial evaluation, 1 dropout, 20 subjects who completed the evaluation, 21 women by gender, average age 47.6 ± 5.2 years.

The cosmetic composition was used twice a day (9:00 am, 9:00 pm) on the facial cheek region of the subject to be evaluated, and the elasticity of the cheek region was evaluated before use, after use for 2 weeks, and after use for 4 weeks.

As shown in FIG. 4 , the cosmetic composition of Example 1 showed improved cheek area elasticity compared to Comparative Example 1.

In FIG. 4, the x-axis unit is R2.

R2 (gross elasticity) is a measure of the skin's ability to recover to its initial state after Ua / Uf is released.

Uf is the maximum displacement of the skin inflated by the vacuum, and Ua is the recovered displacement of the skin after 1 second has elapsed at the normal pressure after the vacuum is released. That is, the higher the R2, the higher the recovery.

Evaluation Example 4: Evaluation of skin resilience

For the cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2, the skin elasticity restoring force was evaluated using a balistometer (BLS780, Diastron, UK), and some of the results are shown in FIG. 5 .

Information on the subject of evaluation is as follows.

21 subjects for initial evaluation, 1 dropout, 20 subjects who completed the evaluation, 21 women by gender, average age 47.6 ± 5.2 years.

The cosmetic composition was used twice a day (9:00 am, 9:00 pm) on the face of the subject to be evaluated, and cheek area elasticity was evaluated before use, immediately after one use, after two weeks of use, and after four weeks of use.

As shown in FIG. 5 , the cosmetic composition of Example 1 showed improved skin elasticity restoration compared to Comparative Example 1.

In FIG. 5 , the x-axis unit is CoR.

CoR is a coefficient of restitution (COR), and the higher the value, the better the repulsion. That is, the elasticity is high.

Evaluation Example 5: Evaluation of the physical properties of the cosmetic composition

For the cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2, osmotic pressure, injection force, and viscosity were measured, and some of the results are shown in Table 5 below.

The osmotic pressure was measured using an osmotic pressure meter (GONOTEC, OSMOMAT 3000-D).

The injection force was measured using a tensile compressor (Mecmesin, BFG200N) after filling a 1mL syringe with a 25G needle attached to the cosmetic composition.

Viscosity was measured using (Brookfield, DV2TRVCJ0).

All measurements were performed at 25°C.

osmotic pressure
(Average)
[mOsmol/kg] injection force
(interval average)
[N] Viscosity
(based on 60rpm)
[cps] Example 1 1042 1.13 71.2 Example 2 1041 1.12 71.0 Example 3 1038 1.10 70.7 Comparative Example 1 1079 1.70 169.5

As shown in Table 5, the cosmetic compositions of Examples 1 to 3 showed lower osmotic pressure, lower injection force, and lower viscosity than the cosmetic composition of Comparative Example 1.

Therefore, the cosmetic composition of Examples 1 to 3 was easier to apply to the skin than the cosmetic composition of Comparative Example 1.

Evaluation Example 6: Evaluation of skin irritation

For the cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2, skin irritation was evaluated by the following method.

Information on the subject of evaluation is as follows.

34 subjects for initial evaluation, 1 dropout, 33 subjects who completed evaluation, 34 females by gender, average age 43.6 ± 10.2 years old.

A patch containing the cosmetic composition was attached to the facial skin of the subject to be evaluated, and the patch was removed after 1 hour and 24 hours, respectively, and skin irritation was evaluated. The skin irritation index was calculated after determining the degree of skin irritation according to the criteria of the International Contact Dermatitis Research Group (ICDRG).

As a result of the evaluation, the skin irritation index was 0 in all cosmetic compositions, and there was no skin irritation.

The evaluation criteria of the skin irritation index are as follows:

0~0.25: non-irritant, 0.26~1.0: mildly irritant, 1.01~2.5: moderately irritant, 2.51~4.0: strong irritant

Evaluation Example 7: Sensory Evaluation

The cosmetic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 2 were sensorily evaluated. The evaluation results are shown in Table 6 below.

Information on the subject of evaluation is as follows.

34 subjects for initial evaluation, 1 dropout, 33 subjects who completed evaluation, 34 females by gender, average age 43.6 ± 10.2 years old.

After using the cosmetic composition on the facial skin of the subject to be evaluated, sensory evaluation was performed for the color of the cosmetic composition, the smell of the cosmetic composition, moisturizing power (skin moisture), absorption power (skin elasticity), and stickiness (skin stickiness).

In addition, the sense of use was sensory-evaluated with respect to the overall sense of satisfaction in use in consideration of the above evaluation factors.

The evaluation criteria are as follows: 5: Very good, 4: Excellent, 3: Average, 2; Bad, 1: Very Bad

In Table 6, the lowest stickiness is very good (5). In Table 6, when the skin elasticity is the best, the absorption is very good (5).

moisturizing power absorptivity stickiness Example 1 3 4 4 Example 2 2.5 3 3 Example 3 3 3 3 Comparative Example 1 4 3 2.5 Comparative Example 2 3 2.5 2.5 Comparative Example 3 2.0 2.0 4.5

As shown in Table 6, the cosmetic compositions of Examples 1 to 3 were overall good in all evaluation criteria.

The cosmetic compositions of Comparative Examples 1 and 2 had good moisturizing power, but their sensory evaluation was poor due to high stickiness.

As for the cosmetic composition of Comparative Example 3, the skin was dry due to poor moisturizing power, so the absorption power was also poorly evaluated, and thus the overall feeling of use was poorly evaluated.

As mentioned above, although embodiments of the present invention have been described with reference to the accompanying drawings, those of ordinary skill in the art to which the present invention pertains can practice the present invention in other specific forms without changing its technical spirit or essential features. You will understand that there is Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (20)

a first solid phase preparation comprising sodium hyaluronate, a first nucleic acid, a first amino acid, a first Tranforming Growth Factor (TGF) analog, a first peptide, and a vitamin; and
A second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second liquid phase second formulation comprising a second peptide;
The sodium hyaluronate included in the first formulation includes a low molecular weight first sodium hyaluronate, a medium molecular weight second sodium hyaluronate, and a third high molecular weight sodium hyaluronate
The sodium hyaluronate included in the first agent is,
15 to 45 wt% of a low molecular weight first sodium hyaluronate; 35 to 65 wt% of a second hyaluronic acid sodium salt of medium molecular weight; and 15 to 25 wt% of a high molecular weight third sodium hyaluronate salt,
The weight average molecular weight of the low molecular weight first sodium hyaluronate salt is 0.15 MDa to less than 0.5 MDa,
The weight average molecular weight of the second sodium hyaluronate salt of medium molecular weight is 0.5 MDa to less than 1.3 MDa,
The weight average molecular weight of the high molecular weight third sodium hyaluronate salt is 1.3 MDa to less than 1.8 MDa, cosmetic composition. delete delete The cosmetic composition according to claim 1, wherein the first nucleic acid and the second nucleic acid independently comprise at least one selected from DNA sodium salt, DNA, RNA sodium salt and RNA. The cosmetic composition according to claim 1, wherein the weight average molecular weight of the first nucleic acid and the second nucleic acid is 1700 kDa to 2100 kDa. The cosmetic composition according to claim 1, wherein the first amino acid and the second amino acid independently include one or more selected from leucine, glycine, proline, and lysine. The method according to claim 1, wherein the first transforming growth factor analog and the second transforming growth factor analog are, independently of each other, Acetyl sh-Hexapeptide-8 SP, Acetyl SH-oligopeptide A cosmetic composition comprising at least one selected from -29 SP (Acetyl sh-Oligopeptide-29 SP), and Acetyl SH-Oligopeptide-30 SP. The method of claim 1, wherein the first peptide and the second peptide are independently of each other, sh-oligopeptide-5 (sh-Oligopeptide-5), copper tripeptide-1 (Copper Tripeptide-1), acetyl tetrapeptide-11 (Acetyl Tetrapeptide-11), Palmitoyl Tripeptide-1, Palmitoyl Pentapeptide-4, Tripeptide-1, Acetylhexapeptide-8 Hexapeptide-8), Hexapeptide-9, Palmitoyl Tetrapeptide-7, Nonapeptide-1, and RH-oligopeptide-1 (rh-Oligopeptide) -1) A cosmetic composition comprising at least one selected from among. According to claim 1, wherein the vitamin, retinol (vitamin A), thiamine (thiamine, vitamin B1), riboflavin (vitamin B2), nicotinic acid (vitamin B3), carnitine (carnitine, vitamin B4), pantothenic acid ( pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), folic acid (vitamin B9), cobalamine (vitamin B12), ascorbic acid (vitamin C), At least one selected from phylloquinone (vitamin K), menaquinone (vitamin K), and menadione (vitamin K), a cosmetic composition. According to claim 1, wherein the first agent is magnesium ascorbyl phosphate (Magnesium Ascorbyl Phosphate) as a vitamin derivative, mannitol (mannitol) as a sugar derivative, acetyl cysteine (acetyl cystein) as an amino acid derivative, a preservative, and a moisturizer A cosmetic composition further comprising one or more selected. The cosmetic composition of claim 1, wherein the first agent further comprises at least one selected from 1,2-hexanediol and butylene glycol. The cosmetic composition according to claim 1, wherein the second preparation further comprises one or more active ingredients selected from a plant extract, a ferment extract, a fermentation filtrate, and a honey extract. The method according to claim 1, wherein the second agent is Galactomyces Ferment Filtrate, Bacillus-Soybean Ferment Extract, Centella Asiatica Extract, and Fomes Officinalis Mushroom Extract), purslane extract (Portulaca Oleracea Extract), and thorn bamboo stem extract (Bambusa Arundinacea Stem Extract) further comprising one or more active ingredients selected from, a cosmetic composition. The cosmetic composition according to claim 1, wherein the second agent further comprises one or more additives selected from a moisturizer, a surfactant, an antioxidant, and a preservative. The method of claim 1, wherein the solid first formulation is
Hyaluronic acid sodium salt, a first nucleic acid, a first amino acid, a first transforming growth factor (TGF, Tranforming Growth Factor) analog, a first peptide, and a lyophilized product of a first mixed aqueous solution comprising vitamins, a cosmetic composition. The method of claim 15, wherein the first mixed aqueous solution,
a first aqueous solution containing a low molecular weight first sodium hyaluronate salt, a second aqueous solution containing the second medium molecular weight sodium hyaluronate salt, and a third aqueous solution containing the high molecular weight third sodium hyaluronate salt;
a fourth aqueous solution comprising a second medium-molecular weight sodium salt of hyaluronic acid and a first nucleic acid; and
A cosmetic composition comprising a first amino acid, a first transforming growth factor analog, a first peptide, and a fifth aqueous solution comprising a vitamin. 16. The method of claim 15, wherein the freeze-dried product
preparing a frozen product by pre-freezing at a temperature of -35 to -45° C. for 5 to 7 hours; and
A cosmetic composition prepared by; drying the frozen material for 3 to 40 hours while increasing the temperature sequentially in a temperature range of -30 to 35°C. The cosmetic composition according to claim 1, wherein in the cosmetic composition, 80 to 100 wt% of the first agent is dissolved within 1 minute after blending with the second agent at 25°C. The cosmetic composition according to claim 1, wherein the cosmetic composition has an osmotic pressure of 1050 mOsmol/kg or less, an injection force of 1.50 N or less, and a viscosity at 25°C of 100 cps or less. 20. A method for preparing a cosmetic composition according to any one of claims 1 and 4 to 19, comprising:
Providing a solid phase first formulation comprising a sodium hyaluronate, a first nucleic acid, a first amino acid, a first Tranforming Growth Factor (TGF) analog, a first peptide, and a vitamin;
providing a liquid phase second formulation comprising a second nucleic acid, a second amino acid, a second transforming growth factor analog, and a second peptide; and
Comprising the step of preparing a cosmetic composition by combining the first agent and the second agent,
The sodium hyaluronate included in the first formulation includes a low molecular weight first sodium hyaluronate salt, a medium molecular weight second sodium hyaluronate salt, and a high molecular weight third sodium hyaluronate salt,
The content of the high molecular weight third sodium hyaluronate salt is 15 to 25 wt% based on the total weight of the sodium hyaluronate salt, a cosmetic composition manufacturing method.

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