CN109549893B - Composition and essence milk with blue light pollution resistance and repair effects and preparation method of essence milk - Google Patents

Composition and essence milk with blue light pollution resistance and repair effects and preparation method of essence milk Download PDF

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CN109549893B
CN109549893B CN201910058665.0A CN201910058665A CN109549893B CN 109549893 B CN109549893 B CN 109549893B CN 201910058665 A CN201910058665 A CN 201910058665A CN 109549893 B CN109549893 B CN 109549893B
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blue light
essence milk
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CN109549893A (en
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谢水林
赵美贺
朱军
孟丽
魏坤
王晓慧
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Guangzhou Rui Sen Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706Algae
    • A61K8/9722Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/004Aftersun preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18

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Abstract

The invention relates to a composition and essence milk with blue light pollution resistance and repair effects, and also relates to a preparation method of the essence milk, belonging to the technical field of cosmetics. The essence milk provided by the invention can resist blue light pollution and has a repairing effect; the functional components of the essence milk comprise a chlorella extract, a crambe extract, a ginger flower root extract and a vernonia ulmaria extract, wherein the weight ratio of the chlorella extract to the crambe extract to the ginger flower root extract to the vernonia ulmaria extract is 1-10: 2-15: 1-10, the functional components can resist pollution and damage of blue light in all aspects, and can repair skin inflammation and damage caused by the blue light, so that the survival capability and the defense capability of skin cells are provided, and the aging of the skin cells is delayed.

Description

Composition and essence milk with blue light pollution resistance and repair effects and preparation method of essence milk
Technical Field
The invention relates to a composition and essence milk with blue light pollution resistance and repair effects, and also relates to a preparation method of the essence milk, belonging to the technical field of cosmetics.
Background
The 'blue light' is commonly called 'high-energy short-wave blue light', and is light with a wavelength of 400-480 nm and relatively high energy. Blue light pollution refers to exposure to artificial blue light of "hyperlinked lifestyle", which is mainly from computers, projectors, movie screens, LED displays, mobile phones, laser pens, fluorescent lamps, outdoor light boxes, LED lights and bathroom heaters. Blue light not only affects the secretion of melatonin, but also has a significant inhibitory effect on melatonin, which is a hormone assisting sleep. People not only disturb the normal biological clock of the human body when contacting blue light for a long time or before sleeping, but also cause a series of health problems such as insomnia and the like caused by the reduction of melatonin in the human body. In daily life, people are exposed to blue light more and more frequently, so that skin is excessively exposed to the blue light, and the self-barrier repair capacity of the skin is reduced, and aging is accelerated. For example, exposure of the skin to visible light or high-energy visible light KEV causes skin cells to exert a bad set of effects: increased reactive oxygen species, mitochondrial DNA damage, delayed epidermal barrier repair, increased inflammatory cascade, carotenoid depletion in the dermis, langerhans cell immunosuppression. Therefore, just as well as protection against UV or air pollution, it is also necessary to protect the skin against blue light pollution, which helps to slow down premature aging and damage to the skin.
The chlorella extract is obtained from chlorella rich in various nutrients such as protein, lipid, carbohydrate, vitamins, minerals, etc. Chlorella is belonging to Chlorella, Chlorophyceae, and contains biologically active substances with effect of accelerating growth of bacteria, animals and plants. The chlorella extract mainly contains chlorella growth factor which contains abundant nucleic acid and nucleoprotein, has the functions of repairing human body cells, resisting aging and activating immunocytes, has strong binding force with toxins and can expel the toxins out of a body. Therefore, the chlorella growth factor has good effect on the skin.
Crambe extract is derived from the robust saline-soil plant crambe which is living in special ecological environments. Contains various vitamins, phytosterol, sulfat and carotenoid, and has effects of stimulating renewal of skin basal layer cells, protecting stem cells from damage of UVB, stimulating synthesis of ceramide II, and reducing degradation of collagen and GAC caused by UVA, thereby having antiaging, free radical scavenging, and photoaging relieving effects.
The Zingiber officinale root extract is a unique extract from the root of Iris sieboldii Madagasca, is rich in saccharides, has anti-blue light and anti-UVA effects by regulating autophagy, and has anti-oxidation, anti-pollution and cell detoxification effects by protecting the mitochondrial network and energy metabolism of UVA changes.
The Ulmus pumila extract is extracted from root bark of Ulmus pumila, and is rich in natural polysaccharides such as galactose, rhamnose, glucose, glucosamine, and trehalose. The Ulmus pumila extract can inhibit hyaluronidase activity and reduce transdermal water loss (TEWL), and is suitable for strong moisturizing and antiaging formulation. Meanwhile, the composition can inhibit the release of IL-8, IL-6 and PEG2 (prostaglandin), has good anti-inflammatory effect, and can repair photodamage caused by UVA irradiation and improve the survival capability of cells.
With the pursuit of beauty, the medical and beauty industry is developed vigorously, and more people who love beauty receive medical beauty treatment. However, the skin after medical beauty is very fragile, and the people cannot avoid the invasion of blue light. Therefore, a skin care product which can resist blue light and has a repairing effect is urgently needed, the types of cosmetics in the market are various, most of essence products mainly have whitening, wrinkle resistance, oxidation resistance and the like, and the skin care product not only has a single function effect, but also has a poor effect. The invention provides the essence milk with the main functional components including the chlorella extract, the crambe extract, the ginger flower root extract and the elm extract, which not only can resist blue light pollution, but also has the function of repairing efficacy, and is beneficial to improving the adverse effect of blue light on the skin of people.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a composition which can resist blue light pollution and has a repairing effect.
The invention also aims to provide the essence milk which can resist blue light pollution and has a repairing effect.
The invention further aims to provide a preparation method of the essence milk.
In order to achieve the purpose, the invention adopts the technical scheme that: a composition with blue light pollution resisting and repairing effects comprises Chlorella extract, crambe extract, Zingiber officinale Roscoe root extract, and Ulmus pumila extract. In the prior art, a single extract is generally adopted for blue light pollution resistance or blue light pollution repair, however, the single extract has a single effect and a poor effect, and the double effects of blue light pollution resistance and blue light pollution repair cannot be achieved at the same time.
Preferably, the weight ratio of the chlorella extract to the crambe extract to the ginger flower root extract to the elm extract is 1-10: 2-15: 1-10.
Preferably, the weight ratio of the chlorella extract to the crambe extract to the ginger flower root extract to the elm extract is 1-6: 3-9: 1.5-7: 1-6.
Preferably, the weight ratio of the chlorella extract, the crambe extract, the ginger flower root extract and the elm extract is 5:8:6: 5. The chlorella extract, the crambe extract, the ginger flower root extract and the elm extract are compounded according to the proportion, so that the blue light pollution resistance and the repairing effect are the best.
The invention provides an essence milk with blue light pollution resistance and repair effects, which comprises an A-phase substance, a B-phase substance, a C-phase substance and water; wherein, the dosage of each component in the phase A substance comprises the following components in percentage by weight: 1-8% of glycerol, 1-5% of butanediol, 1-5% of 1, 3-propylene glycol, 0.01-1.5% of small molecular sodium hyaluronate, 0.1-1% of xanthan gum, 0.2-0.5% of glycerol stearate, 0.01-0.5% of hydrogenated lecithin, 0.1-3% of sorbitol and 0.1-1% of phenoxyethanol; the dosage of each component in the phase B material comprises: 0.1-1% of purslane extract, 1-10% of chlorella extract, 2-15% of crambe extract, 1-15% of ginger flower root extract and 1-10% of elm extract; the dosage of each component in the phase C material comprises: 0.01-0.5% of panthenol, and the balance of water to 100%.
Preferably, the essence milk provided by the invention comprises the following components in the dosage of the phase A material: 1-8% of glycerol, 1-5% of butanediol, 1-5% of 1, 3-propylene glycol, 0.01-1.5% of small molecular sodium hyaluronate, 0.1-1% of xanthan gum, 0.2-0.5% of glycerol stearate, 0.01-0.5% of hydrogenated lecithin, 0.1-3% of sorbitol and 0.1-1% of phenoxyethanol; the dosage of each component in the phase B material comprises: 0.1-1% of purslane extract, 1-6% of chlorella extract, 3-9% of crambe extract, 1.5-7% of ginger flower root extract and 1-6% of elm extract; the dosage of each component in the phase C material comprises: 0.01-0.5% of panthenol.
Most preferably, the essence milk provided by the invention comprises the following components in the dosage of the phase A material: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol; the dosage of each component in the phase B material comprises: 0.5% of purslane extract, 5% of chlorella extract, 8% of crambe extract, 6% of ginger flower root extract and 5% of elm extract; the dosage of each component in the phase C material comprises: panthenol 0.3%.
The percentage is the weight percentage.
The invention provides a preparation method of essence milk with blue light pollution resistance and repair effects, which comprises the following steps:
(1) respectively adding water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Compared with the prior art, the invention has the beneficial effects that:
(1) the essence milk provided by the invention can resist blue light pollution and has a repairing effect; the functional components of the essence milk comprise chlorella extract, crambe extract, ginger flower root extract and elm extract, and the functional components can resist the pollution and damage of blue light in all aspects and repair skin inflammation and damage caused by blue light, so that the survival capability and the defense capability of skin cells are provided, and the aging of the skin cells is delayed;
(2) according to the composition provided by the invention, the chlorella extract, the crambe extract, the ginger flower root extract and the ulmus pumila extract are reasonably compounded in a certain range, the synergistic effect is obvious, and the blue light pollution resistance and repair effect of the composition are obviously better than the compounding effect of three components;
(3) the functional components of the essence milk provided by the invention are plant extract compositions, and the essence milk is natural, safe and free of stimulation;
(4) the essence milk provided by the invention is simple in preparation method, stable in process, worthy of popularization and application, and suitable for industrial batch production.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
The raw materials or auxiliary materials used in the essence milk with the blue light pollution resistance and the repair effect are purchased from the market.
Example 1
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 1% of glycerol, 5% of butanediol, 1, 3-propylene glycol, 1.5% of small molecule sodium hyaluronate, 1% of xanthan gum, 0.2% of glycerol stearate, 0.01% of hydrogenated lecithin, 0.1% of sorbitol and 1% of phenoxyethanol;
phase B: 0.1% of purslane extract, 1% of chlorella extract, 3% of crambe extract, 1.5% of ginger flower root extract and 1% of elm extract;
and C phase: 0.5 percent of panthenol;
water: 82.09 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 82.09% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Example 2
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 2% of chlorella extract, 6% of crambe extract, 3% of ginger flower root extract and 2% of elm extract;
and C phase: 0.3 percent of panthenol;
water: 72.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 72.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Example 3
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 5% of chlorella extract, 8% of crambe extract, 6% of ginger flower root extract and 5% of elm extract;
and C phase: 0.3 percent of panthenol;
water: 61.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 61.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Example 4
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 6% of chlorella extract, 9% of crambe extract, 7% of ginger flower root extract and 6% of elm extract;
and C phase: 0.3 percent of panthenol;
water: 57.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 57.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Example 5
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 8% of glycerol, 1% of butanediol, 5% of 1, 3-propylene glycol, 0.01% of small molecule sodium hyaluronate, 0.1% of xanthan gum, 0.5% of glycerol stearate, 0.5% of hydrogenated lecithin, 3% of sorbitol and 0.1% of phenoxyethanol;
phase B: 1% of purslane extract, 10% of chlorella extract, 15% of crambe extract, 15% of ginger flower root extract and 10% of ulmus pumila extract;
and C phase: 0.01 percent of panthenol;
water: 30.78 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 30.78% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Comparative example 1
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 5% of chlorella extract, 8% of crambe extract and 6% of ginger flower root extract;
and C phase: 0.3 percent of panthenol;
water: 66.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 66.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Comparative example 2
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 5% of chlorella extract, 8% of crambe extract and 5% of ulmus pumila extract;
and C phase: 0.3 percent of panthenol;
water: 67.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 67.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Comparative example 3
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 5% of chlorella extract, 6% of ginger flower root extract and 5% of ulmus pumila extract;
and C phase: 0.3 percent of panthenol;
water: 69.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 69.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Comparative example 4
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract, 8% of crambe extract, 6% of ginger flower root extract and 5% of elm extract;
and C phase: 0.3 percent of panthenol;
water: 66.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 66.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Comparative example 5
An essence milk with blue light pollution resistance and repair effects comprises the following components in percentage by weight:
phase A: 5% of glycerol, 2% of butanediol, 3% of 1, 3-propylene glycol, 1% of small molecular sodium hyaluronate, 0.5% of xanthan gum, 0.3% of glycerol stearate, 0.3% of hydrogenated lecithin, 1% of sorbitol and 0.5% of phenoxyethanol;
phase B: 0.5% of purslane extract;
and C phase: 0.3 percent of panthenol;
water: 85.6 percent;
the preparation method of the essence milk comprises the following steps: (1) respectively adding 85.6% of water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
Product evaluation
Firstly, evaluating the safety of products
The blue light-resistant repair essence milks of examples 1 to 5 and comparative examples 1 to 5 were evaluated for the irritation of cosmetics.
The tested population is female, 90 people and between 16 and 65 years of age. The skin of the subject is healthy, has no allergic history of the skin disease, and meets the voluntary entry standard of the subject. Randomly grouped into 5 groups of 18 people each.
The test method is a human body patch test. Selecting a qualified spot tester, placing 0.020-0.025g of a tested object in the spot tester, sticking the spot tester with the tested object on the back or the bent side of the forearm of the tested object by using a non-irritating adhesive tape, and lightly pressing with a palm to uniformly stick the spot tester on the skin for 24 hours. And (5) removing the tested spot tester for 30min, and observing skin reaction after the indentation disappears. If the result is negative, the test is observed once more at 24h and 48h after the patch test. The results were recorded according to the reference standards of "standards for hygiene of cosmetics" in 2007 and "standards for safety of cosmetics" in 2015.
Evaluation criteria: level 0: negative reaction; level 1: suspicious reactions, only faint erythema; and 2, stage: weak positive reaction, erythema, infiltration, edema, and possibly pimple; and 3, level: strong positive reaction, erythema, infiltration, edema, pimple and reaction beyond the tested area; 4, level: very strong positive reaction, obvious erythema, severe infiltration, edema, herpes fusiformis and reaction beyond the tested area.
The results of the patch test on the skin of a human body show that: the skin reactions of all the test subjects are observed 24h and 48h after the test by the patch test of the essence milk of the examples 1-5 and the comparative examples 1-5, and the skin reactions of all the test subjects are negative, so that the blue light resistant repair essence milk prepared by the invention is safe to use.
Second, product functionality evaluation
1. Anti-blue light performance detection
Blue light resistance performance detection is carried out on the blue light resistance repair essence emulsions of examples 1-5 and comparative examples 1-5
The essence milks of examples 1 to 5 and comparative examples 1 to 5 were dissolved in dimethyl sulfoxide, respectively, to form corresponding sample solutions. A certain amount of Human Epidermal Keratinocytes (HEK) and Human Dermal Fibroblasts (HDF) were cultured in 96-microwell plates, the number of cells in each microwell plate being the same. The 96-well plates of cultured cells were covered with flat-bottom sterilized plates containing the sample solutions of examples 1-5 and comparative examples 1-5, and LED (476nm, 1900lux) blue light irradiation was performed for 9 hours above the sterilized plates at a distance of 30cm, followed by cell viability assay with CellTiter96(MTS), each treatment being repeated three times.
The detection method comprises the following steps: the degree of damage of blue light to Human Epidermal Keratinocytes (HEK) and Human Dermal Fibroblasts (HDF) under the serum protective conditions of examples 1 to 5 and comparative examples 1 to 5 was investigated by measuring the survival rate and proliferation rate of metabolically active cells using CellTiter96(MTS), and the results are shown in table 1.
Blank control: cells were not exposed to blue light at room temperature (37 ℃).
Table 1 blue light resistance test of each group of essence milks
Sample (I) Human epidermal keratinocyte activity (%) Human dermal fibroblast Activity (%)
Example 1 86 88
Example 2 92 93
Example 3 98 99
Example 4 98 99
Example 5 98 98
Comparative example 1 62 59
Comparative example 2 58 57
Comparative example 3 59 58
Comparative example 4 51 54
Comparative example 5 23 21
Blank control 100 100
From the results in table 1, after the LED (476nm, 1900lux) blue light is irradiated for 9 hours, the activity of human epidermal keratinocytes and the activity of human dermal fibroblasts of examples 1 to 5 are both higher than those of comparative examples 1 to 5, which indicates that the capability of the essence milk of examples 1 to 5 for resisting blue light is higher than that of comparative examples 1 to 5, and further indicates that the efficacy composition added in the essence milk of examples 1 to 5 has stronger blue light filtering capability and obvious synergistic effect, and can block the damage of blue light to the skin, therefore, the essence milk formula of examples 1 to 5 is better than that of comparative examples 1 to 5; wherein, the human epidermal keratinocyte activity and the human dermal fibroblast activity of the examples 4 and 3 are higher than those of the examples 1, 2 and 5, which shows that the weight ratio of the chlorella extract, the crambe extract, the ginger flower root extract and the ulmus pumila extract is optimal in the efficacy composition of the examples 3 and 4.
2. Inhibiting cellular reactive oxygen species production
Cell-inhibiting active oxygen production test was performed on the blue-light-resistant repair essence milks of examples 1 to 5 and comparative examples 1 to 5
The essence milks of examples 1 to 5 and comparative examples 1 to 5 were dissolved in dimethyl sulfoxide, respectively, to form corresponding sample solutions. 100 healthy mice with the weight of 20-30g are selected and randomly divided into 10 groups, each group comprises 10 mice, and 5 test groups and 5 control groups are arranged. The test samples of examples 1-5 and comparative examples 1-5 were applied to the exposed skin of the above 10 groups of mice one by one under the condition of 425nm of LED blue light (3mW/cm2 for 18min), wherein, the mice of the groups of the test groups of examples 1-5 and the mice of the groups of the control groups of comparative examples 1-5 were applied at a dose of 1ml/cm, and after 24h, the results were quantitatively analyzed by CellROX fluorescence intensity staining and Volocity image software, the higher the value of the cellular active oxygen indicates the poorer the blue light resistance of the test sample, and the cellular active oxygen changes of the mice were observed at 7 th, 14 th, 21 th and 30 th days after the treatment within 1 month, respectively, and the results are shown in Table 2.
TABLE 2 Effect of each group of essence milks on mouse skin cell reactive oxygen species
Figure BDA0001953410380000151
The results in table 2 show that the levels of reactive oxygen species in skin cells of mice are equivalent before the application of the essence milks of examples 1 to 5 and comparative examples 1 to 5, that is, on day 0, but after the application of each group of essence milks, the corresponding levels of reactive oxygen species in skin cells of mice are significantly lower than those of comparative examples 1 to 5, which indicates that the blue light pollution resistance repair essence milks of examples 1 to 5 can effectively inhibit the increase of the levels of reactive oxygen species in skin cells of mice under blue light pressure, and the effect is better than that of 1 to 5 groups with a large proportion, wherein the inhibition effect of example 3 is the best, so as to further illustrate that the blue light pollution resistance repair essence milks of examples 1 to 5 of the present invention have reasonable and obvious synergy components and have better blue light resistance.
3. Evaluation of repair Effect
Evaluation of repair Effect on blue light-resistant repair essence milks of examples 1 to 5 and comparative examples 1 to 5
The repairing effect evaluation test is carried out on 60 volunteers, namely 30 men and 30 women, which are 25-45 years old and have different degrees of damage to facial skin (such as dry skin, desquamation, pruritus and the like). The test time was 28 days, and each group was divided into 3 test groups and 3 control groups. During the test, each volunteer of 10 test groups was applied with the essence milks prepared in examples 1 to 5 and comparative examples 1 to 5, respectively, once a day in the morning and at the evening after washing the face, and each volunteer of the control group was not applied with any other cosmetics. The test and control groups were normally exposed to the LED blue light environment for 5 hours per day and evaluated 28 days after testing.
Evaluation criteria: more than 80% of the skin damage repairs were effective, and the evaluation results are shown in table 3.
TABLE 3 evaluation of the repair Effect of the essential milks of each group
Figure BDA0001953410380000161
The test results in table 3 show that the effective rate of skin repair of each volunteer in the test group is higher than that of the control group, and the skin damage repair of examples 2 to 5 is more than 80%, wherein the effective rate of skin repair of examples 3 to 5 reaches 90%, and the skin damage repair of comparative examples 1 to 5 is less than 80%, which indicates that the blue light resistant repair essence emulsion of the present invention has a good repair effect, and the repair effect is the best with the formulas of example 3, example 4 and example 5.
In conclusion, the essence milk provided by the invention can resist blue light pollution, has a repairing effect, integrates product functionality evaluation, and takes the formula of the embodiment 3 as the optimal essence milk formula of the invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (5)

1. A composition having blue light pollution resistance and blue light restoration effects, wherein the composition comprises a purslane extract, a chlorella extract, a crambe extract, a ginger root extract and a vernonia extract; the weight ratio of each component in the composition is as follows: and (3) purslane extract: the chlorella extract: and (3) sea cabbage extract: ginger flower root extract: the ulmus pumila extract is 0.1-1: 1-10: 2-15: 1-10.
2. An essence milk with blue light pollution resistance and repair effects is characterized by comprising an A phase substance, a B phase substance, a C phase substance and water;
wherein the content of the essence milk in percentage by weight is as follows:
the phase A material comprises: 1-8% of glycerol, 1-5% of butanediol, 1-5% of 1, 3-propylene glycol, 0.01-1.5% of small molecular sodium hyaluronate, 0.1-1% of xanthan gum, 0.2-0.5% of glycerol stearate, 0.01-0.5% of hydrogenated lecithin, 0.1-3% of sorbitol and 0.1-1% of phenoxyethanol;
the phase B material comprises: 0.1-1% of purslane extract, 1-10% of chlorella extract, 2-15% of crambe extract, 1-15% of ginger flower root extract and 1-10% of elm extract;
the phase C material comprises: 0.01 to 0.5 percent of panthenol;
the water is complemented to 100%.
3. The essence milk with blue light pollution resistance and repair efficacy according to claim 2, wherein the phase B material comprises: 0.1-1% of purslane extract, 1-6% of chlorella extract, 3-9% of crambe extract, 1.5-7% of ginger flower root extract and 1-6% of elm extract.
4. The essence milk with blue light pollution resistance and repair efficacy according to claim 2, wherein the phase B material comprises: 0.5% of purslane extract, 5% of chlorella extract, 8% of crambe extract, 6% of ginger flower root extract and 5% of elm extract.
5. The method for preparing the essence milk with the blue light pollution resistance and the repair efficacy according to any one of claims 2 to 4, comprising the following steps:
(1) respectively adding water and the phase A substance into an emulsifying pot, mixing, heating to 75-85 ℃, uniformly stirring and mixing at the stirring speed of 1200-1500r/min, and then sterilizing for 15-20min to obtain a mixed solution a;
(2) when the temperature of the mixed solution a is reduced to below 45 ℃, adding the phase B substance, and stirring for 30min at the stirring speed of 1200-1500r/min to obtain a mixed solution B;
(3) and when the mixed solution b is cooled to the room temperature, adding the phase C substance, and stirring at the stirring speed of 1200-1500r/min for 30-60min to obtain the essence milk.
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