CN110151612B - Anti-photoaging composition and application thereof in essence - Google Patents

Anti-photoaging composition and application thereof in essence Download PDF

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CN110151612B
CN110151612B CN201910588125.3A CN201910588125A CN110151612B CN 110151612 B CN110151612 B CN 110151612B CN 201910588125 A CN201910588125 A CN 201910588125A CN 110151612 B CN110151612 B CN 110151612B
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photoaging
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CN110151612A (en
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刘传伟
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Shanghai Meifute Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
    • A61K8/345Alcohols containing more than one hydroxy group
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/494Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
    • A61K8/4953Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/732Starch; Amylose; Amylopectin; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755Gymnosperms [Coniferophyta]
    • A61K8/9767Pinaceae [Pine family], e.g. pine or cedar
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/004Aftersun preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The invention relates to an anti-photoaging composition and application thereof in essence, wherein glycogen, ectoin, maritime pine bark extract, beta-glucan and glycerol are adopted as raw materials and are subjected to proper weight proportion, and multiple substances are subjected to synergistic action with each other, so that the prepared anti-photoaging composition can resist light pollution from the interior of skin, reduce free radical formation, repair DNA of damaged cells, inhibit inflammation and erythema formation, promote generation of ATP (adenosine triphosphate) of cells, hyaluronic acid, ceramide and antioxidant protein, and actively resist skin photoaging. The composition is safe, mild and non-irritant, is suitable for any skin, and according to a photoaging mechanism, aiming at each link causing skin injury, the product can safely and efficiently actively solve the photoaging problem, recover cell activity and promote collagen synthesis, so that wrinkles are reduced. The composition is simple in preparation method, is easy to add in a formula, and effectively solves the problem of photoaging.

Description

Anti-photoaging composition and application thereof in essence
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-photoaging composition and application thereof in essence.
Background
Mechanism of photoaging: the essence of ultraviolet and blue light for damaging cells is that photon energy of ultraviolet or blue light is absorbed by atoms or molecules of cells, so that the electronic energy level state of the atoms is changed, a large number of secondary electrons and free radicals are generated, and further, damage events such as cell nucleus DNA damage, collagen reduction, inflammatory factor increase and the like in the cells occur. The sun protection utilizes the principle that physical or chemical sun protection agents refract or absorb ultraviolet rays; blue light resistance is to reduce the damage to skin by absorbing blue light with a blue light absorbent. Sunscreen and anti-blue light can only initially resist damage caused by partial light, and cannot promote the skin to resist and repair photoaging.
Photoaging refers to the morphological, histological and physiological changes of skin after long-term exposure to UVA (wavelength 320-400nm), UVB (wavelength 275-320nm) and LED blue light (wavelength 460-470 nm). Skin aging caused by ultraviolet irradiation, which is often manifested by skin laxity, mottle, telangiectasia, wrinkle, and sallow skin color; the blue light radiation also causes the phenomena of skin aging acceleration, relaxation, color spots, yellow and lusterless skin, skin elasticity deficiency, wrinkle acceleration and the like.
At present, the following methods are mainly used for resisting photoaging:
the method comprises the following steps: according to the physical sun-screening principle, titanium dioxide, zinc oxide and other particles are utilized, when the sun-screening face cream is coated on the face, the sun-screening effect is achieved by reflecting sunlight like a mirror. The quality of such products depends on the quality of the treatment of the reflective particles, and in general uv light can also reach the skin through the particle gaps. The disadvantages of this type of product are: the product contains a large amount of reflective particles, is easy to block pores, is generally thick and heavy, is not suitable for sensitive muscles and pockmarked muscles, is inconvenient to remove makeup after use, is generally used only when people go out, can only resist partial ultraviolet injury, and can only passively resist light pollution and photoaging.
The method 2 comprises the following steps: by combining physicochemical UV absorbers, light pollution is resisted by reflecting and absorbing harmful UV rays, and because the chemical sunscreen molecules are absorbed by the skin, the process of absorbing UV rays occurs inside the skin and is eliminated by human metabolism. The disadvantages of this type of product are: the chemical sunscreen agent has certain irritation to skin, has high oil content in the product, is easy to cause acne formation, is not suitable for general sensitive muscles and acne muscles, is inconvenient to remove makeup after use, can be generally used only when people go out, has strong limitation, and can only passively resist light pollution and photoaging.
The method 3 comprises the following steps: blue light absorbers such as calendula extract, sophora flower bud extract, hesperidin and the like are used for preventing the blue light in sunlight and the blue light of an LED from reaching the skin to cause damage. The disadvantages of this type of product are: the LED blue light protection device is only suitable for LED blue light protection, cannot resist light damage caused by sunlight, can only passively resist LED blue light damage, and cannot actively resist light aging caused by LED blue light.
The sun protection and blue light resistance is passive light pollution resistance by reflecting or absorbing ultraviolet rays and LED blue light, the capability of protecting against the ultraviolet rays and the LED blue light is limited, and photo-aging can still be caused under the irradiation of the ultraviolet rays. Therefore, there is a need to develop an active composition that can actively resist photoaging by resisting light pollution from the inside of the skin, reducing the formation of free radicals, repairing damaged cellular DNA, inhibiting inflammation and erythema, and the like.
Disclosure of Invention
In order to solve the above problems of the prior art, the present invention provides an anti-photoaging composition and its use in essence, which can protect the skin from light pollution, reduce the formation of free radicals, repair damaged cellular DNA, inhibit inflammation and erythema, promote the production of cellular ATP, hyaluronic acid, ceramide and antioxidant proteins, and actively protect the skin from photoaging.
The technical scheme adopted by the invention is as follows:
an anti-photoaging composition comprises the following raw material components:
5-15 parts by weight of glycogen;
5-15 parts of ectoin;
2-8 parts by weight of maritime pine bark extract;
2-6 parts of beta-glucan;
8-12 parts by weight of glycerol;
44-78 parts of water.
Further preferably, the raw material components of the photoaging resistant composition comprise:
glycogen, 10 parts by weight;
10 parts of ectoin;
5 parts by weight of maritime pine bark extract;
4 parts of beta-glucan;
10 parts of glycerol;
61 parts by weight of water.
Further, adding glycerol into beta-glucan, stirring uniformly, adding water, heating and stirring, cooling, sequentially adding glycogen, ectoin and maritime pine bark extract, and stirring to obtain the photoaging resistant composition.
An anti-photoaging essence comprises the following raw material components:
79.25 to 93.9 weight portions of water;
0.5-3 parts by weight of a photoaging resistant composition;
2-4 parts of glycerol;
2-5 parts by weight of methyl propylene glycol;
0.05 to 0.1 weight portion of sodium hyaluronate;
0.05-0.15 parts of xanthan gum;
0.5 to 2.5 weight portions of squalane liposome;
1-3 parts of plant preservative.
Further preferably, the raw material components of the anti-photoaging essence comprise:
86.8 parts by weight of water;
2 parts by weight of a photoaging resistant composition;
4 parts of glycerol;
3 parts by weight of methyl propylene glycol;
0.1 part by weight of sodium hyaluronate;
0.1 part by weight of xanthan gum;
2 parts of squalane liposome;
2 parts of plant preservative. .
A method for preparing the anti-photoaging essence comprises the following steps:
(1) mixing methyl propylene glycol, glycerol, sodium hyaluronate, xanthan gum and water, and then sequentially stirring, heating, homogenizing and preserving heat to obtain a homogenized raw material;
(2) cooling the homogenized raw materials, adding squalane liposome, plant preservative and the anti-photoaging composition, uniformly stirring, cooling and subpackaging to obtain the anti-photoaging essence
Further, in the step (1), the stirring speed is 50-100r/min, and the stirring time is 5-10 min.
Further, in the step (1), the heating temperature is 70-85 ℃, and the heating time is 10-15 min.
Further, in the step (1), the homogenizing time is 70-110s, and the heat preservation time is 25-35 min.
Further, in the step (2), the temperature is reduced to 40-45 ℃, and the rest raw material components are added.
Further, in the step (2), the stirring speed is 70-90r/min, and the stirring time is 5-10 min.
Further, in the step (2), the split charging is carried out when the temperature is reduced to below 38 ℃.
Further, the application of the anti-photoaging composition in preparing an anti-photoaging product.
The effects of the main raw materials in the present invention will be briefly described below.
The glycogen is prepared by taking waxy corn starch as a raw material through an enzymatic synthesis method. Has effects in promoting production of ATP, hyaluronic acid, ceramide, and antioxidant protein; and simultaneously can resist damage caused by ultraviolet rays and inhibit erythema generated by UVB irradiation.
The ectoin is a natural protective component generated by desert halophilic bacteria in high temperature, drying, strong UV irradiation and high salinity environment, and is obtained by biological fermentation technology at present. The ectoin can effectively repair sun damage, greatly reduce formation of sun damaged cells, reduce generation of pro-inflammatory factors caused by UVA, repair cell DNA damage caused by ultraviolet rays and reduce wrinkles on the surface of skin.
The Pinus maritima bark extract is an effective component and is derived from Pinus maritima bark. The maritime pine grows in a strong wind and strong light environment, in order to resist the severe environment, a large amount of active substance procyanidin is synthesized in the maritime pine, the procyanidin is concentrated in bark, the maritime pine is protected from being invaded by strong wind and strong light, and the maritime pine has excellent antioxidant and anti-inflammatory effects, promotes cells to synthesize collagen and has an anti-aging effect.
Beta-glucan is obtained by fermenting baker's yeast, is a polysaccharide composed of glucose connected by glycosidic bonds, and has effects of keeping moisture and relieving.
The invention has the beneficial effects that:
the anti-aging skin care product is prepared by compounding 4 active substances of glycogen, ectoin, maritime pine bark extract and beta-glucan, and the synergistic effect of the substances can effectively relieve inflammation and oxidation resistance, inhibit erythema generated by UVB irradiation, repair cell DNA damage caused by ultraviolet rays and reduce wrinkles on the surface of the skin, so that the aim of resisting photoaging is fulfilled. According to the invention, the problem of photoaging is safely, efficiently and actively solved aiming at each link of skin injury events. The anti-photoaging composition of the present invention reduces free radicals caused by ultraviolet rays through the antioxidant properties of glycogen and maritime pine bark extract; the ectoin can effectively protect cell DNA from being damaged by ultraviolet rays and enhance the immune system of the skin; the maritime pine bark extract can protect the health of keratinocytes exposed to oxidative stress, restore cell viability, promote collagen synthesis, thereby reducing wrinkles; the beta-glucan, the maritime pine bark extract and the ectoin can inhibit the release of proinflammatory cytokines, so that the inflammatory reaction is inhibited; glycogen inhibits the secretion of melanin and heme by the cells after exposure to sunlight, thereby reducing the formation of erythema after exposure to sunlight.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1
The embodiment provides an anti-photoaging essence, which comprises the following raw material components:
water, 79.25 g;
anti-photoaging composition, 0.5 g;
2g of glycerol;
2g of methyl propylene glycol;
0.05g of sodium hyaluronate;
xanthan gum, 0.05 g;
squalane liposome, 0.5 g;
1g of plant preservative;
wherein the raw material components of the photoaging resistant composition comprise:
glycogen, 0.025 g;
ectoin, 0.025 g;
0.01g of maritime pine bark extract;
beta-glucan, 0.01 g;
0.04g of glycerol;
0.39g of water.
The anti-photoaging composition is prepared by the following method: adding glycerol into beta-glucan, stirring uniformly, adding water, heating to 45 ℃, stirring at 800r/min for 30min, cooling to 40 ℃, sequentially adding glycogen, ectoin and maritime pine bark extract, and stirring at 600r/min for 10min to obtain the photoaging resistant composition.
The preparation method of the anti-photoaging essence comprises the following steps:
(1) mixing all the raw materials except squalane liposome, plant antiseptic and anti-photoaging composition, stirring at 50r/min for 5min, heating at 60 deg.C for 10min, homogenizing for 70s, and keeping the temperature for 20min to obtain homogenized raw material;
(2) and cooling the homogenized raw materials to 40 ℃, adding the squalane liposome, the plant preservative and the anti-photoaging composition, stirring for 5min at a speed of 70r/min, cooling to below 38 ℃, and subpackaging to obtain the anti-photoaging essence.
Example 2
The embodiment provides an anti-photoaging essence, which comprises the following raw material components:
93.9g of water;
anti-photoaging composition, 3 g;
glycerol, 4 g;
5g of methyl propylene glycol;
0.1g of sodium hyaluronate;
xanthan gum, 0.15 g;
squalane liposome, 2.5 g;
3g of plant preservative;
wherein the raw material components of the photoaging resistant composition comprise:
glycogen, 0.45 g;
ectoin, 0.45 g;
0.24g of maritime pine bark extract;
beta-glucan, 0.18 g;
0.36g of glycerol;
water, 1.32 g.
The anti-photoaging composition is prepared by the following method: adding glycerol into beta-glucan, stirring uniformly, adding water, heating to 60 ℃, stirring at 1000r/min for 60min, cooling to 45 ℃, sequentially adding glycogen, ectoin and maritime pine bark extract, and stirring at 800r/min for 30min to obtain the photoaging resistant composition.
The preparation method of the anti-photoaging essence comprises the following steps:
(1) mixing all the raw materials except squalane liposome, plant antiseptic and anti-photoaging composition, stirring at 100r/min for 10min, heating at 70 deg.C for 15min, homogenizing for 110s, and keeping the temperature for 30min to obtain homogenized raw material;
(2) and cooling the homogenized raw materials to 45 ℃, adding the squalane liposome, the plant preservative and the anti-photoaging composition, stirring for 10min at a speed of 90r/min, cooling to below 38 ℃, and subpackaging to obtain the anti-photoaging essence.
Example 3
The embodiment provides an anti-photoaging essence, which comprises the following raw material components:
water, 86.8 g;
anti-photoaging composition, 2 g;
glycerol, 4 g;
methyl propylene glycol, 3 g;
0.1g of sodium hyaluronate;
xanthan gum, 0.1 g;
squalane liposome, 2 g;
2g of plant preservative;
wherein the raw material components of the photoaging resistant composition comprise:
glycogen, 0.2 g;
ectoin, 0.2 g;
0.1g of maritime pine bark extract;
beta-glucan, 0.08 g;
0.2g of glycerol;
1.22g of water.
The anti-photoaging composition is prepared by the following method: adding glycerol into beta-glucan, stirring uniformly, adding water, heating to 55 ℃, stirring at 900r/min for 45min, cooling to 43 ℃, sequentially adding glycogen, ectoin and maritime pine bark extract, and stirring at 700r/min for 20min to obtain the photoaging resistant composition.
The preparation method of the anti-photoaging composition comprises the following steps:
(1) mixing all the raw materials except squalane liposome, plant preservative and anti-photoaging composition, stirring at 75r/min for 7.5min, heating at 65 deg.C for 13min, homogenizing for 90s, and keeping the temperature for 25min to obtain homogenized raw material;
(2) and cooling the homogenized raw materials to 43 ℃, adding the squalane liposome, the plant preservative and the anti-photoaging composition, stirring for 8min at a speed of 80r/min, cooling to below 38 ℃, and subpackaging to obtain the anti-photoaging essence.
Comparative example
The comparative example differs from example 3 only in the raw material components of the photoaging resistant composition, the photoaging resistant composition in the comparative example does not contain glycogen and ectoin, and the other operations are the same as example 3.
Examples of the experiments
First, safety test-Patch test
Test subjects: the anti-photoaging compositions obtained in examples 1 to 3, and the anti-photoaging composition obtained in comparative example.
The test process comprises the following steps: the test population of 150 persons meeting the standard of the test subjects is randomly divided into 5 groups, and the 5 groups respectively correspond to the makeup removing products and the control group of the test subjects, and each group comprises 30 persons; putting the makeup removing products in the test objects into the small chambers of the spot testers respectively, wherein the dosage is about 0.020mL-0.025mL, and no substance is put in the control holes; applying the spot test device with the test object on the back or forearm curve side of the test object with hypoallergenic adhesive tape, and lightly pressing with palm to uniformly apply on the skin for 24 h; skin reactions were observed at 30min, 24h, and 48h after removal of the test article blotter according to the criteria shown in table 4, and the results were recorded, with the ranking criteria shown in table 1 and the test results shown in table 2.
TABLE 1 skin Patch test skin response grading Standard
Figure BDA0002115172780000101
TABLE 2 test results
Figure BDA0002115172780000102
Figure BDA0002115172780000111
As can be seen from Table 2, the anti-photoaging compositions described in examples 1-3 of the present invention and comparative example are non-irritating to the skin.
Second, efficacy test
Test subjects: the anti-photoaging compositions obtained in examples 1 to 3, and the anti-photoaging composition obtained in comparative example.
(1) Percent oxygen radical inhibition test
The photon energy of ultraviolet rays is absorbed by atoms or molecules of cells, causing the state of the electronic energy level of the atoms to change, generating a large amount of free radicals, which can hinder the normal development of the cells, interfere with the metabolism of the cells, destroy mitochondria in the cells, and the like.
The test method comprises the following steps: using Organotypical skin model
Figure BDA0002115172780000113
The skin model was pretreated with the anti-photoaging essence of the present invention, Ve and Vc were control groups, and ultraviolet irradiation (0-30mJ/cm2UVB intensity) was performed at different intensities, and the percentage of oxygen radical inhibition was measured, and the results are shown in table 3.
TABLE 3 test results
Figure BDA0002115172780000112
As can be seen from Table 3, the photoaging resistant active compositions obtained in examples 1 to 3 of the present invention are effective in reducing oxygen radicals caused by UV light; the photoaging resistant active composition obtained in the comparative example was weak in reducing oxygen radicals caused by uv light.
(2) Oxidative stress test for cuticle keratinocyte under blue light
Blue light generated by LEDs of electronic equipment such as televisions, computers, tablets, mobile phones and the like can induce oxidative stress reaction of skin cells, and more people begin to realize the importance of blue light resistance.
The test method comprises the following steps: using a blue light radiation oxidative stress model skin model, the test group was pretreated with the photoaging-resistant composition of the present invention, the control group was untreated, and different intensities of blue light irradiation (0-60J/cm2) were performed, and the medium and high dose of ROS induced apoptosis or even caused necrosis of cells by the oxidative stress reaction of cells, and the accumulation of ROS (reactive oxygen species) in epidermal keratinocytes was measured, and the results are shown in Table 4.
TABLE 4 test results
Figure BDA0002115172780000121
As can be seen from Table 4, the photoaging resistant active composition obtained in examples 1 to 3 of the present invention can effectively inhibit the generation of ROS (reactive oxygen species) in human epidermal keratinocytes under the blue light condition, thereby protecting skin cells from being damaged by the irradiation of blue light; the photoaging resistant active composition obtained in the comparative example has weak ability of inhibiting the generation of ROS (reactive oxygen species) in human epidermal keratinocytes under the condition of blue light, and cannot effectively protect skin cells from being damaged by the irradiation of blue light.
(3) Protected Langerhans cell DNA assay
Langerhans cells are antigen presenting cells of the skin, recognize, bind and process skin-invading antigens, and play an important role in contact allergy, anti-viral infection, rejection of allograft tissues and immune surveillance of epidermal cancerous cells.
The test method comprises the following steps: the inner side of the forearm (4cm2) was treated with the composition containing the anti-photoaging effect of the present invention for 2 weeks, irradiated with 1.5 times of ultraviolet biological dose (MED), and calculated by ATPase tissue staining section to obtain the langerhans cytoprotection rate, and the results are shown in table 5.
TABLE 5 test results
Figure BDA0002115172780000131
As is clear from the test results in Table 5, the photoaging-resistant compositions obtained in examples 1 to 3 of the present invention are effective in repairing DNA of damaged Langerhans cells under UV irradiation; the photoaging-resistant composition obtained in the comparative example was weaker than the compositions obtained in examples 1 to 3 in the ability to repair DNA of damaged Langerhans cells under UV irradiation.
(4) Experiment for promoting fibroblast to synthesize type I collagen
With the increase of age, the synthesis capacity of fibroblasts is reduced, if the skin lacks collagen, collagen fibers are solidified, intercellular mucopolysaccharide is reduced, the skin loses softness, elasticity and luster, and is aged, meanwhile, fiber breakage, lipoatrophy, sweat gland and sebaceous gland secretion of dermis are reduced, and a series of aging phenomena such as color spots, wrinkles and the like appear on the skin.
The test method comprises the following steps: a fibroblast skin model was established, pretreated with 3g and 6g of photoaging-resistant composition, and the content of type I collagen synthesized by fibroblasts was measured by a fluorescence labeling method, with the results shown in Table 6.
TABLE 6 test results
Figure BDA0002115172780000132
Figure BDA0002115172780000141
As can be seen from Table 6, the anti-photoaging compositions obtained in examples 1 to 3 of the present invention are effective in promoting the synthesis of type I collagen by fibroblasts; the photoaging-resistant composition obtained in the comparative example was inferior to the compositions obtained in examples 1 to 3 in the ability to promote the synthesis of type I collagen by fibroblasts.
(5) IL6, IL8 inhibition assay
Lymphokines produced by IL 6-activated T cells and fibroblasts, enable B cell precursors to become antibody-producing cells; and the colony stimulating factor can promote the growth and differentiation of original bone marrow-derived cells and enhance the lysis function of natural killer cells.
IL8 is also known as chemokine CXCL8, which is a cytokine secreted by macrophages and epithelial cells. Combines with chemokine receptors interleukin-8 receptor alpha and interleukin-8 receptor beta, has cell chemotactic effect on neutrophils, and realizes the regulation of inflammatory response.
The test method comprises the following steps: establishing an epidermal fibroblast skin model, treating each group of anti-photoaging compositions in advance, adopting bisabolol and dexamethasone as a normal phase control group, detecting the content of IL6 and IL-8 generated by fibroblasts by using sandwich ELISA (enzyme-Linked immuno sorbent assay), and detecting inflammation excited by a proinflammatory mediator IL-1 alpha, wherein the result is shown in Table 7.
TABLE 7 test results
Figure BDA0002115172780000142
As can be seen from the test results in Table 7, the anti-photoaging compositions obtained in examples 1 to 3 of the present invention can effectively inhibit the release of IL6 and IL8, thereby achieving the inhibition of the occurrence of inflammatory reaction; the photoaging-resistant composition obtained in the comparative example was weak in the ability to inhibit the release of IL6 and IL8 as compared to the compositions obtained in examples 1-3.
(6) LED blue light irradiation test
Rabbits (25) were shaved from both sides of the dorsal spine in the range of 3 x 3cm with an application area of 2.5 x 2.5 cm. 0.5ml of the anti-photoaging composition was applied to the left side of the skin, the other side of the skin was used as a control, the left side of the skin was applied 1 time a day and then irradiated with blue light for 2 hours, the operation was continued for 28 days, the remaining test substance was shaved before each application from the next day, warm water was used to remove the remaining test substance, and the number of animals with sunburn was counted after one hour observation, and the results are shown in table 8.
TABLE 8 test results
Figure BDA0002115172780000151
As can be seen from the data in Table 8, the anti-photoaging compositions obtained in examples 1 to 3 of the present invention can effectively resist the sunburn phenomenon caused by the blue light of the LED; the comparative example resulted in a much higher photoaging resistant composition than the compositions of examples 1-3 in a less effective way against sunburn caused by blue light from the LED.
Third, the trial effect of the consumer
Test subjects: the anti-photoaging essence obtained in examples 1 to 3, the anti-photoaging essence obtained in comparative example, and a commercially available anti-photoaging essence.
(1) Wrinkle area reduction test
The essence is adopted for testing: 120 test persons are tested, 24 test persons are respectively numbered 1-24, the test persons are used for 2 times in 1 day and are continuously used for 1 month, and the reduction value of the facial wrinkle area of the test persons is detected.
TABLE 9 test results
Figure BDA0002115172780000161
As can be seen from the test data in Table 9, the anti-photoaging essence obtained in the examples 1 to 3 of the invention can effectively reduce skin wrinkles, and the effect is superior to that of the comparative example and the commercially available anti-photoaging essence.
(2) Erythema index reduction test
The essence is adopted for testing: 125 tested persons are respectively numbered 1-25 in each group of 25 persons, the tested persons are subjected to skin erythema index test, the corresponding essence is smeared on the left arm of each person for 1 day for 2 times, the right arm of each person is used as a blank control for 2 months continuously, and the skin erythema index is observed and recorded.
TABLE 10 test results
Figure BDA0002115172780000171
Figure BDA0002115172780000181
As can be seen from the test data in Table 10, the anti-photoaging essence obtained in the examples 1 to 3 of the present invention can effectively reduce skin erythema, and the effect is superior to that of the comparative example and the commercially available anti-photoaging essence.
In conclusion, the photoaging resistant active composition can effectively inhibit free radicals caused by ultraviolet rays and LED blue light, repair damaged nuclear DNA in cells, promote collagen synthesis, inhibit inflammatory reaction, reduce erythema after sunburn and the like, and can effectively resist photoaging.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (10)

1. The photoaging-resistant composition is characterized by comprising the following raw materials:
5-15 parts by weight of glycogen;
5-15 parts of ectoin;
2-8 parts by weight of maritime pine bark extract;
2-6 parts of beta-glucan;
8-12 parts by weight of glycerol;
44-78 parts of water.
2. The photoaging-resistant composition according to claim 1, wherein the raw materials consist of:
glycogen, 10 parts by weight;
10 parts of ectoin;
5 parts by weight of maritime pine bark extract;
4 parts of beta-glucan;
10 parts of glycerol;
61 parts by weight of water.
3. A process for the preparation of a photoaging-resistant composition according to claim 1 or 2, comprising the steps of:
adding glycerol into beta-glucan, stirring uniformly, adding water, heating and stirring, cooling, sequentially adding glycogen, ectoin and a maritime pine bark extract, and stirring to obtain the photoaging resisting composition.
4. The anti-photoaging essence is characterized by comprising the following raw materials:
79.25 to 93.9 weight portions of water;
the photoaging resistant composition of claim 1 or 2, 0.5 to 3 parts by weight;
2-4 parts of glycerol;
2-5 parts by weight of methyl propylene glycol;
0.05 to 0.1 weight portion of sodium hyaluronate;
0.05-0.15 parts of xanthan gum;
0.5 to 2.5 weight portions of squalane liposome;
1-3 parts of plant preservative.
5. The anti-photoaging essence of claim 4, wherein the raw materials comprise:
86.8 parts by weight of water;
the photoaging resistant composition of claim 1 or 2, 2 parts by weight;
4 parts by weight of glycerol;
3 parts by weight of methyl propylene glycol;
0.1 part by weight of sodium hyaluronate;
0.1 part by weight of xanthan gum;
2 parts of squalane liposome;
2 parts of plant preservative.
6. The method for preparing the anti-photoaging essence of claim 4 or 5, comprising the steps of:
(1) mixing methyl propylene glycol, glycerol, sodium hyaluronate, xanthan gum and water, and then sequentially stirring, heating, homogenizing and preserving heat to obtain a homogenized raw material;
(2) and cooling the homogenized raw materials, adding squalane liposome, a plant preservative and the anti-photoaging composition, uniformly stirring, cooling and subpackaging to obtain the anti-photoaging essence.
7. The method for preparing the anti-photoaging essence of claim 6, wherein in the step (1), the stirring speed is 50-100r/min, and the stirring time is 5-10 min;
the heating temperature is 70-85 ℃, and the heating time is 10-15 min;
the homogenizing time is 70-110s, and the heat preservation time is 25-35 min.
8. The method for preparing the anti-photoaging essence of claim 6, wherein in the step (2), the temperature is reduced to 40-45 ℃, the rest raw material components are added, the stirring speed is 70-90r/min, and the stirring time is 5-10 min.
9. The method for preparing the anti-photoaging essence of claim 6, wherein in the step (2), the anti-photoaging essence is packaged when the temperature is reduced to below 38 ℃.
10. Use of a photoaging-resistant composition as claimed in claim 1 or 2 for the preparation of a photoaging-resistant product.
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