CN111110623A

CN111110623A - Self-repairing small needle nutrient solution formula

Info

Publication number: CN111110623A
Application number: CN201910577611.5A
Authority: CN
Inventors: 孙定邦
Original assignee: Qingdao Huaao Modern Medical Beauty Co ltd
Current assignee: Qingdao Huaao Modern Medical Beauty Co ltd
Priority date: 2019-06-28
Filing date: 2019-06-28
Publication date: 2020-05-08

Abstract

The invention belongs to the technical field of cosmetology, and particularly relates to a self-repairing small needle nutrient solution formula which comprises self-collagen, stem cell suspension, hyaluronic acid solution, epidermal growth factor solution and antioxidant molecules, wherein the self-repairing small needle nutrient solution comprises the following components in parts by weight: 35-45 parts of collagen, 35-45 parts of stem cell suspension, 3-8 parts of hyaluronic acid solution, 3-8 parts of epidermal growth factor solution, 3-8 parts of vitamin E solution and 3-8 parts of antioxidant molecules, and further comprises platelet-rich plasma, wherein the mass parts of the platelet-rich plasma are 20 parts, and the collagen comprises antelope apatite, polylactic acid, polynucleotide and fibroblast, and the mass parts of the components are as follows: 5 parts of antelope-base apatite, 5 parts of polylactic acid, 5 parts of polynucleotide and 10 parts of fibroblast. The invention has the beneficial effects that: the formula is reasonable in arrangement, the functions are various, the self-repairing capability of the skin can be effectively activated, the skin-care product is safe, effective and risk-free, applicable parts are from the face to the body, and the items are complete.

Description

Self-repairing small needle nutrient solution formula

Technical Field

The invention belongs to the technical field of beauty treatment, and particularly relates to a formula of an autologous-repair small-needle nutrient solution.

Background

Minimally Invasive Medicine (MIM) has become a trend in medical development, both in the dermatological and other medical fields, and broadly, it includes surgical treatment of all small incisions and minor wounds. The minimally invasive beauty treatment is a minimally invasive technology adopted for medical beauty treatment, such as chemical exfoliation, laser and radio frequency, injection filling technology, endoscopic treatment and minimally invasive beauty surgery, and is characterized by small wound, safety, rapidness, light pain and quick recovery. Therefore, minimally invasive beauty is favored by more and more people. Injection cosmetology is another important area of minimally invasive medical cosmetology emerging after laser cosmetology in China. It is a beautifying method for beautifying face contour and improving skin texture by means of injection to obtain youthful and beautified face. For patients, the injection cosmetic has little pain, safety, effectiveness, quick effect, short or no downtime. Therefore, injection beauty treatment is an emerging beauty treatment technology which is rising and developing rapidly in our country at present, and becomes a new field with great development potential in dermatology.

However, the existing repair nutrient solution for injection beauty has single function and cannot effectively activate the self-repair capability of skin.

Disclosure of Invention

In order to solve the problems, the invention provides a self-repairing small needle nutrient solution formula.

The technical scheme of the invention is as follows: the self-repairing small needle nutrient solution formula comprises self-collagen, and is characterized by also comprising a stem cell suspension, a hyaluronic acid solution, an epidermal growth factor solution and an antioxidant molecule, wherein the weight parts of the components are as follows: 35-45 parts of collagen, 35-45 parts of stem cell suspension, 3-8 parts of hyaluronic acid solution, 3-8 parts of epidermal growth factor solution, 3-8 parts of vitamin E solution and 3-8 parts of antioxidant molecules.

Preferably, the mass parts of each group are as follows: 40 parts of collagen, 40 parts of stem cell suspension, 5 parts of hyaluronic acid solution, 5 parts of epidermal growth factor solution, 5 parts of vitamin E solution and 5 parts of antioxidant molecules.

Preferably, the blood-platelet-rich plasma is also included, and the mass part is 20 parts.

Preferably, the feed also comprises antelope apatite, polylactic acid, polynucleotide and fibroblast, and the components in parts by weight are as follows: 5 parts of antelope-base apatite, 5 parts of polylactic acid, 5 parts of polynucleotide and 10 parts of fibroblast.

Preferably, the collagen is autologous collagen;

preferably, the antioxidant molecule is one or more of superoxide dismutase, protocatechuic acid, ascorbic acid and brown algae polysaccharide.

Preferably, the stem cell suspension adopts adipose-derived stem cell suspension, the adipose-derived stem cells comprise suspension adipose-derived stem cells and normal saline, the concentration of the adipose-derived stem cell suspension is 1 × 106-1 × 107/mL, and the adipose-derived stem cells are prepared by separating and culturing adipose tissues of a human body;

the concentration of the hyaluronic acid solution adopts 0.5 percent hyaluronic acid solution;

the epidermal growth factor solution concentration adopts 50-100 mug/ml epidermal growth factor solution;

the vitamin E solution has a concentration of 10mg/ml

Preferably, the preparation method of the autologous collagen comprises the steps of collecting blood by a blood collection method, allowing the blood to enter a whole blood separator after primary centrifugation, performing secondary centrifugation in the whole blood separator, dividing the whole blood into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, guiding the liquids of the plasma layer, the white membrane layer and the red blood cell layer into different storage units through catheters for separate storage, taking out a proper amount of the liquids of the plasma layer and the white membrane layer from the storage units, mixing the liquids in proportion, performing primary purification through a chromatography unit, further purifying through a diafiltration unit to obtain the required autologous collagen, and refrigerating the autologous collagen.

Preferably, the platelet rich plasma is prepared by centrifuging autologous peripheral blood to obtain autologous plasma layer and cell layer; and centrifuging and concentrating the autologous plasma layer to obtain platelet-rich plasma, keeping the platelet-rich plasma in an inactivated state, and storing the platelet-rich plasma at a low temperature.

Preferably, the polynucleotide is extracted from fresh fish germ cells;

the fibroblast is fibroblast of human skin.

Preferably, the polylactic acid is levorotatory polylactic acid;

the antelope base apatite is antelope base apatite calcium.

The invention has the beneficial effects that: the formula is reasonable in arrangement, the self-repairing capability of the skin can be effectively activated, the skin is safe, effective and risk-free, applicable parts are from the face to the body, and the project is complete.

Detailed Description

An embodiment of the present invention is explained below.

The self-repairing small needle nutrient solution formula comprises self-collagen, stem cell suspension, hyaluronic acid solution, epidermal growth factor solution and antioxidant molecules, and the self-repairing small needle nutrient solution formula comprises the following components in parts by weight: 35-45 parts of collagen, 35-45 parts of stem cell suspension, 3-8 parts of hyaluronic acid solution, 3-8 parts of epidermal growth factor solution, 3-8 parts of vitamin E solution and 3-8 parts of antioxidant molecules. The formula is reasonable in arrangement, the self-repairing capability of the skin can be effectively activated, the skin is safe, effective and risk-free, applicable parts are from the face to the body, and the project is complete.

The weight parts of each group are as follows: 40 parts of collagen, 40 parts of stem cell suspension, 5 parts of hyaluronic acid solution, 5 parts of epidermal growth factor solution, 5 parts of vitamin E solution and 5 parts of antioxidant molecules.

Also comprises platelet-rich plasma, and the mass portion is 20 portions.

The composition also comprises antelope-base apatite, polylactic acid, polynucleotide and fibroblast, and the components are as follows by weight: 5 parts of antelope-base apatite, 5 parts of polylactic acid, 5 parts of polynucleotide and 10 parts of fibroblast.

The collagen adopts autologous collagen; the autologous collagen can be used for replacing the original high-price filler. Meanwhile, the medicament also has various advantages of no toxicity, no side effect and the like. At the same time, PRP (also called serum active growth factor) can be extracted and injected into the dermal layer of the skin. Promoting the aged skin to quickly recover the elasticity, and helping people dispel wrinkles, improve the face shape, shrink pores and tenderize and smooth the skin.

The antioxidant molecule is one or more of superoxide dismutase, protocatechuic acid, ascorbic acid and brown algae polysaccharide. Substances that prevent the adverse effects of oxygen. It is a class of substances that helps to trap and neutralize free radicals, thereby removing the damage that free radicals cause to the human body.

Superoxide dismutase (SOD) is an important component of an antioxidant enzyme system in a biological system and is widely distributed in microorganisms, plants and animals. Skin aging and damage are important features of human aging, which is a consequence of obstacles to the accumulation or removal of reactive oxygen species free radicals, which cause cellular damage and pigmentation. Since human skin is directly exposed to oxygen, aging and damage of the skin may be caused. The supplement of the exogenous SOD is beneficial to delaying skin aging, resisting oxidation and removing color spots.

Protocatechuic acid (PCA), 3, 4-dihydroxybenzoic acid, is a phenolic acid substance naturally present in many vegetables and fruits, and is an effective active ingredient in many traditional Chinese medicines (such as salvia miltiorrhiza, hibiscus, etc.). Protocatechuic acid is a water-soluble phenolic acid component, is an active substance of a plurality of traditional Chinese medicines, has pharmacological activities of resisting platelet aggregation, reducing myocardial oxygen consumption, improving myocardial oxygen resistance, slowing heart rate, inhibiting bacteria, relieving pain and the like, and also has antioxidant, anti-tumor and neuroprotective effects.

Ascorbic acid is generally vitamin C, which is structurally similar to glucose and is a polyhydroxy compound whose two adjacent enol-type hydroxyl groups at the 2 nd and 3 rd positions in the molecule are easily dissociated to release H⁺Therefore, it has the property of acid, also called ascorbic acid. Vitamin C has strong reducibility and is easily oxidized into dehydrovitamin C, but the reaction is reversible, and ascorbic acid and dehydroascorbic acid have the same physiological function, but if the dehydroascorbic acid is continuously oxidized to generate diketogulonic acid, the reaction is irreversible and the physiological effect is completely lost.

The fucoidan is mainly derived from sea algae such as herba Zosterae Marinae, Macrocystis, Ascophyllum nodosum, and Fucus vesiculosus, and comprises algin, fucoidan, and alginate starch. Excessive reactive oxygen radicals destroy phagocytes themselves and other cells, tissues and biological macromolecules, and accelerated lipid peroxidation can cause normal cell destruction and death. Algal polysaccharide not only has the function of eliminating active oxygen, but also can obviously reduce the content of Lipid Peroxide (LPO), improve the activity of peroxidase (CAT) and superoxide dismutase (SOD), has the functions of eliminating excessive free radicals and resisting lipid peroxidation, and can obviously enhance the capability of resisting oxidation and free radical damage of organisms, and the mechanism of the algal polysaccharide can enhance the capability of the organisms to eliminate free radicals by promoting the biosynthesis of the organisms to SOD, GSH-Px (glutathione peroxidase), GSH and the like.

The stem cell suspension adopts adipose-derived stem cell suspension, the adipose-derived stem cells comprise suspension adipose-derived stem cells and normal saline, the concentration of the adipose-derived stem cell suspension is 1 × 106-1 × 107/mL, and the adipose-derived stem cells are prepared by separating and culturing adipose tissues of a human body; adipose-derived stem cells (ADSCs) and ADSC pluripotent cells are stem cells with a multipotential differentiation potential which have been separated from adipose tissues in recent years. Mainly recovers the repairing function of tissue cells, promotes the regeneration of cells, recovers the young face, simultaneously fully improves the body function, effectively improves diseases such as sub-health, premature senility and the like, and really and effectively resists aging from inside to outside.

The hyaluronic acid solution is 0.5 percent hyaluronic acid solution in concentration, the hyaluronic acid belongs to natural components of a human body, is essentially acid salary polysaccharide formed by disaccharide monomers (glucosamine and glucuronic acid) through aggregation, has the function of stabilizing extracellular matrix, has strong water absorption capacity, can lock water and keep skin elasticity, has stable structural rigidity, proper biocompatibility and degradability, and basically has no antigen specificity, stimulates fibroblasts to generate collagen, ② binds water and stimulates a fat tissue reaction, ③ supports and activates fatty ten cells.

Hyaluronic acid is commonly used for volume filling in the face and the back, filling glume, nose augmentation, lip enlargement and the like, filling depressions or fatigue marks, correcting small symmetry, improving soft tissue atrophy and the like, has good effects, improves the proportion of five sense organs to a certain extent, and reduces the risks of open surgery and prosthesis installation. Recently HA HAs also been used to enhance the effect of total and multiple channel intradermal microinjection, modify fine wrinkles and depressions, improve skin quality and restore skin thickness, stimulate collagen proliferation, and achieve rejuvenation.

epidermal Growth Factor (EGF) is a heat-resistant single-chain low-molecular polypeptide consisting of 53 amino acid residues. After EGF is specifically identified and combined with EGF receptor on target cells, a series of biochemical reactions occur, and finally, the DNA synthesis and mitosis of the target cells can be promoted. EGF is free of sugar, is very stable, heat-resistant and acid-resistant, is widely present in body fluids and various glands, is mainly synthesized by submandibular glands and duodenum, and is found in most body fluids of human bodies in a content specifically increased in milk, urine and semen, but in a lower concentration in serum. EGF has wide effect and is of great significance in estimating tumor prognosis, selecting treatment schemes, treating gastric ulcer, liver failure and the like. The EGF content in serum or body fluid can be clinically measured by a Radioimmunoassay (RIA) or a double-antibody enzyme-linked immunosorbent assay (ELISA).

The vitamin E solution has a concentration of 10mg/ml

The preparation method of the autologous collagen comprises the steps of collecting blood by a blood collection device, allowing the blood to enter a whole blood separator after primary centrifugation, performing secondary centrifugation in the whole blood separator, dividing the whole blood centrifugation into three layers, namely a plasma layer, a white membrane layer and a red blood cell layer from top to bottom, guiding the liquids of the plasma layer, the white membrane layer and the red blood cell layer into different storage units through catheters for separate storage, taking out a proper amount of the liquids of the plasma layer and the white membrane layer from the storage units, mixing the liquids in proportion, performing primary purification through a chromatography unit, further purifying through a diafiltration unit to obtain the required autologous collagen, and refrigerating the autologous collagen.

The preparation method of platelet rich plasma comprises centrifuging autologous peripheral blood to obtain autologous plasma layer and cell layer; and centrifuging and concentrating the autologous plasma layer to obtain platelet-rich plasma, keeping the platelet-rich plasma in an inactivated state, and storing the platelet-rich plasma at a low temperature.

Platelet-rich plasma is highly concentrated autologous plasma obtained by in vitro centrifugation of whole blood [ fso7, which contains a large amount of platelets, and activated platelets can release a large amount of growth factors including platelet-derived growth factors, vascular endothelial growth factors, fibroblast growth factors and the like, and has the effects of regulating cell proliferation and differentiation, promoting vascular proliferation and inflammatory chemotaxis and the like. At present, other methods such as AFT are often combined to promote vascularization of transplanted fat and improve survival rate; the laser can be applied to skin rejuvenation in a combined manner, so that a better curative effect circle can be obtained; PRP alone can improve the wrinkles, depressions and dark circles around the eyes and increase the elasticity of local tissues. The minimum requirement for PRP is quantified in order to obtain better comfort during treatment.

Extracting polynucleotide from germ cell of fresh fish; purified Polynucleotide (PN) extracted from salmon embryo cells is a novel bulking agent ingredient in the Central European region and in Korea. The novel product containing the polynucleotide component can not only realize the filling of the loss component, but also can promote the activation of the self-regeneration capability of damaged tissues and cells and promote the natural regeneration of the tissues, so the novel product is called as a new concept skin regeneration therapeutic agent, namely a retrogradation needle. PN molecules are polymer chains formed by covalently bonding 13 or more nucleotide monomers. The use of long chain PN molecules in the treatment of skin rejuvenation is based on a certain research basis. In recent years, research shows that PN can promote the repair and healing of bone tissues, and in the case of low perfusion of tissues after diabetes and burns, PN can promote the synthesis of vascular endothelial cell growth factors so as to be beneficial to the formation of new blood vessels and the repair of wounds. In the skin field, PN can promote the growth of human corneal fibroblasts and the repair of dermal fibroblasts after UVB injury, and can stimulate primary cultured fibroblasts to secrete collagen and other extracellular matrix proteins, accelerate the metabolism of the fibroblasts and promote the regeneration of dermal components.

The fibroblast is fibroblast of human skin. The autologous fibroblast is a method for preparing autologous fibroblast by using skin fibroblast precursor cells, relates to the application of a cell engineering technology, and the generated fibroblast can be used for cell transplantation. Belongs to the technical field of biological engineering.

Fibroblasts are the main cellular component of skin. Fibroblasts have the function of synthesizing and secreting three kinds of fiber APSC multipotential cells and stroma. Has very important function in the process of updating the interstitium and repairing the wound.

The polylactic acid is L-polylactic acid; the levorotatory polylactic acid is a degradable artificial mediating biomaterial which is approved by FDA and has safety and excellent filling effect. Has the advantages of good biocompatibility, low toxicity, easy modification and the like, achieves the purpose of beautifying and filling by stimulating fibroblasts to secrete collagen, and needs regular massage after injection to prevent the formation of nodules.

The antelope base apatite class adopts antelope base apatite calcium which is a high polymer calcium phosphate medium polymer with high cross medium concentration, is widely existed in human bone tissues, has the advantages of excellent biocompatibility shared by polymer fillers and manual shaping in a short time after injection, is used for filling deeper tissues such as nasolabial sulcus, hump nose and the like for a second time in the United states for a long time, obtains similar effect and satisfaction degree in contrast research with hyaluronic acid, and has good application prospect.

Example 1

The preparation method of the self-repairing small needle nutrient solution comprises the steps of culturing and obtaining autologous adipose-derived stem cells from autologous fat, adding a hyaluronic acid solution, an epidermal growth factor solution and an antioxidant molecule, uniformly mixing, and adding the autologous adipose-derived stem cells to form the self-repairing small needle nutrient solution.

Example 2

The self-repairing small needle nutrient solution formula comprises self-collagen, stem cell suspension, hyaluronic acid solution, epidermal growth factor solution, antioxidant molecules and platelet-rich plasma, and the self-repairing small needle nutrient solution formula comprises the following components in parts by weight: 35-45 parts of collagen, 35-45 parts of stem cell suspension, 3-8 parts of hyaluronic acid solution, 3-8 parts of epidermal growth factor solution, 3-8 parts of vitamin E solution and 3-8 parts of antioxidant molecules. The formula is reasonable in arrangement, the self-repairing capability of the skin can be effectively activated, the skin is safe, effective and risk-free, applicable parts are from the face to the body, and the project is complete.

The preparation method of the self-repairing small needle nutrient solution comprises the steps of obtaining self-adipose stem cells by culturing from self-adipose, obtaining blood plasma rich in platelets from peripheral blood of a body, adding a hyaluronic acid solution, an epidermal growth factor solution and antioxidant molecules, mixing uniformly, and adding the self-adipose stem cells to form the self-repairing small needle nutrient solution.

Example 3

The self-repairing small needle nutrient solution formula comprises self-collagen, stem cell suspension, hyaluronic acid solution, epidermal growth factor solution, antioxidant molecules and platelet-rich plasma, and the self-repairing small needle nutrient solution formula comprises the following components in parts by weight: 40 parts of collagen, 40 parts of stem cell suspension, 5 parts of hyaluronic acid solution, 5 parts of epidermal growth factor solution, 5 parts of vitamin E solution, 5 parts of antioxidant molecules and 20 parts of platelet-rich plasma.

Example 4

The vitamin E solution has a concentration of 10mg/ml

The small needle action principle: the micro-needle is a hollow needle with the diameter of 40-80 μm, and the micro-needle with the diameter of less than 40 μm adopted by the small micro-needle has smaller diameter compared with the conventional micro-needle,

the skin nutrient solution is directly conveyed to the subcutaneous part by using the micro-needle with the same thin hair (the diameter is less than 40 mu m), a wound surface is formed under the subcutaneous part by soft stripping of water solution, the nutrient solution can automatically avoid capillary vessels and nerves, new collagen fibers and viscous chain protein, also called as adhesive between cells, can be continuously generated by the skin in the process of repairing, and the subcutaneous thickness is continuously increased by 3-6 times of treatment course, so that the effects of plumping, removing wrinkles, compacting and improving are achieved.

The small needle is thin and sharp, the puncture depth is only in the horny layer and the upper layer of the dermis, the needle does not contact nerve endings, the damage degree to organism tissues is reduced, the dermis layer is stimulated, and the collagen hyperplasia is promoted through the self-healing capacity of the skin, so that the self-repairing capacity of the skin can be effectively activated. The small needle is safe and efficient in the mode of introducing medicines through the small needle without irritation, and is the best mode for painless medicine administration.

The use method of the self-repairing small needle nutrient solution comprises the following steps:

injecting a compound nutrient solution rich in high-concentration collagen and containing a large amount of physiological saline into the subcutaneous part to supplement nutrition lost by the skin, so that the skin cells which are originally shriveled and atrophied become full, the skin environment is improved, and the skin is enabled to be vigorous;

and step two, the self-repair of the small needle by utilizing the anti-aging new technology is performed, the needle head and the needle handle are integrated into a syringe, the nutrient solution is injected to the fascia layer by adhering to the periosteum at the key point of facial tightening and lifting, and the fascia layer is separated by means of the hydrodynamic soft stripping effect of the liquid. The skin tissue will heal after it has suffered a wound, and scars will be formed during the healing process. The hyperplasia ability of the scar can plump the collapse parts of the face with loose, concave and incomplete congenital development; the contracture force of the scar can lead the face to form a contracture net of the scar under the skin, and the contracture net can be used for resisting the gravity and tightening the skin without looseness and droop;

thirdly, the hyperplasia ability of scars can generate a large amount of scar fibers, and the contracture ability can tighten the skin from all directions. The face then forms a collagen fiber mesh with a surface formed by dots. Normal skin generally ages one year and more rapidly in the middle. After the self-repairing small needle nutrient solution is injected, a contracture net with a layer of scars is formed under the skin;

and fourthly, delaying the aging, and enabling the skin to be in a state of one year without being injected with the self-repairing small needle nutrient solution for 2 to 3 years or even longer.

The operation time of injecting the self-repairing small needle nutrient solution is very fast, and only 10-20 minutes is needed for operating one part; according to the activity of cells and the aging speed, the sinking effect of the small needle can be maintained for at least 3-5 years, wrinkles can be maintained for 1-2 years, and a treatment course is improved for 2-3 years.

The self-repairing small needle nutrient solution can realize three anti-aging steps, and specifically comprises the following steps:

the first step, realizing youth freeze-frame, such as 35 years old of the customer, and if she wants to maintain such a state, the customer can freeze-frame after one treatment course, and freeze-frame is carried out once a year;

second, adolescent reversal, such as a customer being 40 years old, wanting a young 5 years old, returning to a 35 or even 30 year old state, would be done for one to two sessions, with a package designed to best suit her in light of the customer's questions and requirements;

and thirdly, local finishing carving is carried out, wherein the later maintenance and maintenance are carried out on younger customers or customers suitable for reversion.

The operation of the small needle does not influence normal work and rest; after operation, water is prevented from being stained for 24-48 hours, the operated part is kept free from being squeezed within 3 days, pungent and seafood and other hair substances are avoided for one week, and cold compress is preferably matched for the first 12 hours.

Compared with the prior art, the self-repairing small needle nutrient solution has various formula functions, can effectively activate the self-repairing capability of skin, is safe, effective and risk-free, has complete projects from the surface to the body of an applicable part, can be maintained by using the self-repairing small needle nutrient solution for a long time, can be always aged, and can prevent the aging problems of wrinkles, depressions, soft subcutaneous tissues and the like, and is concretely as follows;

(1) applied to the face, remold the young outline, make exquisite full little V face, the whole face is anti-ageing, and the concrete expression is:

improving true wrinkles, prolapse, collapse, relaxation, and deformation of contour;

improving four large lines, namely forehead lines, eye horn lines, statute lines and neck lines;

improving four small lines, namely, Sichuan line, nose back line, sawtooth line and ear front and ear back line;

improving four kinds of collapse, namely forehead, temples, eye pouch ditches and cheeks;

the upper eyelid, apple muscle, double chin and infant fat which are the four prolapses are improved;

the technical problems recognized by the international medical and American communities of mouth angle drop, mouth angle bag, nasal wing invagination, eye external angle sloughing, retraction and the like are solved;

improving cheek recession, performing cheek fat removal and lifting, improving puppet line recession, performing apple muscle lifting, improving statute line recession, improving plump apple muscle, improving puppet line, and lifting mouth corners;

the forehead can be used for enlarging and beautifying the human tip, and improving the Chinese character 'chuan' lines, the Yintang pits, the Taiyang pits and the forehead pits, so as to lift the canthus and repair the lines.

(2) The eye wrinkle removing liquid is applied to the eyes, wrinkles on the upper eyelids, inner canthus and eyelash roots can be removed in a 360-degree all-around mode, and gemmy bright-eye and large-eye are restored;

improving upper eyelid wrinkle, upper eyelid depression, and canthus lines, and performing upper eyelid lifting, eye lifting, eyebrow thickening, lacrimal groove leveling, eye fundus leveling, and pouch removing.

(3) The neck beautifying powder is applied to the neck, effectively removes wrinkles aiming at wrinkles, proud flesh and turkey necks of the neck, and reforms a rounded and smooth jade neck.

(4) The hand cream is applied to hands, the hand is repaired to be dry, the elastic force is restored, and the round and smooth jade fingers like the pink carving jade carving are reproduced;

(5) applied to shoulder and back to beautify the back, so that the shoulder and the back of a cow recover the back of a maiden, and a angel wing is made;

(6) the preparation method is applied to the private part, and the private part is recovered to a compact, elastic and moist state aged eighteen years;

(7) applied to others, such as earlobe enlargement, full face whitening dialysis (7 day whitening).

The self-repairing small needle nutrient solution has a safe formula, repairs and regenerates own fibrous tissues, does not need operation, enables the natural and natural beauty of the beauty to be invisible, and enables the young to become a life style.

The application relates to a difference that autologous collagen that self-repairing small needle nutrient solution produced has apparent difference with hyaluronic acid, hyaluronic acid is also called uronic acid, hyaluronic acid, divide into the micromolecule, the mesomolecule, the macromolecule, the micromolecule is often used for making the water light, the mesomolecule macromolecule is often used for filling facial depression, hyaluronic acid is human inseparable partly, can be absorbed by autodegradation, macromolecule holding time is 8 months to a year, need inject repeatedly, the injection position can appear different facial changes along with hyaluronic acid's absorption, and the self-repairing small needle nutrient solution formula that this application relates to can impel autologous cell growth, it is the tissue of oneself to grow out, the holding time is relatively long, it is natural.

The application relates to the difference between autologous collagen generated by the self-repairing small needle nutrient solution and the wrinkle removal with botulinum toxin: botulinum toxin, also known as botulinum toxin, has the effect of removing dynamic wrinkles, and can not be resolved by botulinum toxin, which forms true wrinkles in the form of ravines. The short duration of the botulinum toxin requires repeated injections; the small needle stimulates the growth of collagen cells of the small needle, directly smoothes wrinkles, lasts for a long time, and enables a person to return to ten years ago.

The application relates to a difference of self-repairing small needle and ultrasonic knife: the embodiment of aging: the whole muscle tissue relaxes and sags, collagen runs off, subcutaneous fat atrophy and collapse, wrinkles and cell water locking function is reduced, aiming at the symptoms, the ultrasonic knife solves the problems of whole muscle relaxation and sag to achieve the efficacy of fascia anti-aging, and we can find that the ultrasonic knife has obvious effect on the relaxation of the thickness of subcutaneous fat layer but has general effect on the state of thin subcutaneous fat, therefore, the comprehensive young state is really needed to be achieved, the strong supporting force of the fascia layer is not needed, the skin is tight, meanwhile, the skin tissue structure is also needed to be full, the size is simple, the thin shape does not represent tight and does not represent good looking, the lines are good looking, therefore, the full face can increase the three-dimensional contour sense of the whole face, the small needles solve the problem of full subcutaneous tissue, the fascia stop of the ultrasonic knife is a double-layer means for really restoring the skin to be young, and the skin is really young only if the skin is full enough.

The self-repairing small needle related to the application adopts a skin nutrition awakening technology to improve the nutrition environment of skin, then carries out point-to-point deep supplement, generates collagen necessary for a human body, accelerates cell propagation and self-growth, activates the self-repairing regeneration capacity of the skin, generates self-repairing growth factors and collagen, and has no side effect.

Compared with the prior art, the formula is reasonable in arrangement, multiple in functions, safe, effective and risk-free, can effectively activate the self-repairing capability of skin, and is suitable for the skin from the face to the body, and the items are complete.

The present invention has been described in detail with reference to the examples, but the present invention is only preferred examples of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.

Claims

1. The self-repairing small needle nutrient solution formula comprises self-collagen, and is characterized by also comprising a stem cell suspension, a hyaluronic acid solution, an epidermal growth factor solution and an antioxidant molecule, wherein the weight parts of the components are as follows: 35-45 parts of collagen, 35-45 parts of stem cell suspension, 3-8 parts of hyaluronic acid solution, 3-8 parts of epidermal growth factor solution, 3-8 parts of vitamin E solution and 3-8 parts of antioxidant molecules.

2. The self-repairing small needle nutrient solution formula according to claim 1, which is characterized in that the self-repairing small needle nutrient solution formula comprises the following components in parts by weight: 40 parts of collagen, 40 parts of stem cell suspension, 5 parts of hyaluronic acid solution, 5 parts of epidermal growth factor solution, 5 parts of vitamin E solution and 5 parts of antioxidant molecules.

3. The self-repairing small needle nutrient solution formula as claimed in claim 1 or 2, characterized by further comprising platelet-rich plasma in a mass portion of 20 parts.

4. The self-repairing small needle nutrient solution formula as claimed in claim 3, further comprising antelope-apatite, polylactic acid, polynucleotide, and fibroblast, wherein the weight parts of the components are as follows: 5 parts of antelope-base apatite, 5 parts of polylactic acid, 5 parts of polynucleotide and 10 parts of fibroblast.

5. The self-repairing small needle nutrient solution formula as claimed in claim 1 or 2, wherein the collagen is self-collagen;

the antioxidant molecule is one or more of superoxide dismutase, protocatechuic acid, ascorbic acid and brown algae polysaccharide.

6. The self-repairing small needle nutrient solution formula as claimed in claim 1 or 2, wherein the stem cell suspension is adipose stem cell suspension, the adipose stem cells comprise suspension adipose stem cells and normal saline, the concentration of the adipose stem cell suspension is 1 x 106-1 x 107/mL, and the adipose stem cells are prepared by isolated culture in human adipose tissues;

the concentration of the vitamin E solution is 10mg/ml of the vitamin E solution.

7. The self-repairing small needle nutrient solution formula as claimed in claim 5, wherein the preparation method of the autologous collagen comprises collecting blood from a human body by blood collection, subjecting the blood to a first centrifugation, then feeding the blood into a whole blood separator, subjecting the blood to a second centrifugation in the whole blood separator, wherein the whole blood centrifugation is divided into three layers, namely a plasma layer, a leukocyte layer and a erythrocyte layer from top to bottom, introducing the liquids of the plasma layer, the leukocyte layer and the erythrocyte layer into different storage units through catheters for separate storage, taking out a proper amount of the liquids of the plasma layer and the leukocyte layer from the storage units, mixing the liquids in a certain proportion, subjecting the liquids to a primary purification by a chromatography unit, further purifying by a diafiltration unit to obtain the required autologous collagen, and refrigerating the autologous collagen.

8. The self-repairing small needle nutrient solution formula as claimed in claim 2 or 3, wherein the platelet-rich plasma is prepared by centrifuging autologous peripheral blood to obtain autologous plasma layer and cell layer; and centrifuging and concentrating the autologous plasma layer to obtain platelet-rich plasma, keeping the platelet-rich plasma in an inactivated state, and storing the platelet-rich plasma at a low temperature.

9. The self-repairing small needle nutrient solution formula as claimed in claim 4, wherein the polynucleotide is extracted from germ cells of fresh fish;

the fibroblast is fibroblast of human skin.

10. The self-repairing small needle nutrient solution formula as claimed in claim 5, wherein the polylactic acid is L-polylactic acid;

the antelope base apatite is antelope base apatite calcium.