CN106267353A - A kind of fat stem cell filler and preparation method thereof - Google Patents
A kind of fat stem cell filler and preparation method thereof Download PDFInfo
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- CN106267353A CN106267353A CN201610831877.4A CN201610831877A CN106267353A CN 106267353 A CN106267353 A CN 106267353A CN 201610831877 A CN201610831877 A CN 201610831877A CN 106267353 A CN106267353 A CN 106267353A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
The invention discloses a kind of fat stem cell filler, including fat stem cell, become fiber stem cell, fibroblast, nutrient for cell growth, fat stem cell, one-tenth fiber stem cell, fibroblastic density are respectively 1 × 107‑5×107Individual/ml, 0.5 × 107‑1×107Individual/ml, 0.5 × 107‑1×107Individual/ml, the concentration of nutrient for cell growth is 20 30mg/ml.Invention additionally discloses the preparation method of a kind of fat stem cell filler.The filler of the present invention, by fat stem cell, one-tenth fiber stem cell, fibroblast, the synergism of nutrient for cell growth, can improve the repairing quality of defective tissue.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of fat stem cell filler and preparation method thereof.
Background technology
Tissue is usually because aging, wound generation defect, and such as: along with the increase at age, evidence for senescence is gradually obvious,
Can there is wrinkle and depression in various degree because of the loss of fat in face, affects face appearance.At present, filling is generally used
The tissue of defect is repaired by agent, and filler is mainly paraffin, silica gel, animal collagen, polyacrylamide gel, transparent
Matter acid gel, cell growth factor etc..
But paraffin, silica gel, animal collagen, polyacrylamide gel are due to non-human Self substances, the row of being susceptible to
Scolding reaction, hyaluronic acid derivatives is decomposed absorption because of metabolism, does not reaches the purpose of long-term filling, and cell growth factor is because of locally
Residence time is relatively brief, does not reaches the purpose of permanent stimulating cellular growth.How to improve the filling effect of filler, for
Defective tissue is repaired, and has important effect.
Summary of the invention
Technical problem is how of present invention mainly solves provides a kind of filler, can improve the reparation matter of defective tissue
Amount.
For solving above-mentioned technical problem, the present invention provides a kind of fat stem cell filler, including fat stem cell, fibroblast
Dimension stem cell, fibroblast, nutrient for cell growth, the density of fat stem cell is 1 × 107-5×107Individual/ml, one-tenth fiber
The density of stem cell is 0.5 × 107-1×107Individual/ml, fibroblastic density are 0.5 × 107-1×107Individual/ml, cell
The concentration of growing nutrient element is 20-30mg/ml.
Wherein, the density of fat stem cell is 3 × 107Individual/ml, the density of one-tenth fiber stem cell are 0.8 × 107Individual/ml,
Fibroblastic density is 0.8 × 107Individual/ml, the concentration of nutrient for cell growth are 25mg/ml.
Wherein, fat stem cell, one-tenth fiber stem cell, fibroblastic density also can be respectively 2 × 107Individual/ml,
0.6×107Individual/ml, 0.6 × 107Individual/ml, the concentration of nutrient for cell growth can be also 25mg/ml.
Wherein, fat stem cell is the third generation to the fat stem cell in the 5th generation.
For solve above-mentioned technical problem, the present invention also provides for the preparation method of a kind of fat stem cell filler, including with
Lower step: be completely dissolved in water by nutrient for cell growth, adds fat stem cell, becomes fiber stem cell, fibroblast,
Resuspended each cell, prepares fat stem cell filler；Wherein, the density of fat stem cell is 1 × 107-5×107Individual/ml, fibroblast
The density of dimension stem cell is 0.5 × 107-1×107Individual/ml, fibroblastic density are 0.5 × 107-1×107Individual/ml, thin
The concentration of intracellular growth nutrient is 20-30mg/ml.
Wherein, the density of fat stem cell is 3 × 107Individual/ml, the density of one-tenth fiber stem cell are 0.8 × 107Individual/ml,
Fibroblastic density is 0.8 × 107Individual/ml, the concentration of nutrient for cell growth are 25mg/ml.
Wherein, fat stem cell, one-tenth fiber stem cell, fibroblastic density also can be respectively 2 × 107Individual/ml,
0.6×107Individual/ml, 0.6 × 107Individual/ml, the concentration of nutrient for cell growth can be also 25mg/ml.
Wherein, fat stem cell is the third generation to the fat stem cell in the 5th generation.
Wherein, fat stem cell obtains in the way of using mixing collagenase digesting.
Wherein, epoxy glue protoenzyme is made up of II Collagenase Type, papain, and concentration during its digestion fatty tissue is
0.1%(g/ml), wherein, epoxy glue protoenzyme 1%(g/ml) collocation method of mother solution is to add in 100ml D-Hank's solution
0.5g II Collagenase Type, 0.5g papain.
The invention has the beneficial effects as follows: be different from the situation of prior art, the fat stem cell filler of the present invention includes
Fat stem cell, one-tenth fiber stem cell, fibroblast, nutrient for cell growth, wherein, fat stem cell, one-tenth fiber are dry thin
Born of the same parents, fibroblastic density are respectively 1 × 107-5×107Individual/ml, 0.5 × 107-1×107Individual/ml, 0.5 × 107-1×
107Individual/ml, the concentration of nutrient for cell growth is 20-30mg/ml.The filler of the present invention is by fat stem cell, one-tenth fiber
Stem cell, fibroblast, the synergism of nutrient for cell growth, can improve the repairing quality of defective tissue.And in preparation
During fat stem cell, digested by the epoxy glue protoenzyme of II Collagenase Type, papain composition so that fatty tissue disappears
Change more thorough, it is thus achieved that fat stem cell purity higher.
Detailed description of the invention
Below by embodiment, the present invention is described in detail.
Embodiment 1
Fat stem cell filler includes fat stem cell, becomes fiber stem cell, fibroblast, nutrient for cell growth, its
In, fat stem cell, one-tenth fiber stem cell, fibroblastic density are respectively 1 × 107-5×107Individual/ml, 0.5 × 107-1
×107Individual/ml, 0.5 × 107-1×107Individual/ml, the concentration of nutrient for cell growth is 20-30mg/ml.
In the present embodiment, fat stem cell, one-tenth fiber stem cell, fibroblastic density are respectively 3 × 107Individual/
ml、0.8×107Individual/ml, 0.8 × 107Individual/ml, the concentration of nutrient for cell growth is 25mg/ml.
In the present embodiment, the preparation method of fat stem cell filler comprises the following steps: by nutrient for cell growth
It is completely dissolved in water, adds fat stem cell, become fiber stem cell, fibroblast, resuspended each cell, prepare fat dry thin
Born of the same parents' filler.
Wherein, during obtaining fat stem cell, the mode of mixing collagenase digesting is used to obtain.Epoxy glue
Protoenzyme is made up of II Collagenase Type, papain, and concentration during its digestion fatty tissue is 0.1%(g/ml).Epoxy glue protoenzyme
1%(g/ml) collocation method of mother solution is: add 0.5g II Collagenase Type, 0.5g Papain in 100ml D-Hank's solution
Enzyme.
The female subjects that 60 ages are 30-60 year being divided into 3 groups, often organizes 20 people, first group uses the present embodiment
Filler, second group uses hyaluronic acid is the filler of component, and the 3rd group uses cell growth factor is the filler of component.
The face of 3 groups of experimenters is used preparation, Continuous Observation 60 days, wrinkle, the skin rating to experimenter.
Wherein, the mean age of each group is 41.2 ± 3.1 years old.
Wherein, grade is divided into 4 grades, 1 grade: facial wrinkles is substantially desalinated, skin smooth, 2 grades: facial wrinkles desalination, skin
Smooth, 3 grades: wrinkle has a little minimizing, skin to make moderate progress, 4 grades: wrinkle without reducing, skin is without improving.
After having tested, the test result of each group is as follows:
First group: 1 grade, 6 people；2 grades, 11 people；3 grades, 2 people；4 grades, 1 people.
Second group: 1 grade, 4 people；2 grades, 9 people；3 grades, 5 people；4 grades, 2 people.
3rd group: 1 grade, 3 people；2 grades, 8 people；3 grades, 7 people；4 grades, 2 people.
Above-mentioned test, the smoothing wrinkle of the present embodiment preparation, skin improve effective percentage apparently higher than the smoothing wrinkle of other preparations, skin
Improve effective percentage.
Select 60 examples to have the female subjects of depressed scar after acne or wound, be divided into 3 groups, often organize 20 people, first
Group uses the filler of the present embodiment, and second group uses hyaluronic acid is the filler of component, and the 3rd group uses cell growth factor
Son is the filler of component.The position need to repaired for experimenter, altogether injection 2 times, every minor tick 2 weeks, each 3ml.
Wherein, the mean age of each group is 41.2 ± 3.1 years old.
After reparation terminates, the depressed scar of first group of whole experimenter substantially shoals, and effect is fine, and second group of part is subject to
The depressed scar of examination person (15) shoals, and the depressed scar of the 3rd group of part experimenter (13) shoals.
Above-mentioned test, the repair ability of the defective tissue of the present embodiment preparation is higher than the reparation energy of other preparation defective tissues
Power.
Embodiment 2
Fat stem cell filler includes fat stem cell, becomes fiber stem cell, fibroblast, nutrient for cell growth, its
In, fat stem cell, one-tenth fiber stem cell, fibroblastic density are respectively 2 × 107Individual/ml, 0.6 × 107Individual/ml, 0.6
×107Individual/ml, the concentration of nutrient for cell growth is 25mg/ml.
In the present embodiment, the preparation method of fat stem cell filler comprises the following steps: by nutrient for cell growth
It is completely dissolved in water, adds fat stem cell, become fiber stem cell, fibroblast, resuspended each cell, prepare fat dry thin
Born of the same parents' filler.
Wherein, during obtaining fat stem cell, the mode of mixing collagenase digesting is used to obtain.Epoxy glue
Protoenzyme is made up of II Collagenase Type, papain, and concentration during its digestion fatty tissue is 0.1%(g/ml).Epoxy glue protoenzyme
1%(g/ml) collocation method of mother solution is: add 0.5g II Collagenase Type, 0.5g Papain in 100ml D-Hank's solution
Enzyme.
The female subjects that 60 ages are 30-60 year being divided into 3 groups, often organizes 20 people, first group uses the present embodiment
Filler, second group uses hyaluronic acid is the filler of component, and the 3rd group uses cell growth factor is the filler of component.
The face of 3 groups of experimenters is used preparation, Continuous Observation 60 days, wrinkle, the skin rating to experimenter.
Wherein, the mean age of each group is 41.2 ± 3.1 years old.
Wherein, grade is divided into 4 grades, 1 grade: facial wrinkles is substantially desalinated, skin smooth, 2 grades: facial wrinkles desalination, skin
Smooth, 3 grades: wrinkle has a little minimizing, skin to make moderate progress, 4 grades: wrinkle without reducing, skin is without improving.
After having tested, the test result of each group is as follows:
First group: 1 grade, 5 people；2 grades, 11 people；3 grades, 3 people；4 grades, 1 people.
Second group: 1 grade, 4 people；2 grades, 9 people；3 grades, 5 people；4 grades, 2 people.
3rd group: 1 grade, 3 people；2 grades, 8 people；3 grades, 7 people；4 grades, 2 people.
Above-mentioned test, the smoothing wrinkle of the present embodiment preparation, skin improve effective percentage apparently higher than the smoothing wrinkle of other preparations, skin
Improve effective percentage.
Select 60 examples to have the female subjects of depressed scar after acne or wound, be divided into 3 groups, often organize 20 people, first
Group uses the filler of the present embodiment, and second group uses hyaluronic acid is the filler of component, and the 3rd group uses cell growth factor
Son is the filler of component.The position need to repaired for experimenter, altogether injection 2 times, every minor tick 2 weeks, each 3ml.
Wherein, the mean age of each group is 41.2 ± 3.1 years old.
After reparation terminates, the depressed scar of first group of whole experimenter substantially shoals, and effect is fine, and second group of part is subject to
The depressed scar of examination person (15) shoals, and the depressed scar of the 3rd group of part experimenter (13) shoals.
Above-mentioned test, the repair ability of the defective tissue of the present embodiment preparation is higher than the reparation energy of other preparation defective tissues
Power.
In sum, the filler of the present invention is by fat stem cell, one-tenth fiber stem cell, fibroblast, cell life
The synergism of long nutrient, can improve the repairing quality of defective tissue.And when preparing fat stem cell, by II Collagen Type VI
Enzyme, papain composition epoxy glue protoenzyme digest so that fatty tissue digestion more thorough, it is thus achieved that fat stem cell
Purity is higher.
The foregoing is only embodiments of the invention, not thereby limit the scope of the claims of the present invention, every utilize this
Equivalent structure or equivalence flow process that bright description is made convert, or are directly or indirectly used in other relevant technology necks
Territory, is the most in like manner included in the scope of patent protection of the present invention.
Claims (10)
1. a fat stem cell filler, it is characterised in that include fat stem cell, become fiber stem cell, fibroblast,
Nutrient for cell growth, the density of described fat stem cell is 1 × 107-5×107Individual/ml, the density of one-tenth fiber stem cell are
0.5×107-1×107Individual/ml, fibroblastic density are 0.5 × 107-1×107Individual/ml, nutrient for cell growth dense
Degree is 20-30mg/ml.
Fat stem cell filler the most according to claim 1, it is characterised in that the density of described fat stem cell is 3
×107Individual/ml, the density of one-tenth fiber stem cell are 0.8 × 107Individual/ml, fibroblastic density are 0.8 × 107Individual/ml,
The concentration of nutrient for cell growth is 25mg/ml.
Fat stem cell filler the most according to claim 1, it is characterised in that the density of described fat stem cell is 2
×107Individual/ml, the density of one-tenth fiber stem cell are 0.6 × 107Individual/ml, fibroblastic density are 0.6 × 107Individual/ml,
The concentration of nutrient for cell growth is 25mg/ml.
4. according to the fat stem cell filler described in Claims 2 or 3 any one, it is characterised in that described fat is dry thin
Born of the same parents are the third generation to the fat stem cell in the 5th generation.
5. the preparation method of a fat stem cell filler, it is characterised in that comprise the following steps:
Nutrient for cell growth is completely dissolved in water, adds fat stem cell, become fiber stem cell, fibroblast, weight
Outstanding each cell, prepares fat stem cell filler；
Wherein, the density of described fat stem cell is 1 × 107-5×107Individual/ml, the density becoming fiber stem cell is 0.5 ×
107-1×107Individual/ml, fibroblastic density are 0.5 × 107-1×107Individual/ml, the concentration of nutrient for cell growth are
20-30mg/ml。
The preparation method of fat stem cell filler the most according to claim 5, it is characterised in that described fat stem cell
Density be 3 × 107Individual/ml, the density of one-tenth fiber stem cell are 0.8 × 107Individual/ml, fibroblastic density is 0.8 ×
107Individual/ml, the concentration of nutrient for cell growth are 25mg/ml.
The preparation method of fat stem cell filler the most according to claim 5, it is characterised in that described fat stem cell
Density be 2 × 107Individual/ml, the density of one-tenth fiber stem cell are 0.6 × 107Individual/ml, fibroblastic density is 0.6 ×
107Individual/ml, the concentration of nutrient for cell growth are 25mg/ml.
8. according to the preparation method of the fat stem cell filler described in claim 6 or 7 any one, it is characterised in that institute
Stating fat stem cell is the third generation to the fat stem cell in the 5th generation.
The preparation method of fat stem cell filler the most according to claim 8, it is characterised in that described fat stem cell
Obtain in the way of using mixing collagenase digesting.
The preparation method of fat stem cell filler the most according to claim 9, it is characterised in that described mixing collagen
Enzyme is made up of II Collagenase Type, papain, and concentration during its digestion fatty tissue is 0.1%(g/ml), wherein, epoxy glue
Protoenzyme 1%(g/ml) collocation method of mother solution is addition 0.5g II Collagenase Type, 0.5g Fructus Chaenomelis egg in 100ml D-Hank's solution
White enzyme.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610831877.4A CN106267353B (en) | 2016-09-19 | 2016-09-19 | Adipose-derived stem cell filler and preparation method thereof |
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| CN201610831877.4A CN106267353B (en) | 2016-09-19 | 2016-09-19 | Adipose-derived stem cell filler and preparation method thereof |
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| CN106267353A true CN106267353A (en) | 2017-01-04 |
| CN106267353B CN106267353B (en) | 2019-12-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115887773A (en) * | 2022-11-22 | 2023-04-04 | 杭州美约健康管理有限公司 | Autologous fibroblast regeneration collagen scaffold and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548988A (en) * | 2009-02-25 | 2009-10-07 | 北京赛尔泰和生物医药科技有限公司 | Injection for treating skin defects and preparation method thereof |
| CN105030645A (en) * | 2015-09-14 | 2015-11-11 | 广州赛莱拉干细胞科技股份有限公司 | Composition and preparing method and beauty preparation thereof |
| CN105748519A (en) * | 2016-04-06 | 2016-07-13 | 杭州元研细胞生物科技有限公司 | Preparation method of adipose mesenchymal stem cell preparation for treating osteoarthritis |
-
2016
- 2016-09-19 CN CN201610831877.4A patent/CN106267353B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548988A (en) * | 2009-02-25 | 2009-10-07 | 北京赛尔泰和生物医药科技有限公司 | Injection for treating skin defects and preparation method thereof |
| CN105030645A (en) * | 2015-09-14 | 2015-11-11 | 广州赛莱拉干细胞科技股份有限公司 | Composition and preparing method and beauty preparation thereof |
| CN105748519A (en) * | 2016-04-06 | 2016-07-13 | 杭州元研细胞生物科技有限公司 | Preparation method of adipose mesenchymal stem cell preparation for treating osteoarthritis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115887773A (en) * | 2022-11-22 | 2023-04-04 | 杭州美约健康管理有限公司 | Autologous fibroblast regeneration collagen scaffold and preparation method thereof |
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