KR101678621B1 - Composition comprising spicules for skin care - Google Patents

Abstract

The present invention provides a method of preparing a speckle comprising: preparing a speckle; (EGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), acetylhexapeptide-8, copper tripeptide-1, Preparing a composition by mixing a plant extract fermentation solution and the like; And an additive mixture to prepare a cosmetic composition. The present invention also relates to a cosmetic composition for skin care.

Description

[0001] Cosmetic compositions for skin care comprising spicules and methods for producing the same [0002]

The present invention relates to a cosmetic composition using a speckle and a method for producing the same, and more particularly, to a cosmetic composition using a speckle and a method for producing the same, which comprises an epithelial cell growth factor (EGF), a fibroblast growth factor (FGF), an insulin- (AAPE), a plant stem cell extract, and the like, and a method for producing cosmetics for skin care or skin care.

Skin has been known to cause senescence, dullness and wrinkles due to external stimuli such as cell deformation and active oxygen, but the exact cause has not been revealed. However, efforts are being made to identify mechanisms for various major causes. Aging is caused by the formation of wrinkles in the skin, loss of elasticity and formation of spots. Usually, when the skin is damaged in childhood, no scar is left, but when the skin is damaged due to age, the scar is easily left. And that the proliferation rate of metastasis-amplified cells decreases significantly with aging. That is, as the aging progresses, the proliferation rate of various cells decreases, resulting in skin regeneration ability, wrinkle formation, delayed wound healing rate, and reduced skin elasticity.

Accordingly, various skin care or massage cosmetic compositions have been proposed in order to prevent skin aging or to maintain a current level of skin condition. For example, Korean Patent Laid-Open Publication No. 2010-0104703 relates to a functional cosmetic composition for skin regeneration and a method for using the functional cosmetic composition for skin regeneration, which comprises a 5-aminolevulinic acid ester or a salt thereof as an active ingredient, The above-mentioned functional cosmetic composition for skin regeneration has been disclosed to be easy to percutaneously absorb when applied to skin, has a low cytotoxicity, and is excellent in skin regeneration effect, so that it is useful for prevention of aging and skin care and has excellent effects. No. 2011-0119062 discloses a composition for improving skin regeneration or wrinkles containing stem cell culture concentrate obtained by culturing human adipose-derived stem cells in a serum medium, subculturing in a serum-free medium, and then filtering the diluted stem cell culture concentrate as an active ingredient . WO 2005/034839 discloses a therapeutic composition derived from a sponge animal for treating and preventing skin diseases, which is a medical application of a spongy animal. It is used for treating skin diseases and diseases, ≪ / RTI > or a pharmaceutically acceptable salt thereof. Korean Patent No. 0937389 discloses a method for manufacturing a skin care cosmetic composition using a sea surface bone fragment, and a local skin care cosmetic comprising a coating component such as a stem cell-derived material of a food of Dong-A Food, a growth factor of an epithelial cell, have. However, the above-mentioned prior arts are concerned with a composition for skin management of local area by using spicule (sea surface skeleton) and stem cell-derived material as a therapeutic agent for skin regeneration by mixing or using them respectively, Thereby effectively inducing collagen production of the dermal layer and improving skin elasticity and improving skin tone.

The present invention relates to a cosmetic composition which uses a cancellous bone piece for delivering an effective ingredient to the skin and effectively manages the skin by mixing various ingredients capable of reducing skin harmful factors such as skin elasticity, skin tone, wrinkles, It is an object to provide a composition.

The present invention relates to a pharmaceutical composition for the treatment and / or prophylaxis of inflammatory diseases such as epithelial cell growth factor (EGF), fibroblast growth factor (FGF), insulin-like growth factor (IGF-1) and human adipose stem cell culture medium (AAPE) It is a system that directly transports the active ingredient by combining peptide-8, capper tripeptide-1, fermentation water (Streptococcus thermophilus / Angelica / Horse / Crude protein / Pomegranate / Omija extract fermentation filtrate) It is an object of the present invention to provide a cosmetic which can improve the regeneration ability of skin cells and solve various problems appearing on the skin.

The present invention relates to a method for preparing a cosmetic composition for skin care, comprising the steps of: preparing a speckle; (EGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), insulin-like growth factor (IGF-1) and human growth hormone (stem cell) And preparing a composition by mixing acetyl hexapeptide-8, Copper tripeptide-1, ginseng callus culture solution, and plant extract fermentation solution; Immersing the spicule in the mixed composition for 24 to 48 hours; Preparing a cosmetic composition by mixing the immersed spicule with an additive mixture in which a humectant, a skin protecting agent, a viscosity modifier, and an emulsifier are mixed; and a method of manufacturing a cosmetic composition for skin care.

In another aspect, the present invention relates to a method for the treatment and prophylactic treatment of speckle, a human adipose stem cell culture medium, a cell growth factor (CGF) epithelial cell growth factor (EGF), a vascular endothelial growth factor (VEGF) , Insulin-like growth factor (IGF-1), acetylhexapeptide-8, copper tripeptide-1, ginseng callus culture solution and plant extract fermentation liquid. More specifically, the plant extract fermentation broth is characterized by fermenting Puerariae radix, horse riding, pomegranate, Omija, and sugar beet with Streptococcus thermophillus, more preferably 10-20 parts by weight, It is appropriate that the human adipose tissue stem cell culture solution contains 20 to 30 parts by weight.

The present invention also relates to a method for the treatment and / or prophylaxis of a cell growth factor (EGF), a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGF) and an insulin-like growth factor (IGF- , Acetyl hexapeptide-8, copper tripeptide-1, ginseng callus culture solution, and plant extract fermentation solution are preferably mixed in the same parts by weight as 10 to 30 parts by weight of the composition, The composition may be further provided with a cosmetic composition by further adding an acidity controlling agent, a moisturizing agent, a skin protecting agent, an emulsifying agent and a viscosity controlling agent. In addition, the composition may further include lavender oil to improve the fragrance of cosmetics. Such a composition can be used as a composition which induces collagen production and has an effect of increasing skin elasticity, improving wrinkles and exfoliating effect.

The cosmetic composition of the present invention allows penetration into the dermis epithelium layer by using needle-shaped spicule extracted from the sponge body. At the same time, the human body fat stem cell culture fluid and various cell growth factors are mixed to form skin collagen , It can be effectively used as a cosmetic composition for skin care and / or massage, because it has a wrinkle improving effect, a pigmentation alleviation prevention and prevention, an acne improvement, and a fresh seaweed effect.

FIG. 1 is a view comparing the removal effect of the stratum corneum (A) and the post-use (B) before use.
Fig. 2 shows that the skin tone is brightened, the fine wrinkles are improved, and the deep wrinkles are thinned after (A) before use of the composition.

Hereinafter, preferred embodiments of the present invention will be described in detail. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. It will be obvious to those who have knowledge of. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

The sponge (sponge fragment), which is a major constituent of the present invention, is also called sponge, and sponge animal is a primitive marine creature having no muscles, nerves or organs, and has more than 5,000 species. It is a creature that is found. The skeleton is a tissue that functions as a skeleton that has a needle shape present in the body of invertebrate animals, and is mainly composed of silicic acid and calcium carbonate. The fibrous skeleton of sponges has absorption ability according to the capillary phenomenon and is applied to various fields such as medical use. The fragments of sponges are divided into sponge fragments containing antibiotics for the treatment of osteomyelitis, treatment of spongiform encephalopathies for the treatment and prevention of skin diseases, use of sponge fragments as a skin grinding device or skin regeneration means.

In the present invention, a stem cell is a primordial cell, which is an allogeneic cell capable of being converted into an organ, and is a cell having the ability to differentiate into various types of body tissues, also called "stem cells" Cells. It is an undifferentiated cell of the embryonic stage that has not yet differentiated and has stopped its differentiation before the formation of a specific organ, and has the ability to differentiate into different organ systems such as muscles, bones, and skin. Various studies have been carried out to apply the present invention to various treatments such as regeneration of injured tissues by appropriately adjusting the necessary and sufficient conditions in such undifferentiated state. In other words, stem cells are undifferentiated cells, and based on their self-renewal ability and strong differentiation ability, they offer great possibilities to regenerative medicine, and by using them, development of highly functional cosmetics with enhanced skin safety .

(AAPE), an active ingredient of the present invention, has been used in stem cell research called as a flower of bio-biotechnology in order to grow and differentiate stem cells in vitro. It plays a role in activating the dominant cells by creating a similar environment. In the process of culturing stem cells using these cultures, many nutrients are needed to grow the cells, and the cells that have absorbed the nutrients will release a large amount of growth factors, cytokines, and extracellular matrix proteins. The secreted culture solution is separated from the cells to use an effective ingredient in the culture solution.

In addition, the present invention includes a cell growth factor (CGF). The cell growth factor is a proteinaceous physiologically active substance, and various kinds are found. The variety is donated. It participates in cell signaling system, To participate. These growth factors mediate specific target receptors and act on their own target cells. Active ingredients such as various growth factors such as EGF, bFGF, and KGF IGF KGF PDGF, which are very important for skin health, help the overall skin troubles such as decrease in elasticity, dryness and roughness of skin, and make skin healthy and elastic. These cell growth factors are made nano-sized to act deeper into the skin, penetrating the skin layer and delivering it deep into the dermis.

Epithelial growth factor (EGF), which is another active ingredient in the present invention, is a polypeptide consisting of 58 amino acids. It is a component for improving endothelial cells and synthesizing collagen, which is an aqueous component of dermis. It is a natural skin improving substance in the human body. It is a protein with powerful regenerating function that heals skin wounds. It is also used as a therapeutic ingredient for normal skin regeneration after skin exfoliation. This ingredient gives vitality to the skin, brightens the skin tone, and smoothes and brightens the surface of the skin.

Vascular endothelial growth factor (VEGF), another active ingredient, induces cell growth and division, promotes vascular cell migration, and increases skin texture.

It has been found that fibroblast growth factor (FGF) contained in the present invention is one of the growth factor classes and forms a family of 23 kinds from the structural similarity. Of these, FGF-1 and FGF-2 promote strong cell division It is known to increase the skin density of the dermal layer of the skin. As the aging progresses, the recovery of the skin surface is delayed, but the thickness of the dermis, which is the lower layer of the epidermis, decreases as well, and the number of the dermal blood vessels decreases overall. At this time, it participates in the dermal layer to grow cells, and participates in the synthesis of a structural material such as collagen, elastin, and glycosaminoglycan, thereby making the skin structure loosened by the aged cells.

In addition, insulin-like growth factor (IGF-1) is primarily mediated by the growth hormone effect and produces IGF-1 in hepatocytes. It promotes growth and promotes the proliferation of all cells. It not only has a similar action effect to insulin, but also participates in synthesis and regulates cell growth and differentiation. Aging causes blood vessels to form in blood vessels, thereby regenerating the cells themselves. As a result, body fat is reduced to form a body without blood, muscle, nerve tissue and regenerate damaged cells.

The acetylhexapeptide-8 used in the present invention is called a Botox-like component, and it is a kind of Botox-like component, which is caused by a reaction such as movement or contraction of muscles caused by laughing or frown (Dynamic Wrinkle - It is possible. These muscle movements create wrinkles that reduce muscle contraction, which reduces wrinkle depth and prevents wrinkles. This function has been nicknamed Botox, because it plays a role similar to that of Botox. This is a new concept of functional wrinkle which is safe to the skin supplementing Botox problem. It has wrinkle improving effect when used for a long time and synergy effect when used with EGF. can see.

In the present invention, capper tripeptide-1 may be included as an active ingredient. Wrinkles are caused by lack of collagen and disorganized accumulation of skin cells. A substance called Copper tripeptide-1 is a substance that minimizes skin scars and stimulates skin regeneration to promote wound healing. It has been widely used as a wound healing promoter, but it is known to play an important role in the synthesis of collagen and elastin, which are the most important components of the dermal layer of the skin. This ingredient promotes normal skin regeneration and reduces scar tissue formation compared to other wound healing materials. It induces destruction of the super large collagen aggregate found in scars, and promotes the synthesis of common collagen and elastin. Capper tripeptide-1 has excellent ability to improve the treatment of skin wounds and is used for skin regeneration treatment such as laser regeneration, peeling or peeling, and also works well on skin damaged by ultraviolet rays.

The ginseng callus culture contained in the present invention: Callus is called a stem cell of a plant, and is a tissue having a strong split around the wound when a plant is wounded. Callus is highly active in physiological active substances such as anti-inflammation, antioxidant, anti-aging etc. than general plants because it is divided. Ginseng is a useful nutrient for the human body including saponin. It gives antioxidant, anti-aging and anti-inflammation effect to the skin and makes it more beautiful and healthy skin. This ginseng stem cell culture has high safety and biocompatibility, It is possible to enjoy a superior advantage more highly.

The plant extract fermentation liquid contained in the composition of the present invention is a substance obtained by fermenting Puerariae radix, horse mackerel, pomegranate, Omija, or sugar beet with Streptococcus thermophillus, and the above ingredients exert an excellent effect both inside and outside the skin cells do. It has been reported that rootlets and flowers are used for sweating, fever, fever, swelling of head and neck, acute otitis media and acute otitis media. Recently, It is known that isoflavones contained in purpura are effective for skin aging caused by ultraviolet rays. Horse riding is distributed in South Korea, the northeast of China in the riverside, and deep-forested horse riding is known to contain substances that act like women's estrogen. It also has anti-inflammatory and anti-convulsive properties and becomes a tonic for women's uterus just after delivery. It started with traditional therapies in India and now its efficacy has been clinically proven and emerging in the pharmaceutical industry. Pomegranate is rich in carbohydrates, amino acids, vitamins, and acids as well as rich potassium, glutinous and tannin components, and has effects of convergence, blood purification, and antioxidation. Omiza stimulates the protein synthesis of the cell and promotes the turnover of the cell. Gomisine A and Schizandrin in Omiza are known to have strong neurostabilizing and cell activating action, germ cell proliferation and promoting ovulation ovulation, and delaying the aging of the human body. Angelica helps to make the skin soft and smooth. Moreover, Angelica gigas is the third most frequently used herbal medicinal plant among 330 herbal medicine ingredients used in prescription of effective traditional commercial medicine 474. The liquid fermented by mixing the above plant components can be directly applied to the skin because isoflavones, turbulence, and the like are uniformly contained therein.

In the present invention, it is possible to use an emulsifying agent, which may contain an emulsifying agent, and is selected from the group consisting of glycol stearate, cetearyl alcohol, stearic acid, hydrogenated lecithin, cetearyl olivate, sorbitan olivate, A mixture of one or more of the group consisting of glyceryl stearate, cetyl palmitate, sorbitan palmitate, and sorbitan olivate may be used. In addition, it may contain a moisturizing agent in water, and it may contain an appropriate amount of butyleneglycol glycerin sodium hyaluronate, panthenol, betaglucan and the like. Skin protectants may also be included and it is preferred to use dipotassium glycyrrhizate adenosine allantoin niacinamide aloe vera leaf juice, octagonal fennel extract, or a mixture thereof. It is preferred to include an acidity adjusting agent, which is suitable as a triethanolamine chelating agent such as disodium and dithia.

The composition of the present invention may contain a viscosity modifier depending on the formulation, which is preferably xanthan gum. It may also contain components such as skin conditioners, moisture barrier agents, skin softening agents and the like, such as butyleneglycol dicaprylate / dicaprate, cetylhexanohexanoate, trimethylolpropane tricaprylate / Tricaprylate, hydrogenated coconut oil, trimethylpentanediol / adipic acid copolymer, dimethicone, tocopheryl acetate, and the like.

The cosmetic composition of the present invention is not particularly limited in its formulation and may be, for example, a cosmetic composition having a formulation of softening water, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic.

Example 1 : Spice preparation step

Sponges are washed with 0.1 M hydrochloric acid solution for 5 minutes and then heated in 30% (w / w) hydrogen peroxide for 1 hour. The speckle extracted from this is suspended in a 0.5 M hydrochloric acid solution for 1 day and then washed with phosphate buffered saline to neutralize the acidity. When this is dried at room temperature, spicule is completed.

Example 2 : Cosmetic composition production

20 to 30 parts by weight of a stem cell culture liquid extract and 10 to 20 parts by weight of a recombinant growth factor (EGF), cell growth factor (CGF), vascular endothelial growth factor (VEGF) (FGF), insulin-like growth factor (IGF-1) and acetylhexapeptide-8, copper tripeptide-1, ginseng callus culture solution and plant extract fermentation solution are mixed in the same amount) And 20 to 30 parts by weight of the mixture are mixed at room temperature for 24 to 48 hours for immersion. 5 to 10 parts by weight of a moisturizing agent and 5 to 10 parts by weight of a skin protective agent are added, and 5 to 10 parts by weight of disodium thiothiandate is added as a triethanolamine chelating agent as an acidity regulator. 5 to 10 parts by weight of xanthan gum and a carbomer, which are viscosity regulators, are pre-dissolved in the fermentation filtrate. 4 to 10 parts by weight of an emulsifier is added. 5 to 10 parts by weight each of ingredients such as a skin conditioner, a moisture-blocking agent, and a skin-softening agent are respectively added. Each component is weighed and divided into water phase and oil phase, heated at 70 to 80 ° C respectively, and dissolved in water. The oil phase is emulsified in the water phase for about 10 minutes. Thereafter, 1 to 5 parts by weight of a viscosity adjusting agent is added and mixed for 10 minutes. Cool the temperature to 30 占 폚. Finally, pre-immersed spu- cules are added and mixed well for 10 to 20 minutes.

Test Example 1

As shown in Fig. 1, the skin layer before and after applying the composition of the present invention to the skin was observed under a microscope. Collagen production was promoted after application of the composition, and skin layers were formed densely between the wrinkles, thereby confirming that wrinkles were improved.

Also, as shown in FIG. 2, it was confirmed that both skin tone, fine lines and deep wrinkles had excellent effects.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. There will be. The invention is not limited by these variations and modifications, but is limited only by the claims appended hereto.

Claims (9)

The sponges were washed with 0.1 M hydrochloric acid solution for 5 minutes and then heated in 30% (w / w) hydrogen peroxide for 1 hour. Spicules extracted from the sponges were suspended in 0.5 M hydrochloric acid solution for 1 day, Preparing a spicule which is washed with saline to neutralize the acidity and then dried at room temperature;
Human adipose stem cell culture (AAPE); Epithelial growth factor (EGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and insulin-like growth factor (IGF-1), which are cell growth factors (CGFs); Acetyl hexapeptide-8; Copper tripeptide-1; Ginseng callus culture; And a plant extract fermentation liquid to prepare a composition;
Immersing 10 to 20 parts by weight of the speckle in 20 to 30 parts by weight of the mixed composition for 24 to 48 hours;
Mixing the immersed spicule with an additive mixture in which a moisturizing agent, a skin protecting agent, a viscosity controlling agent, and an emulsifying agent are mixed, to prepare a cosmetic composition,
The additive mixture is prepared at 70-80 < 0 > C,
The method of producing a cosmetic composition for skin care according to claim 1, wherein the plant extract fermentation broth is fermented with Streptococcus thermophillus as a fermented product of Puerariae radix, horse riding, pomegranate,


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