KR102613845B1 - Cosmetic composition for improvement of skin derived from natural products - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement derived from natural products, which not only has an excellent skin regenerative effect and an excellent effect of delivering oil and moisture into the skin, but also has an excellent effect on the skin of the active ingredients contained in this cosmetic composition. It has the effect of promoting internal absorption, and has a unique effect that can increase the effect of the active ingredients of the cosmetic composition. In addition, it has the effect of promoting the absorption of polydioxanone into the skin, and has a unique effect of promoting the skin regeneration ability of polydioxanone.

Description

Cosmetic composition for improvement of skin derived from natural products}

The present invention relates to a cosmetic composition derived from natural products for improving skin.

The skin is a membrane that covers the outside of the body and is the primary defense layer of the human body. It performs various physiological functions to protect the body from environmental factors such as various external stimuli, obstacles, and dryness, thereby protecting internal organs and other internal organs. It plays an important role in maintaining biological homeostasis by protecting and regulating the body. Like other organs in the human body, the important skin also gradually ages as we age, and the secretion of various hormones that regulate metabolism decreases, the function of immune cells and the activity of cells decrease, and as a result, the skin Phenomenon such as loss of elasticity, keratinization, wrinkle formation, and skin atrophy occur. This skin aging phenomenon not only causes wrinkles on the skin surface but also changes the overall line of the face. In addition, externally, the content of ultraviolet rays increases due to the destruction of the ozone layer, and as environmental pollution becomes more severe, excessive physical and chemical stimulation and stress caused by the increase in free radicals and active harmful oxygen, etc., affect the normal function of the skin. It can damage the skin by promoting the formation of spots, freckles, etc. due to melanin deposition and skin aging.

In order to prevent skin aging and damage caused by these factors and maintain stronger and more beautiful skin, bioactive substances obtained from various animals, plants, and microorganisms are added to cosmetics to maintain the skin's inherent functions and activate skin cells. Efforts to maintain skin health by promoting skin metabolism are continuing, and cosmetics and external skin preparations are being developed accordingly. In order to provide nutrition to damaged skin, apply natural ingredients such as cucumbers or tangerines, or pack agents such as massage cream, nutritional pack, cleansing pack, or bleaching pack, and then cover the face with a beauty tissue or other auxiliary means. Generally used.

However, the amount of these substances is limited due to safety issues such as irritation, erythema, and redness when applied to the skin, or their effects are so minimal that they cannot be expected to substantially improve skin function or heal wounds. Therefore, there is an urgent need for the development of new external skin compositions that are safer and more effective than existing skin compositions. Therefore, there is a need for the development of cosmetic compositions, beauty sheets, and patches that effectively absorb functional substances into the skin, have excellent skin function improvement effects, and are less irritating.

Recently, many products using natural products have been developed in the cosmetics industry to reduce skin irritation caused by chemicals. Not only do natural ingredients have fewer side effects on the skin, but their development value as cosmetic raw materials is increasing as consumers' response to cosmetics using natural ingredients has recently increased. Accordingly, the demand for cosmetics using natural ingredients that have fewer side effects and are highly responsive to consumers is gradually increasing. Referring to Korean Patent No. 10-2265128, a cosmetic composition containing spicule, a natural material, is used for skin treatment. In addition to research on materials with excellent biosafety and skin improvement effects, various studies are being actively conducted on the development of multi-functional cosmetics that can effectively improve skin health by applying them in combination.

Korean Patent No. 10-2265128 (publication date 2021.06.14)

The present invention was invented to solve the problems of the prior art and has the following purposes.

According to one embodiment of the present invention, 20 parts by weight of spicule, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and 5 parts by weight of Carum Petroselinum Extract The purpose is to provide a cosmetic composition comprising a cosmetic composition and a cosmetic patch for topical skin care.

This is explained in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in the present invention fall within the scope of the present invention. Additionally, the scope of the present invention cannot be considered limited by the specific description described below.

One embodiment of the present invention for achieving the above object is 20 parts by weight of spicules, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and Carum Petroselinum extract. Extract) provides a cosmetic composition containing 5 parts by weight.

In addition, one embodiment of the present invention includes 20 parts by weight of spicules, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and 5 parts by weight of Carum Petroselinum Extract. It provides a cosmetic composition comprising: a particle size of polydioxanone of 10 μm to 30 μm.

In addition, one embodiment of the present invention includes 20 parts by weight of spicules, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and 5 parts by weight of Carum Petroselinum Extract. Contains retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, ceramide, collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, alpha-bisabolol, tocopherol, It contains at least one selected from the group consisting of tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, vitamins A, D, E, and natural extracts, and the natural extracts include aloe, green tea, ginseng, red ginseng, pearl, and wood vinegar. , pine needles, ginkgo leaves, propolis, mulberry leaves, silkworm, snail slime, kakadu plum, camu camu, yasa palm, squalane, caviar, broccoli, blueberry, witch hazel, acerola, chlorella, mangosteen, guava, cornelian cherry, carrot. , caffeine, hamamelis, spirulina, salmon roe, gamtae, giant kelp, dried seaweed, portulaca quince, green seaweed, agar agar, mulberry, raspberry, raspberry, hijiki, edelweiss, chamomile, lavender, peppermint, eucalyptus, lemon balm, oregano, Provided is a cosmetic composition extracted from one or more selected from the group consisting of tea tree, goldenrod, Houttuynia cordata, sea buckthorn, and citron.

In addition, one embodiment of the present invention includes 20 parts by weight of spicules, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and 5 parts by weight of Carum Petroselinum Extract. It provides a cosmetic patch for topical skin care, comprising a cosmetic composition wherein the polydioxanone has a particle size of 30 to 60 μm.

In addition, one embodiment of the present invention includes 20 parts by weight of spicules, 10 parts by weight of polydioxanone, 2 parts by weight of PHA (poly hydroxy acid), and 5 parts by weight of Carum Petroselinum Extract. A step of attaching a cosmetic patch for topical skin care to the skin, comprising a cosmetic composition wherein the polydioxanone has a particle size of 30 to 60 μm, and applying a cosmetic patch of 3 MHz to 7 MHz to the cosmetic patch for topical skin care. A method of promoting skin absorption of the cosmetic composition is provided by applying ultrasound at an output of 1W/cm ² .

The cosmetic composition according to one embodiment of the present invention has excellent skin regeneration effect on its own. In addition, the cosmetic composition of the present invention not only has an excellent effect of delivering oil and moisture into the skin, but also has the effect of promoting absorption into the skin of the active ingredients contained in the cosmetic composition. There is a unique effect that can increase the effect shown. In addition, it has the effect of promoting the absorption of polydioxanone into the skin, and has a unique effect of promoting the skin regeneration ability of polydioxanone.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples. The terms used below are only used to describe specific examples. Therefore, for example, a singular expression includes a plural expression, unless the context clearly requires it to be singular. In addition, terms such as "include" or "provided" used in the present application are used to clearly indicate the presence of feature steps, functions, components, or combinations thereof described in the specification, and are used to clearly indicate the presence of other features or elements. It should be noted that it is not used to preliminarily rule out the existence of any step, function, component, or combination thereof.

Meanwhile, unless otherwise defined, all terms used in this specification should be viewed as having the same meaning as generally understood by those skilled in the art to which the present invention pertains. Therefore, unless clearly defined in this specification, specific terms should not be interpreted in an overly idealistic or formal sense. The terminology used herein is for describing embodiments and is not intended to limit the invention. As used herein, singular forms also include plural forms, unless specifically stated otherwise in the context. The term "bond" used below may include both physical and chemical attachment, connection, etc., and may mean a state in which substances do not separate from each other due to a specific bonding force between substances, but is not limited thereto.

In this specification, "sponge" is also called a sponge. It is a primitive marine organism without muscles, nerves, or organs, and there are more than 5,000 species and are found regardless of the depth of the sea. “Bone fragments” are needle-shaped tissues that exist in the body of invertebrates and are responsible for the function of the skeleton, and their main components are silicic acid and calcium carbonate. The fibrous skeleton of sponges has the ability to absorb through capillary action and is used in various fields, including medical applications. As an example of use, bone fragments from sponges contain antibiotics for the treatment of osteomyelitis and are used for the treatment and prevention of the disease. The sponge may be a sponge belonging to the species Spongilla lacustris L. , Spongilla fragilis Leidy , and Ephydatia fluviatilis .

In this specification, “spicule” is a bone fragment of a sponge, and has a fine needle-like structure that penetrates the skin layer to form a micro passage, so the penetration effect of the applied cosmetic ingredient can be significantly increased. The microneedles can promote skin regeneration of collagen, which acts on artificial wound healing, and increase bioactivity by increasing skin tissue temperature. The spicule is a substance that is harmless to the human body, and when it comes into contact with the skin, it irritates the skin, forms fine pores, and can be dissolved or discharged from the body.

In this specification, “polydioxanone” is a substance that is friendly to water and is easily hydrolyzed, causing fewer side effects on the human body. It is a polymer material in which the ether-ester unit is repeated several times. The polydioxanone can promote the remodeling ability of skin collagen as it is hydrolyzed by moisture in the body. This means that the water-loving polydioxanone surface is a good surface for macrophages to adhere to, and thus makes it easy to produce collagen by reaction to external factors.

In this specification, “Carum petrolcellinum” may refer to a biennial plant of the dicotyledonous Umbrella Apiaceae family native to the Mediterranean coast and southern France. The Carum petrolcellinum is often used as a spice in basic Western cuisine, and is highly nutritious as it contains a large amount of vitamins and calcium.

In this specification, “active ingredient” refers to a cosmetic or nutritional ingredient that can exhibit an effective effect on the skin.

As used herein, “skin regeneration” refers to the recovery process of skin tissue against damage caused by external and internal causes. Damage due to external causes may include ultraviolet rays, external contaminants, wounds, trauma, etc., and damage due to internal causes may include stress.

As used herein, “skin microrelief” refers to a three-dimensional microstructure formed by fine lines on the skin surface. Separate from elasticity, wrinkles, and moisturization, it is an indicator of skin condition. More specifically, skin texture is a reliable indicator of aging that can detect aging before it begins. In addition, skin texture reflects the inner state of the skin rather than simply the rough and smooth state of the skin surface. The skin texture on the surface of the skin is only an externally exposed part of the overall skin structure, and damaged skin texture means changes in the skin. It is reflected.

In this specification, “skin texture improvement” refers to the process of recovering skin texture when it is damaged as described above.

In this specification, “skin elasticity” is expressed by elastic fibers composed of elastin present in the dermis layer, and these elastic fibers have a very low elastic modulus like rubber, so they are easily deformed even by small forces, and When the force is removed, it easily returns to its original state. In addition, traction fibers take the form of microfibrils embedded in an amorphous matrix called elastin, and elastin is a very unique fiber found only in elastic fibers called desmosine and isodesmosine, which are derived from lysine. It is a protein composed of amino acids. These desmosins and isodesmosins form cross-links within long peptide chains, and this structure gives elastin rubber-like properties.

In this specification, “enhancing skin elasticity” refers to the existence of elastic fibers made of elastin together with collagen fibers called collagen, and skin elasticity is maintained or increased in the presence of sufficient elastin and collagen.

In this specification, “skin wrinkles” refers to fine lines that occur as the skin ages, and can be caused by genes, a decrease in collagen and elastin present in the skin dermis, and external environments.

In this specification, “skin wrinkle improvement” refers to suppressing or inhibiting the formation of wrinkles on the skin or alleviating wrinkles that have already been formed.

In this specification, “oil and moisture” refers to oil and/or moisture required by the skin, and can enable skin tissue to be maintained by delivering oil and moisture to the skin.

In this specification, “soluble film” refers to a film that dissolves and penetrates into the skin when applied or attached to the skin, leaving little residue and requiring no separate washing or cleansing to remove or peel off. The soluble film can be melted and then applied, or it can be directly attached to the area requiring care and then allowed to dissolve.

In this specification, “hydrogel” refers to a gel that uses water as a dispersion medium. The hydrosol loses its fluidity due to cooling, or the hydrophilic polymer with a three-dimensional network structure and microcrystalline structure expands by containing water. It can be formed by: Hydrogels made of electrolyte polymers often exhibit high absorbency and are used in many fields as absorbent polymers. Among hydrogels, there are some whose expansion ratio changes discontinuously due to phase transition due to temperature, pH, etc.

As used herein, “temperature-sensitive hydrogel” refers to a hydrogel in which phase transition occurs from sol to gel or gel to sol depending on temperature, and the phenomenon of sol changing to gel is called gelation. ), and gelation in this specification refers to the state in which the polymer has viscoelasticity and forms a three-dimensional network structure as the temperature increases, remaining insoluble in the solvent. In particular, it exists in a sol state at room temperature and can gel when applied to the skin surface and the temperature rises.

In this specification, “local skin” refers to a part of tissue such as the eye area, mouth area, nasolabial folds, cheekbones, cheeks, chin, hands, feet, elbows, chest, etc., or the skin of a specific part of an individual.

In this specification, “skin care” refers to actions for the effects of skin regeneration, skin texture improvement, skin elasticity enhancement, or skin wrinkle improvement, but is not limited thereto, as long as it is for the purpose of improving skin tissue or preventing aging, etc. may be included.

As used herein, “skin irritation” refers to reversible inflammatory changes in the skin caused by exposure to a cosmetic composition.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

The present invention is a cosmetic composition containing spicules and polydioxanone particles. In contrast, the present invention is a cosmetic composition containing a skin absorption stimulation accelerator. Specifically, according to one embodiment of the present invention, the cosmetic composition may further include cosmetic active ingredients.

The cosmetic composition may be formed in the form of a soluble film. The soluble film cosmetic composition may be used to regenerate skin, improve skin texture, or promote skin absorption of the cosmetic composition.

Specifically, the spicule may be 0.001 to 20 parts by weight when the total cosmetic composition is 100 parts by weight. The spicule has the advantage of showing excellent skin regeneration, wrinkle improvement, or skin texture improvement effects within the above range, and has the advantage of stabilizing the formulation of the composition.

Additionally, the particle size of the spicule may be 40 μm to 200 μm, more specifically 60 μm to 100 μm, and most specifically 70 μm to 90 μm.

In this way, the spicule not only helps cell regeneration by penetrating into the basal layer of the skin and activating the therapeutic immune system of the human body, but also can reach the dermis layer when it penetrates into the pores, thereby injecting the polydioxanone into the skin. It plays a role in helping it be conveyed deeply. Additionally, it can promote absorption of cosmetic active ingredients into the skin.

Specifically, the polydioxanone particles may be 5 to 10 parts by weight when the total cosmetic composition is 100 parts by weight. The polydioxanone particles can effectively promote absorption of the skin layer by the spicule within the above range, and for the range beyond this, the skin layer is absorbed by increasing the amount of the polydioxanone particles. cannot have a significant increase in effect. Specifically, assuming that the cosmetic composition is 100 parts by weight and the spicule is 20 parts by weight, when the polydioxanone particles include 10 parts by weight or more, the polydioxanone particles A significant increase in effect on the skin cannot be expected as the dosage increases. This is because the delivery and promotion of skin penetration of Polydioxanone is achieved by spicules, and the maximum amount of promotion of Polydioxanone is determined in conjunction with the amount of spicules.

Additionally, the particle size of the polydioxanone may be 1 μm to 100 μm. Preferably, the particle size of the polydioxanone may be 10 μm to 30 μm. If the particle size of the polydioxanone is smaller than 10 μm, there is a problem that the process cost increases excessively during the freeze-drying and grinding process. If the size of the polydioxanone particles is larger than 30 μm, it may have difficulty moving to the stratum corneum, epidermis, and dermis layers of the skin, making it difficult to promote the remodeling ability of skin collagen.

Specifically, the skin absorption stimulation accelerator may be AHA (Alpha hydroxy acid), BHA (Beta hydroxy acid), and PHA (Poly hydroxy acid). Additionally, the skin absorption stimulation accelerator may be provided mixed with Carum Petroselinum Extract. According to an embodiment of the present invention, the skin absorption stimulation accelerator is PHA (poly hydroxy acid) and Carum Petroselinum Extract (Table 1). Through the skin absorption stimulation accelerator, skin penetration of the polydioxanone can be promoted without excessive skin stimulation.

The PHA (poly hydroxy acid) may be 0.1 to 2 parts by weight when the total cosmetic composition is 100 parts by weight. Preferably, the PHA (poly hydroxy acid) may be 1 part by weight to 2 parts by weight when the total cosmetic composition is 100 parts by weight. If the PHA (poly hydroxy acid) is not sufficiently included, such as less than 0.1 part by weight, it cannot stimulate skin absorption to a degree that sufficiently promotes skin penetration of the polydioxanone. If the PHA (poly hydroxy acid) is included in excess of 2 parts by weight, it may cause excessive skin irritation and damage the skin.

Additionally, the Carum Petroselinum Extract may be 2 to 2.5 times the weight of the PHA (poly hydroxy acid). If the Carum Petroselinum Extract is not included sufficiently, such as less than twice the weight of the PHA (poly hydroxy acid), it may not sufficiently compensate for skin irritation and may damage the skin.

Specifically, the amount of the active ingredient may be 0.001 to 30 parts by weight when the total cosmetic composition is 100 parts by weight. The amount of the active ingredient may be selected differently depending on the type of ingredient and formulation.

In addition, the active ingredients include retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, ceramide, collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, alpha-bisabolol, It may be one or more selected from the group consisting of tocopherol, tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, batiman A, vitamin D, vitamin E, and natural extracts, but is not limited thereto.

In addition, the natural extracts include aloe, green tea, ginseng, red ginseng, pearl, wood vinegar, pine needles, ginkgo leaves, propolis, mulberry leaves, silkworm, snail slime, kakadu plum, camu camu, yassa palm, squalane, caviar, broccoli, and blue. Berries, witch hazel, acerola, chlorella, mangosteen, guava, cornelian cherry, carrots, caffeine, hamadelis, spirulina, salmon roe, gamtae, giant kelp, dried seaweed, portulaca quince, green seaweed, agar, mulberry, raspberry, raspberry, hijiki, It may be extracted from one or more selected from the group consisting of moss, edelweiss, chamomile, lavender, lepermint, eucalyptus, lemon balm, oregano, tea tree, goldenrod, Houttuynia cordata, sea buckthorn and citron, but is not limited thereto.

In addition, the cosmetic active ingredient may be in the form of a solution, lotion, cream, gel, essence, skin-adhesive cosmetic, lotion, ointment, oil, suspension, emulsion, or spray, but is not limited to the above formulation.

Specifically, the soluble film includes pullulan, gelatin, agar, carrageenan, alginate, chitosan, chitosan derivative, starch, galatomannan, glucomannan, guar gum, locast bean gum, pluronic, algin, xanthan, gellan, poly One or more polymers selected from the group consisting of vinylpyrrolidone (PVP), polyvinyl alcohol (PVA), polyethylene oxide (PEO), polyvinyl acrylic acid (PVAc), and polymethacrylic acid (PMAc) may be included as a film former. , but is not limited to this. More specifically, the film former may include flurane and polyvinylpyrrolidone.

According to one embodiment of the present invention, the soluble film may be a hydrogel. The hydrogel may be a temperature-sensitive hydrogel. The temperature-sensitive hydrogel can be applied directly to the skin surface or attached in the form of a mask sheet or beauty patch, and can be fully effective by increasing the retention and attachment time of the active ingredient through temperature-dependent gelation. there is.

Specifically, the hydrogel may include a branched gelling polymer, an electrolytic gelling polymer, a skin compatibility enhancer, a natural biological material, a polyhydric alcohol, a functional additive, and water.

More specifically, the branched gelling polymer may be one or more polymers selected from the group consisting of galactomannan, glucomannan, guar gum, locast bean gum, carob bean gum, and pluronic, and the electrolytic gelling polymer may be It may be one or more polymers selected from the group consisting of agar, algin, carrageenan, xanthan, and gellan. According to one example of the present invention, a hydrogel composition containing carob bean gum and carrageenan as polymers along with spicules and polydioxaone was prepared (Table 1).

According to one embodiment of the present invention, a beauty sheet containing a cosmetic composition containing the spicule and polydioxanone can be provided. Specifically, the beauty sheet may be used to regenerate skin, promote oil and moisture absorption, or promote absorption of active ingredients. The active ingredients that can be contained and delivered in the cosmetic composition of the present invention are as described above.

Materials that can be used as a support for the sheet include rayon, urethane, latex, acrylic, etc., but are not limited to these and can be woven into fabric or knitted material or modified appropriately using materials that can minimize exposure to the outside. there is.

According to another embodiment of the present invention, it is possible to provide a cosmetic patch for topical skin care containing a cosmetic composition containing the spicule and polydioxanone. Specifically, the cosmetic patch for topical skin care can be appropriately manufactured in the size and shape required for each topical skin to which it is applied for topical skin care.

The beauty sheet or the beauty patch can be used in various ways. It is used by impregnating the skin with a liquid containing active ingredients (ingredients for moisturizing, anti-allergy, antibacterial, whitening, wrinkle improvement, etc.) and attaching it to the skin and detaching it after a certain period of time. Among these, spicule and poly A cosmetic composition containing polydioxanone can gel in response to skin temperature and penetrate into the skin.

According to another embodiment of the present invention, a) attaching a cosmetic patch for topical skin care containing a cosmetic composition to the skin; and b) applying ultrasound to the cosmetic patch for topical skin care at an output of 1 W/cm ² to apply the cosmetic patch. A method of promoting skin absorption of the composition can be provided. Descriptions of the specific cosmetic composition and cosmetic patch for topical skin are as described above. Through this, it can be used to promote skin regeneration and the delivery of oil and moisture or active ingredients.

The ultrasonic waves may be generated by a separate device. The ultrasonic device can identify the skin surface to which the topical skin cosmetic patch is attached. The ultrasound may target a region of interest (ROI). The target area may be located in subcutaneous tissue below the target local skin surface. The subcutaneous tissue may include any or all of the epidermal layer, cortical layer, fat layer, SMAS layer, and muscle layer. The frequency of the ultrasonic waves may be 1 MHz to 10 MHz. Preferably, the frequency of the ultrasonic waves may be 3 MHz to 7 MHz. The ultrasound may be irradiated to a depth of 0.001 mm to 10 mm below the outer surface of the skin. Preferably, the ultrasound waves can be irradiated to a depth of 3.5 mm to 4.0 mm below the outer surface of the skin. Through this, the absorption of polydioxanone can be promoted non-invasively from the dermis layer to the fascia layer.

Hereinafter, examples and comparative examples will be described to aid understanding of the present invention. However, the following description only corresponds to an example of the content and effects of the present invention, and the scope and effects of the present invention are not necessarily limited thereto.

Example Preparation 1.

A cosmetic composition containing spicules and polydioxanone was prepared by mixing the ingredients listed in Table 1 below. Specifically, the ingredients of Phase 1 were placed in a preparation tank and mixed and dissolved at Disper 1500-1700 rpm, Homo 2000-2500 rpm, Paddle 30-45 rpm, and a temperature of 40-45°C, and then completely mixed at 80°C.

The ingredients of Phase 2 were mixed for 15 to 20 minutes at Disper 1500-1700 rpm, Homo 2000-2500 rpm, paddle 30-45 rpm, and a temperature of 80-85°C, dispersed for 10 minutes at Disper 800-1200 rpm, and then mixed in Phase 1. was added to the mixture. Afterwards, it was thoroughly mixed and cooled to 60-65°C.

Afterwards, the ingredients of Phase 3 were mixed for 20 minutes at Disper 1500-1700 rpm, Homo 2000-2500 rpm, Paddle 30-45 rpm, and a temperature of 60-65°C, and then added to the mixture of Phases 1 and 2 and mixed at 60-65°C. Mixed thoroughly. Afterwards, the paddle was degassed at 15 to 22 rpm.

At this time, the average size of the mixed polydioxanone particles is 30 μm.

award ingredient Example 1 Example 2 Example 3 Prize 1 Purified water To 100 To 100 To 100 Disodium EDTA 0.02 0.02 0.02 Prize 2 glycerin 30 30 30 carob bean gum 3 10 10 Hydrolyzed sponge (spicule) 20 20 20 Polydioxanone One 4 7 Carrageenan 3 3 3 Prize 3 PEG-60 Hydrogenated Castor Oil 0.2 0.2 0.2 Caprylic/Capric Triglyceride 0.1 0.1 0.1 antiseptic 3 3 3 incense 0.1 0.1 0.1 Sum 100wt% 100wt% 100wt% award ingredient Example 4 Example 5 Example 6 Prize 1 Purified water To 100 To 100 To 100 Disodium EDTA 0.02 0.02 0.02 Prize 2 glycerin 30 30 30 carob bean gum 10 10 10 Hydrolyzed sponge (spicule) 20 20 20 Polydioxanone 10 13 16 Carrageenan 3 3 3 Prize 3 PEG-60 Hydrogenated Castor Oil 0.2 0.2 0.2 Caprylic/Capric Triglyceride 0.1 0.1 0.1 antiseptic 3 3 3 incense 0.1 0.1 0.1 Sum 100wt% 100wt% 100wt%

The high-temperature cosmetic composition prepared as described above was molded into a cosmetic sheet or patch in a fluid state at a temperature of 45 to 95°C and cooled to room temperature to prepare a hydrogel in the form of a gel.

Example Preparation 2.

Example Preparation The preparation method of Preparation 1 was followed as is, and with respect to the combination ratio of Example 4, PHA (Poly hydroxy acid) and Carum Petroselinum Extract were added to Phase 2 in different amounts as shown in Table 2 below. Mixed.

award ingredient Example 7 Example 8 Example 9 Prize 2 Poly hydroxy acid (PHA) 0.1 One 1.5 Carum Petroselinum Extract 0.2 2.5 3.5 award ingredient Example 10 Example 11 Example 12 Prize 2 Poly hydroxy acid (PHA) 2 2.5 3 Carum Petroselinum Extract 5 6 7.5

The high-temperature cosmetic composition prepared as described above was molded into a cosmetic sheet or patch in a fluid state at a temperature of 45 to 95°C and cooled to room temperature to prepare a hydrogel in the form of a gel.

Example Preparation 3.

In order to confirm the effect of the size of the polydioxanone particles, an example was prepared by limiting the size of the polydioxanone particles as shown in Table 2 below. In Examples a to g shown in Table 2 below, a hydrogel composition was prepared by mixing the same ingredients as Example 4 (20 parts by weight of spicules, 10 parts by weight of polydioxanone), and the specific mixing method was also the same. I made it happen.

sample Polydioxanone particle size (μm) Example a 10 Example b 20 Example c 30 Example d 40 Example e 50 Example f 60 Example g 70

The high-temperature cosmetic composition prepared as described above was molded into a cosmetic sheet or patch in a fluid state at a temperature of 45 to 95°C and cooled to room temperature to prepare a hydrogel in the form of a gel.

Comparative example preparation.

Mix the ingredients listed in Table 3 below to create a hydrogel composition that does not contain spicules, a hydrogel composition that does not contain polydioxanone, or a hydrogel that does not contain spicules and polydioxanone. The composition was prepared, and the specific mixing method was the same as that described in Example Preparation 1.

At this time, the average size of the mixed polydioxanone particles is 30 μm.

award ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Prize 1 Purified water To 100 To 100 To 100 Disodium EDTA 0.02 0.02 0.02 Prize 2 glycerin 30 30 30 carob bean gum 3 10 10 Hydrolyzed sponge (spicule) 0 20 0 Polydioxanone 0 0 10 Carrageenan 3 3 3 Prize 3 PEG-60 Hydrogenated Castor Oil 0.2 0.2 0.2 Caprylic/Capric Triglyceride 0.1 0.1 0.1 antiseptic 3 3 3 incense 0.1 0.1 0.1 Sum 100wt% 100wt% 100wt%

The high-temperature cosmetic composition prepared as described above was molded into a cosmetic sheet or patch in a fluid state at a temperature of 45 to 95°C and cooled to room temperature to prepare a hydrogel in the form of a gel.

Experimental Example 1. Confirmation of skin regeneration effect of spicule and polydioxanone

The skin regeneration effect through proliferation of skin cells was confirmed using CCK (Cell counting kit)-8 analysis. Human skin fibroblasts were cultured in DMEN (Dulbecco's Modification of Eagle's Medium) medium supplemented with 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin at 37°C and 5% CO2. After plating 1,000 human skin fibroblasts in a 96-well plate, spicules were treated with cell culture medium, and the degree of cell proliferation was checked after 3 days. When measuring, CCK-8 solution was added to the cell culture medium (100 μl/well) and reacted in an incubator for 3 hours, then the absorbance was measured at 450 nm, and the increase in cell number was investigated based on the results of the standard curve. The results are shown in Table 4 below.

sample Cell count after 3 days control group 1750 Spicules treated at 0.1μg/μl 3563 Spicules treated with 10μg/μl 4787 Polydioxanone 0.1 μg/μl treatment 2561 Polydioxanone 10μg/μl treatment 3142 Treated with spicules 10μg/μl and polydioxanone 10μg/μl 5482

As shown in Table 5, when spicules were treated and cultured for 3 days, a significant increase in cell number was observed compared to the control group that was not treated with spicules. Therefore, it was confirmed that when the composition containing the spicules of the present invention is applied or attached to the skin, it can exhibit an excellent skin regeneration effect by promoting the proliferation of skin cells. In addition, as the concentration of treated spicules increased, the effect of increasing the number of cells further increased. In addition, as shown in Table 5 above, when treated with polydioxanone and cultured for 3 days, polydioxanone A significant increase in cell number was observed compared to the control group that was not treated with polydioxanone. Therefore, it was confirmed that when the composition containing polydioxanone of the present invention is applied or attached to the skin, it can exhibit an excellent skin regeneration effect by promoting the proliferation of skin cells. In addition, as the concentration of treated polydioxanone increased, the effect of increasing the number of cells further increased.

In addition, as shown in Table 5, when spicules and polydioxanone were treated and cultured for 3 days, a significant increase in cell number was observed compared to when each was treated separately. Therefore, it was confirmed that when the composition containing the spicule and polydioxanone of the present invention is applied or attached to the skin, it can further promote the proliferation of skin cells, thereby showing a better skin regeneration effect.

Experimental Example 2. Measurement of skin transfer speed of oil and moisture

An experiment was conducted to confirm the effect of promoting skin absorption of oil and moisture of the cosmetic compositions prepared in Comparative Examples 1 to 3, Examples 1 to 6, and Examples a to Examples g.

Specifically, the cosmetic compositions of Comparative Examples 1 to 3, Examples 1 to 6, and Examples a to Examples g were applied, respectively, and after 30 minutes, a skin moisture meter (scalar moisture checker for skin MY-707S) was applied. ) was used to measure skin moisture.

sample Skin moisture (%) Comparative Example 1 45 Comparative Example 2 62 Comparative Example 3 49 Example 1 63 Example 2 65 Example 3 63 Example 4 64 Example 5 65 Example 6 63 Example a 71 Example b 70 Example c 67 Example d 65 Example e 64 Example f 63 Example g 61

As shown in Table 6, it was confirmed that the skin moisture level of Comparative Examples 2 to 3 containing spicules or polydioxanone was significantly higher than that of Comparative Example 1 which did not contain spicules. Additionally, , As shown in Table 6, it was confirmed that the skin moisture level of Examples 1 to 6 containing spicules and polydioxanone was higher compared to Comparative Example 2 containing only spicules.

In addition, as shown in Table 6, compared to Comparative Example 3 containing only polydioxanone, the skin moisture level of Examples 1 to 6 containing spicules and polydioxanone was was confirmed to be high.

In addition, as shown in Table 6 above, as can be seen from Examples a to g of the embodiments containing spicules and polydioxanone, the smaller the particle size of Polydioxanone, the more effective it is on the skin. It was confirmed that the moisture content also increased.

Experimental Example 3. Measurement of skin permeability of polydioxanone

To confirm the effect of promoting skin absorption of polydioxanone of the cosmetic composition containing the spicules and polydioxanone of Examples 1 to 12 and Examples a to Examples g prepared above. An experiment was performed for. The cosmetic compositions of Comparative Example 3, Examples 1 to 12, and Examples a to Examples g containing only polyxioxanone were applied and skin permeability was measured.

Specifically, the skin on the back of an 8-10 week old hairless mouse was removed, subcutaneous fat and tissues were removed with physiological saline, and the skin was spread well and used in the experiment. The stratum corneum was placed in the donor compartment of the hairless Pius skin Franz diffusion cells, sandwiched between the receptor compartments, and then applied to the surface of the skin in Comparative Example 3, Examples 1 to 12, and Examples. The cosmetic compositions of Examples a to g were applied. Phosphate buffered saline (pH 7.4) was added to the recipient compartment, and stirring was continued at 300 rpm while maintaining the temperature at 37°C. At this time, the area of the skin in contact with the recipient compartment was 2.0 cm ² and the capacity of the recipient compartment was 5 ml. After administering 1.0 ml of each sample to the skin surface, the samples were collected using a fraction collector. All collected samples were analyzed using intestinal flow fractionation.

The accumulated amount of polydioxanone particles permeated per unit area of skin was plotted against time, and then the flux of polydioxanone particles in equilibrium was calculated from the slope of the area showing linearity. Lag time (T _L ) was obtained from the time a straight line passes through the x-axis. Polydioxanone particle flow rate and permeability coefficient in equilibrium were calculated and analyzed using the following equation 1.

[Equation 1]

is the flow rate of polydioxanone particles in equilibrium state, is the area where transmission occurs, is the slope obtained by plotting the amount of polydioxanone passing through the skin at equilibrium against time, is the saturation concentration, and represents the transmission coefficient.

The skin permeability measured according to the above analysis method is shown in Table 7 below.

sample Delay time (hr) Polydioxanone flow amount (μg/(cm ² ·hr)) Transdermal penetration rate (%) Comparative Example 3 5.3 6.20 2.50 Example 1 3.3 65.33 10.54 Example 2 3.4 78.86 15.22 Example 3 3.1 113.23 29.17 Example 4 2.7 131.28 34.86 Example 5 2.8 131.78 34.92 Example 6 2.5 132.46 35.03 Example 7 2.8 132.10 35.00 Example 8 2.5 138.77 36.77 Example 9 2.2 141.16 37.41 Example 10 2.0 143.96 38.15 Example 11 1.9 147.94 39.20 Example 12 1.5 150.24 39.81 Example a 2.1 158.76 41.11 Example b 2.3 151.32 39.10 Example c 2.7 142.84 37.84 Example d 2.9 115.32 29.91 Example e 3.5 71.51 18.47 Example f 3.7 12.70 2.74 Example g 4.2 2.13 0.54

As shown in the results of Table 7, when the hydrogel compositions of Examples 1 to 12 and Examples a to g were applied compared to the case of applying Comparative Example 3, which did not contain spicules, It was confirmed that the delivery effect and skin penetration promotion effect of polydioxanone contained therein were increased. From this, in the case of the cosmetic composition containing the spicule and polydioxanone of the present invention, the intradermal delivery effect for the polydioxanone included is very excellent, and the skin care effect is very excellent. could be confirmed. In addition, looking at Examples 1 to 6 in which the amount of polydioxanone particles was varied, the larger the amount of polydioxanone particles, the more polydioxanone. It was confirmed that the delivery effect and skin penetration promotion effect were more excellent.

In addition, as shown in the results of Table 7, PHA (Poly hydroxy acid) and Carum Petroselinum Extract are more effective than the cosmetic composition containing only spicules and polydioxanone (Example 4). It was confirmed that the delivery effect and skin penetration promotion effect of polydioxanone in Examples 7 to 12 further comprising was more excellent. In addition, looking at Examples 7 to 12, it was confirmed that the greater the amount of PHA (poly hydroxy acid), the better the delivery effect and skin penetration promotion effect of polydioxanone.

In addition, looking at Examples a to g of different sizes of Polydioxanone particles, as the size of Polydioxanone particles increases, the delivery effect of Polydioxanone and the skin It was confirmed that the permeation promotion effect was lowered. It can be seen that the size of the material that can permeate through the skin is limited. However, when spicules are used in parallel, it can have some degree of permeability (when comparing Comparative Example 3 and Example g), but there is a limit to the permeability. It was confirmed that there is. Unlike Examples a to c, it can be seen that the delivery effect and skin penetration promotion effect of polydioxanone in Examples d to g are limited, and the polydioxanone particles It was confirmed that it is effective to use a size of 30 μm or less.

Experimental Example 4. Measurement of skin irritation according to the amount of PHA (poly hydroxy acid)

Regarding the dorsal skin of the hairless mouse in Experimental Example 3, the sample was exposed to the skin for 4 hours. At this time, the sample was applied to the skin, covered with a patch, and fixed with non-irritating tape. At this time, the patch was removed after 4 hours, and skin irritation (erythema and crust formation/edema formation) was checked.

At this time, the clinical symptoms and skin reaction evaluation table was judged and graded based on the criteria shown in Table 8 below.

Erythema and crust formation Edema formation reaction Rating reaction Rating No erythema at all 0 No swelling at all 0 Very mild erythema (almost noticeable to the naked eye) One Very mild swelling (almost noticeable to the naked eye) One Marked erythema 2 Mild edema (clear swelling so that the margins can be distinguished) 2 slightly severe erythema 3 Severe edema (if the swelling is about 1mm) 3 Severe erythema and crust formation 4 Severe edema (swelling of more than 1 mm and extending beyond the exposed area) 4

sample Erythema and crusting (grade) Edema formation (grade) Example 7 0 0 Example 8 0 0 Example 9 0 0 Example 10 0 0 Example 11 One 0 Example 12 One One Example 4 (comparative sample) 0 0

As shown in Table 9, it can be confirmed that there was no skin irritation in the cosmetic composition (Example 4) that did not mix PHA (poly hydroxy acid) and Carum Petroselinum Extract. In addition, even if PHA (Poly hydroxy acid) and Carum Petroselinum Extract are mixed, even in the case of cosmetic compositions (Examples 7 to 10) in which the weight of PHA (Poly hydroxy acid) is less than 2wt% You can confirm that there is no skin irritation.

Through Tables 7 and 9, the amount of PHA (poly hydroxy acid) to improve skin permeability of polydioxanone without irritating the skin is 2 parts by weight (relative to 100 parts by weight of the total cosmetic composition). It was confirmed that it was included below the value).

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. In this regard, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of the present invention should be construed as including the meaning and scope of the patent claims described below rather than the detailed description above, and all changes or modified forms derived from the equivalent concept thereof are included in the scope of the present invention.

Claims (1)

For 100 parts by weight of the total cosmetic composition,
0.001 to 20 parts by weight of cosmetic active ingredient;
Disodium EDTA 0.02 parts by weight;
30 parts by weight of glycerin;
10 parts by weight of carob bean gum;
20 parts by weight of spicules;
10 parts by weight of polydioxanone particles;
3 parts by weight of carrageenan;
1 part by weight of poly hydroxy acid (PHA);
2.5 parts by weight of Carum Petroselinum Extract;
0.2 parts by weight of PEG-60 hydrogenated castor oil;
0.1 part by weight of caprylic/capric triglyceride;
Contains 3 parts by weight of preservative and the remaining amount of purified water,
The particle size of the polydioxanone is 30 μm,
The cosmetic active ingredients include retinol, retinyl palmitate, retinyl acetate, retinoic acid, coenzyme Q10, elastin, collagen, hyaluronic acid, ceramide, collagen, caffeine, chitosan, ascorbic acid, ascorbyl glucoside, alpha-bisabolol, and tocopherol. , tocopherol acetate, arbutin, niacinamide, adenosine, retinol acetate, vitamins A, D, E, and natural extracts,
The natural extracts include aloe, green tea, ginseng, red ginseng, pearl, wood vinegar, pine needles, ginkgo biloba, propolis, mulberry leaf, silkworm, snail slime, kakadu plum, camu camu, yasa palm, squalane, caviar, broccoli, blueberry, Witch hazel, acerola, chlorella, mangosteen, guava, cornelian cherry, carrot, caffeine, hamamelis, spirulina, salmon roe, gamtae, giant kelp, balsam, portulaca quince, green seaweed, agar agar, mulberry, raspberry, raspberry, hijiki, radish, It is extracted from one or more selected from the group consisting of edelweiss, chamomile, lavender, peppermint, eucalyptus, lemon balm, oregano, tea tree, goldenrod, Houttuynia cordata, sea buckthorn and citron,
A cosmetic composition that has the effect of promoting absorption of the active ingredient into the skin, wherein the particle size of the spicule is 70 μm to 90 μm.