CN114073658B

CN114073658B - Essence composition containing liposome capable of resisting photoaging injury and preparation method thereof

Info

Publication number: CN114073658B
Application number: CN202111397908.7A
Authority: CN
Inventors: 陈茹; 杨帆; 周紫燕; 李沫; 郭苗; 周正
Original assignee: Hubei Maishite Biotechnology Co Ltd
Current assignee: Hubei Maishite Biotechnology Co Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2023-09-15
Anticipated expiration: 2041-11-23
Also published as: CN114073658A

Abstract

The invention discloses an essence composition containing liposome capable of resisting photoaging injury and a preparation method thereof. The essence composition comprises the following raw material components: 0.1-20 parts of liposome capable of resisting photoaging injury and 80-99.9 parts of cosmetic auxiliary material matrix. Wherein the main components of the liposome capable of resisting photoaging injury comprise: 0.1-5 parts of a calli extract of alpine leontopodium herb, 0.1-10 parts of a lysate of a fermentation product of two-split yeasts, 0.1-3 parts of an spartina crispa extract and 0.01-1 part of carnosine. The composition protects cellular DNA and dermis layer structure by improving free radical scavenging efficiency, and has synergistic effect on resisting damage caused by photoaging; the composition is added into skin care products, can effectively protect skin from being damaged by ultraviolet rays, avoid degradation of hyaluronic acid, collagen and elastin in dermis layers, improve cell activity and reduce skin redness symptoms.

Description

Essence composition containing liposome capable of resisting photoaging injury and preparation method thereof

Technical Field

The invention relates to a skin care product and a preparation method thereof, belongs to the technical field of daily chemicals, and in particular relates to an essence composition containing liposome capable of resisting photoaging injury and a preparation method thereof.

Background

Skin aging includes intrinsic physiological aging and extrinsic ultraviolet-based environmental factor-induced aging. Among them, skin aging caused by ultraviolet rays is called photoaging. Premature skin aging caused by ultraviolet rays, including sunburn, wrinkles, surface dryness, rough peeling, photo carcinogenesis, and the like, can be reduced or even eliminated by reducing contact with ultraviolet rays or counteracting the deleterious effects of ultraviolet rays.

Ultraviolet rays are divided into three wave bands, namely long-wave UVA (320-400 nm), medium-wave UVB (280-320 nm), short-wave UVC (200-280 nm), and the shorter the wavelength is, the stronger the self energy is, but the poorer the penetrating power is. Short wave UVC is completely absorbed by the ozone layer, the biological efficacy of long wave UVA is thousands times lower than that of medium wave UVB, and the aging capacity is far lower than that of UVB. It was found that most of the UVB is absorbed by the epidermis and is responsible for the decay of the epidermal cells, however, about 10-30% of the UVB penetrates the epidermis to reach the dermis and damage the fibroblast and cytoplasmic matrix.

Thus, skin photoaging can induce keratinization of the epidermis and aging of the dermis, and photoaging reduces synthesis of collagen and elastin of the skin, causing thinning of the dermis and deterioration of elasticity. In addition, ultraviolet light can also damage cellular DNA, lipids, and proteins by generating reactive oxygen species.

The mechanism of skin photoaging is still in the research demonstration stage. There are several well-established mechanisms of photoaging by current researchers: free radical injury theory, normal skin cell apoptosis, skin cell canceration, collagen degradation, collagen expression inhibition, etc. The currently widely accepted views are: ultraviolet irradiation causes free radical generation, which results in decreased cell viability and decreased cell synthesis and metabolic capacity. How to effectively resist skin damage caused by photo-aging has been a major problem in the industry.

Disclosure of Invention

The invention aims to provide a essence composition containing liposome capable of resisting photoaging injury, which can remove free radicals in skin and promote synthesis of collagen and elastin in skin, and is greatly favored by consumers.

It is another object of the present invention to provide a method for preparing a concentrate composition comprising liposomes that can combat photoaging damage.

In order to achieve the above object, the present invention adopts the following technical scheme:

the essence composition comprises liposome capable of resisting photoaging injury, and comprises the following components in parts by weight:

0.1-20 parts of liposome capable of resisting photoaging injury;

80-99.9 parts of cosmetic auxiliary material matrix.

Further, the essence composition comprises liposome capable of resisting photoaging injury, wherein the liposome capable of resisting photoaging injury comprises, by weight, 0.1-5 parts of a calli extract of alpine leontopodium, 0.1-10 parts of a lysate of a fermentation product of the schizosaccharomyces, 0.1-3 parts of an spartina extract and 0.01-1 part of carnosine.

The essence composition containing liposome capable of resisting photoaging injury comprises the following components in parts by weight:

0.1-7 parts of glycerin, 0.01-0.1 part of EDTA-2NA, 0.2-5 parts of 1, 3-propanediol, 0.1-3 parts of 1, 2-hexanediol, 0.2-4 parts of p-hydroxyacetophenone, 0.1-1 part of carbomer, 0.01-0.1 part of sodium hydroxide, 2-8 parts of isononyl isononanoate, 0.01-1 part of amino-terminal polydimethylsiloxane, 1-2 parts of squalane, 0.01-2 parts of vitamin E, 0.01-2 parts of sunflower seed oil and the balance of deionized water.

Preferably, the essence composition containing liposome capable of resisting photoaging injury comprises the following components in parts by weight:

2-18 parts of liposome capable of resisting photoaging injury, which mainly comprises the following components: 0.1-5 parts of a calli extract of alpine leontopodium, 0.2-10 parts of a lysate of a fermentation product of a two-split yeast, 0.1-2.5 parts of an spartina extract and 0.01-0.5 part of carnosine;

82-98 parts of cosmetic auxiliary material matrix, which mainly comprises the following components: 0.1-5 parts of glycerin, 0.02-0.08 part of EDTA-2NA, 1-3 parts of 1, 3-propanediol, 0.1-1 part of 1, 2-hexanediol, 0.2-1 part of p-hydroxyacetophenone, 0.1-0.8 part of carbomer, 0.01-0.08 part of sodium hydroxide, 4-7 parts of isononyl isononanoate, 0.02-0.09 part of ammonia-end polydimethylsiloxane, 1.2-2 parts of squalane, 0.01-0.1 part of vitamin E, 0.01-0.1 part of sunflower seed oil and the balance of deionized water.

Preferably, the liposome capable of resisting photoaging injury comprises the following main components in percentage by mass: lysates of fermentation products of Saccharomyces cerevisiae: sparassis crispa extract: carnosine=1: (1-3): (0.1-0.5): (0.01-0.05).

Preferably, the liposome capable of resisting photoaging injury is prepared by the following steps:

1) Weighing a certain amount of the calli extract of the alpine leontopodium herb, the lysate of the fermentation product of the two-split yeast, the spartina crispa extract and the carnosine in a phosphate buffer solution (pH=7.4), and performing ultrasonic dissolution;

2) The mass ratio is (2-4): 1, adding a proper amount of organic solvent into a beaker, stirring at the normal temperature at the rotating speed of 500-800 r/min for 0.1-0.5h until the organic solvent is completely dissolved, transferring the solution into a round bottom flask, and evaporating the organic solvent under reduced pressure to remove the organic solvent, wherein a layer of film appears on the inner wall of the flask;

3) Adding the phosphate buffer solution in the step 1) into the flask in the step 2) to fully hydrate the film, wherein the hydration temperature is 40-60 ℃, and placing the flask into an ultrasonic cleaner for ultrasonic treatment for 3-6 min after hydration;

4) And respectively passing the solution obtained after ultrasonic treatment through microporous filter membranes of 0.45m, centrifugally washing, and drying to obtain the liposome capable of resisting photoaging injury.

Preferably, the liposome capable of resisting photoaging injury is prepared from one or more of soybean phospholipid, lecithin and dipalmitoyl phosphatidylcholine. The organic solvent is one or more of ethanol, acetone and dichloromethane; the particle size of the liposome capable of resisting the photoaging damage is 80-200nm.

Preferably, the preparation method of the essence composition containing liposome capable of resisting photoaging injury comprises the following steps:

1) Dividing the various components forming the essence into four phases;

the phase A is water balance, 0.1-7 parts of glycerol, 0.1-0.1 part of EDTA-2NA0.01, 0.2-5 parts of 1, 3-propanediol, 0.1-3 parts of 1, 2-hexanediol, 0.2-4 parts of p-hydroxyacetophenone and 0.1-1 part of carbomer;

the phase B is 1 part of water and 0.01 to 0.1 part of sodium hydroxide;

the phase C is isononyl isononanoate 2-8 parts, ammonia-end polydimethylsiloxane 0.01-1 parts, squalane 1-2 parts, vitamin E0.01-2 parts and sunflower seed oil 0.01-2 parts;

phase D is 0.1-20 parts of liposome capable of resisting photoaging injury;

2) Heating the phase A to 80 ℃, and uniformly stirring (proper homogenization can be performed to help uniform dissolution of the carbomer);

3) Firstly, pre-mixing the phase B into transparent liquid, after the phase A is completely dissolved, starting to cool, cooling to 60 ℃, adding the phase B, and uniformly stirring;

4) Continuously and uniformly mixing the phase C, and slowly adding the mixture into a A, B phase;

5) After A, B, C phases are uniformly mixed, the temperature is reduced to 45 ℃ to add the phase D, and the mixture is uniformly stirred;

6) Discharging when the temperature reaches 40 ℃, and obtaining the essence composition containing liposome capable of resisting photo-aging injury.

In particular, the process for preparing a serum composition comprising liposomes that are resistant to photoaging damage, the serum composition prepared by the process having a pH in the range of 4-10, preferably a pH in the range of 5-8, and most preferably a pH in the range of 5-7.

Preferably, the essence composition containing liposome capable of resisting photoaging damage can be applied to skin care products, mainly used for essence milk, and also used for essence water, essence milk and essence oil products.

By means of the technical scheme, the invention has the following beneficial effects:

1) The novel liposome capable of resisting photoaging injury mainly comprises the following effective components: a alpine leontopodium callus extract, a two-part yeast fermentation product lysate, a spartina crispa extract and carnosine. The four functional components are compounded simultaneously, so that the skin can be effectively protected from being damaged by ultraviolet rays, degradation of hyaluronic acid, collagen and elastin in the dermis is avoided, skin microcirculation is promoted, cell activity is improved, and skin redness symptoms are reduced.

2) The liposome capable of resisting photoaging injury is innovated by the invention, and the active ingredients of the alpine leontopodium herb callus extract, the two-split yeast fermentation product lysate, the spartina crispa extract and the carnosine are used after being wrapped by the liposome, so that the transdermal effect of the active ingredients can be promoted more easily, the residence time of the active ingredients in the skin is prolonged, and the active ingredients can be slowly released at the active parts.

Drawings

FIG. 1 is a graph of ROS scavenging efficacy data for example 1;

FIG. 2 is a graph of fluorescence detection of ROS scavenging in example 1;

FIG. 3 is a graph showing the change rate of erythema index after using example 1 and comparative examples 1 to 15.

Detailed Description

The invention discloses a essence composition containing liposome capable of resisting photoaging injury. The main functional components of the essence composition comprise: the alpine leontopodium callus extract, the fermentation product lysate of the two-split yeast, the spartina spiralis extract and the carnosine have the capability of promoting the synthesis of skin collagen and removing skin free radicals. Through the preferred combination and efficacy test, it was found that the most preferred composition ratios achieved synergistic efficacy against photo-aging damage.

(1) Extract of calli of alpine leontopodium

The alpine leontopodium herb is also called as the edelweiss, is the mountain flower in Europe, has cold property, contains rich mineral substances, can clear heat and cool blood, and has the effects of soothing and calm, nourishing and protecting skin and resisting skin aging. In the case of UV resistance, it can inhibit the release of inflammatory factors, reducing DNA fragments broken by UVA and UVB irradiation; in addition, the composition can quickly resist the skin damaged by ultraviolet irradiation, promote the synthesis of the fiber of the dermis layer, inhibit free radicals and reduce the damage to the skin.

(2) Lysate of fermentation product of Saccharomyces cerevisiae

Consists of a bifidobacterium lysate obtained by biotechnology, comprising metabolites, cytoplasmic components, cell wall components and polysaccharide complexes, which are capable of promoting the UV irradiation of the natural DNA countermeasure mechanism, preventing double strand breaks, protecting the skin, keeping it viable, preventing UV-induced immunosuppression, and against UV-induced photoaging.

(3) Sparassis crispa extract

Sparassis crispa extract is derived from a broccoli mushroom. Such broccoli mushrooms are found in korea, japan, the united states and australia. Because of its ultra-high immunity-activating ability, it is called as "magic miracle mushroom" in japan. The common mushrooms grow on the shade, and the sparassis crispa needs more than 10 hours of irradiation every day, so that the sparassis crispa is the only 'sun mushrooms' in the world. Is extremely popular in Europe and America and Japan and has high price. Sparassis crispa also contains a large amount of beta glucan, antioxidant substances, vitamin C and vitamin E, and has good efficacy for removing skin problems such as melanin precipitation and the like as an effective component of a cosmetic product. Known as fantasy mushrooms, are used for the best food ingredients in france. Sparassis crispa is also used as a medicinal mushroom. Contains rich veratric acid. It can prevent and treat ultraviolet-induced DNA injury, support the antagonistic system of skin cells, reduce and prevent erythema caused by ultraviolet rays, and improve the local collagen level of skin.

(4) Carnosine (carnosine)

The skin naturally generates various peptide components, which are used for comprehensively resisting the damage of age, illumination, pressure and pollution to the skin, and the carnosine can well protect the skin from being damaged by solar radiation besides the traditional strong aging effect; in addition, it also protects and increases collagen production, and is a very potent quencher for uv-induced free radicals.

The raw material components used in the examples of the present invention are all commercially available products unless otherwise specified.

Examples 1 to 3

An essence composition containing liposome capable of resisting photoaging injury comprises the following raw materials in proportion as shown in table 1.

TABLE 1 raw material composition ratios (unit: parts by mass) of examples 1 to 3

The preparation process of the essence containing liposome capable of resisting photoaging injury in the embodiment 1 comprises the following steps:

1) Preparation of liposome a which can resist photoaging damage: weighing 1875mg of alpine leontopodium herb callus extract, 3750mg of a fermentation product lysate of the two-split yeast, 375mg of spartina crispa extract and 75mg of carnosine in a phosphate buffer solution (pH=7.4), performing ultrasonic dissolution, adding 200mg of phospholipid and 100mg of cholesterol into a beaker, adding a proper amount of ethanol into the beaker, stirring at a rotating speed of 600r/min for 0.2h to complete dissolution at normal temperature, transferring the solution into a round bottom flask, evaporating the organic solvent under reduced pressure, and forming a layer of film on the inner wall of the flask; adding phosphate buffer solution containing effective components into a flask with a film, fully hydrating the film at 50 ℃, and placing the flask into an ultrasonic cleaner for ultrasonic treatment for 3min after hydration; and respectively passing the solution obtained after ultrasonic treatment through microporous filter membranes with the diameter of 0.45 mu m, centrifugally washing, and drying for 12 hours to obtain the liposome capable of resisting photoaging damage, wherein the particle size of the liposome is 92.1+/-4.5 nm.

6) Discharging after the temperature reaches 40 ℃, and obtaining the essence composition containing liposome capable of resisting photo-aging damage, wherein the pH value of the essence composition is 5.8.

The preparation process of the essence containing the liposome capable of resisting the photoaging damage in the embodiment 2 comprises the following steps:

1) Preparation of liposome b which can resist photoaging damage: weighing 3850mg of alpine leontopodium herb callus extract, 7700mg of a fermentation product lysate of the two-split yeast, 770mg of spartina crispa extract and 154mg of carnosine in a phosphate buffer solution (pH=7.4), carrying out ultrasonic dissolution, taking 800mg of phospholipid and 200mg of cholesterol, adding a proper amount of acetone into a beaker, stirring at the normal temperature at the rotating speed of 700r/min for 0.4h to complete dissolution, transferring the solution into a round bottom flask, and evaporating the organic solvent under reduced pressure, wherein a layer of film appears on the inner wall of the flask; adding phosphate buffer solution containing effective components into a flask with a film, fully hydrating the film at 50 ℃, and placing the flask into an ultrasonic cleaner for ultrasonic treatment for 4min after hydration; and (3) respectively passing the solution obtained after ultrasonic treatment through microporous filter membranes with the diameter of 0.45 mu m, centrifugally washing, and drying for 18 hours to obtain the liposome capable of resisting photoaging damage, wherein the particle size of the liposome is 110.3+/-6.1 nm.

6) Discharging after the temperature reaches 40 ℃, and obtaining the essence composition containing liposome capable of resisting photo-aging damage, wherein the pH value of the essence composition is 5.7.

The preparation process of the essence containing the liposome capable of resisting the photoaging damage in the embodiment 3 comprises the following steps:

1) Preparation of liposome c which can resist photoaging damage: weighing 4175mg of alpine leontopodium herb callus extract, 8350mg of a two-split yeast fermentation product lysate, 835mg of spartina crispa extract and 167mg of carnosine in a phosphate buffer solution (pH=7.4), carrying out ultrasonic dissolution, adding a proper amount of dichloromethane into a beaker by taking 1000mg of phospholipid and 500mg of cholesterol, stirring at a normal temperature at a speed of 800r/min for 0.5h until the mixture is completely dissolved, transferring the solution into a round bottom flask, and evaporating the organic solvent under reduced pressure, wherein a layer of film appears on the inner wall of the flask; adding phosphate buffer solution containing effective components into a flask with a film, fully hydrating the film at 60 ℃, and placing the flask into an ultrasonic cleaner for ultrasonic treatment for 6min after hydration; and respectively passing the solution obtained after ultrasonic treatment through microporous filter membranes with the diameter of 0.45 mu m, centrifugally washing, and drying for 24 hours to obtain the liposome capable of resisting photoaging damage, wherein the particle size of the liposome is 122.4+/-5.9 nm.

6) Discharging after the temperature reaches 40 ℃, and obtaining the essence composition containing liposome capable of resisting photo-aging damage, wherein the pH value of the essence composition is 5.9.

Effect verification test example

1. Safety test

Test sample: example 1;

the testing method comprises the following steps: and testing the security by adopting a third party detection mechanism. The selected area is not more than 50mm ² A suitable patch device having a depth of about 1mm is provided by adding about 0.02 to about 0.025ml (g) of a subject to the patch device by a closed patch test method. The patch tester is attached to the forearm of a subject, the test object is removed after 24 hours, skin reactions are observed after 0.5 hour, 24 hours and 48 hours respectively, and the results are recorded according to the skin reaction grading standard in cosmetic safety technical regulations 2015.

TABLE 2 Patch test results

Remarks: skin reaction grading criterion

Level 0: a negative reaction;

stage 1: suspicious reactions; only weak erythema;

2 stages: weak positive response (erythema response); erythema, infiltration, edema, and possibly papules;

3 stages: strong positive response (herpes response); erythema, infiltration, edema, papules, herpes; the reaction may be beyond the test zone;

4 stages: extremely strong reactions (fusogenic herpetic reactions); obvious erythema, severe infiltration, edema, blepharospermia, and response beyond the test area.

As can be seen from table 2 above, the prepared essence showed no irritation and was safe.

2. Stability test

Test sample: example 1;

the testing method comprises the following steps: internal control standard, the appearance color, viscosity, pH for three months was observed.

TABLE 3 stability investigation results

Time

45℃

40℃

37℃

25℃

5℃

-15℃

CYCLE

Illumination of

1W

√

2W

√

3W

√

4W

√

2M

√

3M

√

From table 3 above, the prepared essence has no significant changes in color, viscosity and pH in three months under high and low temperature or light conditions, which indicates that our essence has good stability.

3. Evaluation of the anti-photoaging Effect of essence

Test sample: example 1 (experimental group) and blank carrier serum (control group: serum composition without liposome encapsulated efficacy ingredient, other ingredient content same as experimental group);

the testing method comprises the following steps: the culture was performed at 37℃for 18h after the use of the experimental group and the control group on the skin ex vivo. Then cultured at 37℃for 30min using an active oxygen assay kit, followed by 40j/cm using UVA ² The skin was irradiated for 2h30 min, and finally fluorescence was used to detect the active oxygen production of the dermal papilla layer, and the data were normalized according to the selected sites.

Test results:

as shown in fig. 1 and 2, the non-coated samples also have less active oxygen production, the active oxygen level of the non-coated samples is obviously improved after the skin is irradiated by ultraviolet rays, but after the non-coated samples are coated by an experimental group, the active oxygen level of the non-coated samples is obviously reduced by 36%, and the active oxygen level of the samples coated by a control group is not obviously changed, which can also be obtained from a fluorescence data chart, the active oxygen level of the non-coated samples is obviously improved after the non-coated samples are treated by ultraviolet rays, and the active oxygen level of the group coated with the samples of the experimental group is obviously reduced. The experimental group is illustrated to be a very potent ROS quencher.

4. Essence composition synergy experiment

A essence composition comprising liposome capable of resisting photoaging injury is prepared into essence, and the contents of the raw material components in comparative examples 1-15 are shown in the following tables 4-5.

Table 4 Table 1-7 shows the proportions of the components of the essences against photo-aging injuries (unit: parts by mass)

Table 5 Table of the proportions of the essential components (unit: parts by mass) of comparative examples 8 to 15 against photo aging damage

The preparation process of the essence for resisting photoaging damage comprises the following steps:

1) Heating the phase A to 80 ℃, and uniformly stirring (proper homogenization can be performed to help uniform dissolution of the carbomer);

2) Firstly, pre-mixing the phase B into transparent liquid, after the phase A is completely dissolved, starting to cool, cooling to 60 ℃, adding the phase B, and uniformly stirring;

3) Continuously and uniformly mixing the phase C, and slowly adding the mixture into a A, B phase;

4) After A, B, C phases are uniformly mixed, the temperature is reduced to 45 ℃ to add the phase D, and the mixture is uniformly stirred;

5) Discharging when the temperature reaches 40 ℃, and obtaining the essence composition capable of resisting photo-aging damage.

The effect of example 1 and comparative examples 1-15 of a synergistic experiment of the concentrate composition against photoaging damage was verified, the experimental procedure and results are as follows:

1. the tester: 48 female subjects, inclusion criteria: fitzpatrick skin type III volunteers.

2. The method comprises the following steps: samples were applied twice daily to the forearm, red spots were induced and recovered in the forearm (1.5 MED), once 20 minutes before uv irradiation, for six days, using a double blind test, and visually assessed.

3. Test results:

table 6 test set effect

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The ability to combat photo-aging damage is seen from the results in table 6: example 1> control 15> control 10-11> control 5-10> control 1-4. The essence composition containing the liposome capable of resisting photo-aging injury can effectively resist ultraviolet irradiation, can obviously reduce 29% of erythema when four main functional components are wrapped by the liposome and are used simultaneously, so that the functional components adopted by the essence composition can effectively protect UVB from the inside, can effectively protect and recover DNA from damage caused by ultraviolet rays, and are optimal components for resisting ultraviolet injury.

In addition, the four functional components are wrapped by liposome technology, so that the components can be efficiently delivered transdermally, and when the components are delivered to local skin, the components are not rapidly lost or degraded, but are retained in local skin for slow release, the utilization rate of the functional components is greatly improved, and meanwhile, the efficiency of the essence emulsion composition for resisting photoaging damage is also improved.

The present invention is not limited to the preferred embodiments, but can be modified, equivalent, and modified in any way without departing from the technical scope of the present invention.

Claims

1. A concentrate composition comprising liposomes that are resistant to photoaging damage, characterized in that: the essence composition comprises the following components in parts by weight:

0.1-20 parts of liposome capable of resisting photoaging injury; the main components of the liposome capable of resisting photoaging injury comprise 0.1-5 parts of alpine leontopodium herb callus extract, 0.1-10 parts of yeast fermentation product lysate, 0.1-3 parts of spartina crispa extract and 0.01-1 part of carnosine;

80-99.9 parts of cosmetic auxiliary material matrix;

the preparation method of the liposome capable of resisting the photoaging injury comprises the following steps:

1) Weighing a certain amount of the calli extract of the alpine leontopodium herb, the lysate of the fermentation product of the two-split yeast, the spartina crispa extract and the carnosine in a phosphate buffer solution with the pH value of 7.4, and carrying out ultrasonic dissolution;

2) The mass ratio is (2-4): 1, adding a proper amount of organic solvent into a beaker, stirring at the normal temperature at the rotating speed of 500-800 r/min for 0.1-0.5h until the organic solvent is completely dissolved, transferring the solution into a round bottom flask, and evaporating under reduced pressure to remove the organic solvent, wherein a layer of film appears on the inner wall of the flask;

4) And respectively passing the solution obtained after ultrasonic treatment through microporous filter membranes with the diameter of 0.45 mu m, centrifugally washing, and drying to obtain the liposome capable of resisting photoaging injury.

2. The concentrate composition of claim 1 comprising liposomes that are resistant to photoaging damage, wherein: the cosmetic auxiliary material matrix comprises the following components in parts by weight: 0.1-7 parts of glycerin, 0.01-0.1 part of EDTA-2NA, 0.2-5 parts of 1, 3-propanediol, 0.1-3 parts of 1, 2-hexanediol, 0.2-4 parts of p-hydroxyacetophenone, 0.1-1 part of carbomer, 0.01-0.1 part of sodium hydroxide, 2-8 parts of isononyl isononanoate, 0.01-1 part of amino-terminal polydimethylsiloxane, 1-2 parts of squalane, 0.01-2 parts of vitamin E, 0.01-2 parts of sunflower seed oil and the balance of deionized water.

3. The concentrate composition of claim 1 comprising liposomes that are resistant to photoaging damage, wherein: the essence composition comprises the following components in parts by weight:

4. A concentrate composition according to any one of claims 1 to 3, comprising liposomes that are resistant to photoaging damage, characterized in that: the liposome capable of resisting photoaging injury comprises the following main components in percentage by mass: lysates of fermentation products of Saccharomyces cerevisiae: sparassis crispa extract: carnosine=1: (1-3): (0.1-0.5): (0.01-0.05).

5. The concentrate composition of claim 1 comprising liposomes that are resistant to photoaging damage, wherein: the phospholipid is one or more of soybean phospholipid, lecithin and dipalmitoyl phosphatidylcholine; the organic solvent is one or more of ethanol, acetone and dichloromethane; the particle size of the liposome capable of resisting the photoaging damage is 80-200nm.

6. A process for the preparation of a concentrate composition comprising liposomes that can combat photoaging damage according to claim 1 or 5, characterized in that: the method comprises the following steps:

1) Dividing the various components forming the essence into four phases; the phase A is water balance, 0.1-7 parts of glycerol, 0.1-0.1 part of EDTA-2NA0.01, 0.2-5 parts of 1, 3-propanediol, 0.1-3 parts of 1, 2-hexanediol, 0.2-4 parts of p-hydroxyacetophenone and 0.1-1 part of carbomer; the phase B is 1 part of water and 0.01 to 0.1 part of sodium hydroxide; the phase C is isononyl isononanoate 2-8 parts, ammonia-end polydimethylsiloxane 0.01-1 parts, squalane 1-2 parts, vitamin E0.01-2 parts and sunflower seed oil 0.01-2 parts; phase D is 0.1-20 parts of liposome capable of resisting photoaging injury;

2) Heating the phase A to 80 ℃ and uniformly stirring;

7. The method of preparing a serum composition comprising liposomes that can combat photoaging damage according to claim 6, wherein: the resulting concentrate composition has a pH in the range of 4-10.

8. The method of preparing a concentrate composition comprising liposomes that are resistant to photoaging damage according to claim 7, wherein: the pH is in the range of 5-8.

9. The method of preparing a concentrate composition comprising liposomes that are resistant to photoaging damage according to claim 7, wherein: the pH is in the range of 5-7.

10. The method of preparing a concentrate composition comprising liposomes that are resistant to photoaging damage according to claim 7, wherein: the essence composition containing liposome capable of resisting photoaging injury can be applied to skin care products, and is mainly used for essence milk, essence water or essence oil products.