CN111557875A - Skin care composition for resisting light pollution and light injury and preparation method and application thereof - Google Patents
Skin care composition for resisting light pollution and light injury and preparation method and application thereof Download PDFInfo
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- CN111557875A CN111557875A CN202010490105.5A CN202010490105A CN111557875A CN 111557875 A CN111557875 A CN 111557875A CN 202010490105 A CN202010490105 A CN 202010490105A CN 111557875 A CN111557875 A CN 111557875A
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
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- A61K8/4953—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
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Abstract
The application discloses a skin care composition for resisting light pollution and light damage, which comprises: ergothioneine Armillaria mellea extract, tetrahydro-methylpyrimidine carboxylic acid, Ecliptae herba extract and hyaluronic acid. The application also discloses a preparation method of the light pollution and light damage resistant skin care composition, a skin care product and application of the phellodendron amurense bark extract in the light pollution and light damage resistant composition. The skin care composition provided by the application has strong ultraviolet and blue light filtering capacity, can effectively prevent ultraviolet and blue light from irradiating the skin, and reduces the damage of the ultraviolet and blue light to the skin; the composition provided by the application can also inhibit the oxidative stress reaction of skin cells caused by ultraviolet and blue light irradiation, and inhibit and eliminate the generation of ROS; the skin composition provided by the application can also improve the cell hypoxia state of cells after being irradiated by ultraviolet rays and blue light, so that the cells can recover the normal metabolism function, and the skin composition has the repairing effect after photodamage.
Description
Technical Field
The application relates to the technical field of skin care products, in particular to a skin care composition with light pollution and light damage resistance, and a preparation method and application thereof.
Background
Light pollution is a phenomenon in which excessive or inappropriate light radiation generated in modern society has an adverse effect on human life and production environment. Typically including photopic pollution, artificial daytime pollution and glowing pollution. Sometimes people are classified into infrared light pollution, ultraviolet light pollution, laser pollution, visible light pollution, and the like according to the wavelength of light. At present, more and more attention is paid to the damage of ultraviolet rays and blue light to human skin, the ultraviolet rays and the blue light can enable skin cells to generate excessive active oxygen, the oxidation degree in vivo exceeds the removal rate of oxides, the oxidation and antioxidation systems are unbalanced, and the excessive active oxygen presses the cells, so that DNA damage, lipid peroxidation, collagen structure damage and mitochondrial function damage can be caused. These injuries may ultimately cause skin problems or skin diseases such as photoaging, contact dermatitis, atopic dermatitis, skin cancer, seborrhea, and the like.
To combat the damage to the skin caused by the ultraviolet rays in the sun, various sunscreen products have been developed and marketed, including physical barriers and chemical absorbing substances; in the aspect of resisting blue light, people also start to consciously research related blue light protection.
The Chinese patent application with publication number CN 110236965A discloses a blue light resisting skin care cosmetic and a preparation method thereof, wherein the composition comprises carotene, a camellia extract and a saffron extract, and the composition has strong blue light filtering capability, can block the damage of blue light to the skin, can remove free radicals generated by blue light irradiation, and can reduce the damage of the free radicals to the skin.
Chinese patent application publication No. CN 106727027a discloses a composition for resisting blue light, resisting oxidation, scavenging free radicals, skin care product and preparation method thereof, and the patent publication shows that the composition of marigold flower extract and safflower seed oil has the ability of filtering blue light, and the main active ingredient for resisting blue light is lutein.
The Chinese patent application with the publication number of CN 109464308A discloses an essential oil composition with blue light resisting and skin caring effects and an application thereof, the composition in the patent is orange flower essential oil, pomegranate essential oil, ginger essential oil, lavender essential oil and the balance of base oil, and the effects of absorbing blue light, activating blood, whitening, resisting free radicals and moisturizing of the essential oil composition are utilized to comprehensively resist damage to skin.
Disclosure of Invention
In view of the above, the present application provides a skin care composition, a preparation method and an application of the skin care composition, and a skin care product. The skin care composition provided by the application has strong ultraviolet and blue light filtering capacity, can effectively prevent ultraviolet and blue light from irradiating the skin, and reduces the damage of the ultraviolet and blue light to the skin; the composition provided by the application can also inhibit the oxidative stress reaction of skin cells caused by ultraviolet and blue light irradiation, and inhibit and eliminate the generation of ROS; the skin care composition provided by the application can also improve the cell hypoxia state of cells after being irradiated by ultraviolet rays and blue light, so that the cells can recover the normal metabolism function, and the skin repair effect after photodamage is promoted.
To achieve the above objects, according to one aspect of the present application, there is provided a skin care composition.
The present application provides the following technical solutions.
1. A photostain and photodamage resistant skin care composition comprising:
an extract of Armillaria Clavipitana,
Tetrahydro-methyl-pyrimidine-carboxylic acid,
Tropaeolum majus extract and
a hyaluronic acid-like substance.
2. The skin care composition of item 1, further comprising: alfalfa extract.
3. The skin care composition of item 2, wherein the skin care composition further comprises: cortex Phellodendri extract.
4. The skin care composition according to item 3, characterized by comprising the following 100 parts by weight relative to the total weight of the composition:
the ergothioneine Armillaria mellea extract is: 0.1 to 10 portions;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 1-20 parts;
the tropaeolum majus extract is as follows: 0.5 to 15 portions;
the alfalfa extract is: 1-20 parts;
the phellodendron amurense bark extract is as follows: 0-5 parts;
the hyaluronic acid substances are: 2.5 to 20 portions.
5. The composition according to item 3, characterized by comprising the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.5 to 5 parts;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 5-15 parts;
the tropaeolum majus extract is as follows: 2.5 to 10 portions;
the alfalfa extract is: 5-15 parts;
the phellodendron amurense bark extract is as follows: 2-5 parts;
the hyaluronic acid substances are: 5 to 15 portions.
6. A skin care composition according to any of claims 1 to 5 wherein the hyaluronic acid-like substance is hyaluronic acid and/or a hyaluronate salt;
the hyaluronic acid salt is selected from one or more of hyaluronic acid sodium salt, hyaluronic acid potassium salt and hyaluronic acid zinc salt, and preferably hyaluronic acid sodium salt.
7. Skin care composition according to any of items 1 to 5, characterized in that the molecular weight of the hyaluronic acid-like substance is between 0.1wDa and 5wDa, preferably between 0.1wDa and 1 wDa.
8. The skin care composition according to any one of claims 1 to 5, further comprising a humectant selected from one or more of trehalose, sodium polyglutamate, and tremella polysaccharide.
9. The skin care composition according to any one of claims 1 to 5, further comprising water and a preservative selected from one or more of phenoxyethanol, ethylhexyl glycerin, pentanediol, butanediol, and methylparaben.
10. A method of preparing a photocontamination and photodamage resistant skin care composition comprising the steps of:
uniformly mixing ergothioneine Armillaria matsutake extract, tetrahydro-methylpyrimidine carboxylic acid, Ecliptae herba extract and hyaluronic acid substances to obtain a mixture I, adding water into the mixture, and stirring to dissolve to obtain the skin care composition.
11. The method of claim 10, wherein the phellodendron amurense bark extract and the alfalfa extract are added to the first mixture and mixed uniformly to obtain a second mixture.
12. The method of claim 11, comprising the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.1 to 10 portions;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 1-20 parts;
the tropaeolum majus extract is as follows: 0.5 to 15 portions;
the alfalfa extract is: 1-20 parts;
the phellodendron amurense bark extract is as follows: 0-5 parts;
the hyaluronic acid substances are: 2.5 to 20 portions.
13. The method of claim 11, comprising the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.5 to 5 parts;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 5-15 parts;
the tropaeolum majus extract is as follows: 2.5 to 10 portions;
the alfalfa extract is: 5-15 parts;
the phellodendron amurense bark extract is as follows: 2-5 parts;
the hyaluronic acid substances are: 5 to 15 portions.
14. The production method according to any one of claims 11 to 13, characterized in that a preservative and a humectant are added to the second mixture before the second mixture is added with water.
15. The method according to item 14, wherein the humectant is one or more selected from trehalose, sodium polyglutamate, and tremella polysaccharide.
16. The method according to claim 14, wherein the preservative is one or more selected from phenoxyethanol, ethylhexylglycerin, pentanediol, butanediol, and methylparaben.
17. A skin care product comprising the skin care composition according to any one of claims 3 to 11.
18. The skin care product of claim 17, wherein the skin care composition is present in the skin care product in an amount of from 0.5% to 20% by weight.
19. The skin care product of claim 17, wherein the skin care composition is present in the skin care product in an amount of from 1% to 8% by weight.
20. The skin care product of item 17, wherein the skin care composition is present in the skin care product at a weight percentage of 5%.
21. Use of a skin care composition according to any of claims 1 to 9 in cosmetics, skin care products, toiletries.
22. Use of cortex Phellodendri extract in anti-photopollution and photodamage composition is provided.
23. The use according to item 22, wherein the anti-photocontamination, photodamage composition further comprises alfalfa extract.
The skin care composition capable of resisting light pollution and light damage has strong ultraviolet and blue light filtering capacity, can effectively prevent ultraviolet and blue light from irradiating the skin, and reduces damage of the ultraviolet and blue light to the skin.
The anti-light pollution and anti-light injury skin care composition provided by the application can also inhibit the oxidative stress reaction of skin cells caused by ultraviolet and blue light irradiation, and inhibit and eliminate the generation of ROS.
The skin care composition capable of resisting light pollution and light injury provided by the application can also improve the cell hypoxia state of cells after being irradiated by ultraviolet rays and blue light, so that the cells can recover the normal metabolism function, and the skin repair effect after light injury is promoted.
The components of the anti-light pollution and anti-light injury skin care composition provided by the application are good in thermal stability, and the raw materials are all plant extraction or biological fermentation sources, so that the skin care composition belongs to safe natural raw materials, has no stimulation to skin, and is suitable for any skin type skin including sensitive skin.
Drawings
Fig. 1 is a graph of the uv-vis absorption spectrum of the anti-photocontamination, photodamage skin care compositions of the present application.
Detailed Description
The following description of the exemplary embodiments of the present application, including various details of the embodiments of the present application to assist in understanding, should be taken as exemplary only. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the present application. Also, descriptions of well-known functions and constructions are omitted in the following description for clarity and conciseness.
The application provides application of phellodendron amurense bark extract in a composition for resisting light pollution and light damage.
The anti-photocontamination and photodamage composition further comprises alfalfa extract.
Light pollution is a phenomenon in which excessive or inappropriate light radiation generated in modern society has an adverse effect on human life and production environment. Typically including photopic pollution, artificial daytime pollution and glowing pollution. Sometimes people are classified into infrared light pollution, ultraviolet light pollution, laser pollution, visible light pollution, and the like according to the wavelength of light. The ultraviolet light is invisible light in a high-energy region of sunlight, and can be divided into three regions according to different wavelengths of the ultraviolet light, namely long-wave ultraviolet light (UVA, the wavelength is 320-plus-400 nm), medium-wave ultraviolet light (UVB, the wavelength is 280-plus-320 nm) and short-wave ultraviolet light (UVC, the wavelength is 200-plus-280 nm), wherein the ultraviolet light can penetrate through the epidermis of the skin and directly reach the superficial dermis, the ultraviolet light in the sunlight can damage the skin, and the ultraviolet light can cause skin erythema, skin tanning, skin photoaging, photosensitive skin diseases and the like. Blue-violet light (hereinafter referred to as blue light), also called high-energy visible light, is a light wave with the shortest wavelength and the highest energy in the visible spectrum, and the wavelength is within the range of 380nm to 500 nm. With the coming of the digital era, the frequency of using computers, mobile phones, televisions and tablet computers is higher and longer, and the application range of LEDs is wider and wider with the enhancement of energy-saving awareness, the light emitted by the light emitting diodes of these electronic products is generally in the blue light band, and people inevitably expose to the environment polluted by blue light every day, and the time of exposing to blue light every day is counted for at least 8 hours. The blue light pollution has great harm to retina and skin, the blue light has larger penetrating energy than ultraviolet rays, and the blue light can penetrate through epidermis and dermis of the skin and reach deep dermis of the skin or even reach a basal layer. A large number of experimental studies show that blue light, like ultraviolet light, can induce skin cells to generate oxidative stress, increase active oxygen in the cells, lipid peroxidation, DNA damage, melanin increase and the like, so that the skin cells are damaged, the cell metabolism is reduced, the barrier function of the skin is damaged, and the skin becomes dry, dark and dull in appearance, even loose and premature in aging and the like.
The skin care composition of the prior patent has certain limitation on resisting the damage of blue light to skin, firstly, the blue light resisting effect is not really effective enough, the stability of active substances is poor, the active substances are easy to lose activity in storage and transportation to reduce the blue light resisting effect, in addition, some blue light resisting components are only used for filtering the blue light, and the repairing effect after the damage caused by the ultraviolet light and the blue light is lacked; secondly, essential oil compositions are limited in the form of skin care products; in addition, the problem of photodamage that causes skin cells to become hypoxic is ignored by most people. Therefore, in view of the above problems, the present invention provides a skin care composition with anti-light pollution and anti-damage effects. The skin care composition with the effects of resisting light pollution and light injury can effectively filter blue light, inhibit oxidative stress reaction of cells, reduce damage to cells, improve the anoxic state of the cells after the irradiation of ultraviolet rays and blue light, recover the normal metabolism function of the cells and promote the skin repair effect.
Cortex Phellodendri (Phellodendron amurense) bark extract is yellow powder extract product of bark of cortex Phellodendri (Phellodendron amurense) belonging to Rutaceae. Mainly contains berberine, and contains palmatine, phellodendrine, dauricine, sinomenine, phellodendron bark, etc. Can promote the generation of collagen, and has the function of anti-aging by combining the oxidation resistance; can inhibit hair growth, and can be used for treating skin hirsutism in combination with aromatase activation; has anti-inflammatory and odor inhibiting effects. The phellodendron amurense bark extract can also absorb light within the range of 300-480 nm, and the aqueous solution containing the phellodendron amurense bark extract can effectively filter blue light; and is more stable than carotene and lutein, and has good stability under light and heat.
The alfalfa (Medicago sativa) extract contains chemical components such as flavone, triterpene, alkaloid, coumarin, protein and polysaccharide, has biological activities in multiple aspects such as antibiosis, antioxidation, immunoregulation and cholesterol reduction, can effectively filter the spectrum within the range of 280nm-500nm, but has better light and heat stability compared with carotene and lutein, so the alfalfa (Medicago sativa) extract has more advantages when being applied to skin care products with ultraviolet and blue light pollution resistance.
The present application provides a skin care composition that is resistant to light contamination, light damage, comprising: ergothioneine Armillaria mellea extract, tetrahydro-methylpyrimidine carboxylic acid, Ecliptae herba extract and hyaluronic acid.
In the present application, the skin care composition further comprises phellodendron amurense bark extract.
In the present application, the skin care composition further comprises alfalfa extract.
The ergothioneine Armillaria matsutake extract is a natural antioxidant, is safe and nontoxic, and has multiple physiological functions of removing toxic substances, maintaining DNA biosynthesis and the like. Ergothioneine is a natural sunlight covering substance, can block ultraviolet ray from invading skin, can repair injured collagen and elastic fiber, shrink pores, play a role in preventing sunlight, and can also effectively remove high-concentration free radicals in human bodies, remove color spots, whiten skin, delay aging and prevent various diseases. The main role of antioxidation is to slow down the aging rate of the human body, which is also the most core function of ergothioneine, but the human body cannot synthesize the ergothioneine by itself, so the ergothioneine must be obtained from the outside. The natural antioxidant is taken by people through daily diet to maintain the content of ergothioneine in our bodies, so that the antioxidant capacity of our bodies is enhanced, and the normal growth of cells is maintained.
The tetrahydro-methyl pyrimidine carboxylic acid is also called ectoin, is from halophilic bacteria microorganism living in extreme environment, has the functions of regulating in vivo osmotic pressure, stabilizing cell membranes, enzymes and nucleic acid in the microorganism, and can survive in high-salt and extreme environment. Through research, the tetrahydro-methyl pyrimidine carboxylic acid is a cell totipotent protective agent and an anti-compression protective molecule with excellent efficacy, is a multifunctional active ingredient, has the functions of stabilizing a protein hydration layer, protecting biomacromolecules such as enzyme and DNA and a cell membrane structure, and can help cells, animals and plants to resist various adverse environments such as freezing, drought, high temperature, high salt, radiation and the like.
The tropaeolum majus extract contains benzyl thiocyanate, has antibacterial property, has antibacterial effect on staphylococcus aureus, streptococcus, escherichia coli, bacillus subtilis and part of fungi, and also has anti-inflammatory effect; can promote hair growth, and can be used for preventing alopecia and caring hair; has effects in scavenging free radicals and resisting aging. The active component of the tropaeolum majus extract also comprises arabinogalactan, which can recover the normal function of an oxygen sensor and activate the oxygenation of cells in an anoxic state so as to recover the normal metabolism function of the cells.
In the present application, the skin care composition comprises the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.1-10 parts;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 1-20 parts;
the tropaeolum majus extract is as follows: 0.5 to 15 portions;
the alfalfa extract is: 1-20 parts;
the phellodendron amurense bark extract is as follows: 0-5 parts;
the hyaluronic acid substances are: 2.5 to 20 portions.
In the present application, the skin care composition comprises the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.5 to 5 parts;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 5-15 parts;
the tropaeolum majus extract is as follows: 2.5 to 10 portions;
the alfalfa extract is: 5-15 parts;
the phellodendron amurense bark extract is as follows: 2-5 parts;
the hyaluronic acid substances are: 5 to 15 portions.
In the present application, the content of the ergothioneine matsutake extract may be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, with respect to 100 parts by weight of the total weight of the skin care composition.
In the present application, the content of the tetrahydro-methyl-pyrimidine-carboxylic acid may be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19 parts, 19.5 parts, 20 parts, relative to 100 parts by weight of the total weight of the skin care composition.
In the present application, the content of the tropaeolum extract may be 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, relative to 100 parts by weight of the total weight of the skin care composition.
In the present application, the content of the medicago sativa extract may be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19 parts, 19.5 parts, 20 parts, relative to 100 parts by weight of the total skin care composition.
In the present application, the content of the phellodendron bark extract may be 0 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 1.1 part, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts, 2.6 parts, 2.7 parts, 2.8 parts, 2.9 parts, 3 parts, 3.1 parts, 3.2 parts, 3.3 parts, 3.4 parts, 3.5 parts, 3.6 parts, 3.7 parts, 3.8 parts, 3.9 parts, 4 parts, 4.4 parts, 4.5 parts, 4.6 parts, 4.7 parts, 4.8 parts, 4.9 parts, 4.4 parts, 4.4.4 parts, 4.5 parts, 4.6 parts, 4.4.4 parts, 4.5 parts, 4.8 parts, 4.4.4 parts, 4.4 parts, 4.6 parts, 4.
In the present application, the content of the hyaluronic acid-based substance may be 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19 parts, 19.5 parts, 20 parts, relative to 100 parts by weight of the total weight of the skin care composition.
In the application, the skin care composition further comprises a humectant, wherein the humectant is one or more than two of trehalose, sodium polyglutamate and tremella polysaccharide.
The humectant may be trehalose.
The humectant may be sodium polyglutamate.
The humectant may be tremella polysaccharide.
The humectant can be trehalose and sodium polyglutamate.
The humectant can be trehalose or Tremella polysaccharide.
The humectant can be sodium polyglutamate and Tremella polysaccharide.
The humectant can be trehalose, Tremella polysaccharide, and ammonium polyglutamate.
In the present application, the humectant is contained in an amount of 0.1 to 20 parts, preferably 5 to 10 parts, based on 100 parts by weight of the total skin care composition.
In the present application, the molecular weight of the hyaluronic acid-like substance is 0.1wDa-5wDa, preferably 0.1wDa-1 wDa.
In the application, the skin care composition also comprises water and a preservative, wherein the preservative is one or more than two of phenoxyethanol, ethylhexyl glycerin, pentanediol, butanediol and methyl hydroxybenzoate.
The preservative may be phenoxyethanol.
The preservative may be ethylhexyl glycerol.
The preservative may be pentanediol.
The preservative may be methylparaben.
The antiseptic may be phenoxyethanol and ethylhexyl glycerol.
The antiseptic may be phenoxyethanol or pentanediol.
The antiseptic may be phenoxyethanol or methyl hydroxybenzoate.
The preservative can be ethylhexyl glycerol, pentanediol.
The antiseptic may be ethylhexyl glycerol, methyl hydroxybenzoate.
The preservative can be pentanediol or butanediol.
The antiseptic may be phenoxyethanol, ethylhexyl glycerol, and pentanediol.
The antiseptic may be phenoxyethanol, ethylhexyl glycerol, or butanediol.
The antiseptic may be phenoxyethanol, pentanediol, or butanediol.
The antiseptic may be ethylhexyl glycerol, pentanediol, or butanediol.
The antiseptic may be phenoxyethanol, ethylhexyl glycerol, pentanediol, or butanediol.
In the present application, the preservative is contained in an amount of 0.2 to 10 parts, preferably 0.8 to 8 parts, based on 100 parts by weight of the total skin care composition.
The application provides a preparation method of a skin care composition for resisting light pollution and light damage, which comprises the following steps:
uniformly mixing ergothioneine Armillaria matsutake extract, tetrahydro-methylpyrimidine carboxylic acid, Ecliptae herba extract and hyaluronic acid substances to obtain a mixture I, adding water into the mixture, and stirring to dissolve to obtain the skin care composition.
In this application, phellodendron amurense bark extract and alfalfa extract are added to mixture one and mixed uniformly to obtain mixture two.
In the present application, preservatives and humectants are added to the second mixture prior to the addition of water to the second mixture.
The application provides a skin care product comprising the skin care composition.
In the present application, the weight percentage of the skin care composition in the skin care product is
0.5％-20％。
In the present application, the skin care composition is present in the skin care product in an amount of 1% to 8% by weight.
In the present application, the skin care composition is present in the skin care product in an amount of 5% by weight.
The skin care composition may be present in the skin care product in an amount of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 6%, 17%, 18%, 19%, 20% by weight.
The application provides an application of the skin care composition in cosmetics, skin care products and washing products.
The cosmetic or skin care product can be cream, lotion, essence, toner, facial mask, etc.
The washing and caring product can be shampoo, hair conditioner, hair mask, etc.
Examples
The experimental methods used in the following examples are all conventional methods, unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The ergothioneine Armillaria matsutake extract, tetrahydro-methylpyrimidine carboxylic acid, hyaluronic acid, and Tremella polysaccharide are all from Huaxi biological corporation. Alfalfa extract was purchased from Shanghai Galeaf industries, Inc., cork tree bark extract was purchased from Oxue chemical, Guangzhou, Tropaeolum extract was purchased from Silab, France, trehalose was purchased from Rivergo.
Example 1
Weighing 0.1g of ergothioneine matsutake mushroom extract, 20g of tetrahydro-methylpyrimidine carboxylic acid, 0.5g of tropaeolum majus extract, 20g of alfalfa extract, 0.1g of phellodendron amurense bark extract and 2.5g of sodium hyaluronate (molecular weight is 0.1wDa), sequentially adding 6 materials into a beaker, and adding purified water until the total amount is 100 g; stirring until the materials are dissolved, and dissolving to obtain the skin care composition with light pollution and light injury resistance.
Examples 2 to 9 and comparative examples 1 to 2 were different from example 1 in the content of 6 kinds of materials, i.e., ergothioneine matsutake extract, tetrahydromethylpyrimidine carboxylic acid, tropaeolum majus extract, medicago sativa extract, phellodendron bark extract, and sodium hyaluronate, and the molecular weight of sodium hyaluronate was different.
Example 10, example 11, example 12 differ from example 4 in that example 10 has no phellodendron bark extract, example 11 has no phellodendron bark extract and no alfalfa extract, and example 12 has no alfalfa extract.
Comparative examples 3 and 4 are different from example 4 in that: comparative example 3 has no ergothioneine matsutake mushroom extract, tetrahydro methylpyrimidine carboxylic acid; comparative example 4 did not contain tropaeolum extract and sodium hyaluronate.
Comparative example 5 is an oily solution of carotene and lutein in caprylic/capric triglyceride.
The above specific parameters are shown in Table 1.
EXAMPLE 13 preparation of a serum containing a skin care composition for combating photocontamination and photodamage
5g of the skin care composition prepared in the embodiment 4, 2g of trehalose, 0.5g of tremella polysaccharide, 5g of 1, 4-butanediol and 3g of 1, 2-pentanediol are added into a beaker, then water is added until the total amount is 100g, and the water is stirred to be completely dissolved, so that the essence containing the skin care composition with the effects of resisting light pollution and light injury is obtained.
Comparative example 6
2g of trehalose, 0.5g of tremella polysaccharide, 5g of 1, 4-butanediol and 3g of 1, 2-pentanediol are added into a beaker, then water is added until the total amount is 100g, and the mixture is stirred to be completely dissolved, so that the essence of the skin care composition without light pollution and light injury is obtained.
Table 1 is a comparative table of raw material compositions of examples and comparative examples
Test examples
First, stability test
The anti-photopollution and photodamage skin care composition has strong absorption effect below 500nm, as shown in fig. 1, B in fig. 1 is a spectrum of the composition prepared in example 4 diluted by 10 times, and a is a spectrum of the composition prepared in example 4 diluted by 100 times, and it can be known from fig. 1 that the skin care composition can still efficiently filter ultraviolet and blue light even if diluted by 100 times.
To evaluate the stability of an active substance, we tested the change in absorbance value of the substance at the wavelength of maximum absorption.
The detection method comprises the following steps: before stability investigation, 0.08 percent of phenoxyethanol is added into the skin care compositions prepared in the examples 1-12 and the comparative example 5 respectively to serve as an anti-microbial agent, so that the growth of bacteria in a sample in an experimental process is prevented; the skin care composition is placed at 60 ℃/10 days and is illuminated for 10 days, samples are taken at 0 day and 10 days respectively, the change of the appearance, the pH value and the absorbance of each sample is inspected, the test result of the skin care composition of each embodiment and the comparative example at 10 days is compared with the test result at 0 day, and the test result after 10 days is greatly changed from the initial value, so that the composition is poor in stability, small in change and good in stability.
Appearance: visually observing the appearance of the sample at room temperature and under direct sunlight;
pH value test method: directly measuring skin care composition without dilution with acidimeter (Mettler acidimeter model FE 20);
the absorbance test method comprises the following steps: each composition was diluted to an appropriate concentration so that the absorbance test value was in the range of 0 to 1 (the same dilution factor of the same composition was required), and then the absorbance of the sample was measured at 422nm (example sample)/450 nm (comparative example 5 sample) using an ultraviolet-visible spectrophotometer (model: UV 1800).
The test results are shown in Table 2.
Table 2 stability test results of the respective compositions
And (3) knotting: as can be seen from the test results in Table 2, the appearance, pH and absorbance at 422nm of the skin care compositions of examples 1-12 remained substantially unchanged when the skin care compositions were left at 60 ℃ or under light for 10 days, while the appearance and absorbance of comparative example 5 were changed, indicating that the skin care compositions of the present invention having anti-photodamage and anti-pollution properties were more stable than the samples containing beta-carotene and lutein.
Second, safety test
Safety evaluations were performed by testing the cytotoxicity of the composition samples.
Sample preparation: the skin care compositions prepared in examples 1 to 4 were prepared into 10% stock solutions in serum-free DMEM (high-sugar) medium, sterilized by filtration through a 0.22 μm filter, and diluted with serum-free medium to the desired concentrations of 0.1%, 1%, and 5% when used.
Test materials and instruments: a 96-well plate, a WST-1 cell proliferation detection kit (Kayji biology), fetal bovine serum (Quacell), human epidermal keratinocyte HaCaT, and a DMEM dry powder culture medium (Gibco); inverted microscope (OLYMPUS, CKX41), clean bench (SCB-1520), carbon dioxide incubator (SANYO), digital display constant temperature water bath (Dart instruments, Tan., China), microplate rapid oscillator (Linbel), and enzyme-labeling instrument (BIO-RAD).
The test process comprises taking human epidermal keratinocyte HaCaT of logarithmic growth phase, and mixing with 2 × 104Inoculating to 96-well plate at density of 100 μ L per well, adding 10% fetal calf serum into DMEM high-sugar culture solution, placing the inoculated cells in carbon dioxide incubator at 37 deg.C and 5% CO2Culturing for 24h in a conventional way; the old culture medium was discarded, the experimental group was replaced with 100. mu.L of sample, the negative control group (control) was added with the same amount of serum-free culture medium, the blank group was cell-free, and serum-free culture medium was added alone. 4 concentration levels per sample, 6 parallel wells per level; after continuously culturing for 48h, detecting the relative proliferation rate of the cells by adopting a WST-1 method, discarding the old culture solution, adding 100 mu L of serum-free culture solution and 10 mu L of WST-1 into each hole, putting the cells into a cell culture box, continuously culturing for 2h, measuring the absorbance at the wavelength of 450nm by using an enzyme labeling instrument, wherein the relative proliferation rate (RGR) is the ratio of the absorbance of the experimental group to the absorbance of the negative control group; the results of the tests are shown in Table 3, and the samples are considered to be cytotoxic when the RGR is less than 70% according to the requirements of GB/T16886.5-2017.
Table 3 cytotoxicity tests of skin care compositions of examples 1-4
And (3) knotting: as can be seen from the results of the cytotoxicity tests of the skin care compositions prepared in examples 1 to 4 shown in table 3, the skin care compositions disclosed in the present application were safe and non-cytotoxic at concentrations of 10% or less.
Third, protection and repair testing
1. The test principle is as follows: researches show that ultraviolet and blue light irradiation with the wavelength of 280nm-500nm can cause skin cells to generate excessive active oxygen, and the excessive active oxygen can press the cells to cause cell damage and damage to the skin, so that the damage of light to the skin can be reduced by inhibiting the generation of the active oxygen or eliminating the excessive active oxygen. The experiment adopts a fluorescent probe DCFH-DA to mark intracellular ROS, and a flow cytometer is used for detecting the intracellular ROS content.
2. Light damage model: by using UVA (2000 uW/cm)2Irradiation for 60min), UVB (700. mu.W/cm)25min of irradiation) and LED blue light (415nm, 34 mW/cm)2Irradiation for 30min) to irradiate human epidermal keratinocytes HaCaT and stimulate the cells to produce Reactive Oxygen Species (ROS).
3. Test materials and instruments: 12-hole plate, fluorescent probe DCFH-DA, fetal calf serum, human epidermal keratinocyte HaCaT, ROS detection kit (Kaikyi biology), clean bench (Beijing Donghong Harer apparatus manufacturing Co., Ltd., SCB-1520), carbon dioxide incubator (SANYO), digital display constant temperature water bath (gold jar of the large instrument factory), micropore plate rapid oscillator (Linbel), flow cytometer (BD AccuriC6Plus), UV-8 type ultraviolet lamp box (Beijing electric light source institute), ST-513 type ultraviolet measuring instrument (Taiwan Chichi), LED lamp belt.
4. Sample preparation: the skin care compositions of examples 1-12 and comparative examples 1-4 were diluted 1000-fold with serum-free medium and then sterilized by filtration through a 0.22 micron filter.
Preparing a dichlorofluorescein diacetate (DCFH-DA) probe solution: diluted at 0.375. mu.L of CFH-DA to 1mL of PBS.
5. The test process of ROS protection of the sample on irradiation:
(1) cell culture, taking HaCaT cells in logarithmic growth phase, and culturing at 4 × 104The cells were inoculated in 12-well plates at a density of 2mL per well, and placed in a carbon dioxide incubator at 37 ℃ with 5% CO2Culturing for 24h under the condition;
(2) adding medicine and irradiating: discarding the old culture solution, adding the sample solution into the sample group, adding the serum-free culture solution into the negative control group, and adding the serum-free culture solution into the positive control group, wherein each hole is 2 mL; after continuously culturing for 24h, covering preservative films on the experimental group and the positive control group, then irradiating by using an ultraviolet lamp and an LED blue light lamp, and covering the negative control group by using aluminum foil paper without irradiation;
(3) and (3) detection: discarding the culture solution, washing twice with PBS, adding 1.5mL of DCFH-DA into each well, placing into a cell incubator, continuously incubating for 30min, and uniformly mixing once every 5min to fully combine the probes. Discarding the probe, washing twice with preheated serum-free medium, adding 1mL serum-free medium into each hole, and incubating at 37 ℃ for 10 min; after one PBS wash, cells were trypsinized, washed twice with PBS, resuspended at 300. mu. LPBS, and examined by flow cytometry using a dual channel, FL1-H channel, 10000 cells were collected per sample.
6. The method for repairing ROS generated by irradiation of a sample comprises the following steps:
(1) cell culture: (1) the cell culture method is the same as that of 4 (1); (2) adding medicine, irradiating and culturing: discarding 1mL of culture solution for the sample group and the positive control group, covering with a preservative film, and irradiating with an ultraviolet lamp and an LED blue light lamp. The negative control group was covered with aluminum foil paper without irradiation. After the irradiation is finished, the old culture solution is discarded, the sample group is added with the sample solution, and the model group and the negative control group are added with the serum-free culture solution, wherein each hole is 2 mL. After further incubation for 16h, all the culture medium was discarded and washed twice with PBS. (3) And (3) detection: adding 1.5mL of DCFH-DA solution into each hole, placing the mixture into a cell culture box, continuously incubating for 30min, and uniformly mixing every 5min to ensure that the probes are fully combined. The probe was discarded and washed twice with pre-warmed serum-free medium, 1mL serum-free medium was added to each well and incubated at 37 ℃ for 10 min. After one PBS wash, cells were trypsinized, washed twice with PBS, resuspended in 300. mu.L PBS, and examined by flow cytometry using a dual channel, FL1-H channel, 10000 cells were collected per sample.
7. Data processing and analysis: the ROS production rate and inhibition rate were calculated from the signal data obtained for channel 1 (FL 1-H).
ROS production (%) as the average fluorescence intensity of experiment group/average fluorescence intensity of irradiation group X100%
ROS inhibition or ROS clearance (%) -. 100% -ROS production (%)
Statistical analysis a paired t-test was performed using SPSS 19.0 software.
8. Test results the test results are shown in table 4.
TABLE 4 skin care compositions prepared in each example and comparative example before and after cell damage
Influence of active oxygen production
The test results in table 4 show that the average fluorescence intensity, i.e., the ROS content, of the positive control group is significantly higher than that of the negative control group, which indicates that the ultraviolet and blue light irradiation model can damage cells and cause the cells to generate more reactive oxygen species; examples 1 to 12, the skin care composition of the present invention with anti-light pollution and anti-light damage has the effects of inhibiting and eliminating reactive oxygen species caused by ultraviolet and blue light, and preventing damage of light pollution and anti-light damage to cells, because the ROS generation rate is significantly lower than that of the positive control group before and after the irradiation of ultraviolet and blue light; in addition, the inhibition and clearance rate of ROS in examples 1-12 are higher than that in comparative examples 1-4, which shows that the content range of the composition disclosed by the invention can effectively resist the effects of light pollution and light damage.
Fourth, cell repair test
Sample preparation: a1% stock solution was prepared from the skin care composition prepared in example 4 in serum-free medium, sterilized by filtration through a 0.22 μm filter and diluted to the desired concentration of 0.1%, 0.2% or 0.5% in serum-free medium.
Test materials and instruments: a 96-well plate, a WST-1 cell proliferation detection kit (Kaikyi organisms), fetal bovine serum, human epidermal keratinocyte HaCaT and a DMEM dry powder culture medium; an inverted microscope (OLYMPUS, CKX41), an ultra clean bench (SCB-1520, Beijing Dongherhaar instruments manufacturing Co., Ltd.), a carbon dioxide incubator (SANYO), a digital display constant temperature water bath (Takara, China, Dada instruments), a microplate fast oscillator (Linbel), a microplate reader (BIO-RAD), a UV-8 type ultraviolet lamp box (Beijing electric light research institute), a ST-513 type ultraviolet measuring instrument (Taiwan Chi), and an LED blue light strip.
The test process comprises taking human epidermal keratinocyte HaCaT of logarithmic growth phase, and mixing with 2 × 104Inoculating to 96-well plate at density of 100 μ L per well, adding 10% fetal calf serum into DMEM high-sugar culture solution, placing the inoculated cells in carbon dioxide incubator at 37 deg.C and 5% CO2Culturing for 24h in a conventional way; 1mL of culture solution is discarded from the sample group and the model group, the preservative film is covered, the ultraviolet lamp and the LED blue light lamp are used for irradiation, and the negative control group is covered by aluminum foil paper and is not used for irradiation. The old medium was discarded, the experimental group was replaced with 100. mu.L of sample, the negative control group (control) was supplemented with an equal amount of serum-free medium, and the model group was supplemented with an equal amount of serum-free medium. 3 concentration levels per sample, 6 parallel wells per level; after continuously culturing for 24h, detecting the relative proliferation rate of the cells by adopting a WST-1 method, discarding the old culture solution, adding 100 mu L of serum-free culture solution and 10 mu L of WST-1 into each hole, putting the cells into a cell culture box, continuously culturing for 2h, measuring the absorbance at the wavelength of 450nm by using an enzyme labeling instrument, wherein the relative proliferation rate (RGR) is the ratio of the absorbance of the experimental group to the absorbance of the negative control group; the results of the test are shown in Table 5.
TABLE 5 cell repair assay for skin care compositions
| Item | RGR/％ |
| Negative control group | 100％ |
| Illumination group | 75.12％ |
| The sample concentration is 0.1% | 91.56％ |
| The sample concentration is 0.2% | 92.12％ |
| The sample concentration is 0.5% | 91.52％ |
And (3) knotting: as can be seen from table 5, after the uv and blue light damage cells, the relative proliferation rate of the cells after adding the composition of the present invention is significantly higher than that of the positive control group without adding the composition of the present invention, so that the composition of the present invention has a significant cell repairing effect.
Human body evaluation test
The skin care compositions were evaluated for the effect on skin barrier, skin moisture, skin brightness under light contamination, light damage.
Subject: selecting 15 healthy volunteers aged 25-35 years and aged more than 10h by using a computer or a mobile phone every day.
Test protocol: applying the sample prepared in example 13 on one side and the sample prepared in comparative example 6 on the other side by adopting a half-face and blind test method, wherein the dosage is about 0.5g twice a day in the morning and at night, and the samples are continuously used for 4 weeks; the moisture content and the moisture loss of the skin cuticle of the left and right sides were measured before, at 1 week, 2 weeks, 3 weeks, and 4 weeks after application, respectively, and high-resolution photographs were taken to analyze changes in the facial glossiness.
Testing an instrument: skin moisture tester Corneometer CM 825(Courage + Khazaka, Germany), skin moisture loss tester Tewameter TM300(Courage + Khazaka, Germany), VISIA CR (Canfield, USA)
And (3) test results:
(1) moisture content of the stratum corneum of facial skin
The change rate of the water content of the stratum corneum was calculated according to the following formula, and the calculation results are shown in Table 6.
The formula: t (%) moisture content test value of stratum after application/moisture content test value of stratum before application × 100
TABLE 6 Effect of the serum on the moisture content of the facial stratum corneum
| Item | T0 | T7d | T14d | T21d | T28d |
| T (%) (comparative example 6) | 100 | 101.40 | 103.19 | 102.76 | 104.12 |
| T (%) (example 13) | 100 | 114.12 | 118.45 | 117.13 | 119.97 |
As can be seen from table 6, the moisture content of the stratum corneum of the face was significantly different between the serum containing the skin care composition of the present invention and the serum containing no composition, and the moisture content of the skin was about 4 to 6% higher in the serum containing the composition after week 2 than in the serum containing no composition.
(2) Percutaneous water loss of face
The rate of change of the facial percutaneous water loss was calculated according to the following formula, and the calculation results are shown in Table 7.
The formula: TEWL (%) as the amount of transdermal water dispersion after application/amount of transdermal water dispersion before application X100
TABLE 7 Effect of the essences on the amount of the transdermal Water loss
As can be seen from Table 7, the effect of the samples of example 13 and comparative example 6 on the transdermal water loss is shown that the application of the essence sample containing the composition for 28 days effectively reduces the transdermal water loss on the face and effectively improves the skin barrier function, so that the composition is further shown to have the function of repairing the skin barrier.
(3) Gloss of facial skin
The evaluation of the gloss of the facial skin was performed by taking a VISIA CR high-definition photograph and then processing the data with IPP software to obtain the gloss change of the skin in the same area, and the results are shown in table 8.
TABLE 8 results of the serum gloss test on facial skin
| Sample name | T0 | T7d | T14d | T21d | T28d |
| Skin gloss (comparative example 6) | 100 | 98.51 | 98.07 | 97.39 | 98.83 |
| Skin gloss (example 13) | 100 | 100.59 | 102.48 | 108.65 | 110.71 |
As can be seen from table 8, after 14 days of application with the essence containing the skin care composition of the present invention (example 13), the skin glossiness was significantly improved compared to the control sample (comparative example 6) not containing the skin care composition of the present invention.
In conclusion, it can be shown that the skin care essence containing the skin care composition of the present invention has the effects of repairing skin barrier and improving skin glossiness.
While embodiments of the present application have been described above in connection with specific embodiments thereof, the present application is not limited to the above-described embodiments and fields of application, which are intended to be illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto and changes may be made without departing from the scope of the invention as defined by the appended claims.
Claims (10)
1. A photostain and photodamage resistant skin care composition comprising:
an extract of Armillaria Clavipitana,
Tetrahydro-methyl-pyrimidine-carboxylic acid,
Tropaeolum majus extract and
a hyaluronic acid-like substance.
2. The skin care composition of claim 1, further comprising: alfalfa extract.
3. The skin care composition of claim 2, further comprising: cortex Phellodendri extract.
4. The skin care composition according to claim 3, comprising the following 100 parts by weight relative to the total weight of the composition:
the ergothioneine Armillaria mellea extract is: 0.1 to 10 portions;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 1-20 parts;
the tropaeolum majus extract is as follows: 0.5 to 15 portions;
the alfalfa extract is: 1-20 parts;
the phellodendron amurense bark extract is as follows: 0-5 parts;
the hyaluronic acid substances are: 2.5 to 20 portions.
5. The composition according to claim 3, characterized by comprising the following skin care composition in 100 parts by weight relative to the total weight:
the ergothioneine Armillaria mellea extract is: 0.5 to 5 parts;
the tetrahydro-methyl-pyrimidine-carboxylic acids are: 5-15 parts;
the tropaeolum majus extract is as follows: 2.5 to 10 portions;
the alfalfa extract is: 5-15 parts;
the phellodendron amurense bark extract is as follows: 2-5 parts;
the hyaluronic acid substances are: 5 to 15 portions.
6. A skin care composition according to any of claims 1 to 5 wherein the hyaluronic acid-like substance is hyaluronic acid and/or a hyaluronate salt;
the hyaluronic acid salt is selected from one or more of hyaluronic acid sodium salt, hyaluronic acid potassium salt and hyaluronic acid zinc salt, and preferably hyaluronic acid sodium salt.
7. A skin care composition according to any of claims 1 to 5 wherein the molecular weight of the hyaluronic acid-like substance is from 0.1wDa to 5wDa, preferably from 0.1wDa to 1 wDa.
8. The skin care composition according to any one of claims 1-5, further comprising a humectant selected from one or more of trehalose, sodium polyglutamate, and tremella polysaccharides.
9. The skin care composition according to any one of claims 1-5, wherein the skin care composition further comprises water and a preservative selected from one or more of phenoxyethanol, ethylhexyl glycerin, pentanediol, butanediol, and methylparaben.
10. A method of preparing a photocontamination and photodamage resistant skin care composition comprising the steps of:
uniformly mixing ergothioneine Armillaria matsutake extract, tetrahydro-methylpyrimidine carboxylic acid, Ecliptae herba extract and hyaluronic acid substances to obtain a mixture I, adding water into the mixture, and stirring to dissolve to obtain the skin care composition.
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| CN114404330A (en) * | 2021-12-27 | 2022-04-29 | 广州市第二中学 | Sunscreen composition and preparation method thereof |
| CN114533622A (en) * | 2022-01-19 | 2022-05-27 | 艾依诺科技有限公司 | Blue light prevention repairing agent and blue light prevention repairing aerosol |
| CN114533622B (en) * | 2022-01-19 | 2024-02-13 | 艾依诺科技有限公司 | Blue light prevention repairing agent and blue light prevention repairing aerosol |
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Effective date of registration: 20230627 Address after: Tianchen Avenue, Ji'nan hi tech Development Zone of Shandong Province, No. 678 250101 Patentee after: BLOOMAGE BIOTECH Co.,Ltd. Patentee after: Huaxi Biotechnology (Anhui) Co.,Ltd. Address before: Tianchen Avenue, Ji'nan hi tech Development Zone of Shandong Province, No. 678 250101 Patentee before: BLOOMAGE BIOTECH Co.,Ltd. Patentee before: SHANDONG BLOOMAGE HYINC BIOPHARM Corp.,Ltd. |