KR20180060693A - Cosmetic composition having improved skin wrinkle elimination and skin elasticity - Google Patents

Abstract

The present invention relates to a cosmetic composition having improved wrinkle reduction effects and elasticity improvement effects. To this end, the cosmetic composition contains a culture solution obtained by culturing perivascular stem cells derived from human and polydeoxyribonucleotide. According to the present invention, it is possible to prevent wrinkle formation on skin of a user, reduce formed wrinkles, and improve skin elasticity.

Description

Technical Field [0001] The present invention relates to a cosmetic composition having improved skin wrinkle removal and elasticity improvement effect,

The present invention relates to a cosmetic composition having remarkably improved effect of removing wrinkles and improving elasticity. More specifically, the present invention relates to a cosmetic composition in which perivascular stem cells derived from a human are cultured and polydioxyribonucleotides, The present invention relates to a cosmetic composition capable of suppressing the generation of wrinkles and promoting skin elasticity.

Cosmetics have been developed with greatest interest in improving wrinkles caused by skin aging, improving whitening by pigmentation, regenerating skin to improve skin elasticity, and moisturizing to improve skin dryness.

This is in line with the recent development of medical technology and the aging society, and the desire for beauty and beauty is increasing. There is also a lot of research and development for preventing and delaying the aging skin. There is a relationship.

Recently, various researches and developments have been conducted on cosmetic researches for the purpose of preventing aging of skin and keeping young skin, and cosmetic compositions containing various ingredients are being developed.

Recent advances in molecular biology have led to a variety of physiological studies of aging and signaling elements such as growth factors have been found in the human body and these small signaling substances have been shown to decrease with age (Choi, S. W, Korean J. Invest. Dermatol, 1998, 5: 16-20.).

As the age increases, the reduction of these growth factors is highly related to the aging of the human body, and the growth factors are produced in the stem cells that are present at the base of each tissue.

In recent years, techniques for producing cosmetics using stem cells have been developed, but these cosmetics have limitations in which growth factors are not sufficiently contained.

Therefore, there is an urgent need to develop a novel cosmetic composition which can directly and effectively supplement growth factors to the skin.

Patent Document 1: Korean Patent No. 10-0390529 Patent Document 2: Korean Patent No. 10-0986603 Patent Document 3: European Patent No. EP1952798 A1 Patent Document 4: Korean Patent No. 10-1279870 Patent Document 5: Korean Patent No. 10-1321346

Accordingly, the present invention has been made to solve the above problems, and it has been found that a cosmetic composition having remarkably improved skin wrinkle removal and elasticity improvement effect can be prepared using culture medium and polydioxyribonucleotide cultured from human-derived perivascular stem cells , The present invention has been completed on the basis thereof.

The cosmetic composition according to one embodiment of the present invention comprises a culture medium in which human-derived perivascular stem cells are cultured; And polydioxyribonucleotides.

In the cosmetic composition according to another embodiment of the present invention, the culture liquid or the polydioxyribonucleotide may be contained in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition.

In the cosmetic composition according to another embodiment of the present invention, the stem cells may be stem cells collected from cord blood.

In the cosmetic composition according to another embodiment of the present invention, the culture liquid may contain a growth factor.

In a cosmetic composition according to another embodiment of the present invention, the growth factor is selected from the group consisting of transforming growth factor (TGF-beta), insulin-like growth factor (IGF), fibroblast growth factor (FGF) and vascular endothelial growth factor ≪ / RTI >

In the cosmetic composition according to another embodiment of the present invention, the culture liquid may be one cultured by a three-dimensional (3-dimensional) culture method.

The cosmetic composition according to the present invention comprises a culture solution in which perivascular stem cells derived from a human are cultured and a polydioxyribonucleotide, thereby preventing skin wrinkles from being generated by the user, removing wrinkles and enhancing skin elasticity . Further, the cosmetic composition according to the present invention has an advantage that human safety is ensured by using a human stem cell culture solution. The cosmetic composition according to the present invention also has an advantage of improving the skin moisturizing effect.

Before describing the invention in more detail, it is to be understood that the words or words used in the specification and claims are not to be construed in a conventional or dictionary sense, It should be interpreted as meaning and concept consistent with the technical idea of the present invention. Therefore, the constitution of the embodiments described in the present specification is merely a preferred example of the present invention, and does not represent all the technical ideas of the present invention, so that various equivalents and variations And the like.

Hereinafter, preferred embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention.

The present invention relates to a cosmetic composition having improved skin wrinkle removing and elasticity improving effects, and more specifically, it relates to a cosmetic composition comprising a culture solution obtained by culturing human-derived perivascular stem cells and a polydioxyribonucleotide, And to improve the skin elasticity by removing wrinkles.

The cosmetic composition according to the present invention basically comprises a culture solution in which human-derived perivascular stem cells are cultured and a polydioxyribonucleotide.

The structure of the skin is divided into the epidermis on the outer layer which contacts the outside, the dermis which is the layer between the epidermis and the subcutaneous tissue, and the subcutaneous tissue on the lower layer. .

The epidermis can be divided into stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale from the outside. The dermis, which is present below the basal layer of the epidermis and occupies the largest portion of the skin, consists of blood bessel, collagen and elastin fibers, sweat glands, fibroblast ), And macrophages, hair follicles, sebaceous glands, arrector pili muscles, and various sensory nerves.

As people get older, the thickness of the epidermis, dermis, and subcutaneous tissues becomes thinner through aging of the skin, and the formation of wrinkles and the decrease of elasticity due to the deterioration of the extracellular matrix (ECM) Which is characterized by aging of the skin.

Collagen, a structural protein that accounts for one-third of body protein and accounts for 70% to 80% of the dermis dry weight, significantly degrades its ability to regenerate as it ages, affecting important skin structures. Resulting in wrinkle formation.

Collagen in skin connective tissue is biosynthesized in fibroblast and placed on the dermis layer, so it plays a structural role in the dermis and breaks down to collagen life. Metabolism, which is repeated in biosynthesis, biodegradation, biosynthesis, and biodegradation, which is experienced by almost all cells in the human body, continues during the life of the human body. However, as the human body grows older, It has been reported that the human body is progressing with aging.

This is because the collagen of the structural protein is also the same, and as the body ages, collagen biosynthesis capability of the fibroblast gradually decreases, and biodegradation occurs rapidly, and collagen content is reduced and recognized as a main cause of wrinkles of the skin (Parrado, J., M. Bougria, A. Ayala, A. Castano, and A. Machado, Free Radical Biology & Medicine, 1999; 26: 362-370).

In addition to these causes, skin aging is also associated with aging of elastin, fibronectin, glucosaminoglycan (GAG), proteoglycans, laminin, It is known that the function is lost and the elasticity of the skin is decreased.

The external environment also has a great influence on the aging of the human body. It is exposed to exposure to harmful external environments such as daylight and chemicals due to the development of modern arts, and is exposed to excessive stress, Reducing elasticity and accelerating the deepening of wrinkles are interfering with the skin's ability to maintain elasticity and flexibility.

Stem cells are also called pluripotent cells. They are not differentiated into differentiated cells, but need to differentiate into all kinds of cells, such as blood, cartilage, and nerves. ≪ / RTI >

Stem cells can be divided into two types: embryonic stem cells obtained from embryos derived from embryos and adult stem cells obtained from adult stem cells. .

The use of embryonic stem cells is difficult to obtain ethical issues and large numbers of cells, whereas adult stem cells are medically safe, and autologous transplantation does not result in immune rejection, and tissue- And has the advantage of having the ability to store and reproduce stem cells, and various researches and developments have been made recently.

A biocompatible material containing a polymer of deoxyribonucleotide extracted from fish semen, testes and eggs, is composed of a low molecular weight deoxyribonucleotide and a high molecular weight deoxyribonucleotide. The deoxyribonucleotide repeat unit is composed of four kinds of bases , And such a pentose is a biomolecule composed of a phosphodiester bond connected to phosphoric acid and each repeating unit is linked by phosphoric acid to form a polymer. Polydioxyribonucleotides are known to stimulate A2 purinergic receptors to increase the proliferation of epithelial cells and the maturation of extracellular matrix, thereby promoting skin regeneration without side effects, and are known to be effective for burns and chronic wounds. Small molecules of polydioxyribonucleotides are known to be nucleotides, And nucleosides promote the growth of various types of cells, promote the synthesis of nucleic acids, accelerate wound healing, and increase the vascular endothelial growth factor (VEGF) message to produce neoangiogenesis have.

In general, polydioxyribonucleotides are commercially available biopolymers that are obtained in nature and are extracted from semen, testes and eggs of fish.

Conventional techniques for polydioxyribonucleotides are techniques for extracting and obtaining most of polydioxyribonucleotides. Techniques for obtaining a nucleic acid complex material from a tuna testicle or a method for producing a DNA polymer fragment complex from fish semen or eggs It is mainstream.

The use of such polydioxyribonucleotides is also known as a technique for growing hair by a cosmetic manufacturing technique of a specific use including polydioxyribonucleotides, or an anti-aging and antioxidant functional liquid manufacturing technique for cosmetics.

Techniques for producing an eye drop composition using polydioxyribonucleotides and techniques for treating arthritis have been reported.

Stem cells can be classified into embryonic stem cells obtained from inner cell mass of blastocyst and adult stem cells obtained from bone marrow, blood, fat, umbilical cord blood, etc. In human, embryonic stem cells Is inevitably obtained from the embryo generated from the embryo, and the use of such embryonic stem cells is disadvantageous because of the ethical problem and difficulty in obtaining a large number of cells.

Human adult stem cells can be obtained mainly from bone marrow, fat, umbilical cord blood, etc. In the case of adipose stem cells, liposuction can be obtained incidentally during the procedure, so that a sufficient amount of stem cells can be easily obtained and cultured, The perivascular stem cell (PVSC) adult stem cells obtained from umbilical cord blood or perivascular cells have an advantage that they can be obtained from the umbilical cord of the fetus at birth.

In the case of adult stem cells, the use of adult human adult stem cells may be more safe than the use of stem cells derived from mammals, since it is more safe for animal-derived infections than stem cells derived from mammals, The use of cells has the advantage of being able to increase safety.

The cosmetics used in the human body are mainly used in the early days of mammal-derived stem cells have been used for many techniques, mainly human-derived adult stem cells are the main technology.

The use of a stem cell culture liquid for cosmetics means that the stem cells can be obtained by culturing the stem cells, and the culture solution obtained therefrom includes the transforming growth factor (TGF-β), insulin-like growth factor (IGF) Growth factor (FGF), vascular endothelial growth factor (vEGF), growth factor and the like are contained as an active ingredient, thereby exhibiting effects of improving skin wrinkles and inhibiting skin aging.

Therefore, it is important that the growth factors contained in the stem cell culture liquid, which is effective for improving the wrinkles of the skin and inhibiting the aging of the skin, can be contained in a large amount in the stem cell culture liquid.

When culturing stem cells, generally two-dimensional (2D, two-dimensional) cultivation is mainly used and there is a limit to increase the content of active ingredients.

In order to overcome such limitations, the use of a three-dimensional (3D) culture method can significantly increase the content of the active ingredient than the culture method using the two-dimensional culture.

Polydioxyribonucleotide is a biopolymer obtained in nature, and is mainly extracted from semen, testes and eggs of fish. Such polydioxyribonucleotides are known to have effects of promoting skin regeneration, burning, wound healing, and the like.

In general, conventional technologies for polydioxyribonucleotides are mainly techniques for extracting and obtaining polydioxyribonucleotides, and techniques of production methods obtained from semen or eggs of fish tuna or fish are the mainstream.

In addition, cosmetics manufacturing techniques for growing hair with cosmetics containing such polydioxyribonucleotides or techniques for producing anti-aging and antioxidant functional cosmetic liquids are disclosed.

Techniques for producing an eye drop composition using polydioxyribonucleotides and techniques for treating arthritis have been reported.

Such polydioxyribonucleotides can be used together with stem cell culture fluids to increase the effect of polydioxyribonucleotides on skin wrinkles and skin aging suppression, together with stem cell growth factors.

The present invention is characterized by a cosmetic composition for improving skin wrinkles or improving skin elasticity comprising a stem cell culture solution derived from a human and a polydeoxyribonucleotide as an active ingredient.

The present invention relates to a cosmetic composition for improving skin wrinkles or improving skin elasticity comprising a culture medium of human-derived PBMC stem cell and a polydeoxyribonucleotide as an active ingredient.

The human-derived PBMC stem cell culture solution is a culture solution obtained by culturing PBMC stem cell, and the human-derived PBMC stem cell is not an embryonic stem cell but is an adult stem cell, and the bone marrow, umbilical cord blood And stem cells originating from the bases of blood and adipose tissue, including peripheral blood, but are not necessarily limited thereto.

The active ingredient of the human-derived PBMC stem cell culture solution is a peptide that regulates cell growth, that is, a protein, for example, a transcriptional growth factor (TGF-β), an insulin-like growth factor (IGF) (FGF), vascular endothelial growth factor (vEGF), and the like.

Such human-derived fibrosis stem cell culture fluids contain the above-mentioned growth factors, and the effect of improving skin wrinkles and improving elasticity can be expected.

In the cosmetic composition according to the present invention, the culture liquid or the polydeoxyribonucleotide may be contained in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition.

When the content of the stem cell culture solution is less than 0.1 part by weight, the effect of improving the wrinkles and improving the elasticity of the skin is deteriorated. When the content is more than 20 parts by weight, the manufacturing cost is increased, have.

When the content of the polydioxyribonucleotide is less than 0.1 part by weight, the effect of improving the wrinkles and improving the elasticity of the skin is deteriorated. When the amount of the polydioxyboronucleotide is more than 20 parts by weight, the production cost is increased, which is uneconomical, .

The cosmetic composition of the present invention may further contain various cosmetic ingredients used as a conventional cosmetic composition, and the cosmetic ingredients further included in the cosmetic composition may be a variety of materials that can formulate the cosmetic composition according to the use of the cosmetic.

In the cosmetic composition according to another embodiment of the present invention, the stem cells may be stem cells collected from cord blood.

In the cosmetic composition according to another embodiment of the present invention, the culture liquid may contain a growth factor.

In a cosmetic composition according to another embodiment of the present invention, the growth factor is selected from the group consisting of transforming growth factor (TGF-beta), insulin-like growth factor (IGF), fibroblast growth factor (FGF) and vascular endothelial growth factor ≪ / RTI >

In the cosmetic composition according to another embodiment of the present invention, the culture liquid may be one cultured by a three-dimensional (3-dimensional) culture method.

Hereinafter, a specific process for producing the cosmetic composition according to the present invention will be described with reference to the following examples. Various comparative examples in which the effects of the present invention can be comparatively confirmed will be described. However, the scope of the present invention is not limited to the following examples.

Production Example 1: Preparation of a stem cell culture liquid

Stem cells were isolated from 20 g of human umbilical cord blood and cultured in an incubator maintained at 30 째 C for 3 days inoculated with Gibco 占 medium. The thus-cultured stem cell culture was centrifuged at 1,200 rpm for about 3 minutes, and the culture solution of the supernatant was taken to prepare a stem cell culture solution.

Example 1

1 g of the stem cell culture prepared according to Preparation Example 1 and 1 g of polydioxyribonucleotide extracted from the semen of fish were uniformly mixed in 100 g of Lotion A distributed by the company in the market to prepare a cosmetic composition according to the present invention.

Comparative Example 1

1 g of the stem cell culture prepared according to Preparation Example 1 was uniformly mixed with 100 g of Lotion A distributed by the company in the market to prepare a cosmetic composition according to Comparative Example 1.

Comparative Example 2

1 g of the polydioxyribonucleotide extracted from the semen of fish was uniformly mixed in 100 g of lotion A distributed by the company in the market to prepare a cosmetic composition according to Comparative Example 2. [

Comparative Example 3

100 g of a lotion of Company A distributed in the market was prepared as a cosmetic composition according to Comparative Example 3.

[Skin wrinkle removal and elasticity improvement test]

A total of 160 test subjects were enrolled in each of 40 forty men and 40 for women and 50 for men and 40 for women. The test participants were tested for the improvement of facial skin wrinkles.

The test was conducted in such a manner that the male and female of each age group provided the same sample out of the four samples of Example 1 and Comparative Examples 1 to 3.

On the other hand, the test participants did not provide any information about what ingredients were included in the samples used. The wrinkle degree and the degree of elasticity of the facial skin were measured for all the test participants, and 2g of the cosmetic composition prepared according to Example 1 and Comparative Examples 1 to 3 was applied to the test participants three times a day , Skin wrinkle improvement and skin elasticity were measured after 15 days. The initial measurement data was compared with the measurement data after 15 days to evaluate the degree of wrinkle removal and skin elasticity improvement by 1 to 10 points (1 point: lowest improvement, 10 points: highest improvement) , And the average value of the results is shown in Table 1 below.

division Skin wrinkle removal effect Skin elasticity improvement effect Example 1 9.3 9.5 Comparative Example 1 6.5 6.4 Comparative Example 2 5.6 5.2 Comparative Example 3 4.1 4.5

According to the results of Table 1, the cosmetic composition containing both the culture solution and the polydioxyribonucleotide simultaneously cultured from human-derived perivascular stem cells according to the present invention had a superior skin wrinkle removing effect and skin It can be confirmed that the elasticity improving effect is exhibited.

[Skin safety test]

The skin condition after 15 days of the test participants was checked to observe whether the skin was oscillated or keratinized. The results are shown in Table 2 below.

division Presence or absence of skin rash Skin keratin production Example 1 - - Comparative Example 1 - - Comparative Example 2 - - Comparative Example 3 - -

(+: Rash / keratin occurred, -: rash / keratin does not occur)

As shown in Table 2, no abnormal skin was observed in Example 1 and Comparative Examples 1 to 3.

Therefore, the results in Table 2 can be interpreted as supporting the biosafety of the cosmetic composition according to the present invention.

Claims (6)

A culture medium in which human-derived perivascular stem cells are cultured; And
0.0 > polydioxyclene < / RTI > nucleotide. The method according to claim 1,
Wherein the culture liquid or the polydioxyribonucleotide is contained in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition. The method according to claim 1,
Wherein the stem cells are stem cells collected from cord blood. The method according to claim 1,
Wherein the culture liquid contains a growth factor. The method of claim 4,
Wherein the growth factor is at least one of a transforming growth factor (TGF-beta), insulin-like growth factor (IGF), fibroblast growth factor (FGF) and vascular endothelial growth factor (vEGF). The method according to claim 1,
Wherein the culture liquid is cultured by a three-dimensional (3-dimensional) culture method.