KR20100021755A - Cosmetic composition for anti-aging of the skin - Google Patents

Abstract

PURPOSE: A cosmetic composition for improving anti-aging is provided to remove free radical or protect intracellular anti-oxidation. CONSTITUTION: An anti-aging cosmetic composition contains 0.01-90 weight% of blueberry, raspberry, and punica granatum L. extract. A method for extracting blueberry, raspberry, and punica granatum L. comprises: a step of adding the blueberry, raspberry, and punica granatum L. in flask; and a step of immersing with 2L of butylenes glycol for three days and filtering remove residue. The cosmetic composition is used in the form of emulsion, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, or lotion.

Description

Skin cosmetic composition having the effect of preventing skin aging {Cosmetic composition for anti-aging of the skin}

The present invention relates to a cosmetic composition that can improve skin aging caused by aging, and more particularly, blueberry, raspberry, and pomegranate excellent in free radical scavenging ability or intracellular antioxidant protection. The present invention relates to a cosmetic composition having an excellent effect of preventing and delaying skin aging by containing a super fruit complex containing an extract of (punica granatum L.).

The relationship between aging and free radicals is well known through many studies, and the fact that free radicals occur during normal metabolic processes causes oxidative damage to biological components and these damages accumulate and eventually lead to aging and death. This is known. Free radicals produced during normal metabolism include antioxidant enzymes such as superoxide dismutase or catalase, glutathione, ascorbic acid (vitamin C), and alphatocopherol. (α-tocopherol, vitamin E) or antioxidant defense factors such as ubiquinone (ubiquinone) is being removed, but not completely removed. Free radicals, which have not been removed, accumulate in cells and tissues in large amounts, damaging DNA and proteins in the cells, leading to genetic diseases such as cancer, Down syndrome, Alzheimer's disease, etc., as well as progressing aging by destroying cells and tissues.

In addition, since the skin is exposed to the atmosphere, not only intrinsic aging but also photoaging caused by daylight and ultraviolet rays is much progressed. Such sunlight and ultraviolet rays generate a lot of free radicals when they come in direct contact with the skin, and the antioxidant defenses of the skin are damaged by these free radicals, thereby increasing wrinkles and relaxing the skin, thereby accelerating skin aging.

On the other hand, most of the studies to date on antioxidants have been performed in vitro using 1,1-diphenyl-2-picryl hydrazyl (1,1-diphenyl-2-picryl hydrazyl, DPPH). in vitro ) and free radical scavenging ability, and in vivo to measure the actual protective effect by measuring the defense factor activity of the antioxidant system present in the skin cells or skin ( in In vivo testing has not reached the stage yet.

Accordingly, the present inventors protect antioxidant activities such as superoxide dismutase, catalase and glutathione in skin cells that are known to decrease their activity by ultraviolet rays as well as free radical scavenging and inhibit damage. Blueberries, raspberries and pomegranate extracts (Super fruit complex), which are known to have excellent activity, have been found to have an excellent effect when mixed and completed the present invention.

Accordingly, an object of the present invention is to provide an antioxidant and anti-aging skin cosmetic composition containing blueberries, raspberries and pomegranate extracts having free radical scavenging ability or intracellular antioxidant protection.

In order to achieve the above object, the present invention provides a cosmetic composition for anti-aging, characterized in that it contains blueberry, raspberry and pomegranate extract together.

Cosmetic composition of the present invention containing a super fruit complex (super fruit complex) complex of blueberry, raspberry, pomegranate extract is excellent in free radical scavenging or intracellular antioxidant protection effect, cosmetics having antioxidant and anti-aging efficacy It is very useful to make.

The present invention relates to a skin cosmetic composition having free radical scavenging ability or intracellular antioxidant protection, and comprises a super fruit complex composed of blueberry, raspberry and pomegranate extract.

Hereinafter, the present invention will be described in more detail.

Blueberry, raspberry and pomegranate extract contained in the super fruit complex of the present invention are as follows.

Blueberry extract is Vaccinium , a shrub of the dicotyledon tree Azalea. It is preferable to extract from the fruit of angustifolium ). Blueberry is a fruit belonging to the berry group together with strawberry, blackberry, bilberry, and cranberry, and contains a large amount of effective substances useful for the physiological function of the human body such as vitamin A, vitamin C, niacin, anthocyanin pigment, potassium and calcium. It contains.

Raspberry (raspberry) extract is preferably used to extract from the fruit of Rubus idaeus , a dicotyledon rose rosaceae raspberry fruit tree. Raspberries are not only rich in fiber, but also contain large amounts of effective substances that are useful for the physiological functions of the body, such as vitamin C and manganese, vitamins A, B, calcium, and potassium.

Pomegranate ( Punica) granatum L.) is preferably extracted from the fruit or leaves of the pomegranate tree, which is a broad-leaved large-key tree. Pomegranate contains abundant minerals and vitamins, and minerals include sodium, calcium, phosphorus, magnesium, zinc, manganese and iron, as well as proteins, carbohydrates and cyamines (vitamin B1) and riboflavin (vitamin B2). ), Niacin, alkaloids, percelene and beruic acid, uric acid, etc.

Blueberry, raspberry, and pomegranate extract provided by the present invention can be extracted by a conventional extraction method, and the type and content of the extraction solvent can also be optionally selected according to the purpose .

The cosmetic composition provided by the present invention contains a super fruit complex which is a complex of the blueberry, raspberry, and pomegranate extract as described above. The super fruit complex according to the present invention contains blueberry, raspberry and pomegranate extract preferably in a ratio of 1: 1: 1 in a combination based on scientific basic data on the skin, but the component ratio of the extract is limited thereto. It is not. As such, when the extracts are used in combination, the free radical scavenging ability is excellent, and the long-term efficacy can be substantially realized in terms of skin aging and antioxidant. In addition, the cosmetic composition of the present invention contains the super fruit complex in an amount of 0.001 to 90% by weight based on the total weight of the composition. If the super fruit complex composition is less than 0.001% by weight can not be expected to have a desirable effect on the anti-aging and antioxidant, if more than 90% by weight in cosmetic composition is low in appearance or economic efficiency.

The cosmetic composition using the same may be used for skin antioxidant and anti-aging cosmetics, and the formulation is not particularly limited, and for example, latex, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion Or a skin adhesive type cosmetic or transdermal dosage form.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited thereto.

Preparation Example 1 Preparation of Blueberry, Raspberry, and Pomegranate Extracts

Blueberries, raspberries, and pomegranates were purchased from the market, and 100 g of dry weight was added to each flask, followed by extraction with 2 l of butylene glycol for 3 days, followed by filtration to remove debris to prepare blueberry, raspberry, and pomegranate extracts. It was.

Experimental Example 1 Free Radical Scavenging Activity Measurement Experiment of Super Fruit Complex

In order to examine the effect of free radical scavenging activity of the super fruit complex, a blueberry extract: raspberry extract: pomegranate extract prepared in Preparation Example 1 was prepared with a super fruit complex having a ratio of 1: 1: 1. The scavenging activity was examined. However, the usable range of each component is not limited only to this ratio. In addition, the radical scavenging activity of each blueberry, raspberry and pomegranate extract was compared.

The measurement was performed by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and the reagent was prepared by the US Sigma Co., Ltd. (St. Louis, MO, USA). Since the reagent is present as a relatively stable free radical, it is the first in vitro method to confirm the free radical scavenging action ( in in vitro ).

The concentration of each extract and super fruit complex used for the measurement of DPPH free radical scavenging activity was 1% by volume, 0.1% by volume, and 0.01% by volume, respectively. Extracts of the above concentrations were placed in 96 well plates, respectively, to which DPPH prepared in 100 μM ethanol solution was added so that the total volume of the solution was 200 μl. This was allowed to stand at 37 ° C. for 30 minutes, and then absorbance was measured using a 520 nm ELISA reader. Free radical scavenging activity (%) was calculated by Equation 1 below, and the results are shown in Table 1 below.

Free radical scavenging activity (%) = 100- (B / A) * 100

A: absorbance of control wells not treated with the extract of the present invention

B: Absorbance of Experimental Wells Treated with Extracts of the Invention

content Vitamin C Blueberry Extract Raspberry extract Pomegranate extract Super Fruit Complex 50 ppm 79.1 - - - - 1% by volume - 82.7 79.9 71.2 92.2 0.1 volume% - 59.3 51.6 35.6 68.3 0.01 volume% - 30.1 22.0 13.8 40.1

As shown in the results of Table 1, the super fruit complex, which is a complex of blueberry, raspberry, and pomegranate extract, has excellent free radical scavenging activity, such as vitamin C, when blueberry, raspberry, and pomegranate extracts are used alone. It can be seen that the free radical scavenging activity is more excellent. It can also be seen that this free radical scavenging activity is concentration dependent.

Experimental Example 2 Measurement of Antioxidant Protective Effects of Super Fruit Complex on Human Skin Cell Lines

In this experiment, as a cell line, Dr. Human keratinocytes HaCaT cell line (Human keratinocytes HaCaT cell line) sold from Fusenig was used. After dispensing the cell lines into ^{six 6.0} × ¹⁰ cells in a 150 × 150 culture plate and incubating for one day, the cells were cultured with DMEM (Dulbeco's modified eagle's medium, GIBCO BRL, Life Technologues) containing 10 volumes of fetal bovine serum (FBS). four (社)) to blueberries, raspberries, a complex of the concentration of super fruit complex of pomegranate extract 1% by volume, 0.1% by volume, 0.01 dispensed in each treated culture medium to a volume% and 37 ℃, 5% CO ₂ the medium Incubated for 1 day under conditions. 1X phosphate buffer (PBS, phosphate buffered saline, Sigma, USA, St. Louis, MO, USA) was used for washing after incubation. After incubation, UV light was treated with an ultraviolet B (UVB) lamp (Model: F15T8, UVB 15W, Sankyo Dennki Co., Japan, Japan) at an intensity of 30 mJ / cm 2, at 37 ° C., 5% CO _2. The cells were harvested for one day, harvested, and used to measure antioxidant defenses. Vitamin C was used as a positive control to compare the results, the concentration of vitamin C was 10μM.

Superoxide dismutase (SOD), catalase (catalase) and glutathione (GSH) were measured as antioxidant defense factors, respectively. Superoxide dismutase is described by McCord's method [ J. Biol . Chem . (1969) 244: 6049-6055], and catalase was determined by Budhuin's method [ Biochem . J. (1964) 92: 179-184], and glutathione was measured by Akerboom's method [ Methods]. in Enzymology (1981) 77: 373-382].

The activity of the intracellular antioxidant system was expressed as the activity compared with this value in terms of the activity of the control group not irradiated with UVB and calculated by Equation 2 below, the results are shown in Table 2.

% Activity of intracellular antioxidant system = (B / A) * 100

A: activity of the control group

B: Activity of UVB treated group and each experimental group

Experimental group content Superoxide Dismutase Catalase Glutathione Control 100 100 100 UVB treatment group 54.9 73.2 62.8 Vitamin c 10 μM 81.2 84.9 83.7 Blueberry Extract 1% by volume 82.9 85.1 84.3 0.1 volume% 82.1 76.9 83.1 0.01 volume% 72.9 73.9 66.9 Raspberry extract 1% by volume 83.1 77.2 79.6 0.1 volume% 83.9 76.0 83.4 0.01 volume% 60.9 74.8 75.0 Pomegranate extract 1% by volume 75.9 76.7 76.0 0.1 volume% 71.5 76.8 76.9 0.01 volume% 61.3 73.3 68.0 Super Fruit Complex 1% by volume 90.5 92.1 91.0 0.1 volume% 88.9 85.7 86.4 0.01 volume% 81.8 79.0 77.1 The above data is an average of four measurements.

In addition, the protective effect of the superoxide complex (blueberry, raspberry and pomegranate extract) on the intracellular antioxidant system of the experimental group treated with the UVB-irradiated group was shown to protect the activity of the superoxide dismutase (SOD). , It was measured by dividing the activity protective effect of catalase and the active protective effect of reduced glutathione, the measurement results are expressed in the following formula (3), and the results are shown in Figures 1, 2 and 3.

Intracellular Antioxidant Protection Effect (%) = [(C-B) / (A-B)] * 100

A: activity of the control group

B: activity of UVB treatment group

C: activity of each experimental group

As can be seen from Table 2 and FIGS. 1 to 3, the superfruit complex, which is a complex of blueberry, raspberry and pomegranate extract, protects the activity of antioxidant defenses reduced by ultraviolet rays, as is well known vitamin C. It can be expected to show antioxidant efficacy and further anti-aging. In addition, it can be seen that the super fruit complex, which is a complex of blueberry, raspberry, and pomegranate extract, has an excellent antioxidant protection effect when each extract is used alone.

Experimental Example 3 Measurement of Antioxidant Protective Effects of Super Fruit Complex on Hairless Mouse Skin

Using a 10-week-old hairless mouse, an experiment was conducted to investigate the antioxidant effect of the super fruit complex on the skin's antioxidant activity against the deactivation of antioxidant defense factors in the skin irradiated with ultraviolet (UVB).

As shown in the composition of Table 3, an emulsion (Example 1) containing a super fruit complex was prepared and used as a sample, and an emulsion containing 5% by weight of vitamin C (Comparative Example 2) and blue for comparing the results. Emulsions containing 1% by weight of berry, raspberry, and pomegranate extracts (Comparative Examples 3, 4, 5) and emulsions containing neither blueberries, raspberries, pomegranates, vitamin C, or super fruit complexes (Comparative Example 1 ) Was prepared.

division Raw material name Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Example 1 Oily ingredients Stearic acid 1.0 1.0 1.0 1.0 1.0 1.0 Cetearyl Alcohol 0.2 0.2 0.2 0.2 0.2 0.2 Hydrogenated Oil 0.7 0.7 0.7 0.7 0.7 0.7 Lipophilic glyceryl stearate 0.5 0.5 0.5 0.5 0.5 0.5 Hydrogenated Lecithin / C12-16 Alcohol / Palmetic Acid 1.0 1.0 1.0 1.0 1.0 1.0 antiseptic 0.3 0.3 0.3 0.3 0.3 0.3 Polydecene 4.0 4.0 4.0 4.0 4.0 4.0 Meadowfoam seed oil 1.0 1.0 1.0 1.0 1.0 1.0 Pentaerythritol tetraisostearate 3.0 3.0 3.0 3.0 3.0 3.0 Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 Cetyloctanoate 2.0 2.0 2.0 2.0 2.0 2.0 Decyl cocoate 1.0 1.0 1.0 1.0 1.0 1.0 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 Water component Purified water To 100 To 100 To 100 To 100 To 100 To 100 Butylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Disodium ID 0.02 0.02 0.02 0.02 0.02 0.02 Tee 0.15 0.15 0.15 0.15 0.15 0.15 antiseptic 0.2 0.2 0.2 0.2 0.2 0.2 Vitamin c - 5.0 Blueberry Extract 1.0 Raspberry extract 1.0 Pomegranate Extract 1.0 Super Fruit Complex (Blueberry Extract: Raspberry Extract: Pomegranate Extract = 1: 1: 1) - - - - - 1.0

<< manufacturing method of an Example and a comparative example >>

(1) The oil phase components were heated (70 to 75 ° C) and mixed uniformly.

(2) The aqueous phase component was heated (70 to 75 ° C.) to be uniformly dissolved and mixed.

(3) In (2), (1) and (1) were added under stirring while maintaining the temperature (70-75 ° C) to form an emulsion, and cooled to 28-30 ° C.

The antioxidant protection effect of Example 1 and Comparative Examples 1-5 manufactured by the above method was measured by the following method.

The emulsions of Example 1 and Comparative Examples 1 to 5 were applied to the back of the hairless mice once a day for 4 days. The amount of ultraviolet light B irradiated during the test period was 400mJ / cm 2 using Waldmann UV800 (Herbert Waldmann GmbH & E Phillips TL / 12 lamp emitting 280-305nm). The back skin of the hairless mouse was biopsied and used for analysis, and the analysis items were the same as described in Experimental Example 2. The results were calculated by the equations (2) and (3) described in Experimental Example 2. The results of the activity of the intracellular antioxidant system are shown in Table 4 below, and the protective effect of the superoxide dismutase (SOD), the protective activity of the catalase, and the reduced glutathione activity of the intracellular antioxidant system. Measured by dividing effect, the results are shown in Figures 4, 5 and 6.

Experimental group and content Superoxide Dismutase Catalase Glutathione Control 100 100 100 Comparative Example 1 UVB treatment group 76.2 78.6 74 Comparative Example 2 Vitamin C 5.0 wt% 86.1 88 89.9 Comparative Example 3 Blueberry Extract 1.0 wt% 88.9 88.9 88.9 Comparative Example 4 Raspberry Extract 1.0 wt% 84.7 87.3 83 Comparative Example 5 Pomegranate Extract 1.0% by weight 82.5 83.9 78.6 Example 1 Super Fruit Complex 1.0 wt% 94.1 92.2 93.7 The above data is an average of four measurements.

As can be seen from Table 4 and Figures 4 to 6, the superfruit complex, which is a complex of blueberry, raspberry and pomegranate extract, has an activity of antioxidant defenses reduced by ultraviolet rays, such as vitamin C, which is well known for its antioxidant effect. It is expected to be able to show anti-oxidant effect by protecting and further show anti-aging action. In addition, it can be seen that the superfruit complex, which is a complex of blueberry, raspberry, and pomegranate extract, has significantly improved antioxidant efficacy than when each extract is used alone.

Experimental Example 4 Confirmation of Skin Wrinkle Improvement Effect

The skin wrinkle improvement effects of Example 1 and Comparative Examples 1 to 5 according to the present invention were evaluated for humans. Eighty females in their 30s to 40s were divided into six groups of 15 people, and the emulsions of Examples 1 and Comparative Examples 1 to 5 were applied to the designated eye areas twice daily for 6 weeks each morning and evening. After 6 weeks, the wrinkles around the face were collected with a silicone resin copy (replica) before and after 6 weeks of use, and the skin fine wrinkles and skin image analyzer were used to compare the condition of fine wrinkles. The results are shown in Table 5 below.

Experimental group Average fine wrinkle depth Wrinkle Reduction (%) Before use 6 weeks after use Comparative Example 1 - 20.6 20.1 2.43 Comparative Example 2 Vitamin C 5.0 wt% 20.8 17.6 15.38 Comparative Example 3 Blueberry Extract 1.0 wt% 20.7 17.3 16.43 Comparative Example 4 Raspberry Extract 1.0 wt% 20.9 18.1 13.40 Comparative Example 5 Pomegranate Extract 1.0% by weight 20.8 18.9 9.13 Example 1 Super Fruit Complex 1.0 wt% 20.7 15.5 25.12

As can be seen in Table 5, when using Example 1 containing a super fruit complex, a complex of blueberry, raspberry and pomegranate extract, the skin wrinkle improvement effect was the best in the skin around the eye of the experimenter, When the blueberry, raspberry, pomegranate extract was used alone, it was found that the wrinkle improvement effect is superior.

Figure 1 shows the activity protective effect of SOD against human skin cell lines of the super fruit complex.

Figure 2 shows the activity protective effect of catalase on human skin cell lines of the Super Fruit Complex.

Figure 3 shows the protective effect of GSH on human skin cell lines of the Super Fruit Complex.

Figure 4 shows the protective effect of SOD on the hairless mouse skin of the Super Fruit Complex.

Figure 5 shows the protective activity of catalase on hairless mouse skin of the Super Fruit Complex.

Figure 6 shows the protective effect of GSH on hairless mouse skin of the Super Fruit Complex.

Claims (6)

Anti-aging cosmetic composition characterized in that it contains blueberry, raspberry and pomegranate extract together. The anti-aging cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. The anti-aging cosmetic composition according to claim 1, wherein the composition eliminates free radicals. According to claim 1, wherein the composition is an anti-aging cosmetic composition, characterized in that to activate the intracellular antioxidant system. According to claim 1, wherein the composition is an anti-aging cosmetic composition, characterized in that to protect the antioxidant protective activity. According to claim 1, wherein the anti-aging cosmetic composition, characterized in that the composition has an anti-wrinkle action.

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