CN105030645A - Composition and preparing method and beauty preparation thereof - Google Patents
Composition and preparing method and beauty preparation thereof Download PDFInfo
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- CN105030645A CN105030645A CN201510581959.3A CN201510581959A CN105030645A CN 105030645 A CN105030645 A CN 105030645A CN 201510581959 A CN201510581959 A CN 201510581959A CN 105030645 A CN105030645 A CN 105030645A
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Abstract
The invention provides a composition and a preparing method and beauty preparation thereof. The composition comprises 30-70 volume parts of cell growth factor solutions, 30-70 volume parts of high-concentration blood platelet plasma, and adipose-derived stem cells with the concentration of 0.1*10<6>-2*10<6> /mL. Compared with the prior art, the composition is used for the beauty preparation, and the cell growth factor is a high-activity factor capable of promoting cell growth and promoting angiogenesis, wound healing and tissue damage repairing; the high-concentration blood platelet plasma is beneficial for binding cells and cell surface receptors and improving release of extracellular matrix protein and has a remarkable effect on removing wrinkles and relieving scars and stains; the adipose-derived stem cells can secrete various activity factors with remarkable biological functions, and the three substances interact to improve the wrinkle removal function of the beauty preparation.
Description
Technical field
The invention belongs to Cosmetic surgery field, particularly relate to a kind of composition and method of making the same, cosmetic formulation.
Background technology
Face aging is the result of soft, sclerous tissues's co-variation of body dough-making powder, cervical region.The factors such as aging face and body constitution, inherited genetic factors, living environment, mental emotion, job specification, nutriture, disease, topical skin care have close relationship.Common people, to after 25 years old, just start to occur old and feeble sign, and the formation of face skin wrinkle is an important characters and indexes.
Face rejuvenation refers to the various measures (comprising profile, dissection and physiological structure etc.) that aging face can be made to recover rejuvenation appearance.Along with growth in the living standard, the requirement of people to makings profile is more and more higher, there has also been better requirement, therefore while pursuit is beautiful to safety, water optoinjection technology due to its Wicresoft, painless, recover the advantage such as fast, effective, receive the favor of the personage that likes to be beautiful.
Water optoinjection technology is under the control of micro computer, uses special injection tool to be picked up by skin by negative pressure system, in the space of negative pressure, preparation is imported to a kind of Therapeutic Method at corresponding position.At present, the mix preparation of the hyaluronic acid (HA) that the formula that water optoinjection mainly uses is folk prescription or hyaluronic acid (HA) and high concentration thrombocyte plasma (PRP).
High concentration thrombocyte plasma is that it is widely used in every field, and extends to the application of face rejuvenation from the product of autologous platelet after purifying and concentrating.Concentrated platelet upon activation, a series of cell growth factor can be discharged, as VEGF, platelet derived growth factor (PDGF), IGF, TGF etc., these factors have all been confirmed in experiment and have controlled cell migration, have sticked, bred, and contribute to by being combined with cell surface receptor, improve the release of extracellular matrix protein, have significant effect to reduce wrinkle, desalination cicatrix mottle.
But existing cosmetic formulation composition is comparatively single, and effect is obvious not.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is to provide a kind of composition and method of making the same, cosmetic formulation, and this cosmetic formulation effect is better.
The invention provides a kind of compositions, comprising:
Cell growth factor solution 30 ~ 70 parts by volume;
High concentration thrombocyte plasma 30 ~ 70 parts by volume;
Fat stem cell 0.1 × 10
6~ 2 × 10
6individual/mL.
Preferably, the concentration of described cell growth factor solution is 1 ~ 40mg/mL.
Preferably, described cell growth factor solution is basic fibroblast growth factor solution.
Present invention also offers a kind of preparation method of compositions, comprising:
By the cell growth factor solution of 30 ~ 70 parts by volume, the high concentration thrombocyte plasma and 0.1 × 10 of 30 ~ 70 parts by volume
6~ 2 × 10
6the fat stem cell mixing of individual/mL, obtains compositions.
Present invention also offers a kind of cosmetic formulation, comprise compositions.
Preferably, the temperature that described cosmetic formulation is preserved is 0 DEG C ~ 8 DEG C; The time limit of preserving is 24h.
Present invention also offers a kind of using method of cosmetic formulation, cosmetic formulation is acted on skin surface; Described enamel comprises compositions.
Preferably, the using dosage of described cosmetic formulation is 0.01 ~ 0.02mL/cm
2.
Preferably, described skin surface is skin of face surface.
The invention provides a kind of composition and method of making the same, cosmetic formulation, said composition comprises cell growth factor solution 30 ~ 70 parts by volume; High concentration thrombocyte plasma 30 ~ 70 parts by volume; Fat stem cell 0.1 × 10
6~ 2 × 10
6individual/mL.Compared with prior art, compositions is used for cosmetic formulation by the present invention, cell growth factor has very strong active prognosis, angiogenesis can be promoted, wound healing and tissue injury repair, high concentration thrombocyte plasma contributes to cell and is combined with cell surface receptor, improve the release of extracellular matrix protein, remarkable efficacy is had to reduce wrinkle, desalination cicatrix mottle, fat stem cell can secrete the multiple active factors with remarkable biological function, and above-mentioned three kinds of matter interactions improve the reduce wrinkle function of enamel.
Accompanying drawing explanation
Fig. 1 is the bar diagram of skin arithmetic mean roughness rate of change;
Fig. 2 is skin elasticity relative change rate bar diagram;
Fig. 3 is the skin photo before experimenter uses the cosmetic formulation obtained in embodiment 2;
Fig. 4 is that experimenter uses the skin photo of cosmetic formulation after one month obtained in embodiment 2;
Fig. 5 is the skin photo before experimenter uses the cosmetic formulation obtained in embodiment 3;
Fig. 6 is that experimenter uses the skin photo of cosmetic formulation after one month obtained in embodiment 3.
Detailed description of the invention
Below in conjunction with the accompanying drawing of the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The invention provides a kind of compositions, comprising:
Cell growth factor solution 30 ~ 70 parts by volume;
High concentration thrombocyte plasma 30 ~ 70 parts by volume;
Fat stem cell 0.1 × 10
6~ 2 × 10
6individual/mL.
Wherein, the content of described cell growth factor solution is preferably 40 ~ 60 parts by volume, then is preferably 45 ~ 55 parts by volume; Described cell growth factor concentration is concentration well known to those skilled in the art, and there is no special restriction, the present invention is preferably 1 ~ 40mg/mL, is more preferably 5 ~ 35mg/mL, then is preferably 10 ~ 30mg/mL; The kind of described cell growth factor is preferably basic fibroblast growth factor.
Basic fibroblast growth factor (bFGF) is distributed widely in tissue. and be a kind of trace active albumen contained in human body.Endotheliocyte is its main source, can discharge bFGF in a large number when endotheliocyte is impaired, and it has promote that silk splits, breaks up and regulating action to coming from mesoderm and neuroectodermal biological cells and tissues. be wound healing and epitheliogenic important instrumentality.Its cosmetic result had has: (1) efficiently repairs: promote fibroblast, epidermis cell metabolism, propagation and differentiation; (2) smooth away wrinkles: the formation promoting intercellular substance, promote synthesis and the secretion of collagen protein, promote elastic fiber synthesis and secretion.Promote the increase of intercellular matrix, make skin delicate vigorous and graceful, full flexible, thus smooth away wrinkles; (3) whitening is ruddy: improve skin microcirculation, ensures that Skin Cell has sufficient nutrition supply, makes wax yellow, matt, unsound skin become ruddy glossy.
The content of described high concentration thrombocyte plasma is preferably 40 ~ 60 parts by volume, then is preferably 45 ~ 55 parts by volume, is more preferably 48 ~ 52 parts by volume.High concentration thrombocyte plasma (PRP) is from the product of autologous platelet after purifying and concentrating.Concentrated platelet upon activation, a series of cell growth factor can be discharged, as VEGF, platelet derived growth factor (PDGF), IGF, TGF, these factors have control cell migration, stick, breed and contribute to by being combined with cell surface receptor, improve the release of extracellular matrix protein, have remarkable efficacy to reduce wrinkle, desalination cicatrix mottle.
The content of described fat stem cell is preferably 0.5 × 10
6~ 1.5 × 10
6individual/mL, is more preferably 0.8 × 10
6~ 1.2 × 10
6individual/mL.Fat stem cell is from fatty tissue, be separated a kind of stem cell with multi-lineage potential obtained, the multiple active factors with remarkable biological function can be secreted, as VEGF, EGF, SDF-1, bFGF, TGF-β 1, VEGF b2 etc., these factors are being improved cardiovascular disease conditions, are being promoted to have irreplaceable effect in tissue regeneration, repair tissue damage, check melanin precipitation etc.
Present invention also offers a kind of preparation method of above-mentioned composition, comprising: by the cell growth factor solution of 30 ~ 70 parts by volume, the high concentration thrombocyte plasma and 0.1 × 10 of 30 ~ 70 parts by volume
6~ 2 × 10
6the fat stem cell of individual/mL mixes, obtains compositions.
Wherein, described cell growth factor solution, high concentration thrombocyte plasma, fat stem cell are all same as above, do not repeat them here.Described cell growth factor solution is mixed to get by cell growth factor lyophilized powder and solvent, and described solvent is solvent well known to those skilled in the art, there is no special restriction, and the present invention is preferably solvent with normal saline.
According to the present invention, preferably first by the high concentration thrombocyte plasma and 0.1 × 10 of 30 ~ 70 parts by volume
6~ 2 × 10
6the fat stem cell mixing of individual/mL, and then mix with the cell growth factor solution of 30 ~ 70 parts by volume, obtain compositions.
Present invention also offers a kind of cosmetic formulation, comprise above-mentioned compositions.The temperature that this cosmetic formulation is preserved is preferably 0 DEG C ~ 8 DEG C; The time limit of preserving is preferably 24h.
Compositions is used for cosmetic formulation by the present invention, cell growth factor can act on the one-tenth fiber stem cell of skin corium, make to be in dormancy, the one-tenth fiber stem cell of damage is activated and repairs and a large amount of fibroblast of ripe differentiation, high concentration thrombocyte plasma contributes to cell and is combined with cell surface receptor, improve the release of extracellular matrix protein, remarkable efficacy is had to reduce wrinkle, desalination cicatrix mottle, fat stem cell can secrete the multiple active factors with remarkable biological function, and above-mentioned three kinds of matter interactions improve the reduce wrinkle function of enamel.
Present invention also offers a kind of using method of above-mentioned cosmetic formulation, cosmetic formulation is acted on skin surface, preferably act on skin subcutaneous tissue, more preferably adopt water optoinjection technology that cosmetic formulation is acted on skin subcutaneous tissue.The using dosage of described cosmetic formulation is preferably 0.01 ~ 0.02mL/cm
2; Described surface skin member is preferably skin of face surface.
In order to further illustrate the present invention, below in conjunction with embodiment, a kind of composition and method of making the same provided by the invention, cosmetic formulation are described in detail.
Reagent used in following examples is commercially available.
Embodiment 1
1.1 get P1 generation, degree of converging reach 90% fat stem cell, remove culture supernatant, after washing 3 times with normal saline, add 0.02mL/cm
20.25% pancreatin and 0.02%EDTA digest 2min, enzymolysis is stopped with 10 times of complete mediums to Digestive system (lonzaUltraCULTUREMEDIUM serum-free medium+PALL serum substitute+2mML-glutamine+1xNEAA), sampling counting, the centrifugal 5min of 200g, remove supernatant, use normal saline re-suspended cell, according to count results, adjustment total cellular score is 5 × 10
6individual, add normal saline to the centrifugal 5min of 45mL, 200g, then clean twice cell according to above-mentioned steps, finally precipitation is the fat stem cell prepared.
1.2 get the centrifugal 10min of 20mL autologous whole blood 400g, get blood plasma, abandon erythrocyte, the centrifugal 5min of 1200g, and retain 2mL blood plasma, remainder abandons, resuspended precipitation, obtains high concentration thrombocyte plasma (PRP).
1.3 with the fat stem cell obtained in the PRP obtained in 1.2 resuspended 2.1, then adds 3mL basic fibroblast growth factor solution (concentration is 10mg/mL) mix homogeneously, obtains cosmetic formulation.
Embodiment 2
2.1 get P2 generation, degree of converging reach 80% fat stem cell, remove culture supernatant, after washing 3 times with normal saline, add 0.02mL/cm
20.25% pancreatin and 0.02%EDTA digest 2min, enzymolysis is stopped with 10 times of complete mediums to Digestive system (lonzaUltraCULTUREMEDIUM serum-free medium+PALL serum substitute+2mML-glutamine+1xNEAA), sampling counting, the centrifugal 5min of 200g, remove supernatant, use normal saline re-suspended cell, according to count results, adjustment total cellular score is 0.5 × 10
6individual, add normal saline to the centrifugal 5min of 45mLmL, 200g, then clean twice cell according to above-mentioned steps, finally precipitation is the fat stem cell prepared.
2.2 get the centrifugal 10min of 20mL autologous whole blood 400g, get blood plasma, abandon erythrocyte, the centrifugal 5min of 1200g, and retain 2.5mL blood plasma, remainder abandons, and gravity treatment precipitates, and obtains high concentration thrombocyte plasma (PRP).
2.3 with the fat stem cell obtained in the PRP obtained in 2.2 resuspended 2.1, add 2.5mL basic fibroblast growth factor solution (concentration is 20mg/mL) mix homogeneously again, obtain cosmetic formulation, be placed in syringe, 4 DEG C of transports, finish using in 1h.
Embodiment 3
3.1 get P1 generation, degree of converging reach 80% fat stem cell, remove culture supernatant, after washing 3 times with normal saline, add 0.02mL/cm
20.25% pancreatin and 0.02%EDTA digest 2min, enzymolysis is stopped with 10 times of complete mediums to Digestive system (lonzaUltraCULTUREMEDIUM serum-free medium+PALL serum substitute+2mML-glutamine+1xNEAA), sampling counting, the centrifugal 5min of 200g, remove supernatant, use normal saline re-suspended cell, according to count results, adjustment total cellular score is 10 × 10
6individual, add normal saline to the centrifugal 5min of 45mLmL, 200g, then clean twice cell according to above-mentioned steps, finally precipitation is the fat stem cell prepared.
3.2 get the centrifugal 10min of 20mL autologous whole blood 400g, get blood plasma, abandon erythrocyte, the centrifugal 5min of 1200g, and retain 3mL blood plasma, remainder abandons, and gravity treatment precipitates, and obtains high concentration thrombocyte plasma (PRP).
3.3 with the fat stem cell obtained in the PRP obtained in 3.2 resuspended 3.1, add 2mL basic fibroblast growth factor solution (concentration is 30mg/mL) mix homogeneously again, obtain cosmetic formulation, be placed in syringe, 4 DEG C of transports, finish using in 1h.
Comparative example 1
With 5mL hyaluronic acid for cosmetic formulation.
Comparative example 2
2.1 get the centrifugal 5min of 20mL autologous whole blood 400g, get blood plasma, abandon erythrocyte, the centrifugal 5min of 1200g, and retain 5mL blood plasma, remainder abandons, and gravity treatment precipitates, and obtains high concentration thrombocyte plasma (PRP).
2.2 PRP and the 0.5mL hyaluronic acid mixing will obtained in 2.1, obtain cosmetic formulation.
Comparative example 3
3.1 get P1 generation, degree of converging reach 90% fat stem cell, remove culture supernatant, after washing 3 times with normal saline, add 0.02mL/cm
20.25% pancreatin and 0.02%EDTA digest 2min, enzymolysis is stopped with 10 times of complete mediums to Digestive system (lonzaUltraCULTUREMEDIUM serum-free medium+PALL serum substitute+2mML-glutamine+1xNEAA), sampling counting, the centrifugal 5min of 200g, remove supernatant, use normal saline re-suspended cell, according to count results, adjustment total cellular score is 8 × 10
6individual, add normal saline to the centrifugal 5min of 45mLmL, 200g, then clean twice cell according to above-mentioned steps, finally precipitation is the fat stem cell prepared.
The fat stem cell obtained in 3.1 mixes with 5mL hyaluronic acid by 3.2, obtains cosmetic formulation.
Comparative example 4
4mL basic fibroblast growth factor solution (concentration is 10mg/mL) is mixed homogeneously with 1mL hyaluronic acid, obtains cosmetic formulation.
Comparative example 5
5.1 get P1 generation, degree of converging reach 90% fat stem cell, remove culture supernatant, after washing 3 times with normal saline, add 0.02mL/cm
20.25% pancreatin and 0.02%EDTA digest 2min, enzymolysis is stopped with 10 times of complete mediums to Digestive system (lonzaUltraCULTUREMEDIUM serum-free medium+PALL serum substitute+2mML-glutamine+1xNEAA), sampling counting, the centrifugal 5min of 200g, remove supernatant, use normal saline re-suspended cell, according to count results, adjustment total cellular score is 8 × 10
6individual, add normal saline to the centrifugal 5min of 45mLmL, 200g, then clean twice cell according to above-mentioned steps, finally precipitation is the fat stem cell prepared.
5.2 with the fat stem cell obtained in 4mL basic fibroblast growth factor solution (concentration is 10mg/mL) resuspended 5.1, then mixs homogeneously with 1mL hyaluronic acid, obtains cosmetic formulation.
Recruit 120 Chinese women experimenters, 30 ~ 60 years old age, be divided into six groups, often organize 20 people, use in embodiment 1 cosmetic formulation that obtains for the A group mean age (44.12 ± 1.91) year, use cosmetic formulation in comparative example 1 for the B group mean age (43.91 ± 1.97) year, use in comparative example 2 cosmetic formulation that obtains for the C group mean age (44.01 ± 2.02) year, use in comparative example 3 cosmetic formulation that obtains for the D group mean age (43.89 ± 1.93) year, usage ratio compared with 4 in the cosmetic formulation that obtains be year E group mean age (43.93 ± 1.99), usage ratio compared with 5 in the cosmetic formulation that obtains be year E group mean age (44.03 ± 2.03).Every experimenter is all apprised of whole research process, and signs Informed Consent Form.Use cosmetic formulation 5mL to six groups of experimenter's faces by water optoinjection technology, Continuous Observation 28 days, period does not use other skin-protection products, normal activity, notes sun-proof.In use after 28 days, the use position of experimenter is cleaned, used the Multi-functional skin mirror image analysis system mensuration of the MicroSkinII model adopting German CK company skin elasticity tester MPA580 and MotieChinaCO.Ltd. company to provide to smear skin elasticity and the stricture of vagina reason at position by tester, survey 5 times for often, average, statistics uses the skin of face situation of change after preparation, obtain the bar diagram of skin arithmetic mean roughness rate of change as shown in Figure 1, the degree of roughness of arithmetic mean roughness reaction skin, be worth lower, skin is more smooth, the rejuvenation effect of preparation is better, as shown in Figure 1, each group all has roughness in various degree to decline, but A group rate of change is maximum, obtain skin elasticity relative change rate bar diagram as shown in Figure 2, skin elasticity relative change rate reacts the change that cosmetic formulation uses rear skin elasticity, and be worth larger, skin elasticity becomes better, and as shown in Figure 2, the cosmetic formulation that A group uses is better than other groups.
The cosmetic formulation obtained in embodiment 2 is tested: experimenter's face uses cosmetic formulation 5mL by water optoinjection technology, and tested period does not use other skin-protection products, normal activity, notes sun-proof.Fig. 3 is the skin photo before using, and Fig. 4 uses the skin photo after latter month, and become bright and clean by contrast visible dermis, microgroove reduces, and the colour of skin is evenly pale.
The cosmetic formulation obtained in embodiment 3 is tested: experimenter's face uses cosmetic formulation 5mL by water optoinjection technology, and tested period does not use other skin-protection products, normal activity, notes sun-proof.Fig. 5 is the skin photo before using, and Fig. 6 uses the skin photo after latter month, and obviously improved by contrast visible dermis, the colour of skin is evenly glossy, and microgroove reduces.
Claims (9)
1. a compositions, is characterized in that, comprising:
Cell growth factor solution 30 ~ 70 parts by volume;
High concentration thrombocyte plasma 30 ~ 70 parts by volume;
Fat stem cell 0.1 × 10
6~ 2 × 10
6individual/mL.
2. compositions according to claim 1, is characterized in that, the concentration of described cell growth factor solution is 1 ~ 40mg/mL.
3. compositions according to claim 1, is characterized in that, described cell growth factor solution is basic fibroblast growth factor solution.
4. a preparation method for compositions, is characterized in that, comprising:
By the cell growth factor solution of 30 ~ 70 parts by volume, the high concentration thrombocyte plasma and 0.1 × 10 of 30 ~ 70 parts by volume
6~ 2 × 10
6the fat stem cell mixing of individual/mL, obtains compositions.
5. a cosmetic formulation, is characterized in that, comprises the compositions described in claims 1 to 3 any one or the compositions prepared by claim 4.
6. according to the cosmetic formulation shown in claim 5, it is characterized in that, the temperature that described cosmetic formulation is preserved is 0 DEG C ~ 8 DEG C; The time limit of preserving is 24h.
7. a using method for cosmetic formulation, is characterized in that, cosmetic formulation is acted on skin surface; Described enamel comprises the compositions described in claims 1 to 3 any one or the compositions prepared by claim 4.
8. using method according to claim 7, is characterized in that, the using dosage of described cosmetic formulation is 0.01 ~ 0.02mL/cm
2.
9. using method according to claim 7, is characterized in that, described skin surface is skin of face surface.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110304A (en) * | 2016-07-28 | 2016-11-16 | 广州赛莱拉干细胞科技股份有限公司 | One is dispelled scar compositions and dressing |
| CN106267353A (en) * | 2016-09-19 | 2017-01-04 | 广州市拜沃思生物科技有限公司 | A kind of fat stem cell filler and preparation method thereof |
| CN106580718A (en) * | 2016-12-29 | 2017-04-26 | 广州远想生物科技有限公司 | Cell growth factor composition and preparation method thereof, beautifying preparation and application |
| CN106727706A (en) * | 2016-12-26 | 2017-05-31 | 湖南新起源医疗技术有限公司 | A kind of preparation method of the cell preparation repaired for skin injury |
| CN109097325A (en) * | 2018-08-01 | 2018-12-28 | 丁彬彬 | A kind of preparation method of the fat stem cell liquid for cosmetology |
| CN109674820A (en) * | 2019-01-25 | 2019-04-26 | 华子昂 | Color spot restorative procedure and application based on stem cell |
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| CN104622771A (en) * | 2015-01-13 | 2015-05-20 | 广州赛奕德生物技术有限公司 | Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof |
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| US20130216626A1 (en) * | 2012-02-17 | 2013-08-22 | Quimera Ingenieria Biomedica, S.L. | Platelet-rich plasma compositions |
| CN104622771A (en) * | 2015-01-13 | 2015-05-20 | 广州赛奕德生物技术有限公司 | Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110304A (en) * | 2016-07-28 | 2016-11-16 | 广州赛莱拉干细胞科技股份有限公司 | One is dispelled scar compositions and dressing |
| CN106267353A (en) * | 2016-09-19 | 2017-01-04 | 广州市拜沃思生物科技有限公司 | A kind of fat stem cell filler and preparation method thereof |
| CN106267353B (en) * | 2016-09-19 | 2019-12-10 | 广州市拜沃思生物科技有限公司 | Adipose-derived stem cell filler and preparation method thereof |
| CN106727706A (en) * | 2016-12-26 | 2017-05-31 | 湖南新起源医疗技术有限公司 | A kind of preparation method of the cell preparation repaired for skin injury |
| CN106580718A (en) * | 2016-12-29 | 2017-04-26 | 广州远想生物科技有限公司 | Cell growth factor composition and preparation method thereof, beautifying preparation and application |
| CN109097325A (en) * | 2018-08-01 | 2018-12-28 | 丁彬彬 | A kind of preparation method of the fat stem cell liquid for cosmetology |
| CN109674820A (en) * | 2019-01-25 | 2019-04-26 | 华子昂 | Color spot restorative procedure and application based on stem cell |
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