KR20100051284A - Composition for skin external application containing for improving skin wrinkle or skin whitening - Google Patents

Abstract

PURPOSE: A composition for a skin external application for improving skin wrinkle or skin whitening is provided to improve the activity of an intradermal protection factor, and to maintain the initial concentration of a natural anti-oxidation agent. CONSTITUTION: A composition for a skin external application for improving skin wrinkle or skin whitening contains silica hydride collected with anions, glacier water, oxygen dissolved water and deep water. The deep water is extracted from deep sea with the depth under 200 meters. The composition additionally contains a natural anti-oxidation agent including lipoic acid, tocopherol, coenzyme Q 10, selenium, vitamin A or vitamin C. The anion is a hydrogen ion.

Description

Composition for skin external application containing for improving skin wrinkle or skin Whitening}

The present invention relates to an external composition for skin whitening or anti-aging.

The association between aging and oxidation is well known through many studies. Especially, wrinkles and localized blackening caused by skin aging are known to be closely related to oxidation. Some people may look much younger than their age, while others may look much older than they actually are. Aging phenomena, such as skin wrinkles and localized blackening, can be caused by endogenous factors such as age increase, the shape of the skeleton and muscles, genetic factors caused by constant relaxation and contraction of muscles, environmental pollutants and stress. Can be classified as Wrinkles increase due to the reduction of keratinocytes and fibroblasts, decreased collagen synthesis, increased MMP production, and increased signal transduction of melanin due to the above-mentioned skin aging phenomena caused by various factors. Blemishes, freckles and blotch are to be increased.

In particular, recent studies have shown that, in healthy skin, free radicals are removed by bio-oxidative defense factors such as superoxide dismutase and catalase, but this removal is not complete. If not, oxidation by free radicals (a kind of free radicals) may occur, resulting in deterioration of the function of skin cells and tissues, and the cause of skin wrinkles and localized blackening. This active oxidative reactive oxygen is promoted by external (pollution, stress) and internal factors (energy metabolism in vivo) and combines with fat to make harmful substance called lipid peroxide. Lipid peroxide acts on blood vessels, causing atherosclerosis, thrombosis and other adult diseases. In addition, the decrease in collagen production ability and abnormal entanglement of collagen fibers, which are closely related to healthy skin, increases the elasticity of the skin significantly, and the glycosaminoglycan, which is a type of hyaluronic acid and glycoprotein, is responsible for skin moisture. It is reported that the production amount is remarkably lowered.

As such, various methods have been applied to improve skin aging, and as a representative method, AHA, Retinoic Acid, retinol, and vitamins are mainly applied, but they are mainly used for skin irritation and clinical efficacy. There is much debate about this. In addition, there is a limit on the amount of use and it is not a fundamental response to the oxidative action by free radicals, which is a major cause of skin aging. In addition, although many examples of using plant extracts containing antioxidant components alone, it is difficult to obtain satisfactory effects.

Meanwhile, studies on lipoic acid, tocopherol, coenzyme Q ₁₀ , carnitine, selenium, and vitamin C are mainly conducted in the food field, and in the cosmetic field, most antioxidants are used in cosmetic formulations. Easily oxidized, there is a problem that difficult to exhibit the inherent antioxidant capacity when used.

In addition, it is also important how well the effective substances, such as antioxidants contained in cosmetics, such as absorption into the skin, so efforts to improve the absorption rate of the cosmetics are continuing.

The problem to be solved by the present invention is to provide an external skin composition for the rapid absorption in the skin, effective in improving whitening and improving skin wrinkles.

In order to achieve the above object, the present invention contains an anion-captured silica hydride and / or at least one selected from the group consisting of lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin A and vitamin C, glacial water, Provided is a cosmetic composition comprising at least one of deep water and oxygen water.

The external application composition for skin according to the present invention can eliminate free radicals or increase the activity of the defense factors in the skin related to antioxidants, and further include antioxidants to enhance their antioxidant effects, as well as into the skin such as antioxidants. Increasing the absorption power provided an excellent whitening effect and skin wrinkle improvement.

Silica hydride in which the anion is used in the present invention has about 10 times more antioxidant power than the same amount of vitamin C, and has excellent effects such as skin wrinkle improvement and whitening even in cosmetics.

In order to increase the absorption rate of the silica hydride trapped with the anion into the skin, one or a mixture of deep water, glacial water, and oxygen water may be used.

The deep water is deep sea water, which is extracted from the deep sea area of about 200 m or less. Surface water acts on photosynthesis, so nitrogen, phosphorus, and nitric acid in the water are consumed, but the stratum zone does not reach sunshine, so there is no phytoplankton consuming nutrients, and photosynthesis hardly occurs. For this reason, inorganic nutrients such as nitrogen, phosphorus, and nitric acid consumed by photosynthesis remain in the depth procedure. In addition, deep water contains a lot of minerals and essential trace elements necessary for the human body, can help supplement minerals that are likely to be insufficient.

In addition, the concentration of organic matter is less than 200 m from the surface of the sea water, there is no contamination by E. coli or general bacteria, and there is little possibility of contamination by chemicals from the land or the atmosphere. Or, the temperature is low throughout the year, the change is low, has a stable low water temperature, and because it is water formed for thousands of years, its properties are stable, and it has ripening due to the action of various enzymes.

In addition, because it contains essential trace elements and various mineral components in balance, it is known to have excellent scavenging effect on active oxygen due to the action of dissolved metal ions. Effects such as activation are known.

Glacial water is formed by melting ice in high mountains or in polar regions for tens of millions of years without melting the snow, which is transformed into hard ice under pressure.

At present, glacier water is formed in the polar regions and alpine regions, which are the best clean areas on earth, and symbolizes the health and pure vitality of nature. Bilkabamba in Ecuador, Abkhazia in the Caucasus, and Hunza in the Himalayas are known as the representative glacier producing regions.

Since these glaciers are difficult to breed by microorganisms and pathogens, have a high ratio of hexagonal water, and are rich in minerals, the area where glacier water comes from is known as a longevity village.

These minerals are responsible for osmotic pressure, acid and alkali equilibrium, wound recovery and metabolic functions in the body, and play an important role in oxygen transfer and cellular respiration.

Oxygen water in the present invention refers to water containing oxygen (dissolved oxygen) dissolved in water to the supersaturated concentration is higher than the dissolved oxygen concentration dissolved in the general water. Oxygen in oxygen water is indispensable for the survival of aerobic organisms, including humans. Humans supply oxygen not only to the nose and mouth but also to the skin, and skin respiration takes up about 0.6% of the total breathing volume. Through skin respiration, very important activities such as heat dissipation of the skin, excretion of toxic substances (skin toxins), and water evaporation are performed.

Water is also deeply related to the metabolism of the skin, and as we age, water in the skin cells gradually decreases and insufficient water replenishment inhibits the turnover of the skin cells.

Therefore, in order to maintain the normal functioning of the skin and active metabolism, sufficient oxygen and water supply is necessary. The present invention is to supply the skin with oxygen and water to restore the inhibited skin metabolism, and promote the turnover of the skin by promoting the release of melanin produced in the skin has a whitening effect.

As such, deep water, glacial water, and oxygen water have excellent moisturizing and whitening effects even when used independently, but are effective on the skin. However, when used with silica hydrides with anions, they can improve the whitening and antioxidant effects. Could.

As the anion trapped in the silica hydride, hydrogen ions having the best antioxidant ability are most suitable, and their content is preferably 0.001 to 20% by weight based on the total weight of the silica hydride. At this time, when the content of the anion is less than 0.001% by weight it is difficult to expect the desired antioxidant capacity, when it exceeds 20% by weight is not properly collected. Silica hydrate supporting hydrogen anion is most suitable for the average particle size of about 200 to 500nm, but the antioxidant capacity does not vary depending on the size.

The silica hydride in which the anion is collected is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content exceeds 20% by weight, skin irritation is concerned.

In addition, the present invention further comprises one or more natural antioxidants selected from the group consisting of lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin A and vitamin C to enhance the whitening and antioxidant effects of the external preparation for skin according to the present invention. Can be.

Lipoic acid used in the present invention is a metabolic essential substance, and acts as a coenzyme (CoA) which is closely related to thiamine in the complete oxidative decarboxylation system of pyruvic acid or α-ketoglutaric acid. As a physiologically active substance such as proven efficacy, it is a material with a large application area, and cosmetics has excellent effects such as skin wrinkle improvement and whitening. The lipoic acid is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content is more than 20% by weight, skin irritation is concerned.

Tocopherol used in the present invention is one of the fat-soluble vitamins in food, four types of tocopherols (α-, β-, γ-, δ-) and four types of tocotrienols (α-, β-, γ-, δ-) Although it appears, the most common and high bioactivity is α-tocopherol, commonly referred to as vitamin E. Tocopherol's most important function in human body is antioxidant, which prevents cell membrane damage and even tissue damage by inhibiting oxidation of substances that are easily oxidized in cells, especially unsaturated fatty acids that make up cell membrane. The tocopherol is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content is more than 20% by weight, skin irritation is concerned.

Coenzyme Q ₁₀ used in the present invention participates in biochemical reactions that energize nutrients that have entered the cell together with other enzymes in the body. This substance is particularly distributed in cells with high energy demands such as cardiomyocytes and muscle cells. It also acts as a powerful antioxidant to prevent damage to cells from unstable harmful oxygen. Coenzyme Q ₁₀ is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content exceeds 20% by weight, skin irritation is concerned.

Selenium used in the present invention is a trace element (inorganic substance) essential to our body. This nutrient is an important component of antioxidant enzymes that protect cells from free radicals that occur during normal oxygen metabolism, and is essential for the normal functioning of the immune system. The selenium is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content is more than 20% by weight, skin irritation is concerned.

Vitamin A used in the present invention is one of fat-soluble vitamins, and can be synthesized artificially. It contains a lot of animal liver, egg yolks, and butter, which are poor when it causes poor development, reduced resistance to bacteria, night blindness, and keratin hardening. The yellow-red carotene found in plants such as tomatoes, carrots, and zucchini is converted to vitamin A in the animal's body. The vitamin A is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content is more than 20% by weight, skin irritation is concerned.

Vitamin C used in the present invention is necessary for tissue growth and repair because it is a basic substance for collagen formation, and is also essential for treating fractures. It strengthens the gums, improves adrenal function, and improves absorption of iron. It has an antioxidant action, which returns the oxidizing substance in the body to the reducing form. The vitamin C is contained in an amount of 0.001 to 20% by weight, preferably 0.001 to 5.0% by weight based on the total weight of the external preparation for skin. If the content is less than 0.001% by weight, the desired antioxidant capacity cannot be obtained. If the content is more than 20% by weight, skin irritation is concerned.

The external preparation composition for skin of the present invention is not particularly limited in its formulation, and may be, for example, a cosmetic composition having a formulation of a flexible cosmetic water, a nourishing cosmetics, a massage cream, a nourishing cream, a pack, a gel, or a skin adhesive cosmetic. It may be a transdermal dosage form such as lotion, ointment, gel, cream, patch or spray.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. However, the present invention is not limited only to these examples.

Test Example 1 Free Radical Scavenging Effect

1,1-diphenyl-2-picryl to determine the effect on free radical scavenging of an anion-packed silicide hydrate and a composition comprising lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin C and mixtures thereof Experiments were carried out by the (pycryl) -hydrazyl (DPPH) method, and the reagents were all purchased from Sigma (St. Louis, MO, USA). This reagent is a relatively stable free radical and is the in vitro method used primarily to confirm free radical scavenging action.

The concentrations used for the DPPH determination of lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin C and mixtures were 5.0, 1, 0.1 and 0.01% by volume, which was first placed in a 96-well plate and the total volume of DPPH prepared in 5 mM ethanol solution After 200 μl of the solution was left at 37 ° C. for 30 minutes, absorbance was observed using a 520 nm ELISA reader. Free radical scavenging action (%) was calculated by the following equation 1, the results are shown in Table 1 below. Samples used in the present invention, namely lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, and vitamins A and C were purchased from Sigma Aldrich, USA, and the anion trapped silica hydride was prepared by itself using an arc plasma.

Free radical scavenging effect (%) = 100-B / A * 100

A: Absorbance of control wells without sample

B: Absorbance of Experimental Wells Treated with Samples

Test substance 5.0% by volume 1.0 volume% 0.1 volume% 0.01 volume% Lipoic acid (A) 78.4 45.8 18.4 10.8 Tocopherol (B) 85.6 54.2 32.8 22.1 Coenzyme Q ₁₀ (C) 83.7 48.7 27.0 16.7 Selenium (D) 80.5 49.3 28.6 15.3 Vitamin A (E) 68.1 45.4 18.7 11.7 Vitamin C (F) 75.2 50.2 20.0 15.3 Anion trapping silica hydride (G) 60 30.2 10.0 5.2 G + A (1: 1) 95.7 82.1 48.5 35.1 G + B (1: 1) 92.8 80.4 45.1 25.1 G + C (1: 1) 91.5 75.3 40.5 20.6 G + D (1: 1) 92.3 85.9 50.6 28.3 G + E (1: 1) 92.9 88.7 54.3 30.4 G + F (1: 1) 94.9 89.5 56.1 31.8

As shown in the results of Table 1, lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin A, vitamin C and silica hydride trapped with anions had free radical scavenging activity, respectively, and these free radical scavenging actions were concentration dependent. It has a phosphorus effect and especially when used in combination, it can be seen that it shows a high synergistic effect.

Test Example 2 Effect of Tropoelastin and Fibrillin on Human Skin Fibroblasts

It was determined by the following method whether the antioxidant raw material increases the production of tropoelastin and fibrinine play an important role in elasticity in human skin. Fibroblasts isolated from human skin were placed in 5 × 10 ⁴ cells in each well of a 24-well cell culture plate and attached for 24 hours. After attaching the test material to the concentrations of Table 2 below, the cells were harvested after 2 days. At this time, the test material was not treated as a control. Enzyme immunoassay (ELISA) was used to quantify tropoelastin and fibrin in harvested media. The results are shown in Table 2 below, and the control group was reported as 100 and expressed as a relative ratio.

Test substance Concentration (ppm) Tropoelastin Production (%) Fibrillin production (%) Lipoic acid (A) 100 127 124 10 116 116 Tocopherol (B) 100 138 135 10 121 123 Coenzyme Q ₁₀ (C) 100 132 130 10 122 117 Selenium (D) 100 127 121 10 114 112 Vitamin A (E) 100 143 148 10 130 137 Vitamin C (F) 100 123 125 10 110 111 Anion trapping silica hydride (G) 100 105 107 10 101 103 G + A (1: 1) 100 138 136 10 125 129 G + B (1: 1) 100 149 147 10 130 131 G + C (1: 1) 100 142 140 10 132 129 G + D (1: 1) 100 138 124 10 116 115 G + E (1: 1) 100 159 159 10 142 148 G + F (1: 1) 100 135 134 10 121 123 Control 100 100 100

As can be seen in Table 2, a lot of tropoelastin and fibrillin were synthesized depending on the concentration of each test substance. In particular, all cases showed a greater synergistic effect with the use of silica hydride with anion trapped than with the natural antioxidant alone.

Test Example 3 Melanin Production Inhibition Effect

MEM containing 10% fetal bovine serum in human melanoma cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan) (Minimum Essential Medium) was incubated under 37 ℃, 5% CO ₂ conditions. The cultured cells were spread over 75 flasks so that the number of cells was 3 × 10 ⁵ per flask, and the cells were allowed to adhere to the wall for one night. After confirming that the cells adhered well, the medium contained 10 ppm of the test substance shown in Table 3 below. Changed to a new badge. At this time, a medium containing DMSO was used as a control. In this way, the cells were incubated with fresh medium containing the sample every two to three days, until the cells were filled in the flask. After the growth of the cells, the culture solution was removed, washed with PBS and dissolved with 1N sodium hydroxide to measure the absorbance at 500 nm and calculated the inhibition rate of melanin production according to Equation 2 below, the results are shown in Table 3 below.

Melanogenesis inhibition rate (%) = 100- (absorbance of each test substance / absorbance of control group × 100)

Test substance Melanin production inhibition rate (%) Lipoic acid (A) 15.2 Tocopherol (B) 12.3 Coenzyme Q10 (C) 14.7 Selenium (D) 3.45 Vitamin A (E) 11.1 Vitamin C (F) 14.8 Anion trapping silica hydride (g) 9.8 A + G, 1: 1 25.8 B + G, 1: 1 23.8 C + G, 1: 1 25.5 D + G, 1: 1 19.1 E + G, 1: 1 23.5 F + G, 1: 1 26.4

As can be seen in Table 3, it was confirmed that the melanin production inhibitory effect is excellent when the silica hydride with the anion is collected than when using the natural antioxidant alone.

[Examples 1-3 and Comparative Examples 1-11]

According to the compositions shown in Tables 4 and 6 below, the compositions of Examples 1 to 3 and Comparative Examples 1 to 11 were prepared (unit: wt%). The oil phases were all put in one reactor and warmed up to 60 ° C. to confirm the molten state, and mixed with the aqueous phase heated to 70 ° C. to prepare an emulsion. At this time, using a homogenizer (HOMOGENIZER) was emulsified at about 8,000rpm for about 5 minutes, and when the emulsification is finished, cooled to room temperature to prepare an emulsion.

division Ingredient Example 1 Example 2 Example 3 Awards Glacial water Balance - - Awards Deep water - Balance - Awards Oxygen water - - Balance Paid Lipoic acid - - - Paid Tocopherol - - - Paid Coenzyme Q ₁₀ - 1.0 - Awards Selenium - - - Paid Vitamin A - - 0.01 Awards Vitamin c 1.0 - - Awards Anion trapping silica hydride 1.0 1.0 1.0 Paid Vegetable Cured Oil 1.50 1.50 1.50 Paid Stearic acid 0.60 0.60 0.60 Paid Glycerol Stearate 1.00 1.00 1.00 Paid Ceteanyl Alcohol 2.00 2.00 2.00 Paid Arachidyl Behenyl Alcohol and Arachidylglucoside 1.00 1.00 1.00 Paid Cetylaryl Alcohol and Cetearylglucoside 2.00 2.00 2.00 Paid PEG-100 Stearate, Glycerolate and Propylene Glycol 1.50 1.50 1.50 Paid Caprylic / Carric Triglycerides 11.00 11.00 11.00 Paid Cyclomedicon 6.00 6.00 6.00 Awards Preservative, incense Quantity Quantity Quantity Awards Triethanol amine 0.10 0.10 0.10

Ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Purified water Balance Balance Balance Balance Balance Balance Balance Balance Lipoic acid - 1.00 - - - - Tocopherol - - 1.00 - - - Coenzyme Q ₁₀ - - - 1.00 - - Selenium - - - - 1.00 - Vitamin A - - - - - 0.01 Vitamin c 1.00 Anion trapping silica hydride 1.00 Vegetable Cured Oil 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol Stearate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Ceteanyl Alcohol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Arachidil Behenyl Alcohol and Arakidil Glucoside 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearylglucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate, Glycerolate and Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / Carric Triglycerides 11.00 11.00 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 Preservatives and Incense Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Triethanol amine 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10

division Ingredient Comparative Example 9 Comparative Example 10 Comparative Example 11 Awards Purified water Balance Balance Balance Paid Lipoic acid - - - Paid Tocopherol - - - Paid Coenzyme Q ₁₀ - 1.0 - Awards Selenium - - - Paid Vitamin A - - 0.01 Awards Vitamin c 1.0 - - Awards Anion trapping silica hydride 1.0 1.0 1.0 Paid Vegetable Cured Oil 1.50 1.50 1.50 Paid Stearic acid 0.60 0.60 0.60 Paid Glycerol Stearate 1.00 1.00 1.00 Paid Ceteanyl Alcohol 2.00 2.00 2.00 Paid Arachidyl Behenyl Alcohol and Arachidylglucoside 1.00 1.00 1.00 Paid Cetylaryl Alcohol and Cetearylglucoside 2.00 2.00 2.00 Paid PEG-100 Stearate, Glycerolate and Propylene Glycol 1.50 1.50 1.50 Paid Caprylic / Carric Triglycerides 11.00 11.00 11.00 Paid Cyclomedicon 6.00 6.00 6.00 Awards Preservative, incense Quantity Quantity Quantity Awards Triethanol amine 0.10 0.10 0.10

[Test Example 4] Wrinkle improvement effect in human

In order to confirm the wrinkle improvement effect of the silica hydride in which the anion was collected, the following experiment was performed to confirm the wrinkle improvement effect of Examples 1 to 3 and Comparative Examples 4, 6, and 7. 100 females in their 30s were divided into 5 groups of 20 people each to apply one of Examples 1 to 3 and Comparative Examples 4, 6 and 7 once daily for 8 weeks, and then scooped up replicas using silicone. The condition of the wrinkles was measured with a visometer (SV600, Courage + Khazaka electronic GmbH, Germany) for image analysis. The results are shown in Table 6 below, and each value is the average of each parameter value after 8 weeks of application minus the parameter value before application. In Table 7 below, R1 is the difference between the maximum and minimum values of the wrinkle contours, R-2 is a random division of the wrinkle contours by 5 spaces, and then the average of the R1 values, R3 is the highest value of the R1 values divided by 5, and R4 is the wrinkle contours. The baseline subtracts the values of each top and valley, and R5 represents the difference of the baseline of the wrinkle contour minus each wrinkle profile.

division R1 R2 R3 R4 R5 Example 1 -0.18 -0.13 -0.06 -0.05 -0.04 Example 2 -0.19 -0.16 -0.07 -0.06 -0.05 Example 3 -0.25 -0.19 -0.1 -0.09 -0.07 Comparative Example 4 0.04 0.03 0.00 -0.03 0.00 Comparative Example 6 -0.10 -0.07 -0.4 -0.03 -0.03 Comparative Example 7 0.06 0.05 0.02 -0.01 0.00

As can be seen from the results of Table 7, Examples 1 to 3 showed better skin wrinkle improvement than Comparative Examples 4, 6 and 7.

In addition, in order to confirm that the wrinkle improvement effect is increased when using glacial water, deep water and oxygen water, the following experiments were carried out to confirm the wrinkle improvement effects of Examples 1 to 3 and Comparative Examples 9 to 11.

100 females in their 30s were divided into 5 groups of 20 people each to apply one of Examples 1 to 3 and Comparative Examples 4, 6 and 7 once daily for 8 weeks, and then scooped up replicas using silicone. The condition of the wrinkles was measured with a visometer (SV600, Courage + Khazaka electronic GmbH, Germany) for image analysis. The results are shown in Table 6 below, and each value is the average of each parameter value after 8 weeks of application minus the parameter value before application. In Table 8, R1 is the difference between the maximum and minimum values of the wrinkle contours, R-2 is the wrinkle contours divided arbitrarily by 5 spaces, and then the average of the R1 values, R3 is the highest value of the R1 values divided by 5, R4 is the wrinkle contours The baseline subtracts the values of each top and valley, and R5 represents the difference of the baseline of the wrinkle contour minus each wrinkle profile.

division R1 R2 R3 R4 R5 Comparative Example 9 -0.18 -0.13 -0.06 -0.05 -0.04 Comparative Example 10 -0.19 -0.16 -0.07 -0.06 -0.05 Comparative Example 11 -0.25 -0.19 -0.1 -0.09 -0.07 Example 1 -0.21 -0.14 -0.07 -0.06 -0.07 Example 2 -0.23 -0.18 -0.08 -0.08 -0.07 Example 3 -0.27 -0.21 -0.13 -0.11 -0.09

As can be seen from the results of Table 8, Examples 1 to 3 showed a better skin wrinkle improvement than Comparative Examples 9 to 11.

Accordingly, it can be seen that the use of glacial water, deep water, oxygen water instead of purified water can increase the antioxidant effect.

[Test Example 5] Whitening effect on human skin

After attaching an opaque tape with 6 holes of 1.5cm diameter to the upper forearm of the subject, 12 healthy men were treated with 1.5-2 times the UVB of the minimum Erythema Dose. The blackening of the skin was induced by irradiating and the test materials were applied and two months later, the contrast of the skin was measured using a color difference meter. Each test substance was applied to the external preparation composition of Examples 1 to 3 and Comparative Examples 1 to 11 twice daily in the morning and evening. The determination of the effect was determined by obtaining an "L" value representing the skin's contrast as in animal testing (unburned Korean skin color generally ranges between 50 and 70). When the test substance is effective, the L value is gradually increased, and the comparison between the test substances is calculated according to the following equation (3) of the difference in skin color (ΔL) at the start and the end of the application. It is shown in Table 9 below.

ΔL * = L * value at the time of completion of coating-L * value at the start of coating

Whitening effect test on human skin Test substance Whitening effect (ΔL) Example 1 3.25 Example 2 3.76 Example 3 2.98 Comparative Example 1 1.27 Comparative Example 2 1.67 Comparative Example 3 1.82 Comparative Example 4 1.79 Comparative Example 5 1.20 Comparative Example 6 1.19 Comparative Example 7 1.20 Comparative Example 8 1.07 Comparative Example 9 2.82  Comparative Example 10 3.04 Comparative Example 11 2.08

As can be seen from the results of Table 9, Comparative Examples 9 to 11 have excellent whitening effects as compared to Comparative Examples 1 to 8, and Comparative Examples 1 to 3 using deep water, glacier water, and oxygen water are compared. Compared with Examples 9-11, the whitening effect was better.

Test Example 6 Stabilization Effect of Silica Hydride

In addition, in order to confirm the stabilization effect by the initial titer retention rate and anion trapping / silica hydride in the external preparation composition for skin, the titers of Examples 1 to 3, Comparative Examples 9 to 11 and Comparative Examples 4, 6 and 7 at 45 ° C. as follows. Were measured, and the results are shown in Table 10 below.

division Early 1 week 2 weeks 4 Weeks 8 Weeks 16 Weeks Example 1 100% 95% 88% 79% 67% 45% Example 2 100% 96% 90% 85% 82% 77% Example 3 100% 96% 90% 85% 82% 77% Comparative Example 4 100% 91% 82% 70% 40% 11% Comparative Example 6 100% 95% 88% 81% 77% 65% Comparative Example 7 100% 88% 80% 45% 32% 20% Comparative Example 9 100% 95% 88% 79% 67% 45% Comparative Example 10 100% 96% 90% 85% 82% 77% Comparative Example 11 100% 96% 90% 85% 82% 77%

As can be seen from the results of Table 8, Comparative Examples 9 to 11 was found to be significantly maintained in the initial concentration in the external preparation composition compared to Comparative Examples 4, 6 and 7. In addition, Examples 1 to 3 using oxygen water, glacier water, and deep water showed a similar effect to the stabilizing effect compared to Comparative Examples 9 to 11 using purified water. It was confirmed that there is no adverse effect on.

Claims (4)

Silica hydride with anion trapped, The present invention comprising at least one selected from glacial water, deep water, oxygen water is a skin external composition for skin whitening or anti-aging. The method according to claim 1, A skin externalizing composition for skin whitening or anti-aging, further comprising at least one natural antioxidant selected from the group consisting of lipoic acid, tocopherol, coenzyme Q ₁₀ , selenium, vitamin A and vitamin C. The external preparation for skin composition according to claim 1, wherein the anion trapped in the silica hydride is hydrogen ion. The method according to claim 1 or 2, The external preparation composition for skin is characterized in that the external radical scavenging or antioxidant.