CN105613232A

CN105613232A - Blueberry tissue culture seedling transplantation substrate and preparation method thereof

Info

Publication number: CN105613232A
Application number: CN201510962644.3A
Authority: CN
Inventors: 李春雷; 刘明明; 刘海洋
Original assignee: QINGDAO WOLIN BLUEBERRY FRUIT INDUSTRY Co Ltd
Current assignee: QINGDAO WOLIN BLUEBERRY FRUIT INDUSTRY Co Ltd
Priority date: 2015-12-17
Filing date: 2015-12-17
Publication date: 2016-06-01
Anticipated expiration: 2035-12-17
Also published as: CN105613232B

Abstract

The invention discloses a blueberry tissue culture seedling transplantation substrate. The substrate comprises components in parts by volume as follows: 15-20 parts of moss, 1-5 parts of turf, 15-20 parts of vermiculite, 1-5 parts of fern rhizomes, 10-15 parts of wood shavings and 5-10 parts of seaweed fertilizer. The invention further discloses a preparation method of the blueberry tissue culture seedling transplantation substrate. The method comprises steps as follows: the moss is soaked with a bactericide for disinfection; the fern rhizomes are cut into sections, the moss, the turf, the vermiculite, the wood shavings and the seaweed fertilizer are added in proportion, and the components are mixed. The substrate has simple components, is reasonable in matching, environment-friendly, low in cost, economical, practical and simple to prepare, cannot scatter easily and has good economic benefit, the transplantation survival rate of tissue culture seedlings is greatly increased, and the rooting survival rate is 90% or above.

Description

A kind of blueberry tissue culture transplantation of seedlings substrate and preparation method thereof

Technical field

The present invention relates to blue berry seedling planting technology field, be specifically related to a kind of blueberry tissue culture transplantation of seedlings substrate and preparation method thereof.

Background technology

Blue berry belongs to Ericaceae genus vaccinium, and cultivation starts from the U.S., and the work of introducing a fine variety of China's blue berry starts from the middle and late stage eighties in 20th century. With tradition cuttage breeding method the difference is that, blueberry tissue cultivates breeding, it is possible to breed out a large amount of nursery stock at short notice, so receiving much concern. Current blueberry tissue cultivates one of fast breeding Main Means having become as blue berry seedling-wood breeding. But in blueberry tissue culture transplantation of seedlings to hole tray process, being generally adopted traditional host material, proportioning process is loaded down with trivial details, the harm that the existence of environment is certain, and plant percent is not high yet.

Summary of the invention

For the deficiency that above-mentioned prior art exists, the present invention provides a kind of blueberry tissue culture transplantation of seedlings substrate and preparation method thereof.

For achieving the above object, the present invention adopts the following technical scheme that a kind of blueberry tissue culture transplantation of seedlings substrate, is made up of the component of volumes below number: lichen 15-20 part, peat composed of rotten mosses 1-5 part, Vermiculitum 15-20 part, Rhizoma pteridii latiusculi 1-5 part, wood shavings 10-15 part, alga fertilizer 5-10 part.

Preferably, described blueberry tissue culture transplantation of seedlings substrate, it is made up of the component of volumes below number: 17 parts of lichen, the peat composed of rotten mosses 3 parts, Vermiculitum 17 parts, Rhizoma pteridii latiusculi 3 parts, wood shavings 13 parts, alga fertilizer 7 parts.

The preparation method that invention additionally discloses a kind of blueberry tissue culture transplantation of seedlings substrate, uses antibacterial soaking disinfection by lichen; By the Rhizoma pteridii latiusculi section of chopping, add lichen, the peat composed of rotten mosses, Vermiculitum, wood shavings and alga fertilizer according to aforementioned proportion, mix.

Preferably, described lichen soaks 5-8 hour with the chlorothalonil of 1000 times.

Preferably, described Rhizoma pteridii latiusculi is cut into broken section of 2-10cm length.

Under certain environment and nutritional condition, the physicochemical property of the transplanting medium of tissue cultured seedling determines the content of moisture, Nutrient absorption performance and air, and blueberry tissue culture Seedling is brought out Xin Gen and surviving and plays a key effect by the breathability of its mesostroma, moisture retention, acid-base value and foster grading factors.

The good water-retaining property of lichen, but humidity is too big, and the root being easily caused Seedling occurs to rot, and peat composed of rotten mosses good water-retaining property is more containing organic nutritional labeling, but viscosity is too big, and aeration is relatively poor, and Vermiculitum water conservation, breathability are better, but lacks nutrient. Rhizoma pteridii latiusculi quality is loosened, and water-retaining property is poor, but good permeability, resistance to rotten, containing certain nutrient. Wood shavings, draining well, not easily mouldy, without pathogenic bacteria, without insect hide, quality is light.

Blue berry belongs to shallow root system plant, and the conditions of existence such as soil, moisture is very harsh. Blueberry tissue culture Seedling is transplanting early stage, and the ventilative of substrate and moisture retention are required higher by tissue cultured seedling, and after restoration ecosystem, matrix nutrition composition are had higher requirements. Therefore, when selecting collocation substrate, select lichen and Vermiculitum collocation, by the permeation functions of Vermiculitum, enable the high humility of lichen to be spread, by suitable proportioning, both can meet the water suction requirement of substrate, and tissue cultured seedling butt rot will not have been caused again because humidity is excessive and dead, make tissue cultured seedling root be breathed freely fully, promote blueberry tissue culture Seedling to take root at wound healing position, be conducive to improving cuttage survival rate. Select the peat composed of rotten mosses and Rhizoma pteridii latiusculi collocation simultaneously, owing to nutrient is sufficient, for supplying nutrient after blueberry tissue culture Seedling restoration ecosystem, be conducive to the growth of anaphase blade, promote that photosynthesis produces. Owing to Rhizoma pteridii latiusculi is not perishable, and the peat composed of rotten mosses can be reduced with the distributions of broken section and harden and the risk that makes breathability reduce, be conducive to the elongation of later stage root system, meet the nutritional requirement of later stage growth of seedling. Wood shavings not only improve the porosity of substrate, substrate is promoted to breathe freely water conservation, substrate pH value can also be regulated, the pH value of substrate is required significantly high by blue berry growth, the pH value to substrate that adds of wood shavings plays cushioning effect, be conducive to the growth of blueberry tissue culture shoot root system, improve survival rate and stable growth state, contribute to obtaining the blueberry tissue culture Seedling of well developed root system. By the collocation of alga fertilizer, lichen and Rhizoma pteridii latiusculi, the nutrient that blue berry is taken root can not only be provided, moreover it is possible to the hole providing blue berry to take root, promote the rooting rate of blueberry tissue culture Seedling and survival rate to be greatly improved.

The invention has the beneficial effects as follows: the matrix components of the present invention is simple, collocation is reasonable, and not easily fragmentary, environmentally friendly, with low cost, economic and practical, survival rate that tissue cultured seedling transplant is greatly improved, root survival is up to more than 90%, and preparation process is simple, simplifies the preparation steps of substrate, improve production efficiency, greatly reduce the raw material harm to environment, there is good economic benefit.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further described.

Embodiment 1

A kind of blueberry tissue culture transplantation of seedlings substrate, is made up of the component of volumes below number: 15 parts of lichen, the peat composed of rotten mosses 1 part, Vermiculitum 15 parts, Rhizoma pteridii latiusculi 1 part, wood shavings 10 parts, alga fertilizer 5 parts.

The preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate, soaks 5 hours in order to sterilize by lichen with the chlorothalonil of 1000 times; Rhizoma pteridii latiusculi is cut into broken section of 2-10cm length, adds lichen, the peat composed of rotten mosses, Vermiculitum, wood shavings and alga fertilizer according to aforementioned proportion, mix.

The substrate pH of leaching solution of the present embodiment is 4.7; The individual plant cost of substrate is 0.05 yuan/strain. Using Lai Gexi (legacy) as experiment kind, in cuttage on April 15, start to take root after 15 days, rooting rate reaches 93%, and after 70 days, root length reaches 4.6cm.

Embodiment 2

A kind of blueberry tissue culture transplantation of seedlings substrate, is made up of the component of volumes below number: 20 parts of lichen, the peat composed of rotten mosses 5 parts, Vermiculitum 20 parts, Rhizoma pteridii latiusculi 5 parts, wood shavings 15 parts, alga fertilizer 10 parts.

The preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate, soaks 8 hours in order to sterilize by lichen with the chlorothalonil of 1000 times; Rhizoma pteridii latiusculi is cut into broken section of 2-10cm length, adds lichen, the peat composed of rotten mosses, Vermiculitum, wood shavings and alga fertilizer according to aforementioned proportion, mix.

The substrate pH of leaching solution of the present embodiment is 4.9; The individual plant cost of substrate is 0.04 yuan/strain. Using Lai Gexi (legacy) as experiment kind, in cuttage on April 15, start to take root after 15 days, rooting rate reaches 94%, and after 70 days, root length reaches 4.8cm.

Embodiment 3

A kind of blueberry tissue culture transplantation of seedlings substrate, is made up of the component of volumes below number: 17 parts of lichen, the peat composed of rotten mosses 3 parts, Vermiculitum 17 parts, Rhizoma pteridii latiusculi 3 parts, wood shavings 13 parts, alga fertilizer 7 parts.

The preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate, soaks 7 hours in order to sterilize by lichen with the chlorothalonil of 1000 times; Rhizoma pteridii latiusculi is cut into broken section of 2-10cm length, adds lichen, the peat composed of rotten mosses, Vermiculitum, wood shavings and alga fertilizer according to aforementioned proportion, mixed.

The substrate pH of leaching solution of the present embodiment is 4.5; The individual plant cost of substrate is 0.04 yuan/strain. Using Lai Gexi (legacy) as experiment kind, in cuttage on April 15, start to take root after 15 days, rooting rate reaches 95%, and after 70 days, root length reaches 5cm.

Finally should be noted that; above example is only in order to illustrate technical scheme; but not limiting the scope of the invention; although having made to explain to the present invention with reference to preferred embodiment; it will be understood by those within the art that; technical scheme can be modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention.

Claims

1. a blueberry tissue culture transplantation of seedlings substrate, is characterized in that, is made up of the component of volumes below number: lichen 15-20 part, peat composed of rotten mosses 1-5 part, Vermiculitum 15-20 part, Rhizoma pteridii latiusculi 1-5 part, wood shavings 10-15 part, alga fertilizer 5-10 part.

2. a kind of blueberry tissue culture transplantation of seedlings substrate as claimed in claim 1, is characterized in that, be made up of the component of volumes below number: 17 parts of lichen, the peat composed of rotten mosses 3 parts, Vermiculitum 17 parts, Rhizoma pteridii latiusculi 3 parts, wood shavings 13 parts, alga fertilizer 7 parts.

3. the preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate as claimed in claim 1 or 2, is characterized in that, lichen is used antibacterial soaking disinfection; By the Rhizoma pteridii latiusculi section of chopping, described in claim 1 or 2, ratio adds lichen, the peat composed of rotten mosses, Vermiculitum, wood shavings and alga fertilizer, mixes.

4. the preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate as claimed in claim 3, is characterized in that, described lichen soaks 5-8 hour with the chlorothalonil of 1000 times.

5. the preparation method of a kind of blueberry tissue culture transplantation of seedlings substrate as claimed in claim 3, is characterized in that, described Rhizoma pteridii latiusculi is cut into broken section of 2-10cm length.