KR102248733B1 - Functional cosmetic composition for improving skin wrinkles and elasticity - Google Patents

Abstract

The present invention relates to a functional cosmetic composition for reducing skin wrinkles and improving elasticity, and specifically, to a functional cosmetic composition for reducing skin wrinkles and improving elasticity, containing purified spicule, glyceryl glucoside, sodium hyaluronate, niacinamide arginine, caprylyl glycol, 1,2-hexanediol, and adenosine as active ingredients.

Description

Functional cosmetic composition for improving skin wrinkles and elasticity}

The following examples include purified spicule, glyceryl glucoside, sodium hyaluronate, niacinamide arginine, caprylyl glycol, 1,2-hexanediol and adenosine as active ingredients, skin wrinkles and elasticity It relates to a cosmetic composition technology for improvement.

As appearance is recognized as a measure of health and interest in skin health is increasing accordingly, interest in eco-friendly materials that are harmless to the human body and have a functional effect on the skin is increasing in the cosmetic industry. In particular, synthetic surfactants, pigments, antioxidants, and chemical preservatives contained in cosmetics are known to make the skin more dry or cause rash, inflammation, skin disease, and even skin cancer. There is a high need for cosmetics that use substances as main ingredients. In particular, research on natural-derived functional substances is in the spotlight in the field of functional cosmetics that focus on the secondary functional effects of cosmetics.

For example, spicule, a natural substance, is a natural substance known to promote skin metabolism, increase the absorption rate of active ingredients, promote skin regeneration, and induce normal keratinization. However, this natural spicule is a composite material having irregular molecular asymmetry (chirality) in shape and size, and has a problem in that stability is low, penetration amount, and absorption rate are low, so that it is conventionally used only as a filling material.

In addition, natural extracts and compounds are used as raw materials for functional cosmetics, but the absorption rate into the skin is not high in most cases. Above all, wrinkles, elasticity, moisturization, inflammation relief, soothing effect, whitening, etc. There is a difficulty in that the degree to which an actual user experiences a functional effect is insignificant.

Under this background, the present inventors made diligent efforts to solve the problems of conventional cosmetics composed of natural extracts and compounds as described above, and composed of natural substance-derived substances, resulting in high safety and excellent absorption rate, and a composite composition that leads to a distinct functional effect. The present invention was completed by studying the cosmetic composition of.

Korean Patent Application Publication No. 10-2016-0064410 Korean Patent Publication No. 10-1897401

The present invention contains purified spicule, glyceryl glucoside, sodium hyaluronate, niacinamide arginine, caprylyl glycol, 1,2-hexanediol and adenosine as active ingredients, improving skin wrinkles and elasticity To provide a cosmetic composition for.

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object is, including purified spicule, glyceryl glucoside, sodium hyaluronate, niacinamide arginine, caprylyl glycol, 1,2-hexanediol and adenosine as active ingredients, skin wrinkles and elasticity It can be achieved by the cosmetic composition for improvement.

Specifically, the cosmetic composition may further include hydrolyzed collagen, hydrolyzed elastin, and hydrolyzed hyaluronic acid as active ingredients.

Specifically, the cosmetic composition may further include cordyceps extract, sweet acacia flower extract, green tea extract, ginkgo biloba extract, peach tree extract and gasiogapi extract as active ingredients.

Specifically, the cosmetic composition is anastatica extract, Provence rose extract, Bambusa vulgaris extract, Stucky extract, Byeongpung extract, Aloe extract, Rosemary extract, Yakmo wheat extract, Orange flower oil, Damask rose flower oil, Violet flower extract , Brier flower fruit extract and purslane extract may be further included as active ingredients.

Specifically, the cosmetic composition is gold, cordycepin, vitamin E, acetylhexapeptide-8, RH-oligopeptide-1, RH-polypeptide-1, galloyltripeptide-35, nicotinoyl octapeptide- 9, RH-polypeptide-3, copper tripeptide-1, and palmitoyl-tripeptide-3-amide may be further included as active ingredients.

The cosmetic composition according to the present invention exhibits excellent improvement effects in the depth of thick wrinkles and fine lines due to skin aging, the degree of skin sagging, skin elasticity and density, and can be used as a functional material for skin barrier recovery and skin improvement cosmetics.

However, the effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a view showing changes in eyebrow wrinkles before and after a procedure according to an embodiment.
2 to 4 are views showing changes in nasolabial folds before and after a procedure according to an embodiment.
5 is a view showing the effect of maintaining facial wrinkles after a procedure according to an embodiment.
6 to 9 are diagrams showing changes in the degree of facial skin sagging before and after a procedure according to an embodiment.
10 and 11 are diagrams showing changes in skin elasticity and skin density before and after a procedure according to an embodiment.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, since various changes may be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It is to be understood that all changes, equivalents, or substitutes to the embodiments are included in the scope of the rights.

Specific structural or functional descriptions of the embodiments are disclosed for the purpose of illustration only, and may be changed and implemented in various forms. Accordingly, the embodiments are not limited to a specific disclosure form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical idea.

Although terms such as first or second may be used to describe various components, these terms should be interpreted only for the purpose of distinguishing one component from other components. For example, a first component may be referred to as a second component, and similarly, a second component may be referred to as a first component.

When a component is referred to as being "connected" to another component, it is to be understood that it may be directly connected or connected to the other component, but other components may exist in the middle.

The terms used in the examples are used for illustrative purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly indicates otherwise. In the present specification, terms such as "comprise" or "have" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or numbers, steps, actions, components, parts, or combinations thereof does not preclude in advance.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the embodiment belongs. Terms as defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in the present application. Does not.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms different from each other, and only these embodiments make the disclosure of the present invention complete, and common knowledge in the technical field to which the present invention belongs. It is provided to completely inform the scope of the invention to the possessor, and the invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein including technical or scientific terms are the same as those generally understood by one of ordinary skill in the art to which the present invention belongs. It has meaning. Terms as defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless explicitly defined in the embodiments of the present invention, an ideal or excessively formal meaning Is not interpreted as.

The shapes, sizes, ratios, angles, numbers, etc. disclosed in the drawings for explaining the embodiments of the present invention are exemplary, and thus the present invention is not limited to the illustrated matters. In addition, in describing the present invention, when it is determined that a detailed description of a related known technology may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted. When'include','have','consists of' and the like mentioned in the present specification are used, other parts may be added unless'only' is used. In the case of expressing the constituent elements in the singular, it includes the case of including the plural unless specifically stated otherwise.

In interpreting the constituent elements, it is interpreted as including an error range even if there is no explicit description.

The size and thickness of each component shown in the drawings are illustrated for convenience of description, and the present invention is not necessarily limited to the size and thickness of the illustrated component.

Each of the features of the various embodiments of the present invention may be partially or entirely combined or combined with each other, and as a person skilled in the art can fully understand, technically various interlocking and driving are possible, and each of the embodiments may be implemented independently of each other, It may be possible to do it together in a related relationship.

One aspect of the present invention is a skin comprising purified spicule, glyceryl glucoside, sodium hyaluronate, niacinamide arginine, caprylyl glycol, 1,2-hexanediol and adenosine as active ingredients It provides a cosmetic composition for improving wrinkles and elasticity.

In the present invention, spicules are derived from spicules of Sclerospongia, and refer to a natural composite material consisting of a jelly-shaped collagen matrix skeleton.

Specifically, the cosmetic composition is based on 100% by weight of the cosmetic composition, purified spicules 1 to 3% by weight, glyceryl glucoside 1 to 1.5% by weight, sodium hyaluronate 2 to 3% by weight, niacinamide 1 to It may contain 2% by weight, 1 to 2% by weight of arginine, and 2 to 2.5% by weight of carylyl glycol.

In one embodiment, the cosmetic composition may further include hydrolyzed collagen, hydrolyzed elastin, and hydrolyzed hyaluronic acid as active ingredients.

Specifically, the cosmetic composition may include 2.5 to 3.5% by weight of hydrolyzed collagen, 3 to 4% by weight of hydrolyzed elastin, and 3 to 4% by weight of hydrolyzed hyaluronic acid.

In one embodiment, the cosmetic composition may further include cordyceps sinensis extract, sweet acacia flower extract, green tea extract, ginkgo biloba extract, pica tree extract, and gasiogapi extract as active ingredients. The cosmetic composition of the present invention may improve the effect of the functional cosmetic composition for improving wrinkles and elasticity reduction due to skin aging by additionally including such a natural plant extract.

In one embodiment, the cosmetic composition is Anastatica extract, Provence rose extract, Bambusa bulgaris extract, Stucki extract, Byeongpung extract, Aloe extract, Rosemary extract, Yakushi extract, Orange flower oil, Damask rose flower oil, Violet flower extract, brier flower extract, and purslane extract may be further included as active ingredients.

In one embodiment, the cosmetic composition is gold, cordycepin, vitamin E, acetylhexapeptide-8, RH-oligopeptide-1, RH-polypeptide-1, galloyltripeptide-35, nicotinoyl Octapeptide-9, RH-polypeptide-3, copper tripeptide-1, and palmitoyl-tripeptide-3-amide may be further included as active ingredients.

In one embodiment, the cosmetic composition may be characterized in that for improving skin aging.

The cosmetic composition is one or more selected from the group consisting of softening lotion, nutritional lotion, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. It can be formulated.

The cosmetic composition may be formulated by a conventional method in the art. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the contents disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions. , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial lotion and body lotion; Creams such as nourishing cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Alternatively, it may be formulated with a detergent such as cleansing foam, soap, and body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In each formulation, the cosmetic composition may be appropriately blended with other components within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use, in addition to the essential components.

The cosmetic composition may contain a generally acceptable carrier, and for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or these Mixtures can be used,

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further include a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil can be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan can be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. Can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Hereinafter, the configuration of the present invention and effects thereof will be described in more detail through specific examples and comparative examples. However, the present examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

Preparation Example 1: Preparation of cosmetic composition

A cosmetic composition for improving skin wrinkles, whitening and moisturizing was prepared by mixing the components shown in Table 1 below. Depending on the content of each component, the prepared composition was named Examples 1 and 2 (T1 and T2, respectively), respectively.

Ingredients
(Based on 100 parts by weight of cosmetic composition) Example 1 (T1) Example 2 (T2) Refined spicule 2.00 2.00 Glyceryl glucoside 1.25 1.25 Sodium hyaluronate 2.50 2.50 Niacinamide 1.50 1.50 Arginine 0.50 0.50 Caprylyl Glycol 2.25 2.25 1,2-hexanediol 2.50 2.50 Adenosine 1.00 1.00 Hydrolyzed Collagen 3.00 3.00 Hydrolyzed Elastin 3.50 3.50 Hydrolyzed Hyaluronic Acid 3.50 3.50 Cordyceps sinensis extract 0.00 3.00 Sweet acacia flower extract 0.00 3.00 Green tea extract 0.00 3.00 Ginkgo biloba extract 0.00 3.00 Pica tree extract 0.00 3.00 Gasiogapi extract 0.00 3.00 Purified water Balance Balance

In addition, in the cosmetic composition of Example 2, in order to improve the wrinkle and elasticity improvement effect and improve the absorption rate of the cosmetic composition, Anastatica extract, Provence rose extract, Bambusa vulgaris extract, Stucki extract, Byeongpung extract, Aloe extract, rosemary Extract, Yakushi extract, orange flower oil, damask rose flower oil, violet flower extract, brier flower extract, purslane extract, cordycepin, vitamin E, acetylhexapeptide-8, RH-oligopeptide-1, RH-poly Peptide-1, galloyl tripeptide-35, nicotinoyl octapeptide-9, RH-polypeptide-3, copper tripeptide-1 and palmitoyl-tripeptide-3-amide at a concentration of 1% by weight, respectively. Was added, and gold was added at a concentration of 30 ppm.

For each plant extract, the flowers, stems, etc. of the obtained plant were dried at 80° C. for 10 hours using a drying oven. Dried 10g was added to 250 mL of 70% ethanol, extracted under reflux for 8 hours at 100°C, and the filtrate was concentrated with a vacuum concentrator to obtain each plant extract.

Preparation Example 2: Preparation of cream formulation

A cream formulation was prepared using the cosmetic compositions of Examples 1 and 2.

The cosmetic compositions of Examples 1 and 2 used in this experimental example were prepared as o/w creams. At this time, distilled water (sterile water) is used in order not to act as a variable as an aqueous raw material (water base, W/B), and the oil base (O/B) used to make a cream formulation is derived from grape seed oil and natural origin. O/w type emulsifier and cetyl alcohol, which are water-soluble emulsifiers, were used in common, respectively. In addition, a homomixer (WOWON, Korea) was used for cooling each formulation, and the containers according to each test group were sterilized with an aqueous 70% ethanol solution with the highest sterilizing power, and then the contents were filled.

Specifically, the cream to which the cosmetic composition was added was heated to 75° C. after the W phase corresponding to the aqueous phase dissolving bath was added, and the O phase was added to the oil dissolving bath, and then heated to 75° C. to completely dissolve the contents. After heating, the heated and dissolved O phase was slowly added to the W phase transferred to the main mixer, stirred in an o/w type emulsion control, and then stirred in a homomixer until the formulation was cooled to 55°C. It emulsified at (3,000 rpm) (about 7-8 minutes).

Meanwhile, the additives corresponding to the aqueous phase dissolving tank are slowly added in the order of composition, heated to 55°C to completely dissolve, and then slowly added to the main mixer cooled to 55°C, using a homomixer (3,000 rpm) until the formulation is cooled to 30°C. The cream was prepared by stirring for about 5 minutes. The finished cream was filled in an ethanol-sterilized container and then stabilized at room temperature (23±2°C) for 24 hours.

Experimental Example 1: Clinical test on whether cosmetic composition improves skin wrinkles and elasticity

1-1. Guidance on how to select and apply research subjects

In accordance with the guidelines for human application testing and efficacy testing of cosmetics (Korea Food and Drug Administration, 2015), this study was approved by the Institutional Bioethics Committee (70005239-AB-N-01-2018-KIDS-AHI032-HR- 01) The clinical trial procedure was carried out.

Research subjects were selected as the final 20 women in their 30s to 60s residing in the metropolitan area, and then the subjects were 6 subjects in the C group as the control group, 7 subjects in the T1 group as the test group 1 (Example 1), and the second test group Example 2) T2 group was divided into 3 test groups of 7 patients, and the clinical trial was conducted for about 4 weeks.

Two creams (100 mL) that are used for about 4 weeks for the study subject, and the same cleanser (Cleasing foam, VonEstis, Korea) and sun cream (Sun cream, VonEstis, Korea) to secure the homogeneity of the subject's skin application environment during the test. ) Were supplied together, and the study subjects were guided to apply twice a day, every morning and evening while directly storing at room temperature according to the method in Table 2.

Specifically, after washing your face with the cleanser provided by the researcher, take 1 tablespoon (about 1 g) of cream with a spatula provided with the spatula and apply it three times by tapping it for absorption. However, after applying the cream to the test product in the morning, be sure to apply the provided sun cream. During the test period, the use of functional cosmetics such as eye cream, whitening cream, and anti-aging cream that may affect the test result, as well as exfoliating agents and body moisturizers, other than the products provided, was prohibited. (Table 2)

In addition, at the beginning of the clinical trial period, it was sufficiently explained that it was different from the usual cosmetics and that the amount of use was suddenly decreased, and that the skin may be uncomfortable, such as a dry or prickly sensation.In the case of a shortage of the product during the test period, it was supplied immediately, erythema, swelling, and itchiness. In the event of adverse side effects, such as, it was guided so that it can be stopped immediately.

Considerations Regulation Frequency of use 2 Times/ 1 day Periodicity of use Morning and night / day Quantity of use Apply on each 3 spoons / once Part of use On facial and neck skin Period of use For 4 weeks

1-2. Evaluation item

In the clinical trial conducted for this study, the visual evaluation and scientific measurement of skin changes before and after the clinical trial were performed using a skin measuring device. For visual evaluation, the front and side of the facial skin and the left forearm skin were photographed using a camera before and after the clinical trial, and the changes were observed. The depth of thick wrinkles, the depth of fine wrinkles, the degree of sagging of the skin, the elasticity of the skin, and the density of the skin were observed. These items were measured for each area, and after a total of 5 measurements for each item for each measurement area, the average value was taken as the final measurement value and analyzed.

As an image evaluation, a digital camera (Galaxy note 8, Samsung, Korea) was used twice before and after the clinical trial to visually evaluate changes in the skin condition of the study subjects before and after the clinical trial. The front, side and left forearm were photographed. Immediately before entering the skin measurement room, when the test product was supplied, one person of the same photographer took an image at a designated shooting location where the same cleanser was supplied and the illumination and location were adjusted equally.

On the other hand, there is a limit to accurately observing changes in skin conditions before and after the clinical trial of this study subject only with the naked eye, so for a more scientific and objective measurement, skin changes before and after the clinical trial were evaluated using a skin measurement device. To this end, when the subject's skin was measured, the environment of the skin measurement room was set to an optimal constant temperature and humidity condition of 22±2℃ and a relative humidity of 50±5% to provide an accurate skin measurement environment. Was measured after being allowed to rest for 30 minutes or more while lying down immediately.

In addition, in order to evaluate the maintenance effect of each evaluation item according to each test group, the test product according to each test group was used for 4 weeks and the first measurement was taken immediately after stopping, and then the test product and all functional cosmetics were tested for 24 hours on the face area. After stopping the use, the facial area was measured a second time.

On the other hand, the measurement site is the forehead, the central point that connects the center between the two brows and the hairline vertically, the lower eyelid, the lower eyelid that is vertically below the pupil, and the 3 cm area next to the tail of both eyes, the point directly below the outer side of the eye. , The right cheek at the point where the part that connects vertically from the pupil and the part that connects horizontally from the tip of the nose to the ear meets, and both nasolabial parts in the diagonal direction from the nose to the end of the lips, and the tail of the mouth are selected, respectively, and tested. After measuring the skin condition immediately before and after 4 weeks of use, the effect on facial skin changes was evaluated.

However, the same test person in charge measured the corresponding part of all study subjects, and for the reproducibility of the measurement, the same part was measured and recorded by overlapping the image of the measured part taken at the time of the initial measurement.

The device used for skin measurement was Antera 3D (Miravex, Ireland) to measure the wrinkle depth of each part of the facial skin and the lifting improvement effect of skin sagging, and the Dermal torque meter (DTM 310) was used to evaluate the skin elasticity. , Dia-stron Ltd., UK), and DUB-skin Scanner (Taberna pro medicum, Luneburg, Germany), respectively, were applied to evaluate skin density.

1-3. Statistical analysis

The collected data of this study were analyzed using SPSS Win 24.0 program. One-way ANOVA was performed to verify the homogeneity of the skin condition of the study subjects, and paired samples t- test was performed to determine changes in skin before and after the clinical trial between each test group. However, with the significance level () 0.05, statistical significance was confirmed through the calculated p-value of each item.

Experimental Example 2: Confirmation of changes in wrinkles and elasticity of facial skin

2-1. Changes in the depth of thick wrinkles on the facial skin

Table 3 and Figs. 1 to 4 are the results of analyzing the change in the depth of the thick wrinkles for each part of the facial skin before and after the clinical test of the test product applied according to the products of each test group applied to the facial skin by the subject. At this time, the measured value was a wrinkles large (3 mm filter) value representing the wrinkles of the skin using the indentation index, which is a measurement variable, and the measurement unit is mm. Compared to before the test, the decrease in the measured value means that there is an effect of improving thick wrinkles.

In the case of the control group, the depth of the thick wrinkles on the forehead increased by about 4.07% from 19.91 before the test to 20.72 after the test, while in the case of the test group 1 using the cream of Example 1, it was about 19.87 before the test to 18.93 after the test. It was significantly decreased by 4.73% (p<.01). In contrast, the depth of the thick wrinkles on the forehead of the test group 2 using the cream of Example 2 significantly decreased by about 20.48% from 20.25 before the test to 16.10 after the test (p<.001).

The depth of the left thick wrinkle of the nasolabial fold significantly increased by 18.01% (p<.05) from 27.60 before the test to 32.57 after the test in the control group, whereas in the case of the test group 1, it was about 2.43% significant from 27.56 before the test to 26.89 after the test. Decreased significantly (p<.05). In contrast, in the case of test group 2, it significantly decreased from 30.15 before the test to 24.94 after the test to about 17.29% (p<.01).

In addition, in the depth of the right side of the nasolabial fold, the control group showed a significant increase of about 15.08% from 34.86 before the test to 40.12 after the test (p<.001), whereas the test group 1 showed a significant increase from 30.31 before the test to 29.60 after the test. There was a trend of 2.32% decrease. In contrast, in the case of test group 2, about 18.19% significantly decreased from 34.50 before the test to 28.22 after the test (p<.01).

In addition, the change was also evident in the visual evaluation, and in the case of the test group 2 in which the cream of Example 2 was applied, the deep nasolabial folds on the face before the test became lighter after 4 weeks of the test, as well as the diameter of the pores decreased. It was confirmed that the sagging was also reduced, and it was found that the depth of the thick wrinkles of the facial skin was improved (FIG. 3). In addition, it can be seen that the coarse nasolabial folds before the test not only decreased the depth of the nasolabial folds 4 weeks after the test, but also increased the elasticity of the skin surface itself (FIG. 4).

As described above, the width of change before and after the test for the depth of thick wrinkles in each part of the facial skin differs greatly depending on each test group.However, in the case of test group 1 using a functional cream, compared to the control group in which all of the wrinkles were deeper, the wrinkles were generally wrinkled. The depth of the test was reduced, and in particular, the test group 2 to which the cream of Example 2 was applied showed that the depth of the thick wrinkles by each part was significantly less than that of the other test groups.

As a result of the above, the creams of Examples 1 and 2 were found to have a positive effect on improving the forehead and thick wrinkles of the facial skin of women in their 40s to 50s. In particular, it was confirmed that it can be a material for wrinkle-improving cosmetics that lighten thick wrinkles.

Variable Group
(N) Measurement
(Mean±SD) t ₁ -t ₂ t p Before After Brow wrinkles C (6) 19.91±2.16 20.72±2.23 -.81±.86 -2.300 .070 T1 (7) 19.87±3.87 18.93±3.74 .94±.51 4.863 ^** .003 T2 (7) 20.25±3.21 16.10±3.53 4.15±1.16 9.444 ^*** .000 Nasolabial
fold Left C (6) 27.60±7.40 32.57±6.98 -4.97±3.09 -3.939 ^* .011 T1 (7) 27.56±5.02 26.89±4.94 .67±.60 2.924 ^* .027 T2 (7) 30.15±2.93 24.94±2.51 5.21±2.68 5.141 ^** .002 Right C (6) 34.86±8.44 40.12±8.33 -5.26±1.45 -8.855 ^*** .000 T1 (7) 30.31±7.42 29.60±7.14 .70±1.18 1.575 .166 T2 (7) 34.50±9.69 28.22±8.27 6.27±3.42 4.858 ^** .003

^* p<.05, ^** p<.01, ^*** p<.001

Abbreviation: t ₁ is the pre-clinical measurement; t ₂ is the measurement after the clinical trial; p is a valid value by pared samples t -test.

2-2. Improvement and maintenance effect of thick facial wrinkles after test

Tables 4 and 5 show the results of analyzing the effect of maintaining wrinkles 24 hours after stopping the application of the test product immediately after the clinical trial for each test group for the thick wrinkles of each facial skin part of the subject. Compared to before use, as the measured value decreases, it means that there is an effect of maintaining wrinkles 24 hours after the application of the test product is stopped.

In the case of the control group, the depth of the thick wrinkles on the forehead increased from 20.718 immediately after the test to 20.741 after 24 hours of stopping application in the control group, about 0.11%. In the case of the test group 1, from 18.928 immediately after the test to 18.934 after 24 hours of stopping application, about 0.03 % Showed a slight increase trend. On the contrary, the depth of the thick wrinkles of the forehead of the test group 2 showed a tendency to decrease slightly by about 0.03% from 16.105 immediately after the test to 16.100 even after 24 hours of stopping the application.

On the other hand, the left side of the nasolabial fold showed a tendency to increase by 0.99% from 32.565 immediately after the test in the control group to 32.888 after 24 hours of stopping application in the control group, and about 26.597 on the left side of the test group from 26.175 immediately after the test to 26.597 after 24 hours after stopping the application in the test group 1 Each showed a slight increase trend of 1.56%. On the other hand, in the case of test group 2, from 24.935 immediately after the test to 24.931 even after 24 hours of stopping the application, it showed a tendency to decrease slightly by about 0.02%.

In the case of the right side of the nasolabial fold, in the case of the control group, it was significantly increased from 40.116 immediately after the test to 40.574 after 24 hours of stopping the application by about 1.14% ((p<.05), and in the case of the test group 1, it increased from 29.604 immediately after the test to 24 hours after stopping the application. In the case of the test group 2, the trend was slightly increased by about 2.35% to 30.300. On the other hand, in the case of the test group 2, it showed a tendency to decrease slightly further from 28.221 immediately after the test to 28.019 even after 24 hours of stopping application.

As described above, after 24 hours of stopping the application of the test product immediately after the clinical trial, the width of the wrinkle retention effect varies greatly depending on each test group. As for the forehead and the thick wrinkles of both arms, as shown in Table 4, it was found that the depth of the wrinkles became lighter.

As a result of the above, the cream of Example 2 has a positive effect on the ability to further improve the condition for a certain period even if the application is stopped after the thick wrinkles of the facial skin of women in their 40s to 50s are improved. appear. Therefore, it was confirmed that it can be a material for wrinkle-improving cosmetics that not only lighten aging skin, especially thick wrinkles, but also maintain after improvement due to various factors.

Variable Group
(N) Measurement
(Mean±SD) t ₁ -t ₂ t p Before After Brow wrinkles C (6) 20.72±2.23 20.74±2.23 -.02±.14 -2.300 .070 T1 (7) 18.93±3.74 18.93±3.74 -.01±.02 -.646 .542 T2 (7) 16.10±3.53 16.10±3.53 .00±.02 .614 .562 Nasolabial
fold Left C (6) 32.57±6.98 32.89±7.04 -.32±.51 -1.554 .181 T1 (7) 26.89±4.94 27.31±4.85 -.42±.59 -1.900 .106 T2 (7) 24.94±2.51 24.93±2.79 .00±.42 .024 .981 Right C (6) 40.12±8.33 40.57±8.58 -.46±.42 -2.654 ^* .045 T1 (7) 29.60±7.14 30.30±7.24 -.70±.83 -2.224 .068 T2 (7) 28.22±8.27 28.02±8.17 .20±.32 1.676 .145

*p<.05

2-3. Changes in skin sagging degree and elasticity

According to the products of each test group applied to the facial skin of the subject, the results of analyzing the improvement of lifting by measuring the level of skin depressoins by each part of the facial skin before and after the clinical trial are shown in Table 5 and FIGS. Is the same as The measurement value analyzed the depressions large value representing the depressed volume of the skin, and the measurement unit was mm ³ . Compared to before use of the test substance, the lower the measured value, the better the lifting under the eyes.

In the degree of sagging under the eyes (eye bag), in the case of the control group, about 26.06% significantly increased from 3.36 before the test to 4.24 after the test (p<.05). On the other hand, in the case of test group 1, it significantly decreased by about 6.32% from 3.48 before the test to 3.26 after the test (p<.01). In contrast, the degree of sagging under the eyes (eye bag) in the test group 2 significantly decreased by 54.94% from 3.92 before the test to 1.77 after the test (p<.001).

In addition, in the case of the control group, the degree of sagging of the mouth tail showed a tendency to increase by about 10.88% from 7.57 before the test to 8.39 after the test, whereas in the case of the test group 1, it significantly decreased by about 0.14% from 7.33 before the test to 7.23 after the test (p<. 05). In addition, in the case of test group 2, it significantly decreased from 7.90 before the test to 3.57 after the test to about 54.74% (p<.01).

The change was also evident in the visual evaluation, and in the case of the test group 2 in which the cream of Example 2 was applied, it was difficult to find the tail of the mouth drooping after the test so that the tail of the mouth was drooping so that the nasolabial folds before the test were also connected. The degree of improvement has been improved, and in addition, it can be seen that the jawline before the test is clearly revealed after the test (FIG. 7). In addition, in the clinical subjects of the test group 2, where the sagging under the eyes (eye bag) was noticeable before the test, not only the sagging phenomenon under the eyes (eye bag) was remarkably improved after the test, but also the wrinkles under the eyes were noticeably lightened, and the degree of sagging of the skin was significantly reduced. It can be seen that there is a marked improvement (Figs. 8 and 9).

As described above, the extent of change before and after the test for the degree of skin sagging by each part of the facial skin was significantly different for each test group, but the degree of sagging of the skin under the eyes (eye bag) and the tail of the mouth were both more severe in the test group 1 than in the control group. In general, the degree of sagging of the skin was decreased, and the degree of sagging of the skin under the eyes (eye bag) and the tail of the mouth was significantly decreased in the test group 2 compared to the other test groups.

As a result of the above, the creams of Examples 1 and 2 appeared to have a very positive effect on improving the sagging under the eyes (eye bag) and the sagging of the mouth of the facial skin of women in their 40s to 50s. It was confirmed that the skin, in particular, can be a material for elasticity improvement cosmetics that improves skin sagging and sagging caused by sagging thin areas of the facial skin due to the loss of the power of the skin.

Variable Group Measurement
(Mean±SD) t ₁ -t ₂ t p Before After Eye bag C (n=6) 3.36±1.60 4.24±2.13 -.88±.66 -3.238 ^* .023 T1 (n=7) 3.48±1.64 3.26±1.58 .22±.15 3.952 ^** .008 T2 (n=7) 3.92±.92 1.77±.98 2.15±.62 9.193 ^*** .000 Oral angle C (n=6) 7.57±1.54 8.39±2.34 -.82±1.41 -1.426 .213 T1 (n=7) 7.33±1.91 7.23±1.87 .01±.09 2.989 ^* .024 T2 (n=7) 7.90±2.61 3.57±1.62 4.32±1.94 5.892 ^** .001

^* p<.05, ^** p<.01, ^*** p<.001

2-4. Changes in skin elasticity and skin density

According to the products of each test group applied to the facial skin by the subject of this study, the results of analyzing the degree of skin elasticity and the improvement of the density by measuring the elasticity and density values of the facial skin before and after the clinical trial are shown in Table 6 and FIG. Same as 11.

The analysis range of skin elasticity was limited to 3.0 mm of skin, and to measure this, a guard ring having a ring gap of 3 mm that transmits rotational force to the dermal layer was attached, and the Ur/Ue value indicating skin elasticity was used for analysis . In addition, the analysis range of skin density was limited to the upper part of the subcutaneous fat layer just below the epidermis, and the measurement unit was density. Both measured values mean that the skin elasticity and density improved as the measured value increased compared to that before the test.

In the case of the control group, the skin elasticity significantly decreased by about 11.06% from 0.38 before the test to 0.34 after the test (p<.01), whereas in the case of the test group 1, there was no change from 0.38 before the test to 0.38 after the test. On the contrary, the skin elasticity of the test group 2 significantly increased by about 22.26% from 0.38 before the test to 0.46 after the test (p<.001).

In addition, in the case of the control group, the skin density decreased significantly (p<.05) by about 13.05% from 24.66 before the test to 21.44 after the test (p<.05), while the test group 1 significantly increased by about 1.69% from 24.99 before the test to 25.41 after the test. (p<.05). In contrast, in the case of test group 2, the skin density increased significantly from 24.59 before the test to 38.21 after the test, about 55.36% (p<.001).

In the case of the test group 2, the size and sagging of the cheek pores before the test were severe, but after the test, not only the size and sagging of the pores, but also the relief of thick wrinkles and the smoothness of the skin were changed at a glance. It can be seen that the elasticity of the skin and the density of the dermis are improved (FIG. 11).

As described above, in the range of changes before and after the test for the skin elasticity of each part of the facial skin, in the case of the test group 1, the skin density was increased compared to the control group in which both the skin elasticity and the skin density were significantly more severe. In addition, the test group 2 showed a marked increase in skin elasticity and skin density compared to other test groups.

As a result of the above, the creams of Examples 1 and 2 were found to have a very positive effect on improving skin elasticity and skin density of women in their 40s to 50s. It was confirmed that it can be a material for elasticity improvement cosmetics that improves the loosening of skin tissue.

Variable
(value) Group Measurement
(Mean±SD) t ₁ -t ₂ t p Before After Skin
elasticity
(Ur/Ue) C (n=6) .38±.03 .34±,04 .04±.002 4.776 ^** .005 T1 (n=7) .38±.01 .38±.01 .00±.01 -1.441 .200 T2 (n=7) .38±.02 .46±.03 -.08±.03 -8.895 ^*** .000 Skin
density
(density) C (n=6) 24.66±2.78 21.44±4.60 3.22±2.94 2.680 ^* .044 T1 (n=7) 24.99±2.94 25.41±3.06 -.42±.37 -2.984 ^* .025 T2 (n=7) 24.59±1.97 38.21±2.16 -13.61±1.60 -22.487 ^*** .000

^* p<.05, ^** p<.01, ^*** p<.001

Although the embodiments of the present invention have been described in more detail with reference to the accompanying drawings, the present invention is not necessarily limited to these embodiments, and various modifications may be made without departing from the spirit of the present invention. . Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain the technical idea, and the scope of the technical idea of the present invention is not limited by these embodiments. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects. The scope of protection of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Therefore, other implementations, other embodiments, and claims and equivalents fall within the scope of the following claims.

Claims (3)

Purified spicules, glyceryl glucoside, sodium hyaluronate, niacinamide, arginine, caprylyl glycol, 1,2-hexanediol, adenosine, hydrolyzed collagen, hydrolyzed elastin, hydride Rolled hyaluronic acid, Cordyceps sinensis extract, Sweet acacia flower extract, green tea extract, ginkgo biloba extract, peach tree extract, gasoline extract, anastatica extract, provence rose extract, bambusa bulgaris extract, stucky extract, folding screen Extract, aloe extract, rosemary extract, yam extract, orange flower oil, damask rose flower oil, violet flower extract, brier flower extract, purslane extract, gold, cordycepin, vitamin E, acetylhexapeptide-8, RH-oligo Peptide-1, RH-polypeptide-1, galloyltripeptide-35, nicotinoyloctapeptide-9, RH-polypeptide-3, copper tripeptide-1 and palmitoyl-tripeptide-3-amide As a cosmetic composition for improving skin wrinkles and elasticity, comprising as an active ingredient,
The cosmetic composition is based on the total cosmetic composition, purified spicules 1 to 3% by weight, glyceryl glucoside 1 to 1.5% by weight, sodium hyaluronate 2 to 3% by weight, niacinamide 1 to 2% by weight, Al Possession 1 to 2% by weight, caprylyl glycol 2 to 2.5% by weight, 1,2-hexanediol 2.5% by weight, adenosine 1% by weight, hydrolyzed collagen 2.5 to 3.5% by weight, hydrolyzed elastin 3 to 4% by weight, 3 to 4% by weight of hydrolyzed hyaluronic acid, 3% by weight of Cordyceps sinensis extract, 3% by weight of sweet acacia flower extract, 3% by weight of green tea extract, 3% by weight of ginkgo biloba extract, Extract 3% by weight, Gasiogapi extract 3% by weight, Anastatica extract 1% by weight, Provence rose extract 1% by weight, Bambusa vulgaris extract 1% by weight, Stucky extract 1% by weight, Byeongpung extract 1% by weight, Aloe extract 1 Wt%, rosemary extract 1% by weight, yakmomile extract 1% by weight, orange flower oil 1% by weight, damask rose flower oil 1% by weight, violet flower extract 1% by weight, brier flower extract 1% by weight, purslane extract 1% by weight , Cordycepin 1% by weight, vitamin E 1% by weight, acetylhexapeptide-8 1% by weight, RH-oligopeptide-1 1% by weight, RH-polypeptide-1 1% by weight, galloyl tripeptide-35 1% by weight, nicotinoyloctapeptide-9 1% by weight, RH-polypeptide-3 1% by weight, copper tripeptide-1 1% by weight, palmitoyl-tripeptide-3-amide 1% by weight and gold 30 ppm The cosmetic composition for improving skin wrinkles and elasticity, including in a concentration.

delete delete

Images