CN110327244A - Reagent for skin care combines - Google Patents

Abstract

The present invention provides a kind of reagent combination for skin care, and the reagent combination includes the first reagent, the second reagent and third reagent, wherein first reagent splits yeast tunning lysis object comprising carnosine, animal umbilical cord extract, golden button flower extract and two；Second reagent includes tetrahydro methylpyrimidine carboxylic acid and ceramide；The third reagent includes macromolecular Sodium Hyaluronate, low-numerator sodium hyaluronate, hydrolysis Sodium Hyaluronate, glycine betaine and ethoxy urea.Reagent combination of the present invention for skin care can reduce wrinkle, and have good moisture retention and stability, can be good at improving skin condition.

Description

Reagent for skin care combines

Technical field

The present invention relates to skin care fields, and in particular to a kind of reagent combination and its application for skin care.

Background technique

With the improvement of people ' s living standards and the development of skin care item production technology, people to the pursuit of skin beauty increasingly It is easy, skin care is no longer the luxury of a few peoples, but has become the necessity of people's daily life.Meanwhile skin care industry also accumulates Educate a huge industry.Investment of the China every year in terms of " skin care " is huge.It will be appreciated, however, that in information overflow Today, for common people, due to lacking the knowledge of profession, either in terms of selecting skin care item, or in understanding skin care side All there are serious mistaken ideas in face.It is deteriorated however as environmental quality, the increase of operating pressure and some consumers or life Hormone keeps the tolerance of the external boundary's stimulation of people more next in the abuse of skin surface caused by fast efficacy is pursued in production person's transition Poorer, skin water shortage, skin ageing phenomenon are more and more frequent.

The main feature of skin aging is the increase of face wrinkles, this be both human gene pre-selection arrange program and There are the factor of environment, for example facial skin is constantly by compressing (as during sleep), muscle caused by regular countenance Contractile motion etc..From physiologically saying, the aging of skin shows the reduction of collagen and elastin laminin, skin oil and fat Random arrangement.

Aging is also known as aging, is the only stage which must be passed by vital movement process, and skin aging shows during body aging It is the most obvious.Skin aging not only will appear wrinkle, relaxation and pigmentation, at the same there is also epidermis metabolism reduce, Epidermis reparation is damaged ability and barrier protection reduced capability.So anti-aging skin care product has become the majority of consumers' urgent need One of category.

The mainstream skin care consciousness of people is also maintained at most basic moisturizing moisturizing at present, while moisturizing moisturizing is also skin care item Carry out earliest in research and is easier the effect of realizing.Demand with people to skin-care effect is higher and higher how Accomplish the safety that not only can guarantee product, but also there are authentic and valid anti-aging effects, this is also exactly desired by consumer 's.

Patent CN105030610A discloses a kind of anti-apolexis composition, comprising basic components and is scattered in the basis The anti-aging factor in component, the anti-aging factor are selected from soybean germ extract, wheat malt germ extract, safflower plumule and mention Take object, at least three kinds in maize germ extract.Patent CN105030610A is by by soybean germ extract, wheat embryo Extract, safflower germ extracts, at least three kinds in maize germ extract as the anti-aging factors be applied to anti-aging group It closes in object, three kinds of substances generate synergistic effect on antisenility between each other, can cooperate with and promote collagen synthesis, quickly smooth skin Microgroove.But skin is limited to its assimilation effect, and anti-ageing the effect is unsatisfactory.

Therefore, it is necessary to develop the good skin-protection product of a kind of anti-ageing, reparation, moisturizing, stability, microgroove can not only be desalinated And it can preferably improve skin condition from internal repair skin.

Summary of the invention

In view of this, the skin-protection product good the purpose of the present invention is to provide a kind of anti-ageing, reparation, moisturizing, stability, Microgroove can not only be desalinated and can preferably improve skin condition from internal repair skin.

For this purpose, the present invention provides following technical schemes.

In a first aspect, the reagent combination that the present invention provides a kind of for skin care, the reagent combination comprising the first reagent, Second reagent and third reagent, wherein

First reagent splits yeast tunning lysis comprising carnosine, animal umbilical cord extract, golden button flower extract and two Object；

Second reagent includes tetrahydro methylpyrimidine carboxylic acid and ceramide；

The third reagent include macromolecular Sodium Hyaluronate, low-numerator sodium hyaluronate, hydrolysis Sodium Hyaluronate, glycine betaine and Ethoxy urea.

Wherein, described two split yeast tunning lysis object can be commercially available, such as the present invention be purchased from CLR Chemisches Laboratorium Dr.Kurt Richter GmbH, lot number 3191570.

In a preferred embodiment, according to parts by weight, first reagent includes:

0.1-2 parts of carnosines, for example, the parts by weight of the carnosine can for 0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8, 0.9,1,1.1,1.3,1.5,1.7,1.8,1.9 or 2；

0.1-15 parts of animal umbilical cord extracts, for example, the parts by weight of the animal umbilical cord extract can for 0.1,0.5,1, 1.5,2,2.5,3,3.5,4,4.5,5,5.5,6,6.5,7,7.5,8,8.5,9,9.5,10,11,12,13,14 or 15；

0.1-10 parts of golden button flower extracts, for example, the parts by weight of the gold button flower extract can for 0.1,0.5,1, 1.5,2,2.5,3,3.5,4,4.5,5,5.5,6,6.5,7,7.5,8,8.5,9,9.5 or 10；

Yeast tunning lysis object is split with 0.1-60 part two, for example, described two split the parts by weight of yeast tunning lysis object Number can be 0.1,1,2,3,4,5,7,10,12,15,17,20,22,25,30,32,35,40,45,50,52,55,57 or 60.

In preferred embodiment, the animal can be pig, ox or sheep.

In a preferred embodiment, according to parts by weight, second reagent includes:

0.1-4 parts of tetrahydro methylpyrimidine carboxylic acids, for example, the parts by weight of the tetrahydro methylpyrimidine carboxylic acid can for 0.1,0.5, 1,1.5,2,2.5,3,3.5 or 4；

With 0.1-30 parts of ceramides, for example, the parts by weight of the ceramide can for 0.1,1,2,3,4,5,7,10, 12,15,17,20,22,25 or 30.

In a preferred embodiment, according to parts by weight, the third reagent includes:

0.05-1 parts of macromolecular Sodium Hyaluronates, for example, the parts by weight of the macromolecular Sodium Hyaluronate can for 0.05, 0.06,0.07,0.08,0.09 or 1；

0.05-1 parts of low-numerator sodium hyaluronates, for example, the parts by weight of the low-numerator sodium hyaluronate can for 0.05, 0.06,0.07,0.08,0.09 or 1；

0.05-1 parts of hydrolysis Sodium Hyaluronates, for example, the parts by weight of the hydrolysis Sodium Hyaluronate can for 0.05,0.06, 0.07,0.08,0.09 or 1；

0.1-15 parts of glycine betaines, for example, the parts by weight of the glycine betaine can be 0.1,1,2,3,4,5,7,10,12 or 15；

With 0.1-60 parts of ethoxy ureas, for example, the parts by weight of the ethoxy urea can for 0.1,1,2,3,4,5,7,10, 12,15,17,20,22,25,30,32,35,40,45,50,52,55,57 or 60.

In a preferred embodiment, the reagent combination also includes grease, emulsifier, preservative and water, wherein

The grease is selected from grape seed oil, corn oil, sesame oil, soybean oil, peanut oil, sunflower seed oil, synthesis saualane, gathers One of dimethyl siloxane, myristic acid, palmitinic acid, stearic acid, beeswax, paraffin and vaseline are a variety of；

The emulsifier is selected from PPG-5- ceteth -20, stereth -2, stereth -21, cetearyl Portugal Glucosides, ceteth -20, ceteareth -25, polyglycereol-3-methyl distearate, Natrulon H-10 are stearic Acid esters, Natrulon H-10 myristinate, -6 lauric acid of Natrulon H-10 isostearate, Natrulon H-10 oleate and polyglycereol One of ester is a variety of；

The preservative be selected from methyl hydroxybenzoate, Nipasol, Phenoxyethanol, glycerol caprylate, Sensiva SC50, benzoic acid, One of sodium benzoate, sorbic acid and potassium sorbate are a variety of.

Wherein, the synthesis saualane can be commercially available, such as the present invention is purchased from BASF China, batch Number be 180920A.

Second aspect, the reagent combination that the present invention provides a kind of for skin care, the reagent combination comprising the first reagent, Second reagent and third reagent, wherein according to parts by weight,

First reagent includes 0.1-4 parts of carnosines, 0.1-20 parts of animal umbilical cord extracts, 0.1-15 parts of golden button flower extracts Yeast tunning lysis object is split with 0.1-80 part two；

Second reagent includes 0.1-4 parts of tetrahydro methylpyrimidine carboxylic acids and 0.1-30 parts of ceramides；

The third reagent includes 0.05-1 parts of macromolecular Sodium Hyaluronates, 0.05-1 parts of low-numerator sodium hyaluronates, 0.05-1 Part hydrolysis Sodium Hyaluronate, 0.1-15 parts of glycine betaines and 0.1-60 parts of ethoxy ureas.

In a preferred embodiment, the reagent combination also includes grease, emulsifier, preservative and water, wherein

The grease is selected from grape seed oil, corn oil, sesame oil, soybean oil, peanut oil, sunflower seed oil, saualane, poly- diformazan One of radical siloxane, myristic acid, palmitinic acid, stearic acid, beeswax, paraffin and vaseline are a variety of；

The emulsifier is selected from PPG-5- ceteth -20, stereth -2, stereth -21, cetearyl Portugal Glucosides, ceteth -20, ceteareth -25, polyglycereol-3-methyl distearate, Natrulon H-10 are stearic Acid esters, Natrulon H-10 myristinate, -6 lauric acid of Natrulon H-10 isostearate, Natrulon H-10 oleate and polyglycereol One of ester is a variety of；

The preservative be selected from methyl hydroxybenzoate, Nipasol, Phenoxyethanol, glycerol caprylate, Sensiva SC50, benzoic acid, One of sodium benzoate, sorbic acid and potassium sorbate are a variety of.

The third aspect, the present invention provides a kind of preparation methods that the reagent for skin care combines, wherein the method packet Containing following steps:

S1. by macromolecular Sodium Hyaluronate, low-numerator sodium hyaluronate, hydrolysis Sodium Hyaluronate, glycine betaine, ethoxy urea and water It is added in water phase pot and is uniformly mixed, be heated to 75-85 DEG C of heat preservation 20-30min to being completely dissolved, obtain mixture A；

S2. emulsifier and grease are added to oily phase pot to be uniformly mixed, are heated to being completely melt, until 75-85 DEG C, keep the temperature 20- 30min obtains oily phase B；

S3. the oil phase B and mixture A is uniformly mixed in emulsion pot, at 70-80 DEG C of emulsifying 10-20min points Clock, and vacuum defoamation is carried out simultaneously, obtain mixture C；

S4. carnosine, animal umbilical cord extract, golden bachelor's button is added after the mixture C cold bath being cooled to 35-45 DEG C to extract Object, two split yeast tunning lysis object, tetrahydro methylpyrimidine carboxylic acid, ceramide and preservative, and 50-60r/min stirring is equal It is even, 33-38 DEG C of discharging.

Fourth aspect, provides a kind of skin-protection product, and the skin-protection product is combined comprising reagent of the present invention.

5th aspect, provides a kind of pharmaceutical composition, and described pharmaceutical composition includes safe and effective amount such as the present invention The reagent combination.

In addition, additionally providing the reagent combination of the present invention for skin care is preparing the purposes in skin-protection product.

Additionally, it is provided the purposes of the present invention for the reagent combination of skin care in preparation pharmaceutical composition.

In the present invention, animal umbilical cord extract the preparation method is as follows:

S1. the residual blood gone in animal umbilical cord is squeezed, animal umbilical cord is placed in the beaker of 5L, about 3L purified water, cleaning is added Animal umbilical cord at least cleans 3 times to blood stains are visible by naked eyes；

S2. animal umbilical cord is transferred in culture dish, animal umbilical cord is cut into 2-4 sections with scissors, with tweezers along animal umbilical cord Texture tears animal umbilical cord, extracts 3 blood vessels out；

S3. animal umbilical cord is placed in the beaker of 5L, be added about 3L purified water, cleaning animal umbilical cord to be visible by naked eyes blood stains, Without sticky tissue fluid, at least clean 3 times；

S4. purified water is discarded, 75% alcohol of about 1L is added and submerges animal umbilical cord tissue, stands 120min, discards alcohol；

S5. about 3L purified water is added, cleans animal umbilical cord, discards purified water, at least cleans 3 times；

S6. animal umbilical cord tissue is shredded with scissors, under condition of ice bath, is homogenized with refiner, 10000rpm, 10min；

S7. animal umbilical cord tissue is weighed, with 50g (animal umbilical cord tissue): the ratio of 10L (phosphate buffer) extracts animal navel Band tissue, blender stir 30min with 200rpm, ice bath；

S8. leaching liquor is fitted into centrifuge tube, with 12000rpm, 4 DEG C, is centrifuged 15min, collects supernatant；

S9. ultrafiltration

S9.1. ultrafiltration cleans

S9.1.1. system is installed: being put gasket well, 0.5 ㎡ film packet of 30KD is put in Pellicon fixture, by screw tightening；

S9.1.2. it rinses: sterile pure water being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rpm or so, carries out reflux end and rinses, flush volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.2bar, rush through end It washes, flush volume is 10L-20L/ ㎡；

S9.1.3. ultrafiltration cleans: being packed into feed liquid in head tank, return pipe is put into head tank, be put into collection through pipe and hold In device, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.9bar, start timing when permeate enters container, divide 1-2 times Filter wash is carried out, until the volume of the concentrated liquid reaches 10L or so, ultrafiltration is terminated, keeps sample；

S9.1.4. it rinses: sterile pure water being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rpm or so, carries out reflux end and rinses, flush volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.2bar, rush through end It washes, flush volume is 10L-20L/ ㎡；

S9.1.5. it cleans: 0.5M NaOH being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rpm or so, carries out the cleaning of reflux end, and cleaning volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.2bar, carries out clear through end It washes, cleaning volume is 10L-20L/ ㎡；

S9.1.6. it carries out reflux end to rinse, pH detection is carried out with liquid of the PH test paper to the outflow of reflux end, until pH reaches neutrality i.e. Flushing can be terminated；Return pipe is then put back into purge tank, is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane Pressure reaches 0.2bar, rinse through end, pH detection is carried out to the liquid through end outflow with PH test paper, until pH reaches neutral Flushing can be terminated；

S9.1.7. it unloads film packet: film packet and gasket is taken out from fixture, be soaked in 1% sodium hydrogensulfite；

S9.2. it is concentrated by ultrafiltration

S9.2.1. system is installed: 0.5 ㎡ film packet of 3KD is put in parallel in Pellicon fixture, by screw tightening；

S9.2.2. it rinses: sterile pure water being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rmp or so, carries out reflux end and rinses, flush volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.9bar, rush through end It washes, flush volume is 10L-20L/ ㎡；

S9.2.3. it is concentrated by ultrafiltration: pouring into removal of impurities permeate in head tank, return pipe is put into head tank, be in through pipe Discharge state, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.9bar, starts to collect when permeate outflow, until concentrate Volume and material liquid volume reach 1:4 or so, terminate ultrafiltration, keep sample；

S9.2.4. it rinses: sterile pure water being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rmp or so, carries out reflux end and rinses, flush volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.9bar, rush through end It washes, flush volume is 10L-20L/ ㎡；

S9.2.5. it cleans: 0.1M NaOH being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve is complete It opens, adjusting revolution speed is 17rpm or so, carries out the cleaning of reflux end, and cleaning volume is 10L-20L/ ㎡；Then return pipe is put back to Purge tank is in discharge state through pipe, adjusts revolution speed and return valve to permeable membrane pressure reaches 0.9bar, carries out clear through end It washes, cleaning volume is 10L-20L/ ㎡；

S9.2.6. it rinses: ultrapure water being added in purge tank, will transmit through pipe and return pipe is in discharge state, return valve standard-sized sheet, Adjusting revolution speed is 17rpm or so, carries out reflux end and rinses, and carries out pH detection with liquid of the pH test paper to the outflow of reflux end, until PH, which reaches neutrality, can terminate flushing；Return pipe is then put back into purge tank, is in discharge state through pipe, adjust revolution speed and Return valve to permeable membrane pressure reaches 0.9bar, rinse through end, carries out pH detection to the liquid through end outflow with pH test paper, Flushing can be terminated by reaching neutrality to pH；

S9.2.7. it unloads film packet: film packet is taken out from fixture, be soaked in 1% sodium hydrogensulfite.

S10. filling and packaging finishes, and 60Co-γ rays 25KGy irradiation is carried out in 12 hours.

Referring now to the prior art, the beneficial effects of the present invention are be:

The present invention is produced by the way that the carnosine of certain content, animal umbilical cord extract, golden button flower extract and two are split yeast fermentation Object lysis object is applied in the reagent combination for skin care as the anti-aging factor, and it is anti-that the substance of four kinds of certain contents generates collaboration Aging effect has good quenching effect, while the still chelating agent of heavy metal to active oxygen radical, is able to suppress albumen Matter glycosylation, protein glycosylation are that intracorporal glucose molecule or other carbohydrate molecule mistakes paste protein On, cause protein denaturation, this adverse reaction, to aging important role, while can prevent glue as oxidation reaction Former protide macromolecules cross-linking, the synthesis of stimulation collagen achieve the purpose that desalinate wrinkle.

The present invention passes through using the tetrahydro methylpyrimidine carboxylic acid of certain content and ceramide as skin repair factor application In the reagent combination for skin care, the substance of two kinds of certain contents generates collaboration skin repair effect, can repair ultraviolet light Caused by skin damage, accelerate cell repair, enhance cutaneous immunisation, while ceramide can increase moisture content of skin, restore Skin elasticity and anti-aging, to enhance the natural barrier function of skin.

The present invention by by the macromolecular Sodium Hyaluronate of certain content, low-numerator sodium hyaluronate, hydrolysis hyaluronic acid Sodium, glycine betaine and ethoxy urea are applied in the reagent combination for skin care as moisturizing factor, the substance of five kinds of certain contents Collaboration moisture-keeping function is generated, the macromolecular Sodium Hyaluronate used can form one layer of special protective film in cell surface, can With Bound Water Moleculess, it can also play the role of in dehydration instead of moisture in cell, to protect Skin Cell that will not be damaged； The low-numerator sodium hyaluronate used plays the role of moisturizing, and the molecular weight of the hydrolysis Sodium Hyaluronate used is minimum, can be by skin It absorbs.

Provided by the present invention for the reagent combination of skin care, it is able to maintain in low temperature (- 18 DEG C) to high temperature (48 DEG C) fabulous Stability.

In another aspect, the reagent set of skin care is used for for the type of component in the reagent combination of skin care and adjustment by screening The parts by weight of component in conjunction, the present invention can be good at reducing wrinkle for the reagent combination of skin care, and has and protect well Moist and stability.

Detailed description of the invention

Fig. 1 is the anti-aging test result that the reagent for skin care prepared by embodiment 1 combines；

Fig. 2 is the anti-aging test result that the reagent for skin care prepared by embodiment 2 combines；

Fig. 3 is the anti-aging test result that the reagent for skin care prepared by embodiment 3 combines；

Fig. 4 is the anti-aging test result that the reagent for skin care prepared by comparative example 1 combines；

Fig. 5 is the anti-aging test result that the reagent for skin care prepared by comparative example 9 combines.

Specific embodiment

Below in conjunction with specific embodiment and embodiment, it is specifically described the present invention, advantages of the present invention and various effects It thus more will clearly present.It will be understood by those skilled in the art that these specific embodiments and embodiment are for illustrating The present invention is not intended to limit the present invention.

Throughout the specification, unless otherwise specified, terms used herein are interpreted as usual in this field Used meaning.As a result, unless otherwise defined, all technical and scientific terms used herein has leads with belonging to the present invention The identical meaning of the general understanding of field technique personnel.Contradiction if it exists, this specification are preferential.

Emulsifier

In the present invention, emulsifier is to refer to improve the various surface tension constituted between phase in emulsion, is allowed to be formed The substance of even stable dispersion or emulsion.

For the type of emulsifier, it is poly- that the present invention is selected from PPG-5- ceteth -20, stereth -2, stearyl alcohol Ether -21, cetearyl glucoside, ceteth -20, ceteareth -25, -3 methyl distearyl of polyglycereol Acid esters, Natrulon H-10 stearate, Natrulon H-10 myristinate, Natrulon H-10 isostearate, Natrulon H-10 oleic acid One of -6 laurate of ester and polyglycereol is a variety of.

For the dosage of emulsifier, those skilled in the art can select according to the type of emulsifier, for example, making The parts by weight of 2-5 can be used with PPG-5- ceteth -20.

Grease

In the present invention, the grease is selected from grape seed oil, corn oil, sesame oil, soybean oil, peanut oil, sunflower seed oil, angle One of shark alkane, dimethyl silicone polymer, myristic acid, palmitinic acid, stearic acid, beeswax, paraffin and vaseline are a variety of.

For the dosage of grease, those skilled in the art can select according to the type of grease, for example, using conjunction At the parts by weight that can use such as 0.5-1 when saualane.

Preservative

In the present invention, for the purpose of preservative refers to the growth and breeding to inhibit microorganism in the reagent combination for skin care And the substance being added in the reagent combination for skin care, for extending the holding time of the reagent combination for skin care.

There is different mode classifications for the preservative in the reagent combination of skin care.It is commonly used in the reagent combination of skin care In preservative have methyl hydroxybenzoate, Nipasol, Phenoxyethanol, glycerol caprylate, Sensiva SC50, benzoic acid, benzoic acid Sodium, sorbic acid and potassium sorbate.

For the dosage of preservative, those skilled in the art can select according to the type of preservative, for example, The parts by weight that such as 0.1-1 can be used when using Phenoxyethanol, can be using for example when using methyl hydroxybenzoate The parts by weight of 0.1-0.5.

Skin-protection product

Unless specifically indicated, the term " first " in the present invention, " second ", " third " are only used for distinguishing multiple similar elements, Any difference without being intended to mean that importance between element or order etc..

In a preferred embodiment, reagent of the invention combination is contained in skin-protection product.

Skin-protection product of the invention may include container, operation instructions, using auxiliary agent, the 4th reagent, the 5th as needed Reagent and/or for being tested for the property required other structures and/or reagent.

Include but is not limited to for being tested for the property required other structures in skin-protection product of the invention, for sampling Structure, for carrying out contrast structure and/or for Observe and measure process or the structure of result.

Being used to be tested for the property other required reagents in the present invention includes but is not limited to culture medium, detergent, colour developing Agent and/or terminator, and except thickener of the invention, emulsifier, surfactant, skin conditioning agent, grease, fragrance and prevent Other thickeners, emulsifier, surfactant, skin conditioning agent, grease, fragrance and preservative outside rotten agent.

Certainly, it will be understood by those skilled in the art that in addition to the foregoing, may be used also in reagent combination of the present invention To include other accessory ingredients, such as free radical scavenger, antioxidant, film forming agent, stabilizer.These accessory ingredients are according to it Dissolubility in the first reagent, the second reagent and third reagent and be included in the first reagent, the second reagent and/or third examination In agent.

In the present invention, pharmaceutical composition includes reagent of the present invention combination and pharmaceutically acceptable auxiliary material.

In the present invention, safe and effective amount refers to that a certain amount of compound or composition, the amount are enough significantly to induce product The beneficial effect of pole, preferably positive skin histology appearance or feeling beneficial effect, include herein in form independent or combined Disclosed beneficial effect, but the amount and sufficiently low to avoid serious side effect, i.e., the model rationally judged in technical staff The interior reasonable effective hazard ratio of offer is provided.

The present invention is described in more detail by the following examples, but the present invention is not limited to these Examples.

Embodiment 1

The present embodiment provides a kind of reagent combinations for skin care, and the reagent combination includes the first reagent, the second reagent, third Reagent, grease, emulsifier, preservative and water, wherein according to parts by weight,

First reagent includes: 0.1 part of carnosine, 0.1 part of pig umbilical cord extract, 0.1 part of golden button flower extract and 0.1 part two Split yeast tunning lysis object；

Second reagent includes: 0.1 part of tetrahydro methylpyrimidine carboxylic acid and 0.1 part of ceramide；

The third reagent includes: 0.05 part of macromolecular Sodium Hyaluronate, 0.05 part of low-numerator sodium hyaluronate, 0.05 part of hydrolysis Sodium Hyaluronate, 0.1 part of glycine betaine and 0.1 part of ethoxy urea；

The grease includes 0.5 part of synthesis saualane；

The emulsifier includes 2 parts of PPG-5- ceteths -20；

The preservative includes 0.1 part of Phenoxyethanol and 0.1 part of methyl hydroxybenzoate.

The present embodiment for skin care reagent combine the preparation method is as follows:

S1. by 0.05 part of macromolecular Sodium Hyaluronate, 0.05 part of low-numerator sodium hyaluronate, 0.05 part of hydrolysis Sodium Hyaluronate, 0.1 part of glycine betaine, 0.1 part of ethoxy urea and water are added in water phase pot and are uniformly mixed, and are heated to 75 DEG C of heat preservation 20min to complete Dissolution, obtains mixture A；

S2. 2 parts of PPG-5- ceteth -20 and 0.5 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 20min, obtains oily phase B to 75 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 70 DEG C emulsifying 10min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 0.1 part of carnosine, 0.1 part of pig umbilical cord extract, 0.1 part of gold are added after the mixture C cold bath being cooled to 35 DEG C Button flower extract, 0.1 part two split yeast tunning lysis object, 0.1 part of tetrahydro methylpyrimidine carboxylic acid, 0.1 part of ceramide, 0.1 part of Phenoxyethanol and 0.1 part of methyl hydroxybenzoate, 50r/min are stirred evenly, 33 DEG C of dischargings.

Embodiment 2

The present embodiment provides a kind of reagent combinations for skin care, and the reagent combination includes the first reagent, the second reagent, third Reagent, grease, emulsifier, preservative and water, wherein according to parts by weight,

First reagent includes: 4 parts of carnosines, 20 parts of pig umbilical cord extracts, 15 parts of golden button flower extracts and 80 part two split ferment Female tunning lysis object；

Second reagent includes: 4 parts of tetrahydro methylpyrimidine carboxylic acids and 30 parts of ceramides；

The third reagent includes: 1 part of macromolecular Sodium Hyaluronate, 1 part of low-numerator sodium hyaluronate, 1 part of hydrolysis hyaluronic acid Sodium, 15 parts of glycine betaines and 60 parts of ethoxy ureas；

The grease includes 1 part of synthesis saualane；

The emulsifier includes 5 parts of PPG-5- ceteths -20；

The preservative includes 1 part of Phenoxyethanol and 0.5 part of methyl hydroxybenzoate.

The present embodiment for skin care reagent combine the preparation method is as follows:

S1. by 1 part of macromolecular Sodium Hyaluronate, 1 part of low-numerator sodium hyaluronate, 1 portion of hydrolysis Sodium Hyaluronate, 15 portions of beets Alkali, 60 ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 85 DEG C of heat preservation 30min to being completely dissolved, must mix Object A；

S2. 5 parts of PPG-5- ceteth -20 and 1 part synthesis saualanes are added to oily phase pot to be uniformly mixed, are heated to completely 85 DEG C are melted to, 30min is kept the temperature, obtains oily phase B；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 80 DEG C emulsifying 20min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 4 parts of carnosines, 20 parts of pig umbilical cord extracts, 15 parts of golden buttons are added after the mixture C cold bath being cooled to 45 DEG C Flower extract, 80 part two split yeast tunning lysis object, 4 parts of tetrahydro methylpyrimidine carboxylic acids, 30 parts of ceramides, 1 part of benzene oxygen Ethyl alcohol and 0.5 part of methyl hydroxybenzoate, 60r/min are stirred evenly, 38 DEG C of dischargings.

Embodiment 3

The present embodiment provides a kind of reagent combinations for skin care, and the reagent combination includes the first reagent, the second reagent, third Reagent, grease, emulsifier, preservative and water, wherein according to parts by weight,

First reagent includes: 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden button flower extracts and 50 part two split yeast Tunning lysis object；

Second reagent includes: 1 part of tetrahydro methylpyrimidine carboxylic acid and 20 parts of ceramides；

The third reagent includes: 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis are saturating Bright matter acid sodium, 10 parts of glycine betaines and 40 parts of ethoxy ureas；

The grease includes 0.8 part of synthesis saualane；

The emulsifier includes 3 parts of PPG-5- ceteths -20；

The preservative includes 0.5 part of Phenoxyethanol and 0.3 part of methyl hydroxybenzoate.

The present embodiment for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 1

Beneficial effect in order to further illustrate the present invention provides comparative example 1 and compares with embodiment 3, the difference of the comparative example 1 It is: synthesis saualane of the invention is replaced with into Permethyl 99A.

Comparative example 1 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part Permethyl 99As are added to oily phase pot to be uniformly mixed, are heated to completely 80 DEG C are melted to, 25min is kept the temperature, obtains oily phase B；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 2

Beneficial effect in order to further illustrate the present invention provides comparative example 2 and compares with embodiment 3, the difference of the comparative example 2 It is: PPG-5- ceteth -20 of the invention is replaced with into PPG-13- decyl tetradecyl alcohol polyethers -24.

Comparative example 2 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-13- decyl tetradecyl alcohol polyethers -24 and 0.8 part of synthesis saualane are added to oily phase pot and are uniformly mixed, added Heat keeps the temperature 25min, obtains oily phase B to being completely melt to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 3

Beneficial effect in order to further illustrate the present invention provides comparative example 3 and compares with embodiment 3, the difference of the comparative example 3 It is: preservative of the invention is replaced with into Phenoxyethanol.

Comparative example 3 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides and 0.3 part of hydroxyl Benzene methyl, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 4

Beneficial effect in order to further illustrate the present invention provides comparative example 4 and compares with embodiment 3, the difference of the comparative example 4 It is: does not add golden button flower extract.

Comparative example 4 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. after the mixture C cold bath being cooled to 40 DEG C add 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 50 part two split ferment Female tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of Phenoxyethanol and 0.3 part of oxybenzene first Ester, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 5

Beneficial effect in order to further illustrate the present invention provides comparative example 5 and compares with embodiment 3, the difference of the comparative example 5 It is: does not add ceramide.

Comparative example 5 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 0.5 part of Phenoxyethanol and 0.3 part Methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 6

Beneficial effect in order to further illustrate the present invention provides comparative example 6 and compares with embodiment 3, the difference of the comparative example 6 It is: does not add macromolecular Sodium Hyaluronate.

Comparative example 6 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts of glycine betaines, 40 parts of ethoxy ureas and Water is added in water phase pot and is uniformly mixed, and is heated to 80 DEG C of heat preservation 25min to being completely dissolved, obtains mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 7

Beneficial effect in order to further illustrate the present invention provides comparative example 7 and compares with embodiment 3, the difference of the comparative example 7 It is: does not add low-numerator sodium hyaluronate.

Comparative example 7 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts of glycine betaines, 40 parts of ethoxy ureas and Water is added in water phase pot and is uniformly mixed, and is heated to 80 DEG C of heat preservation 25min to being completely dissolved, obtains mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 8

Beneficial effect in order to further illustrate the present invention provides comparative example 8 and compares with embodiment 3, the difference of the comparative example 8 It is: does not add hydrolysis Sodium Hyaluronate.

Comparative example 8 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 10 parts of glycine betaines, 40 parts of ethoxy ureas It is added in water phase pot and is uniformly mixed with water, be heated to 80 DEG C of heat preservation 25min to being completely dissolved, obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 0.5 part of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 9

Beneficial effect in order to further illustrate the present invention provides comparative example 9 and compares with embodiment 3, the difference of the comparative example 9 It is: the parts by weight of Phenoxyethanol is adjusted to 1.2.

Comparative example 9 for skin care reagent combine the preparation method is as follows:

S1. by 0.1 part of macromolecular Sodium Hyaluronate, 0.1 part of low-numerator sodium hyaluronate, 0.1 part of hydrolysis Sodium Hyaluronate, 10 parts Glycine betaine, 40 parts of ethoxy ureas and water are added in water phase pot and are uniformly mixed, and are heated to 80 DEG C of heat preservation 25min to being completely dissolved, Obtain mixture A；

S2. 3 parts of PPG-5- ceteth -20 and 0.8 part synthesis saualanes are added to oily phase pot to be uniformly mixed, have been heated to Running down keeps the temperature 25min, obtains oily phase B to 80 DEG C；

S3. by the oil phase B and mixture A in emulsion pot be uniformly mixed, 75 DEG C emulsifying 15min minutes, and Vacuum defoamation is carried out simultaneously, obtains mixture C；

S4. 2 parts of carnosines, 10 parts of pig umbilical cord extracts, 5 parts of golden buttons are added after the mixture C cold bath being cooled to 40 DEG C Flower extract, 50 part two split yeast tunning lysis object, 1 part of tetrahydro methylpyrimidine carboxylic acid, 20 parts of ceramides, 1.2 parts of benzene Oxyethanol and 0.3 part of methyl hydroxybenzoate, 55r/min are stirred evenly, 35 DEG C of dischargings.

Comparative example 10

Beneficial effect in order to further illustrate the present invention provides a kind of anti-apolexis composition, which is to use Prepared by the method in patent CN105030610A.

Test the detection of 1:pH value

The pH value of 1-3 of the embodiment of the present invention sample prepared detects in Shaanxi quality of medical care supervision and inspection institute, detection knot Fruit is as shown in table 1 below.

Table 1pH value testing result

Given the test agent	PH value
		Embodiment 1	5.81
Embodiment 2	5.81
		Embodiment 3	5.82

It can be seen from the above result that:

Human facial skin is in weak acid environment under normal circumstances, and pH value is 5.5 or so, if skin-protection product pH value is excessively high, no But can cause skin: stimulation, rubescent, the uncomfortable reaction such as itch can also destroy skin acid environment, cause the various discomforts of skin.

Reagent combination provided by the present invention for skin care is weakly acidic, and pH stablizes in 5.5-6.5 or so, close to skin Normal pH, utmostly affine skin is non-stimulated to skin, is able to maintain skin acid-base balance, is suitble to any skin quality, and can grow Phase uses.

Experiment 2: stability test

Given the test agent: the sample that 1-3 of the embodiment of the present invention, comparative example 1-10 are prepared.

Experimental method:

By each given the test agent respectively under -18 DEG C, room temperature, 48 DEG C and four test conditions of -18-48 DEG C of alternatings test 15d (day), 30d (day), 40d (day), observing each given the test agent, whether there is or not significant changes, and unchanged to be qualified, it is unqualified for changing, test knot Fruit is as shown in table 2 below.

2 stability test result of table

It can be seen from the above result that:

The present invention combines respectively under -18 DEG C, room temperature, 48 DEG C and four test conditions of -18-48 DEG C of alternatings for the reagent of skin care It after testing 15d (day), 30d (day), 40d (day), has no significant change, test result is qualification, i.e., the present invention is for protecting The reagent combination of skin has good cold resistance and heat resistance, while the use that can satisfy under the conditions of temperature extreme variation is wanted It asks.And the reagent combination for skin care of inventive formulation system is not used, cold resistance under long-time preservation condition compared with Difference, while being unable to satisfy the requirement under the conditions of temperature extreme variation.

Experiment 3: anti-aging test

Given the test agent: the sample that embodiment 1-3, comparative example 1 and comparative example 9 are prepared

Experimental method:

Wrinkle index Value Data of the present invention is opened up up to Science and Technology Ltd. by Beijing ancient cooking vessel victory and buys the survey of VISIA skin of face detector ?.5 25-55 years old healthy volunteers are tested, the tested portion of volunteer before first record is combined using reagent of the present invention for skin care The wrinkle of position starts later respectively in the face continuous use embodiment 1-3 of volunteer, comparative example 1 and comparative example 9 for protecting The reagent of skin combines, and smears position as full face smearing, once in the morning and once at night, continuous smearing tests volunteer after 4 weeks, 4 weeks Facial wrinkles with it is on probation before data compare, choose five groups of numbers for wherein trying out embodiment 1-3, comparative example 1 and comparative example 9 According to data are shown in that Fig. 1-5, horizontal axis indicate that testing time, the longitudinal axis indicate that wrinkle percentage, wrinkle percentage are smaller, it was demonstrated that wrinkle is got over It is few.

By Fig. 1-3 it is found that the face wrinkles of the volunteer combined using the reagent that the present invention is used for skin care are significantly reduced, lead to It crosses and advanced optimizes the present invention for the formula system that the reagent of skin care combines, reagent set conjunction of the present invention for skin care achieves Better anti-aging effects.

By Fig. 4-5 it is found that by replacement the present invention for skin care reagent combine in component and adjust the present invention be used for In the reagent combination of skin care after the parts by weight of component, face wrinkles' reduction of volunteer is unobvious, and anti-aging effects are unobvious.

Experiment 4: moisture retention test

Given the test agent: the sample that embodiment 1-3, comparative example 1, comparative example 9 are prepared

Experimental method:

Transepidermal water loss rate (TEWL) and skin moisture content data by German CK company skin quality diagnostician Derma- ExpertMC 960 is measured, and TWEL numerical value shows the percentage of water loss of a period of time inner skin epidermis, shows skin barrier function indirectly It can be improved, if TEWL value declines, be illustrated in a period of time, moisture of skin windage decline, skin barrier function obtains Improve, to illustrate that reagent combination of the present invention for skin care has maintenance effect to skin.Data in table are each test point Five groups of data are surveyed, then averaged.Test result is as follows shown in table 3.

3 moisture retention test result of table

It can be seen from the above result that:

After being combined using reagent of the present invention for skin care, TEWL value is reduced, and transepidermal water loss rate reduces, moisture of skin windage Decline, skin barrier function are improved, to illustrate that reagent combination of the present invention for skin care has maintenance effect to skin, together When using the present invention for the reagent combination of skin care after, skin moisture content also obviously increases, and illustrates the present invention for skin care The moisture retention of reagent combination is preferable.

It is used for the reagent set of skin care with the adjustment present invention for the component in the reagent combination of skin care by the replacement present invention In conjunction after the parts by weight of component, transepidermal water loss rate numerical value is essentially unchanged, and surface skin barrier function is not improved, together When skin moisture content reduced, illustrate be not used inventive formulation system for skin care reagent combination moisture retention compared with Difference.

It should be understood that the present invention disclosed is not limited only to specific method, scheme and the substance of description, because these It is alterable.It will also be understood that purpose of the terminology used here just for the sake of the specific embodiment scheme of description, rather than It is intended to limit the scope of the invention, the scope of the present invention is limited solely by the attached claims.

Those skilled in the art, which will also be appreciated that or be able to confirm that, uses no more than routine experiment, institute herein The many equivalents for the specific embodiment of the invention stated.These equivalents are also contained in the attached claims.

Claims (10)

1. a kind of reagent for skin care combines, the reagent combination includes the first reagent, the second reagent and the

Three reagents, wherein

First reagent splits yeast tunning lysis comprising carnosine, animal umbilical cord extract, golden button flower extract and two Object；

Second reagent includes tetrahydro methylpyrimidine carboxylic acid and ceramide；

The third reagent include macromolecular Sodium Hyaluronate, low-numerator sodium hyaluronate, hydrolysis Sodium Hyaluronate, glycine betaine and Ethoxy urea.

2. reagent combination according to claim 1, wherein according to parts by weight,

First reagent includes 0.1-2 parts of carnosines, 0.1-15 parts of animal umbilical cord extracts, 0.1-10 parts of golden button flower extracts Yeast tunning lysis object is split with 0.1-60 part two.

3. reagent combination according to claim 1, wherein according to parts by weight,

Second reagent includes 0.1-4 parts of tetrahydro methylpyrimidine carboxylic acids and 0.1-30 parts of ceramides.

4. reagent combination according to claim 1, wherein according to parts by weight,

The third reagent includes 0.05-1 parts of macromolecular Sodium Hyaluronates, 0.05-1 parts of low-numerator sodium hyaluronates, 0.05-1 Part hydrolysis Sodium Hyaluronate, 0.1-15 parts of glycine betaines and 0.1-60 parts of ethoxy ureas.

5. reagent according to claim 1 combination, the reagent combination also includes grease, emulsifier, preservative and water, In,

The grease is selected from grape seed oil, corn oil, sesame oil, soybean oil, peanut oil, sunflower seed oil, saualane, poly- diformazan One of radical siloxane, myristic acid, palmitinic acid, stearic acid, beeswax, paraffin and vaseline are a variety of；

The emulsifier is selected from PPG-5- ceteth -20, stereth -2, stereth -21, cetearyl Portugal Glucosides, ceteth -20, ceteareth -25, polyglycereol-3-methyl distearate, Natrulon H-10 are stearic Acid esters, Natrulon H-10 myristinate, -6 lauric acid of Natrulon H-10 isostearate, Natrulon H-10 oleate and polyglycereol One of ester is a variety of；

The preservative be selected from methyl hydroxybenzoate, Nipasol, Phenoxyethanol, glycerol caprylate, Sensiva SC50, benzoic acid, One of sodium benzoate, sorbic acid and potassium sorbate are a variety of.

6. a kind of reagent for skin care combines, the reagent combination includes the first reagent, the second reagent and the

Three reagents, wherein according to parts by weight,

First reagent includes 0.1-4 parts of carnosines, 0.1-20 parts of animal umbilical cord extracts, 0.1-15 parts of golden button flower extracts Yeast tunning lysis object is split with 0.1-80 part two；

Second reagent includes 0.1-4 parts of tetrahydro methylpyrimidine carboxylic acids and 0.1-30 parts of ceramides；

The third reagent includes 0.05-1 parts of macromolecular Sodium Hyaluronates, 0.05-1 parts of low-numerator sodium hyaluronates, 0.05-1 Part hydrolysis Sodium Hyaluronate, 0.1-15 parts of glycine betaines and 0.1-60 parts of ethoxy ureas.

7. reagent according to claim 6 combination, the reagent combination also includes grease, emulsifier, preservative and water, In,

The grease is selected from grape seed oil, corn oil, sesame oil, soybean oil, peanut oil, sunflower seed oil, saualane, poly- diformazan One of radical siloxane, myristic acid, palmitinic acid, stearic acid, beeswax, paraffin and vaseline are a variety of；

The emulsifier is selected from PPG-5- ceteth -20, stereth -2, stereth -21, cetearyl Portugal Glucosides, ceteth -20, ceteareth -25, polyglycereol-3-methyl distearate, Natrulon H-10 are stearic Acid esters, Natrulon H-10 myristinate, -6 lauric acid of Natrulon H-10 isostearate, Natrulon H-10 oleate and polyglycereol One of ester is a variety of；

The preservative be selected from methyl hydroxybenzoate, Nipasol, Phenoxyethanol, glycerol caprylate, Sensiva SC50, benzoic acid, One of sodium benzoate, sorbic acid and potassium sorbate are a variety of.

8. a kind of preparation method that the reagent for skin care combines, wherein the method comprises the following steps:

S1. by macromolecular Sodium Hyaluronate, low-numerator sodium hyaluronate, hydrolysis Sodium Hyaluronate, glycine betaine, ethoxy urea and water It is added in water phase pot and is uniformly mixed, be heated to 75-85 DEG C of heat preservation 20-30min to being completely dissolved, obtain mixture A；

S2. emulsifier and grease are added to oily phase pot to be uniformly mixed, are heated to being completely melt, until 75-85 DEG C, keep the temperature 20- 30min obtains oily phase B；

S3. the oil phase B and mixture A is uniformly mixed in emulsion pot, at 70-80 DEG C of emulsifying 10-20min points Clock, and vacuum defoamation is carried out simultaneously, obtain mixture C；

S4. carnosine, animal umbilical cord extract, golden bachelor's button is added after the mixture C cold bath being cooled to 35-45 DEG C to extract Object, two split yeast tunning lysis object, tetrahydro methylpyrimidine carboxylic acid, ceramide and preservative, and 50-60r/min stirring is equal It is even, 33-38 DEG C of discharging.

9. a kind of skin-protection product, wherein the skin-protection product includes the described in any item reagent combinations of claim 1-7.

10. the described in any item reagent combinations of claim 1-7 are preparing the purposes in skin-protection product or pharmaceutical composition.