CN111632018B

CN111632018B - Composition containing hydrolyzed algae extract and application thereof

Info

Publication number: CN111632018B
Application number: CN202010532281.0A
Authority: CN
Inventors: 马志冰; 李信均; 潘筱叶
Original assignee: Guangdong Bawei Biotechnology Co ltd
Current assignee: Guangdong Bawei Biotechnology Co ltd
Priority date: 2020-06-12
Filing date: 2020-06-12
Publication date: 2022-05-27
Anticipated expiration: 2040-06-12
Also published as: CN111632018A

Abstract

The invention discloses a composition containing an algae hydrolysate extract and application thereof, aiming at providing a composition capable of effectively resisting photoaging, and the composition is applied to cosmetics, so that cells can be protected from being damaged by ultraviolet rays, the phenomena of rough skin, reddening, pigmentation, wrinkle deepening and thickening and the like caused by photoaging are effectively improved, and the skin is more uniform, fine and glossy; the composition comprises the following components in parts by weight: 4-12 parts of hydrolyzed algae extract, 3-9 parts of cassia wingnut leaf extract, 6-18 parts of active oxygen scavenging factor, 15-30 parts of polyalcohol, 15-25 parts of seawater and 20-40 parts of water, wherein the active oxygen scavenging factor comprises litchi peel extract and Aralia elata leaf extract; belongs to the technical field of cosmetics.

Description

Composition containing hydrolyzed algae extract and application thereof

Technical Field

The invention discloses a composition and application thereof, in particular to a composition containing an algae hydrolysis extract, and belongs to the technical field of cosmetics.

Background

Skin aging is classified into extrinsic aging and intrinsic aging. Endogenous aging, also known as natural aging, is a programmed aging process controlled by genetic factors, develops over time and has irreversibility; extrinsic aging is caused by environmental factors, the most prominent of which is skin aging caused by excessive Ultraviolet (UV) radiation, i.e., photoaging.

The human skin is mainly divided into an epidermis layer and a dermis layer, the damage lesion of the epidermis layer and the dermis layer of the skin is mainly caused by UVB, the wavelength of the UVB is mostly absorbed by protein and DNA in keratinocytes, DNA damage is directly caused, the keratinocytes are subjected to atypical hyperplasia, epitomegaly disappears, elastic fibers are arranged disorderly, and inflammatory cells are infiltrated; UVA has stronger penetrating power than UVB, easily penetrates the basal layer of human skin to cause damage of epidermal basal cells, and influences the whole dermis. The ultraviolet radiation can induce to generate a large amount of Reactive Oxygen Species (ROS) including superoxide anions, hydrogen peroxide, hydroxyl free radicals and the like, the ROS can generate oxidation damage to biomacromolecules in cells, abnormal activation of signal paths such as extracellular regulatory protein kinase 1/2 and nuclear factor kappa B is caused, and mitochondrial DNA damage and apoptosis are induced; can also stimulate the expression of matrix metalloproteinase, lead to the abnormal regulation of the processes of preventing differentiation and proliferation and damaging inflammation, and promote the degradation of collagen. In addition, ROS can also activate nuclear factor kB (NF-kB) signal channels, stimulate the transcription of genes such as proinflammatory cytokines (IL-1, TNF-alpha, IL-6) and the like, and cause skin tissue inflammatory reaction.

It is estimated that about 90% of the damage to the exposed skin is caused by UV irradiation, and long-term exposure to UV irradiation causes skin roughness, reddening, pigmentation, wrinkles deepening, thickening, etc., which affect beauty and beauty. Skin ages all the time, its elasticity slowly decreases, becomes thinner, fragile, dry and loose, and UV-induced skin damage is getting more and more attention. Therefore, the photo-aging problem has become the biggest problem threatening the health of skin, and is one of the hot spots in the research of skin care products.

The measures for preventing and treating skin photoaging mainly comprise the following steps: reducing skin exposure; reducing UV damage to the skin using sunscreen products; use of antioxidants for prophylaxis; non-steroidal drugs such as aspirin are used to delay or reduce the erythemal effect and treat inflammation caused by photodamage. However, the anti-photoaging effect of the products on the market is not ideal at present, and most of the products are added with synthetic chemical components, so that the skin care composition has a certain safety problem, and therefore, the skin care composition which is composed of pure natural plant extracts and can effectively resist photoaging and repair skin damage caused by UV (ultraviolet) in a multi-effect manner has a very important significance.

Disclosure of Invention

In view of the above-mentioned shortcomings, the present invention provides a composition containing an extract of hydrolyzed algae, which can protect cellular DNA from being damaged by ultraviolet rays, simultaneously protect against the oxidation of active oxygen free radicals caused by ultraviolet irradiation, reduce the formation of various active oxygen, effectively protect cells from being damaged by ultraviolet rays through the above two ways, improve the phenomena of rough skin, reddening, pigmentation, wrinkle deepening and thickening, etc. caused by photoaging, and make the skin more uniform, fine and glossy.

Therefore, the first technical scheme provided by the invention is as follows:

a composition containing a hydrolyzed algae extract comprises the following components in parts by weight: 4-12 parts of hydrolyzed algae extract, 3-9 parts of cassia wingnut leaf extract, 6-18 parts of active oxygen scavenging factor, 15-30 parts of polyalcohol, 15-25 parts of seawater and 20-40 parts of water; the active oxygen scavenging factor comprises litchi pericarp extract and Aralia elata leaf extract.

Further, in the above composition, the active oxygen scavenging factor further comprises an extract of a fermentation product of sphingomonas.

Further, in the composition, the weight ratio of the hydrolyzed algae extract to the cassia alata leaf extract is 2: 3;

furthermore, in the composition, the weight ratio of the litchi peel extract, the argania spinosa leaf extract and the sphingomonas fermentation product extract in the active oxygen scavenging factor is 3:1: 2.

Furthermore, in the composition, the polyalcohol is one or a combination of more of butanediol, glycerol and 1, 3-propanediol.

The second technical scheme provided by the invention is as follows:

the composition containing the hydrolyzed algae extract is applied to preparing cosmetics, wherein the cosmetics are one of water aqua, emulsion, essence, cream, gel and facial mask, and the mass percentage of the composition is 0.5-8%. The selection of other ingredients in the cosmetic and the preparation process may be carried out according to conventional procedures in the art at the time of manufacture.

Compared with the prior art, the invention has the following beneficial effects:

(1) the composition provided by the invention contains a cassia wingnut leaf extract and a hydrolyzed algae extract, wherein the cassia wingnut leaf extract is rich in K3OS, and can promote a cell DNA self-repair system, improve the micro surface of skin and prevent photoaging by preventing UVB from damaging cell nucleus DNA and UVA from damaging mitochondrial DNA; the hydrolyzed algae extract is from original blue microalgae, Iran mat blue algae, has strong photoprotection system of thioredoxin and biopterin, can protect mitochondrial membrane, protect cell DNA, prevent DNA damage and apoptosis, promote cell renewal and repair, improve cell activity, and delay cell death and aging process. The two components have synergistic effect, and can effectively protect cell DNA, improve cell activity, and resist cell damage caused by ultraviolet.

(2) The active oxygen scavenging factor in the composition comprises a litchi peel extract, an acanthus spinosus leaf extract and a sphingomonas fermentation product extract, and when the three are compounded in a certain proportion, the active oxygen caused by ultraviolet irradiation can be more effectively scavenged, so that the oxidative damage is reduced.

(3) The composition comprises a cassia wingnut leaf extract, a hydrolyzed algae extract, an active oxygen scavenging factor and the like, and the synergistic effect of various components can improve the cell activity, protect cell DNA from being damaged by ultraviolet rays, simultaneously resist the oxidation of active oxygen free radicals caused by ultraviolet irradiation, reduce the formation of various active oxygen, protect cells from being damaged by ultraviolet rays through the two ways, effectively improve the phenomena of rough skin, red swelling and hot pain, pigmentation, wrinkle deepening and thickening and the like caused by photoaging, and enable the skin to be more uniform, fine and glossy.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. For process parameters or conditions not specifically mentioned, it can be carried out with reference to conventional techniques.

The raw materials used in the invention are all obtained by a self-made mode or purchased from commercial channels. Wherein the extract of Cassia alata leaf, the extract of Acacia spinosa leaf and the extract of litchi pericarp are purchased from Xinan chemical trade company, Inc. in Foshan City, the extract of hydrolyzed algae is purchased from Yuyu biological technology (International) group, and the extract of sphingomonas fermentation product is purchased from Baihabo company, Inc. in Guangzhou City.

Example 1

The invention provides a composition containing an algae hydrolysis extract, which comprises the following components in parts by weight, based on 100g of the total weight: 10g of hydrolyzed algae extract, 7g of cassia wingnut leaf extract, 2g of litchi peel extract, 4g of acanthus leaf extract, 24g of butanediol, 25g of seawater and 28g of water.

Example 2

The invention provides a composition containing an algae hydrolysis extract, which comprises the following components in parts by weight, based on 100g of the total weight: 12g of hydrolyzed algae extract, 8g of cassia wing leaf extract, 3g of litchi peel extract, 7g of acanthus spinosus leaf extract, 30g of glycerol, 15g of seawater and 25g of water.

Example 3

The invention provides a composition containing an algae hydrolysis extract, which comprises the following components in parts by weight, based on 100g of the total weight: 5g of hydrolyzed algae extract, 5g of cassia wingnut leaf extract, 10g of litchi peel extract, 6g of argania spinosa leaf extract, 15g of 1, 3-propylene glycol, 19g of seawater and 40g of water.

Example 4

The invention provides a composition containing an algae hydrolysis extract, which comprises the following components in parts by weight, based on 100g of the total weight: 4g of hydrolyzed algae extract, 6g of cassia wingnut leaf extract, 10g of litchi peel extract, 6g of argania spinosa leaf extract, 15g of 1, 3-propylene glycol, 19g of seawater and 40g of water.

Example 5

The invention provides a composition containing an algae hydrolysis extract, which comprises the following components in parts by weight, based on 100g of the total weight: 9g of hydrolyzed algae extract, 3g of cassia wingnut leaf extract, 6g of litchi peel extract, 6g of argania spinosa leaf extract, 6g of sphingomonas fermentation product extract, 10g of butanediol, 18g of glycerol, 22g of seawater and 20g of water.

Example 6

The invention provides a composition containing a hydrolyzed algae extract, which comprises the following components in parts by weight based on 100g of the total weight: 6g of hydrolyzed algae extract, 9g of cassia winged bud leaf extract, 6g of litchi peel extract, 2g of argania spinosa leaf extract, 4g of sphingomonas fermentation product extract, 8g of butanediol, 5g of glycerol, 10g of 1, 3-propanediol, 20g of seawater and 30g of water.

Example 7

The invention provides a composition containing a hydrolyzed algae extract, which comprises the following components in parts by weight based on 100g of the total weight: 6g of hydrolyzed algae extract, 9g of cassia winged bud leaf extract, 4g of litchi peel extract, 6g of argania spinosa leaf extract, 2g of sphingomonas fermentation product extract, 8g of butanediol, 5g of glycerol, 10g of 1, 3-propanediol, 20g of seawater and 30g of water.

Comparative example 1

In contrast to example 4, the extract of the leaves of Cassia alata was replaced with the extract of the hydrolyzed algae in equal amounts, the other ingredients being identical.

Comparative example 2

Compared with example 6, the litchi pericarp extract was 12g, and contained no Aralia spinosa leaf extract and sphingomonas sp.

Comparative example 3

In comparison with example 6, the leaf extract of Aralia elata was 12g, and contained no litchi pericarp extract and sphingomonas ferment extract, and other components were the same.

Comparative example 4

Compared with example 6, the extract of the fermentation product of sphingomonas was 12g, and contained no litchi pericarp extract and no Aralia elata leaf extract, and other components were the same.

The composition containing the algae hydrolysate extract can be mixed and stirred uniformly in advance, and then directly added into cosmetics at low temperature, and can be prepared into skin care cosmetics such as aqua, emulsion, essence, cream, gel, facial mask and the like according to the conventional process and auxiliary materials for preparing the cosmetics, wherein the mass percentage of the composition is 0.5-8%. The selection of the other ingredients in the cosmetic product at the time of manufacture may be made according to conventional procedures in the art.

In order to better illustrate the advantages of the present invention, the experimental results of the technical solution provided by the present invention are given below:

first, cell experiment

Hacat cells in logarithmic growth phase were trypsinized into single cell suspension. Cells were seeded in 96-well plates (1X 10) prior to the experiment⁵One/well), continuously culturing for 24h, randomly dividing the cells into a blank control group, a UVA + UVB radiation group and a UVA + UVB + administration group, and arranging 3 multiple wells for each group of cells. The blank control group and the UVA + UVB irradiated group were added with DMEM medium, and the UVA + UVB + administered group were added with the compositions of examples 1-7 and comparative examples 1-4 diluted to 40. mu.g/mL with DMEM medium, respectively. After 24 hours of incubation, the cells were irradiated with broad-spectrum UVB (emission peak 313nm) at a dose of 30mJ/cm in a UVA + UVB irradiation group and a UVA + UVB + administration group²Simultaneously irradiating the cells with UVA (emission peak at 365nm) at a dose of 10J/cm²(ii) a The blank control group was covered with tinfoil paper and protected from light.

1. Cell activity assay: adding MTT (5mg/ml) solution into the treated cells, incubating for 4h, removing supernatant, adding DMSO, placing in a constant-temperature culture oscillator, oscillating for 10min, measuring the A value of each hole at 490nm with a microplate reader, and comparing the A value of each group of cells with the A value of the blank group of cells to obtain the cell viability percentage.

2. Detecting intracellular ROS levels: intracellular reactive oxygen species levels were detected using the ROS detection kit. Adding the cells washed by PBS buffer solution into DCFH-DA culture solution containing 10 mu mol/L, incubating for 20min at 37 ℃, washing for 3 times without serum to fully remove DCFH-DA which does not enter the cells, placing under a laser confocal microscope, selecting a 20 Xobjective lens and a 7% optical filter, wherein the wavelength of excitation light is 488nm, the emission wavelength is 525nm, selecting the optimal cell scanning parameters after pre-scanning, and locking the parameters. 3 visual fields are selected for each observation index, after 2 times of laser scanning, the average fluorescence intensity in cytoplasm and nucleus is recorded, and the collected experimental data is stored. In the experiment, the average fluorescence gray in cytoplasm and nucleus is used as an index for judging the activity of ROS, and 30 cells in 3 visual fields are selected for quantitative analysis of fluorescence gray.

3. DNA damage: the irradiated cells were discarded from the culture medium, washed with cold bath PBS 2-3 times, and then 0.5mL of cell lysate was added to each well to lyse the cells on ice for about 15 min. Then the lysate is centrifuged for 15min, and the supernatant is taken for single cell gel electrophoresis experiment. Cells were observed under a fluorescent microscope, and the tail length was analyzed using CASP image processing software, and the degree of damage to DNA was analyzed based on the measurement results.

The results of the above tests are shown in table 1.

TABLE 1

As can be seen from the results in table 1, UVB and UVA irradiation causes decreased cellular activity, increased ROS levels and DNA damage. However, the addition of the compositions of examples 1-7 of the present invention effectively increased cellular activity, decreased ROS levels, and reduced UV-induced cellular DNA damage. Comparing comparative example 1 with example 4, it is known that the cassia wingless cassia leaf extract and the hydrolyzed algae extract are synergistic, and can better improve the activity of cells and protect the cells from being damaged by ultraviolet rays. Compared with the comparative examples 2-4 and the example 6, the combination of the litchi pericarp extract, the argania spinosa leaf extract and the sphingomonas fermentation product extract can effectively eliminate active oxygen generated by ultraviolet irradiation, obviously reduce the damage of ultraviolet rays to cells, and has the effect after combination superior to the protection effect of the single extract and obvious synergistic effect.

Second, human body test

1. Test sample

The compositions of examples 3, 4, 6 and 7 of the present invention were added to the emulsion base in an amount of 4% to obtain corresponding cosmetics, and the emulsion base was used as a control.

The emulsion matrix was as follows:

phase A: 2g of isopropyl palmitate, 3g of caprylic/capric triglyceride, 0.8g of hydrogenated polyisobutene, 0.1g of propylparaben, 2g of cyclopentadecyldimethyl siloxane// cyclohexasiloxane, 0.5g of glyceryl stearate and 0.2g of bisabolol;

phase B: 0.2g of allantoin, 5g of glycerol, 0.02g of EDTA disodium, 0.3g of xanthan gum and water added to 100 g;

and C phase: cetearyl olive oleate// sorbitan olive oleate 1.5 g;

phase D: the composition of the example is 4g, a proper amount of preservative and a proper amount of essence.

The preparation method comprises the following steps: mixing the phase A materials, heating to 80 ℃, dissolving and transparent, and keeping the temperature for later use; adding water in the B-phase material into an emulsifying pot, heating to 90-95 ℃, preserving heat for 30 minutes, then sterilizing, adding the B-phase rest material, homogenizing uniformly, cooling to 75 ℃, adding the C-phase material, preserving heat, and stirring uniformly; filtering the prepared A-phase material, pumping into an emulsifying pot, homogenizing, emulsifying uniformly, stirring at 75 ℃, defoaming, and cooling completely; cooling to 40 ℃, adding the D-phase material, uniformly stirring, continuously cooling to 38 ℃, filtering and discharging after the plate checking is qualified.

2. Testing personnel

40 female patients were selected, aged 30-52 years, and the mean age was 38.5 + -5.07 years, and divided into 8 groups.

3. The test method comprises the following steps:

the test was conducted in summer, two bottles of the cosmetic were dispensed to each volunteer, and the volunteer was given only the emulsion base on the left face as a control and the cosmetic emulsion containing the composition of examples 3, 4, 6, and 7 of the present invention on the right face as a test group, respectively. The test part is prevented from contacting other external preparations, and is applied twice per day, about 0.5g each time, for 1 month. Volunteers were kept for 1 hour of outdoor activity between 8-11 am each day, and VISIA skin tester was used to examine the image information (including spots, wrinkles, texture, uv-pigmented spots, red areas) of the left and right faces of the subjects before and 1 month after using the product, and to perform automatic data analysis. The test results are shown in Table 2.

Each set of values (measurement data after use-measurement data before use)/measurement data before experiment × 100%.

TABLE 2

The experimental result shows that the face of all the volunteers applied with the cosmetics of the test group has no allergic symptoms such as erythema, edema, pimple and the like. The experimental results also show that spots, wrinkles, textures, ultraviolet spots and red areas are obviously reduced before and after the right face test after the test group is smeared, and the obvious difference is shown in table 2 compared with the control group before and after the test. The composition can effectively resist the phenomena of rough skin, reddening, pigmentation, wrinkle deepening and thickening and the like caused by ultraviolet irradiation, improves the smoothness of the skin, has the function of resisting photoaging, has good protective effect on skin cells, and is a very ideal cosmetic additive.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The composition containing the hydrolyzed algae extract is characterized by comprising the following components in parts by weight: 4-12 parts of hydrolyzed algae extract, 3-9 parts of cassia wingnut leaf extract, 6-18 parts of active oxygen scavenging factor, 15-30 parts of polyalcohol, 15-25 parts of seawater and 20-40 parts of water;

the active oxygen scavenging factor is litchi pericarp extract and Aralia elata leaf extract;

or the active oxygen scavenging factor is litchi pericarp extract, Aralia mandshurica leaf extract and sphingomonas ferment product extract.

2. The composition of claim 1, wherein the active oxygen scavenging factor comprises a weight ratio of litchi pericarp extract, Argania spinosa leaf extract, Sphingomonas sp.

3. The composition of claim 1, wherein the weight ratio of the hydrolyzed algae extract to the cassia wingless cassia leaf extract in the composition is 2: 3.

4. The composition according to claim 1, wherein the polyhydric alcohol is one or more of butylene glycol, glycerol and 1, 3-propylene glycol.

5. Use of the composition containing an extract of hydrolyzed algae according to any one of claims 1 to 4 for the preparation of cosmetics.

6. The use of the composition containing hydrolyzed algae extract according to claim 5, wherein the composition is present in an amount of 0.5 to 8% by weight of the cosmetic composition.

7. The use of the hydrolyzed algae extract-containing composition according to claim 5, wherein the cosmetic is one of a lotion, a milky lotion, an essence, a cream, a gel, and a mask.