CN115006322A

CN115006322A - Formula of instant and long-acting multifunctional eye mask and preparation method thereof

Info

Publication number: CN115006322A
Application number: CN202210633927.3A
Authority: CN
Inventors: 裴运林; 聂艳峰; 王娟; 郭朝万; 蒲艳; 胡露
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-06-06
Filing date: 2022-06-06
Publication date: 2022-09-06
Anticipated expiration: 2042-06-06
Also published as: CN115006322B

Abstract

The application relates to the field of cosmetics, and relates to a multi-effect eye mask with instant and long-acting effects, which comprises the following components: EX-distillers meal extract MR, schizophyllan-G1, nicotinamide, erythritol; a second component: haloxyyl PH MBAL, BioGenic CellLite-220, moistHield HA, all human ^TM Collagen, SENS' FLOWER SD-SC, NaturalSuperM @ 2-alpha GG-40, and XEP-018 conopeptide. Immediate efficacy: the glossiness of the skin under the eyes tends to be improved; the skin under the eyes tends to turn white; the canthus and infraocular wrinkles have a tendency to improve; a reduction in pouch volume; the water content of the stratum corneum of the skin under the eyes tends to be improved; long-acting efficacy: the glossiness of the skin under the eyes tends to be improved; the skin under the eyes tends to turn white; the canthus and infraocular wrinkles have a tendency to improve; there is a reduction in pouch volume.

Description

Formula of instant and long-acting multifunctional eye mask and preparation method thereof

Technical Field

The application relates to the technical field of cosmetics, in particular to a formula of a multifunctional eye mask with instant and long-acting effects and a preparation method thereof.

Background

Eye parts often have the conditions of edema, staying up all night, dark eye circles, eye bags and the like, and have the problems of wrinkles, glossiness, water content loss of skin cuticle and the like.

Therefore, it is required to supplement water in a short time, relieve fatigue, rapidly reduce edema and dark circles, and improve wrinkles, glossiness, water content loss of skin stratum corneum, etc. for a long time.

However, there is a lack in the art of eye mask products that are both immediate and long-lasting, yet are multi-functional.

Disclosure of Invention

The embodiment of the application aims to provide a formula of a multifunctional eye mask with instant and long-acting effects and a preparation method thereof.

In a first aspect, the present application provides a formulation for a multi-effect ophthalmic membrane having both immediate and long-lasting effects, comprising a first component, a second component and water;

the first component comprises:

EX-distillers meal extract MR, schizophyllan-G1, nicotinamide, erythritol;

the second component comprises:

haloxyyl PH MBAL, BioGenic CellLite-220, moistHield HA, all human ^TM Collagen, SENS' FLOWER SD-SC, NaturalSuperM @ 2-alpha GG-40, and XEP-018 conotoxin.

The present application provides a multifunctional eye mask with both immediate and long-lasting effects, which can achieve the following immediate effects:

the glossiness of the skin under the eyes is improved after the eye mask is used; the skin under the eyes tends to turn white; the canthus and infraocular wrinkles have a tendency to improve; a reduction in pouch volume; the water content of the stratum corneum of the skin under the eyes tends to be improved;

meanwhile, the following long-acting effects can be realized:

the glossiness of the skin under the eyes is improved after the eye mask is used; the skin under the eyes tends to turn white; the canthus and infraocular wrinkles have a tendency to improve; a reduction in pouch volume; there is a tendency for the water content of the stratum corneum of the skin under the eye to increase.

The instant and long-acting multifunctional eye mask is mild in formula and not easy to cause allergy.

In other embodiments of the present application, the first component further comprises:

glyceryl polyether-26, glycerin, low-molecular sodium hyaluronate and xanthan gum;

the second component further comprises:

1.3-butanediol, 1, 2-hexanediol, p-hydroxyacetophenone, hydrogenated castor oil, bergamot fruit oil, and Salvia miltiorrhiza oil.

In other embodiments of the present application, the eye mask comprises, in mass percent:

0.1% -0.5% EX-lees extract MR, 0.1% -0.5% schizophyllan-G1, 1% -5% nicotinamide, 2% -5% erythritol, 0.1% -2.5% Haloxyyl PH MBAL, 0.1% -0.5% BioGenic CellLite-220, 0.5% -2% MOISTSELD HA, 0.1% -2% of whole human source ^TM Collagen, 0.1% -0.5% SENS' FLOWER SD-SC, 0.5% -2% NaturalSuperM @ 2-alpha GG-40, 1% -5% XEP-018 conopeptide, 1% -5% glyceryl polyether-26, 1% -5% glycerin, 0.1% -0.3% low molecular sodium hyaluronate, 0.2% -0.4% xanthan gum, 2% -5% 1.3-butanediol, 0.3% -0.6% 1, 2-hexanediol, 0.3% -0.6% p-hydroxyacetophenone, 0.03% -0.05% hydrogenated castor oil, 0.001% -0.003% bergamot fruit oil, 0.001% -0.003% ondansu oil;

the balance being water.

In other embodiments of the present application, the eye mask with both immediate and long-lasting functions is a colorless transparent liquid;

in other embodiments of the present application, EX-distillers meal extract MR comprises the following components: rice fermentation product filtrate, butanediol, water, citric acid, sodium citrate, 1, 2-hexanediol and p-hydroxyacetophenone.

In other embodiments of the present application, NaturalSuperM @2- α GG-40 comprises the following components: glycerol glucoside, water, 1, 2-pentanediol.

In a second aspect, the present application provides a method for preparing an eye mask with both immediate and long-lasting effects,

adding water and the first component into a water phase pot, heating and preserving heat to obtain a first material;

adding the first material into an emulsifying pot, homogenizing and cooling, and then adding the second component.

In another embodiment of the present application, the step of adding water and the first component to the water phase pot, and raising the temperature and maintaining the temperature is to raise the temperature to 80 ℃ to 85 ℃ for maintaining the temperature.

In other embodiments of the present application, the first material is added to the emulsifying pot, homogenized and cooled to 44-46 ℃.

In other embodiments of the present application, the first material is added into the emulsifying pot, homogenized and cooled, and the homogenizing speed is 1000r/min to 2000 r/min.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is an immediate differential analysis of the ocular skin gloss values (

n＝32)；

FIG. 2 is a graph showing the instant difference analysis of the skin color under the eyes: (

n＝32)；

FIG. 3 is an immediate ocular analysis of differential skin haematochrome (EI) and Melanin (MI) values (

n＝32)；

FIG. 4 is a real-time analysis of the difference in volume between canthus and infraocular wrinkles (

n＝32)；

FIG. 5 is a real-time analysis of the difference between the area of canthus and infraocular wrinkles (

n＝32)；

FIG. 6 is a graph showing the instant difference between the area ratios of canthus and infraocular wrinkles (

n＝32)；

FIG. 7 is a graph of the instant differential analysis of pouch volume (A)

n＝32)；

FIG. 8 is a graph showing the differential analysis of the water content of stratum corneum of the skin immediately under the eyes: (

n＝32)；

FIG. 9 shows differential analysis of skin gloss values under extended-acting eyes: (

n＝30)；

FIG. 10 is a differential analysis of skin color under long-acting eyes: (

n＝30)；

FIG. 11 shows the differential analysis of the skin red pigment values and melanin values under the long-acting eyes: (

n＝32)；

FIG. 12 shows differential volume analysis of long-acting canthus and infraocular wrinkles (

n＝30)；

FIG. 13 shows the differential analysis of the area of the long-acting canthus and infraocular wrinkles (

n＝30)；

FIG. 14 is a differential analysis of the area fraction of long-acting canthus and infraocular wrinkles (

n＝30)；

FIG. 15 is a differential analysis of long-acting pouch volume (

n＝30)；

FIG. 16 is a differential analysis of water content of stratum corneum under prolonged eye conditions: (

n＝30)。

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the detailed description of the embodiments of the present application provided below is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Some embodiments of the present application provide a formulation for an ophthalmic membrane that provides both immediate and extended release functionality, comprising a first component, a second component, and water.

Further, the first component described above includes: EX-distillers meal extract MR, schizophyllan-G1, nicotinamide, erythritol.

Further, the EX-lees extract MR described above comprises the following components: rice fermentation product filtrate, butanediol, water, citric acid, sodium citrate, 1, 2-hexanediol and p-hydroxyacetophenone.

Further, the schizophyllan-G1 comprises the following components: butanediol, glucan, p-hydroxyacetophenone, 1, 2-hexanediol, sodium citrate, citric acid and water.

Further, in some embodiments herein, the nicotinamide described above can be obtained commercially, for example, by commercially purchasing nicotinamide Niacinamide PC from the lung corporation.

Further, in some embodiments herein, the erythritol described above can be obtained commercially, for example, by commercially purchasing erythritol Zerose from oxsnow ^TM Erythritol Standard Granular。

Further, the second component described above includes: haloxyyl PH MBAL, BioGenic CellLite-220, moistHield HA, all human ^TM Collagen, SENS' FLOWER SD-SC, NaturalSuperM @ 2-alpha GG-40, and XEP-018 conotoxin.

Further, the above-mentioned Haloxyyl PH MBAL comprises the following components: water, glycerol, steareth-20, N-hydroxysuccinimide, 5, 7-dihydroxyflavone, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, phenoxyethanol, potassium sorbate, citric acid and sodium citrate.

Further, the BioGenic CellLite-220 comprises the following components: seed extract of Coffea ARABICA (Coffea ARABICA), maltodextrin, cyclodextrin, and xanthan gum.

Further, the moitsthield HA described above comprises the following components: water, xanthan gum, butylene glycol, glucose, sodium hyaluronate, Chondrus CRISPUS (Chondrus CRISPUS) extract, hydroxypropyl trimethyl ammonium chloride hyaluronic acid, hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, hyaluronic acid, sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, potassium hyaluronate, 1, 2-hexanediol, and caprylyl glycol.

Further, the above-mentioned whole human source ^TM The collagen comprises the following components: water, glycerol, sodium dihydrogen phosphate, disodium hydrogen phosphate and soluble collagen.

Further, the SENS' FLOWER SD-SC comprises the following components: water, 1, 3-propanediol, and CROCUS SATIVUS (CROCUS SATIVUS) flower extract.

Further, the NaturalSuperM @ 2-alpha GG-40 comprises the following components: glycerol glucoside, water, 1, 2-pentanediol.

Further, the XEP-018 conopeptide comprises the following components: water, cysteine, serine, arginine/lysine polypeptide.

Further, in some embodiments of the present application, the first component further comprises: glyceryl polyether-26, glycerin, low-molecular-weight sodium hyaluronate and xanthan gum.

Further, in some embodiments of the present application, the second component further comprises: 1.3-butanediol, 1, 2-hexanediol, p-hydroxyacetophenone, hydrogenated castor oil, bergamot fruit oil, and Salvia miltiorrhiza oil.

Further, in some embodiments herein, the eye mask comprises, in mass percent:

0.1% -0.5% EX-lees extract MR, 0.1% -0.5% schizophyllan-G1, 1% -5% nicotinamide, 2% -5% erythritol, 0.1% -2.5% Haloxyyl PH MBAL, 0.1% -0.5% BioGenic CellLite-220, 0.5% -2% MOISTSELD HA, 0.1% -2% of whole human source ^TM Collagen, 0.1% -0.5% SENS' FLOWER SD-SC, 0.5% -2% NaturalSuperM @ 2-alpha GG-40, 1% -5% XEP-018 conopeptide, 1% -5% glyceryl polyether-26, 1% -5% glycerin, 0.1% -0.3% low molecular weight sodium hyaluronate, 0.2% -0.4% xanthan gum, 2% -5% 1.3-butanediol, 0.3% -0.6% 1, 2-hexanediol, 0.3% -0.6% p-hydroxyacetophenone, 0.03% -0.05% hydrogenated castor oil, 0.001% -0.003% bergamot fruit oil, 0.001% -0.003% ondansetron oil; the balance being water.

Further, in some embodiments of the present application, the eye mask that functions both immediately and permanently is a colorless transparent liquid.

The eye mask has the effects of being instant and long-acting, and is mild in formula.

Some embodiments of the present application provide a method for preparing an eye mask with both instant and long-lasting functions, which can be used to prepare an eye mask with both instant and long-lasting functions provided in any of the foregoing embodiments.

Further, the preparation method comprises the following steps: adding water and the first component into a water phase pot, heating and preserving heat to obtain a first material;

Further, adding water and the first component into a water phase pot, and in the step of heating and preserving heat, heating and preserving heat until the temperature is 80-85 ℃ for heat preservation.

Illustratively, the temperature rise and the heat preservation are carried out by raising the temperature to 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃ or 85 ℃.

Further, the first material is added into an emulsifying pot, and in the step of homogenizing and cooling, the temperature is reduced to 44-46 ℃.

Illustratively, the temperature is reduced to 44 ℃, 44.5 ℃, 45 ℃, 45.5 ℃ or 46 ℃.

Further, the first material is added into an emulsifying pot, homogenized and cooled, wherein the homogenizing speed is 1000 r/min-2000 r/min.

The homogenization speed is 1000r/min, 1200r/min, 1500r/min, 1800r/min or 1900 r/min.

The preparation method of the eye mask with instant and long-acting functions has the advantages of simple process, environmental protection and convenient use.

The features and properties of the present application are described in further detail below with reference to examples.

Example 1

An eye mask with both immediate and long-acting functions is provided, and the formula composition of the eye mask is shown in table 1:

TABLE 1

The preparation method comprises the following steps:

adding water and the first component into a water phase pot, heating to 80-85 ℃, and preserving heat to obtain a first material;

adding the first material into an emulsifying pot, homogenizing and cooling to 44-46 ℃, wherein the homogenizing speed is 1000-2000 r/min; the second component is then added.

Example 2

An eye mask with both immediate and long-acting functions is provided, and the formula composition of the eye mask is shown in table 2:

TABLE 2

The preparation method is the same as that of example 1.

Example 3

An eye mask with both immediate and long-lasting effects is provided, which has the formula composition as shown in table 3:

TABLE 3

The preparation method is the same as that of example 1.

The following tests were conducted to examine the efficacy of the cosmetics of examples 1 to 3, including immediate effect and long-lasting effect.

1. Test indexes

(1) Sub-ocular skin gloss;

(2) quantifying skin color by using three primary color stimulation values (L, a, b) determined by the International Commission on illumination, and calculating to obtain an individual type angle ITA value according to a formula to represent skin whiteness;

(3) the content of skin red pigment and melanin under eyes;

(4) the volume of wrinkles, the area of wrinkles and the ratio of the area of wrinkles in the canthus and under the eyes;

(5) the pouch volume;

(6) sub-ocular stratum corneum water content;

(7) subjective evaluation of subjects: the evaluation indexes of the product such as use effect, use feeling and use will.

2. Detecting an environment

The test environment is at 20.5-21.8 deg.c and 43.8-56.4% (RH) humidity, and is in accordance with the requirement of scheme design.

3. Results of instrumental testing

After face cleaning for 30min, acquiring a face image of a subject by using VISIA-CR, acquiring a wrinkle image of a measured area of the subject by using PRIMOS-CR, determining a wrinkle target analysis position, and obtaining eye corner and under eye wrinkle volume values, wrinkle areas, wrinkle area ratio and eye pouch volume by using analysis software; and CM825, GL200, MX18 and CM-700d were used to test skin stratum corneum moisture content, skin glossiness, skin haematochrome content, melanin content and skin color ITA ° values, all of which were baseline values (T0), and then product dispensing and methods of use were instructed. The subjects need to visit again 0.5 hour (T0.5h) after using the product and carry out the same index detection. Subjects were followed 1 time every 2 days for 28 consecutive days, followed by visits to the product for 7 days (D7) and 28 days (D28), and the same index tests were performed.

The test of the same subject is completed by the same instrument and the same tester. The test site should be kept consistent between the two test time points.

And counting the measured values of each test area, wherein the measured values comprise numerical values, mean values, standard deviations, standard errors, minimum values, median values, maximum values and the like.

If the test data is normal analysis, performing statistical analysis by adopting a t test method; and if the test data is in abnormal distribution, performing statistical analysis by adopting a rank sum test method.

The statistical method adopts two-tail test, and the test level alpha is 0.05.

Data statistics were analyzed for variability using SPSS25.0 software. When significant differences in results occurred, they were labeled with P < 0.05 and P < 0.01, respectively. P < 0.05 indicates statistically significant, and P < 0.01 indicates significant differences. If the numerical values of all indexes in the test area have statistical significance or significant difference, the product has the effects of improving wrinkles and moisturizing.

4. Immediate effect detection result

(one) under-eye skin gloss analysis

The results are shown in table 1, table 2 and figure 1:

table 1 sub-ocular skin gloss values descriptive statistics (n ═ 32)

TABLE 2 differential analysis of the skin gloss values under the eyes: (

n＝32)

Point in time	Mean ± standard deviation	P value (vs. T0)
			T0	3.68±1.91	/
T0.5h	5.80±2.08	<0.01

As can be seen from table 1, table 2 and fig. 1, the under-eye skin gloss values exhibited a rising trend after use of the product compared to the baseline value before use (T0). The under-eye skin glossiness value increased 57.58% after the product was used for 0.5 hour (T0.5h), and the under-eye skin glossiness value was higher than the baseline value after the application, which was analyzed by t-test and had a significant difference from the baseline value.

(second) under-eye skin color ITA-degree analysis

The results are shown in table 3, table 4 and fig. 2:

table 3 skin color under eye ITA ° value descriptive statistics (n ═ 32)

Point in time	Number of people	Mean value of	Standard deviation of	Standard error of	Minimum value	Maximum value	Median number
								T0	32	18.75	6.95	1.23	6.70	36.46	18.84
T0.5h	32	21.28	6.15	1.09	5.43	37.94	21.41

Table 4 under-eye skin color ITA ° value difference analysis (ii)

n＝32)

Point in time	Mean ± standard deviation	P value (vs. T0)
			T0	18.75±6.95	/
T0.5h	21.28±6.15	<0.01

As can be seen from tables 3, 4, and 2, the under-eye skin color ITA ° value exhibited a gradual upward trend after use of the product, increased by 13.51% after use of the product for 0.5 hours (T0.5h), was significant in difference from the baseline value T0 by T-test analysis, and increased after use of the product for 0.5 hours (T0.5h), as compared to the pre-use baseline value (T0).

Analysis of skin haematochrome (EI) value and Melanin (MI) value under eye

The results are shown in tables 5 and 6 and fig. 3:

table 5 ocular skin haematochrome and melanin values descriptive statistics (n ═ 32)

TABLE 6 analysis of the difference between the values of the skin red pigment and melanin under the eyes: (

n＝32)

As can be seen from tables 5, 6 and fig. 3, the under-eye skin red pigment and melanin values tended to decrease by 7.56% and 5.82% after 0.5 hours of use of the product, respectively, compared to the baseline value before use (T0). The difference of the product and a base line value T0 is significant through matching T test analysis, and the skin haematochrome value and the melanin value under the eyes are reduced after the product is used.

(IV) analysis of canthus and infraocular wrinkle volumes

The results are shown in tables 7 and 8 and fig. 4:

TABLE 7 Ocular Angle and infraocular wrinkle volume descriptive statistics (mm) ³ ,n＝32)

TABLE 8 analysis of volume difference (mm) of canthus and infraocular wrinkles ³ ,

n＝32)

As can be seen from tables 7 and 8 and fig. 4: both the canthus and sub-ocular wrinkle volume values exhibited a decreasing trend after use of the product compared to the pre-use baseline value (T0). Among them, the volume value of canthus wrinkles was decreased by 4.71% after 0.5 hours (T0.5h) of use of the product, as compared with the baseline value before use (T0). The analysis of the paired T test shows that the difference from the base line value T0 is significant, and the volume of canthus wrinkles is reduced after the product is used.

The sub-ocular wrinkle volume value decreased by 7.87% after 0.5 hours (T0.5) of use of the product compared to the pre-use baseline value (T0). The differences from the baseline value T0 were statistically significant as analyzed by the paired T-test.

(V) analysis of canthus and infraocular wrinkle area

The results are shown in tables 9 and 10 and fig. 5:

TABLE 9 Ocular Angle and infraocular wrinkle area descriptive statistics (mm) ² ,n＝32)

TABLE 10 analysis of the difference in area of canthus and infraocular wrinkles (mm) ² ,

n＝32)

As can be seen from tables 9 and 10 and fig. 5: both the canthus and infraocular wrinkle area values exhibited a downward trend after use of the product compared to the pre-use baseline value (T0).

Among them, the area value of canthus wrinkles was decreased by 9.23% after 0.5 hours using the product, compared with the baseline value before use (T0). The difference of the ratio T test and analysis and the base line value T0 is significant, and the wrinkle area of the canthus after the product is used is reduced.

The sub-ocular wrinkle area value decreased by 10.04% after 0.5 hours of use of the product compared to the pre-use baseline value (T0). The paired T-test analysis shows that the difference from the baseline value T0 is significant, and the wrinkle area under the eyes is reduced after the use.

(VI) analysis of area ratio of canthus and infraocular wrinkles

The results are shown in table 11, table 12 and fig. 6:

table 11 canthus and infraocular wrinkle area percentage descriptive statistics (%, n ═ 32)

Table 12 canthus and infraocular wrinkle area ratio differential analysis (%,

n＝32)

as can be seen from tables 11 and 12 and fig. 6: the canthus and infraocular wrinkle area ratios exhibited a reduced tendency after use of the product compared to the pre-use baseline value (T0)

Among them, the percentage of area occupied by canthus wrinkles decreased by 9.19% after 0.5 hour (t0.5h) from the baseline value before use (T0). The difference from the base line value T0 is significant through the rank sum test analysis, and the canthus wrinkle area occupation ratio is reduced after the product is used

The percentage of infraocular wrinkles decreased by 10.07% after 0.5 hours of application, compared to the baseline value before application (T0). The rank sum test analysis shows that the difference from the baseline value T0 is significant, and the ratio of the area of the wrinkles under the eyes is reduced after the product is used.

(VII) analysis of the volume of the eye pouch

The results are shown in table 13, table 14 and fig. 7:

TABLE 13 pouch volume descriptive statistics (mm) ³ ,n＝32)

Point in time	Number of people	Mean value of	Standard deviation of	Standard error of	Minimum value	Maximum value	Median number
								T0	32	237.28	75.16	13.29	114.13	395.29	216.83
T0.5h	32	227.52	80.62	14.25	106.59	377.45	207.57a

TABLE 14 differential analysis of pouch volume (mm) ³ ,

n＝32)

Point in time	Mean ± standard deviation	P value (vs. T0)
			T0	237.28±75.16	/
T0.5h	227.52±80.62	<0.01

As can be seen from tables 13 and 14 and fig. 7:

the pouch volume value exhibited a downward trend after use of the product compared to the baseline value before use (T0). Compared with the baseline value before use, the pouch volume value is reduced by 4.12% after the product is used for 0.5 hour, and the pouch volume is reduced after the product is used for 0.5 hour, and the pouch volume is significantly different from the baseline value T0 through paired T test analysis.

(VIII) analysis of Water content of stratum corneum of skin under eye

The results are shown in FIG. 8:

as can be seen from fig. 8: the water content of the stratum corneum below the eye shows an increasing trend after use of the product compared to the baseline value before use. The water content of the stratum corneum of the skin under the eyes rose by 6.26% after 0.5 hours of use of the product. The difference between T0.5 and the baseline value T0 is significant by the analysis of the paired T test, and the water content of the skin stratum corneum under the eyes is increased after 0.5 hour of using the product.

5. Instant experimental conclusion

There is a tendency that the under-eye skin glossiness is improved after the use of the eye mask products of examples 1 to 3; the skin under the eyes tends to turn white; the canthus and infraocular wrinkles have a tendency to improve; a reduction in pouch volume; there is a tendency for the water content of the stratum corneum of the skin under the eye to increase.

6. Long-acting effect: test results for D7 and D28 days

(one) under-eye skin gloss analysis

The results are shown in table 15, table 16 and fig. 9:

TABLE 15 under-eye skin gloss values descriptive statistics (n ═ 30)

Point in time	Number of people	Mean value of	Standard deviation of	Standard error of	Minimum value of	Maximum value	Median value
								D0	30	3.61	1.91	0.35	0.96	7.52	3.06
D7	30	4.42	1.82	0.33	0.91	8.23	4.04
								D28	30	6.60	1.75	0.32	3.31	10.57	6.58

TABLE 16 skin gloss value under eyesAnalysis of anisotropy: (

n＝30)

Point in time	Mean ± standard deviation	P value (vs. T0)
			D0	3.61±1.91	/
D7	4.42±1.82	<0.01
			D28	6.60±1.75	<0.01

As can be seen from tables 15 and 16 and fig. 9, the under-eye skin gloss values exhibited a rising trend after the product was used, compared to the baseline value before use (D0). The under-eye skin gloss values increased by 22.44% and 82.83% after 7 days (D7) and 28 days (D28) of use of the product, and were significantly different from the baseline values as analyzed by the t-test, and the under-eye skin gloss values were higher than the baseline values after use.

(second) under-eye skin color ITA-degree analysis

The results are shown in tables 17 and 18 and fig. 10:

table 17 under-eye skin gloss values descriptive statistics (n ═ 30)

Point in time	Number of people	Mean value of	Standard deviation of	Standard error of	Minimum value	Maximum value	Median value
								D0	30	19.06	6.84	1.25	6.70	36.46	18.84
D7	30	23.74	4.85	0.89	15.10	36.29	23.28
								D28	30	27.23	6.01	1.10	15.94	37.06	26.94

TABLE 18 differential analysis of under-eye skin gloss values (

n＝30)

Point in time	Mean ± standard deviation	P value (vs. T0)
			D0	19.06±6.84	/
D7	23.74±4.85	<0.01
			D28	27.23±6.01	<0.01

As can be seen from table 17, table 18, and fig. 10, the under-eye skin color ITA ° value exhibited a gradual upward trend after use of the product, increased by 24.55% and 42.86% after use of the product for 7 days (D7) and 28 days (D28), and was significant in difference from the baseline value T0 by T-test analysis, as compared to the pre-use baseline value (D0), and the under-eye skin color ITA ° value was higher than the baseline value after use of the product for 7 days (D7) and 28 days (D28).

Analysis of Ocular skin Red pigment (EI) value and Melanin (MI) value

The results are shown in tables 19 and 20 and FIG. 11:

table 19 ocular skin red pigment values and melanin values descriptive statistics (n ═ 30)

TABLE 20 analysis of the difference between the values of skin red pigment and melanin under the eyes: (

n＝30)

As can be seen from tables 19, 20 and fig. 11, the under-eye skin red pigment values changed slightly after 7 days of product use (D7) compared to the baseline value before use (D0), but did not differ statistically from baseline value D0 as analyzed by the t-test; the product had a tendency to decline after 28 days of use (D28), which was 12.12% less than D0. The difference of the product and a baseline value T0 is significant through matching T test analysis, and the value of the skin red pigment under the eyes is lower than the baseline value after the product is used for 28 days.

The sub-ocular skin red and melanin values tended to decrease after 7 days (D7) and 28 days (D28) of use of the product by 3.47% and 5.18%, respectively, compared to the pre-application baseline value (D0). The differences from the baseline value T0 were not statistically significant as analyzed by the paired T-test.

(IV) analysis of canthus and infraocular wrinkle volumes

The results are shown in tables 21 and 22 and fig. 12:

TABLE 21 Ocular Angle and infraocular wrinkle volume descriptive statistics (mm) ³ ,n＝30)

TABLE 22 analysis of volume difference (mm) between canthus and infraocular wrinkles ³ ,

n＝30)

As can be seen from tables 21 and 22 and fig. 12: both the canthus and sub-ocular wrinkle volume values exhibited a decreasing trend after use of the product compared to the pre-use baseline value (D0).

Among them, the volume value of canthus wrinkles was decreased by 2.89% after 7 days (D7) of using the product, compared with the baseline value before use (D0). The difference from the baseline value T0 has no statistical significance through the analysis of the paired T test; after 28 days of use (D28), there was a 9.03% drop, a significant difference from baseline value D0 as analyzed by paired t-tests, and a reduction in canthus wrinkle volume after 28 days of use of the product.

The sub-ocular wrinkle volume value decreased by 4.91% after 7 days of product use (D7) compared to the pre-use baseline value (D0). Through the rank sum test analysis, the difference from the baseline value D0 has statistical significance, and the volume of the wrinkles under the eyes is reduced after 7 days of using the product; after 28 days of use of the product (D28), the decrease was 2.75%. The difference from the baseline value D0 was not statistically significant by rank-sum test analysis.

(V) analysis of canthus and infraocular wrinkle area

The results are shown in table 23, table 24 and fig. 13:

descriptive statistics of canthus and infraocular wrinkle area of table 23 (mm) ² ,n＝30)

Differential analysis of area of canthus and infraocular wrinkles (mm) in table 24 ² ,

n＝30)

As can be seen from tables 23 and 24 and fig. 13: both the canthus and infraocular wrinkle area values exhibited a downward trend after use of the product compared to the pre-use baseline value (D0).

Among them, the area value of canthus wrinkles was reduced by 8.53% and 10.50% after 7 days and 28 days using the product, respectively, compared with the baseline value before use (D0). The difference of the ratio t test and analysis with the base line value D0 is significant, and the wrinkle area of the canthus after the product is used is reduced.

The sub-ocular wrinkle area values were reduced by 7.42% and 6.48% after 7 and 28 days of product use, respectively, compared to the pre-use baseline value (D0). The paired T-test analysis shows that the difference from the baseline value T0 is significant, and the wrinkle area under the eyes is reduced after the use.

(VI) analysis of area ratio of canthus and infraocular wrinkles

The results are shown in table 23, table 24 and fig. 14:

table 25 canthus and infraocular wrinkle area percentage descriptive statistics (%, n ═ 30)

Differential analysis of area of canthus and infraocular wrinkles (mm) in table 26 ² ,

n＝30)

As can be seen from tables 25 and 26 and fig. 14: the canthus and infraocular wrinkle area ratios exhibited a downward trend after use of the product compared to the pre-use baseline value (D0) compared to the pre-use baseline value (D0)

Wherein the percentage of cornear wrinkle area decreased by 6.82% and 9.15% after 7 days and 28 days, respectively, of the product compared to the baseline value before use (D0). The difference of the product and a base line value D0 is significant through paired t test analysis, and the area ratio of canthus wrinkles is reduced after the product is used

The under-eye wrinkle area percentage decreased by 6.70% and 5.79% after 7 days and 28 days, respectively, of the product, compared to the baseline value before use (D0). The analysis of the paired t test shows that the difference from the baseline value D0 is significant, and the area ratio of the wrinkles under the eyes is reduced after the product is used.

(VII) analysis of the volume of the eye pouch

The results are shown in table 27, table 28 and fig. 15:

TABLE 27 pouch volume descriptive statistics (mm) ³ ,n＝30)

Group of	Number of people	Mean value of	Standard deviation of	Standard error of	Minimum value	Maximum value	Median value
								D0	30	133.88	45.13	8.24	55.37	211.05	136.27
D7	30	129.93	45.47	8.30	48.71	203.56	138.12
								D28	30	126.94	43.96	8.03	51.73	199.62	125.10

TABLE 28 differential analysis of pouch volume (mm) ³ ,

n＝30)

Group of	Mean ± standard deviation	P value (vs. D0)
			D0	133.88±45.13	/
D7	129.93±45.47	ns
			D28	126.94±43.96	<0.05

The results are shown in table 27, table 28 and fig. 15: the pouch volume value exhibited a downward trend after use of the product compared to the pre-use baseline value (D0). Compared with the baseline value before use, the pouch volume value is reduced by 2.95 percent after the product is used for 7 days, and has no statistical significance with the baseline value D0 through the analysis of paired t test; after 28 days of product use, a 5.18% drop was observed, which was statistically significant to baseline value D0, and a reduction in pouch volume after 28 days of product use.

(VIII) analysis of Water content of stratum corneum of skin under eye

The results are shown in tables 29 and 30 and fig. 16:

table 29 moisture content of stratum corneum under eye descriptive statistics (c.u., n ═ 30)

Surface 30 sub-ocular stratum corneum moisture assay (c.u.,

n＝30)

group of	Mean ± standard deviation	P value (vs. D0)
			D0	74.38±9.34	/
D7	76.50±7.20	ns
			D28	78.13±6.62	ns

As can be seen from table 29, table 30 and fig. 16, the moisture content of the stratum corneum under the eye showed an increasing trend after the product was used, compared to the baseline value before use. The water content of the stratum corneum of the skin under the eyes increased by 2.85% and 5.04% after 7 days and 28 days, respectively, of the product. The paired t test and rank sum test analysis did not have statistical significance to the baseline value D0.

7. Conclusion of long-term test

8. Sensitive muscle test

The test was carried out according to technical Specification for cosmetic safety 2015.

The test results are given in the following table:

as can be seen from the above table, after using the eye masks of examples 1 to 3 of the present application for 4 weeks, the skin reaction scores of 33 subjects were all 0, and no adverse reaction or adverse event occurred. Thus, the eye mask formulation is mild and not easy to be allergic.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A formula of a multi-effect eye mask with instant and long-acting effects is characterized by comprising a first component, a second component and water;

the first component includes:

EX-distillers meal extract MR, schizophyllan-G1, nicotinamide, erythritol;

the second component includes:

2. The formulation of a multi-effect ophthalmic membrane with both immediate and long-lasting effects as claimed in claim 1,

the first component further comprises:

the second component further comprises:

3. The formulation of a multi-effect ophthalmic membrane with both immediate and long-lasting effects as claimed in claim 2,

the eye mask comprises the following components in percentage by mass:

0.1% -0.5% EX-lees extract MR, 0.1% -0.5% schizophyllan-G1, 1% -5% nicotinamide, 2% -5% erythritol, 0.1% -2.5% Haloxyyl PH MBAL, 0.1% -0.5% BioGenic CellLite-220, 0.5% -2% MOISTSELD HA, 0.1% -2% of whole human source ^TM Collagen, 0.1% -0.5% SENS' FLOWER SD-SC, 0.5% -2% NaturalSuperM @ 2-alpha GG-40, 1% -5% XEP-018 conopeptide, 1% -5% glyceryl polyether-26, 1% -5% glycerin, 0.1% -0.3% low molecular sodium hyaluronate, 0.2% -0.4% xanthan gum, 2% -5% 1.3-butanediol, 0.3% -0.6% 1, 2-hexanediol, 0.3% -0.6% p-hydroxyacetophenone, 0.03% -0.05% hydrogenated castor oil, 0.001% -0.003% bergamot fruit oil, 0.001%-0.003% ondansetron oil;

the balance being water.

4. The formulation of a multifunctional eye mask with both immediate and long-lasting effects according to any one of claims 1 to 3,

the multifunctional eye mask with instant and long-acting effects is colorless transparent liquid.

5. The formulation of a multi-effect ophthalmic membrane with both immediate and long-lasting effects as claimed in claim 4,

the EX-distillers meal extract MR comprises the following components: rice fermentation product filtrate, butanediol, water, citric acid, sodium citrate, 1, 2-hexanediol and p-hydroxyacetophenone.

6. The formulation of a multi-effect ophthalmic membrane with both immediate and long-lasting effects as claimed in claim 4,

the NaturalSuperM @ 2-alpha GG-40 comprises the following components: glycerol glucoside, water, 1, 2-pentanediol.

7. The method for preparing a multifunctional eye mask with both immediate effect and long effect according to any one of claims 1 to 6,

8. The method for preparing a multifunctional eye mask with both immediate effect and long-acting effect as claimed in claim 7,

the step of adding water and the first component into the water phase pot, and heating and preserving heat is to heat the water and the first component to 80-85 ℃ for heat preservation.

9. The method for preparing a multifunctional eye mask with both immediate effect and long-acting effect as claimed in claim 7,

and in the step of adding the first material into an emulsifying pot, homogenizing and cooling, the temperature is reduced to 44-46 ℃.

10. The method for preparing a multifunctional eye mask with both immediate effect and long-acting effect as claimed in claim 7,

and in the step of adding the first material into an emulsifying pot, homogenizing and cooling, the homogenizing speed is 1000 r/min-2000 r/min.